LOCUS       Tn7                    14067 bp    DNA     linear   BCT 15-OCT-2020
DEFINITION  Escherichia coli.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia; Escherichia
            coli.
REFERENCE   1  (bases 1 to 14067)
  AUTHORS   Zhou,D.
  TITLE     Compelete sequence of Tn7
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14067)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
     mobile_element  1..14067
                     /gene="Tn7"
                     /product="Unit transposon: Tn7"
     repeat_region   1..28
                     /gene="IRL_Tn7"
                     /product="Tn7 inverted repeat left"
     repeat_region   9..30
                     /product="TnsB-binding site 1"
     repeat_region   29..50
                     /product="TnsB-binding site 2"
     repeat_region   50..71
                     /product="TnsB-binding site 3"
     repeat_region   70..91
                     /product="TnsB-binding site 4"
     CDS             135..956
                     /gene="tnsA"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7 endonuclease"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     CDS             943..3051
                     /gene="tnsB"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7 transposase"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     CDS             3048..4715
                     /gene="tnsC"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7 transposition regulator"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     CDS             4718..6244
                     /gene="tnsD"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7 target-site selection protein"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     CDS             6353..7861
                     /gene="tnsE"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7 target-site selection protein"
                     /translation="MQERKWTRFTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQR
                     AKIIDFPNLSSFASVRNKDGAQSSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCR
                     SCLSSTALQQEFDVQYEVERDHLEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMD
                     SYESIFRHYQQNREIKNGVESWCFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLE
                     INAMLPSTTAISHASFQEKEAGDGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRV
                     IEAEPTWISFSRPSRIEKSRRARKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAA
                     DVGGKQDATNYNSIFANRFAAFDELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKD
                     GSPRVIKAVGVQRNGSEFVLLEVDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVK
                     SSLNWPNSLFDQLYGQDGHRGVNHPKGLGELQVSREDMEGWAERVVREQFTH"
     CDS             complement(8092..8469)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     CDS             complement(8523..8846)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MGIAYKLSCHRCGYESDLLYLGQGMAMLPEQIVGTCTCCDNLTT
                     ISINESYGVCSLCGAKDRVIFTSYQHEARPRNPCYLERSFKYQCPRCHAFSMEPPDLP
                     EMLWD"
     CDS             complement(8879..9250)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     CDS             complement(9311..9808)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     mobile_element  complement(9823..13131)
                     /gene="In2-4"
                     /product="Class 2 integron: In2-4"
     misc_recomb     complement(9823..9882)
                     /gene="attC_aadA1"
                     /product="attC site for aadA1"
     CDS             complement(9884..10672)
                     /gene="aadA1"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-adenylyltransferase"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_recomb     complement(10677..10735)
                     /gene="attC_sat2"
                     /product="attC site for sat2"
     CDS             complement(10730..11254)
                     /gene="sat2"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptothricin acetyltransferase"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     misc_recomb     complement(11260..11354)
                     /gene="attC_dfrA1"
                     /product="attC site for dfrA1"
     CDS             complement(11349..11822)
                     /gene="dfrA1"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydrofolate reductase"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     misc_recomb     complement(11836..11898)
                     /gene="attI2"
                     /product="attI2 site"
     regulatory      complement(11912..11939)
                     /gene="Pc2B"
                     /product="Promoter Pc2B"
     regulatory      complement(11912..11917)
                     /gene="-10 region_Pc2B"
                     /product="-10 region of Pc2B"
     regulatory      complement(11934..11939)
                     /gene="-35 region_Pc2B"
                     /product="-35 region of Pc2B"
     regulatory      complement(12020..12047)
                     /gene="Pc2A"
                     /product="Promoter Pc2A"
     regulatory      complement(12020..12025)
                     /gene="-10 region_Pc2A"
                     /product="-10 region of Pc2A"
     regulatory      complement(12042..12047)
                     /gene="-35 region_Pc2A"
                     /product="-35 region of Pc2A"
     misc_feature    12154..13131
                     /gene="intI2"
                     /product="IntI2 integrase"
     CDS             complement(13319..13558)
                     /codon_start=1
                     /transl_table=11
                     /product="Putative inner membrane proteins"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     repeat_region   13918..13939
                     /product="TnsB-binding site 5"
     repeat_region   13974..13995
                     /product="TnsB-binding site 6"
     repeat_region   14038..14059
                     /product="TnsB-binding site 7"
     repeat_region   14040..14067
                     /gene="IRR_Tn7"
                     /product="Tn7 inverted repeat right"
BASE COUNT     3906 a   2947 c   3326 g   3888 t
ORIGIN
        1 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
       61 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
      121 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
      181 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattccatgg ctaacagtac
      241 aagaagttcc ttcttcaggt cgttcccacc gtatttattc tcataagacg ggacgagtcc
      301 atcatttgct atctgactta gagcttgctg tttttctcag tcttgagtgg gagagcagcg
      361 tgctagatat acgcgagcag ttccccttat tacctagtga taccaggcag attgcaatag
      421 atagtggtat taagcatcct gttattcgtg gtgtagatca ggttatgtct actgattttt
      481 tagtggactg caaagatggt ccttttgagc agtttgctat tcaagtcaaa cctgcagcag
      541 ccttacaaga cgagcgtacc ttagaaaaac tagaactaga gcgtcgctat tggcagcaaa
      601 agcaaattcc ttggttcatt tttactgata aagaaataaa tcccgtagta aaagaaaata
      661 ttgaatggct ttattcagtg aaaacagaag aagtttctgc ggagctttta gcacaactat
      721 ccccattggc ccatatcctg caagaaaaag gagatgaaaa cattatcaat gtctgtaagc
      781 aggttgatat tgcttatgat ttggagttag gcaaaacatt gagtgagata cgagccttaa
      841 ccgcaaatgg ttttattaag ttcaatattt ataagtcttt cagggcaaat aagtgtgcag
      901 atctctgtat tagccaagta gtgaatatgg aggagttgcg ctatgtggca aattaatgag
      961 gttgtgctat ttgataatga tccgtatcgc attttggcta tagaggatgg ccaagttgtc
     1021 tggatgcaaa taagcgctga taaaggagtt ccacaagcta gggctgagtt gttgctaatg
     1081 cagtatttag atgaaggccg cttagttaga actgatgacc cttatgtaca tcttgattta
     1141 gaagagccgt ctgtagattc tgtcagcttc cagaagcgcg aggaggatta tcgaaaaatt
     1201 cttcctatta ttaatagtaa ggatcgtttc gaccctaaag tcagaagcga actcgttgag
     1261 catgtggtcc aagaacataa ggttactaag gctacagttt ataagttgtt acgccgttac
     1321 tggcagcgtg gtcaaacgcc taatgcatta attcctgact acaaaaacag cggtgcacca
     1381 ggggaaagac gttcagcgac aggaacagca aagattggcc gagccagaga atatggtaag
     1441 ggtgaaggaa ccaaggtaac gcccgagatt gaacgccttt ttaggttgac catagaaaag
     1501 cacctgttaa atcaaaaagg tacaaagacc accgttgcct atagacgatt tgtggacttg
     1561 tttgctcagt attttcctcg cattccccaa gaggattacc caacactacg tcagtttcgt
     1621 tatttttatg atcgagaata ccctaaagct cagcgcttaa agtctagagt taaagcaggg
     1681 gtatataaaa aagacgtacg acccttaagt agtacagcca cttctcaggc gttaggccct
     1741 gggagtcgtt atgagattga tgccacgatt gctgatattt atttagtgga tcatcatgat
     1801 cgccaaaaaa tcataggaag accaacgctt tacattgtga ttgatgtgtt tagtcggatg
     1861 atcacgggct tttatatcgg ctttgaaaat ccgtcttatg tggtggcgat gcaggctttt
     1921 gtaaatgctt gctctgacaa aacggccatt tgtgcccagc atgatattga gattagtagc
     1981 tcagactggc cgtgtgtagg tttgccagat gtgttgctag cggaccgtgg cgaattaatg
     2041 agtcatcagg tcgaagcctt agtttctagt tttaatgtgc gagtggaaag tgctccacct
     2101 agacgtggcg atgctaaagg catagtggaa agcactttta gaacactaca agccgagttt
     2161 aagtcctttg cacctggcat tgtagagggc agtcggatca aaagccatgg tgaaacagac
     2221 tataggttag atgcatctct gtcggtattt gagttcacac aaattatttt gcgtacgatc
     2281 ttattcagaa ataaccatct ggtgatggat aaatacgatc gagatgctga ttttcctaca
     2341 gatttaccgt ctattcctgt ccagctatgg caatggggta tgcagcatcg tacaggtagt
     2401 ttaagggctg tggagcaaga gcagttgcga gtagcgttac tgcctcgccg aaaggtctct
     2461 atttcttcat ttggcgttaa tttgtggggt ttgtattact cggggtcaga gattctgcgt
     2521 gagggttggt tgcagcggag cactgatata gctagacctc aacatttaga agcggcttat
     2581 gacccagtgc tggttgatac gatttatttg tttccgcaag ttggcagccg tgtattttgg
     2641 cgctgtaatc tgacggaacg tagtcggcag tttaaaggtc tctcattttg ggaggtttgg
     2701 gatatacaag cacaagaaaa acacaataaa gccaatgcga agcaggatga gttaactaaa
     2761 cgcagggagc ttgaggcgtt tattcagcaa accattcaga aagcgaataa gttaacgccc
     2821 agtactactg agcccaaatc aacacgcatt aagcagatta aaactaataa aaaagaagcc
     2881 gtgacctcgg agcgtaaaaa acgtgcggag catttgaagc caagctcttc aggtgatgag
     2941 gctaaagtta ttcctttcaa cgcagtggaa gcggatgatc aagaagatta cagcctaccc
     3001 acatacgtgc ctgaattatt tcaggatcca ccagaaaagg atgagtcatg agtgctaccc
     3061 ggattcaagc agtttatcgt gatacggggg tagaggctta tcgtgataat ccttttatcg
     3121 aggccttacc accattacaa gagtcagtga atagtgctgc atcactgaaa tcctctttac
     3181 agcttacttc ctctgacttg caaaagtccc gtgttatcag agctcatacc atttgtcgta
     3241 ttccagatga ctattttcag ccattaggta cgcatttgct actaagtgag cgtatttcgg
     3301 tcatgattcg aggtggctac gtaggcagaa atcctaaaac aggagattta caaaagcatt
     3361 tacaaaatgg ttatgagcgt gttcaaacgg gagagttgga gacatttcgc tttgaggagg
     3421 cacgatctac ggcacaaagc ttattgttaa ttggttgttc tggtagtggg aagacgacct
     3481 ctcttcatcg tattctagcc acgtatcctc aggtgattta ccatcgtgaa ctcaatgtag
     3541 agcaggtggt gtatttgaaa atagactgct cgcataatgg ttcgctaaaa gaaatctgct
     3601 tgaatttttt cagagcgttg gatcgagcct tgggctcgaa ctatgagcgt cgttatggct
     3661 taaaacgtca tggtatagaa accatgttgg ctttgatgtc gcaaatagcc aatgcacatg
     3721 ctttagggtt gttggttatt gatgaaattc agcatttaag ccgctctcgt tcgggtggat
     3781 ctcaagagat gctgaacttt tttgtgacga tggtgaatat tattggcgta ccagtgatgt
     3841 tgattggtac ccctaaagca cgagagattt ttgaggctga tttgcggtct gcacgtagag
     3901 gggcagggtt tggagctata ttctgggatc ctatacaaca aacgcaacgt ggaaagccca
     3961 atcaagagtg gatcgctttt acggataatc tttggcaatt acagctttta caacgcaaag
     4021 atgcgctgtt atcggatgag gtccgtgatg tgtggtatga gctaagccaa ggagtgatgg
     4081 acattgtagt aaaacttttt gtactcgctc agctccgtgc gctagcttta ggcaatgagc
     4141 gtattaccgc tggtttattg cggcaagtgt atcaagatga gttaaagcct gtgcacccca
     4201 tgctagaggc attacgctcg ggtatcccag aacgcattgc tcgttattct gatctagtcg
     4261 ttcccgagat tgataaacgg ttaatccaac ttcagctaga tatcgcagcg atacaagaac
     4321 aaacaccaga agaaaaagcc cttcaagagt tagataccga agatcagcgt catttatatc
     4381 tgatgctgaa agaggattac gattcaagcc tgttaattcc cactattaaa aaagcgttta
     4441 gccagaatcc aacgatgaca agacaaaagt tactgcctct tgttttgcag tggttgatgg
     4501 aaggcgaaac ggtagtgtca gaactagaaa agccctccaa gagtaaaaag gtttcggcta
     4561 taaaggtagt caagcccagc gactgggata gcttgcctga tacggattta cgttatatct
     4621 attcacaacg ccaacctgaa aaaaccatgc atgaacggtt aaaagggaaa ggggtaatag
     4681 tggatatggc gagcttattt aaacaagcag gttagccatg agaaactttc ctgttccgta
     4741 ctcgaatgag ctgatttata gcactattgc acgggcaggc gtttatcaag ggattgttag
     4801 tcctaagcag ctgttggatg aggtgtatgg caaccgcaag gtggtcgcta ccttaggtct
     4861 gccctcgcat ttaggtgtga tagcaagaca tctacatcaa acaggacgtt acgctgttca
     4921 gcagcttatt tatgagcata ccttattccc tttatatgct ccgtttgtag gcaaggagcg
     4981 ccgagacgaa gctattcggt taatggagta ccaagcgcaa ggtgcggtgc atttaatgct
     5041 aggagtcgct gcttctagag ttaagagcga taaccgcttt agatactgcc ctgattgcgt
     5101 tgctcttcag ctaaataggt atggggaagc cttttggcaa cgagattggt atttgcccgc
     5161 tttgccatat tgtccaaaac acggtgcttt agtcttcttt gatagagctg tagatgatca
     5221 ccgacatcaa ttttgggctt tgggtcatac tgagctgctt tcagactacc ccaaagactc
     5281 cctatctcaa ttaacagcac tagctgctta tatagcccct ctgttagatg ctccacgagc
     5341 gcaagagctt tccccaagcc ttgagcagtg gacgctgttt tatcagcgct tagcgcagga
     5401 tctagggcta accaaaagca agcacattcg tcatgacttg gtggcggaga gagtgaggca
     5461 gacttttagt gatgaggcac tagagaaact ggatttaaag ttggcagaga acaaggacac
     5521 gtgttggctg aaaagtatat tccgtaagca tagaaaagcc tttagttatt tacagcatag
     5581 tattgtgtgg caagccttat tgccaaaact aacggttata gaagcgctac agcaggcaag
     5641 tgctcttact gagcactcta taacgacaag acctgttagc cagtctgtgc aacctaactc
     5701 tgaagattta tctgttaagc ataaagactg gcagcaacta gtgcataaat accaaggaat
     5761 taaggcggca agacagtctt tagagggtgg ggtgctatac gcttggcttt accgacatga
     5821 cagggattgg ctagttcact ggaatcaaca gcatcaacaa gagcgtctgg cacccgcccc
     5881 tagagttgat tggaaccaaa gagatcgaat tgctgtacga caactattaa gaatcataaa
     5941 gcgtctagat agtagccttg atcacccaag agcgacatcg agctggctgt taaagcaaac
     6001 tcctaacgga acctctcttg caaaaaatct acagaaactg cctttggtag cgctttgctt
     6061 aaagcgttac tcagagagtg tggaagatta tcaaattaga cggattagcc aagcttttat
     6121 taagcttaaa caggaagatg ttgagcttag gcgctggcga ttattaagaa gtgcaacgtt
     6181 atctaaagag cggataactg aggaagcaca aagattcttg gaaatggttt atggggaaga
     6241 gtgagtggtt aggctagcta catttaatga caatgtgcag gttgtacata ttggtcattt
     6301 attccgtaac tcgggtcata aggagtggcg tatttttgtt tggtttaatc caatgcaaga
     6361 acggaaatgg actcgattta ctcatttgcc tttattaagt cgagctaagg tggttaacag
     6421 tacaacaaag caaataaata aggcggatcg tgtgattgag tttgaagcat cggatcttca
     6481 acgagccaaa ataatcgatt ttcctaatct ctcgtccttt gcttccgtac gcaacaagga
     6541 tggagcgcag agttcattta tttacgaagc tgaaacacca tatagcaaga ctcgttatca
     6601 catcccacag ttagagctag ctcggtcatt atttttaatt aactcctatt tctgtcgaag
     6661 ctgtttgagc agtaccgctt tacagcaaga gttcgacgtt cagtatgagg ttgagcgaga
     6721 tcatttagag ataaggatct tacccagttc atcgtttcct aaaggggcgt tagagcagtc
     6781 ggccgtagtg cagcttttgg tttggttgtt ttcggatcaa gatgttatgg attcgtatga
     6841 aagtattttt aggcactatc aacaaaatag agaaattaag aacggcgttg aaagctggtg
     6901 ctttagcttt gaccctccgc ccatgcaggg ttggaaatta catgtaaaag gacgttcttc
     6961 taacgaggat aaggattatt tagttgagga aatagtaggt ttagaaatca acgctatgct
     7021 tcctagcaca acagctatta gccatgcctc ttttcaggaa aaggaggcag gtgatggtag
     7081 tacgcagcac atagcggttt caacagagtc agttgttgat gatgagcatc tacagttgga
     7141 cgatgaggaa acagccaata tagacacaga cacacgagtc atagaggctg agccgacatg
     7201 gataagtttt agtagaccta gtcgaattga aaaatctcgc agggcaagaa aaagtagcca
     7261 aactatttta gaaaaagaag aagcaacaac aagtgaaaat agtaatttgg ttagtactga
     7321 tgagccacac ttaggtggtg tcctagcagc ggcagatgtg ggtgggaagc aggatgcaac
     7381 caattacaac tctatttttg ctaatcgatt tgctgctttt gatgagctac tttcaattct
     7441 aaaaactaaa tttgcatgtc gggtgctttt tgaagaaacc ttggttttgc caaaagttgg
     7501 gcgtagccga ttacatctgt gtaaagatgg ctcaccaaga gtgattaaag ccgttggggt
     7561 gcaacgtaat ggcagtgaat ttgtattgct agaggtggat gcatcggatg gggtgaaaat
     7621 gctttctacc aaagtgttga gtggcgttga tagcgaaaca tggcggaatg attttgaaaa
     7681 gatacggcgt ggagtggtga agagctcatt gaattggcca aatagtttgt ttgatcaatt
     7741 atatggacaa gacgggcata gaggggtgaa tcatccaaag gggttggggg agctgcaagt
     7801 atcgagagag gatatggaag ggtgggctga gagagtggtt agagagcaat ttacgcatta
     7861 aaggaatgac tgaaagagcc tgtaaaccct tttgtgtaag tgcttttgcc ggtcagttaa
     7921 aggtggccat ttaaacggtc accaaattcg atcataaaac ggttcatggc cggcttccag
     7981 ttgcggatcg gcatcgtcca tttcttggtc gccgcctgga tagccaggta caccaccttc
     8041 atcgctgatt cgtccgtagg gaacaccttg agttcatatt aatggaattt tctacaaata
     8101 gcctccgtgg ttttgagggg ggattacaga cgatccatag tagtaatcca atgagttctt
     8161 gagcgcggcg acatgtttgg acgccttggc aaaaattaga gcctgcttga agtgcaggcg
     8221 agcacgtgct tggacgattg atccattcgc ggtcaaaaac tcaatcttgg atgacagcgt
     8281 gtcaggaaat ccaatatcga agtcccgccc cgtgtcaaag tagggcattt catgatcaaa
     8341 ggacgaagct tcccaggcca cttccctaat attccagccg ctagaaacaa gatttcgtac
     8401 gacttcatca acttttttgc tttccaactt gtcacaaagt gcataggcaa gagctttggc
     8461 ggcagacacg tgcgtttcct cttaaacata ggtcgaacgg atacccgagg caaagcggag
     8521 cattagtccc aaagcatctc cggtagatcc ggaggctcca tactgaaggc atggcatctt
     8581 gggcattgat atttgaaact tctctctaga tagcagggat ttcttggcct tgcctcatgc
     8641 tgatagcttg taaatatcac acgatcctta gccccacaaa gtgagcatac tccgtagctt
     8701 tcatttatag aaatcgtcgt tagattgtcg cagcatgtgc acgttccgac tatttgctca
     8761 ggaagcatcg ccataccttg gcctaggtaa agaagatcag actcgtagcc acatcggtgg
     8821 caactgagct tgtacgcaat gcccatctgg ttgtctcacc tgaatcgtaa cgtcagagct
     8881 attcatccat ccattcggca tcaatgtatg ccactaagcc gaccagctga ccgtttggac
     8941 tgaagtatgt tgcctgcacc ccacttgaca tctcatcaat accaccgcac tgcgctaggc
     9001 gccggtccca cttgcccaca gaagcgtcag gatcggctga gttccccaag acccttgtga
     9061 gccatgcctc ctttaactca acctctcgaa tgattgcgtc ggccagctcc tccgcgttat
     9121 ctagttcgac atagtctgga attcggacgt acgttctgaa cacagctgga tacttacttg
     9181 ggcgattgtc atacatgaca tcaacaatgt acccgtttgt gtaaccgtct cttggaggtt
     9241 cgtatgacac tagtggttcc cctcagcttg cgactagatg ttgaggccta acattttatt
     9301 agagagcagg ctagttgctt agatacatga tcttcaggcc gttatctgtc agggcaagcg
     9361 aaaattggcc atttatgacg accaatgccc cgcagaagct cccatctttg ccgccataga
     9421 cgccgcgccc cccttttggg gtgtagaaca tccttttgcc agatgtggaa aagaagttcg
     9481 ttgtcccatt gttggcaatg acgtagtagc cggcgaaagt gcgagaccca tttgcgctat
     9541 atataagcct acgatttccg ttgcgactat tgtcgtaatt ggatgaacta ttatcgtagt
     9601 tgctctcaga gttgtcgtaa tttgatggac tattgtcgta attgcttatg gagttgtcgt
     9661 agttgcttgg agaaatgtcg tagttggatg gggagtagtc atagggaaga cgagcttcat
     9721 ccactaaaac aattggcagg tcagcaagtg cctgccccga tgccatcgca agtacgaggc
     9781 ttagaaccac cttcaacaga tcgcgcatag tcttccccag ctctctaacg cttgagttaa
     9841 gccgcgccgc gaagcggcgt cggcttgaac gaattgttag acattatttg ccgactacct
     9901 tggtgatctc gcctttcacg tagtgaacaa attcttccaa ctgatctgcg cgcgaggcca
     9961 agcgatcttc ttgtccaaga taagcctgcc tagcttcaag tatgacgggc tgatactggg
    10021 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta
    10081 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt
    10141 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag
    10201 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg
    10261 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa
    10321 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg
    10381 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc
    10441 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa
    10501 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca
    10561 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc
    10621 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc atgatgttta
    10681 actcctgaat taagccgcgc cgcgaagcgg tgtcggcttg aatgaattgt taggcgtcat
    10741 cctgtgctcc cgagaaccag taccagtaca tcgctgtttc gttcgagact tgaggtctag
    10801 ttttatacgt gaacaggtca atgccgccga gagtaaagcc acattttgcg tacaaattgc
    10861 aggcaggtac attgttcgtt tgtgtctcta atcgtatgcc aaggagctgt ctgcttagtg
    10921 cccacttttt cgcaaattcg atgagactgt gcgcgactcc tttgcctcgg tgcgtgtgcg
    10981 acacaacaat gtgttcgata gaggctagat cgttccatgt tgagttgagt tcaatcttcc
    11041 cgacaagctc ttggtcgatg aatgcgccat agcaagcaga gtcttcatca gagtcatcat
    11101 ccgagatgta atccttccgg taggggctca cacttctggt agatagttca aagccttggt
    11161 cggataggtg cacatcgaac acttcacgaa caatgaaatg gttctcagca tccaatgttt
    11221 ccgccacctg ctcagggatc accgaaatct tcatatgacg cctaacgcct ggcacagcgg
    11281 atcgcaaacc tggcgcggct tttggtacaa aaggcgtgac aggtttgcga atccgttgct
    11341 gccacttgtt aacccttttg ccagatttgg taactataat ttatgttaga ggcgaagtct
    11401 tgggtaaaaa ctggcctaaa attgctgggg atttcaggaa agtaaacatc accttccggc
    11461 tcgatgtcta ttgtagatat atgtagtgta tctacttgat cgatcaggct tttgtatatc
    11521 tccccaccac ctgaaacaat gacatgatcc gttattttct ttaggttggt taaagcatct
    11581 ttaattgatg gaaagatcaa tacgttctca ttgtcagatg taaaacttga acgtgttacg
    11641 accgcatact ttcggttggg taatgctccc attgattcaa aagtcttgcg tccaaccaac
    11701 agccattggt tataggtaat agctttaaac aggagctgtt cacctttggc actccatgga
    11761 atatcagggc cattcccgat aactccattc ttcgatatag ctaccattag tgatagtttc
    11821 acaattcttc ctcagaggtt aacattttat tagtaagcat gctcgttttg tcacccgctg
    11881 atgcttaccg ttaattaata taatcacttg tattaaatag actttcttta aaaatacaag
    11941 acactctgtt attacaaatc gtgcatgccg tctatcctgc ttgcacgatg cacattatct
    12001 cacccaaaga actcataaca ttttaattta tttagcatta aattaaaact ctaccggtaa
    12061 acaagcatct ctaggcgtaa aaaacaggca cttttttaaa tcccagctag aataggctgt
    12121 ataggcagac agttgcaaga caaggagcgt tttatgtcta acagtccatt tttaaattct
    12181 atacgcacgg atatgcgaca aaaaggttat gcgctgaaaa ctgaaaaaac ttacctgcac
    12241 tggattaagc gttttattct gtttcacaaa aaacgtcatc ctcagaccat gggcagtgaa
    12301 gaggtcaggc tgtttttatc cagcttagca aacagcagac atgtagccat aaacacgcag
    12361 aaaatcgctt taaatgccct agcttttttg tacaacaggt ttttacaaca gccgttgggc
    12421 gatattgatt atatccctgc aagcaagcct agacggctac cctctgttat ctctgcaaat
    12481 gaagtgcaac gcattttgca ggttatggat actcgcaacc aagttatttt tacgctgctg
    12541 tatggtgcag gtttgcgcat taatgaatgc ttgcgtttgc gggttaaaga ttttgatttt
    12601 gataatggct gcatcactgt gcatgacggt aagggtggga aaagcagaaa cagcctactg
    12661 cccacgcgcc taatcccagc aataaaataa ctcattgagc aagcgcggct tattcagcaa
    12721 gacgacaact tacaaggcgt agggccatcg ctgccttttg ctttagatca caaataccct
    12781 tctgcttatc gacaagcggc gtggatgttt gtctttccct ccagcacgct ctgcaaccac
    12841 ccgtataacg gcaaattatg ccgccatcat ctgcatgact ccgttgcgcg aaaggcattg
    12901 aaggcagccg tacaaaaagc aggcatcgtt agcaagcgtg tcacttgtca tacatttcgt
    12961 cactcgtttg ctacgcatct attacaagcg gggcgtgata ttcgcactgt gcaagaactc
    13021 ttagggcata acgatgttaa gaccacgcaa atctatacgc atgtgttggg tcagcatttt
    13081 gccggcacca ccagtcctgc ggatggactg atgctactta tcaatcagta aaagtagata
    13141 aatatcttta tgtagccctg agtagcccct gatacttttt atggtgaaaa agataaacaa
    13201 tcatagaggg gataaatatg agccagttct accattgttc agataagttt aagcaagata
    13261 ctgtcaatca cagtgaccat tcatggtcat tacacttcct ccagttgcca gacaatgcct
    13321 aacataaaaa cgcaaaagcc caaaagtcag agcttaggtg ttctaggcgt tttctaagcg
    13381 ctgaagaaat acgtcttacc gtagattgcg tgcgtttctg aagcacattt tccttataca
    13441 tggctttatc ccaggcagct tcatcaagct cttggagtaa caagccagta ataatgcgac
    13501 actctcgggt ttgtagcgag ccgctggtga gatcggagtt atagcggaaa ggggtcattt
    13561 gtaattattt gtgggtggag gatagttaac ttattattgt agttggaaga ggctgctttc
    13621 caaacattag cacctcgtaa tgcttgttac tgtatattaa tttatgctct tagcgcatgt
    13681 taatgggcta ttacaatgca taatgtccac atcaagataa ctgtggctta acacctgtac
    13741 aagcgaaaaa aatcaatcac ctgtccggaa atacgggatc actgtaaagt gttggtgagg
    13801 cagtctagga gggggattta atttgggata ctgatgaaag gattatgatc agttggatat
    13861 aggatcgtac taagctgagc ggcaggttag ctagattagt taaccagata agtgaaatct
    13921 agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct acacccagtt
    13981 cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc cacccctccc
    14041 agttcccaac tattttgtcc gcccaca
//
