LOCUS       Tn6886                  6603 bp    DNA     linear       29-SEP-2020
DEFINITION  Uncultured bacterium.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Uncultured bacterium
  ORGANISM  Uncultured bacterium
            Unclassified.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn6886
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Uncultured bacterium"
                     /mol_type="genomic DNA"
     repeat_region   complement(1..18)
                     /gene="IRR_ISKpn12"
     CDS             complement(18..431)
                     /gene="tnpB"
                     /codon_start=1
                     /product="ISKpn12 transposase"
                     /translation="MIGRTKGGMNTKLHAVTDASGRPISFFITAGQVSDYTGAAALLD
                     ELPKAKWLLADRGYDADWYRDALQAKGITPCIPGRKSRKKTIKYDKRRYKRRNRIEIM
                     FGRLKDWRRVATRYDRCPMAFLSAIALAATVIFWL"
     CDS             complement(428..778)
                     /gene="tnpA"
                     /codon_start=1
                     /product="ISKpn12 transposase"
                     /translation="MSNLFWLTDEQMARLQPYFPKSHGRQRVDDRRVLSGIIFVNRNG
                     LRWRDAPKEYGPAKTLYNRWKRWGDKGVFIQMMEGLAVPEAAEHKTIMIDATYLKAHR
                     TASSLRVKKGARAA"
     repeat_region   complement(825..842)
                     /gene="IRL_ISKpn12"
     CDS             complement(842..961)
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MAKRLSASSRRPLRLRANSKQRRTKKLTRMSDRFGGCRV"
     repeat_region   1031..1043
                     /gene="truncated IRL_ISKpn11"
     CDS             1094..1426
                     /gene="tnpA"
                     /codon_start=1
                     /product="ISKpn11 transposase"
                     /translation="MPGTGDTMTRRPRRNHSPAFKAKVALAAIRGEQTLVELSQQFDV
                     HANQIKQWKDQLLDGATGVFGDEAKAEPAGPTVDVKTLHAKIGELTLENDFLAGALGK
                     AGLLGGKK"
     CDS             1423..2253
                     /gene="tnpB"
                     /codon_start=1
                     /product="ISKpn11 transposase"
                     /translation="MIDRTHKLSVARQARLLGFSRGSVYYSPRQVSDGDLDLMRRIDE
                     LHLDYPFAGSRMLQGLLRGEGTEVGRLHVATLMKKMGIEAIYRRPNTSKPAPGHKIYP
                     YLLRKLAVTRPNQVWAMDITYVPMARGFVYLCAVVDWFSRRVLSWRLSITMEADFCIE
                     AVEDALARYGKPEIFNTDQGSQFTSIDFTAVLKKAEIAISMDGKGAWRDNVFVERLWR
                     SIKYEEVYLHAYKTVSEARAGIGRYLAFYNSRRPHSSLDRQTPDQAYFNALAPMMVAA
                     "
     repeat_region   2302..2315
                     /gene="truncated IRR_ISKpn11"
     CDS             2350..3210
                     /gene="aacC2"
                     /codon_start=1
                     /product="Aminoglycoside N(3')-acetyltransferase"
                     /translation="MHTRKAITEAIRKLGVQTGDLLMVHASLKAIGPVEGGAETVVAA
                     LRSAVGPTGTVMGYASWDRSPYEETLNGARLDDKARRTWPPFDPATAGTYRGFGLLNQ
                     FLVQAPGARRSAHPDASMVAVGPLAETLTEPHELGHALGEGSPVERFVRLGGKVLLLG
                     APLNSVTALHYAEAVADIPNKRWVTYEMPMLGRNGEVAWKTASEYDSNGILDCFAIEG
                     KPDAVETIANAYVKLGRHREGVVGFAQCYLFDAQDIVTFGVTYLEKHFGATPIVPAHE
                     AAQRSCEPSG"
     CDS             3223..3765
                     /gene="tmrB"
                     /codon_start=1
                     /product="Tunicamycin resistance"
                     /translation="MIIWINGPFGAGKTTLAERLRDRRPKSLIFDPEEIGFVVKETVP
                     IPASGDYQDLPLWRGLTIAAVSEIRRNYSQDIIIPMTLVHPDYLTEILDGLRQIDDQL
                     LHIFLMLNEDLLRHRISNQTMHPDPNRNAEIREWRLANVARCLAARERLPSTTRVLDS
                     GAHTSDELAAMVLDRLDQRT"
     CDS             complement(3826..4008)
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MPERLDFNHLVDQLAGRRFVSLQGGGFGSWRLYAAVVLFRNGSR
                     PFASRKPTVRRYGLGE"
     CDS             4347..4538
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="-
                     VIGFIGVLLVVKPGYLPFQLGHLAAIGTGLATDAAIIILRRVGGKVKTTSMLGISARR
                     LQRG"
     CDS             4699..4938
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MSATDGHAFLIAGRFYRPPHQHGRSLLPADRSLQNIAILSYCGD
                     WAAGGEDLINEIRDLIFTHFILLMAGRRSHDLNKV"
     CDS             complement(5160..5711)
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MELQCSRLLARQDRQGLPVPLDRQDRGLWDRLLIRRGLEIVERA
                     CRMSSPTGWYLLQALISACHARAASFRDTNWREILARYDALFLLSPTYVVALNRAVAV
                     SWAMNPAAGMAVLKAIEDEWDVETYPLFHATRADFLSRLGFQDQAAAAYQAAAVLSTN
                     LPQKLFLVTRAEECLAGSRTDVG"
     repeat_region   complement(5762..5779)
                     /gene="IRR_ISKpn12"
     CDS             complement(5779..6192)
                     /gene="tnpB"
                     /codon_start=1
                     /product="ISKpn12 transposase"
                     /translation="MIGRTKGGMNTKLHAVTDASGRPISFFITAGQVSDYTGAAALLD
                     ELPKAKWLLADRGYDADWYRDALQAKGITPCIPGRKSRKKTIKYDKRRYKRRNRIEIM
                     FGRLKDWRRVATRYDRCPMAFLSAIALAATVIFWL"
     CDS             complement(6189..6539)
                     /gene="tnpA"
                     /codon_start=1
                     /product="ISKpn12 transposase"
                     /translation="MSNLFWLTDEQMARLQPYFPKSHGRQRVDDRRVLSGIIFVNRNG
                     LRWRDAPKEYGPAKTLYNRWKRWGDKGVFIQMMEGLAVPEAAEHKTIMIDATYLKAHR
                     TASSLRVKKGARAA"
     repeat_region   complement(6586..6603)
                     /gene="IRL_ISKpn12"
ORIGIN      
        1 gggtcaggac tcattgctca tagccagaag ataacggtcg cagcgagagc gatggcggag
       61 agaaaggcca ttgggcaccg gtcgtaacgc gtagcgacac gccgccagtc tttgagacgc
      121 ccgaacatga tctcgatccg gttgcgccgt ttgtagcggc gtttgtcgta tttgatggtc
      181 ttcttgcggg attttcgacc tggaatgcag ggagtgatcc ccttcgcctg taaagcgtca
      241 cgataccagt cggcatcata gccacggtcg gccagcaacc atttggcctt gggaagttca
      301 tcaagcaagg cggcagcacc ggtgtaatcg ctgacctggc ctgccgttat gaagaagctg
      361 atcgggcgac cactcgcatc cgttacggca tgaagcttgg tgttcatgcc gcctttcgtg
      421 cgtccaatca ggcggcccgc gccccctttt ttacccgcaa gctggaagcc gtgcggtggg
      481 ccttgagata ggtcgcatcg atcatgatgg tcttgtgctc tgcggcttca ggcacggcca
      541 gaccttccat catctggata aatacgccct tgtcgcccca gcgtttccag cggttataaa
      601 gcgtcttcgc cgggccatat tccttcggcg catcacgcca acgcagcccg ttgcggttga
      661 cgaagatgat gccgctcaga acgcgtcgat catcaacgcg ctggcggcca tggctcttgg
      721 ggaaataggg ctgaagacga gccatttgct cgtccgtcag ccaaaacaaa ttactcatca
      781 gtcgggctcc tatacgccca tctgaatcac agccgtcagt tcaaatcaat gggtcctgac
      841 cctaaacacg gcatccgccg aaacggtcgc tcatgcgcgt gagcttcttg gtccttcgct
      901 gcttgctatt cgcgcggagg cggagcgggc ggcgagacga cgcggataaa cgcttggcca
      961 aaaagccacc aacggcgacg gtcctccacc tcggtcaagc agcgagaggt agagtgatat
     1021 ctcgataggt agaggtggct cgggaaatct gcacagcggt gataagtgga atttctgcct
     1081 gacttcggct tagatgccgg gaacaggaga taccatgaca agacgaccgc gccggaacca
     1141 cagcccggct ttcaaggcaa aagtggcgct cgccgcaatc cgaggtgagc agacgctggt
     1201 ggaattgtcc cagcagttcg acgtgcatgc caaccagatc aaacagtgga aagaccagct
     1261 ccttgatggg gcgacaggcg ttttcggcga tgaagcgaag gcggaaccgg caggtccaac
     1321 cgtcgatgtc aaaacgctgc acgccaaaat aggcgaactg acattggaga atgatttttt
     1381 agccggagcg ctcggcaaag cgggattgct gggcggaaag aaatgatcga ccgaacacac
     1441 aaactgtcgg tcgcacgtca ggccaggctt ctcggcttca gccgtggcag tgtctactat
     1501 tctccacgtc aagtgtccga cggcgatctg gacctgatgc ggcggatcga cgaactgcac
     1561 ctcgactatc cgttcgccgg aagtcggatg ttgcaagggc ttttgagggg tgaagggacc
     1621 gaggtcgggc ggttgcacgt cgccacgctg atgaagaaga tgggcatcga ggcaatctac
     1681 cgtcgcccga acacctccaa accagcgcca ggtcacaaaa tctatcctta tctcctgcgc
     1741 aagctggcgg tcacccggcc caaccaggtc tgggcaatgg acataacgta tgtgccgatg
     1801 gcgcggggat tcgtctatct ctgcgccgtc gtggactggt ttagccggcg ggttctgtcc
     1861 tggcggctgt cgatcacgat ggaggcggac ttctgcatcg aagcggtcga ggacgcgctg
     1921 gcccgctatg gaaagcctga aatattcaac accgatcagg gttcgcagtt cacctccatc
     1981 gacttcacag ccgtgctgaa gaaggcggag atcgccatct cgatggatgg caagggcgcg
     2041 tggcgcgaca acgtcttcgt cgagcggctc tggcggtcga tcaaatacga agaggtctat
     2101 ctccacgcct acaagaccgt atccgaggct cgcgctggca tcggccgcta tctcgccttc
     2161 tacaatagcc gacgtccaca ttcatccctt gaccggcaga cgccggatca ggcctacttc
     2221 aacgcgctgg caccaatgat ggtggcggca taatcaaggc ggaaatccac ttagcaaaac
     2281 gcccaaaact gttcagacaa accgagccac ctctggtata gtgttttgca gtttagagga
     2341 gatatcgcga tgcatacgcg gaaggcaata acggaggcaa ttcgaaaact cggagtccaa
     2401 accggtgacc tgttgatggt gcatgcctca cttaaagcga ttggtccggt cgaaggagga
     2461 gcggagacgg tcgttgccgc gttacgctcc gcggttgggc cgactggcac tgtgatggga
     2521 tacgcgtcgt gggaccgatc accctacgag gagactctga atggcgctcg gttggatgac
     2581 aaagcccgcc gtacctggcc gccgttcgat cccgcaacgg ccgggactta ccgtgggttc
     2641 ggcctgctga atcaatttct ggttcaagcc cccggcgcgc ggcgcagcgc gcaccccgat
     2701 gcatcgatgg tcgcggttgg tccgctagct gaaacgctga cggagcctca cgaactcggt
     2761 cacgccttgg gggaagggtc gcccgtcgag cggttcgtcc gccttggcgg gaaggtcctg
     2821 ctgttgggtg cgccgctaaa ctccgttacc gcattgcact acgccgaggc ggttgcggat
     2881 atccccaaca aacgatgggt gacgtatgag atgccgatgc ttggaagaaa cggtgaagtc
     2941 gcctggaaaa cggcatcaga atacgattca aacggcattc tcgattgctt tgctatcgaa
     3001 ggaaagccgg atgcggtcga aactatagca aatgcttacg tgaagctcgg tcgccatcga
     3061 gaaggtgtcg tgggctttgc tcagtgctac ctgttcgacg cgcaggacat cgtgacgttc
     3121 ggcgtcacct atcttgagaa gcacttcgga gccactccga tcgtgccagc acacgaagcc
     3181 gcccagcgct cttgcgagcc ttccggttag aggccgtcga caatgataat ctggatcaac
     3241 ggacctttcg gcgccggaaa gacgactctc gctgagcggc tgcgcgatcg gcgtccgaaa
     3301 tcgctgatct ttgaccctga ggaaatcggg ttcgtggtga aagaaacggt ccccatacca
     3361 gcgagcggag actatcagga tctccccttg tggaggggac ttacgatcgc tgcggtcagc
     3421 gagattcgca ggaattactc gcaggacatc atcatcccaa tgacgctcgt gcacccggac
     3481 tatctgactg agatactcga cgggttaagg cagatcgacg atcagctgct gcacatcttt
     3541 ctgatgctta acgaggacct attgcgtcac cggatctcga accagaccat gcatcctgac
     3601 ccgaatcgga atgcggagat tcgagagtgg cgattagcga atgtcgcccg atgcttggcc
     3661 gcaagggaac ggcttccgtc cacaacccgt gttctcgata gtggtgcaca caccagcgat
     3721 gaacttgcag cgatggtgct cgacagactc gatcagcgca cctgatcgcc ttcgacgcct
     3781 gcgcaaaggg tagcgcgagg gtggtggtct cagtctgctt tgcgattatt ctcctaagcc
     3841 gtaccgcctg acggtcggtt tacgtgacgc gaatgggcga cttccgtttc ggaaaagcac
     3901 caccgcggca tagagacgcc agctgccgaa gcctccgcct tgaagggaca cgaaccgtcg
     3961 acccgcaagt tggtctacta agtgattgaa atccaaacgc tcaggcaagc ggggtgtcgg
     4021 ctgtagcgta acgttaaatt atagaaccta aagctccaaa tgctaaatgg tatcaagggg
     4081 ccaaatagcg gcggctacag tcgccgcctt taacggataa caacccgcaa cgactttcga
     4141 gcctgattgg cttcgcacgg cctagacacc agggttgcac atatttccag catacaatta
     4201 cactatagct ggatcaagcc caaggtgcac gccgtactac gacgcacttg atctaaagtt
     4261 ggcccttatc tcattgagac ggataaagaa actcctcgca aagctcgatt tgtggcttca
     4321 gcatcgcggt tcggcggtgg accgccgtgg tcatcggctt catcggagtg cttctcgtgg
     4381 tcaagccggg ctatctgccg ttccaattgg gtcatttggc tgccatcggc accgggctgg
     4441 ccacggacgc ggcaatcatc atcctcaggc gcgtcggcgg aaaggtgaag accaccagca
     4501 tgctgggcat cagtgcccgg cgcttgcagc gcggctgagg cacgacatat gaaggcgtag
     4561 ctggaaaccc ccgtcaggcc aaaattggcg acagggtcta gatcccttat tctaaagcgc
     4621 ggaccgaccc atagtcgcta ttggagtgtc tcaagccgca ggtcgtagaa acggccgagg
     4681 ctcctcaaat gatcgacgat gtcagcaaca gatggtcacg ctttccttat cgctggcaga
     4741 ttttatcgac cacctcacca acacggtcga agtcttctgc cagcagatcg ctctctgcaa
     4801 aacatagcaa tcttgtccta ctgcggtgat tgggcagccg gtggcgaaga tctcatcaac
     4861 gaaatccggg atctgatctt tacgcatttc atcctcctga tggcagggcg ccgatcgcat
     4921 gatctgaata aggtatgatc gtcatggctg actgaccgcg cgacgctttc agcggcgttg
     4981 aggcataacc ggagagccgc cgttcatacg tgatgcggcc tgcacatagg aaacatgtcc
     5041 tcaccgtaga agtgcagccg gatatgatgg cgccggcaca gccagcggac gttcaaaggc
     5101 tcgtcatacc tgtcatgatg cgcgtccacg tgcgcgcaat caccgtcaat cgactgatgt
     5161 tatccaacat ctgtccgaga ccctgccagg cattcctcag cgcgcgtgac caagaacaac
     5221 ttttgaggta ggttggtgct gagtacggcc gcagcctgat aggctgccgc agcttggtct
     5281 tggaaaccaa gacgcgaaag gaaatccgcc cgcgtcgcgt gaaaaagcgg gtaggtctcc
     5341 acgtcccact cgtcctcaat cgccttaagg accgccatcc ctgcagcggg gttcatggcc
     5401 cacgacacag cgactgctcg attgagggca actacgtatg tagggctcaa caggaagagc
     5461 gcgtcatatc tcgccagtat ctctcgccaa ttggtgtctc gaaagctcgc agcccgcgcg
     5521 tgacaagcag aaatcaacgc ttgcagaaga taccatccag tgggagacga catgcggcat
     5581 gctcgctcaa ctatctccag acctcgacgt atgagcaatc gatcccacaa gccacgatcc
     5641 tgacgatcga gcggcactgg caagccttga cgatcttgtc gggcaagcag ccgagagcac
     5701 tggagttcca tgagggctaa aagaccaagt acctcaggct cctctggctt gatatgcgct
     5761 agggtcagga ctcattgctc atagccagaa gataacggtc gcagcgagag cgatggcgga
     5821 gagaaaggcc attgggcacc ggtcgtaacg cgtagcgaca cgccgccagt ctttgagacg
     5881 cccgaacatg atctcgatcc ggttgcgccg tttgtagcgg cgtttgtcgt atttgatggt
     5941 cttcttgcgg gattttcgac ctggaatgca gggagtgatc cccttcgcct gtaaagcgtc
     6001 acgataccag tcggcatcat agccacggtc ggccagcaac catttggcct tgggaagttc
     6061 atcaagcaag gcggcagcac cggtgtaatc gctgacctgg cctgccgtta tgaagaagct
     6121 gatcgggcga ccactcgcat ccgttacggc atgaagcttg gtgttcatgc cgcctttcgt
     6181 gcgtccaatc aggcggcccg cgcccccttt tttacccgca agctggaagc cgtgcggtgg
     6241 gccttgagat aggtcgcatc gatcatgatg gtcttgtgct ctgcggcttc aggcacggcc
     6301 agaccttcca tcatctggat aaatacgccc ttgtcgcccc agcgtttcca gcggttataa
     6361 agcgtcttcg ccgggccata ttccttcggc gcatcacgcc aacgcagccc gttgcggttg
     6421 acgaagatga tgccgctcag aacgcgtcga tcatcaacgc gctggcggcc atggctcttg
     6481 gggaaatagg gctgaagacg agccatttgc tcgtccgtca gccaaaacaa attactcatc
     6541 agtcgggctc ctatacgccc atctgaatca cagccgtcag ttcaaatcaa tgggtcctga
     6601 ccc
//
