LOCUS       p60512-IMP             24306 bp    DNA     circular   BCT 16-FEB-2018
DEFINITION  Pseudomonas aeruginosa strain 60512 plasmid, complete sequence.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 24306)
  AUTHORS   Zhan,Z., Hu,L., Jiang,X., Zeng,L., Feng,J., Wu,W., Chen,W.,
            Yang,H., Yang,W., Gao,B., Yin,Z. and Zhou,D.
  TITLE     Plasmid and chromosomal integration of four novel blaIMP-carrying
            transposons from Pseudomonas aeruginosa, Klebsiella pneumoniae and
            Enterobacter spp
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24306)
  AUTHORS   Zhan,Z., Hu,L., Jiang,X., Zeng,L., Feng,J., Wu,W., Chen,W.,
            Yang,H., Yang,W., Gao,B., Yin,Z. and Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2018) Anhui Medical University, No.81,Meishan
            Road,Shushan District, Hefei, Anhui 230032, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 3.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24306
                     /organism="Pseudomonas aeruginosa"
                     /plasmid=""
                     /mol_type="genomic DNA"
                     /strain="60512"
                     /country="China"
     CDS             1..1119
                     /gene="repA"
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation protein RepA"
                     /translation="MNNERGKGPQQIGEALGDLDKSLARLKAAHDAQRQKRIEAAARP
                     GESWEQAAARLREEEARAERERSSGSVKSPPPASSIRKPPRGDEQPDFFVPTLYDVGT
                     KDSRSIMDVAVFRLSKKDRRAGEVINYTLPDGHVTVSAGAAGMASVWDYDLVLMAVSH
                     LTESMNRYREGKGAKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAVQ
                     RTRKAKNGRTVTVSEGEALISRYKVIKSDSGKPEYIEIELADWMYREITEGKNPDVLT
                     VHPDYFLIDPGIGRFVYRLARRAAGKSAAKWAFQTIYDRSGSAGKFKEFCRILRNIIQ
                     ANDLPEYELREEAGQGGPLLVMTHRDHADAELPPPAGV"
     repeat_region   1205..1222
                     /gene="iterons"
                     /product="RepA-binding sites; regulation of replication"
     repeat_region   1226..1243
                     /gene="iterons"
                     /product="RepA-binding sites; regulation of replication"
     repeat_region   1247..1264
                     /gene="iterons"
                     /product="RepA-binding sites; regulation of replication"
     repeat_region   1268..1285
                     /gene="iterons"
                     /product="RepA-binding sites; regulation of replication"
     misc_feature    complement(1568..2029)
                     /gene="Δorf498"
                     /product="Hypothetical protein"
     CDS             2045..2953
                     /gene="kfrA"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein KfrA"
                     /translation="MAVTKEQIFSAADELAAGGQKPTLEAIRQITGGSYTTISPALNE
                     WKARQVTAAAPLREPAPQAVADRLAELGSDLWTIALDLANARLAVEREGLEKARAELE
                     ADRAEATELADRLAAQVDDLQSRLASIEAAEAAARGEADELRGQLATAQEQAHTAEAR
                     AQEIERRAGELRTELDRAHQDADQARGALAEQQKASQAAAAQLDQVRGELAKVQAKAQ
                     ADQEAQQEQRKQAAAEAHRMAERLTAAQAERDQATKAAAQAREDAARLAGQLDTLKEQ
                     SAALLARITPPAAEVKPTRKKPGSGE"
     CDS             complement(3125..3517)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MAGIIAAVLGVIALAWFLRRLFPALFAAVFVLAAPAWLVGGLLL
                     DLLRRPLAFLRPLGLGLARLVGFVLVLPLLPFIFAWSFFAELWRQFRPKASPAPAGVK
                     PPTPMQAAARRTADVIDLAAFRQRHKKE"
     CDS             complement(3528..6050)
                     /gene="cpl"
                     /codon_start=1
                     /transl_table=11
                     /product="Coupling protein Cpl"
                     /translation="MDVREFLARLWAAVVAVFFGAFVLSLANTAHARVFPECNPAAEA
                     AKFYGAEDADAWVKRVCDAQEAAFQKWENSLQALDVGQQDLAMATNAGNWQAYRDKWA
                     ELLPVLKELEAAALANRNAPGAANILSLYRNDLGLFLQNAGLSSAGSLDDFSDRILAG
                     LDGERPAAAATAGVNVVQQSVTRGVEFGKGIGTAQAETILAEYRAQVQQRGAERMEQL
                     GGSTVSGYFGGFGERIKAAFGGWLFYLVTVCFVGAWLAHKRRQNPVTVGTAVGLAFLV
                     PSVGMLLLFVFAPFIPGWFILAATVAGTVATYLNAGRVFGWLAGLVGAESPTGKRLRI
                     LGGTIENLRGQVQPSGPVTAAGAAVAAVDNSSTHPLGSHGSARWGTVDEIRQGGHLVA
                     PGKPGGFALGRVSDAPAGLDQRFRFTGHVVTVAPTGSGKGIGAVIPNLLDYPGSALVL
                     DVKGENAAVTARARRALGQAVYSVDPFAVNGDGGAAFNVLDRLDVWNPDCVSESAILA
                     DALVIAESKGDAVHFDESAKNFLQGLMLHVAGLDDTERRNLGELRRLLTAGEAEFFDV
                     LGAMAADDTAAFGIPARAANTLMGMADKERGSVLSTARRNTAFLDDPRVSAALSRSDF
                     DLSEIKATAMTVYLVMPANRIGPNARFLRLFISSVIAAITSSNVQPAHRVAFLLDEFG
                     QLGYMKQIEDAVSLLRGYGLAFWVFIQDLSQLKGTYPKWQTFLANSAKSFFGTDDYDT
                     AKYISDSLGKATIEFETENTGKNSGSGLSGGGGSMNRGKSTGTSQQFTGRELLTPDEV
                     MRLGPEHPIVLVRGERPYLLDRLNYLADAEYAGRFDANPYHS"
     CDS             complement(6085..6306)
                     /gene="traD"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type type IV secretion protein TraD"
                     /translation="MNARREDARRKIQLGGLVIKSGMAGYPVSVILGALTLAAGALNG
                     PNADATKARFEAAGDEAFSDDGEDGGNRS"
     CDS             complement(6329..6586)
                     /gene="traC"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type type IV secretion protein TraC"
                     /translation="MTMARKTLSERKADKLAELNRIKAELAELESKAAERIGKIALSA
                     GLGDLDIEDAELRKEFEAIASKFRKSEGKQAAPMAATPSAD"
     CDS             6772..10119
                     /gene="traA"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type type IV secretion protein TraA"
                     /translation="MAIYHLNTHTIGRAAGHSAVAAAAYRSASSLVDERTGEVFDFTR
                     KGGVLSSEIVTPAGVPVPERAALWNAAEAAEKRKDARVAREWRAALPHELNEADRKEL
                     ATQMGQAIADRYGVAVDVYIHAPDKEGDDRNFHVHMLATTRTIGADGTLGAKAVIELA
                     NKDRQKAGIPGTSQGDIIEIRQQWAELTNEALERAGISARVDHRSYADQGVELTPTKH
                     IGSDAVAMDRRGLEADRIDIHNADRQEQARQIVERPEIILDKLTATQAVFTRRDIAAE
                     LNRYIDDADQFQGLLARLEQSPLLVELEPASGRDPAKFSTREMIDTERGMVDSAERLA
                     RTGRHGVSSPITNAAIDGAGTLSAEQQNAVRHVLKPGSLAVVIGDAGTGKSFSMKVAR
                     EAWQAQGFNVRGAALAGKAADELQAGSGIDSRTLASLEFAWKNGKDKLTSRDVLVIDE
                     AGMIGSRQLGRVLKAAEQAGAKVVLLGDDKQLAAIEAGAAFRGVVQHVGAAEITEVRR
                     QKEAWARAAGQELARGSVADGLAAYAERGYVKIHDSRDAARDSLAAAYVGDQGKGSQI
                     ILAHSNKDVQALNEAVRQARKERGELRGTARFMTERGGREFAPGDRIVFLKNDRDLGV
                     KNGTLGTVEHSEDGSLAVRLDSGEARRFQAAQYAAVDHGYAVTIHKAQGVTVDRAYLL
                     ATPGMDRSLAYVGMTRHREAATLFAGADDFTDRRVGRLVDHGAAPYENKPENRASYFA
                     TLENDKGERHTIWGVDLERAIAASGAKRGDRIGLEHGGSETVRLPDGTTAERNTWHVR
                     GAAELAAGKLAQVLGRERPKESTLDFADRRGFDGESVMRRWLERGRAKVAELAGKMRD
                     GLRRNLERHGRPDLMPATDIAGTPTVQRPQQIERTPEDEKKRRAAEIAAKFRQQVSEE
                     RGEQVAPQQRPQAERPAPDPLAGFRAGVERAEAAGGGLALDVAKAQLAVAQEFHAAGK
                     DPRHHSAAIMQEGQQRAFAGLAQPSQAKAEPERPAAVQVSEQDRKRAEAHAKMEVSQF
                     RALAVKRQAGFAGYSDRNPSAWRELPDELRGRIERFNAMPKERQAVELDKMQRELADR
                     YARNPQEITRSRQRQREQERGRDGFSR"
     CDS             10204..10566
                     /gene="hsdR"
                     /codon_start=1
                     /transl_table=11
                     /product="Type I restriction enzyme HsdR"
                     /translation="MSSTEPTNEELAKGYAKQIEQAQERREAKDLERLERHGRAAAQQ
                     SQAIEAEQSGQGVELDASQKVELNEINGQQAKALAKELNRDNENGDQVKQARAREIAE
                     RFKEKAAKDREQSRSFSR"
     CDS             10639..10860
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MATCNVTGGTRRDLTKYRITFAVSLAVLIGTGGMFWHAYRTHAG
                     WLNSGDVAVRDAIMDGTIQPPADGKGGAR"
     CDS             10857..11762
                     /gene="degP"
                     /codon_start=1
                     /transl_table=11
                     /product="Trypsin-like serine proteases DegP"
                     /translation="MKAQTWRRTALAAYILAGASLTVAGYTVATTEANANALYRFAEH
                     DNDAYSARSRSIAEGITAAALRGQGDGVPDAADWPRIIPQVIPSAVNVAALPFVTRPD
                     DESRVWLVPGVQSNPATAALGAVRFWLKSRDQEPRHWQTIGSGAFIADGRYVLTAAHV
                     IDGKSGYRVQTAAGRWLDAELIGLDLRRDVGVLRVSEPGQPIKTGAMPIQGQPVLTIG
                     APGGRGFSVSVGIVSRYAESARFSADEALQIDAPVTGGNSGGLVVSARGEAVGVVSHS
                     DAAFTQAVPIGTALAVATELIERSR"
     CDS             11858..12196
                     /gene="orf339"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MKLRNSFPLAAVAALLMASTAHAGQDADASRFSIMAPVQAAYDA
                     YQVTASRAQNTMDSIEAELREPGLSAERRELLAVSLATLRAREAAALERLHAESALAQ
                     LKMADWNASR"
     CDS             complement(12259..12465)
                     /gene="chpS"
                     /codon_start=1
                     /transl_table=11
                     /product="Antitoxin protein ChpS"
                     /translation="MSRPRYTLDELLAESDYSQPLPPEEREWVDAPAVGREWPACETL
                     EGEGLANIASPDDDGRDGGSHRQK"
     CDS             complement(12462..12638)
                     /gene="relB"
                     /codon_start=1
                     /transl_table=11
                     /product="Antitoxin protein RelB"
                     /translation="MTNKAATISAAVPANVKAEAAAVAVAHGMSLAALVRELVARVAA
                     HDAETLAWLDEARR"
     CDS             complement(12635..13564)
                     /gene="orf930"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MTDLVTALRDFNKDRKFNRKGPLCVALVVTQHARKGLPLNPDEL
                     LTEAGGQVLGLGRGAVQAVLNRHDITRVLAAEGGRTSRGSISNMREYVAFLNGLAAGG
                     TVDLDAVEAFWIERVHEFFSAKPFKIRLDASRSLRTLVRDMIAQAEERQRNTPGMQYA
                     GAVLQHLVGAKLDCALGAGNFDHNSFSTSDAQTGRNGDFFIGDVAIHVTTAPGEAVIG
                     RCRDNIDDGHRPIIVTTGRGLTVAEGLAENAGLGERIDVFEVEQFIALNLYELGKFAA
                     EGRRVAVGDVVTRYNEIVEEVETDPSLKIEFRQ"
     CDS             complement(13564..14712)
                     /gene="dcm"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methylase Dcm"
                     /translation="MANFYEFFAGGGMARAGLGPDWQCLLANDFDPKKAASYAANWGA
                     DHLRVGDVAALTTADLPAGADLAWASFPCQDLSLAGAGAGLKGDRSGTFWPFWQLIKA
                     LGDEDRAPRLVVLENVCGALSSHDGKDFAAISSALSNNGYRFGAVVINAVHFLPQSRP
                     RLFIIGVRKSSPIPRALVASGPEGEWHPSALVEAYGKLSKRAQSAWEWWRLPAPPART
                     SIFADLVEDKPHGVTWHTAAETKKLLGMMNPLNLAKVEAAKKAGGRQVGTIYKRTRAD
                     GPNGEKVQRAEIRFDDVAGCLRTPTGGSSRQTIMVIEGKRVRSRLLSPREAARLMGLP
                     DTYALPKNYNDAYHLAGDGVAVPVVRFLAAHILEPLLAAALAVEKKAA"
     CDS             complement(14705..14926)
                     /gene="hicB"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain protein HicB"
                     /translation="MAPMKADRKDSRSSALERINLRLTPEVLKAIDAECSRRAGSVSR
                     NTWITEAVTERLGRLASSSHPQGAGGANG"
     repeat_region   15002..15006
                     /gene="DR_Tn6394"
                     /product="Tn6394 direct repeat; target site duplication"
     mobile_element  15007..19988
                     /gene="In992"
                     /product="Insertion sequence: In992"
     repeat_region   15007..15031
                     /gene="IRt_In992"
                     /product="In992 inverted repeat at the tni end"
     misc_feature    15148..15579
                     /gene="ΔtniA"
                     /product="Truncated DD(35)E transposase TniA"
     misc_recomb     15549..15675
                     /gene="attC_IMP-1"
                     /product="attC site for IMP-1"
     CDS             complement(15678..16418)
                     /gene="blaIMP-1"
                     /codon_start=1
                     /transl_table=11
                     /product="Metallo-beta-lactamase IMP-1"
                     /translation="MSKLSVFFIFLFCSIATAAESLPDLKIEKLDEGVYVHTSFEEVN
                     GWGVVPKHGLVVLVNAEAYLIDTPFTAKDTEKLVTWFVERGYKIKGSISSHFHSDSTG
                     GIEWLNSRSIPTYASELTNELLKKDGKVQATNSFSGVNYWLVKNKIEVFYPGPGHTPD
                     NVVVWLPERKILFGGCFIKPYGLGNLGDANIEAWPKSAKLLKSKYGKAKLVVPSHSEV
                     GDASLLKLTLEQAVKGLNESKKPSKPSN"
     misc_recomb     16429..16540
                     /gene="attC_aacA7"
                     /product="attC site for aacA7"
     CDS             complement(16557..17015)
                     /gene="aacA7"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 6'-N-acetyltransferase AacA7"
                     /translation="MDSSPLVRPVETTDSASWLSMRCELWPDGTCQEHQSEIAEFLSG
                     KVARPAAVLIAVAPDGEALGFAELSIRPYAEECYSGNVAFLEGWYVVPSARRQGVGVA
                     LVKAAEHWARGRGCTEFASDTQLTNSASTSAHLAAGFTEVAQVRCFRKPL"
     misc_recomb     17019..17081
                     /gene="attI1"
                     /product="attI1 site"
     CDS             17162..18175
                     /gene="intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="IntI1 integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     regulatory      17249..17277
                     /gene="PcW"
                     /product="Promoter PcW"
     regulatory      17249..17254
                     /gene="-10_PcW"
                     /product="-10 region of PcW"
     regulatory      17272..17277
                     /gene="-35_PcW"
                     /product="-35 region of PcW"
     mobile_element  18277..19893
                     /gene="ISCfr1"
                     /product="Insertion sequence: ISCfr1"
     repeat_region   18277..18301
                     /gene="IRR_ISCfr1"
                     /product="ISCfr1 inverted repeat right"
     CDS             complement(18442..19812)
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCfr1 transposase"
                     /translation="MMGQLPGGQQRLFYSFNLEDHVPAQHLLRSIDQCLDLSDLRAYL
                     ADFYSPIGRPSIDPELMVRMLVVGYCYGIRSERRLCEEVHLNLAYRWFCRLGLEDEVP
                     NHSTFSKNRHGRFRDSDLFRWLFNEVLRRCMAAGLVKGEGFAVDASIIKADASRQRGV
                     AGDEVDWNDPKLSSRAVREYLEALDEEALAEALPKKISLTDPQSRWTAAPGGPAFFAY
                     STNYLIDTEHGVIMDVEATPAHRTAEVDSTRTMVERVKAQFDLTPERLIGDTAYGTAP
                     MLAWMVEEKDIEPHVPVWDKTERKDDSLSSNDFHWNQEANEYRCPAGKPLRSEWRAFT
                     QKRSRVTKAHTIIYRSSQTDCTTCPLKAKCCPNTPNRKIVRSIHEAARDVARRIAKTP
                     EYLVSRCERKKVEMLFAHLKRIMKLDRLRLRGLTGATDEFTLAAAVQNLRRMAKLLPQ
                     GPPLTG"
     repeat_region   19869..19893
                     /gene="IRL_ISCfr1"
                     /product="ISCfr1 inverted repeat left"
     repeat_region   19964..19988
                     /gene="IRi_In992"
                     /product="In992 inverted repeat at the integrase end"
     mobile_element  20102..22246
                     /gene="ISPa17"
                     /product="Insertion sequence: ISPa17"
     repeat_region   20102..20126
                     /gene="IRL_ISPa17"
                     /product="ISPa17 inverted repeat left"
     CDS             complement(20133..20816)
                     /codon_start=1
                     /transl_table=11
                     /product="Putative recombinase"
                     /translation="MSVKRSLVGHAGHCPAKLLHNGLSDIKIGGRNDGQERRQMALIG
                     YARVSTAEQDTALQTDALRKAGCERVFEDTASGAKADRPGLADALAYLRDGDVLAVWR
                     LDRLGRSMPHLIETIGALEARGVGFRSLTEAIDTTTPGGRLIFHVFGALGQFERDLIR
                     ERTKAGLTAAAARGRKGGRKPVVTADKLQRAREHIANGLNVREAATRLKVSKTALYTA
                     LQSTSAADS"
     CDS             20881..21132
                     /gene="mazE"
                     /codon_start=1
                     /transl_table=11
                     /product="Toxin/antitoxin system antitoxin protein MazE"
                     /translation="MTTLTVTARGQVTFRKDVLQHLGIRPGDKIELDLLPDGRGVLKA
                     ARPAGTIASFVGLLAGKTQKVATIEEINEAAAQGWAGKQ"
     CDS             21129..21524
                     /codon_start=1
                     /transl_table=11
                     /product="Toxin/antitoxin system toxin component, PIN
                     family"
                     /translation="MKVAVDTNVLVRAVVRDDPAQADVAAAVLTDAELIAVALPCLCE
                     FVWVLLRVYGFQQADAASAIRALLAAANVEVNRPAVEAGLLVLDAGGDFADGVIAYEG
                     NWLGGETFVSFDKKAVALLTAQGQSTRLL"
     CDS             21530..22072
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISPa17 transposase"
                     /translation="MSMNEFRRLAAKIDQHMQQLAAQGVSEAHAIINRMMGYGPDLHR
                     IWVGTSDQQLMALSREFPGFYRYARIMEEASEAERRKASRPYDGMAEFSEQHKQMGAQ
                     LLTTAATLERGYQAFRASGSLQDFRPQLDELGRLHRQWLSDLEAFKDSLRTQGAEPKV
                     LEYVNEAFGRLAERIKQLAG"
     repeat_region   22222..22246
                     /gene="IRR_ISPa17"
                     /product="ISPa17 inverted repeat right"
     repeat_region   22247..22251
                     /gene="DR_Tn6394"
                     /product="Tn6394 direct repeat; target site duplication"
     CDS             22339..23004
                     /gene="pinE"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA specific recombinase PinE"
                     /translation="MKDKPMIGYARVSTDDQDLTNQRAELHAAGCTRIFAEKITGAHA
                     KRPELGRMLDHLRAGDVVTVTRLDRLARSTRDLLDIAEQLQAKGAGLRSLAEPWADTT
                     TPAGRMVLTVFAGIAEFERSLIIDRTRNGREAAKARGVQFGRRPTLTPGQVEHARELL
                     GAGRTAAETAELLGVHRATLYRALRGQETSRAEARRRGAFVEDALTEADALAAADQEG
                     MKA"
     CDS             23001..23294
                     /gene="orf294"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MRNGHYPLPPLRPELQAAHDEMDAKGDYFAYAVCRTHDGQAWEV
                     TTRQGGMYGALDGRYLDHDEAAAAGVAWLLEQLDREPTADEATYRALWESMGK"
     CDS             23376..24008
                     /gene="parA"
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid partitioning protein ParA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLDGADVLLVDSDPQGSA
                     RDWAAVREDQPLTVVGIDRPTIDRDVKNVARKVDFVVIDGAPQAADLAVSAIKAADFV
                     LIPVQPSPYDIWATADLVELVKQRIEVTDGKLQAAFVVSRAIKGTRIGGEVADALAGY
                     ALPILDARITQRVSYPGTAAAGTTVLETEPDGDAAAEVRALAAEIKQKLN"
     CDS             24029..24253
                     /gene="parB"
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid partitioning protein ParB"
                     /translation="MSKPTTTLTAGRPSARTDKARAATLASLADDAGGMKRVNFELPA
                     EQHAKLKIYATKQGKSIKDVLSEYVASLPD"
BASE COUNT     4828 a   7649 c   7584 g   4245 t
ORIGIN
        1 atgaacaacg agcgcgggaa ggggccgcag cagatcggcg aggccctggg cgacctggac
       61 aaatcacttg cgaggttgaa ggccgcgcac gacgcgcagc gccagaagcg catcgaggcg
      121 gccgccaggc cgggcgaatc ctgggagcag gccgccgcca gactgcgcga ggaagaagct
      181 agggccgagc gtgagcgcag ttccggctcc gttaaatcgc ccccccccgc atcaagcatc
      241 cgcaagccgc cgaggggaga cgaacagcca gacttctttg tcccgacgct ctatgacgtg
      301 ggcacaaagg attcccgcag catcatggac gtggccgtgt tccgtctttc caagaaggac
      361 cggcgagcgg gcgaggttat taactacacc ctgcccgatg gtcatgtcac cgtgtcggca
      421 ggcgcggccg gcatggcttc ggtgtgggat tacgacttgg tgttaatggc cgtgtcccac
      481 ctcacagagt caatgaaccg ctacagggaa gggaagggag cgaagcctgg ccgcgtgttc
      541 cggccgcacg ttgcggacgt gttgaagttc tgccgacgag cggacggcgg caaacagaaa
      601 gatgacctgg tggaaacctg catccggctc aacaccacgc acgttgcggt tcagcgcacc
      661 aggaaggcca agaacggccg cacggtgacg gtatccgagg gcgaggccct gattagccgc
      721 tacaaggtca tcaagagcga tagcggcaag cccgaataca tcgagatcga gctggccgat
      781 tggatgtacc gcgagatcac ggagggcaag aacccggacg tgctgaccgt tcatcccgac
      841 tacttcctga tagacccagg cattggccgt ttcgtctatc gcctggctcg ccgcgcagcg
      901 ggcaagagcg ccgcaaagtg ggccttccag acgatctacg accgcagcgg cagcgcaggc
      961 aagttcaagg agttctgccg catcttgcgc aacatcatcc aggccaacga cttgccggag
     1021 tacgagctgc gcgaggaagc cgggcagggc ggcccgctgc tggtgatgac ccatcgcgac
     1081 catgccgatg cagagctgcc accacctgcc ggtgtgtaat gtacggaggg agagggccaa
     1141 gagtccgtca aaagtttagc taaaccccgc tttgatcggt tccgaccctc tcgcctgtgg
     1201 atagcccgta ctttgcacac cgaaacccgt acattaccca ccggcttccg tactttgcac
     1261 accgaaaccc gtacattacc caccgggcac atttacaacc cattgattca tatatggaaa
     1321 actgcacttt tcctgcctaa aactctttaa aacatataaa aactcttaaa acccgcctgg
     1381 cctgtgcata agtcgcggcc agatcgaacc gcccaagcgc cctacgggcg cgcctaccct
     1441 ccgcgcttcg cgctccgccc cgccgccgta ggcggcggcc tgcgccttcg gcggggcacc
     1501 cctgcggggt tcccccggct tcgccggctc cccctccggc aatcgtgctg gcctacggcc
     1561 aggcggctta ccagggcgcg gcacgccgcg ccggtggcaa ctccaccgcc tgcgctctca
     1621 tccagcgccg tgcccgctga aaggccgcaa aaagcggttt agcagggaaa agcggcttgc
     1681 ggaggccgtg cccaacctca accgcctgcg cgagttggaa ggccgcaggt tgaacgccaa
     1741 gaaaaagggc aagcggccga gctggccagc atcgccattt ccgacgagga aggcccagaa
     1801 ccacacccag gccgcgcagg agcgcccagg agacgcgaaa ccggcgagag tagggatagg
     1861 gtgccgccga ccccggaaaa cgccgccacg gcccgcacag ggccaaattt cgaccccgac
     1921 gcgctcccgg ccgggctggc cctggcacgg tgcgtagtat ttcgccgtag ggtttgtaaa
     1981 ttgtagtatg tagtagtatc atacaacata ctacaacgct acatttcata caggagcaac
     2041 cgctatggca gtcacgaaag agcagatttt ttccgccgcc gacgagctgg ccgccggcgg
     2101 ccagaagccc acgcttgagg ccatccgcca gatcaccggc gggagctata cgacgatcag
     2161 cccggccctg aacgaatgga aggcccgcca ggtcacggcg gccgcgcccc tgcgcgagcc
     2221 tgcgccccag gccgtcgccg accgcctggc cgagctgggc agcgacctat ggacaatcgc
     2281 cctcgacctg gcgaacgccc ggcttgccgt tgagcgggaa gggttggaaa aggcccgcgc
     2341 cgagctggaa gccgacaggg ccgaggcgac cgagcttgcc gaccgcctgg ccgcccaggt
     2401 cgatgacctg caatcccgcc tggcgtccat cgaggccgcc gaagcggccg cacgcggcga
     2461 agccgacgag ctgcgcggcc aactggccac cgcccaggaa caggcgcaca cggccgaggc
     2521 ccgcgcccag gagatcgagc gccgcgccgg cgagctgcgc acggaactgg acagggcgca
     2581 ccaggacgcc gaccaggcac gcggggcgct ggccgaacag cagaaggcca gccaggccgc
     2641 cgccgctcaa ctcgaccagg tgcgcggcga gctggcgaag gtgcaggcca aggcccaggc
     2701 cgaccaggaa gcgcagcagg aacagcgcaa gcaggcggcc gccgaggcgc accgcatggc
     2761 cgaacgcctc accgccgccc aggccgagcg cgaccaggcc acgaaggcgg ccgcacaggc
     2821 ccgcgaggac gccgcccgcc tggccgggca gctcgacacg ctcaaggaac aatcggctgc
     2881 gctgctggcc cgcatcacgc cgccggcggc cgaggtgaag ccgacaagga agaagcccgg
     2941 cagcggcgag tgatcggccc cttaagtgtc aagatcagcc gccgcagcgc gaacgggcag
     3001 ggaacatacg aagaaaaggc cgggaacgct tgcgcgtttt ccggccctat attgacactt
     3061 gaggggcggc cgactttagc cgctaaaatc atttactcaa aagagtaaaa aatcaaaagc
     3121 gcctttactc ttttttgtgc cgctggcgaa acgcggccag gtcgattacg tccgccgtgc
     3181 gcctggcggc cgcctgcatc ggcgtgggcg gcttgacccc ggccggtgcc gggctggcct
     3241 tcggccggaa ctgccgccac agctccgcga agaacgacca ggcgaagatg aagggcagca
     3301 gcggcagcac caggacgaag ccgaccaggc gggccaggcc gaggccgagc ggccgcagga
     3361 aggccagcgg ccgccgcagc agatcgagca gcaggccgcc gaccagccac gccggcgcgg
     3421 ccaggacgaa caccgcagcg aacagggcag ggaacagtcg gcgcaggaac cacgccaggg
     3481 cgatgacgcc gagaaccgcc gcgatgatgc ccgccatacg tcacccctta gctatggtag
     3541 gggttcgcat cgaaccgccc ggcatattcg gcatcggcca ggtagttcag ccggtcgagc
     3601 aagtagggcc gttcgccgcg caccaggaca atcggatgct ccggccccag gcgcatgacc
     3661 tcatccggcg tcagcagctc gcggccggtg aattgctggc tggtgccggt gctcttgccc
     3721 cggttcatcg agccgccgcc gcccgatagg ccgctaccgc tgttcttgcc ggtgttttcc
     3781 gtctcaaact caatcgtggc tttgcccagg ctgtcgctga tgtatttggc cgtgtcgtag
     3841 tcgtcggtgc caaagaacga cttggcgctg ttagccagga aggtttgcca cttcggatag
     3901 gtgcccttga gctgcgacag gtcttgaatg aacacccaga aggccaggcc gtagccgcga
     3961 agcaggctca cggcatcctc aatctgcttc atgtagccga gctggccgaa ctcatcgagc
     4021 aggaaggcga cccggtgcgc gggctgcacg ttgctggacg tgatcgcggc aatcaccgag
     4081 ctgatgaaca ggcgcaggaa gcgggcattc gggccgatgc ggtttgccgg catgacaagg
     4141 taaaccgtca tggccgtggc cttgatttcc gacaggtcga agtccgagcg cgacagcgcg
     4201 gccgacactc gcgggtcatc gagaaatgcc gtattccggc gggccgtcga aagcacggaa
     4261 ccacgctctt tgtcggccat gcccatgagc gtattggccg ccctggccgg gatgccgaag
     4321 gccgccgtat cgtcggccgc cattgcgccc aacacgtcga aaaattccgc ttcccccgcc
     4381 gtcagcagcc gccgcagttc gcccaggttc cggcgttccg tgtcatccag tccggcaacg
     4441 tgcagcatca agccttgcag gaagttcttt gccgattcat cgaaatgcac ggcatcgccc
     4501 ttgctttcag cgatcaccag ggcatcggcc aggatggccg attcactcac acaatccggg
     4561 ttccacacgt cgagccggtc aagcacgttg aaggccgcgc cgccgtcgcc attcaccgcg
     4621 aacggatcga ccgaatagac cgcctggccg agcgcccggc gggcgcgcgc cgtcacggcc
     4681 gcgttttcgc ccttcacgtc cagcacaagg gccgagccgg gatagtccaa gaggttcgga
     4741 atcaccgcgc caatgccctt accggagccg gtaggggcga ccgtcacaac atggccggta
     4801 aagcggaaac gctgatcgag accggcgggc gcatcggaca cccggcccag ggcgaagccg
     4861 cccggcttgc cgggcgcgac aaggtggccg ccctggcgga tttcatcgac cgttccccac
     4921 cgtgccgagc catgcgaacc gagcggatgc gtgctgctgt tatcgaccgc agcgaccgcc
     4981 gcaccggcag ccgtgaccgg cccggagggc tgcacctggc cgcgcaggtt ttcgattgtc
     5041 ccgccgagga tgcgcagccg ctttcctgtc ggagactccg cgccgaccag gcccgcgagc
     5101 cagccgaaca cccggcccgc gttcagatac gttgccactg ttccggcgac cgtcgcggcg
     5161 aggatgaacc agcccggaat gaacggcgcg aacacgaaca gaagcaacat gccgaccgat
     5221 ggaacgagga aggccaggcc aacggccgtg ccgaccgtca cagggttctg ccgccgcttg
     5281 tgcgccagcc acgcgccgac aaagcagacc gtgaccaggt agaacaacca gccgccgaaa
     5341 gccgccttga tgcgctcgcc gaagccgccg aaatagccgc tcacggtcga gccgccgagc
     5401 tgttccatgc gttccgcgcc gcgctgctgc acttgggcgc gatactccgc aagaatcgtt
     5461 tccgcttggg ccgttccgat gcccttgccg aactcaacgc ctcgcgtcac ggactgctgc
     5521 acgacattga cgccggccgt tgccgccgcc gctggccgct cgccgtccag gcccgccaga
     5581 atccggtcgc tgaaatcgtc caagctaccg gccgaggaaa ggcccgcatt ctgcaagaac
     5641 aagcccaggt cattgcgata caagctaagg atgtttgccg cgcctggcgc gttccggttc
     5701 gccagtgcag cggcttccag ctctttcagc actggcagca attcggccca cttgtcgcgg
     5761 taggcttgcc agttcccggc attcgtcgcc atcgccaaat cctgctgccc aacatccagg
     5821 gcttgcaggc tgttttccca cttctggaat gccgcctctt gcgcatcaca aacgcgcttc
     5881 acccaggcat ccgcatcttc cgcgccgtag aacttcgcgg cctctgccgc tgggttgcat
     5941 tcagggaaaa cgcgggcgtg cgccgtgttc gccaacgaca gcacgaatgc tccaaaaaat
     6001 accgctacga cagcggccca caatctcgcc aaaaattctc taacgtccat catgcttcac
     6061 ctctatgtca ggttgaattg acggctagga acggttgccg ccgtcctctc catcatcgga
     6121 aaaagcctca tcgcctgccg cctcaaagcg tgcttttgtg gcgtcagcat ttggcccgtt
     6181 gagcgcaccg gccgcgaggg ttaaagcgcc caaaatcacg gaaaccggat agcccgccat
     6241 gccggacttg atgaccaagc cgccgagctg gattttccgg cgggcatctt ctctcctggc
     6301 gttcatccga aggcgtccta aattgcgctc aatctgcgct aggcgttgcc gccatcgggg
     6361 cagcttgctt gccttcgctt ttgcgaaatt tcgaggcgat agcctcaaac tccttccgca
     6421 gttcggcgtc ttcaatgtcg agatcgccga ggccagcgga gagggcaatc ttgccgatgc
     6481 gctcagccgc tttgctttcc agttcggcca gttctgcctt gatgcggttc agttcggcca
     6541 gcttgtcggc cttgcgttcg gataacgtct tgcgtgccat cgtcatagct cctttcgaga
     6601 ttgtgggtta tgcgcacacg atcttaccac cggcaaggct ggcggggaag gcgcggcgct
     6661 ggtatagtca tggagcgaaa gcgaaatgcg cacttacggg ttgctgcgca accctcatgc
     6721 ggccggggac accccggcac cccttttagc ggctaaaacg ccaagggaaa aatggcgatc
     6781 taccacttga acacgcacac gataggacgg gcggccgggc atagtgccgt ggccgctgcc
     6841 gcctatcgtt ccgcgtcgtc gctggtagat gagcgcaccg gcgaagtgtt cgacttcacc
     6901 agaaaaggcg gcgtgttgtc gtccgagatc gtcacgcccg caggcgtgcc ggttcccgaa
     6961 cgcgccgccc tatggaatgc cgccgaggcc gccgagaaac gaaaagatgc gcgagtggcc
     7021 cgagagtggc gggcagccct gccgcatgag ctgaacgaag ccgaccgtaa agagctggca
     7081 acgcagatgg ggcaggccat cgccgaccgc tacggcgtgg ccgtggatgt gtacattcac
     7141 gcgccagaca aggagggcga cgaccggaat tttcacgttc atatgctggc gaccacgcga
     7201 accattggtg cggatggaac gctaggcgcg aaggccgtca ttgagctggc gaacaaagac
     7261 aggcagaagg ctggcattcc tggcacaagc cagggcgaca tcatcgagat tcgccaacag
     7321 tgggccgagc taacaaacga ggcgctagag cgggctggaa tcagcgcccg cgtagatcat
     7381 cgcagctatg cagatcaggg cgtggaactc acgccaacca agcacatcgg cagcgatgcc
     7441 gtggcgatgg ataggcgcgg cctggaagcc gaccgcatcg acattcacaa cgccgaccgg
     7501 caggagcagg cgcggcagat tgtcgagcgg ccggagatca tcctagacaa gctcacggcc
     7561 acgcaagcgg tattcacgcg ccgcgatatt gccgcagagc tgaaccgcta cattgacgat
     7621 gccgaccagt tccagggctt gcttgccagg ctggaacaat cgccgctgct tgtcgagctg
     7681 gaaccggcca gcggacgcga cccggcgaag ttctccaccc gcgaaatgat cgacaccgag
     7741 cgcggcatgg tggacagtgc cgagcgcctg gcgagaaccg gcaggcatgg cgtttctagc
     7801 cccatcacga acgcggcaat tgacggcgca ggcaccctat cggcagagca gcaaaacgcc
     7861 gttcggcacg ttctcaagcc gggcagcctg gccgtggtca tcggcgacgc cggcacaggc
     7921 aaatcgttct cgatgaaggt tgcacgcgag gcatggcaag cgcagggctt caacgtgcgc
     7981 ggcgcggccc tggcgggcaa ggcggccgac gaattgcagg cgggcagcgg catcgacagc
     8041 cgcacgcttg catcccttga atttgcttgg aagaacggaa aggacaaact cacttcccgc
     8101 gatgttctgg tgatcgacga agcgggcatg atcggcagcc gccagcttgg ccgcgtcctg
     8161 aaagccgccg agcaggccgg ggcaaaagtc gtcctgctgg gcgacgacaa gcagcttgcc
     8221 gccatcgagg cgggcgcggc tttccgtggc gtggtgcagc atgtcggcgc ggcagaaatc
     8281 accgaggttc gacgccagaa agaggcatgg gcgcgtgccg ccggccagga gcttgcgcgc
     8341 ggttctgttg ccgatggctt ggcggcctat gccgagcgcg gctacgtcaa aatccacgat
     8401 agccgggacg ccgcccgcga cagcctggcg gccgcttatg tcggcgacca gggcaagggt
     8461 agccagatca tccttgcgca cagcaataag gacgtgcagg cgctcaatga agccgtgcgc
     8521 caggcccgca aagaacgtgg cgagctgcgc ggcacggccc gctttatgac cgagcgcggc
     8581 ggccgggagt ttgccccagg cgaccgcatc gtgttcctga aaaacgaccg cgaccttggc
     8641 gtgaagaacg gcactcttgg caccgtcgag cattcggaag atggaagcct ggccgtgcgc
     8701 ctggacagcg gcgaagcccg ccggttccag gccgcgcaat atgccgccgt cgatcatggt
     8761 tacgccgtca ccatccacaa ggcccagggc gtcacggtcg atagggccta cctgctggcg
     8821 acccccggca tggatagaag ccttgcctat gtcggcatga ctcggcaccg cgaggcggcg
     8881 accttgtttg caggagccga cgacttcacc gaccgccgcg tcggccgcct ggtcgatcat
     8941 ggcgcagcgc cctacgagaa caagccggag aaccgagcca gctactttgc cactctggaa
     9001 aacgacaagg gcgaacggca caccatttgg ggcgtcgatc ttgaacgcgc cattgcggcc
     9061 agcggcgcga agcgaggcga ccgtatcggc ctggaacatg gcggctctga aaccgtccgc
     9121 ctgccagacg gcaccacggc cgagcgcaac acctggcacg ttcgcggcgc ggccgagctg
     9181 gccgccggca agctggccca ggtgctaggc cgtgagcgtc cgaaggaaag cactttggac
     9241 tttgccgacc ggcgcggctt cgacggcgag agcgtcatgc gccgttggct ggaacgcggc
     9301 cgggcgaagg tggccgagct ggccgggaag atgcgcgacg gcctgcgccg caacctggaa
     9361 cgccacggcc gccccgacct catgcccgcg accgacattg ccggcacgcc gaccgtgcag
     9421 cggccgcagc agatcgagcg cacgccagag gacgagaaga agcgccgggc ggccgagatc
     9481 gcggcgaagt tccggcagca ggtcagcgag gaacgcggcg agcaggtagc gccgcagcag
     9541 cggccccagg ccgaacgccc ggcacccgac ccgctggccg gattccgcgc cggtgtcgag
     9601 cgtgccgagg cggcaggcgg cggcctggcc ctggacgtgg cgaaggcaca gcttgccgtc
     9661 gcccaggagt tccacgcggc cgggaaagac ccccggcacc attcggcggc catcatgcag
     9721 gaaggccagc agcgggcctt tgcgggcctg gcgcagccgt cccaggccaa ggccgagccg
     9781 gagcgcccgg cggccgtcca ggtcagcgaa caagaccgca agcgggccga ggcgcacgcc
     9841 aagatggaag tcagccagtt ccgggcgctg gcggtgaagc ggcaggcagg ctttgcgggc
     9901 tactccgacc gcaacccgag cgcctggcgc gagctgccgg acgagctgcg cgggcgcatc
     9961 gagaggttca acgcaatgcc caaggagcgc caggccgtcg agctggacaa gatgcagcgc
    10021 gagcttgccg acaggtatgc acgcaatccg caggaaatca cgcgcagccg acagcggcag
    10081 cgtgagcagg aacgcggccg cgatggtttc agcagatgag aaaaatgttt ttactcattt
    10141 gctcattgtg atatgatgca atgagtaaat gatattttat gttttacgcc aggaggaacg
    10201 acgatgagca gcaccgaacc gacgaacgaa gaacttgcca agggctacgc caagcagatc
    10261 gaacaggccc aggagcgccg cgaggcgaaa gaccttgagc gcctggaacg ccacggccgg
    10321 gcggccgcgc agcaatccca ggctatcgaa gccgagcagt cgggccaggg cgtcgagctg
    10381 gacgcaagcc agaaagtcga gctgaacgag atcaacggcc agcaggcaaa ggcgctggca
    10441 aaggaattga accgggacaa cgagaacggc gaccaggtga agcaagccag ggcgcgggag
    10501 atcgcagaac ggttcaagga gaaggcggcg aaagatcggg agcagtcccg cagtttcagc
    10561 cgctaaaaag cggtgcccag gaaggcggca acccacctgg acacctgacc acaacccacc
    10621 tgacatagag gtgaaatcat ggctacttgc aatgttactg gcggcacccg ccgcgaccta
    10681 acgaaatatc ggattacctt tgccgtttcg ctggccgtcc tgatcggcac tggcggcatg
    10741 ttctggcacg cctaccggac gcacgccggt tggctcaatt ccggtgatgt cgccgtgcgc
    10801 gatgccatca tggacggcac tatccagccg ccggccgatg gcaagggggg cgcacgatga
    10861 aggcgcagac ttggcgccgc acagccctgg cggcctacat cctggcgggc gcatccctga
    10921 ccgtggccgg ctacaccgtc gccacgaccg aggcgaacgc aaacgccctt taccgcttcg
    10981 ccgaacatga caacgacgcc tattcagccc ggagccggag cattgcggaa ggcatcaccg
    11041 ccgccgcctt gcgcggccag ggcgacggcg tgccggatgc cgccgactgg ccgcgcatca
    11101 ttccccaggt catcccgtcc gccgtgaacg tggccgccct gccctttgtc accaggccgg
    11161 acgatgaaag ccgcgtctgg cttgttcctg gcgttcaatc caacccggcg acggccgccc
    11221 ttggcgctgt ccgcttctgg ctgaaaagcc gcgaccagga gccgcgccac tggcagacca
    11281 tcggcagcgg cgcgtttatc gcagatggcc ggtatgtcct caccgccgcc cacgtcatcg
    11341 acggcaagag cggctatcgc gtgcagacgg ccgccgggcg ctggctggat gccgagctga
    11401 tcggccttga cctacggcgc gacgtgggcg tgttgcgcgt cagcgaaccc ggccagccga
    11461 tcaagacggg agccatgccg attcagggcc agccggtatt gaccatcggc gcacctggcg
    11521 gccgagggtt cagcgtgagc gtgggcattg tgagccggta tgcagaaagc gcccggtttt
    11581 ccgctgatga agccttgcag atcgacgcgc ccgtgactgg cggcaactcg ggcgggcttg
    11641 tcgtcagcgc gagaggtgaa gctgtcggcg tggtcagcca ttccgatgcc gcctttaccc
    11701 aggccgtgcc gatcggcacg gcgctggccg tggcaacaga gctgatcgag cgcagccggt
    11761 aagcctgcca ggaactcgaa ggccgggcgc ttgcgcccag gtcgataccg ggcgccgccg
    11821 gcgttccgat gaatccccaa cctttggagc aacagctatg aaacttcgca attccttccc
    11881 gctggcggcc gtggccgccc ttctgatggc ttcgaccgcg cacgccggcc aggatgccga
    11941 tgcgtcccgc ttttcgatca tggcccccgt ccaggcggct tatgacgcct accaggtcac
    12001 ggcgagccgg gcgcagaaca caatggacag catcgaggcc gagctacgcg aacctggctt
    12061 gtcggccgag cggcgcgagc tgctggcggt atcgcttgcc acccttcgcg cccgcgaggc
    12121 ggccgcattg gagaggctgc acgctgaatc ggccctggcg cagttgaaga tggcagactg
    12181 gaacgcgagc cggtaacggc gaccaggaac aagaagggcg gccagtggcc gcccttttca
    12241 cgtctggatc ttgcatcgtc acttctggcg atgagatcca ccgtcgcggc cgtcatcgtc
    12301 gggactggcg atgttggcca gcccctcgcc ttccagggtt tcgcaggccg gccactcgcg
    12361 gccaacggcg ggcgcatcga cccattcgcg ttcttcgggc ggtagcggct gtgaatagtc
    12421 ggattcggcc agcagctcat cgagcgtgta gcgcgggcgg ctcatcggcg ggcctcatcg
    12481 agccaggcca gtgtttcggc gtcatgggca gctacacggg ccaccagctc gcgcaccagg
    12541 gcggccaggc tcatgccgtg cgctaccgcg acggcggcag cttcggcttt cacgttcgca
    12601 ggaacggcgg cgctgatggt ggcggcttta ttggtcattg gcgaaactcg atttttaggc
    12661 ttgggtcggt ttcgacttct tccacgatct cgttatagcg cgttaccacg tcccccacgg
    12721 ccacgcgccg gccttcggcc gcgaacttgc ccagctcgta gaggttcaag gctatgaact
    12781 gctcaacctc gaaaacgtcg atgcgttcgc ccaagccggc attttcggcc agcccttcgg
    12841 caaccgtcag ccctcgtcct gtcgtgacga tgattggccg gtggccgtcg tcgatgttgt
    12901 cgcggcaccg accaatgaca gcttcgccgg gtgccgtggt gacgtggata gccacatcac
    12961 caatgaaaaa atcgccattg cggcccgttt gagcgtcaga cgttgaaaag ctgttgtgat
    13021 cgaagttgcc ggcacccagg gcgcaatcga gcttggcccc taccaggtgt tgcagcaccg
    13081 cgccggcata ctgcatcccc ggcgtgttgc gctgtcgttc ttcggcttgg gcgatcatgt
    13141 cgcgcaccag cgttcgcaag ctgcgcgagg cgtccaggcg aatcttgaag ggcttggcgg
    13201 aaaagaactc atgcacgcgc tcaatccaga acgcttcaac ggcatcgaga tcgaccgtgc
    13261 cgccggcggc caggccgttg aggaaggcca catattcgcg catgttgctg atgctgccgc
    13321 gactggtgcg gccgccttca gcggctaaaa cgcgggtaat gtcgtggcgg ttcagaaccg
    13381 cttgcaccgc gccacggccc aggccgagaa cctggccgcc ggcttcggtc agcagctcgt
    13441 cggggttgag cggcagaccc tttcgggcgt gctgcgtgac gaccaacgca acgcacagcg
    13501 ggcctttgcg gttgaatttc cgatctttgt tgaagtcccg cagggccgtt accaagtccg
    13561 tcattacgcc gccttcttct cgaccgccaa ggccgccgct agcagcggtt ctaggatatg
    13621 tgcggccagg aagcgcacga ccggcacggc cacgccatcg ccggccaggt gataagcatc
    13681 gttgtagttc ttgggcagcg cgtaggtgtc cggcaagccc atgaggcggg ccgcttcgcg
    13741 cggcgacagc agacgcgaac gaacgcgctt gccttcaatc accatgatgg tttgacggct
    13801 tgaaccgccg gtaggcgtgc gcaggcaacc ggccacgtca tcgaaacgga tttcggcgcg
    13861 ttgcaccttc tcgccgttcg ggccatcggc gcgggtgcgc ttgtagatcg tgccgacctg
    13921 gcggccgccg gctttcttgg cggcttcaac tttcgccagg tttagcgggt tcatcatgcc
    13981 gagcagcttc tttgtttcgg cggccgtgtg ccaggtgacg ccgtgcggct tatcttccac
    14041 taggtcggca aagatcgacg tgcgggccgg aggcgccggc aggcgccacc attcccatgc
    14101 gctttgcgca cgcttcgaga gcttgccgta agcctcgacc agcgcggacg gatgccattc
    14161 cccttccggg ccgctggcga ccagggcgcg aggaatcggc gaggatttgc gcacgccgat
    14221 gatgaacaga cgtgggcggg actgcggcag gaaatgaacg gcgttgatga ccacggcacc
    14281 gaaccgatag ccgttgttcg acagggccga gctgatcgcg gcgaagtcct tgccatcatg
    14341 cgagctaagg gcgccgcaca cgttttccag caccaccagg cgcggcgctc gatcttcatc
    14401 ccccagggct ttgatgagct gccagaacgg ccagaaagtg ccggagcggt cgcccttgag
    14461 gccagcgccg gcaccggcca gggaaaggtc ttggcaaggg aacgaggccc aagccaggtc
    14521 agcgccggcc ggcaggtcgg ccgtggtcag cgcggccacg tcgccgacgc gcagatgatc
    14581 ggcgccccaa ttcgcggcgt agctggccgc cttcttcggg tcgaagtcgt tggcgagcag
    14641 gcattgccag tcggggccaa ggccggcgcg ggccatgccg ccgccggcga aaaattcata
    14701 gaaattagcc attggctccc cctgcacctt gcgggtgcga tgacgaagcg agacgcccca
    14761 ggcgttccgt cacggcttcg gtaatccaag tgttgcgcga tacgctgccg gcccggcgcg
    14821 agcattcggc atcaatggct ttcagcacct cgggagtgag gcgcagattg atacgttcca
    14881 gcgcactaga gcgcgaatct ttgcggtcgg ctttcatggg cgccattgtg gcgccatctt
    14941 ggctcaaggc caaggaattt attgtaagcg tcgcataagt cctaagtttt gcgacacgcc
    15001 ctgcattgtc attttcagaa gacgactgca ccagttgatt gggcgtaatg gctgttgtgc
    15061 agccagctcc tgacagttca atatcagaag tgatctgcac caatctcgac tatgctcaat
    15121 actcgtgtgc accaaagcga ggtgagcatg gcgacggaca ccccacggat tccagaacaa
    15181 ggcgtggcca ctctgcctga tgaggcttgg gagcgtgcgc gccgtcgtgc ggagatcatc
    15241 agtccgttgg cgcagtcgga gacggtcggg cacgaagcgg ccgatatggc ggctcaggcg
    15301 ctgggcttgt ctcggcgcca ggtatacgtt ctgatccggc gtgcccggca aggcagcggc
    15361 ctcgtgacgg atctggtgcc cggccagtcc ggtggaggta aaggtaaggg gcgcttgccg
    15421 gaaccggtcg agcgcgtcat ccacgagcta ctgcaaaagc ggttcctgac caagcagaag
    15481 cgcagcctag cggcctttca ccgcgaagtc actcaggtgt gcaaggctca aaaactgcga
    15541 gtgccggcgc gcaacgctaa gttcagcggc agtttgtaag ttgcgcgttg tggaatactt
    15601 tgcgaagcaa accacaaaag cgcaacttac aaactgtcca gccacgtagt ggcgtgctgc
    15661 aacgacttgt tagaaattta gttgcttggt tttgatggtt ttttactttc gtttaaccct
    15721 ttaaccgcct gctctaatgt aagtttcaag agtgatgcgt ctccaacttc actgtgactt
    15781 ggaacaacca gttttgcctt accatatttg gactttaata atttggcgga ctttggccaa
    15841 gcttctatat ttgcgtcacc caaattgcct aaaccgtacg gtttaataaa acaaccaccg
    15901 aataatattt tcctttcagg cagccaaacc actacgttat ctggagtgtg tcccgggcct
    15961 ggataaaaaa cttcaatttt atttttaact agccaatagt taactccgct aaatgaattt
    16021 gtggcttgaa ccttgccgtc ttttttaagc agttcatttg ttaattcaga tgcatacgtg
    16081 gggatagatc gagaattaag ccactctatt ccgcccgtgc tgtcgctatg aaaatgagag
    16141 gaaatactgc cttttatttt atagccacgc tccacaaacc aagtgactaa cttttcagta
    16201 tctttagccg taaatggagt gtcaattaga taagcctcag catttacaag aaccaccaaa
    16261 ccatgtttag gaacaacgcc ccacccgtta acttcttcaa acgaagtatg aacataaacg
    16321 ccttcatcaa gcttttcaat ttttaaatct ggcaaagact ctgctgcggt agcaatgctg
    16381 caaaacaaaa atataaagaa tacagataac ttgctcatac ttttcctttt ctaacgcctg
    16441 agttaagccg acttgcgtag tggagccaaa gccatggcaa gctctttctg ccatggcttt
    16501 ggtgtaacga agcaagttcg gcttgaacga attgttaggc accaacgcgg cgcccctcac
    16561 aacggtttcc ggaagcagcg tacttgagca acctccgtga atccagccgc caggtgcgcc
    16621 gaggtgcttg cgctgttggt aagttgagtg tcggaggcga attcggtgca tccgcgacca
    16681 cgagcccaat gctcggcggc ttttaccaga gctacaccta cgccctgacg ccgcgcactt
    16741 ggcacaacgt accaaccctc caagaacgca acgttgccgg agtagcactc ctccgcatac
    16801 gggcggatcg aaagctcggc aaaccctagt gcttctccgt cgggtgctac agcaatgagg
    16861 acagcagcag gccgggcgac ttttccggac agaaattctg cgatctctga ctggtgctct
    16921 tgacatgtgc catctggcca cagctcacag cgcatgctta gccaactggc cgaatcggta
    16981 gtttcaacag gcctgacgag cggcgaacta tccattggtg cctaactttg ttttagggcg
    17041 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
    17101 acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc
    17161 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
    17221 gcgtgagcgc atacgctact tgcattatag cttacgaacc gaacaggctt atgtccactg
    17281 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
    17341 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg
    17401 tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg atctgccctg
    17461 gcttcaggag atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga
    17521 tgaagtggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg cccagcttct
    17581 gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg cgggtcaagg atctggattt
    17641 cgatcacggc acgatcatcg tgcgggaggg caagggctcc aaggatcggg ccttgatgtt
    17701 acccgagagc ttggcaccca gcctgcgcga gcagctgtcg cgtgcacggg catggtggct
    17761 gaaggaccag gccgagggcc gcagcggcgt tgcgcttccc gacgcccttg agcggaagta
    17821 tccgcgcgcc gggcattcct ggccgtggtt ctgggttttt gcgcagcaca cgcattcgac
    17881 cgatccacgg agcggtgtcg tgcgtcgcca tcacatgtat gaccagacct ttcagcgcgc
    17941 cttcaaacgt gccgtagaac aagcaggcat cacgaagccc gccacaccgc acaccctccg
    18001 ccactcgttc gcgacggcct tgctccgcag cggttacgac attcgaaccg tgcaggatct
    18061 gctcggccat tccgacgtct ctacgacgat gatttacacg catgtgctga aagttggcgg
    18121 tgccggagtg cgctcaccgc ttgatgcgct gccgcccctc actagtgaga ggtagggcag
    18181 cgcaagtcaa tcctggcgga ttcactaccc ctgcgcgaag gccatcggtg ccgcatcgaa
    18241 cggccggttg cggaaagtcc tccctgcgtc cgctgatggc cgattctgtt gaaaaagtag
    18301 gtttagcggc ggcctgccga tcaggtgtgc ctgctgtcga agtgactgca agccacttca
    18361 ggttgccttt cggcgtttcg ctgagcgtcc ttgctcaggc ctgagggtta atttgaaggt
    18421 ttctgctctc agcaggcgta cctatcccgt gagcggtggc ccttgaggca aaagcttggc
    18481 catgcgtcgc aggttctgca ccgccgccgc caaagtgaat tcgtcagtgg cacctgtcag
    18541 gccgcgcagt cgtaaacgat cgagtttcat gatccgtttg aggtgggcga acagcatctc
    18601 caccttcttt cgttcgcagc gagagacgag gtattccggt gtcttggcga tgcgcctggc
    18661 cacatcgcgg gcagcctcat ggatactgcg gacgatcttc cgattcggcg tgttggggca
    18721 gcatttcgct ttcagcggac aggtagtgca gtcggtttgg ctggagcgat aaatgatggt
    18781 gtgggccttg gtcacccgcg atcttttctg ggtgaaggcg cgccactcac tgcgtagtgg
    18841 tttgccggct gggcagcggt attcattggc ctcctggttc cagtggaagt cgttactgga
    18901 gaggctgtcg tccttgcgct cagtcttgtc ccacaccggc acatgcggct cgatgtcctt
    18961 ttcttcgacc atccaggcca gcatcggggc ggtgccataa gcggtatcgc cgataaggcg
    19021 ttccggtgtg agatcgaact gagctttgac acgctcaacc atcgtcctag tcgaatcgac
    19081 ttcggcggta cggtgcgccg gggtagcttc cacgtccatg atcacaccgt gctcagtgtc
    19141 gatcaggtaa ttcgtggagt aggcaaaaaa ggccggccca cctggcgctg ctgtccaacg
    19201 ggactgagga tctgtgagcg aaattttctt gggaagagcc tcagccagcg cctcttcatc
    19261 aagggcttcg aggtactcgc gcactgcgcg gctgctgagc tttggatcgt tccaatcgac
    19321 ctcatctccc gctaccccac gttgccggct ggcatccgcc ttgatgatgc tagcgtcgac
    19381 ggcaaaacct tcgcccttga ctaggccggc tgccatgcaa cgccgcagca cctcattgaa
    19441 caaccagcgg aacagatcgc tgtcacgaaa acgcccatgg cgattcttcg agaaggtcga
    19501 gtgattgggg acttcgtctt ccagacccag ccggcagaac cagcgatagg ccaggttcag
    19561 gtgcacctct tcgcacaatc gccgctcgga acgaatgcca tagcagtagc cgacgaccag
    19621 catgcgcacc atcaattccg ggtcaatcga ggggcgtccg atgggactat agaagtccgc
    19681 caggtaggcg cgcagatcac taagatccag gcactggtcg atgctgcgca ggaggtgttg
    19741 agccgggacg tgatcttcta ggttgaacga gtagaacagg cgctgctgtc ctcccggtaa
    19801 ctgccccatc atgctgttcg cccccacgct cgctgacaga gcaattttgc caacggtatg
    19861 gagaggccgc tactttttca acagaatcgg ccggcagcag cccgtcgttg cctgatggat
    19921 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
    19981 aaacgacatt tgaaccggga accttccctt tttggctagg cgtgaaacaa tggtcatttt
    20041 ccctataccc aaaatgacca ggaggcccgc atgaaagtcg cacgcatcta cctgcgcgtg
    20101 atgtcatttt cagaagacga ctgcacggaa tatcaggagt cggctgcact ggtggattgc
    20161 agcgcggtgt acagggccgt cttgctcacc ttgagccgtg tagcggcctc tcggacatta
    20221 agcccgttgg cgatgtgctc ccgcgctcgc tgcaacttgt cggcggtgac aaccggcttt
    20281 cgcccgccct tcctcccacg agcggcggcg gcagtcaacc cggccttggt gcgctcgcgg
    20341 atcaagtcgc gctcgaactg gcccagcgcg ccgaacacgt ggaagatgag ccgcccgcct
    20401 ggcgtggtgg tgtcgatggc ttccgtcaga gaacggaagc cgacgcctcg cgcttccagc
    20461 gcgcctatcg tttcgattag gtgcggcata gagcgcccga gccgatccag ccgccagacg
    20521 gccagcacgt cgccgtcgcg caggtaggcc agcgcatcag ccaagccggg gcggtcggcc
    20581 ttggccccgg aagccgtgtc ctcgaaaacg cgctcgcagc ctgccttgcg tagcgcatcc
    20641 gtctgcaagg cggtgtcctg ttccgccgtc gatacccgcg catagccgat cagtgccatt
    20701 tgccgcctct cttgtccgtc attccgtccg cctatcttaa tgtccgacaa cccgttgtgc
    20761 aataactttg ctggacagtg tccggcatgg ccgactaatg atcgtttgac ggacattgac
    20821 ggtaaggcat ttttgcatta ctatgtgaag catcaacctt gattgcgagg gcaaaccacc
    20881 atgaccacat tgaccgttac cgcacgggga caagtgacgt ttcggaagga cgtactgcaa
    20941 cacctcggca tcaggccagg cgacaagatc gagctggact tgttgccaga cggtcggggc
    21001 gtgctcaagg cggcacggcc cgcagggacg atagccagct ttgtcggcct gctcgcgggc
    21061 aagacgcaga aggttgccac catcgaagaa atcaacgagg cggcggcgca aggctgggca
    21121 ggtaagcaat gaaggtcgca gtcgatacca acgtccttgt gcgtgcggtt gtgcgtgacg
    21181 atcccgcaca agcggacgtt gccgccgcag tcttgaccga cgccgagttg atcgcggtcg
    21241 cgctgccgtg cctatgcgaa tttgtttggg tgctgctgcg tgtctacggc ttccagcaag
    21301 ccgacgcggc cagcgcgatc cgggcactac tggccgccgc gaatgtggaa gtgaaccggc
    21361 ctgccgtgga ggctggcttg ctggtgctcg acgcgggcgg agactttgcc gatggcgtca
    21421 ttgcctacga aggcaactgg cttggcgggg aaaccttcgt ttccttcgat aagaaggcgg
    21481 tggcacttct cacggcgcaa gggcaatcaa cgcgcctttt gtgacggaca tgagcatgaa
    21541 cgaattccgc cgactggccg cgaagatcga ccaacacatg cagcagcttg ccgcccaggg
    21601 tgtcagcgag gcccatgcca tcatcaatcg catgatgggg tacgggcctg acctgcacag
    21661 gatttgggtc ggcacatccg atcagcagct catggcgctg tcccgcgagt tcccagggtt
    21721 ctaccgctac gcccgcatca tggaagaggc atccgaagcc gagcgccgca aggcttcgcg
    21781 gccctatgac ggcatggccg agttctccga acagcacaag caaatgggcg cgcaactgct
    21841 gaccacggcg gccacgcttg agcgtggcta ccaggcgttt cgtgcgagcg gcagtctcca
    21901 agacttccgg cctcagctcg acgagctggg ccgcttgcat cggcaatggc tgtccgacct
    21961 ggaagccttc aaagactcgc tgcgcacgca gggcgcagaa cccaaggtgc tggaatacgt
    22021 gaacgaggct ttcggccgcc tggccgagcg catcaagcag cttgccggtt gatcgccgga
    22081 attttcggcg gttccggctt tgtcgcgccg tgcacaagag ttctgagcaa ttccgatctc
    22141 tcgcggcgct tcgtgcatag aacttctgac cgttcccggt cagaagttct atgcacacac
    22201 ggcggccagc ccagtgaact ggtgcagtcg ccttctgaaa acgacatgca tccccctttt
    22261 tcctggcgtc atcatgttgc aaaagtcggt cgcaaatagt ttattatttg cgacgatatt
    22321 tgcaacgaag ggcgcgccat gaaagacaag ccgatgatcg gttacgccag ggtatcgacc
    22381 gacgaccagg atttgacgaa ccaacgcgca gagctgcacg ccgccggctg cacgcgcatc
    22441 tttgccgaga agatcaccgg cgcccatgcc aagcggccgg agctaggccg gatgcttgac
    22501 cacctacgcg ccggcgatgt ggtgacggtc acgcggctgg acaggctggc ccgtagcacc
    22561 cgcgacctgc tggacattgc cgagcagttg caggcgaaag gcgcgggcct gcgctcgctg
    22621 gccgaaccgt gggccgacac caccaccccg gccgggcgca tggtgctgac cgtgtttgcc
    22681 ggcatcgccg agttcgagcg ttccctaatc atcgaccgca ccaggaacgg ccgcgaggcg
    22741 gccaaggcgc gaggcgtgca gttcggccgc cgccctaccc tcacccccgg ccaggtcgaa
    22801 catgcccgcg agctgctggg cgctggccgc acggcggccg aaacggccga gctgctgggc
    22861 gtgcatcggg caacccttta ccgggcgctg cgcggccagg aaaccagccg ggccgaggcc
    22921 cgccggcgcg gcgcgtttgt agaggacgcc ttgaccgagg ccgatgcgct ggcggccgcc
    22981 gaccaggagg gcatgaaggc atgagaaacg gacactaccc cctgcccccg ctccggccgg
    23041 agctgcaagc cgcgcacgac gagatggacg ccaagggcga ttactttgcc tatgccgtgt
    23101 gccgaaccca tgacggccag gcgtgggaag tgacgaccag gcagggcggc atgtatggcg
    23161 cgctcgatgg ccgctatctc gaccacgacg aagcggccgc cgccggcgtg gcctggctgc
    23221 tggaacagct cgaccgcgaa ccgacagcag acgaggcaac gtatcgggcg ctatgggaaa
    23281 gtatgggcaa atgatgaaaa atgcttttac tcattcgcgt gtcgtgctat gatgcaatga
    23341 gtaaattatc atttgcatga ccaccaggag agcgcatgaa agtcattgcc gtgctgaacc
    23401 agaagggcgg ttcaggaaag accaccatcg ccacgcacct agcccgcgcc ctgcaactcg
    23461 atggcgcgga cgtgttgctt gtcgattccg acccccaggg cagcgcccgc gattgggctg
    23521 ccgtgcggga agatcaaccg ctaaccgtgg tcggcatcga ccgaccaacc attgaccggg
    23581 acgtgaagaa cgtggcccgc aaagtggatt tcgtcgtgat cgacggagcg ccccaggcgg
    23641 ccgacctggc cgtgtcggcc atcaaggcgg ccgacttcgt gctgattccg gtgcagccat
    23701 cgccctatga catttgggcc acggccgacc tggtggagct ggtcaagcag cgcatcgaag
    23761 tcacggacgg gaagctgcaa gcggctttcg tggtgtccag ggcgatcaag ggcacccgca
    23821 ttggcggcga ggttgccgac gcgctggccg gctacgcgct gcccattctt gatgcccgca
    23881 tcacgcagcg cgtgagctat cccggcaccg ccgccgctgg cacgaccgtt cttgaaaccg
    23941 agccggacgg cgatgcagcg gccgaggttc gtgccctggc cgccgagatc aaacagaaac
    24001 tcaattgagc ttttgaggaa atgaaaaaat gagcaagccc acaaccacac tcaccgccgg
    24061 ccgcccgagc gcacgcaccg acaaggcgcg ggccgcgacc cttgccagcc tggccgacga
    24121 cgccggcggc atgaagcgcg tcaatttcga gctacccgcc gaacagcacg cgaagctgaa
    24181 aatctacgcc acgaagcagg gcaagagcat caaagatgtg ctgtccgagt atgtcgccag
    24241 cctgccggat tgacgctcaa aaacatattt actcatttga attttagcag ctaaaggggc
    24301 ggcacc
//
