LOCUS       pPNAP08                 6459 bp    DNA     circular     30-NOV-2020
DEFINITION  Polaromonas naphthalenivorans CJ2.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Polaromonas naphthalenivorans CJ2
  ORGANISM  Polaromonas naphthalenivorans CJ2
            Unclassified.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of pPNAP08
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Polaromonas naphthalenivorans CJ2"
                     /mol_type="genomic DNA"
     mobile_element  1..6459
                     /gene="pPNAP08"
                     /product="Plasmid: IncQ-4pPNAP08"
     CDS             complement(1..834)
                     /gene="repC"
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein C"
                     /translation="MKPKQNLDHIRHEPGHVLAPGLFRSLKKGDYKRLKLDIAYEFGK
                     GERLEFKGAEPLGVPELRILQGLIGMAGPNGLLLTPEPKTESGQQLRLLLETKFDAIE
                     TNALVVKGSYRALAKTIGYADIENTKNIQESIERLWGVSVIARKGGKHMGFRLLSEYA
                     SDDADGRLYVALNPVLARAILGEAQHCRISLDEVRALKTDPARLMHQRLCGWIDIGKS
                     GKTTIETLCGYIWLDEATPEAMKKRKQAVRRALKEMEALGWKVTEYINGKFEITRPEI
                     G"
     CDS             complement(824..1663)
                     /gene="repA"
                     /codon_start=1
                     /transl_table=11
                     /product="Regulatory protein A"
                     /translation="MTIDILHAFEHQPPPLDFVLPGLLAGTVGSLVSPGGAGKSMLAM
                     ELAILVTCGQDIAGITDGKKYPCGDVAFLAAEDPAVAIQQRLYALGKQIPDPDIRMHV
                     AERLAIEPLLGKQPDIANPEWAAYIKAMAEGKRLMFLDTLRRFHSKEENDSGQMAEVL
                     GRMEAITAATGCAIVFLHHSSKAAAMNGQGDQQQASRGSSVLVDNIRWQMFLAGMNAA
                     EAKKFGVDEELKGYWVRDGVSKQNYGPPYGERWLRRGEGGVLVQGKLEAKPPAKKGGR
                     NEA"
     CDS             complement(1945..4431)
                     /gene="mbeA"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase MbeA"
                     /translation="MHIKFLPHGTGVGQDAIDYLLGEEDHKGEIRASVEVWRGNPQLT
                     GKLIDSLKTVHRYTSGVINFSPEDAPTDEEIGIVLDEFERTAFSGLERDQYDFCAVVH
                     RESNGGIHIHTIIPRVELTTGKAMNIAPPGWEYTFDPLRDAFNHEKGWARPDDPSRAR
                     LVQQGKAKPEWKSGADKKIVVTDYLTKLVANNVVTDRQGVLKALGELGEITRTTDKSV
                     SIKLEGTNKAIKLQGLLYASDFSPRTIRKVAEEAASRPAGRGRPDEAAARAARQELEN
                     AVQRRAGYNRERYPAPRPRTDLSAPSDAAAAPLDAALADPGQRLPGVVDDGQRQPVEL
                     VDGQPGTTESDELRADPATADGSVLQESSRAEAVQISEPVSEQVKIKKAAYGTVFEPG
                     FKQFAERPSVEPAAGIGGLPDVSSLLVATQWPGASKFLSGAARHNLVRHGGPGAGSVR
                     WERGSDRPTEGEGGTINDRARKIADEAIDQAKRAARATAGALGRCADAAGRAIDAIGR
                     ACRKADAAAPVIKANMDAELDRFKSDISLSDYAQAEFGYELVKKESSASSKVLKVGGD
                     KIIVTRQKDGHDVYFSTGDASDCGSIVDFLQNRKRVNLGEVRKELRQWLPSSKKPSVK
                     RPQRPPERAVATSKDRTEVLRQWATMKPYSASYLTDERRLDPQIIEAFGVRQDSHGNA
                     CIAHRDASGVLGWESKNKGFTGFAAGGTRNVSFTRLNDGPITRLVLTEAAIDAMSFAQ
                     LRHEPGTAYMSTGGTQLSQAQREQIAQIMGKYAVPVVLAMDKDEAGEKMAREIAAMAP
                     ESVQTLREVPEGAKDWNEALQAKQRAMPKP"
     CDS             complement(4434..4805)
                     /gene="mobC"
                     /codon_start=1
                     /transl_table=11
                     /product="Bacterial mobilisation protein"
                     /translation="MPKYPAREPLVLLPCRLPASLVARFAERAREQGVTLSDVLRAHL
                     TVDEAKPLGKAVPRKRAGLAKVSGADPKLLRQLVGMGNNLNQIAKLANIASKTAQPMA
                     MVELLITLKGIEKSLEKLSES"
     CDS             complement(5084..5539)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MYIREQGKKVQLLRSPYDPEKKRCVQKLAHSFPRSYSYSSTELD
                     TYLSAEQIADLSDDEKKELTEWLTVRSDKNLAESRKSSITMTPYYADQLADNISSDVV
                     DLSEKQADKIWDAMDKLAKAMRKAGYKKPAVKAAPGAQAGSADQPSLTL"
     repeat_region   6383..6447
                     /gene="iterons"
                     /product="Binding sites for replication initiator protein"
BASE COUNT     1318 a   1777 c   1590 g   1774 t
ORIGIN
        1 ttatccgatt tctggtcgtg taatctcgaa tttcccattg atatattccg tcactttcca
       61 gcccagagcc tccatctctt tcagtgcgcg cctcactgct tgtttgcgct ttttcatggc
      121 ttctggggtg gcttcatcga gccagatgta cccgcaaagc gtctcaatcg tcgtcttgcc
      181 ggactttcct atgtcgatcc agccgcagag tctttgatgc atcaatcgcg ctggatcggt
      241 ctttaaggcc cttacctcgt ccaggctgat cctgcaatgc tgggcctcgc ccaagatcgc
      301 cctggccagc acaggattca gtgcaacata caaccggccg tccgcgtcgt cggacgcgta
      361 ttcagaaagc aagcgaaacc ccatgtgttt tccgcccttt cgtgcgatga cagatacccc
      421 ccaaagacgt tcgattgatt cctgaatgtt tttggtgttt tcaatatctg cgtagccaat
      481 ggtcttggcc agcgcccggt aagacccttt aaccaccaat gcgtttgtct cgatcgcgtc
      541 aaatttggtt tccagtagca aacgcaattg ctggccggat tcggttttcg gctctggcgt
      601 cagcagtagg ccattgggtc cagccatgcc aatcagtcct tgcaaaatgc gcagttcggg
      661 cactccaagg ggttcagccc ccttgaactc aagccgctca cccttgccga attcataggc
      721 aatatcgagc ttcaaccgtt tgtaatcacc ttttttgagg ctgcgaaaca gtccaggcgc
      781 cagtacgtgc cctggctcgt gccggatatg gtcaagattt tgtttaggct tcattgcgcc
      841 cgcctttttt tgctgggggt ttggcttcga gtttgccctg caccagcacc ccgccctccc
      901 cccggcgaag ccaccgctcg ccgtagggcg gaccgtaatt ctgcttgctc acgccgtccc
      961 tgacccagta acccttcagc tcttcatcca ccccaaattt tttggcctcg gcggcgttca
     1021 tcccggccaa aaacatctgc cacctgatgt tgtccacgag taccgagctg ccgcgtgaag
     1081 cctgttgctg gtccccctgg ccgttcatcg cggccgcttt ggatgagtgg tgcagaaaaa
     1141 caattgcgca gcctgtggcg gctgtaatcg cctccatgcg ccccagcacc tcggccatct
     1201 gccccgagtc gttttcttct ttcgagtgaa agcgcctaag cgtgtcgaga aacatcaggc
     1261 gcttgccctc tgccatggcc ttgatgtacg ccgcccactc agggtttgca atgtcgggct
     1321 gcttgccaag caatggctca atggccagcc gctcggccac gtgcatgcgg atgtctggat
     1381 cggggatttg cttgccaaga gcgtacaggc gctgctgtat cgcaactgcg ggatcctccg
     1441 cagcgagaaa tgcaacgtca ccacaaggat attttttccc gtcagtaatg cccgcgatgt
     1501 cttggccgca ggtcaccaga atcgccaatt ccatcgcaag catggacttg cccgctccac
     1561 caggcgaaac cagcgaaccc acggtaccgg ccagcaggcc gggaaggaca aaatccagcg
     1621 gtggtggctg atgttcgaat gcgtgcagaa tatcaatcgt catgactcgc tctctctctt
     1681 tacagttgaa atttggttga gttatggcag tcccgcggct cgccacaagc cgcgggacac
     1741 ccctattctt tgtcgttgca gcgatcgatg gccgcttgca gtcttcccgc tgtaacccgt
     1801 ccagcgcgtt ccatcacctg atcctgttgt tcagcgctca ggtctttcca ccactcgggc
     1861 atgggggtca aaagcgttga ctcttctcca gcgaatgcct cgacaaattc aagaatttga
     1921 gcatcagtaa tcatcggttc ctccttatgg tttgggcatt gcccgttgtt tggcttgtag
     1981 cgcttcgttc cagtctttgg caccctcagg gacctccctg agggtttgaa cgctctcagg
     2041 ggccattgca gcgatttctc gggccatctt ttcgccggcc tcatccttgt ccattgccag
     2101 cacaacaggg acagcgtact tgcccatgat ctgcgcgatc tgctcgcgct gcgcctggct
     2161 cagctgcgtc ccacctgtgc tcatgtacgc cgtgcctggc tcgtgcctga gctgcgcgaa
     2221 gctcatggca tcgatcgccg cttcggtcaa caccagcctg gtgattggcc catcgttcag
     2281 cctggtaaag ctcacattgc gcgtgccgcc cgctgcaaag cccgtgaagc ctttattttt
     2341 ggattcccag cccagcaccc cacttgcatc gcgatgagca atgcaggcgt tcccatggct
     2401 gtcctggcga acgccgaacg cctcaatgat ttgcggatcg aggcggcgct cgtccgtcag
     2461 ataggacgcg ctgtaaggct tcatcgtggc ccactggcgc agtacctcag tccgatcctt
     2521 tgaagtcgcc acagccctct ctggcggcct ctggggccgc ttgactgaag gcttcttgga
     2581 gctaggcagc cactgcctca attccttccg cacctcgcct aagttgaccc ttttgcggtt
     2641 ttgcaaaaag tccacgatcg agccgcagtc gctggcgtcg ccagtgctga aatacacatc
     2701 gtggccatcc ttttggcgcg taacaataat tttgtcgccg cccaccttca gcaccttgga
     2761 gctggcgctg ctctcttttt tcaccagctc ataaccgaac tcggcttgcg cgtagtcgga
     2821 taaagaaata tccgatttaa agcggtctag ttctgcgtcc atgttggcct ttatgactgg
     2881 agcagctgcg tcagcttttc tacacgctct gccaattgcg tcaattgctc ggcctgctgc
     2941 gtctgcacac cggccaagcg ctccagctgt tgcgcgtgct gctcgcttag cctggtcaat
     3001 tgcttcgtca gcaatctttc tagctctgtc atttatcgtt cctccttcgc cttcagttgg
     3061 gcgatcgctt cctctctccc atcgcactga tccggcgcca ggtcctccgt gtctgaccaa
     3121 gttatgacgt gcagcacccg acaaaaattt cgaagctcca ggccattgcg tagccacaag
     3181 gaggctggac acgtccggca agccgccgat tccggcggcg ggttcgacac ttggccgctc
     3241 tgcaaactgc ttaaaaccgg gctcaaaaac tgttccatac gctgcctttt ttatcttgac
     3301 ttgttcactc actggctcgc ttatttgcac tgcctctgcc ctgctggact cttgcaaaac
     3361 tgatccgtca gccgttgcag ggtcagctcg taactcatca ctttctgttg tgcctggctg
     3421 accgtccacc agctccacag gctggcgctg gccatcatca acaacgccag gcaggcgctg
     3481 gccaggatcg gccaaagcag catccagcgg agccgctgca gcgtcgcttg gtgcagagag
     3541 gtcagttctt ggccgtggtg ctggatagcg ctcgcggttg tagccagcgc gtcgctggac
     3601 agcgttttca agctcttggc gtgctgctct agctgctgct tcgtcaggtc gtccacggcc
     3661 tgctggtctg cttgcagctt cttctgcaac ttttctgata gttcttgggc tgaaatcact
     3721 ggcatacaaa agtccttgta gttttatcgc cttattggtt ccttcgagct ttattgatac
     3781 ggacttgtca gtggtgcgtg taatttcacc aagttcacct aatgctttaa gcacaccctg
     3841 acggtcggtg acaacgttat tggcgacaag ttttgtcaaa tagtccgtca caactatttt
     3901 tttatccgct ccagacttcc attcgggctt ggcttttccc tgctgaacaa gcctcgcacg
     3961 gctcggatcg tcaggccgcg cccacccttt ttcatgattg aaagcgtccc gcagcgggtc
     4021 aaaggtgtac tcccaacctg gcggcgcgat gttcatggct ttgccggtgg tgagttcgac
     4081 tctaggaata atcgtatgga tgtgaatccc gccattgctt tcgcggtgca caacggcgca
     4141 aaaatcgtat tgatctcttt caagtccgga gaatgcagtg cgttcaaatt catccagcac
     4201 aatgccaatt tcttcatccg ttggcgcatc ttcaggagaa aaattaatta ccccagacgt
     4261 gtagcggtga acagttttaa ggctgtcgat caattttcca gtcagttgcg gattgccgcg
     4321 ccagacttcc acgctggcgc gaatctcgcc cttgtgatct tcttcaccga gcaaataatc
     4381 aatggcatct tgtccaacgc ctgtgccatg gggcaaaaat ttgatgtgca tggtcagctt
     4441 tcactaagct tttctaagct tttctcaatg cctttgagcg tgatcaaaag ctcaaccatg
     4501 gccatcggtt gcgccgtttt gctggcaata ttggccaatt ttgcgatttg atttaggttg
     4561 tttcccatcc cgacaagctg ccgcagcagc ttgggatctg ctcctgaaac cttcgccaag
     4621 cccgcccttt ttcttggtac tgccttgccc aaaggcttgg cctcgtccac tgtcagatgt
     4681 gcgcggagca catccgacag ggtgacgccc tgctctctcg cacgctcagc gaagcgcgct
     4741 accagacttg cgggcaaccg gcaaggcagt agtacgagcg gttctctggc tgggtatttt
     4801 ggcattttgt attgtattgg ctgttaattt ttcatccgct tagcggatga ggacttagca
     4861 gggtctgggg tgcaacccca gcaagccccg tatacaaaaa ccgcttggcg gttttgtata
     4921 cgggtaggct tgctaaaggt atcaaatttt ggcgatattg ttgagttgaa aaacatggta
     4981 acaggtattt ccatccggtg ccagaattcg ggctccaggc ttcgcctctc tccctacggt
     5041 cgattcaccg ggcggtaggg aggaggaggt tttcggggat ccttcaaagg gtcaaggatg
     5101 gctgatctgc gctcccggcc tgggctcctg gtgccgcttt gactgctggc tttttgtagc
     5161 ctgctttgcg catcgccttg gccagcttgt ccatagcatc ccagattttg tcggcttgtt
     5221 tttctgacaa gtccacgaca tcggatgaaa tattatccgc caactggtct gcatagtagg
     5281 gcgtcatggt aatgctgctc ttgcgcgact ctgctagatt tttatccgac cttacggtta
     5341 gccactcagt cagctccttt ttctcatcat cggataggtc tgctatctgc tcagcggata
     5401 aatatgtatc caattcggta gagctatagg aatacgatct tggaaatgaa tgagccagct
     5461 tctggacgca gcgtttcttt tccgggtcat acggtgatcg gagaagctgg acttttttgc
     5521 cttgttcgcg gatatacatt ggatactcct aaaattgttg atatgcctat attctatcat
     5581 gtagttggat aaaatagtta atgttgtcat tgtatttttc tatccaataa ttggatgtta
     5641 tagtatccaa ctattggatt atgctttatc cgactattgg ataaaattgt ttcacgtgaa
     5701 acaataggaa attatccgac tataaccgct agaattttat ccaactatcg gatagatttg
     5761 catccaaatg taaatgatga tattttatcc gacgtctgat aaaatttatc caatagttgg
     5821 ataaattttt atcctatata gtgcgttgtt tttctatcct atagtcggat atatttttat
     5881 ctatagtcgg atagttttgt ttcacgtgaa acaatagaaa actatccaat agttggataa
     5941 aaatctattt gtctataaat gacaaatgtt acaattttgg attgattttt atccaataat
     6001 cggatagtat tttatccaaa aatagctgga taattaaaca ccatttaata tgatgtaaaa
     6061 tcatccaata attggataga attttatctg ttgtttttta gataatattt catccgctag
     6121 ttggatagaa ttctttcaga ctaggtgatt ttcgttaatt tgcttaaaaa ataggcaaca
     6181 accggcctat gcaatgcacc cgcccgccac ctcttcctcc ctaccgcccg gtgaatgacg
     6241 cgaagcggca gaggcgaagc ctggagcccg aattttggtg tttggcaagg ccagtcgagc
     6301 gctaaaagcg cgctggctgt gctgcgcatg tggatgatct tggatagatc atggatgaac
     6361 cccgtttttc gctcttgaag gcgggaacga taaacagggg gttggggaac gataaacagg
     6421 gggttgggga acgataaaca ggggttggcg gataaataa
//
