LOCUS       Tn6889                  7707 bp    DNA     linear       29-SEP-2020
DEFINITION  Enterococcus casseliflavus.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Enterococcus casseliflavus
  ORGANISM  Enterococcus casseliflavus
            Unclassified.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn6889
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Enterococcus casseliflavus"
                     /mol_type="genomic DNA"
     repeat_region   1..25
                     /gene="IRL_ISEnca1"
     CDS             189..1451
                     /gene="tnpA"
                     /codon_start=1
                     /product="Transposase for ISEnca1"
                     /translation="MINKIDFTAKNLTSNAGLFLLLENAKSNEIFDLVEDDLVFDNDS
                     TNKIKMNHIKTMICGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFTFK
                     TTQMFRDINFKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYN
                     IQFAFCDELKAYVTGFVRSGNTYTANGASEMIKEIVANIKTDDLEILFRMDSGYFDEK
                     IIETIESLGCKYLIKAKSYSTLASQATNSSVVFVKGEEGRETTELFTKLDNWEKDRRF
                     VVSRVLKPEKERAQISLLEGSEYEYFFFVTNTTLLSEKVVISYEKRGNAENYIKEAKY
                     DMAVGHLLLKSFWANEAVFQMMMLSYNLFLLFKIDSLEPSEYRQQIKTFRLKYVFLAA
                     KIIKTARTVIMNLSENYPYKEVYEKCLV"
     repeat_region   1634..1658
                     /gene="IRR-1_ISEnca1"
     CDS             1661..2119
                     /gene="dinB"
                     /codon_start=1
                     /product="DinB family protein"
                     /translation="-
                     ENLEDTEAYWVYSSESLQVRKKNEVWIIDWPESESYEMGPMSIAWIMWHIIYWWSTAL
                     DYNFGSGSMKKDDVLWPGSIENAKEVIESLHEKWVSKLSELSDADFQMNQHAKWPLED
                     RNLADTALWLNAEFMKNAAEIGYARFLYGNSMKQK"
     misc_feature    2131..2526
                     /gene="truncated aph(2'')-Ie"
                     /product="Truncated APH(2'')-Ie/IVa family aminoglycoside
                     O-phosphotransferase"
     CDS             2637..3095
                     /gene="dinB"
                     /codon_start=1
                     /product="DinB family protein"
                     /translation="-
                     ENLEDTEAYWVYSSESLQVRKKNEVWIIDWPESESYEMGPMSIAWIMWHIIYWWSTAL
                     DYNFGSGSMKKDDVLWPGSIENAKEVIESLHEKWVSKLSELSDADFQMNQHAKWPLED
                     RNLADTALWLNAEFMKNAAEIGYARFLYGNSMKQK"
     CDS             3164..4069
                     /gene="aph(2'')-Ie"
                     /codon_start=1
                     /product="Aminoglycoside O-phosphotransferase APH(2'')-Ie"
                     /translation="MTTYTFDQVEEAIEQLYPDFTINTIEISGEGNDCIAYEINGNFI
                     FKFPKHSRASINLLNEVTVLKTIHNELSLPIPEVVFTGMPSEMCQMSFAGFTKIKGVP
                     LTPLLLKNLPKQSQDQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKL
                     LSRELKGHQMKKVDDFYRDILDNEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDF
                     GDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYMMKEKYWSFEKI
                     IYGKEYGYMDWYEEGLNEIRSIKIK"
     misc_feature    4246..4551
                     /product="Truncated tyrosine-type recombinase/integrase"
     CDS             4946..5254
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MQIKKIGGIIMFLILGIIAIVATVLNLYLYKVGKDYKLAMAMGL
                     SFTALTLVAGYSMISGWVKVKDWAALLDVVPTMTTVLWGLTIVSILLNIIPILLEQKK
                     "
     misc_feature    5731..6009
                     /product="Hypothetical protein (pseudogene)"
     CDS             6009..6149
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="-
                     IIFFELEKGIGKISQNNLKNKQKFSIENVLFLSKGYTLTVLLYTN"
     CDS             complement(6206..6331)
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MARVNTLAMLALAIFYFANFNAFADEKIKHIKIYADIFKTS"
     misc_feature    6746..7610
                     /gene="truncated repD"
                     /product="Truncated replication initiation protein,
                     topisomerase (pseudogene)"
     repeat_region   7683..7707
                     /gene="IRR-2_ISEnca1"
ORIGIN      
        1 ctgagattct acgtcagtac tttataaacc accctcaaag cattgataaa tatacgttcc
       61 ttcaaaattc agctttcacc cattgggtga aagaaaaatg ctcaaaaatg tgttgaatta
      121 tcagctttta tgagtcgata tatggtacaa taatagtaac aaaagcagca gaaaaagggg
      181 tttaaattat gattaataaa attgatttca cagctaagaa tctaacatca aatgcaggtc
      241 tttttcttct ccttgagaac gcaaaaagta atgagatttt tgatctagtt gaagatgacc
      301 tcgtatttga taatgactcc acaaataaaa tcaagatgaa tcatataaag acgatgatct
      361 gtggtcactt tattggtata gacaagctag aacgtctaaa gctccttcaa aatgatccac
      421 tcgttaatga atttgatatt tccgtaaaag aacctgaaac agtgtcacga tttttaggaa
      481 acttcacctt taagacaaca caaatgttta gagacattaa ttttaaagtc tttaaaaaac
      541 tgctcactaa aagtaaattg acatccatta cgattgatat tgatagtagt gtaatcaacg
      601 tagaaggtca tcaagaaggt gcatcaaaag gatataatcc caagaaacta ggaaaccgat
      661 gctacaatat ccagtttgca ttttgcgacg aattaaaagc ctacgtcacc ggatttgtaa
      721 gaagtggcaa tacttacaca gctaacggtg catctgaaat gatcaaagaa atagtagcca
      781 acatcaaaac agatgattta gagattttat ttcgaatgga tagtggctac tttgatgaaa
      841 aaattattga aacgatagaa tctcttggtt gcaaatattt aattaaagcc aaaagttatt
      901 ctacgcttgc atcccaagca acgaattcat cggttgtatt cgttaaaggt gaagaaggca
      961 gagaaactac agaactgttt acaaaattag ataactggga gaaagacaga aggtttgttg
     1021 tgtctcgcgt actgaaacca gaaaaagaaa gagcacaaat atcactttta gaaggctctg
     1081 agtacgaata ctttttcttt gtgacaaaca ctacattgct ttctgaaaaa gtagttatat
     1141 cctatgaaaa acgtggcaat gctgagaact atattaaaga agccaaatac gacatggcgg
     1201 tgggtcatct tttacttaaa tcattttggg caaatgaagc ggtgtttcaa atgatgatgc
     1261 tttcatataa cctgtttttg ttgttcaaga ttgattcctt agagccttca gaatacagac
     1321 agcaaataaa gacctttcgt ttgaagtatg tatttcttgc agccaaaatc attaaaaccg
     1381 caagaactgt aattatgaat ttgtcagaaa actatccgta caaggaagtg tatgaaaaat
     1441 gtctggtata ataaagatat catcttgaaa atcgagtgtt gctctgtgga taacttgcag
     1501 ggtttaataa gtatctttac tccaaagttg caatcaataa tttattgaaa aagattgaaa
     1561 ggtggtggta aataatgtta caatgtggga gtatcagttt aaattcttcg tgaaatagtg
     1621 ttcttttaag ctaataaaaa atgcacgtgg aatttaggat atagaaaatc ttgaggatac
     1681 agaagcatac tgggtatata gctcagaaag tcttcaggta cgaaagaaaa atgaggtatg
     1741 gatcatagac tggccggaaa gcgagagcta tgagatggga ccgatgagta tagcctggat
     1801 aatgtggcat atcatatatt ggtggtcaac tgcattagat tacaactttg gaagcggctc
     1861 aatgaaaaaa gatgatgttc tctggccggg tagtatagaa aatgctaaag aagtcataga
     1921 atccctgcac gagaaatggg tatctaaatt aagtgagtta tctgacgcgg actttcaaat
     1981 gaatcagcat gcaaaatggc cattagaaga taggaactta gcagatactg cactttggct
     2041 aaatgcagaa tttatgaaaa atgcagcaga gataggttat gcaagatttt tatatggaaa
     2101 tagcatgaaa caaaaatgaa ttaactataa ttgctaaatt cttatttaca gtcaatcaga
     2161 tttttgatta aagggagtag agaagacatg acaacttata ctttcgacca ggtagaagag
     2221 gcaatagagc agttatatcc tgattttact atcaatacaa tagagatttc aggagaaggc
     2281 aatgactgta ttgcatatga aataaacggg aattttattt ttaaatttcc aaagcattca
     2341 agagcttcga ttaatctctt gaatgaagta accgtactca aaacaatcca caatgaatta
     2401 tcactaccca ttcccgaggt ggtttttaca ggaatgccat cagaaatgtg ccaaatgtct
     2461 ttcgcaggtt ttacaaaaat taaaggagta cctttgacac ctcagttgca atcaataatt
     2521 tattgaaaaa gattgaaagg tggtggtaaa taatgttaca atgtgggagt atcagtttaa
     2581 attctcgtga aatagtgtct tttaagctaa taaaaaatgc acgtggaatt taggatatag
     2641 aaaatcttga ggatacagaa gcatactggg tatatagctc agaaagtctt caggtacgaa
     2701 agaaaaatga ggtatggatc atagactggc cggaaagcga gagctatgag atgggaccga
     2761 tgagtatagc ctggataatg tggcatatca tatattggtg gtcaactgca ttagattaca
     2821 actttggaag cggctcaatg aaaaaagatg atgttctctg gccgggtagt atagaaaatg
     2881 ctaaagaagt catagaatcc ctgcacgaga aatgggtatc taaattaagt gagttatctg
     2941 acgcggactt tcaaatgaat cagcatgcaa aatggccatt agaagatagg aacttagcag
     3001 atactgcact ttggctaaat gcagaattta tgaaaaatgc agcagagata ggttatgcaa
     3061 gatttttata tggaaatagc atgaaacaaa aatgaattaa ctataattgc taaattctta
     3121 tttacagtca atcagatttt tgattaaagg gagtagagaa gacatgacaa cttatacttt
     3181 cgaccaggta gaagaggcaa tagagcagtt atatcctgat tttactatca atacaataga
     3241 gatttcagga gaaggcaatg actgtattgc atatgaaata aacgggaatt ttatttttaa
     3301 atttccaaag cattcaagag cttcgattaa tctcttgaat gaagtaaccg tactcaaaac
     3361 aatccacaat gaattatcac tacccattcc cgaggtggtt tttacaggaa tgccatcaga
     3421 aatgtgccaa atgtctttcg caggttttac aaaaattaaa ggagtacctt tgacacctct
     3481 tctactcaaa aatctgccga agcaatctca agatcaggca gctaaggacc tggcccgatt
     3541 tttaagtgaa cttcacagca taaatatctc tggattcaaa agtaatctgg tattagattt
     3601 tcgagagaag ataaatgaag ataataaaaa aatcaaaaag ttactatcca gggaattaaa
     3661 gggtcaccag atgaagaaag tggatgattt ttacagggat attctagaca acgaaatcta
     3721 cttcaaatac tatccttgtc ttattcataa cgatttcagc agcgatcata ttttatttga
     3781 taccgaaaaa aataccattt gtggaataat cgattttgga gatgcagcta tttctgatcc
     3841 cgacaatgat tttataagtt tgatggaaga tgatgaagag tacggcatgg aattcgtatc
     3901 aaaaatattg aaccattaca aacataagga tataccgaca gttttggaaa aatatatgat
     3961 gaaagaaaaa tactggtcgt tcgaaaagat tatctatgga aaggaatatg gttatatgga
     4021 ttggtatgaa gagggattaa atgaaatcag aagcattaaa attaaataga tatttaatat
     4081 aaaggactgc taatgtactc atcaatagca atcctttttt agaatccgga atctttttta
     4141 ttttatttct aatgccgagg caaataaatc tacacaggaa tcgattatga gaaaacagag
     4201 aattataccg agcttcatct aagaagttat aaaaatgata tttatttgtc ttcgcaggat
     4261 tataagatca aacttaaaat cagaaacgaa aaaaaatata aatatgaaga attaataaat
     4321 aaaccgggta aatataaaat gcgtgttttt ccggtttttt gttccacaat acgggggttt
     4381 ttcgaggaaa tcacgtcttt tttctttaat ttcacgatat cggacatgcg taaaccgcta
     4441 ttaatgccga tcaaaaacaa gaaaacgtcg cgttcagcgt ttttgttgcg tctcaaacag
     4501 aataaaaagt cgttgatttc ttgctgcgtt cgtaatggtt gtacattata actcattgaa
     4561 atggtctctc ctctcacaaa acagatacat gatttcgttc attataccac gaaatcatgt
     4621 gttggacacc gatataaaaa aagaacccta tagatatagg attctggata cacgatttta
     4681 tacaatttca tgtatcataa attcttgttt aaaaaataaa aatttaaata tgttatcttt
     4741 aaacaaaata taacataagg agtgcacttt atatggcttt aactttagta tgtttcttgt
     4801 ttataattat tgttatatcc aagaataagg acaaatatca aaagtaaaca gaacattgtt
     4861 aaatctgagt tcagcttact tgattctatc tagtttaatg tattgatacg tcataatatc
     4921 tagaataaga tacttatgat actgaatgca aatcaaaaaa attggaggaa ttattatgtt
     4981 tctaatatta ggaattattg ctatagtagc aactgtttta aatctttatt tatataaagt
     5041 aggcaaagat tataaattag caatggcaat gggattgtca ttcacagcac tgacgcttgt
     5101 tgcaggatac agcatgatat ccggttgggt aaaggtaaaa gattgggctg ctttattaga
     5161 tgtagtacct actatgacaa cagtcctatg gggtttaacg attgtatcga ttttattaaa
     5221 tataattccg atccttttag agcaaaaaaa ataattgaaa ctaatcaact atctgataag
     5281 agtctactaa aatcaacact tactaggtat tgatctgatt gatttttcgc tgtctattat
     5341 aagtgtcgat taaaatattc aggtttcctt acttaaaaaa acgtgtatat attctcttga
     5401 gctaataagg ttttatatac acgactttaa gcaatttcac gtattgattt tgaaaataat
     5461 ctacaaaacc tagagtactt ttatataatt ttccagaaaa aattaattgc ataaataaaa
     5521 ataaaaaagc agagatcaaa aaaattgacc actacttttt tatttttttc tgtaaaaaaa
     5581 agagttaaaa agtcctaaaa ccgtacactt aatgtgtact ttttgtacac attaagtgta
     5641 catttgtgta catttgtgta cttaaaaacg ctgaaaccgt tgatacgaag acttttttaa
     5701 agattaaaaa aaacatcatt ttttgccaat ttgacgtctc tttttttatt taagattcgt
     5761 caggcacagc ctgacacgtc aacctttttg agcacagcga aaaaacaggt gacgtcggaa
     5821 tctctttatg ctcttaccaa gaactaccta aatctcattt tattttcgct accatttttt
     5881 tctgtttttg attttttttc aaatgagtgc aacgaatttt taaaattgcg gagcgatttt
     5941 tcacacattt ttttagcaca aacacgatat ttttttatcg tgtataagtg cgccctttta
     6001 aattttgagt gattatattt tttgagttag aaaaagggat tgggaaaatt tcccaaaata
     6061 atttaaaaaa taagcaaaaa ttttcgatag agaatgtgct atttttgtca aagggatata
     6121 ccttgactgt gctgctgtac acgaactaat ttttttgata taagttttgg aatttaaatc
     6181 actcttagaa gctcatattt gcgttttaag acgttttaaa tatatctgcg tatattttta
     6241 tgtgttttat cttttcgtcg gcaaatgcgt taaaatttgc gaaataaaat attgcaaggg
     6301 cgagcatagc gagtgtattt acccttgcca ttattttttg agtaaattag ctttgctgaa
     6361 cgacgaaaag gaaacacata acttttcaga ggtaaggggt ttttaagggg cgcttttagc
     6421 cccttagatt tatttgcttt aagatatatt tagttttaaa atagatttaa cgaaaaaaag
     6481 acataaaaaa agacgagcaa cgctaccaac gttctcgtcc ggaataacaa atcgcacttg
     6541 ttattcattc gaaacttagt atatcaattt tttctcttga atacaaaata aaaaaggacg
     6601 aatggataaa gcaaattttt ttgtaattcc atttgtcctt ttttcgttcc aaaaattatt
     6661 ttaggagcga taaaaaatga agaaaattga tgaaaaatta gctgcagaaa tctcacaaaa
     6721 aaaaccggcc gtctctaata gccggtaata gccggttagg agtgcattct gtgcacccac
     6781 aaaatcctaa attaagtttt gacgctatga caatagttgg aaatctcagt cgtgacaacg
     6841 ctcaaaagct atcgtcattt atgagtattg aacctcaaat aagactttgg gatatactac
     6901 aaacgaaatt taaagctaaa gcactgcaag aaaaagttta tatcgaatat gacaaagtga
     6961 aagcagatac ttgggacaga cgtaatatgc gtgttgaatt taatccaaat aaacttacac
     7021 atgaagaaat gatttggtta aaacagaaca tcatcgacta catggaagat gacggtttta
     7081 caagattaga tttagctttt gattttgaag atgatttgag tgattactat gcaatgactg
     7141 ataaagcagt taagaaaact attttttacg gtcgcaatgg taaaccagaa acaaagtatt
     7201 ttggtgtgag agatagcaat agatttatta gaatttacaa taaaaagcaa gaacgtaaag
     7261 acaatgcaga tgttgaagtt atgtctgaac acttatggcg tgtagaaatt gaacttaaaa
     7321 gagatatggt ggattattgg aatgattgtt ttaatgattt acatatcttg aaaccagatt
     7381 ggacttcgcc agaaaaatta aatgaacaag caatggttta catgttaatt catgaagaag
     7441 gcaaatgggg agaattgaat aagagaacta aatataaata taaaaaaata atcaaagaaa
     7501 tatctccaat tgatttaact gaaataatga aattgacttt acgagaaaac gaaaaacaat
     7561 tgcaaaagca gattgatttt tggcatcgtg aatttagatt ttgggagtga aaaaacaatg
     7621 aggactgaaa aagaaatttt aaatttagtt tctgaatttg cttatcaacg aagcaatgta
     7681 aaaattattg ctctcgaggg ttcaaga
//
