LOCUS       pTF-FC2                10948 bp    DNA     circular     30-NOV-2020
DEFINITION  Thiobacillus ferrooxidans.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Thiobacillus ferrooxidans
  ORGANISM  Thiobacillus ferrooxidans
            Unclassified.
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of pTF-FC2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10948)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10948
                     /organism="Thiobacillus ferrooxidans"
                     /mol_type="genomic DNA"
     mobile_element  1..10948
                     /gene="pTF-FC2"
                     /product="Plasmid: IncQ-2pTF-FC2"
     CDS             1..873
                     /gene="repA"
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein A"
                     /translation="MALDIMAAFTNEPPELDFIWPGFLAGTVGALVAPGATGKSFFAL
                     EAAMSIACSVAGGDLVGLTPAHTGRVVYLAGEDPQPALVRRVHAIGQHLNQSAREAIA
                     ENLMLEPIMGKRLNVMDDAHLRRVIDYSAGARLIVLDTLSRIHILDENSNGDMAHLVS
                     VLEHIAATTGAAVLYLHHVNKGSARDGQTDQQQAARGASALIDNARWCGYVAKMTEQE
                     AERMSDRGFDRSPSGNERRGLFVRFGVSKQNYDATPLDRWYQRHSGGVLLPVELQEAI
                     SNGAGKKGGKRNEL"
     CDS             833..1732
                     /gene="repC"
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein C"
                     /translation="MEQEKKGESAMSYDLTHARHDPAHCLTPGLFRSLKRGERKRLKL
                     DVTYNYGDDSIRFWGPEPLGGDDLRVLQGLVAMAAISGDNGRGIVLRHETESEAGQQL
                     RLWLDMRWDAIEKDTMVAKGSFRQLARELGYAEDGGSQFKTIRESIERLWAVSVIVER
                     GGKRQGFRILSEYASDEQEGKLFVALNPRLADAVMGERPHTRINMAEVRKLETDPARL
                     LHQRLCGWIDPGKSGKAEIDTLCGYVWPDAANDEAMKKRRQTARKALVELAAVGWTVN
                     EYAKGKWEISRPNPRRNVPQPPA"
     repeat_region   1697..1762
                     /gene="iterons"
                     /product="RepC-binding sites; regulation of replication"
     repeat_region   2265..2269
                     /gene="DR_Tn5467"
                     /product="Tn5467 direct repeat; target site duplication
                     signals for transposition"
     mobile_element  2270..5570
                     /gene="Tn5467"
                     /product="Unit transposon: Tn5467"
     repeat_region   2270..2307
                     /gene="IRL_Tn5467"
                     /product="Tn5467 inverted repeat left"
     CDS             complement(2393..2689)
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn5467 transposase"
                     /translation="MFILDWLQSVELRRRVNAGLNKGEARNALARAVFFNRLGESRDR
                     SFEQQRYRRSGLNLVTTAIVLWNTVYLERAAHALRGSGHGVDDRLLQYLSPLGW"
     CDS             complement(2641..3009)
                     /gene="tnpR"
                     /codon_start=1
                     /transl_table=11
                     /product="Resolvase"
                     /translation="MSEGGTVAVHSMDRLARNLDDLRRLVQKLTQRGVRIEFVKECLK
                     FTGEDSPMANLMLSVMKAFAEFERALIRERHVKALRWPSSRARIRARTAWPWHCATWG
                     ASSAPCSFWTGCKAWSCAAV"
     misc_recomb     complement(3180..3397)
                     /gene="res"
                     /product="Resolution site"
     CDS             complement(3326..4546)
                     /gene="orf1221"
                     /codon_start=1
                     /transl_table=11
                     /product="Unknown but similar to membrane translocases"
                     /translation="MVLYRMPLSIKSESACFECSQPRPLSSFSRRTWSRRSYLHSSAF
                     GTSVQTAGLVVPAYLIPYGIATLIFGLLADRLGVQRVMFASLAAFAVLTALTASATTI
                     AQITLWRVVTGLGASGVVPLALVLVGKLFPYEQRGRPLGWLFGAMAGGMAFGSPLGVM
                     LVPFIGWRGLFLMVGVAGGVVLLLFLPYRRMIAAAMQPVGGTFGELFRGYANLLGTPR
                     GQRTYAYVLVNSMFHSGVFTWLGVYLERRYGLGPVGIGLALLGYGIPGLLFGPLIGRT
                     ADKWGRARLLPIGLGLSTLGAAALLWDFPLILAPIVAMVLSLGYDMTQPLFAGIVTSL
                     GGKRPGQAMGLNVFALFVGFGLGSLIFGEMLRFGFGAALGTFAAVELMAALFALRLFR
                     SERRQRKRIVPARP"
     CDS             complement(4722..5135)
                     /gene="merR"
                     /codon_start=1
                     /transl_table=11
                     /product="Mercuric resistance transcriptional regulator
                     MerR"
                     /translation="MHTIGKVATLAGVSTDTLRYYEKEHLIEPASRTAAGYRLYNDEA
                     VRRIRFIKTAQHCGFSLSDIQELLTLKRTDSACCEDVRSVAIEKRLRIARKLRVLQAM
                     SAALNDLIQSCEGGAAATDDCPILAALENSLHRVS"
     CDS             complement(5256..5513)
                     /gene="orf258"
                     /codon_start=1
                     /transl_table=11
                     /product="Thioredoxin-like protein"
                     /translation="MTTKRKVEVFSAGCPSCQTAIELVNRLACGSCEVSILDMNDINV
                     AKRARDLGVRSVPAVAINGQLASCCSGSGIEEQALRWPRQA"
     repeat_region   5733..5770
                     /gene="IRR_Tn5467"
                     /product="Tn5467 inverted repeat right"
     repeat_region   5771..5775
                     /gene="DR_Tn5467"
                     /product="Tn5467 direct repeat; target site duplication
                     signals for transposition"
     CDS             complement(5908..6420)
                     /gene="orf513"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MGVAMIDALNIATMPIADKVHRHMLASYYALQLDALQAEAKRLG
                     YFFAQADTAATMPPVLADCLAWAVEYRRRCYLYPNCPESWERHTADSMSDGYAQEHCR
                     SMLAALAALGIRLQEGQQAYALLAAEECRQREKASLPPEPSPLSEVEVSSFVDEFFTK
                     LDAPKEEVPT"
     CDS             complement(6405..7046)
                     /gene="mobE"
                     /codon_start=1
                     /transl_table=11
                     /product="Mobilization protein MobE"
                     /translation="MSDLDDSFAKLLGRQPSDAERQSLYRVRDALGLKNNDALWLVLM
                     ALQHYQGQYEKFPQAIAQAAKDTLVNFKVTADATVKASAEAAKADLAQAVAAAAQEVA
                     HNTSAKQMWQWAAGCIAVAFLCVGLFGWYMHSSGKDSGYQAGYGAGYTEAKDEKAAAA
                     WANTPEGRTAYRFAQSGELQRLARCSGKGWKVEKGACYPYPVANEGTYGWALP"
     CDS             complement(7039..7722)
                     /gene="mobD"
                     /codon_start=1
                     /transl_table=11
                     /product="Mobilization protein MobD"
                     /translation="MAKSIYLIGGSKGGVGKSLVTMATVDYLQERGESVLLIESDTSN
                     PDVWKAYKESTETELINLDEADGWIQLVNLCDSKPDSVVVINAAARNNKGVSAYGETL
                     NSTLAELKRKLVTLWVINRQRDSLELLKEYMDAIPNADVHVVRNGHFGEEKKFELYNG
                     SKLRTAVEERGGQSVTFPDMADRVSDDIYSKRMSISVALKELPIGNRAELTRWRNEAK
                     KVLEGVIHE"
     CDS             complement(7735..8091)
                     /gene="mobC"
                     /codon_start=1
                     /transl_table=11
                     /product="Mobilization protein MobC"
                     /translation="MKYTTEQLEAIASKLRDMPQVEKKKQEHSKQEAVRVLSKEIAAL
                     QKRGYTLDQISETLRGEGLSIATPTLKSYLQRAKPTKKAPVQAPGDTPPPAPAVKKPA
                     DTSKATFTPKPDSDDI"
     misc_recomb     8209..8258
                     /gene="oriT"
                     /product="Origin region of transfer"
     CDS             8399..8719
                     /gene="mobB"
                     /codon_start=1
                     /transl_table=11
                     /product="Mobilization protein MobB"
                     /translation="MPFETQGPEPLDAVINVRLTAAEKARLKEDADLAGLSMSELVRR
                     RYFGRPIIASADAVMLKELRRIGGLLKHIHNESGGVYSKDTAGALVVLKAYIGKLSRD
                     RQEG"
     CDS             8871..10166
                     /gene="mobA/repB"
                     /codon_start=1
                     /transl_table=11
                     /product="Mobilization protein MobA/Replication protein B"
                     /translation="MIALSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDEL
                     GVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINNGFDIEAAHKAIARIENAQG
                     WQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAIEDGAPII
                     KKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQ
                     PPQRQQVAQREPEPIKPDVPGWKDYITGRKAHYSEKNADKLVQDKRQEQERKQLAEQQ
                     KARRDELMRGNWKGKGEVLNAMRSVIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDL
                     EQWQRMQKSPELAEQWRHRASEPQRIEGDRSEPPTPRDIRAYQPEIVGQQVHYSRKEE
                     GGRGGGVSFVDKGKSIDDRARQLAGNESLALRGGCDILV"
     CDS             10191..10415
                     /gene="pasA"
                     /codon_start=1
                     /transl_table=11
                     /product="Antitoxin protein PasA"
                     /translation="MLAIRLPAEVETRLEALAQATGRTKTFYAREAILEHLDDLEDLY
                     LAEQRLIDIRAGKTQTVPLEEVMKRYGMEG"
     CDS             10399..10671
                     /gene="pasB"
                     /codon_start=1
                     /transl_table=11
                     /product="Toxin protein PasB"
                     /translation="MAWKVELDPAAERELGKIDQQTARRILAFLHGRVAQLDDPRSIG
                     EALKGSKLGAFWKYRVGDWRIIASIEDGALRILVMRIGNRKEVYRQ"
     CDS             10668..10883
                     /gene="pasC"
                     /codon_start=1
                     /transl_table=11
                     /product="Enhancer of toxin-antidote neutralization"
                     /translation="MIEYSYQIDPRPSDLGGGWRLRLLESGEEVGGGVFPLSEYATAE
                     NAEEAATYAYEDALAEASAWLASRGEN"
BASE COUNT     2327 a   3238 c   3258 g   2125 t
ORIGIN
        1 atggctttag acattatggc ggccttcacc aatgagccgc cagaacttga tttcatctgg
       61 cccggattct tggccggaac cgtgggcgca cttgtcgcac ctggcgcaac tggcaagagc
      121 ttttttgctc ttgaagcggc catgtcaatc gcttgcagtg tggcaggcgg cgaccttgtg
      181 ggactaaccc cggcgcacac cgggcgcgtg gtttatctcg ctggcgaaga tccacagccc
      241 gcccttgtgc gacgtgtcca cgccatcggc cagcacctca accagtcggc ccgcgaagcc
      301 atcgctgaga acctgatgct tgagccgatc atgggcaagc ggctaaacgt gatggacgac
      361 gcgcacttgc gccgcgtcat cgactacagc gcaggggccc ggctgattgt gctggacacc
      421 ctgagccgga ttcacatcct cgacgagaac agcaatggcg acatggccca ccttgtttcc
      481 gtgttggaac acatcgcggc gaccaccggg gcggctgtcc tgtacctgca ccacgtcaac
      541 aagggcagcg cccgcgacgg ccagaccgac cagcagcagg cagcgcgggg cgcgtctgcc
      601 ctgatcgaca acgccagatg gtgcggctat gtcgccaaaa tgacggagca ggaagccgag
      661 cgcatgagtg accggggctt tgatcgttcg ccatccggca acgagcggcg cggccttttt
      721 gtccgctttg gcgtgagcaa gcagaactac gacgcgaccc cgctagaccg ctggtatcag
      781 cggcacagcg gcggggtgtt gttgcccgtt gaactacagg aggcaatcag caatggagca
      841 ggaaaaaaag ggggaaagcg caatgagcta tgacctcacc catgcgcggc acgaccccgc
      901 gcattgcctc acgccggggc ttttccgcag tctcaagcgc ggagaacgaa agaggctcaa
      961 gctcgatgtg acctacaact acggagatga ctcaatccgt ttttgggggc ctgaaccact
     1021 tggcggcgat gacttgcgcg tattgcaagg gctggtggca atggctgcaa tttccggaga
     1081 taacgggcgc ggcatcgtgc tacggcacga aacggaatca gaagcaggcc agcaactccg
     1141 cctatggctt gatatgcggt gggacgccat agagaaagat acgatggtag ccaagggcag
     1201 cttccgccag ttggcccgag aacttggcta cgccgaagat ggaggaagtc agtttaaaac
     1261 catccgggaa agcatcgaac ggctttgggc ggtatcggtg attgtcgaaa gaggtggtaa
     1321 gcggcaaggg ttccgcattc tgtccgagta cgcgagcgac gagcaagaag gcaagttatt
     1381 tgttgcgctt aatccccggc tggcggatgc ggttatggga gagcgcccgc acacccgcat
     1441 caacatggca gaagttcgca agctggaaac agacccggca cggctgctac accagcggct
     1501 atgtggctgg attgaccccg gaaagtctgg caaagctgaa atcgacacgc tgtgcggtta
     1561 tgtatggcca gacgcagcca acgatgaagc aatgaaaaag cgccgccaga ccgcgcgcaa
     1621 ggcgcttgtc gagcttgccg ccgttggttg gacggtgaac gagtacgcca agggcaagtg
     1681 ggaaatcagc aggcccaacc cccggcgtaa cgttccccaa cccccggcgt aacgttcccc
     1741 aacccccggc gtaacgttcc ccgccgaaat ctgaaaaacc tagcaacggc gcgggtttgc
     1801 gggcgattcg caaaatccct ccatgatcta tccaagatca tccactaggc gcggtacttt
     1861 tcgccgccct tgagggcgtc aaaagtcctt accaaaaacc ccaaaagaaa cggccgggac
     1921 aagcccggcc agacacaccc cgccccgccc cgctcgcctt cattcttcca ccgggacaat
     1981 ggacaccatc acccggtagc gtttggcctc tccggcaggt agcgcagcgg ccagcttggc
     2041 gagcgtttcg gctggcttgt cggtcgtgct tgtggagcac atcgcctcat acccgaacag
     2101 aagccatcag aatcgcctac agcggatttt tggatgttct ggctgccttg agctagggtt
     2161 ggtaaagaaa acgcctatgg ctgtttgcgg ggcttctgcg agcattgccg ggacggtctt
     2221 gggcttgctt gtgccgttga ggcgaaaaac gccaccgcca ggacaagcag gggtcgtctc
     2281 agaaaacgga aaataaagca cgctaagccg gttgcagccg tagcggcctg aactcgcccg
     2341 cgccgatcgt ggcgctgctg cgccacaggt aatcgccggt caggttgatg tgttaccaac
     2401 ccagcggcga caggtactgc aacagccggt catcgacgcc gtgaccgctg ccgcgcagcg
     2461 catgtgctgc acgctccaga tagaccgtgt tccataacac gatggccgtc gtcaccaggt
     2521 tgaggccgct gcggcgatag cgctgctgct cgaaactgcg gtcgcggctt tcacctaggc
     2581 ggttgaagaa cacggcacgg gccaaggcgt tgcgggcttc gcctttgttc agcccagcgt
     2641 tcacacggcg gcgcaactcc acgctttgca gccagtccag aatgaacatg gtgcgctcga
     2701 tgcgccccac gtcgcgcaat gccacggcca ggccgttctg gcgcggatac gcgcccggct
     2761 gcttggccag cgcaatgcct tcacgtgccg ctcccgaatc aaggcgcgct cgaattcggc
     2821 aaatgccttc atcaccgaca gcatgaggtt cgccatcggc gagtcctcgc cggtgaactt
     2881 caggcattcc ttgacgaact cgatgcgcac cccgcgctgg gtgagctttt gcaccaagcg
     2941 gcgcaagtca tcgagattgc gtgccaagcg atccatgctg tgcaccgcca cggtaccgcc
     3001 ctcactcacg aagacaagca gcgcatcgcg agttccggcc gttgggtgtc cttgccgatg
     3061 ccttgtcggt gaacaccttg ttcacttgga cttgttcaag ctgccgctct gggttctggt
     3121 cgaagctgct gacccggaca tagccgatac gttgaccttt taagacgcct ccgatgctga
     3181 atgtgtcagg aaagaatcta tgatgtttgg gggaatgtgt caatgaattg caaatgcaat
     3241 cctatcttga cgtctctttg tgtatcagcc tgacatcaag atagggtata gctcaaaatg
     3301 acggttgttc gtttagttcg tccccttagg gcctggcggg gactatcctt ttgcgctggc
     3361 gacgctctga acggaacaat cggagggcga acagcgcagc catcagctcg accgcagcga
     3421 atgtccccag cgctgccccg aagccgaagc gaagcatctc gccgaagatg aggctgccca
     3481 aaccaaagcc aacgaacaga gcgaatacgt tcaaacccat ggcttgccct ggtcgcttgc
     3541 ctccgagtga agtaacaatg cccgcaaata gcggctgcgt catgtcgtag ccaagggaca
     3601 acaccatcgc tacgatgggt gcgagaatca aagggaagtc ccacagtaaa gctgcggcac
     3661 cgagagtgct aagtcctagc ccgatgggca gcaacctggc tctcccccac ttatccgccg
     3721 tgcgaccgat caatggtccg aagagaagtc cggggatgcc atatccaagt aacgcgaggc
     3781 cgatgccgac cggcccaaga ccgtagcgcc gttcgagata cacgccgagc caggtgaaga
     3841 cgccggaatg aaacatcgag ttgaccagca cataggcgta ggtgcgctgt cccctcgggg
     3901 tgccgagcag gttcgcgtag ccgcgaaaca gctcgccgaa cgtaccgccc actggctgca
     3961 tcgcagcggc aatcatgcga cgatagggca ggaacaatag taggacaacg cctccagcca
     4021 ctcccaccat caggaacagt cctcgccagc cgatgaacgg caccagcatc acgcccaatg
     4081 gcgagccgaa ggccatcccg ccagccatgg cgccaaacag ccagcccagc gggcggcctc
     4141 gttgttcata ggggaacagc ttgccgacga ggacgagtgc gagtggtacg acaccactgg
     4201 cgccaaggcc cgtgacaact cgccacagcg tgatttgtgc gatggtcgtc gcgctcgccg
     4261 tcaacgctgt gagcactgcg aacgctgcca gcgaggcgaa catgacgcgt tggacgccaa
     4321 gccgatccgc caggaggcca aagattaagg tcgcgatgcc atacggaatc aagtaggccg
     4381 gaaccaccag accggccgtt tgcaccgagg tgccgaaggc gctcgagtga aggtatgatc
     4441 ggcgcgacca tgtacgcctg gaaaaagatg ataaaggtcg cggctgagag cattcgaagc
     4501 aggcgctctc gctcttgatg ctcaagggca tgcggtacag caccatgcat catgttgatt
     4561 ctccgtgaag tgggatacgg caccggctct agatcgccag cgccggaatg acaaagaaat
     4621 acgcgttcag catgtagaag tcgcgatggt ccgcagctca tcctgtgctg ctgcgcaccg
     4681 gttgcaccct ggcgcggcaa tgacttcaat tttgaccgtc atcacgacac cctgtgcaag
     4741 ctgttttcca gcgcagccag aatggggcag tcgtcggtcg ctgcagcacc gccttcgcaa
     4801 ctctggatca agtcattgag ggcggccgac atggcctgca agacccgtag tttgcgcgcg
     4861 atgcgcagcc tcttctcgat ggccacgctg cgcacatcct cgcaacaggc actatcagtt
     4921 cgcttcagcg tcagcaactc ctgaatgtcc gacaacgaga atccgcagtg ctgagcggtt
     4981 ttgataaaac ggatgcgccg caccgcttca tcgttgtaca gccggtagcc cgcagcggtt
     5041 ctagatgcag gttcgatcag gtgctctttt tcatagtagc gcagcgtatc ggtgctgact
     5101 ccggctagcg ttgccacctt gccgatggtg tgcatggtgt cctccatttg taagtgttaa
     5161 tataaacttt ggagtgaaca ccatggtcaa gagttccgcc taagaaatta atcgcgagct
     5221 tccagcgaga ggcacgcgcg tgccgatggg gaggcctagg cttgccgagg ccagcgaagc
     5281 gcctgttcct cgatgccgct gccggagcag cacgaagcca gctggccgtt gatggcgacc
     5341 gccggcaccg accgaacacc gagatcacgg gcgcgtttgg ccacattgat gtcgttcata
     5401 tcgaggattg agacctcgca cgaaccgcat gcgaggcgat tgacgagttc aatggcggtc
     5461 tggcaggagg ggcatcccgc gctgaaaact tcgactttgc gtttggtagt catggtattt
     5521 ctccaaaaaa gtgtggtcag gtgcgcgaga actcccggcc ggagaagcca tgagcgccgc
     5581 gcgtcattac gatagacctt ggcccttact tcaacgtcaa gtactaactg agcgggttat
     5641 gcggtttcgc gcaacgcccg gatcgcccgc accgggaaca ggaaaacagc cccttcagtc
     5701 cgtgaagcag catatgcgca cgcttgtcgt gccttagcgt gctttaattt ccgttttctg
     5761 agacgacccc aagcagcaag ccaccggcca cgatgaacgg ggcgcgactt gtccgccgtt
     5821 tcggctggat ggctggcagg tctggcgctt cctcgtactg gccctcatcg aacaggccgg
     5881 tttcaaagtc gatcttggcg gttgtgctca tgtcgggact tcctccttgg gtgcgtccag
     5941 cttggtgaaa aactcatcga cgaagctgga cacttcgact tccgagagtg gcgaaggctc
     6001 gggtggcaag ctggctttct cgcgttggcg gcattcctcc gccgccagca gggcgtaggc
     6061 ttgctggcct tcctgcaaac ggatgccgag cgcggccagc gccgcgagca tcgagcggca
     6121 atgctcttgg gcgtaaccgt cgctcatgct gtcggcggtg tggcgttccc agctttccgg
     6181 gcaattcggg tacaggtagc acctgcgccg gtactcgacc gcccacgcca agcaatcggc
     6241 cagcactggc ggcatggttg ccgccgtgtc ggcttgcgcg aagaagtagc cgagccgctt
     6301 ggcttcggcc tgcaaggcat ccagttgcaa ggcgtagtag cttgccagca tgtgccgatg
     6361 caccttgtct gcaatcggca tcgtggcgat gttgagcgcg tcgatcatgg caacgcccat
     6421 ccgtaagtgc cctcattcgc tactgggtag gggtagcaag cgcctttctc gaccttccag
     6481 cccttgccac tgcaccgggc aaggcgttgc agttcgccgg actgggcgaa gcggtaggcc
     6541 gttcgacctt ctggcgtgtt tgcccatgcc gccgccgctt tctcgtcctt ggcttccgtg
     6601 tagcccgcgc cgtatccggc ttggtagccg gaatccttgc cgctgctgtg catgtaccag
     6661 ccgaacaggc cgacgcacag gaaggccacg gcgatgcagc ccgccgccca ctgccacatc
     6721 tgctttgcag atgtgttgtg cgcgacctct tgcgctgctg ctgctaccgc ctgcgctaaa
     6781 tccgccttgg ctgcttccgc tgatgctttc acggttgcat ccgccgttac cttgaaattg
     6841 accaaggtat ctttggctgc ttgtgctatc gcttgcggga acttctcata ctggccttgg
     6901 tagtgctgca aggccatgag caccagccag agcgcatcat tgtttttcag cccgagtgca
     6961 tcccgcacac ggtaaaggct ttgccgctcc gcgtcactgg gttgcctgcc gagcagcttt
     7021 gcaaagctgt cgtcaaggtc actcatggat tacgccctcc aatacctttt tagcctcatt
     7081 gcgccaccgg gtaagttccg cacggttgcc aattggcagt tccttgagtg ctaccgaaat
     7141 gctcatgcgc ttgctgtaaa tgtcatcgct cacccggtcg gccatatccg ggaatgtcac
     7201 cgactgcccg ccgcgttcct ctactgccgt gcgcagcttt gagccgttgt aaagctcgaa
     7261 tttcttttcc tctccgaagt gtccattgcg caccacatga acatccgcgt tcgggattgc
     7321 atccatgtat tccttgagca gttcgaggct atcccgttgg cggttaatca cccacagcgt
     7381 gaccagcttg cgcttgagtt ccgcaagtgt gctattcaat gtttcaccgt aggcgctcac
     7441 tcccttgtta ttcctcgcag ccgcgttaat taccacgacg ctatccggct tgctatcgca
     7501 caagttcact agctgaatcc atccgtccgc ctcatccaga ttgataagct ccgtttccgt
     7561 gctttccttg tatgccttcc acacgtccgg gttagaggtg tcggattcaa tcagcaatac
     7621 gctttcgccg cgttcttgca ggtaatcaac cgttgccatc gtgacaagcg acttgccaac
     7681 gccgcccttg ctgccgccga tcaggtaaat gctttttgcc atgttgctta ctccttaaat
     7741 gtcgtcgctg tcgggttttg gggtaaatgt cgccttcgat gtatccgcag gcttcttgac
     7801 ggcgggggcg ggcgggggtg tgtctcccgg cgcttgcacc ggggcttttt tcgttggctt
     7861 tgctctctgc aaatagctct tgagtgtcgg ggttgcgatg ctcagacctt caccgcgaag
     7921 cgtttccgaa atctggtcaa gcgtgtatcc gcgcttttgc aaagccgcga tctcttttga
     7981 tagcacgcgc acggcctctt gcttgctgtg ctcctgcttc tttttttcga cttgtggcat
     8041 atcacgcagc ttcgatgcga tagcctctag ctgctctgtc gtgtatttca ttctgctaac
     8101 tcctttgctt actggtttcg gagttgacag catagcgcct tttatgcttg ttgcaaagtg
     8161 catctagtgt gcctaaactg ctgatatgat agtagagcgg ttgagcaata caggatgacc
     8221 gttgtaacac acatggcttc gccacgtggt tccaacggac gcctgcccat tcggcgcttc
     8281 gcttctcacg gtctggctta tcctgcaagg ggtatcccct tgaccccgga cagtgcggcg
     8341 ctctcgcttc ttactgtcca agacacaccc cgcccgcccc ccgcgagaag gagacacgat
     8401 gccatttgaa acgcaggggc cggaaccgct ggacgccgtt atcaatgtac ggctgaccgc
     8461 agcggagaag gcccgcttga aagaggatgc cgaccttgcc gggttgagca tgtcggagct
     8521 tgtccgccgc cgctactttg gcaggccgat cattgccagc gccgacgcgg taatgctcaa
     8581 agagttgcgc cgcatcggtg gattgcttaa gcacatccac aacgagagcg gaggcgtgta
     8641 cagcaaggac acagccgggg cgcttgtggt cttgaaagcc tatatcggga aactgagccg
     8701 tgatcgtcaa gaaggttaag aacccgcaga aggcagcaag caaggcggtt cgcgtttccc
     8761 gcctaactgg ctacatccgt gagcctgagc gtgagaactc gcaggagaag tgcatccatg
     8821 ccggggcgcg tggattcatc accgatgaac cacagagcca gacggcagaa atgattgcct
     8881 tgtcacaaga ggcagtgcga agcaaggata cgatcaatca ctatgtcctg agttggcgag
     8941 aaggcgagca gcccagcccg gagcaggtcg aagaagcggt aagcattttc atggatgagc
     9001 taggtgttaa agaccatcaa gccatctatg gtttgcacgc cgacacggac aacttgcacc
     9061 tgcaccttgc catcaaccgc gtgcatcctg aaacgctcaa ggtggtgaag atcaataacg
     9121 gcttcgacat tgaggcagcg cacaaggcga tagcacgaat cgagaacgca caaggctggc
     9181 agcgtgagca gaacgggcgt tatcaggtgt tggagaatgg tgagttaggc cgggagcaca
     9241 tcgacaagga caagccgcgc cagccagccc agccgaagcg cgacatggaa aaccgcaccg
     9301 gggagaaatc cgccgaacgc atcgccattg aggacggcgc accgatcatc aaaaaggcgc
     9361 agacgtggga gcagttgcac cgcgagttag cagccaaggg gatgcgctac gagaaaacag
     9421 gcagcggggc gacgctcttt gtcggtgatg ttggcgtgaa ggccagcagc gcagaccggg
     9481 acgcgagcct atccaagttg caaaagcgac tcggagctta ccagccgccg cagcgtcagc
     9541 aggtagcaca gcgcgagccg gagccgatca agccagacgt gccgggatgg aaggactaca
     9601 tcaccgggcg caaggcgcac tactcggaga agaacgcgga caagctggta caggacaagc
     9661 ggcaggagca ggagcgcaag caacttgccg agcagcagaa ggcccgccgc gatgaactca
     9721 tgcgcggcaa ctggaaaggc aagggcgaag tgctcaacgc catgcgcagc gtgattgccg
     9781 ccgagcaggc cgcagagaaa gccgccttga aggagaagca ccagaagcag cgcgagcagc
     9841 accggcagca gttcagaccc tatcccgacc tggagcagtg gcagcggatg cagaaaagcc
     9901 cggagcttgc cgagcagtgg cgacaccggg ccagcgagcc gcagcgcatc gagggcgacc
     9961 gcagcgagcc gccgacgccg cgcgacattc gcgcctatca gcctgaaatc gtgggccagc
    10021 aggtgcatta cagccggaag gaagaagggg ggcggggcgg gggtgtgtct ttcgtggaca
    10081 agggcaaaag catcgatgat cgagcgcgac aactcgccgg gaatgagtct ctagcgttgc
    10141 gtggtggttg tgatatactt gtatagcgtt ttcagaacag gagccgaaac atgcttgcaa
    10201 tccgactgcc cgccgaagtg gaaacccgcc ttgaagcact ggcgcaggcc acagggcgga
    10261 ccaagacttt ctatgcccgc gaagccatcc ttgagcactt ggatgacctc gaagatttgt
    10321 accttgcaga gcaacgcctg atcgacattc gcgcaggcaa aacccaaacc gtgccactcg
    10381 aagaagtgat gaaacgctat ggcatggaag gttgaactcg acccagccgc cgagcgcgag
    10441 ctaggcaaga tcgaccagca gaccgcccgc cgcatcctcg cttttttgca tggccgtgtc
    10501 gcccagctcg acgacccgcg cagcattggc gaagccctca aaggctccaa actgggagcc
    10561 ttctggaaat accgcgttgg ggattggcga atcatcgcca gcatcgagga cggtgctttg
    10621 cgcatcctcg ttatgcgcat cggcaatcgt aaggaggttt accgccaatg atcgaataca
    10681 gctaccagat cgacccgcgc ccctccgacc ttggcggcgg ctggcggttg cgcctgttgg
    10741 aaagcggcga ggaagtcggc ggcggagtgt tcccgttgtc cgagtacgcc acagcagaga
    10801 acgcagaaga agcggccacg tacgcctatg aggacgcctt ggccgaggct tcggcgtggc
    10861 tggcatcgag gggcgaaaat tgagcggcgc ggcaggggat tgcggccccg gcagcgccta
    10921 accacaactg tctgaaaagg agacaagc
//
