LOCUS       pGNB2                   8469 bp    DNA     circular     30-NOV-2020
DEFINITION  Uncultured bacterium.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      uncultured bacterium
  ORGANISM  uncultured bacterium
            Unclassified.
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of pGNB2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8469
                     /organism="uncultured bacterium"
                     /mol_type="genomic DNA"
     mobile_element  1..8469
                     /gene="pGNB2"
                     /product="Plasmid: IncQ-3pGNB2"
     CDS             complement(1..882)
                     /gene="repC"
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein C"
                     /translation="MSKHDLTHARHDPAHCLAPGLFRSLKRGERKRGKLDVVHDYGDG
                     KRIEFSGPEPLGADDLRILQGLVAMAGPGGLVLSPEPQTEAGQQLRLFLEPKWEAFQQ
                     DAMVVKGSYRALAREIGYANPDDTKPVRECIERLWKVSIIAQNGRKRQGFRLLSEYAS
                     DEQDGKLYVALNPLIARAVMGGAQHVRIDMDEVRALQTDPARLMHQRLCGWIDPGKSG
                     RVELDTLCGYVWPGQATNPNTLKTRRQAARRALPELAALGWAVSEYAKNKFEIGRPKA
                     THLLGPAPKTQEISHEK"
     CDS             complement(869..1732)
                     /gene="repA"
                     /codon_start=1
                     /transl_table=11
                     /product="Regulatory protein A"
                     /translation="MAINIMEAFQQEPPQLDFIWPGFLAGTVGALVAPGATGKSFWAL
                     EAAMSIACSVAGGDVVNLKPQHTGRVVYLAGEDPEPALIRRVHAIGQHLNQSAREAIA
                     ENLTLEPIMGKRLNVMDEEHLRRVIEYSAGARLIVLDTLSRIHSLDENSNGDMARLVA
                     TLEHVAASTGASVLYLHHVSKGSAREGQTDQQQAARGASALIDNARWCGFVAKMTEDE
                     AKRLSDRAYDRQPIGNDRRGFFVRFGVSKQNYDATPLDQWYERRDGGVLVPVELLEVP
                     NGKKGGSREQA"
     CDS             complement(1803..4244)
                     /gene="mob"
                     /codon_start=1
                     /transl_table=11
                     /product="Relaxase/Mobilisation nuclease domain protein"
                     /translation="MKAKVSRGGGFRGALNYVFDVGKEATHTKNAERVGGNMAGNDPR
                     ELSREFSAVRQLRPDIGKPVWHCSLSLPPGERLSAEKWEAVTTDFMQRMGFDQTNTPW
                     VAVRHQDTDKDHIHIVASRVGLDGKVWLGQWEARSAIEATQELEHTHGLTLTPGLGDA
                     RAERRKLTDKEINMAVRTGDEPPRQRLQRLLDEAVKDKPTAPELADRLHAAGVGVRAN
                     LASTGRMNGFSFEVAGVPFKGSDLGKGYTWAGLQKSGVTYDEARDRAGLERFRPTVAD
                     RGERQDVAAVPEPDARGLEAPTGRSLDRDGADLGTAGPTPTGRDAGSGSLRQGDGHSA
                     QDAGRADAADERERGEGVRAEGRGAGQDHLRPVQQPVRAGNEHQQHEADRGAGGGLAG
                     QSGERTEGHDASRQQTDRGSERDAPAPLAAGAGADSGRGRGRDAGSDWASRFKQASAA
                     KRRAADGGLGQRDLEQGHAQGARVAGTDRQSARELDPTAYLEASGYTVKREGRHLSVK
                     AGGDEAYRVTRQQDGRWLWCDRYGNDGGDNIDLVREIEPGTGYAEAVYRLSGAPTVRQ
                     QPRPSEPKRQPPQLPAQGLAAREHGRDYLKGRGISPDTIEHAEKAGMVRYADGGVLFV
                     GYDRTGTAQNATRRAIAPADPVQKRDLRGSDKIYPPILPGDPAKVWIVEGGPDALALH
                     DIAKRSGQQPPTVIVSGGANVRSFLERDDVQAILKRAERVTVAGENEKNPEAQAKADA
                     GHQKQAQRVAEITGREVRQWTPKPEHGKDLADMNARQVAEIERKRQAEIEAERARNRE
                     LSRKSRGYDGPSFGR"
     CDS             complement(4228..4659)
                     /gene="mobC"
                     /codon_start=1
                     /transl_table=11
                     /product="Bacterial mobilisation protein"
                     /translation="MNTDESKRRRRGPAPLDATDKRGHTVSVRLNDAELARLDSQRDA
                     VQMQRGEYLRAAALHRLPPTIPAANREQWAELARTAANLNQIARHLNEGQRGDGERIG
                     KALGLQLSAELANCLRLLVLVRGDLIGVKHDSEDVEDESEG"
     misc_recomb     4772..4783
                     /gene="oriT"
                     /product="Origin region of transfer"
     CDS             5117..5584
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MQFKRQGRRVQVLAYRGYDKEKRRAIVKMMGSIDVYSYEPSGGL
                     IENLTDEEKTELQSYIETERQAAEKRSRVYYAKSAASRIVEVADTIKAGDFEPSEAWA
                     ADTWAAIEALTKAMRKAGYPKLRKAPQKAADAPMPGQAGLPFGDAPETPESAS"
     repeat_region   5837..5902
                     /gene="iterons"
                     /product="Binding sites for replication initiator protein"
     CDS             6136..6975
                     /gene="aer"
                     /codon_start=1
                     /transl_table=11
                     /product="Aerotaxis receptor"
                     /translation="MRQNLPVTGRNLELPKDTNILSTTSPQSHITYVNPDFIKISGFT
                     EEELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLIKNRCKNGDHYWVSSYVTPI
                     AKNGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSII
                     SSAMAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSI
                     YTGRTDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLRKVRISHMAREHSL
                     VTH"
     CDS             7270..7926
                     /gene="qnrS2"
                     /codon_start=1
                     /transl_table=11
                     /product="Quinolone resistance pentapeptide repeat
                     protein"
                     /translation="METYRHTYRHHSFSHQDLSDITFTACTFIRCDFRRANLRDATFI
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIELRECDLKGANFSRA
                     NFANQVSNRMYFCSAFITGCNLSYANMERVCLEKCELFENRWIGTHLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIASWQQEQLLEALGIVVFPD
                     "
BASE COUNT     1631 a   2497 c   2429 g   1912 t
ORIGIN
        1 tcatttctca tggctaattt cctgtgtttt gggtgctggg ccgagcagat gcgttgcttt
       61 cggcctgccg atttcaaact tgttttttgc gtattcgctg actgcccagc ccaatgcggc
      121 aagctcagga agcgccctgc gagccgcttg gcgtcgcgtc ttgagcgtat tggggttggt
      181 tgcctgcccc ggccagacgt agccgcaaag cgtgtccagt tccacccgcc cggacttgcc
      241 ggggtcgatc cagccgcata ggcgctggtg catcagccgc gccgggtcgg tctgtagcgc
      301 ccgcacctcg tccatgtcga tgcggacatg ctgcgcgcca cccatgacgg cgcgagcaat
      361 caggggattc aaggctacat acagctttcc gtcctgctcg tcgctggcgt actcggacag
      421 caggcggaag ccctgccgct tcctgccgtt ctgggcgatg atggacacct tccaaagccg
      481 ctcgatgcac tcccgaaccg gcttagtgtc gtcggggttg gcgtagccaa tttcgcgggc
      541 aagtgctcga tagctgccct tgaccaccat cgcatcctgc tggaaggctt cccatttcgg
      601 ctcaaggaac agccggagtt gctgcccggc ctcggtctgc ggctcaggtg acagcacaag
      661 gccacccggc ccagccattg ccacaagtcc ttgcaggatg cgcaagtcat cagcgcccag
      721 cggctccggg ccgctgaact cgatgcgctt accgtcgcca tagtcatgca ccacgtccag
      781 cttgccgcgc ttgcgctcgc cacgcttgag gctgcggaat aggccggggg ccagacaatg
      841 cgccgggtcg tgccgggcgt gggtcaggtc atgcttgctc acggctgccc cccttcttgc
      901 cgttcggcac ttccagcagt tccaccggaa ccagcacgcc gccgtctcgg cgctcatacc
      961 actggtcaag cggggtggcg tcgtagttct gtttgctcac gccgaagcgc acgaagaagc
     1021 cgcgccggtc gttgccgatg ggctgacggt cataggcgcg gtcgctcagg cgcttggcct
     1081 cgtcctcggt catcttggcg acgaagccgc accagcgggc gttgtcgatc agtgccgatg
     1141 cgccccgcgc tgcctgctgc tggtcggttt gcccctcgcg ggcgctgccc ttgctgacgt
     1201 ggtgcaggta caggacagac gcgccagtgc tggccgcgac gtgttccagc gtggcgacaa
     1261 gccgcgccat gtcgccgttg ctgttctcgt cgaggctgtg aatccggctc agggtgtcca
     1321 gcacgatcag ccgcgccccg gcgctgtact cgatgacgcg gcgcaagtgc tcctcgtcca
     1381 tgacgttcag gcgcttgccc atgatcggct caagggtcag gttctcggcg atggcttcgc
     1441 gggctgactg gttgaggtgc tggccgatgg cgtgaacgcg ccggatcagg gcgggttcgg
     1501 gatcttcacc ggccagatag accacgcgcc cggtgtgctg cggcttgagg ttcaccacgt
     1561 caccgcctgc cacgctgcaa gcgatggaca tagccgcttc cagcgcccag aagctcttgc
     1621 cggtcgcgcc gggggccacc agtgcgccca cggtgccagc cagaaagccg ggccagatga
     1681 agtccagttg tggcggttcc tgctggaacg cctccataat gttgatagcc atatcggcct
     1741 cctactcagg ttgatgatgg ttagcctgtc ggtcggttca tcacaccgac tgacaggcgc
     1801 tcttatctgc cgaagctggg gccatcatac ccccggctct tgcgtgaaag ctctcgattt
     1861 cgcgctctct cagcctcgat ctcggcctgt cgcttgcgct cgatctctgc cacctgccgg
     1921 gcgttcatgt cggccaagtc tttgccatgc tcgggcttcg gcgtccattg gcgcacctcg
     1981 cgcccggtga tttcggccac ccgctgcgcc tgtttctggt gcccggcgtc ggccttggcc
     2041 tgcgcctcgg ggttcttttc gttttccccg gccacagtga cgcgctcggc ccgcttcagg
     2101 atcgcttgca cgtcgtcacg ctccaagaag ctgcgcacgt tcgcgccgcc tgacacgatg
     2161 acggtgggcg gctgctggcc gctgcgcttg gcgatgtcgt gcagggctag cgcatccggg
     2221 ccaccttcca cgatccagac ctttgccggg tcgcccggca ggatcggcgg atagatcttg
     2281 tcgctgccgc gtaagtcgcg cttctgcacc ggatcagcgg gggcaatggc gcggcgtgtg
     2341 gcgttctgcg cggtgcctgt acggtcgtag ccgacgaaca gcacgccacc gtctgcatag
     2401 cgcaccatgc ccgccttctc ggcgtgctcg atggtgtccg ggctgatgcc ccggcccttg
     2461 aggtagtcgc ggccatgctc gcgggctgcc agcccttgcg ccggtagctg cggcggttgg
     2521 cgtttcggct cgctcgggcg cggttgctgc cggactgtcg gcgcacctga aagccgatag
     2581 acggcctcgg cgtagccggt gccaggttcg atctcgcgca caaggtcgat attgtccccg
     2641 ccgtcgttgc cgtagcggtc gcaccagagc cagcgcccgt cctgctgccg ggtcacgcgg
     2701 tacgcctcat caccgccagc cttgacggac aggtgtcgcc cttcgcgctt cacggtgtag
     2761 ccgctcgcct caaggtaggc ggtcggatct agttcgcggg ccgattgacg atctgttcca
     2821 gcaactcgcg ctccttgggc gtggccttgt tccaaatcgc gttggcccaa tccgccgtct
     2881 gctgcacgtc gcttggcggc actagcctgt ttaaagcgac ttgcccagtc gctaccagca
     2941 tcgcgcccac ggccccggcc agaatcagcc ccagcgcctg ccgccaaagg cgcgggcgcg
     3001 tctcgttcgc tgcctcggtc agtttgttgg cggcttgcgt catgtccttc tgtgcgctca
     3061 ccagattgcc cggcaagtcc gccacctgct ccgcggtctg cttcatgctg ctgatgctcg
     3121 tttccagccc tgacaggctg ttggacaggt cggagatggt cttgtccagc tccccggcct
     3181 tctgctcgaa cgccttcgcc gcgctcgcgc tcatctgctg cgtcagctcg tccagcgtct
     3241 tgcgctgaat ggccgtcacc ttgtcgtagg cttccagaac ctgcgtcacg tcctgttgga
     3301 gtcgggccag cggttccaag gtctgcgccg tctcggtcga gactgcgacc ggtaggcgct
     3361 tcaagtcctc gtgcatcagg ctcagggact gctgcaacgt cctgacgctc tccacgatct
     3421 gcaactgtgg gcctgaatcg ttcaaggccc gcacggtctc tagcttcgtc ataagtcacc
     3481 cctgatttct gtagtcccgc ccatgtgtag cccttgccca agtcgctgcc cttgaacggc
     3541 actccggcca cctcgaagga aaagccgttc atgcgcccgg tgctggcgag gtttgcccgg
     3601 acgcctacgc ctgcggcatg tagccggtcg gccagttctg gcgcggtcgg cttgtccttc
     3661 actgcctcat ccagcagccg ttgcagacgc tggcgcggcg gttcgtcgcc cgttctcacg
     3721 gccatgttga tctccttgtc ggtcagcttc cggcgctcgg cccgcgcatc gcccagcccc
     3781 ggcgtcaggg tcaggccgtg ggtatgctca agctcttggg tcgcctcgat ggcgctgcgg
     3841 gcttcccact ggcccagcca aactttcccg tccagcccta cccggctggc cacgatgtgg
     3901 atgtgatcct tgtccgtgtc ctggtggcgc actgccaccc acggcgtatt ggtctggtca
     3961 aagcccatgc gctgcatgaa gtcagtcgtc acggcttccc acttctcggc gctcaggcgc
     4021 tcaccgggag gcagtgacag cgagcaatgc cagacgggct tgccgatgtc cgggcgcaac
     4081 tggcgcacgg ctgagaactc ccgcgacagt tcgcgggggt cattcccggc catgttgccg
     4141 ccgacacgtt ccgcgttttt cgtgtgcgtg gcttccttgc caacgtcaaa aacgtagttc
     4201 agcgcaccgc gaaaaccgcc accacggcta accttcgctt tcatcttcca cgtcctccga
     4261 gtcgtgcttg acgcctatca agtcgccgcg caccagaacc agcaatcgca ggcagttggc
     4321 aagctcggcg ctcaactgca agcccagcgc cttgccgatg cgctccccgt cgccccgctg
     4381 cccttcgttc aggtgccggg cgatctggtt caggttggcc gctgtgcggg ccaactctgc
     4441 ccactgttcc cggttcgctg ccgggatggt gggcggcagg cgatgcagag cggcggcgcg
     4501 taggtactcg ccccgctgca tctgcaccgc gtcacgctgc gaatccagcc gcgcaagctc
     4561 ggcatcgttg aggcgcaccg aaacggtatg cccgcgcttg tccgtggcgt cgagcggcgc
     4621 aggcccacgg cggcggcgct tggattcgtc agtgttcatg ggcgaagccc tccagtaagt
     4681 gaagcgaagc gaaacgcgca gcaacccccc gaccgaaggg agcgggggcg taggccgaag
     4741 gccggagggt gagaaccaag cgaagcgtag gttacacctt gctccatgcc aaaccaccgc
     4801 gcaaatacca taatagcaca tctccatgca agcaccgaac agccacccac ccggcccgct
     4861 cgctgcgctc tcggcccgac cgcccaaggg cggtaatggg ccagcgggga gagggggtgg
     4921 gcaaggcgcc ggggcgaact gatacaatgc aactatggta tcgcaagaac ggcagactgt
     4981 cgagcgttac tcaatccgac agtgcaaacc tatttgagtg cgacggtcgg attgaaaaat
     5041 aggccgacaa taaacactcg acagcgtgcg actgtcgaac tataatataa tcacacagtc
     5101 aaacggagaa accgccatgc agtttaagcg ccaaggtcgc cgggttcagg tgctcgccta
     5161 ccgggggtat gacaaggaaa aacgccgcgc catcgtcaag atgatggggt ctattgacgt
     5221 atattcttac gagccgtcag gcggcctaat tgagaatctg acagacgaag aaaaaacaga
     5281 actacagtca tacatcgaaa cagaacgaca ggcagcagaa aagagaagcc gagtgtatta
     5341 cgctaagtct gccgccagtc gtattgttga ggttgccgac acgatcaagg ccggggactt
     5401 tgagccgtcc gaggcatggg cggcggatac ctgggcggca atcgaggcgc tgaccaaggc
     5461 catgcgcaag gcgggctacc cgaagctccg caaagcgccc cagaaagccg cagacgcgcc
     5521 gatgccgggt caggcggggt tgccctttgg ggatgcgcca gaaacgccag aaagcgcctc
     5581 ctgaggggtt taggccggga aaccggcacc cccccccctc tccccgctgg ccctttaccc
     5641 ccggaggggg tcgccgaagg cggggggaga ggtgttcgcc ttgcgcatgg tcttggccgg
     5701 gcatttgagg caatgccaga ccgcgccgaa ggggcgcggg ctgtgctgcg cctagtggag
     5761 attcttagag aatcttggat ggattttgcg aatcgccagc aaatccgcgc cccgtctggg
     5821 ttttccagat cggggcgggt ggagttaccg tgggggttgg gtggagttac cgtgggggtt
     5881 gggtggagtt accgtggggg ttgggtggag ttaaacaggg ggtcacacat actcgcacca
     5941 gatcacgctg ccccagtgct cgccggtttc cggctcaatg gctacgttat cgaagcgccc
     6001 tcctgagtag atcaggcaac gcttcccaat cagcctagcg gggtcggtgt agccatgcta
     6061 gcttatcgta acgctaaaga aattgggctt aatgccgagt acaataaaat aaaacagcca
     6121 accattgagg ctcttatgcg ccagaattta ccagtgacgg gtcgaaactt agaactccca
     6181 aaagatacca atattctttc gactacctcc cctcaaagcc atatcacgta cgttaatcct
     6241 gacttcatta aaatcagtgg tttcactgag gaagaactat taggccagcc tcacaacatc
     6301 gtaagacacc cagatatgcc gcctgctgca tttgagcata tgtggagtac attaaaatct
     6361 ggccgctcat ggatggggct aataaaaaat cgctgtaaaa atggcgacca ctattgggta
     6421 agttcttatg taacgccaat agctaagaat ggttcgattg ttgaatacca gtctgtaagg
     6481 accaagcctg aacctgagca ggttttggct gcggaaaaat tatatgctca attgagaagc
     6541 gggaaggccg cgaggccgaa attggctgct agcttttccg tgaaaatact cttgctcata
     6601 tggggtagta ttatatcgag cgcaatggct gccggcatgc ttactgatac atcaataagc
     6661 agcttattgt tagccacttt aatgtcagga agcttaagct ctgttagtgt tttggctatt
     6721 ctctctcctc ttggaagact ggttgaaaga gccaggaata tttccaataa cccattaagt
     6781 caatccatct acactgggcg caccgatgag tttggccaaa tagagtttgc tttacgaatg
     6841 atgcaagctg aaacaggcgc catagtaggt cgcataggtg atgcatcaaa tcggcttagc
     6901 gaacacaccc gaggcctact aaggaaggtg cgaataagtc atatggcacg agagcattca
     6961 ctggtaaccc attgatatta aatgactaaa attttcattt tttgttcttg ggatacttgt
     7021 tcgcactttc cctaatggcg gacatcagta atctattggg ttccttttct ctgtggcttt
     7081 ttaagcggat aacttttttc aatggtgccg cgtactttga acgttaacat agaggctcag
     7141 ggcgctattt taccccctta aaataggcaa aatgagggtt tttaagatct tgatttttag
     7201 tgtttttatt ttggtgatgt gatgtggctt tgatggtagt gttgcgtcca gttcatcata
     7261 ggagtcaaca tggaaaccta ccgtcacaca tatcgacacc acagtttttc acatcaagat
     7321 ctaagtgata ttactttcac tgcttgcacc tttatccgat gcgattttcg acgtgctaac
     7381 ttgcgtgatg cgacatttat taactgcaag ttcattgaac agggtgatat cgaaggttgc
     7441 cattttgatg tcgcagacct tcgcgatgca agtttccaac aatgccagct tgcgatggca
     7501 aactttagta acgccaattg ctacggtatt gagttacgtg agtgtgattt aaaaggggcc
     7561 aacttttccc gagcaaactt tgccaatcaa gtgagtaatc gtatgtactt ttgctcagcc
     7621 tttattactg gatgtaacct gtcttatgcc aatatggagc gggtctgttt agaaaaatgt
     7681 gagctgtttg aaaatcgctg gatagggact cacctcgcgg gcgcatcact gaaagagtca
     7741 gacttaagtc gaggtgtttt ttctgaagat gtctggggac agtttagcct acagggtgct
     7801 aatttatgtc acgccgaact cgacggttta gatcctcgaa aagtcgatac atcaggtatc
     7861 aaaattgcca gctggcaaca agaacagctt ctcgaagcgt tgggtattgt tgtttttcct
     7921 gactagatgc ttacgaatac ctgctgataa actatacaca acattaatac attttctaat
     7981 tcaaatcacc agagctagat caatacttct agctcattct tgtccagaaa cgtgtttgaa
     8041 tggggccaat caatctgtgg atgctcaaga caaccagtat gatactttat tactcagttg
     8101 tagcgtgact cagattttgt agtaggaatt agtatgaatc tttatttctg ggtgagtatc
     8161 atttagaacg gtacaaacag ggttacttga ctgcttagta tgaaacttta ttcctctcct
     8221 acaacacctc agagcacgta ctctgatgtc agatgaaccg tcctgttcca acgtcttgga
     8281 tccacaactg ccgcgctgcg cttggctgct ggcttgttca gtgagtcgaa aagatgcctc
     8341 ccctcaaagc ccagcttctg ctcgaagaat ccctgtccgc atactttccg gaaatggtgt
     8401 tcacagattc cggaatcact gttcaacttc accggaatac gcacttcgac tgtcggcggc
     8461 gtttcccaa
//
