LOCUS       Tn6591                 15464 bp    DNA     linear       26-OCT-2020
DEFINITION  Shigella flexneri.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Unclassified.
REFERENCE   1  (bases 1 to 15464)
  AUTHORS   Zhou,D.
  TITLE     The complete sequence of Tn6591
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15464)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: aaaa v. bbbb
            Sequencing Technology :: PacBio
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
     mobile_element  1..15464
                     /gene="Tn6591"
                     /product="Relaxosome-encoding integrative and mobilizable
                     element: Tn6591"
     repeat_region   1..8
                     /gene="attL_Tn6591"
                     /product="Attachment site at the left end of Tn6591"
     CDS             317..1519
                     /gene="int"
                     /codon_start=1
                     /transl_table=11
                     /product="Site-specific integrase"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVAVTPAGSISFRYNYSIN
                     GRQETITFGRYGVGGITLAEARELLGDAKKMVAAGKSPAKEKARDKARVKDAETFGAW
                     AEKWLRGYQMADSTRDMRRSVYERELKPKFSNQKLVEITHEDLRALADAIVERGAPAT
                     AVHVREIVLQVFRWAIERGQKVENPAELVRPTSIARFEPRDRALTPEEIGLMYQYMER
                     VGTSPTNRAAAKLLLLTMVRKSELTNATWSEINFSEALWTIPKERMKRRNPHLVFLSQ
                     QALDIFIAMKTFAGGSDFVLPSRYDSDAPMSAATLNQVLTLTYKAAQKDGKSLTKFGP
                     HDLRRTASTLLHEAGYNTDWIEKCLAHEQKGVRAVYNKAEYREQRAAMLQDWADMIDE
                     WTSGGSKG"
     CDS             1667..1888
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /translation="MQNIKTLINRKKLLEMIPLSTRTIYNLEQRGDFPRRIALTSRNV
                     AWDLSEVEEWIEARKSSGDQAARPGPIEG"
     CDS             1893..2720
                     /gene="repA"
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein RepA"
                     /translation="MALDLMAAFTELPPPIDYVLPNMVAGTVGALVSPGGAGKSMLAL
                     QLAAQIAGGPDLLEIGEFPTGQVVYLPAEDPPAAIHHRLHALGAHLSAAERQAVADGL
                     LIEPLIGKCPNIMAASWFDALKRAAEGRRLMILDTLRRFHIEEENASGPMAQVVGHME
                     AIAADTGCSIVFLHHASKSAAMMGSGDQQQASRGSSVLVDNIRWQSYLSGMTQGEAEI
                     LGVDDCQRGYFVRFGVSKANYGAPFQELWFRRHDGGVLKPAVLERQCKVKRRQREEA"
     CDS             2707..3588
                     /gene="repC"
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein RepC"
                     /translation="MKKPKHDLTHVRHDPAHCLAPGLFRSLKRGDRKRCKLDVTYTFG
                     EDESMRFVGFEPLGADDMRLLQGIVALGGPNGILLTPEPTSETGRQLRLFLEPRFEAI
                     EQDGLVVRESLTKLLSETGMTDSGDNIKALKASLLRMSNVTILVTKGRRQAAFHLMSH
                     AFDETDGRLWVALNPRIAEAILGHRPYARIDMAEVRVLQTDPARLMHQRLCGWIDPGK
                     SGRVELDTLCGYVWPDEANAEAMKKRRQTARKALAELAAVGWVVNEYAKGKWEIKRPG
                     PTATAPVYRRNVPLLPS"
     misc_recomb     4282..4553
                     /gene="oriT"
                     /product="Origin region of transfer"
     CDS             4621..4809
                     /gene="stbC"
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid stability protein StbC"
                     /translation="MANVLIRNLPAEVHRALKVRAALNDRSTDAEIREILTAAVQPLE
                     LEEIGREVGLSESDKKTE"
     CDS             4950..5729
                     /gene="tivB8"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type type IV secretion,bitopic protein/inner
                     membrane complex component"
                     /translation="MKSKILAAKKTALAVALATGFITTTTAPVQAGIPVIDGGNLAQN
                     IMTAIESVAQTLKQIEQYQTQLQQYENQLQNTMAPAAYIWDQAQTTINRLIAAQNTLA
                     YYENQLGSLDRYLAKFQDVAYYRSSPCFNGSGGCTPAEKAAMEENRRLASESQKKAND
                     ALFQTVADQQKALKDDARTLERLQGAAQGATGQLQAIGYANQLASQQANQLLQIRTML
                     TAQHNAEAARIAAELDAEARGDARAEQMRTWTFRPSPADNY"
     CDS             5742..5978
                     /gene="eex"
                     /codon_start=1
                     /transl_table=11
                     /product="Entry exclusion protein"
                     /translation="MKKIFFTTAATLLLLAGCEQERMPEPNAATCAPDAFQAALNEMR
                     SEANREAFTEECRAFQKAKQMRQWEFKPSPKDDY"
     misc_feature    5989..7412
                     /gene="tivB6"
                     /product="P-type type IV secretion, polytopic
                     protein/inner membrane complex component (pseudogene)"
     CDS             7417..7665
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MTTSTYMAALAALDEAPREEVSQVFISPLGDQSTEVDAETETAA
                     FVSRPDPMRESETKPISIEVNGGRNERSAFAEAAGLYL"
     CDS             7729..7953
                     /codon_start=1
                     /transl_table=11
                     /product="Type I toxin-antitoxin system ptaRNA1 family
                     toxin"
                     /translation="MTTQHIIEPGQAVHQAAAILSSLEYINQAEARSLGPLAEAVANA
                     FMVVYYQAETGRATQADFQEAMNALRQACS"
     CDS             complement(7990..8193)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MTDPKNLENRLHEKADPARAVSTGRVRYTLAELLADAEASGAYP
                     LPPEEREWMDAPSVGREWPNSED"
     CDS             complement(8308..9375)
                     /gene="acr3"
                     /codon_start=1
                     /transl_table=11
                     /product="Arsenical-resistance protein ACR3"
                     /translation="MSAGAQAIAAKPRQAPMSGFERYLTLWVALCIVAGVALGQGLPD
                     VFQAIGRMEVAQVNLPVGLLIWVMIVPMLVKIDFGALHQVKEHWRGIGVTLFVNWAVK
                     PFSMALLGWLFIRQVFAPFLPADQLDSYIAGLILLAAAPCTAMVFVWSRLTNGDPLFT
                     LSQVALNDAIMIVAFAPIVGLLLGMSSISVPWDTLLISVVLYIIVPVILAQLLRRHLL
                     KQGQAAFERAMQKIGPWSMAALLLTLVLLFAFQGEAIIRQPLVIAMLAVPILIQVFFN
                     SGLAYWLNKRAGEKHSVACPSALIGASNFFELAVAAAISLFGLHSGAALATVVGVLVE
                     VPVMLLVVRVVNRSKSWYERG"
     CDS             complement(9372..9878)
                     /gene="arsC"
                     /codon_start=1
                     /transl_table=11
                     /product="Arsenic resistance transcriptional regulator
                     ArsC"
                     /translation="MTERIYNVLILCTGNSARSIMAEALINTMGQGRFRAYSAGSHPT
                     GKVNPFAVEKVESVNYPTENLRSKSWDEYATPDAPKMDFIITVCDNAAGEMCPVWPGQ
                     PISAHWGFEDPAAVEGTDAEKRRAFEQTFRHMMNRVRLFVNLPLKMLDQTAIKRELAN
                     IGKTEQEA"
     CDS             complement(9875..10642)
                     /gene="arsH"
                     /codon_start=1
                     /transl_table=11
                     /product="Arsenic resistance protein ArsH"
                     /translation="MRLRHLSDPDSLPALDKSFAIERPALGLAPDAPPVRILLLYGSL
                     RARSFSRLAVEEAARLLQFFGAETRIFDPSDLPLPDQVQSDDHPAVKELRALSEWSEG
                     QVWCSPERHGQITSVMKAQIDHLPLEMAGIRPTQGRTLAVMQVSGGSQSFNAVNTLRL
                     LGRWMRMFTIPNQSSIAKAFQEFDAAGRMKPSPYYDRIADVMEELVRFTALVRPHREA
                     LTDRYSERKAAGHVIDEATDLSSIAIAPQPLPESETS"
     CDS             complement(10639..10971)
                     /gene="arsR"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator, ArsR
                     family"
                     /translation="METINAVAALAAIAQESRLAVFRLLVQAGPAGMAAGKISEAAGI
                     PPSSLSFHLKELAHAGMVTSRQEGRFVIYEANFSTATNLVAFLTENCCGGQVCNLSCT
                     TEAGKVLA"
     CDS             11119..11856
                     /codon_start=1
                     /transl_table=11
                     /product="Resolvase"
                     /translation="MRRTKPVAAPMVARVYLRVSTDAQDLERQEAITTAAKAAGYYVA
                     GIYREKASGARADRPELLRMIGDLQPGEVVIAEKIDRISRLPLPEAERLVASIQAKGA
                     RLAVPGVVDLSDLAAEAQGVAKIVLEAVQIMLFRLALQMARDDYEDRRERQRQGIELA
                     RQAGRYKGRRADPKRRAQVVALRKSGYSINKTAELAGYSAAQVKRIWAEVSQAEAKQH
                     GAFVEDALTEADALAAVGQDERQEERA"
     CDS             11853..12077
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MKKPNQDDEPFFITEEIAAEMIAGGYEFELPPIPCTIRLRDVLE
                     RMTDAELALQPGEIADQERERCRRKPCSTS"
     mobile_element  complement(12171..15161)
                     /gene="ISCR2–sul2 unit"
                     /product="Putative resistance unit: ISCR2a##sul2 unit"
     mobile_element  complement(12171..14014)
                     /gene="ISCR2"
                     /product="Insertion sequence: ISCR2"
     repeat_region   12171..12186
                     /gene="terIS"
                     /product="ISCR2 terIS"
     CDS             12288..13781
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCR2 Transposase"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     CDS             complement(13957..14259)
                     /gene="glmM"
                     /codon_start=1
                     /transl_table=11
                     /product="Phosphoglucosamine mutase"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     repeat_region   13996..14014
                     /gene="oriIS"
                     /product="ISCR2 oriIS"
     CDS             complement(14346..15161)
                     /gene="sul2"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydropteroate synthase type-2"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     repeat_region   15457..15464
                     /gene="attR_Tn6591"
                     /product="'Attachment site at the right end of Tn6591"
BASE COUNT     3410 a   4178 c   4712 g   3164 t
ORIGIN
        1 gagtgggaat gattccgcgt ctggcacagc caggcacctg atggcatgaa atgccgctaa
       61 gcccgcgtaa ttgcgggctt ttttttgttt gtgtttggca cgatctggca acgggaggaa
      121 gcgccaagca ccgtttgagc atggcattaa agctggtatt atggtttggc gatgccatga
      181 ttgatgccga taccatgctg cgttcttttg tgtgttcatg gtattaatat tctataagtt
      241 actgtttcgt aagaaaaaat acgataaatt cgagattttt tcagcatggt atcggagacg
      301 aagaaggaca aggtacatgc tgaccgatac caagctgcgc aatctcaagc ccagggacaa
      361 actctacaaa gtgaatgacc gggaaggtct ctatgtggca gtgactccag ccggctccat
      421 ctcgttccgt tacaactact caatcaacgg tcggcaggag accatcacct ttgggcgtta
      481 tggtgtcggt ggcatcaccc tggccgaagc ccgcgagctg ttgggtgacg ccaagaagat
      541 ggttgcggcg ggcaagtcgc cggccaagga gaaagcccga gacaaggcgc gggtgaaaga
      601 tgcagagacg ttcggtgcct gggcggagaa gtggctgcgt ggctaccaga tggccgactc
      661 cacccgcgat atgcgccgct cggtttatga gcgtgagctg aagccgaaat tcagcaatca
      721 gaagctggtg gagatcaccc acgaagactt gcgggcgctg gctgatgcca ttgttgagcg
      781 aggcgcaccg gccaccgccg ttcatgtgcg tgaaatcgtg ttgcaggtat ttcgctgggc
      841 catcgagcgt gggcagaagg tcgaaaaccc ggcagaactg gtgcgcccaa caagcatcgc
      901 tcgattcgag ccacgtgacc gagcgttgac gccagaagaa attgggctga tgtaccagta
      961 catggagcga gtgggcacaa gcccaacaaa ccgtgcggcg gccaagctgt tattgctgac
     1021 gatggtgcgc aagagcgagc tgaccaatgc gacctggagc gagatcaatt tcagcgaagc
     1081 gttgtggacg attccaaagg agcggatgaa acgccgtaac ccgcacctgg tatttctgtc
     1141 ccagcaggct ctggatattt tcattgccat gaaaaccttt gccggtggtt ccgacttcgt
     1201 tttgccatca cggtacgact cggatgcgcc gatgagcgct gccacactta accaggtgct
     1261 gacgctgact tacaaggcgg cgcagaaaga tgggaagtca cttaccaagt tcggaccgca
     1321 tgatttgcgg cgcacagcca gcacgctgtt gcatgaggcc ggctacaaca ccgactggat
     1381 cgagaagtgc ctggcgcacg agcagaaggg cgtgagggct gtctacaaca aggctgagta
     1441 ccgcgagcag cgggcggcga tgttgcagga ttgggccgat atgattgatg agtggacttc
     1501 gggaggcagt aagggttgat gtttttgcta tctttgatag cattgaagtt gatgccgtga
     1561 atttataaat aaggccgcca ttggcatcgg gcaggtcaat taccgattag ctgcttcgcg
     1621 cagctactcg atgaatagca actattcatt tgagaggtat tgacccatgc aaaatataaa
     1681 gaccctcatc aacaggaaga agctcctgga gatgatcccg ctttcgacac gaacaattta
     1741 taacctggag cagcgagggg attttccacg ccgtatcgcg ttaaccagta gaaatgtcgc
     1801 ctgggatttg tcagaggtcg aagagtggat tgaggcacgt aaatcatcgg gtgatcaagc
     1861 tgcgcgacct ggccctatag agggctagtt atatggctct tgatcttatg gcggctttca
     1921 cggaattgcc gccacccatt gattatgttc tgcctaatat ggttgctggc actgttggtg
     1981 ctcttgtgtc gcctggtggg gctggtaaat ccatgttggc ccttcaattg gctgcacaga
     2041 ttgcaggcgg gcctgattta cttgaaatag gcgagtttcc caccgggcaa gtggtctatc
     2101 tgcctgctga agatccaccg gccgctattc accatcgtct gcacgccctt ggggcacacc
     2161 tcagcgcagc ggaacggcaa gccgtggctg atggtttgct cattgaaccc ttgatcggta
     2221 aatgcccaaa catcatggct gctagctggt tcgatgctct caaacgagcc gctgaaggtc
     2281 gtcgcttgat gatcttggac actttgcggc gcttccacat tgaggaggag aacgctagcg
     2341 ggccgatggc gcaggttgtt gggcacatgg aggccatagc cgctgataca ggctgctcca
     2401 ttgtgtttct gcaccacgcg agtaaaagcg cagccatgat ggggtcgggc gatcaacagc
     2461 aggccagtcg tggatcgtcc gtactggttg ataacatccg ttggcaatcg tacctatccg
     2521 gcatgacgca aggcgaggcc gagatactgg gggtcgatga ttgtcagcgt gggtattttg
     2581 tccgctttgg cgtcagcaag gccaactatg gcgcaccttt tcaagaactc tggtttagac
     2641 ggcacgacgg cggcgtgttg aagcctgctg tactggagcg gcagtgcaag gtgaaaagga
     2701 gacagcgtga agaagcctaa gcatgacctg acccacgtcc gacatgatcc cgcgcactgt
     2761 ttggcacctg gcctgttccg cagcctcaag cgtggcgatc gcaaacgctg caagctggac
     2821 gtgacctaca cctttggcga ggacgaatcc atgcgtttcg tcggattcga acctctcggg
     2881 gccgatgata tgcgtctttt gcaaggcatc gtggcccttg gcggcccgaa cggcatcttg
     2941 ctaaccccgg aaccgaccag tgagacgggg cgacagctac ggctattcct tgaaccccgt
     3001 ttcgaagcca ttgagcaaga cggcttggtg gttcgtgaga gcctgaccaa actgctctca
     3061 gaaacgggca tgacggatag cggcgacaac atcaaggcgc tcaaagccag cctgctgcgc
     3121 atgtcgaacg tcaccatcct tgtgacgaag ggacggcggc aagccgcgtt ccacctgatg
     3181 agtcatgctt ttgacgagac ggacggcagg ctatgggttg ccctgaatcc gcgtattgcc
     3241 gaagcgatcc tggggcatcg tccatatgcc cgtatcgaca tggcggaagt gcgggtgcta
     3301 cagactgatc cggcacggct gatgcaccaa cggctatgcg gctggatcga ccccggcaaa
     3361 tccgggcgcg tggaactgga cacgctttgc ggctatgtct ggccagatga agccaatgcc
     3421 gaagctatga aaaaacgccg tcagactgcc cggaaggcac tggccgaact tgccgccgtg
     3481 ggttgggtag tgaacgaata cgccaaggga aaatgggaga tcaagaggcc tggccccacg
     3541 gcaactgcac ccgtttaccg tcgtaacgtt cccttgttac cgtcgtaacg ctccccttct
     3601 gccgtcgtaa cgttccccgt cccaatttgg aaaacccaga cggtgcgctg ctttgcgggc
     3661 agtttgggaa atccatccaa gattatctaa agataatcca tcatgcgcgg tgcagctcgc
     3721 gccttgaggg cgcgttctga ccttgccaat aaagccctgg cgggctttat caatcggcca
     3781 taggccgatg gtttaacgcc aagaagagaa ggcggcttca gtggccgctg aaacgccttg
     3841 tgagagggga aaacaacccg gagtccctta ttccatgagc tgaagcagaa tctgcgtcta
     3901 acggtgctgg agggccgctg tgtgcacttg tagttatctg tactgaaacc ttggctcgaa
     3961 acgtgaaatg gctaagcagt gcccattcgt tcggtaagaa taataagaag attattattc
     4021 ttattattac caagcaggaa tgagccgtgt gtgttggacg cccataacca gtaacctgcg
     4081 tgaaggtgtc aacatcagga aaggttgccc ctcaagtatc aatacgtcgc cttagtggta
     4141 tgacgggagg ggaggcaaat ttcttgtgtt cattgacact tgaggggcgg gttatgccct
     4201 tcttttttgg cgtccaactc gccccatcgg ggcgaatggt ttgtgtcgat tgttggcatt
     4261 ttaggttccg atttaaagcc gattgagcag cgattttgaa gcgactatgt cgattgcagg
     4321 ccgatttggt gacgattacg gttatgttgc caagaatcgg tgtttgtttg acgataaaac
     4381 gctttacgtt cgatatgcgc tagattttca atagtgggtg aaatgcgggc aggatgggtg
     4441 aagttagcta accggcaagc cggttatcta tcttcactgt cccttattcg cgccgggggc
     4501 actcaacggg aatcctgccc tgcggggctg atcggctacc gccggttgca agagtatagg
     4561 tcttgctatt tttaatatct ttgatagcat ggttgtcggg tacaccaaaa gggtattgat
     4621 atggcaaatg tgcttatcag gaatttaccc gccgaggtgc accgtgcgtt gaaagttcga
     4681 gcagcgctca acgataggag caccgacgcg gaaatacgcg aaatactgac ggcggcagta
     4741 cagccgctag agcttgaaga gattggccgg gaagttggct tgtccgaaag cgataaaaaa
     4801 acagagtagt tttacgtttg taagtccggg cctcagcgcc aagggcaata ccgatcagct
     4861 agtggcgtag ctcttcgatg aaacgttgcc gagaacaagc acaacgcttg gtcaaggcaa
     4921 tgccttcttt tcatcgacgg agtacgacca tgaagtccaa aattttagcg gctaaaaaaa
     4981 ccgctctagc cgttgcactc gcaaccggct ttatcaccac taccaccgcc cctgtgcaag
     5041 ctggtatccc cgtcatcgac ggcggcaacc tggcccagaa catcatgacg gccatcgagt
     5101 cggtggcgca gacgctcaag cagattgagc agtaccagac ccagttgcag cagtacgaaa
     5161 atcagctcca gaacacgatg gccccagccg cgtatatctg ggatcaggcg cagactacga
     5221 tcaaccggct gattgccgcg caaaacacgc tggcctatta cgagaaccag ctaggcagcc
     5281 tggatcgtta cctggccaag tttcaggacg tggcctatta ccgcagctcg ccatgcttca
     5341 acggcagcgg cggatgcacg ccggccgaaa aggcagcgat ggaagagaac cgccgcctgg
     5401 cgtcagagtc gcaaaagaag gccaacgatg ccctgttcca gaccgttgcc gaccagcaaa
     5461 aggctttgaa ggatgacgcc cgtaccctgg agcggctgca aggcgcggcc cagggtgcaa
     5521 ctggccagct acaggccatc ggctacgcca accagctcgc cagccagcag gccaatcagc
     5581 tcttgcagat tcgcaccatg ttgaccgccc aacacaacgc ggaggcagcc cggattgcag
     5641 cagaactcga tgccgaggcg cgaggtgatg ccagggccga gcaaatgcgt acctggacgt
     5701 ttcgcccgag tccggcagac aactactagg gaggcggtga gatgaaaaaa atctttttta
     5761 ctaccgcggc tacgttgctg ttgttggctg gatgtgaaca ggaaaggatg cccgaaccaa
     5821 atgcggcgac gtgtgccccc gatgcgttcc aggcagcact gaatgaaatg cgcagcgaag
     5881 cgaaccgaga ggcctttacc gaggaatgca gagcattcca gaaggccaag cagatgcgtc
     5941 agtgggagtt caagcccagc cctaaagatg attattgagg tggtcgccat gagaaagaaa
     6001 attgctctag gtggcttgat gatggtcgcc acaatggtgc tggctgaacc tgcaatggcg
     6061 caggagctaa gtagcagcgg tgttatgaat gatgtgctca agcgttttca tgatgctgcc
     6121 gcaacatggg ggccagctat tgagtccgct gcatcgcgtt tgttctggac gctggttgtg
     6181 atttcgatgg tctggacatt cggcatgatg gctttgcgca aggccgacat tggcgagttc
     6241 ttcgcggagt tcgttcgctt cacgatcttc accggctttt tctggtggtt gctgacgaat
     6301 gcgaaccagg gcatgaatat cgccggtacg attgttcagt cattgcaaac tctgggcgcg
     6361 caggcggggg gactttccaa tagcaatctt gggccctcca gcatccttga tcttggtttc
     6421 gagttataca accgcacagt acaggccacc tcggagctgg gatggcggca gatggcaact
     6481 gctctggtca tggagctaat ggccctggct gttttgtttg tcctggcgtt gattgcggtc
     6541 aacctgttgc tgttgctcgc atcagcctgg attctgttgt atgccggtgt gttcttcctt
     6601 ggctttggtg gaagtcgttg gacttccgac atggcgatca attactacaa gaccgtgctg
     6661 ggcctggccg cacagcttat ggcaatggtg cttctggttg cgattggcaa agagttcatc
     6721 aatcactact acacgcaaat cagcgagaac atggcatccc aggaactgcc gtgatgttgg
     6781 ttatatcggt catcttgctt ttcttggtca acaaagttcc gcccgatgat ttctggtctt
     6841 gtgtctggtg gtggtattgg cacaaccggt ggaattgaaa ttcggtcggg tcggcagttg
     6901 ggcagtcggt gacggcggcc agtatggcaa ctggcggcgc tgccctggca ggtaaagcgg
     6961 ttatgggtgc cgcagccggt gcagccggag gtgcaagtgc actccaagcg gcttttcaga
     7021 aagcatcagc gagtatggaa accggcggtg acatgtccag catggggtca gttgtcagca
     7081 gtggcggaaa cggtggtggt gaagcgggta ctgctggcag tagcccattc gcccaagcgg
     7141 ctggctttgg tgacagcggc agtagctcaa gcggtggcgg ctttgccaag gccgcgaagc
     7201 tggccacagg cacggcctcc gagttagcca agggtgtcgg ctctcaagtg aagcagggat
     7261 tccaggagcg agtgagcgaa accacaggcg gaaaactggc tgcttcgata cgcgaaagca
     7321 tggagccgaa agaagcaagc caatctggcc agttcgaggg caatagcttg ggcgccgatt
     7381 ctggcccaga tagtaacgaa gtcaggagtt agcacgatga cgacatcaac atacatggcc
     7441 gcattggcgg ccctggatga agcgccaagg gaagaagtca gccaggtatt tatttcgccg
     7501 ctgggtgatc aatcgaccga ggtcgatgca gagacagaaa cagccgcttt tgtatcacgg
     7561 ccagacccta tgagagagag cgaaactaag ccgataagta ttgaggttaa tggcggcagg
     7621 aatgagcggt cagcattcgc ggaggcggcg gggctgtacc tgtaggcgca ccgggctggc
     7681 cacaaccagc ccggcacttt caatcaacac ctactggaga ggtgaattat gacaacacaa
     7741 catattatcg aaccagggca agcagtgcat caagcagcgg ctattctttc ttctttggag
     7801 tacatcaacc aagcggaagc gcggagcctt gggccattgg ccgaagccgt cgctaatgct
     7861 tttatggtgg tgtactacca agctgaaacg ggccgggcga cacaggctga ttttcaagaa
     7921 gcaatgaacg ccttgcgcca agcgtgcagc taatgacgaa agggcggcca ctcgaactgg
     7981 ccgccttttt caatcttcac tgtttggcca ttcccgaccc acagacggcg catccatcca
     8041 ttcgcgttcc tccggcggca gcgggtaagc cccggatgct tccgcgtcgg ccagtagttc
     8101 ggccagtgtg tagcgcaccc gaccagtgga aacggcgcgg gccgggtcgg ccttctcatg
     8161 cagcctgttc tctaggttct ttgggtctgt catcgggcga cctcagcaag catggatact
     8221 aggtgttcca ggtcagtgat cgaatgcgcc aggaagcggc cggcgcggca atctgcaatc
     8281 atttcctcag ttttttccta ctcatgttca acctcgttcg taccagctct tcgagcgatt
     8341 caccacgcgc acgaccagca gcatcaccgg cacttcgacc aggacgccga ccacggttgc
     8401 caatgcagcg ccggagtgca gaccgaacaa gctgatggcg gccgccacgg ccagctcgaa
     8461 gaagttgctg gcaccgatca gggccgaagg acaggcgaca gagtgctttt cccccgcccg
     8521 cttgttcagc cagtaggcta acccggaatt gaagaacact tgaatcaaga tcggcacggc
     8581 tagcatggcg atcactaacg gctgccggat gatggcctcg ccctggaagg cgaacagtag
     8641 aaccaacgtc agcagcagcg cggccatcga ccacggcccg atcttctgca tggcccgttc
     8701 aaaggcggcc tgtccttgct tgagcagatg ccgccgcaag agctgcgcca ggatcaccgg
     8761 cacgatgatg tagagcacga ccgaaatcag cagcgtgtcc caaggcaccg agatcgagga
     8821 catgcccagc agcaggccga cgatgggcgc gaaggccacg atcatgatgg cgtcgttcag
     8881 agccacttgc gagagcgtga aaagcgggtc gccgttggta aggcggctcc acacgaacac
     8941 cattgccgtg cagggcgcgg cggccagcaa gatcaagccc gcgatgtagc tgtccagttg
     9001 atcggccggc aggaacggag cgaacacttg tcggatgaac agccacccca gcagggccat
     9061 cgagaatggc ttgacggccc aattcacgaa cagcgtgacg ccgatgccgc gccaatgctc
     9121 tttgacctgg tgcagcgccc cgaagtcgat cttgaccagc atcgggacga tcattaccca
     9181 aatcagcagg cccaccggca aattgacctg ggccacttcc atgcggccta tggcctggaa
     9241 cacgtcagga aggccctggc cgagcgcaac accggcgacg atgcacaggg ccacccacag
     9301 cgtcaggtaa cgctcgaagc cgctcatcgg ggcctggcgc ggcttcgcgg cgatggcctg
     9361 tgcgccggcg ctcatgcttc ctgctcggtc ttgccgatgt tcgccagctc gcgcttgatg
     9421 gccgtctggt caagcatttt gagaggcaga ttcacgaaca ggcggacgcg gttcatcatg
     9481 tgccggaagg tttgttcgaa ggcccggcgc ttctcggcgt cagtgccttc gacggcggcc
     9541 gggtcttcaa atccccaatg cgccgagatc ggctgaccag gccacaccgg acacatttcg
     9601 ccggcggcgt tgtcgcagac ggtgatgatg aagtccatct tcggtgcgtc gggcgtggcg
     9661 tactcgtccc agctcttgct gcgcagattc tcggtcgggt aattcaccga ctccaccttt
     9721 tcgacggcga agggattgac cttgccggtt gggtgactgc cggcgctgta ggcgcggaag
     9781 cgcccttgcc ccatcgtgtt gatgagagct tcggccatga tgctgcgcgc cgaattgccg
     9841 gtgcagagga tgaggacgtt atagattctt tcggtcatga tgtttcgctt tctggtagtg
     9901 gctggggagc aatggcgatg gatgaaagat cggtggcctc gtcgatgacg tggccggccg
     9961 ctttccgttc ggaatagcgg tctgtcagtg cttcacggtg cggccgtacc agcgccgtga
    10021 agcgcaccag ttcttccatc acatcggcta ttcggtcgta gtacggcgag ggcttcatgc
    10081 ggcccgccgc gtcgaactct tggaacgctt tggcgatact cgactggttc ggaatggtga
    10141 acattcgcat ccagcggccg agcagacgca aggtgttcac ggcgttgaag ctctgcgagc
    10201 cgccggatac ctgcatcacg gccagggtgc ggccttgggt cggccggatg ccggccattt
    10261 caagcggcag atggtcaatc tgcgccttca tgacactggt aatctgaccg tggcgttccg
    10321 ggctgcacca gacttgtccc tctgaccact cggacagggc gcgcagctcc ttgacggccg
    10381 ggtgatcgtc gctttgcact tgatcgggca acggcaaatc ggacgggtcg aagatgcgtg
    10441 tttctgcgcc aaagaattgc agcagccggg ccgcttcctc gacggccagc cgtgagaacg
    10501 agcgggcgcg cagcgagcca tacagcagca ggatacgcac cggcggggcg tcgggtgcca
    10561 gcccgagcgc cgggcgctcg atggcaaagg atttgtccaa agcgggcaaa gaatcggggt
    10621 cggaaagatg gcgaagtctc atgccaacac cttcccggct tccgtggtgc aagacaggtt
    10681 gcacacctgg ccgccgcagc agttttccgt gagaaaggcc accaggttag tggccgtcga
    10741 aaagttcgcc tcgtagatca caaatcggcc ttcttgccgc gacgtgacca tgccggcgtg
    10801 tgccagctct ttcaggtgga aggatagcga agacggcggg atgccggccg cttcgctgat
    10861 ttttccggcg gccatgccgg cggggccggc ctggacgaga agccggaaca ccgcgaggcg
    10921 cgattcttga gcgatggccg ccagggcggc tacagcattt atcgtttcca tgtttcaata
    10981 atagtcgaaa tatggagtgt gctcaatagc tcatttaagc tgtaaaaatg agctaggcgc
    11041 atttagatcg atatgtgata aattggcctg ccagtttaag atgggtgcat ttgagtatgc
    11101 ccaaaggagc ccgcaagtat gcgcaggacg aagccagtag ccgcgccgat ggtggcgcgg
    11161 gtctatctgc gcgtcagcac cgacgcgcag gacttggaac gccaagaggc gatcactacg
    11221 gccgcgaagg ccgccggcta ctacgtcgcc ggcatctacc gtgagaaggc atccggcgca
    11281 cgcgccgacc ggcctgagct gctgcgcatg atcggcgacc tacagcccgg cgaggtggtc
    11341 attgccgaga agatcgaccg catcagccgc ctacctttgc ccgaggccga gcgcctggtg
    11401 gcctcgatac aggccaaagg cgcacgcctg gccgtccctg gcgtggtcga tctatccgac
    11461 ctggcggccg aggcccaggg cgtcgccaag atcgtgctgg aagccgtgca gatcatgctt
    11521 tttcgcctgg ccttgcagat ggcccgcgac gactacgagg acaggcgcga acgccagcgc
    11581 caaggcattg agttggcccg ccaggccggg cggtacaagg gccgccgtgc tgatccgaag
    11641 cgccgcgccc aagttgtcgc gctgcgcaag tccggctaca gcatcaacaa gaccgccgag
    11701 ctggccgggt acagtgcggc ccaggtgaaa cggatatggg ccgaggtcag ccaggccgaa
    11761 gcgaagcagc acggcgcgtt cgtggaggac gcattgacgg aagccgatgc cctggccgct
    11821 gtcggccagg atgagcgcca ggaggaaagg gcatgaagaa gccgaaccaa gacgacgagc
    11881 cgtttttcat caccgaggag attgcggccg aaatgatcgc cggcggctat gagttcgagc
    11941 tgccgcccat tccttgcacc atccgcctac gcgacgtgct ggagcgcatg accgatgctg
    12001 agctagcatt gcagccgggc gagatcgccg accaggagcg tgaacgctgc cggcgcaagc
    12061 cgtgttcaac ctcatgatct ggtcatggta tttttcatgg cactgagcct gatagttctt
    12121 gcaaattgtt gtcactaaag ggttttgtgt gcttgtttac aatcgagtgg gagtgacggg
    12181 cactggctgg caatgtctag caacggcagg catttcggct gagggtaaaa gaactttccg
    12241 ctaagcgata gactgtatgt aaacacagta ttgcaaggac gcggaacatg cctcatgtgg
    12301 cggccaggac ggccagccgg gatcgggata ctggtcgtta ccagagccac cgacccgagc
    12361 aaacccttct ctatcagatc gttgacgagt attacccggc attcgctgcg cttatggcag
    12421 agcagggaaa ggaattgccg ggctatgtgc aacgggaatt tgaagaattt ctccaatgcg
    12481 ggcggctgga gcatggcttt ctacgggttc gctgcgagtc ttgccacgcc gagcacctgg
    12541 tcgctttcag ctgtaagcgt cgcggtttct gcccgagctg tggggcgcgg cggatggccg
    12601 aaagtgccgc cttgctggtt gatgaagtac tgcctgaaca acccatgcgt cagtgggtgt
    12661 tgagcttccc gtttcagctg cgtttcctgt ttgccagccg gcccgagatc atggggtggg
    12721 tgctgggcat cgtttaccgc gtcattgcca cgcacctggt caagaaagcg ggccataccc
    12781 accaagtggc caagacgggc gcggtcaccc tgatccagcg ttttggatcg gcgctcaatc
    12841 tgaatgttca cttccacatg ctgtttctcg acggtgtgta tgtcgagcaa tcccacggct
    12901 cagcgcgttt ccgctgggtc aaggcgccga ccagcccaga gctcacccag ctgacgcaca
    12961 ccatcgccca ccgggtgggt cgctatctgg aacggcaagg cctgctggaa cgggatgtcg
    13021 aaaacagcta tctggcctcg gatgcggtgg atgacgaccc gatgacaccc ctgctggggc
    13081 actcgatcac ttaccgtatc gctgtcggtt cacaggcggg gcgaaaggtg ttcactttgc
    13141 aaactctgcc gaccagtggt gatccgttcg gtgacgggat tggcaaggta gccgggtcca
    13201 gcctgcacgc cggcgtggcg gccagggccg atgaacgcaa gaagctcgaa cggctgtgcc
    13261 ggtacatcag ccgcccggcg gtatccgaga agcggctgtc gttaacacga ggcggcaacg
    13321 tgcgctacca gctcaagacg ccgtaccggg acggcaccac gcacgtcatt ttcgaaccat
    13381 tggatttcat tgcaaggctg gccgccctgg taccgaagcc cagagtcaac ctaacccgct
    13441 tccacggggt gttcgcaccc aacagtcggc accgggcgtt ggtcacgccg gcaaaacggg
    13501 gcaggggcaa caaggtcagg gtggctgatg aaccggcaac accagcacaa cggcgagcgt
    13561 cgatgacatg ggcgcaacgg ctcaagcgtg ttttcaatat cgacatcgag acctgcagcg
    13621 gctgcggcgg cgccatgaaa gtcatcgcct gcattgaaga ccctatagtg atcaagcaga
    13681 tccttgatca cctgaagcac aaagccgaaa ccagcgggac cagggcgtta cccgaaagcc
    13741 gggcgccacc ggctgagctg ctcctgggtc tgtttgactg acgagcctga aggccaacga
    13801 taccaatcaa aatgctgcgt tcacagcgcc gcggcaggga tccgccgtgc tggttgtcgg
    13861 aaaaggagcc gctagtggga aagaggaggg taaattttca gcgttgctgg ctccccgtca
    13921 gccggattgg gttgcatcgc aggggtgtcg aaagagtcaa ctgcggtcca aagctgttgg
    13981 acttgggtga aaagggcgtt tattcttcct atacgttgtc ggcagcgggc caaaaaggaa
    14041 tacgtccatg cccatcgagg tgaaaccggc tgtgagcgcg ggttcaagca tatagcccga
    14101 caggcgcgta tccttgccga tcacgacacg atggcggtgg tcaccgcgac gaaagacacg
    14161 gccagccgcc atgccgacgc gcaaggcggt ttccgccgtc atcgcgcctt cgttggcttt
    14221 gccacgaata ccgtctgtgc cgaaatattt gcgcaccata aggtcgatta tcctgtcgtc
    14281 gggtcgccct caaaggggac atgcctgctg aaccgcgaat atagagaaat atcccgaatg
    14341 tgcagttaac gaattcttgc ggtttctttc agcgccgcca ataccgccag cccgtcgcgc
    14401 aaggggcgcg gctcgtgtgt gcggatgaag tcagctccac ctgcggcggc ggcaagctct
    14461 gcagcgagtg tcgcggcccc gacatccccc ggaccacggc ctgtgagcgc gcgcagaaag
    14521 gatttgcgcg aaacagacag aagcaccggc aaatcgaagc gcagccgcaa ttcatcgaac
    14581 cgcgccagca ccgagagcga ggtttcggga gcagccccca gaaaaaaccc catgccggga
    14641 tcaaggacaa ggcggttgcg tttgataccg gcacccgtca gcgccgcgat gcgcgcgtca
    14701 aagaacgccg caatgtgatc catgatgtcg ccagcgggtg cctcgcgccg atctgcctgc
    14761 ccgtcttgca ccgaatgcat aacgacgagt ttggcagatg atttcgccaa ttgcggatag
    14821 aacgcagcgt ctggaaaacc gcgaatatca ttgagatagg ccacaccacg cgacaaggca
    14881 taggcttgcg tcgcgggttg ataactgtcg agcgagacgg gaatgccatc tgccttgagc
    14941 gcgtccagca ccggcgcgat acgcgcgatt tctgtgtcgg acgaaacagg cgcggcgtcg
    15001 ggattgctgg atgccggacc gaggtcgatc acatctgccc cctcggccat cagcttacgc
    15061 gcctgcgcaa tggctgcgtc tggcgccaga taccggcctc catcggagaa actgtccgag
    15121 gttatgttga cgatgccgaa aatgatgagc gatttattca tgggggcttc tataataata
    15181 ataatcgagc atgagtctca tacggatgct cgggtcgaaa gggaatcccc aggcgagtaa
    15241 cctgtttgcg gtgatccatt agctgcagga gcagaatagc atacatctgg aagcaaagcc
    15301 aggaaagcgg cctatggagc tgtgcggcag cgctcagtag gcaatttttc aaaatattgt
    15361 taagcctttt ctgagcatgg tatttttcat ggtattacca attagcagga aaataagcca
    15421 ttgaatataa aagataaaaa tgtcttgttt acaatagagt ggga
//
