LOCUS       RSF1010                 8684 bp    DNA     circular BCT 30-NOV-2020
DEFINITION  Escherichia coli.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia; Escherichia
            coli.
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of RSF1010
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8684)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
     mobile_element  1..8684
                     /gene="RSF1010"
                     /product="Plasmid: IncQ-1RSF1010"
     CDS             1..840
                     /gene="repA"
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein RepA"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKS
                     MLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAV
                     ADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVI
                     GRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSA
                     EAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPR
                     GEA"
     CDS             827..1678
                     /gene="repC"
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein RepC"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
                     YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
                     AVTAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
                     LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
                     DPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDI
                     TRPKAAG"
     CDS             1986..2774
                     /gene="sul2"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydropteroate synthase"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLVRHPAIPTPRLFRPTQKSRVCAGAGRAQADGIPVSLDSYQPATQAYALSRGVAYL
                     NDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAAL
                     TGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRAL
                     TGRGPGVSGPRHSLQSLPPPQVELTSSAHTSRAPCATGWRYWRR"
     CDS             2858..3661
                     /gene="strA"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptomycin phosphotransferase StrA"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     CDS             3661..4497
                     /gene="strB"
                     /codon_start=1
                     /transl_table=11
                     /product="streptomycin phosphotransferase StrB"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     mobile_element  4469..5125
                     /gene="truncated ISCR2"
                     /product="Insertion sequence: truncated ISCR2"
     CDS             complement(4469..5008)
                     /gene="truncated tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="Truncated ISCR2 transposase"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRNPTLRLWPAIFSGEIDV
                     FPGDRRSRLISSMTSVAPA"
     repeat_region   5110..5125
                     /gene="terIS"
                     /product="terIS of ISCR2"
     repeat_region   5132..5208
                     /gene="iterons"
                     /product="RepC-binding sites; regulation of replication"
     CDS             complement(5562..5846)
                     /gene="mobC"
                     /codon_start=1
                     /transl_table=11
                     /product="Mobilization protein C"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRR
                     KVLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     misc_recomb     complement(5927..5964)
                     /gene="oriT"
                     /product="Origin region of transfer"
     CDS             6045..8174
                     /gene="mobA"
                     /codon_start=1
                     /transl_table=11
                     /product="Mobilization protein A"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAES
                     GHMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGA
                     ERLPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEAL
                     KPKAWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGR
                     GIRTDRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEH
                     GDTGRRSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERP
                     ERVAGVALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRT
                     LQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIR
                     PAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQ
                     IARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGP
                     ALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDD
                     LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSV
                     QLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6793..7206
                     /gene="mobB"
                     /codon_start=1
                     /transl_table=11
                     /product="Mobilization protein B"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQA
                     SEAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLA
                     SMMPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     CDS             7203..8174
                     /gene="repB"
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein B"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEV
                     LQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSP
                     KNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRA
                     GYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRR
                     TALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKP
                     GHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             8235..8447
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMA
                     SLNLDGCTLSLFREDKPFGPGKFLGD"
     CDS             8449..8655
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDE
                     YEALRECLEELRAAQGGGSDPASA"
BASE COUNT     1757 a   2627 c   2667 g   1633 t
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgaatgc catgtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag
     1861 atgtatgctc ttctgctcct gcagctaatg gatcaccgca aacaggttac tcgcctgggg
     1921 attccctttc gacccgagca tccgtatgat actcatgctc gattattatt attatagaag
     1981 cccccatgaa taaatcgctc atcattttcg gcatcgtcaa cataacctcg gacagtttct
     2041 ccgatggagg ccggtatctg gcgccagacg cagccattgc gcaggcgcgt aagctgatgg
     2101 ccgagggggc agatgtgatc gacctggtcc ggcatccagc aatcccgacg ccgcgcctgt
     2161 ttcgtccgac acagaaatcg cgcgtatgcg ccggtgctgg acgcgctcag gcagatggca
     2221 ttcccgtctc gctcgacagt tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg
     2281 tggcctatct caatgatatt cgcggttttc cagacgctgc gttctatccg caattggcga
     2341 aatcatctgc caaactcgtc gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg
     2401 aggcacccgc tggcgacatc atggatcaca ttgcggcgtt ctttgacgcg cgcatcgcgg
     2461 cgctgacggg tgccggtatc aaacgcaacc gccttgtcct tgatcccggc atggggtttt
     2521 ttctgggggc tgctcccgaa acctcgctct cggtgctggc gcggttcgat gaattgcggc
     2581 tgcgcttcga tttgccggtg cttctgtctg tttcgcgcaa atcctttctg cgcgcgctca
     2641 caggccgtgg tccgggggtg tcggggccgc gacactcgct gcagagcttg ccgccgccgc
     2701 aggtggagct gacttcatcc gcacacacga gccgcgcccc ttgcgcgacg ggctggcggt
     2761 attggcggcg ctgaaagaaa ccgcaagaat tcgttaactg cacattcggg atatttctct
     2821 atattcgcgc ttcatcagaa aactgaagga acctccattg aatcgaacta atattttttt
     2881 tggtgaatcg cattctgact ggttgcctgt cagaggcgga gaatctggtg attttgtttt
     2941 tcgacgtggt gacgggcatg ccttcgcgaa aatcgcacct gcttcccgcc gcggtgagct
     3001 cgctggagag cgtgaccgcc tcatttggct caaaggtcga ggtgtggctt gccccgaggt
     3061 catcaactgg caggaggaac aggagggtgc atgcttggtg ataacggcaa ttccgggagt
     3121 accggcggct gatctgtctg gagcggattt gctcaaagcg tggccgtcaa tggggcagca
     3181 acttggcgct gttcacagcc tatcggttga tcaatgtccg tttgagcgca ggctgtcgcg
     3241 aatgttcgga cgcgccgttg atgtggtgtc ccgcaatgcc gtcaatcccg acttcttacc
     3301 ggacgaggac aagagtacgc cgctgcacga tcttttggct cgtgtcgaac gagagctacc
     3361 ggtgcggctc gaccaagagc gcaccgatat ggttgtttgc catggtgatc cctgcatgcc
     3421 gaacttcatg gtggacccta aaactcttca atgcacgggt ctgatcgacc ttgggcggct
     3481 cggaacagca gatcgctatg ccgatttggc actcatgatt gctaacgccg aagagaactg
     3541 ggcagcgcca gatgaagcag agcgcgcctt cgctgtccta ttcaatgtat tggggatcga
     3601 agcccccgac cgcgaacgcc ttgccttcta tctgcgattg gaccctctga cttggggttg
     3661 atgttcatgc cgcctgtttt tcctgctcat tggcacgttt cgcaacctgt tctcattgcg
     3721 gacacctttt ccagcctcgt ttggaaagtt tcattgccag acgggactcc tgcaatcgtc
     3781 aagggattga aacctataga agacattgct gatgaactgc gcggggccga ctatctggta
     3841 tggcgcaatg ggaggggagc agtccggttg ctcggtcgtg agaacaatct gatgttgctc
     3901 gaatatgccg gggagcgaat gctctctcac atcgttgccg agcacggcga ctaccaggcg
     3961 accgaaattg cagcggaact aatggcgaag ctgtatgccg catctgagga acccctgcct
     4021 tctgcccttc tcccgatccg ggatcgcttt gcagctttgt ttcagcgggc gcgcgatgat
     4081 caaaacgcag gttgtcaaac tgactacgtc cacgcggcga ttatagccga tcaaatgatg
     4141 agcaatgcct cggaactgcg tgggctacat ggcgatctgc atcatgaaaa catcatgttc
     4201 tccagtcgcg gctggctggt gatagatccc gtcggtctgg tcggtgaagt gggctttggc
     4261 gccgccaata tgttctacga tccggctgac agagacgacc tttgtctcga tcctagacgc
     4321 attgcacaga tggcggacgc attctctcgt gcgctggacg tcgatccgcg tcgcctgctc
     4381 gaccaggcgt acgcttatgg gtgcctttcc gcagcttgga acgcggatgg agaagaggag
     4441 caacgcgatc tagctatcgc ggccgcgatc aagcaggtgc gacagacgtc atactagata
     4501 tcaagcgact tctcctatcc cctgggaaca catcaatctc accggagaat atcgctggcc
     4561 aaagccttag cgtaggattc cgccccttcc cgcaaacgac cccaaacagg aaacgcagct
     4621 gaaacgggaa gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca
     4681 gcaaggcggc actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct
     4741 tacagctgaa agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc
     4801 catgctccag ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca
     4861 attcctttcc ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct
     4921 gatagagaag ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc
     4981 tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata
     5041 cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga
     5101 cattgccagc cagtgcccgt cactcccgta ctaactgtca cgaacccctg caataactgt
     5161 cacgcccccc tgcaataact gtcacgaacc cctgcaataa ctgtcacgcc cccaaacctg
     5221 caaacccagc aggggcgggg gctggcgggg tgttggaaaa atccatccat gattatctaa
     5281 gaataatcca ctaggcgcgg ttatcagcgc ccttgtgggg cgctgctgcc cttgcccaat
     5341 atgcccggcc agaggccgga tagctggtct attcgctgcg ctaggctaca caccgcccca
     5401 ccgctgcgcg gcagggggaa aggcgggcaa agcccgctaa accccacacc aaaccccgca
     5461 gaaatacgct ggagcgcttt tagccgcttt agcggccttt ccccctaccc gaagggtggg
     5521 ggcgcgtgtg cagccccgca gggcctgtct cggtcgatca ttcagcccgg ctcatccttc
     5581 tggcgtggcg gcagaccgaa caaggcgcgg tcgtggtcgc gttcaaggta cgcatccatt
     5641 gccgccatga gccgatcctc cggccactcg ctgctgttca ccttggccaa aatcatggcc
     5701 cccaccagca ccttgcgcct tgtttcgttc ttgcgctctt gctgctgttc ccttgcccgc
     5761 acccgctgaa tttcggcatt gattcgcgct cgttgttctt cgagcttggc cagccgatcc
     5821 gccgccttgt tgctcccctt aaccatcttg acaccccatt gttaatgtgc tgtctcgtag
     5881 gctatcatgg aggcacagcg gcggcaatcc cgaccctact ttgtagggga gggcgcactt
     5941 accggtttct cttcgagaaa ctggcctaac ggccaccctt cgggcggtgc gctctccgag
     6001 ggccattgca tggagccgaa aagcaaaagc aacagcgagg cagcatggcg atttatcacc
     6061 ttacggcgaa aaccggcagc aggtcgggcg gccaatcggc cagggccaag gccgactaca
     6121 tccagcgcga aggcaagtat gcccgcgaca tggatgaagt cttgcacgcc gaatccgggc
     6181 acatgccgga gttcgtcgag cggcccgccg actactggga tgctgccgac ctgtatgaac
     6241 gcgccaatgg gcggctgttc aaggaggtcg aatttgccct gccggtcgag ctgaccctcg
     6301 accagcagaa ggcgctggcg tccgagttcg cccagcacct gaccggtgcc gagcgcctgc
     6361 cgtatacgct ggccatccat gccggtggcg gcgagaaccc gcactgccac ctgatgatct
     6421 ccgagcggat caatgacggc atcgagcggc ccgccgctca gtggttcaag cggtacaacg
     6481 gcaagacccc ggagaagggc ggggcacaga agaccgaagc gctcaagccc aaggcatggc
     6541 ttgagcagac ccgcgaggca tgggccgacc atgccaaccg ggcattagag cgggctggcc
     6601 acgacgcccg cattgaccac agaacacttg aggcgcaggg catcgagcgc ctgcccggtg
     6661 ttcacctggg gccgaacgtg gtggagatgg aaggccgggg catccgcacc gaccgggcag
     6721 acgtggccct gaacatcgac accgccaacg cccagatcat cgacttacag gaataccggg
     6781 aggcaataga ccatgaacgc aatcgacaga gtgaagaaat ccagaggcat caacgagtta
     6841 gcggagcaga tcgaaccgct ggcccagagc atggcgacac tggccgacga agcccggcag
     6901 gtcatgagcc agacccagca ggccagcgag gcgcaggcgg cggagtggct gaaagcccag
     6961 cgccagacag gggcggcatg ggtggagctg gccaaagagt tgcgggaggt agccgccgag
     7021 gtgagcagcg ccgcgcagag cgcccggagc gcgtcgcggg ggtggcactg gaagctatgg
     7081 ctaaccgtga tgctggcttc catgatgcct acggtggtgc tgctgatcgc atcgttgctc
     7141 ttgctcgacc tgacgccact gacaaccgag gacggctcga tctggctgcg cttggtggcc
     7201 cgatgaagaa cgacaggact ttgcaggcca taggccgaca gctcaaggcc atgggctgtg
     7261 agcgcttcga tatcggcgtc agggacgcca ccaccggcca gatgatgaac cgggaatggt
     7321 cagccgccga agtgctccag aacacgccat ggctcaagcg gatgaatgcc cagggcaatg
     7381 acgtgtatat caggcccgcc gagcaggagc ggcatggtct ggtgctggtg gacgacctca
     7441 gcgagtttga cctggatgac atgaaagccg agggccggga gcctgccctg gtagtggaaa
     7501 ccagcccgaa gaactatcag gcatgggtca aggtggccga cgccgcaggc ggtgaacttc
     7561 gggggcagat tgcccggacg ctggccagcg agtacgacgc cgacccggcc agcgccgaca
     7621 gccgccacta tggccgcttg gcgggcttca ccaaccgcaa ggacaagcac accacccgcg
     7681 ccggttatca gccgtgggtg ctgctgcgtg aatccaaggg caagaccgcc accgctggcc
     7741 cggcgctggt gcagcaggct ggccagcaga tcgagcaggc ccagcggcag caggagaagg
     7801 cccgcaggct ggccagcctc gaactgcccg agcggcagct tagccgccac cggcgcacgg
     7861 cgctggacga gtaccgcagc gagatggccg ggctggtcaa gcgcttcggt gatgacctca
     7921 gcaagtgcga ctttatcgcc gcgcagaagc tggccagccg gggccgcagt gccgaggaaa
     7981 tcggcaaggc catggccgag gccagcccag cgctggcaga gcgcaagccc ggccacgaag
     8041 cggattacat cgagcgcacc gtcagcaagg tcatgggtct gcccagcgtc cagcttgcgc
     8101 gggccgagct ggcacgggca ccggcacccc gccagcgagg catggacagg ggcgggccag
     8161 atttcagcat gtagtgcttg cgttggtact cacgcctgtt atactatgag tactcacgca
     8221 cagaaggggg ttttatggaa tacgaaaaaa gcgcttcagg gtcggtctac ctgatcaaaa
     8281 gtgacaaggg ctattggttg cccggtggct ttggttatac gtcaaacaag gccgaggctg
     8341 gccgcttttc agtcgctgat atggccagcc ttaaccttga cggctgcacc ttgtccttgt
     8401 tccgcgaaga caagcctttc ggccccggca agtttctcgg tgactgatat gaaagaccaa
     8461 aaggacaagc agaccggcga cctgctggcc agccctgacg ctgtacgcca agcgcgatat
     8521 gccgagcgca tgaaggccaa agggatgcgt cagcgcaagt tctggctgac cgacgacgaa
     8581 tacgaggcgc tgcgcgagtg cctggaagaa ctcagagcgg cgcagggcgg gggtagtgac
     8641 cccgccagcg cctaaccacc aactgcctgc aaaggaggca atca
//
