LOCUS       Tn6852                 13982 bp    DNA     linear       29-SEP-2020
DEFINITION  Pseudomonas aeruginosa.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Unclassified.
REFERENCE   1  (bases 1 to 13982)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn6852
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13982)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..13982
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
     repeat_region   1..18
                     /gene="attL_Tn6852"
     CDS             81..1370
                     /gene="int1"
                     /codon_start=1
                     /product="Site-specific integrase"
                     /translation="MATLERIHFVPHYLVRRENAVAYSPSSSRPSIEGLPQIFWADYA
                     PWREANLWAVERATTGDASLKTVASNMNGLLNYAKFLESRDLQWFEFPARKADRCLVL
                     YRGALIKMRDAGQISPSTASEYMRNCIMFYRWVRHRELLSPHVQLWREKPYVVKYFDR
                     VGFERTISGTTTDLSIPNRKRPGQTLEDGLLPVSETDRDAILDFAAENATPELYLMLA
                     LGFFTGMRLGSICDLKIQSLERAAPDPSAEGLLRLAVGPGAAPPVHTKFGVTGQVWIP
                     EALRDELLEYAKGWRRMEREAKASPENRDLLFLTRFGNAYGRRGTDQSSAVNVEMSEF
                     RKRGVKAELQVLRKFRFHQSRCTFGTELARLALSACADVAIVIATVSDALLHGPNSEA
                     TTFKYIRFVQALPIKQALSNSFMAAFSGIGAGARYDG"
     CDS             1363..3093
                     /gene="int2"
                     /codon_start=1
                     /product="Site-specific integrase"
                     /translation="MDSSEFDLTFPMLEYGATETPWDLRPLLFRGGAAAKVKHVGRQI
                     AQSELGSPLPERFELVTRLHEHMTDDLAGGGSRFSVQNKISALRRFFAWIDSENVNLS
                     LETAADTFIRWTDHLLQRHRVERNFSDGSLYDLTRLTATMLDRALDRQASLSKSTRIR
                     KPRGKGKVHTSKADKQSLQNTFAFGHLLADVCEALTWRGTMAPLPVCISLRTGQVLEL
                     WSGLQSPEKVAARRTRPQNQAQIEASLAARAAHDADRTLRTRFPIVNLRIESELLMFI
                     AQTGLNLQQAHTLRVEQFHYTSHLDGYQIRTYKNRREGEVLFEIFASYREWFERYLEW
                     RSEWFPNEPDGLLFPLIRSGGRILEEATQFTNVTRICRELGIPLVRPRKLRGTRINWL
                     LRESQNPQQVAELAQHTVQTLIRVYADPHPQIAMVEITRFHQQTDPSLSPPAPGRCVS
                     ATPEPVGTMPKNGPRPDCINAAGCLFCTQHRDIESEDHVWSLGSLRHLKSLELARYRP
                     PSSGKHLTTEHPALLVIDRLTAKLRFFEESSEVRRLWVEEARARISEGDYHPAWDGFI
                     QLAELRQRSA"
     CDS             3090..5129
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MTKNILSELGLGIDSPLAIPDAPNYRPPCWPPQRDWPVIIDAAG
                     QVVSRWGDAIWRLDAWAGKRLTLNFGDGPAKKYVAAIDPANADLLRTVIGWWLYGPNG
                     ARGYRGLKTRFDQMRRLFVLCTQEGILASELSHFPRVADLLPEVLQASRSGEFLALLH
                     ELYERRDALGFTLLDRAGLARLAAAMPDHQKLQTPYIPPRIWHYQITRLRECLDDFLA
                     HRGQVEECFRFCLAAYRHNSASLQGQRRPQSFYPFQWPSDGSNGKWTGRQYYGPFIDT
                     AHRFGMVELFRRWLGVSDDEIRIQTLSRYLNLVSRSGLCYLLNFSLMRVEEAWNLRAD
                     CLHIERDPQFGDIHVLRGRTTKTMSDSEALWVTSPSAQVAVEAMRVVADLRAECDSPP
                     GEASVPDDPAKRYLLDYCLEPWGTKFTKINRTIRPSIPSYAHVLQWFDKLFDPEQLRI
                     TPEDFALARLVTPTLTDEFAVGKIWPLAWHQLRRTGAVNMQASGLVSDASLQFQLKHV
                     ARAMSLYYGQNHSRVRLEEKAHTYYVRTMYETLGRQLQQLTSNRFVSPHGEKRKSEIV
                     RLISASDAKKAINLAKKGTVTHRPILLGICTSRTPCPYGGIDNIARCGGGDSPGETKP
                     CADVLYDPEQLDEVEVLEAVLDERLAAAEVDSPLRTSLEAQKRSVENYRHVIRQT"
     CDS             5110..5574
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MSSGKPESADPAKRMSAGEQYRAAFERLKSNKPERLPKGTPVSQ
                     NNVAKEAGSDPSALKKARFPLLIAEIQKYVEGHAEQRPPSVRQVSLLARRKNRGLRER
                     IEEITQQRDHLASLLGEADATILELYDRIADLERQLPASNVLPLNPRGHKKL"
     CDS             complement(5642..8332)
                     /codon_start=1
                     /product="AAA family ATPase"
                     /translation="MPLLDEIIGWAGGLRPWQQEALRRIFARAELTQDDIETILRMVR
                     EQEREDATTGGARPFTLDDVPGAGSGATVRLVGVSGLDQVNGFPSGRAFDLAPEGMTI
                     FFGHNGAGKSGYARVFKNACNARHRVEVLPDAFGAATPARPPSADFAILVDGTPETAR
                     WVQNGPAHLHLSSVSVYDAACANDYIDAEGTPAFQPYGLTHLTRLVLLQRDLQARIGT
                     ERNALALDTRQFEPLKGDTEVGRYIAKLGGDSDRAVLARLGTVGDDELRRLEFLKRTL
                     LESDPVPQALALERLATRLDQAQRRAEEVQRWVNDRAIARAKELITTEKTAHLAMQLA
                     QARLQDRDTLNAMELPVTSSPTATLLEGTGAELWQTMYRAAEAFSQQTAYPEHPFPHL
                     EPDAYCVLCQQPYSTDASERMRRFAAFVADSATADAQAATLARMNALGKVQAVDLNVL
                     DVPTRADVEERLPDLHAAITAAASVWTARHTWVSQALQTGNWSPESEPLPIEANLDSL
                     FAAKAASLRVDANTLRTSADPAVRLALTQELAELEARQRLANQLGAVERFVQDSQVHA
                     TLSRCHAALNPAAVSRKLTSLAATHVTEALAASMNAELKALGYKRRVQPDLSGRTELG
                     VTKVTLRLQEITAKASKVLSEGEQRALGMAMFLAELESLPHTSTVIFDDPSTSLDHVY
                     RRAIARRLVALAETRQVLVFTHDAVFLTELAMALQRADRSASYKTIGWDESPGLVSEG
                     LTWATMDTKARLADLESRAKALNVPVGSDPDDELERQIASGYSSLRGTVERAIREVFL
                     NNTVQPFSDVVSVEAFGAVIGHPAEEWEQLLAVYARACEATEAHDTPGERQLPLPARE
                     ELLGDIAQVLELIKNATKRRTAYENLRRERTAQRKKLFGG"
     CDS             8387..8638
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="-
                     ASASRCFAVFRLPAFPDRHPSGLSGRDATRAEFLHADQYQAALKSATGKRCLAVRDGE
                     SAEATTDASDVVSSPQVCAKLHSS"
     CDS             9060..10547
                     /codon_start=1
                     /product="SulP family inorganic anion transporter"
                     /translation="MLQTIKQNWFSNLRGDVLAGLVVALALIPEAIAFSIIAGVDPKV
                     GLYASFCIAVVIAFVGGRPGMISAATGAMALLMVTLVKNHGLEYLLAATLLCGVLQIA
                     AGYLKLGSLMRFVSRSVVTGFVNALAILIFMAQLPELTNVTWHVYAMTAAGLGIIYLF
                     PYVPKLGKVIPSPLVCILVLTAVAIYLGLDIRTVGDMGQLPDTLPIFLWPDVPLTFET
                     LAIIFPYSAALAVVGLLESMMTATIVDDLTDTTSDKNRECKGQGVANIASGLMGGMAG
                     CAMIGQSVINVKSGGRTRLSTLIAGVVLLLMVVFLSDWVSQIPMAALVAVMIMVSIGT
                     FSWDSLRNLRKFPLSTNIVMVATVVVVVFTHNLAYGVLVGVLLAAMFFANKVGHYMAI
                     SSELNEAADHRIYKVIGQVFFSSADKFVAAFDFKEAISKVTIDLSRAHFWDITAVAAL
                     DKVVVKLRREGTEVEVLGLNEASATIVDRFGVHDKPDAIDQLMGH"
     CDS             10562..11413
                     /gene="usp"
                     /codon_start=1
                     /product="Universal stress protein"
                     /translation="MTYVTACIDGSACAPAVCDYAAWASQRLNAPLTFLHVLDQRQYP
                     VSADLSGNIGLGSREHLLDELAALDEQRNKLALEQGLVMLAAAKERAIADGVSSPETR
                     QRHGDLLESLRELGNDIRLLVIGRQGESSDGAELHVGSQLESVIRIMRRPLLVTPTRF
                     KQPESAMLAFDGGATTRKGVEMLAASPLLKGLPIHLVMVGTETMENSAQMEAACSILA
                     SAGHKVHTAIRTGEVEPALHAYQEEHGVDLLVMGAYGHSRIRQFLVGSTTAHMLRTTT
                     TPLLLLR"
     CDS             11666..12907
                     /gene="chrA"
                     /codon_start=1
                     /product="Chromate efflux transporter"
                     /translation="MLAHLVTLDPTTMTDPLKTDRRPWAVFLIFLRLGLTSFGGPIAH
                     LGYFRDEFVVRRQWLSERSYADLVALCQFLPGPASSQVGIALGLSRSGYRGALAAWLG
                     FTLPSAIALIFFALGIASYGDAMPAGVLHGLKVVAVAVVAQAVWGMARNLCPDAPRIS
                     IMAAATCFVLLVPSAWGQVSVIILAAIVGLLLFKPQQGEAHDPLPISVRRRVGLFWLA
                     LFFALLLGLPLLTAVFPNQTLAMVDAFYRSGSLVFGGGHVVLPLLQAEVVPTDWVDND
                     AFLAGYGAAQAVPGPLFTFAAFLGASMNQAPTGWLGGLICLLAIFAPSFLLVVGALPF
                     WEHLRRNLRTQAALLGINAAVVGLLLAALYQPVWTSAIHGPKDFGLALVALVALMFWK
                     LPPWLVVLGSGILGGLLSIAL"
     CDS             12918..13031
                     /codon_start=1
                     /product="Helix-turn-helix transcriptional regulator"
                     /translation="-TDKDLYGGLIRLHILHHAAEAPIFGLAIIEELRHHG"
     CDS             13062..13652
                     /codon_start=1
                     /product="Class I SAM-dependent methyltransferase"
                     /translation="-
                     YTHKAADAVSWYQPHADSSLALIRQTGIAADAAIIDVGGGASTLVDDLLAAHYSDLTV
                     LDISAAALDVARHRLGGAASAVHWLEADITQVDFPEHRFALWHDRAVFHFLTDAADRA
                     RYLNAVGRAVKPGGFVVVGTFAEDGPEQCSGLPVRRYSADTLYDEFGAQFELLGKAEE
                     NHHTPFGTVQKFLYCYCRKLG"
     repeat_region   13965..13982
                     /gene="attR_Tn6852"
ORIGIN      
        1 taagagtatg ttgtatagtg cggacgccta agctattgag ttctatctat ggacattgtt
       61 aagcgtccca ggggcgttga atggcgacgc tagagcgcat tcatttcgtg ccccattacc
      121 tcgtgcgcag agagaacgcc gtcgcatact cccccagtag cagccggcca tccattgagg
      181 gcttgcccca aatcttttgg gcagactatg caccttggag ggaggcaaac ctgtgggcag
      241 tggagcgagc aacgactggt gacgcttctc ttaagaccgt tgccagtaac atgaatggcc
      301 ttctcaatta cgctaaattt cttgaatctc gtgatttgca gtggttcgag tttcccgctc
      361 gcaaagctga ccgctgcctg gtgctatacc gaggggcgtt gatcaagatg cgcgatgccg
      421 gccagatcag cccatccacg gcgtctgagt acatgcgcaa ttgcatcatg ttctatcggt
      481 gggtaaggca tagggagctt ctttctccac acgtccagct gtggcgggaa aagccctacg
      541 tcgttaaata cttcgaccgg gtggggttcg agcgaacgat cagcggaacc accaccgacc
      601 tcagcatccc gaaccgtaag cgcccaggcc aaacgcttga agatgggctg ttgccagtct
      661 cggagaccga tcgcgatgca attctggatt tcgccgcaga gaacgccacg cccgagctct
      721 acctcatgtt ggcccttgga ttttttaccg ggatgcggct cggcagcatc tgtgatctca
      781 agattcaatc tctggagcgc gccgcccccg acccttccgc tgaagggctt ctacgccttg
      841 cagttggtcc aggcgcagct ccgccagtgc acacgaaatt cggtgtgact ggccaggtct
      901 ggatacccga agcgttgcgt gatgaattgc tggaatatgc caaaggctgg cggaggatgg
      961 aacgagaggc gaaagcctcg cccgagaatc gtgacttgct gttcttgacc cgctttggta
     1021 acgcctatgg caggcgcggc accgatcagt catctgctgt gaatgttgag atgtccgagt
     1081 ttcgaaaacg gggcgtcaaa gcagaactac aggtgctccg taaattccgc ttccatcagt
     1141 cgcgctgcac cttcggtacc gaattggcac gtctggcgct atcggcttgt gccgatgtcg
     1201 ctattgtaat tgcgacggtc agcgacgcgt tgcttcacgg gcctaactct gaagccacca
     1261 ccttcaaata catccgattc gtacaggcac tgccgatcaa acaagccctc tcaaatagct
     1321 tcatggcggc atttagcggg atcggcgctg gggcgcggta cgatggatag ttccgaattc
     1381 gacctcactt tcccgatgct tgagtatggg gccactgaaa cgccctggga cctccggccg
     1441 ctgctgtttc gcggaggtgc agcagcaaag gtgaagcatg tgggccgcca gatcgcgcag
     1501 agcgaactcg gcagtccctt gccggaacgt ttcgagctgg tgacgcggct gcatgagcac
     1561 atgactgacg accttgctgg tggcggaagt cgcttctcgg tgcagaacaa gatcagtgca
     1621 ttgcgtcgat tctttgcctg gatcgattca gaaaacgtga atctcagtct cgagacggcg
     1681 gccgacacgt tcattcgttg gaccgatcac ttgctccagc gtcatcgagt cgaacgtaat
     1741 ttcagcgatg ggtccctgta cgatctcacc aggctcacgg cgacgatgct ggaccgagca
     1801 ctggaccgcc aagcaagcct gagcaagagc acacgtattc gcaagccgcg cggcaaaggc
     1861 aaggtacata cgagcaaagc ggacaagcag agtctgcaaa acaccttcgc attcggacac
     1921 cttctagctg acgtatgcga ggcattgacc tggaggggga caatggcccc cctcccagtg
     1981 tgtatttcgt tacgcaccgg ccaagtgctg gaactatggt cgggattgca gagccccgag
     2041 aaagtagcgg cccgccgtac caggccgcaa aaccaagcgc aaatcgaagc atccctggcg
     2101 gcgcgtgccg cccacgatgc agatcggaca ctgcggaccc gtttccccat cgtcaattta
     2161 cggatcgaga gtgaattgct gatgttcatc gcccagaccg gtctgaacct ccagcaagca
     2221 cataccctgc gggttgagca gtttcattac accagccact tagacggcta ccagatacgt
     2281 acctacaaaa atcgacggga aggagaagtg ctattcgaga tttttgccag ttatcgagag
     2341 tggttcgagc gctatctcga atggcgttcc gaatggtttc ccaatgagcc ggatggtttg
     2401 ctctttccgc ttatacgtag cgggggacga atcctagaag aggcgacgca gttcacgaac
     2461 gttacgcgca tctgccgcga gcttggcata ccgcttgtga gaccgcgaaa actgcgcggg
     2521 acgcgcatca actggttgct tcgggagtcc cagaatcccc aacaggttgc ggagttggcc
     2581 cagcatacgg tgcaaacgct gatacgcgta tatgccgacc cgcacccgca gatcgctatg
     2641 gtggaaatta cgcgcttcca tcagcaaacc gatccgtccc tttcacctcc tgctcctggt
     2701 agatgtgtat cggccacacc tgaaccagtg ggcacgatgc cgaagaatgg tccgcgaccc
     2761 gactgcatca atgctgccgg ttgcctgttc tgtacccagc accgggatat cgaaagtgag
     2821 gatcatgtgt ggtctctcgg tagcttgcgc cacctcaaat cgcttgaact ggcacgctac
     2881 cgcccaccca gttcgggtaa gcacctgacg acagagcatc cggccctgct ggtgatcgat
     2941 cggctgacgg ccaaactgcg cttcttcgaa gaaagtagcg aggtccgcag gctttgggtc
     3001 gaagaagcca gagcgcgcat aagtgaaggt gactaccacc cggcctggga tggcttcatc
     3061 caattggcag aactgcgaca aagatcagca tgacgaaaaa tattctttcc gaacttggcc
     3121 tcggcatcga ctcgccccta gcgattcctg atgcgccgaa ctaccgtccg ccgtgctggc
     3181 caccgcagcg tgattggccg gtcatcatag atgccgctgg ccaggtggtg agccgttggg
     3241 gcgatgccat ttggcggctc gacgcgtggg cgggaaaacg gctcactctc aatttcggcg
     3301 atggcccagc taagaaatat gtcgccgcga tcgatcctgc gaacgccgat ttattgcgga
     3361 ccgtaatcgg ctggtggcta tacggcccca atggagcccg tggataccga ggcctgaaaa
     3421 cgcgcttcga ccagatgcgt cggctgttcg tcttgtgcac tcaagaaggc attctcgcat
     3481 ccgaactgag tcattttccg cgcgttgccg accttctccc ggaggtgctc caggcttccc
     3541 ggtccggtga gtttttggca ttgctgcacg aactgtatga gcggcgggat gcgctggggt
     3601 tcaccttgtt ggatcgcgca gggttggcac gcctcgcagc ggccatgccc gaccaccaga
     3661 agctccagac cccctatatc ccgccccgca tttggcacta tcaaatcaca cgcttgcgtg
     3721 aatgcctgga tgactttttg gcgcatcgag gccaagtcga ggagtgcttc cgcttctgtc
     3781 tggctgccta tcggcacaac tctgccagtc tccaaggaca acgcaggcca caatcgttct
     3841 atccgtttca gtggccgtcg gatggatcaa acggaaagtg gactgggcgg cagtactacg
     3901 gcccattcat cgacacagcg catcggtttg gcatggtgga actatttcgt cgctggctgg
     3961 gagtgagcga cgacgaaatt cgcattcaaa ctctgagtcg ctacctcaac ctcgtgagcc
     4021 gatctggact gtgctatctg ctgaatttca gcctcatgcg agtcgaagag gcttggaacc
     4081 tgcgcgccga ttgcttgcac atagaacgtg atccgcagtt tggcgacatc catgtattgc
     4141 gcggacgaac gaccaagacg atgtcggatt ctgaggcact ttgggtgacg tccccgtcag
     4201 ctcaggtggc agttgaagcc atgcgtgtgg tcgcagacct ccgcgcagaa tgcgattctc
     4261 cccccggcga ggcctcggtg ccggatgatc cggccaagcg ctatctgctt gattattgcc
     4321 ttgaaccgtg gggaacgaag ttcaccaaga tcaaccgcac gattcggcca tcgattccga
     4381 gctatgccca tgtgcttcag tggttcgaca agctgttcga ccctgagcaa ttgcgcatta
     4441 cgccggaaga tttcgcattg gctcggttgg tgacgccgac gctgaccgac gagtttgcgg
     4501 tcggcaaaat ctggccgttg gcctggcatc aactccgacg aaccggcgca gtgaatatgc
     4561 aggcatccgg gctggtcagc gacgcttcat tgcagtttca gctaaagcac gtcgccagag
     4621 cgatgagtct ctactacggg caaaaccatt ccagggtacg gctggaagag aaggcccata
     4681 cctactacgt ccgcaccatg tacgaaactt tgggtaggca gctgcaacag ctgacgagca
     4741 atcgattcgt cagcccccac ggcgagaagc gaaaatctga gattgtccgc ctgatctcag
     4801 cctctgatgc taagaaagcg atcaatctgg ctaagaaggg gacggtcact catcgaccga
     4861 ttttgctggg catctgcacg agccgtaccc cctgtcccta tgggggcatc gacaatatcg
     4921 ctcgctgcgg agggggcgat tcccccggag aaaccaaacc atgtgcggac gttctctatg
     4981 accccgagca actcgacgaa gtcgaagtgt tggaggcggt attggatgag cgcctggccg
     5041 ctgccgaggt agatagtccg ctaaggactt cgttggaagc ccagaaacgt agtgtggaga
     5101 attatcgcca tgtcatccgg caaacctgaa tccgctgacc cggctaagcg aatgagtgcc
     5161 ggcgagcagt atcgcgcagc gttcgagcgg ctgaagagca acaagcccga acgactgccg
     5221 aaggggacgc ccgtaagtca gaacaacgtt gccaaggaag ccggcagcga cccctctgca
     5281 ctgaaaaaag ctcgtttccc cctactgatc gctgagatcc aaaagtacgt ggaaggacac
     5341 gctgagcaac gtccgccatc agtgcgccaa gtcagcttat tagcccgcag aaaaaatcgt
     5401 ggacttcgag aacggatcga ggaaatcacg cagcagcgtg accacctagc cagcctactc
     5461 ggtgaggccg acgccaccat tcttgagctg tacgaccgta ttgcggactt ggagcgccaa
     5521 ctgccggcct ccaatgtgct tcctcttaat ccgcgaggcc acaaaaaact ttgaaacgga
     5581 gagctcgcaa tattcgaaag aaacacagcc atgcccgacc agcccggcat gaaacgtcgg
     5641 gctagccgcc gaagagcttc ttgcgttgcg cggtccgctc ccggcgtaga ttctcgtagg
     5701 cagttcggcg tttagtcgcg tttttgatta attccaggac ctgggcgata tcgccgagca
     5761 actcctcgcg cgcgggaagc ggtagttggc gctcgcctgg ggtgtcgtgg gcctcggtag
     5821 cttcgcaggc ccgcgcatac actgccagca actgctccca ctcctctgcg gggtggccaa
     5881 tgacagcacc aaatgcttct acgctcacca catcgctgaa cggctggacg gtattgttca
     5941 aaaaaacctc gcggatggcg cgctcgacag tgcctcggag gcttgagtat cccgatgcaa
     6001 tttgccgctc aagctcatca tccgggtcgc tgccgaccgg aacgttcagc gctttcgcac
     6061 gactttcgag atcggccagg cgggccttgg tgtccatcgt cgcccacgtc agcccctcgc
     6121 tcactagtcc cggcgactcg tcccaaccga tggtcttgta gctggccgag cgatcagcac
     6181 gctgaagcgc catcgcgagc tcggtcagga acaccgcatc atgggtgaac accagcactt
     6241 ggcgggtctc agccagcgct accaaacgcc gagctatggc ccgacgatat acgtgatcca
     6301 gcgacgtaga cggatcgtcg aaaataacgg tcgaagtatg tggcagggat tcgagctcgg
     6361 cgaggaacat agccatcccc aacgcgcgct gctcgccctc tgacagcacc ttggaggctt
     6421 tcgccgtgat ttcctgcagg cgcagcgtga ccttggtcac ccccagctct gtgcgcccgc
     6481 tcaggtcggg ttgcacccgt cgcttatagc ccagggcttt caactcagca ttcatggaag
     6541 cggccagagc ctcggtcaca tgcgtcgccg ccagggacgt cagtttgcgt gacactgccg
     6601 ctgggttgag ggcggcatgg cagcggctca atgtggcatg gacctgactg tcctgaacaa
     6661 agcgctccac cgcgccaagc tggttggcga ggcgttgtcg ggcttctagc tctgccagtt
     6721 cttgggtcag cgccaagcgt acagcgggat cggcagaggt ccgcagggta ttggcgtcga
     6781 ctcggagcga agcagctttg gcggcgaaga gactatcgag gtttgcttcg ataggaagcg
     6841 gttctgactc gggggaccag tttccagttt gcagggcttg gctaacccaa gtatgccggg
     6901 cggtccagac cgatgccgct gcggtgatgg cagcgtgtag gtcgggcagt cgctcctcca
     6961 cgtcagcccg tgttggcaca tccagaacgt ttagatcgac agcctgcacc ttccctagag
     7021 cgttcatgcg cgcaagcgtg gcggcttgcg catccgccgt ggcgctgtcg gcgacaaatg
     7081 ccgcgaaacg ccgcatgcgc tccgaggcat ccgttgaata aggttgctgg cagagtacgc
     7141 agtaagcgtc cggttccagg tgcgggaagg gatgttcagg gtatgccgtc tgctgagaga
     7201 aagcctcagc ggctcggtac atcgtttgcc agagttcagc tcctgtgcct tccagcagcg
     7261 tggcggtggg tgaggacgtt accggcaact ccatggcgtt cagtgtgtct cgatcctgaa
     7321 gacgcgcttg cgccagttgc atggcgagat gcgcggtttt ttccgttgta atcagttcct
     7381 ttgcccgtgc tatggcacga tcattcaccc agcgctggac ttcctcggct cgacgctgcg
     7441 cctggtccaa gcgtgtcgcc aaacgttcca aggccagcgc ctgcggcaca ggatcgcttt
     7501 ccagtaatgt ccgcttcaga aactccagtc gccggagttc atcgtcaccg accgtcccta
     7561 atcgagcaag cacggcgcgg tccgagtccc cacctaattt ggcgatgtag cggccgactt
     7621 cggtgtcacc cttcagcggt tcgaattgcc gggtatccag agccaaggca ttgcgttcgg
     7681 tcccaatccg tgcctgtaaa tcacgctgaa gtaggaccag gcgcgtcaga tgagtgagtc
     7741 cgtagggctg aaatgcaggg gtcccttccg cgtcgatgta gtcattggca caggccgcgt
     7801 cgtagaccga gaccgaactc aaatgcaaat gggccggccc gttctggacc caacgtgcgg
     7861 tttccggagt gccatccacc aaaatggcga aatctgctga tggcggtcta gctggggttg
     7921 ccgccccaaa agcgtcgggc aggacctcca cccgatgccg agcgttgcag gcattcttga
     7981 agacgcgggc gtagccggat ttgccggcgc cgttatgacc aaagaaaatg gtcatgccct
     8041 ccggtgcgag gtcaaaggca cgaccggacg ggaagccgtt tacctggtct agccccgaga
     8101 cacccaccaa ccgcacggtt gcgccacttc cggcgcctgg tacgtcgtcc aacgtgaatg
     8161 gccgtgctcc ccccgtggtt gcgtcttccc gttcttgctc gcgaaccatt cgcagaatgg
     8221 tctcgatgtc atcttgggtt agttcggccc gggcaaatat ccggcgcagt gcttcctgct
     8281 gccagggtct gagtccgccg gcccacccaa taatctcgtc caacaatggc atatccgtct
     8341 cctttccaga tccgccgatt aaaggccgcg cccagttgga tggcaagtgg ccagcgcctc
     8401 gaggtgcttc gccgtgttcc gtttgcctgc cttccctgac cgccacccta gtggcttgtc
     8461 cggtcgtgac gctacacggg cagaatttct gcatgctgac cagtatcaag ccgctctaaa
     8521 gtccgctacc ggaaagcgct gtttagcggt gagagacggg gaaagtgcgg aagctactac
     8581 cgacgcaagc gatgtggtga gcagtcctca ggtatgtgcg aaattgcact cctcttgacc
     8641 accaatttgg gttggcgttg accacggatt gcgttcaact tgaccagggc gtgtcactgt
     8701 cacgaccgtc agagaacagc caagagcagt tatcgccata gcgcaatttt caggctctaa
     8761 cttttgccct agtgtcgcaa gactgggctc accgtgatat ggtctgcgct ctttcctgct
     8821 ctgactgctc aatacctgac agcatgatcg tctcgaaaat ctctgcctgg ctgcctacgc
     8881 cgccataacg catagccccc tccctgagtc agctcggctt accgactgat tcgtcttgct
     8941 gcgcctgtag caaatctgcc gttccacacg gcgccacgtg actcctacaa cttgaacctg
     9001 acgcttcctg cacgccgtcc tggcatcgtg ctgtcgcgtc cattgccttg gatttactga
     9061 tgttgcagac aatcaaacag aattggtttt cgaacctgag aggcgatgtg ctcgccggtc
     9121 tcgtcgtagc gcttgcgctg attccggagg ccatcgcctt ttccatcatc gcgggtgtcg
     9181 atcccaaggt cggcctgtat gcgtcgttct gtatcgccgt tgtgatcgct ttcgttggcg
     9241 gtcgacccgg catgatttcg gcggcgaccg gggcaatggc gctgctgatg gtgaccctgg
     9301 tcaagaacca tggcctggag tacctgctgg cagccacgct gctgtgtggc gtgcttcaga
     9361 tcgccgccgg gtacctgaag ctcggctcgt tgatgcgctt cgtgtctcgt tcggtggtga
     9421 ccggatttgt caacgcgctg gcgattctga tcttcatggc acagctacct gaactgacca
     9481 atgtcacctg gcacgtttac gccatgacgg ctgcgggcct cggtatcatc tacctgtttc
     9541 cctatgtacc caagctcggc aaggtgattc cctcaccgtt ggtgtgcatc ctggtcttga
     9601 ccgcagttgc catctatctc gggctggata tccgcaccgt cggcgacatg ggccaactgc
     9661 cggatacgct ccccatcttc ctgtggcctg acgtgccact gaccttcgag accctggcga
     9721 tcatcttccc ttactcggca gcactggctg tggttggtct gctggagtcg atgatgaccg
     9781 cgaccatcgt cgatgacctg accgatacca ccagcgacaa gaaccgcgag tgcaaaggcc
     9841 aaggcgtggc caacatcgct tcgggcctca tgggcggcat ggccggttgc gccatgatcg
     9901 gacagtcggt gatcaacgtg aaatccggtg gccgaacccg cctgtccacc ttgatcgcgg
     9961 gcgtcgtgct gctattgatg gtggtgtttc tcagcgactg ggtcagccag attcccatgg
    10021 ccgcgctggt ggcggtgatg atcatggtgt ccatcggtac cttcagctgg gactcgctgc
    10081 gcaatctgcg caaattcccg ctttccacca acatcgtgat ggtggcaacc gtggtcgtgg
    10141 tggtcttcac ccacaacctc gcctatggcg ttctggttgg ggttctgctg gctgcgatgt
    10201 tcttcgccaa caaggtcggt cattacatgg cgatcagctc agagttgaat gaagccgctg
    10261 atcatcgaat ttataaggtg attggccaag tgttcttcag ctcggccgac aagttcgttg
    10321 cggcgttcga tttcaaggaa gcgattagca aggtgaccat cgacctgagt cgcgctcact
    10381 tctgggatat cactgccgtc gctgctctgg acaaggtggt ggtcaagctg cgccgcgaag
    10441 gcaccgaggt cgaggtgctg ggtctgaatg aggccagcgc cactatcgtc gaccgttttg
    10501 gtgtgcacga caaacccgat gccatcgacc aactcatggg ccactgacag ggagaacaac
    10561 gatgacctac gttaccgctt gcattgacgg ctccgcctgc gcccctgctg tttgcgacta
    10621 tgcggcctgg gccagccagc gcctgaatgc cccgctcacc ttcctgcacg tattggatca
    10681 acgccagtat ccggtgtccg ccgatttgag cggcaacatc ggtttgggca gtcgagagca
    10741 tctgctggat gaactcgccg cgttggacga gcaacgcaac aagctggctc tggaacaagg
    10801 cctcgtcatg ctcgctgccg cgaaggagcg ggccatcgcc gacggcgtga gttcgccaga
    10861 aaccaggcag cgccatggcg atcttctgga aagcctgcgc gagcttggaa atgacatccg
    10921 cctgctggtg atcggtcgcc agggcgagtc cagtgacggt gccgaactgc acgtcggcag
    10981 ccagttggaa agtgtcattc gcatcatgcg ccgcccttta ctggtgacgc cgacccgctt
    11041 caagcagccg gaaagcgcga tgctggcctt cgatggcggc gccaccacgc gcaagggagt
    11101 agaaatgctg gctgcaagcc ctttgctgaa aggactgcct atccatctgg tgatggtcgg
    11161 aaccgagacg atggagaact cggcgcagat ggaggcggcc tgttcaatac tggcctcggc
    11221 agggcacaag gtacatacgg ccatccgaac tggggaggtt gagcccgcac tgcatgccta
    11281 ccaagaagag cacggtgttg atctgctcgt gatgggcgcc tacgggcatt ctcgaatccg
    11341 gcaattcctg gtggggagca ccactgccca catgctgcgc actactacca ctccgttgct
    11401 tctgttgcgc taggtcggtg ccggggagcg cgcccgaagg cgcgggccaa tttccctcat
    11461 ttgctgcctg ttccgacagg cagctggtga cggcttttta ctgcttctca accacagaaa
    11521 atggttccga ctgttcagga ctcggcagac aaatgggggg caaccgctgg aaatatttct
    11581 gtgtcttgta ggtggaggag tacaggccat gcgcagttgg cagcactgat ttcaatggtc
    11641 gtatttcgtt aacgattttc gattattgtt ggctcatctt gtcactcttg acccaacgac
    11701 catgaccgat cccctcaaga ccgaccgccg cccctgggct gtatttctga tattccttcg
    11761 cctagggctg acctccttcg gcggcccaat cgctcacctg ggctatttcc gcgatgagtt
    11821 cgtggtgcgc cgacaatggt tgagcgagcg cagctatgcg gatctggttg ccctttgcca
    11881 gtttctgcca gggccggcca gcagccaggt cggtatcgcg ctcggtctgt cgcgctctgg
    11941 ctatcgcggc gcgctggccg cgtggctggg cttcaccttg ccttccgcaa ttgccctgat
    12001 cttcttcgca ctcggcatcg ccagttatgg tgatgccatg cctgctggcg tgctgcacgg
    12061 tttgaaggtg gtggccgtgg cggtggtcgc ccaggcggtc tggggcatgg cgcgcaatct
    12121 gtgtcctgat gcaccgcgta tttccatcat ggcggcggcg acctgcttcg tgctgctcgt
    12181 accctctgcc tgggggcaag tgagtgtcat catccttgcc gccatcgtcg gcctgctgct
    12241 gttcaaacca cagcagggcg aggcccatga tccactaccg atcagcgtgc gacgtcgcgt
    12301 tggcttgttc tggctggccc tgttctttgc cctgctgctg gggctgccgt tgctgacagc
    12361 tgtgttcccg aaccagacat tggcgatggt ggatgccttc taccgctccg gttcgctggt
    12421 attcggtggc gggcatgtcg tactgccgct gttgcaagct gaggtcgtac caacagactg
    12481 ggtcgacaac gacgcattcc ttgcgggcta tggcgcggcg caggccgtgc cgggtccgct
    12541 gttcaccttc gcagcctttc tcggcgcctc gatgaaccaa gcacccactg gctggcttgg
    12601 cggcctgatc tgccttctgg cgatctttgc gccgtctttc ctgcttgtgg tgggggcctt
    12661 gccgttctgg gaacacctgc gccgtaacct gcgcacgcag gctgcactgc tgggcatcaa
    12721 tgcggccgtg gtcggcctgc tgctggccgc tctctaccag ccggtatgga ccagtgcgat
    12781 tcatggcccg aaagactttg gcctggccct cgtggcactg gtggcgttga tgttctggaa
    12841 gctgccgccg tggctggtcg tgctcggcag cggtatcctg ggcgggttgt tgagcattgc
    12901 cctgtgagag gcggcgagtg accgacaaag acctctatgg cgggttgata cgcctgcaca
    12961 tcctgcacca cgccgctgag gctcccattt tcgggctggc catcatcgaa gagttgcgac
    13021 accacggcta agcgatgcaa gacaagtccc actgggagtc cgtgtacacc cacaaggcag
    13081 ccgacgcggt gagctggtac cagccacatg cagacagctc cctcgcgctg attcgtcaaa
    13141 caggtatcgc tgcggatgct gcgatcatcg atgtcggcgg cggtgcttcg accttggtgg
    13201 acgacttgtt ggcagcgcat tacagcgacc tgaccgtgct cgatatttcg gccgccgcgc
    13261 tcgatgtggc gcgtcatcga ctgggaggcg ccgccagcgc cgtacattgg ctggaggcag
    13321 atatcaccca ggtggacttt cccgagcacc gcttcgcgct ctggcatgac cgcgctgtgt
    13381 tccattttct aaccgatgcc gcagacagag cgcgttatct caacgctgtg gggcgtgcgg
    13441 tcaagcctgg cggctttgtg gtcgtgggca ccttcgccga ggacggcccg gagcagtgca
    13501 gcggtttgcc tgtcagacga tacagcgccg acaccctgta cgatgagttt ggcgcgcagt
    13561 ttgagctgct ggggaaggct gaggaaaacc accatacgcc gtttgggacg gtgcagaagt
    13621 tcctctactg ctattgcaga aagctcggct gacgtctttc cggtgagacg acagtaggcg
    13681 gccctgttaa gtactcctat cgagcactgt ccccgttttt cggggcacga gaaacttgga
    13741 cgaatggcag ctcctggccg gtttctgcct gttgcgtctc tgctgcccac tggccaagtc
    13801 ggatgcacgc ggtggtcagt accaatgcaa ttgactggtc aaatgggtgc aattgcgcag
    13861 gtatggactt caagccccaa tttcctggaa aagaggttct ttctatggac atccgacatc
    13921 agagattgag tagcggaatg tctgagctga agtcatgtcc atggctgttc ttctttcctt
    13981 ta
//
