LOCUS       Tn6590                 15621 bp    DNA     linear       26-OCT-2020
DEFINITION  Providencia sp.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Providencia sp.
  ORGANISM  Providencia sp.
            Unclassified.
REFERENCE   1  (bases 1 to 15621)
  AUTHORS   Zhou,D.
  TITLE     The complete sequence of Tn6590
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15621)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: HGAP v. v3.0
            Sequencing Technology :: PacBio
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15621
                     /organism="Providencia sp."
                     /mol_type="genomic DNA"
     mobile_element  1..15621
                     /gene="Tn6590"
                     /product="Integrative and mobilizable element:  Tn6590"
     repeat_region   1..8
                     /gene="attL _Tn6590"
                     /product="'Attachment site at the left end of Tn6590"
     CDS             317..1519
                     /gene="int"
                     /codon_start=1
                     /transl_table=11
                     /product="Site-specific integrase"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVAVTPAGSISFRYNYSIN
                     GRQETITFGRYGVGGITLAEARELLGDAKKMVAAGKSPAKEKARDKARVKDAETFGAW
                     AEKWLRGYQMADSTRDMRRSVYERELKPKFSNQKLVEITHEDLRALADAIVERGAPAT
                     AVHVREIVLQVFRWAIERGQKVENPAELVRPTSIARFEPRDRALTPEEIGLMYQYMER
                     VGTSPTNRAAAKLLLLTMVRKSELTNATWSEINFSEALWTIPKERMKRRNPHLVFLSQ
                     QALDIFIAMKTFAGGSDFVLPSRYDSDAPMSAATLNQVLTLTYKAAQKDGKSLTKFGP
                     HDLRRTASTLLHEAGYNTDWIEKCLAHEQKGVRAVYNKAEYREQRAAMLQDWADMIDE
                     WTSGGSKG"
     CDS             1667..1888
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /translation="MQNIKTLINRKKLLEMIPLSTRTIYNLEQRGDFPRRIALTSRNV
                     AWDLSEVEEWIEARKSSGDQAARPGPIEG"
     CDS             1893..2720
                     /gene="repA"
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein RepA"
                     /translation="MALDLMAAFTELPPPIDYVLPNMVAGTVGALVSPGGAGKSMLAL
                     QLAAQIAGGPDLLEIGEFPTGQVVYLPAEDPPAAIHHRLHALGAHLSAAERQAVADGL
                     LIEPLIGKCPNIMAASWFDALKRAAEGRRLMILDTLRRFHIEEENASGPMAQVVGHME
                     AIAADTGCSIVFLHHASKSAAMMGSGDQQQASRGSSVLVDNIRWQSYLSGMTQGEAEI
                     LGVDDCQRGYFVRFGVSKANYGAPFQELWFRRHDGGVLKPAVLERQCKVKRRQREEA"
     CDS             2707..3588
                     /gene="repC"
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein RepC"
                     /translation="MKKPKHDLTHVRHDPAHCLAPGLFRSLKRGDRKRCKLDVTYTFG
                     EDESMRFVGFEPLGADDMRLLQGIVALGGPNGILLTPEPTSETGRQLRLFLEPRFEAI
                     EQDGLVVRESLTKLLSETGMTDSGDNIKALKASLLRMSNVTILVTKGRRQAAFHLMSH
                     AFDETDGRLWVALNPRIAEAILGHRPYARIDMAEVRVLQTDPARLMHQRLCGWIDPGK
                     SGRVELDTLCGYVWPDEANAEAMKKRRQTARKALAELAAVGWVVNEYAKGKWEIKRPG
                     PTATAPVYRRNVPLLPS"
     CDS             complement(3818..4033)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MVIIRIIIFLLFLPNEWALLSHFTFRAKVSVQITTSAHSGPPAP
                     LDADSASAHGIRDSGLFSPLTRRFSGH"
     misc_feature    4282..4553
                     /gene="oriT"
                     /product="Origin region of transfer"
                     /note="UNKNOWN FEATURE KEY misc_rescomb"
     CDS             4621..4809
                     /gene="stbC"
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid stability protein StbC"
                     /translation="MANVLIRNLPAEVHRALKVRAALNDRSTDAEIREILTAAVQPLE
                     LEEIGREVGLSESDKKTE"
     CDS             4950..5729
                     /gene="tivB8"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type type IV secretion, bitopic protein/inner
                     membrane complex component"
                     /translation="MKSKILAAKKTALAVALATGFITTTTAPVQAGIPVIDGGNLAQN
                     IMTAIESVAQTLKQIEQYQTQLQQYENQLQNTMAPAAYIWDQAQTTINRLIAAQNTLA
                     YYENQLGSLDRYLAKFQDVAYYRSSPCFNGSGGCTPAEKAAMEENRRLASESQKKAND
                     ALFQTVADQQKALKDDARTLERLQGAAQGATGQLQAIGYANQLASQQANQLLQIRTML
                     TAQHNAEAARIAAELDAEARGDARAEQMRTWTFRPSPADNY"
     CDS             5742..5978
                     /gene="eex"
                     /codon_start=1
                     /transl_table=11
                     /product="Entry exclusion protein"
                     /translation="MKKIFFTTAATLLLLAGCEQERMPEPNAATCAPDAFQAALNEMR
                     SEANREAFTEECRAFQKAKQMRQWEFKPSPKDDY"
     CDS             5989..7416
                     /gene="tivB6"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type type IV secretion, polytopic
                     protein/inner membrane complex component"
                     /translation="MRKKIALGGLMMVATMVLAEPAMAQELSSSGVMNDVLKRFHDAA
                     ATWGPAIESAASRLFWTLVVISMVWTFGMMALRKADIGEFFAEFVRFTIFTGFFWWLL
                     TNANQGMNIAGTIVQSLQTLGAQAGGLSNSNLGPSSILDLGFELYNRTVQATSELGWR
                     QMATALVMELMALAVLFVLALIAVNLLLLLASAWILLYAGVFFLGFGGSRWTSDMAIN
                     YYKTVLGLAAQLMAMVLLVAIGKEFINHYYTQISENMASQELAVMLVISVILLFLVNK
                     VPPMISGLVSGGGIGAAGGIGNFGAGAAVGAAVTAASMATGGAALAGKAVMGAAAGAA
                     GGASALQAAFQKASASMETGGDMSSMGSVVSSGGNGGGEAGTAGSSPFAQAAGFGDSG
                     SSSSGGGFAKAAKLATGTASELAKGVGSQVKQGFQERVSETTGGKLAASIRESMEPKE
                     ASQSGQFEGNSLGADSGPDSNEVRS"
     CDS             7421..7669
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MTTSTYMAALAALDEAPREEVSQVFISPLGDQSTEVDAETETAA
                     FVSRPDPMRESETKPISIEVNGGRNERSAFAEAAGLYL"
     CDS             7733..7957
                     /codon_start=1
                     /transl_table=11
                     /product="Type I toxin-antitoxin system ptaRNA1 family
                     toxin"
                     /translation="MTTQHIIEPGQAVHQAAAILSSLEYINQAEARSLGPLAEAVANA
                     FMVVYYQAETGRATQADFQEAMNALRQACS"
     CDS             complement(7994..8197)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MTDPKNLENRLHEKADPARAVSTGRVRYTLAELLADAEASGAYP
                     LPPEEREWMDAPSVGREWPNSED"
     CDS             complement(8312..9379)
                     /gene="acr3"
                     /codon_start=1
                     /transl_table=11
                     /product="Arsenical-resistance protein ACR3"
                     /translation="MSAGAQAIAAKPRQAPMSGFERYLTLWVALCIVAGVALGQGLPD
                     VFQAIGRMEVAQVNLPVGLLIWVMIVPMLVKIDFGALHQVKEHWRGIGVTLFVNWAVK
                     PFSMALLGWLFIRQVFAPFLPADQLDSYIAGLILLAAAPCTAMVFVWSRLTNGDPLFT
                     LSQVALNDAIMIVAFAPIVGLLLGMSSISVPWDTLLISVVLYIIVPVILAQLLRRHLL
                     KQGQAAFERAMQKIGPWSMAALLLTLVLLFAFQGEAIIRQPLVIAMLAVPILIQVFFN
                     SGLAYWLNKRAGEKHSVACPSALIGASNFFELAVAAAISLFGLHSGAALATVVGVLVE
                     VPVMLLVVRVVNRSKSWYERG"
     CDS             complement(9376..9882)
                     /gene="arsC"
                     /codon_start=1
                     /transl_table=11
                     /product="Arsenate reductase ArsC"
                     /translation="MTERIYNVLILCTGNSARSIMAEALINTMGQGRFRAYSAGSHPT
                     GKVNPFAVEKVESVNYPTENLRSKSWDEYATPDAPKMDFIITVCDNAAGEMCPVWPGQ
                     PISAHWGFEDPAAVEGTDAEKRRAFEQTFRHMMNRVRLFVNLPLKMLDQTAIKRELAN
                     IGKTEQEA"
     CDS             complement(9879..10646)
                     /gene="arsH"
                     /codon_start=1
                     /transl_table=11
                     /product="Arsenical resistance protein ArsH"
                     /translation="MRLRHLSDPDSLPALDKSFAIERPALGLAPDAPPVRILLLYGSL
                     RARSFSRLAVEEAARLLQFFGAETRIFDPSDLPLPDQVQSDDHPAVKELRALSEWSEG
                     QVWCSPERHGQITSVMKAQIDHLPLEMAGIRPTQGRTLAVMQVSGGSQSFNAVNTLRL
                     LGRWMRMFTIPNQSSIAKAFQEFDAAGRMKPSPYYDRIADVMEELVRFTALVRPHREA
                     LTDRYSERKAAGHVIDEATDLSSIAIAPQPLPESETS"
     CDS             complement(10643..10975)
                     /gene="arsR"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator, ArsR
                     family"
                     /translation="METINAVAALAAIAQESRLAVFRLLVQAGPAGMAAGKISEAAGI
                     PPSSLSFHLKELAHAGMVTSRQEGRFVIYEANFSTATNLVAFLTENCCGGQVCNLSCT
                     TEAGKVLA"
     CDS             11123..11860
                     /gene="resG"
                     /codon_start=1
                     /transl_table=11
                     /product="Resolvase"
                     /translation="MRRTKPVAAPMVARVYLRVSTDAQDLERQEAITTAAKAAGYYVA
                     GIYREKASGARADRPELLRMIGDLQPGEVVIAEKIDRISRLPLPEAERLVASIQAKGA
                     RLAVPGVVDLSDLAAEAQGVAKIVLEAVQIMLFRLALQMARDDYEDRRERQRQGIELA
                     RQAGRYKGRRADPKRRAQVVALRKSGYSINKTAELAGYSAAQVKRIWAEVSQAEAKQH
                     GAFVEDALTEADALAAVGQDERQEERA"
     CDS             11857..12081
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MKKPNQDDEPFFITEEIAAEMIAGGYEFELPPIPCTIRLRDVLE
                     RMTDAELALQPGEIADQERERCRRKPCSTS"
     mobile_element  complement(12175..12697)
                     /gene="truncated ISCR2"
                     /product="Truncated Insertion sequence: ISCR2"
     repeat_region   12175..12190
                     /gene="terIS"
                     /product="terIS of ISCR2"
     misc_feature    12292..12831
                     /gene="truncated tnpA"
                     /product="Truncated ISCR2 Transposase"
     mobile_element  complement(12697..14474)
                     /gene="truncated Tn5393c"
                     /product="Truncated unit transposon: Tn5393c"
     repeat_region   complement(12697..12777)
                     /gene="IRR_Tn5393c"
                     /product="Tn5393c inverted repeat right"
     CDS             complement(12803..13639)
                     /gene="strB"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 6-phosphotransferase"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     CDS             complement(13639..14442)
                     /gene="strA"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3''-phosphotransferase"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     CDS             complement(14503..15318)
                     /gene="sul2"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydropteroate synthase type-2"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     repeat_region   15614..15621
                     /gene="attR_Tn6590"
                     /product="'Attachment site at the right end of Tn6590"
BASE COUNT     3479 a   4208 c   4699 g   3235 t
ORIGIN
        1 gagtgggaat gattccgcgt ctggcacagc caggcacctg atggcatgaa atgccgctaa
       61 gcccgcgtaa ttgcgggctt ttttttgttt gtgtttggca cgatctggca acgggaggaa
      121 gcgccaagca ccgtttgagc atggcattaa agctggtatt atggtttggc gatgccatga
      181 ttgatgccga taccatgctg cgttcttttg tgtgttcatg gtattaatat tctataagtt
      241 actgtttcgt aagaaaaaat acgataaatt cgagattttt tcagcatggt atcggagacg
      301 aagaaggaca aggtacatgc tgaccgatac caagctgcgc aatctcaagc ccagggacaa
      361 actctacaaa gtgaatgacc gggaaggtct ctatgtggca gtgactccag ccggctccat
      421 ctcgttccgt tacaactact caatcaacgg tcggcaggag accatcacct ttgggcgtta
      481 tggtgtcggt ggcatcaccc tggccgaagc ccgcgagctg ttgggtgacg ccaagaagat
      541 ggttgcggcg ggcaagtcgc cggccaagga gaaagcccga gacaaggcgc gggtgaaaga
      601 tgcagagacg ttcggtgcct gggcggagaa gtggctgcgt ggctaccaga tggccgactc
      661 cacccgcgat atgcgccgct cggtttatga gcgtgagctg aagccgaaat tcagcaatca
      721 gaagctggtg gagatcaccc acgaagactt gcgggcgctg gctgatgcca ttgttgagcg
      781 aggcgcaccg gccaccgccg ttcatgtgcg tgaaatcgtg ttgcaggtat ttcgctgggc
      841 catcgagcgt gggcagaagg tcgaaaaccc ggcagaactg gtgcgcccaa caagcatcgc
      901 tcgattcgag ccacgtgacc gagcgttgac gccagaagaa attgggctga tgtaccagta
      961 catggagcga gtgggcacaa gcccaacaaa ccgtgcggcg gccaagctgt tattgctgac
     1021 gatggtgcgc aagagcgagc tgaccaatgc gacctggagc gagatcaatt tcagcgaagc
     1081 gttgtggacg attccaaagg agcggatgaa acgccgtaac ccgcacctgg tatttctgtc
     1141 ccagcaggct ctggatattt tcattgccat gaaaaccttt gccggtggtt ccgacttcgt
     1201 tttgccatca cggtacgact cggatgcgcc gatgagcgct gccacactta accaggtgct
     1261 gacgctgact tacaaggcgg cgcagaaaga tgggaagtca cttaccaagt tcggaccgca
     1321 tgatttgcgg cgcacagcca gcacgctgtt gcatgaggcc ggctacaaca ccgactggat
     1381 cgagaagtgc ctggcgcacg agcagaaggg cgtgagggct gtctacaaca aggctgagta
     1441 ccgcgagcag cgggcggcga tgttgcagga ttgggccgat atgattgatg agtggacttc
     1501 gggaggcagt aagggttgat gtttttgcta tctttgatag cattgaagtt gatgccgtga
     1561 atttataaat aaggccgcca ttggcatcgg gcaggtcaat taccgattag ctgcttcgcg
     1621 cagctactcg atgaatagca actattcatt tgagaggtat tgacccatgc aaaatataaa
     1681 gaccctcatc aacaggaaga agctcctgga gatgatcccg ctttcgacac gaacaattta
     1741 taacctggag cagcgagggg attttccacg ccgtatcgcg ttaaccagta gaaatgtcgc
     1801 ctgggatttg tcagaggtcg aagagtggat tgaggcacgt aaatcatcgg gtgatcaagc
     1861 tgcgcgacct ggccctatag agggctagtt atatggctct tgatcttatg gcggctttca
     1921 cggaattgcc gccacccatt gattatgttc tgcctaatat ggttgctggc actgttggtg
     1981 ctcttgtgtc gcctggtggg gctggtaaat ccatgttggc ccttcaattg gctgcacaga
     2041 ttgcaggcgg gcctgattta cttgaaatag gcgagtttcc caccgggcaa gtggtctatc
     2101 tgcctgctga agatccaccg gccgctattc accatcgtct gcacgccctt ggggcacacc
     2161 tcagcgcagc ggaacggcaa gccgtggctg atggtttgct cattgaaccc ttgatcggta
     2221 aatgcccaaa catcatggct gctagctggt tcgatgctct caaacgagcc gctgaaggtc
     2281 gtcgcttgat gatcttggac actttgcggc gcttccacat tgaggaggag aacgctagcg
     2341 ggccgatggc gcaggttgtt gggcacatgg aggccatagc cgctgataca ggctgctcca
     2401 ttgtgtttct gcaccacgcg agtaaaagcg cagccatgat ggggtcgggc gatcaacagc
     2461 aggccagtcg tggatcgtcc gtactggttg ataacatccg ttggcaatcg tacctatccg
     2521 gcatgacgca aggcgaggcc gagatactgg gggtcgatga ttgtcagcgt gggtattttg
     2581 tccgctttgg cgtcagcaag gccaactatg gcgcaccttt tcaagaactc tggtttagac
     2641 ggcacgacgg cggcgtgttg aagcctgctg tactggagcg gcagtgcaag gtgaaaagga
     2701 gacagcgtga agaagcctaa gcatgacctg acccacgtcc gacatgatcc cgcgcactgt
     2761 ttggcacctg gcctgttccg cagcctcaag cgtggcgatc gcaaacgctg caagctggac
     2821 gtgacctaca cctttggcga ggacgaatcc atgcgtttcg tcggattcga acctctcggg
     2881 gccgatgata tgcgtctttt gcaaggcatc gtggcccttg gcggcccgaa cggcatcttg
     2941 ctaaccccgg aaccgaccag tgagacgggg cgacagctac ggctattcct tgaaccccgt
     3001 ttcgaagcca ttgagcaaga cggcttggtg gttcgtgaga gcctgaccaa actgctctca
     3061 gaaacgggca tgacggatag cggcgacaac atcaaggcgc tcaaagccag cctgctgcgc
     3121 atgtcgaacg tcaccatcct tgtgacgaag ggacggcggc aagccgcgtt ccacctgatg
     3181 agtcatgctt ttgacgagac ggacggcagg ctatgggttg ccctgaatcc gcgtattgcc
     3241 gaagcgatcc tggggcatcg tccatatgcc cgtatcgaca tggcggaagt gcgggtgcta
     3301 cagactgatc cggcacggct gatgcaccaa cggctatgcg gctggatcga ccccggcaaa
     3361 tccgggcgcg tggaactgga cacgctttgc ggctatgtct ggccagatga agccaatgcc
     3421 gaagctatga aaaaacgccg tcagactgcc cggaaggcac tggccgaact tgccgccgtg
     3481 ggttgggtag tgaacgaata cgccaaggga aaatgggaga tcaagaggcc tggccccacg
     3541 gcaactgcac ccgtttaccg tcgtaacgtt cccttgttac cgtcgtaacg ctccccttct
     3601 gccgtcgtaa cgttccccgt cccaatttgg aaaacccaga cggtgcgctg ctttgcgggc
     3661 agtttgggaa atccatccaa gattatctaa agataatcca tcatgcgcgg tgcagctcgc
     3721 gccttgaggg cgcgttctga ccttgccaat aaagccctgg cgggctttat caatcggcca
     3781 taggccgatg gtttaacgcc aagaagagaa ggcggcttca gtggccgctg aaacgccttg
     3841 tgagagggga aaacaacccg gagtccctta ttccatgagc tgaagcagaa tctgcgtcta
     3901 acggtgctgg agggccgctg tgtgcacttg tagttatctg tactgaaacc ttggctcgaa
     3961 acgtgaaatg gctaagcagt gcccattcgt tcggtaagaa taataagaag attattattc
     4021 ttattattac caagcaggaa tgagccgtgt gtgttggacg cccataacca gtaacctgcg
     4081 tgaaggtgtc aacatcagga aaggttgccc ctcaagtatc aatacgtcgc cttagtggta
     4141 tgacgggagg ggaggcaaat ttcttgtgtt cattgacact tgaggggcgg gttatgccct
     4201 tcttttttgg cgtccaactc gccccatcgg ggcgaatggt ttgtgtcgat tgttggcatt
     4261 ttaggttccg atttaaagcc gattgagcag cgattttgaa gcgactatgt cgattgcagg
     4321 ccgatttggt gacgattacg gttatgttgc caagaatcgg tgtttgtttg acgataaaac
     4381 gctttacgtt cgatatgcgc tagattttca atagtgggtg aaatgcgggc aggatgggtg
     4441 aagttagcta accggcaagc cggttatcta tcttcactgt cccttattcg cgccgggggc
     4501 actcaacggg aatcctgccc tgcggggctg atcggctacc gccggttgca agagtatagg
     4561 tcttgctatt tttaatatct ttgatagcat ggttgtcggg tacaccaaaa gggtattgat
     4621 atggcaaatg tgcttatcag gaatttaccc gccgaggtgc accgtgcgtt gaaagttcga
     4681 gcagcgctca acgataggag caccgacgcg gaaatacgcg aaatactgac ggcggcagta
     4741 cagccgctag agcttgaaga gattggccgg gaagttggct tgtccgaaag cgataaaaaa
     4801 acagagtagt tttacgtttg taagtccggg cctcagcgcc aagggcaata ccgatcagct
     4861 agtggcgtag ctcttcgatg aaacgttgcc gagaacaagc acaacgcttg gtcaaggcaa
     4921 tgccttcttt tcatcgacgg agtacgacca tgaagtccaa aattttagcg gctaaaaaaa
     4981 ccgctctagc cgttgcactc gcaaccggct ttatcaccac taccaccgcc cctgtgcaag
     5041 ctggtatccc cgtcatcgac ggcggcaacc tggcccagaa catcatgacg gccatcgagt
     5101 cggtggcgca gacgctcaag cagattgagc agtaccagac ccagttgcag cagtacgaaa
     5161 atcagctcca gaacacgatg gccccagccg cgtatatctg ggatcaggcg cagactacga
     5221 tcaaccggct gattgccgcg caaaacacgc tggcctatta cgagaaccag ctaggcagcc
     5281 tggatcgtta cctggccaag tttcaggacg tggcctatta ccgcagctcg ccatgcttca
     5341 acggcagcgg cggatgcacg ccggccgaaa aggcagcgat ggaagagaac cgccgcctgg
     5401 cgtcagagtc gcaaaagaag gccaacgatg ccctgttcca gaccgttgcc gaccagcaaa
     5461 aggctttgaa ggatgacgcc cgtaccctgg agcggctgca aggcgcggcc cagggtgcaa
     5521 ctggccagct acaggccatc ggctacgcca accagctcgc cagccagcag gccaatcagc
     5581 tcttgcagat tcgcaccatg ttgaccgccc aacacaacgc ggaggcagcc cggattgcag
     5641 cagaactcga tgccgaggcg cgaggtgatg ccagggccga gcaaatgcgt acctggacgt
     5701 ttcgcccgag tccggcagac aactactagg gaggcggtga gatgaaaaaa atctttttta
     5761 ctaccgcggc tacgttgctg ttgttggctg gatgtgaaca ggaaaggatg cccgaaccaa
     5821 atgcggcgac gtgtgccccc gatgcgttcc aggcagcact gaatgaaatg cgcagcgaag
     5881 cgaaccgaga ggcctttacc gaggaatgca gagcattcca gaaggccaag cagatgcgtc
     5941 agtgggagtt caagcccagc cctaaagatg attattgagg tggtcgccat gagaaagaaa
     6001 attgctctag gtggcttgat gatggtcgcc acaatggtgc tggctgaacc tgcaatggcg
     6061 caggagctaa gtagcagcgg tgttatgaat gatgtgctca agcgttttca tgatgctgcc
     6121 gcaacatggg ggccagctat tgagtccgct gcatcgcgtt tgttctggac gctggttgtg
     6181 atttcgatgg tctggacatt cggcatgatg gctttgcgca aggccgacat tggcgagttc
     6241 ttcgcggagt tcgttcgctt cacgatcttc accggctttt tctggtggtt gctgacgaat
     6301 gcgaaccagg gcatgaatat cgccggtacg attgttcagt cattgcaaac tctgggcgcg
     6361 caggcggggg gactttccaa tagcaatctt gggccctcca gcatccttga tcttggtttc
     6421 gagttataca accgcacagt acaggccacc tcggagctgg gatggcggca gatggcaact
     6481 gctctggtca tggagctaat ggccctggct gttttgtttg tcctggcgtt gattgcggtc
     6541 aacctgttgc tgttgctcgc atcagcctgg attctgttgt atgccggtgt gttcttcctt
     6601 ggctttggtg gaagtcgttg gacttccgac atggcgatca attactacaa gaccgtgctg
     6661 ggcctggccg cacagcttat ggcaatggtg cttctggttg cgattggcaa agagttcatc
     6721 aatcactact acacgcaaat cagcgagaac atggcatccc aggaactggc cgtgatgttg
     6781 gttatatcgg tcatcttgct tttcttggtc aacaaagttc cgccgatgat ttctggtctt
     6841 gtgtctggtg gtggtattgg cgcagccggt ggaattggaa acttcggtgc gggtgcggca
     6901 gttggggcgg cggtgacggc ggccagtatg gcaactggcg gcgctgccct ggcaggtaaa
     6961 gcggttatgg gtgccgcagc cggtgcagcc ggaggtgcaa gtgcactcca agcggctttt
     7021 cagaaagcat cagcgagtat ggaaaccggc ggtgacatgt ccagcatggg gtcagttgtc
     7081 agcagtggcg gaaacggtgg tggtgaagcg ggtactgctg gcagtagccc attcgcccaa
     7141 gcggctggct ttggtgacag cggcagtagc tcaagcggtg gcggctttgc caaggccgcg
     7201 aagctggcca caggcacggc ctccgagtta gccaagggtg tcggctctca agtgaagcag
     7261 ggattccagg agcgagtgag cgaaaccaca ggcggaaaac tggctgcttc gatacgcgaa
     7321 agcatggagc cgaaagaagc aagccaatct ggccagttcg agggcaatag cttgggcgcc
     7381 gattctggcc cagatagtaa cgaagtcagg agttagcacg atgacgacat caacatacat
     7441 ggccgcattg gcggccctgg atgaagcgcc aagggaagaa gtcagccagg tatttatttc
     7501 gccgctgggt gatcaatcga ccgaggtcga tgcagagaca gaaacagccg cttttgtatc
     7561 acggccagac cctatgagag agagcgaaac taagccgata agtattgagg ttaatggcgg
     7621 caggaatgag cggtcagcat tcgcggaggc ggcggggctg tacctgtagg cgcaccgggc
     7681 tggccacaac cagcccggca ctttcaatca acacctactg gagaggtgaa ttatgacaac
     7741 acaacatatt atcgaaccag ggcaagcagt gcatcaagca gcggctattc tttcttcttt
     7801 ggagtacatc aaccaagcgg aagcgcggag ccttgggcca ttggccgaag ccgtcgctaa
     7861 tgcttttatg gtggtgtact accaagctga aacgggccgg gcgacacagg ctgattttca
     7921 agaagcaatg aacgccttgc gccaagcgtg cagctaatga cgaaagggcg gccactcgaa
     7981 ctggccgcct ttttcaatct tcactgtttg gccattcccg acccacagac ggcgcatcca
     8041 tccattcgcg ttcctccggc ggcagcgggt aagccccgga tgcttccgcg tcggccagta
     8101 gttcggccag tgtgtagcgc acccgaccag tggaaacggc gcgggccggg tcggccttct
     8161 catgcagcct gttctctagg ttctttgggt ctgtcatcgg gcgacctcag caagcatgga
     8221 tactaggtgt tccaggtcag tgatcgaatg cgccaggaag cggccggcgc ggcaatctgc
     8281 aatcatttcc tcagtttttt cctactcatg ttcaacctcg ttcgtaccag ctcttcgagc
     8341 gattcaccac gcgcacgacc agcagcatca ccggcacttc gaccaggacg ccgaccacgg
     8401 ttgccaatgc agcgccggag tgcagaccga acaagctgat ggcggccgcc acggccagct
     8461 cgaagaagtt gctggcaccg atcagggccg aaggacaggc gacagagtgc ttttcccccg
     8521 cccgcttgtt cagccagtag gctaacccgg aattgaagaa cacttgaatc aagatcggca
     8581 cggctagcat ggcgatcact aacggctgcc ggatgatggc ctcgccctgg aaggcgaaca
     8641 gtagaaccaa cgtcagcagc agcgcggcca tcgaccacgg cccgatcttc tgcatggccc
     8701 gttcaaaggc ggcctgtcct tgcttgagca gatgccgccg caagagctgc gccaggatca
     8761 ccggcacgat gatgtagagc acgaccgaaa tcagcagcgt gtcccaaggc accgagatcg
     8821 aggacatgcc cagcagcagg ccgacgatgg gcgcgaaggc cacgatcatg atggcgtcgt
     8881 tcagagccac ttgcgagagc gtgaaaagcg ggtcgccgtt ggtaaggcgg ctccacacga
     8941 acaccattgc cgtgcagggc gcggcggcca gcaagatcaa gcccgcgatg tagctgtcca
     9001 gttgatcggc cggcaggaac ggagcgaaca cttgtcggat gaacagccac cccagcaggg
     9061 ccatcgagaa tggcttgacg gcccaattca cgaacagcgt gacgccgatg ccgcgccaat
     9121 gctctttgac ctggtgcagc gccccgaagt cgatcttgac cagcatcggg acgatcatta
     9181 cccaaatcag caggcccacc ggcaaattga cctgggccac ttccatgcgg cctatggcct
     9241 ggaacacgtc aggaaggccc tggccgagcg caacaccggc gacgatgcac agggccaccc
     9301 acagcgtcag gtaacgctcg aagccgctca tcggggcctg gcgcggcttc gcggcgatgg
     9361 cctgtgcgcc ggcgctcatg cttcctgctc ggtcttgccg atgttcgcca gctcgcgctt
     9421 gatggccgtc tggtcaagca ttttgagagg cagattcacg aacaggcgga cgcggttcat
     9481 catgtgccgg aaggtttgtt cgaaggcccg gcgcttctcg gcgtcagtgc cttcgacggc
     9541 ggccgggtct tcaaatcccc aatgcgccga gatcggctga ccaggccaca ccggacacat
     9601 ttcgccggcg gcgttgtcgc agacggtgat gatgaagtcc atcttcggtg cgtcgggcgt
     9661 ggcgtactcg tcccagctct tgctgcgcag attctcggtc gggtaattca ccgactccac
     9721 cttttcgacg gcgaagggat tgaccttgcc ggttgggtga ctgccggcgc tgtaggcgcg
     9781 gaagcgccct tgccccatcg tgttgatgag agcttcggcc atgatgctgc gcgccgaatt
     9841 gccggtgcag aggatgagga cgttatagat tctttcggtc atgatgtttc gctttctggt
     9901 agtggctggg gagcaatggc gatggatgaa agatcggtgg cctcgtcgat gacgtggccg
     9961 gccgctttcc gttcggaata gcggtctgtc agtgcttcac ggtgcggccg taccagcgcc
    10021 gtgaagcgca ccagttcttc catcacatcg gctattcggt cgtagtacgg cgagggcttc
    10081 atgcggcccg ccgcgtcgaa ctcttggaac gctttggcga tactcgactg gttcggaatg
    10141 gtgaacattc gcatccagcg gccgagcaga cgcaaggtgt tcacggcgtt gaagctctgc
    10201 gagccgccgg atacctgcat cacggccagg gtgcggcctt gggtcggccg gatgccggcc
    10261 atttcaagcg gcagatggtc aatctgcgcc ttcatgacac tggtaatctg accgtggcgt
    10321 tccgggctgc accagacttg tccctctgac cactcggaca gggcgcgcag ctccttgacg
    10381 gccgggtgat cgtcgctttg cacttgatcg ggcaacggca aatcggacgg gtcgaagatg
    10441 cgtgtttctg cgccaaagaa ttgcagcagc cgggccgctt cctcgacggc cagccgtgag
    10501 aacgagcggg cgcgcagcga gccatacagc agcaggatac gcaccggcgg ggcgtcgggt
    10561 gccagcccga gcgccgggcg ctcgatggca aaggatttgt ccaaagcggg caaagaatcg
    10621 gggtcggaaa gatggcgaag tctcatgcca acaccttccc ggcttccgtg gtgcaagaca
    10681 ggttgcacac ctggccgccg cagcagtttt ccgtgagaaa ggccaccagg ttagtggccg
    10741 tcgaaaagtt cgcctcgtag atcacaaatc ggccttcttg ccgcgacgtg accatgccgg
    10801 cgtgtgccag ctctttcagg tggaaggata gcgaagacgg cgggatgccg gccgcttcgc
    10861 tgatttttcc ggcggccatg ccggcggggc cggcctggac gagaagccgg aacaccgcga
    10921 ggcgcgattc ttgagcgatg gccgccaggg cggctacagc atttatcgtt tccatgtttc
    10981 aataatagtc gaaatatgga gtgtgctcaa tagctcattt aagctgtaaa aatgagctag
    11041 gcgcatttag atcgatatgt gataaattgg cctgccagtt taagatgggt gcatttgagt
    11101 atgcccaaag gagcccgcaa gtatgcgcag gacgaagcca gtagccgcgc cgatggtggc
    11161 gcgggtctat ctgcgcgtca gcaccgacgc gcaggacttg gaacgccaag aggcgatcac
    11221 tacggccgcg aaggccgccg gctactacgt cgccggcatc taccgtgaga aggcatccgg
    11281 cgcacgcgcc gaccggcctg agctgctgcg catgatcggc gacctacagc ccggcgaggt
    11341 ggtcattgcc gagaagatcg accgcatcag ccgcctacct ttgcccgagg ccgagcgcct
    11401 ggtggcctcg atacaggcca aaggcgcacg cctggccgtc cctggcgtgg tcgatctatc
    11461 cgacctggcg gccgaggccc agggcgtcgc caagatcgtg ctggaagccg tgcagatcat
    11521 gctttttcgc ctggccttgc agatggcccg cgacgactac gaggacaggc gcgaacgcca
    11581 gcgccaaggc attgagttgg cccgccaggc cgggcggtac aagggccgcc gtgctgatcc
    11641 gaagcgccgc gcccaagttg tcgcgctgcg caagtccggc tacagcatca acaagaccgc
    11701 cgagctggcc gggtacagtg cggcccaggt gaaacggata tgggccgagg tcagccaggc
    11761 cgaagcgaag cagcacggcg cgttcgtgga ggacgcattg acggaagccg atgccctggc
    11821 cgctgtcggc caggatgagc gccaggagga aagggcatga agaagccgaa ccaagacgac
    11881 gagccgtttt tcatcaccga ggagattgcg gccgaaatga tcgccggcgg ctatgagttc
    11941 gagctgccgc ccattccttg caccatccgc ctacgcgacg tgctggagcg catgaccgat
    12001 gctgagctag cattgcagcc gggcgagatc gccgaccagg agcgtgaacg ctgccggcgc
    12061 aagccgtgtt caacctcatg atctggtcat ggtatttttc atggcactga gcctgatagt
    12121 tcttgcaaat tgttgtcact aaagggtttt gtgtgcttgt ttacaatcga gtgggagtga
    12181 cgggcactgg ctggcaatgt ctagcaacgg caggcatttc ggctgagggt aaaagaactt
    12241 tccgctaagc gatagactgt atgtaaacac agtattgcaa ggacgcggaa catgcctcat
    12301 gtggcggcca ggacggccag ccgggatcgg gatactggtc gttaccagag ccaccgaccc
    12361 gagcaaaccc ttctctatca gatcgttgac gagtattacc cggcattcgc tgcgcttatg
    12421 gcagagcagg gaaaggaatt gccgggctat gtgcaacggg aatttgaaga atttctccaa
    12481 tgcgggcggc tggagcatgg ctttctacgg gttcgctgcg agtcttgcca cgccgagcac
    12541 ctggtcgctt tcagctgtaa gcgtcgcggt ttctgcccga gctgtggggc gcggcggatg
    12601 gccgaaagtg ccgccttgct ggttgatgaa gtactgcctg aacaacccat gcgtcagtgg
    12661 gtgttgagct tcccgtttca gctgcgtttc ctgtttgggg tcgtttgcgg gaaggggcgg
    12721 aatcctacgc taaggctttg gccagcgata ttctccggtg agattgatgt gttcccaggg
    12781 gataggagaa gtcgcttgat atctagtatg acgtctgtcg cacctgcttg atcgcggccg
    12841 cgatagctag atcgcgttgc tcctcttctc catccgcgtt ccaagctgcg gaaaggcacc
    12901 cataagcgta cgcctggtcg agcaggcgac gcggatcgac gtccagcgca cgagagaatg
    12961 cgtccgccat ctgtgcaatg cgtctaggat cgagacaaag gtcgtctctg tcagccggat
    13021 cgtagaacat attggcggcg ccaaagccca cttcaccgac cagaccgacg ggatctatca
    13081 ccagccagcc gcgactggag aacatgatgt tttcatgatg cagatcgcca tgtagcccac
    13141 gcagttccga ggcattgctc atcatttgat cggctataat cgccgcgtgg acgtagtcag
    13201 tttgacaacc tgcgttttga tcatcgcgcg cccgctgaaa caaagctgca aagcgatccc
    13261 ggatcgggag aagggcagaa ggcaggggtt cctcagatgc ggcatacagc ttcgccatta
    13321 gttccgctgc aatttcggtc gcctggtagt cgccgtgctc ggcaacgatg tgagagagca
    13381 ttcgctcccc ggcatattcg agcaacatca gattgttctc acgaccgagc aaccggactg
    13441 ctcccctccc attgcgccat accagatagt cggccccgcg cagttcatca gcaatgtctt
    13501 ctataggttt caatcccttg acgattgcag gagtcccgtc tggcaatgaa actttccaaa
    13561 cgaggctgga aaaggtgtcc gcaatgagaa caggttgcga aacgtgccaa tgagcaggaa
    13621 aaacaggcgg catgaacatc aaccccaagt cagagggtcc aatcgcagat agaaggcaag
    13681 gcgttcgcgg tcgggggctt cgatccccaa tacattgaat aggacagcga aggcgcgctc
    13741 tgcttcatct ggcgctgccc agttctcttc ggcgttagca atcatgagtg ccaaatcggc
    13801 atagcgatct gctgttccga gccgcccaag gtcgatcaga cccgtgcatt gaagagtttt
    13861 agggtccacc atgaagttcg gcatgcaggg atcaccatgg caaacaacca tatcggtgcg
    13921 ctcttggtcg agccgcaccg gtagctctcg ttcgacacga gccaaaagat cgagctgcgg
    13981 cgtactcttg tcctcgtccg gtaagaagtc gggattgacg gcattgcggg acaccacatc
    14041 aacggcgcgt ccgaacattc gcgacagcct gcgctcaaac ggacattgat caaccgatag
    14101 gctgtgaaca gcgccaagtt gctgccccat tgacggccac gctttgagca aatccgctcc
    14161 agacagatca gccgccggta ctcccggaat tgccgttatc accaagcatg caccctcctg
    14221 ttcctcctgc cagttgatga cctcggggca agccacacct cgacctttga gccaaatgag
    14281 gcggtcacgc tctccagcga gctcaccgcg gcgggaagca ggtgcgattt tcgcgaaggc
    14341 atgcccgtca ccacgtcgaa aaacaaaatc accagattct ccgcctctga caggcaacca
    14401 gtcagaatgc gattcaccaa aaaaaatatt agttcgattc aatggaggtt ccttcagttt
    14461 tctgatgaag cgcgaatata gagaaatatc ccgaatgtgc agttaacgaa ttcttgcggt
    14521 ttctttcagc gccgccaata ccgccagccc gtcgcgcaag gggcgcggct cgtgtgtgcg
    14581 gatgaagtca gctccacctg cggcggcggc aagctctgca gcgagtgtcg cggccccgac
    14641 atcccccgga ccacggcctg tgagcgcgcg cagaaaggat ttgcgcgaaa cagacagaag
    14701 caccggcaaa tcgaagcgca gccgcaattc atcgaaccgc gccagcaccg agagcgaggt
    14761 ttcgggagca gcccccagaa aaaaccccat gccgggatca aggacaaggc ggttgcgttt
    14821 gataccggca cccgtcagcg ccgcgatgcg cgcgtcaaag aacgccgcaa tgtgatccat
    14881 gatgtcgcca gcgggtgcct cgcgccgatc tgcctgcccg tcttgcaccg aatgcataac
    14941 gacgagtttg gcagatgatt tcgccaattg cggatagaac gcagcgtctg gaaaaccgcg
    15001 aatatcattg agataggcca caccacgcga caaggcatag gcttgcgtcg cgggttgata
    15061 actgtcgagc gagacgggaa tgccatctgc cttgagcgcg tccagcaccg gcgcgatacg
    15121 cgcgatttct gtgtcggacg aaacaggcgc ggcgtcggga ttgctggatg ccggaccgag
    15181 gtcgatcaca tctgccccct cggccatcag cttacgcgcc tgcgcaatgg ctgcgtctgg
    15241 cgccagatac cggcctccat cggagaaact gtccgaggtt atgttgacga tgccgaaaat
    15301 gatgagcgat ttattcatgg gggcttctat aataataata atcgagcatg agtctcatac
    15361 ggatgctcgg gtcgaaaggg aatccccagg cgagtaacct gtttgcggtg atccattagc
    15421 tgcaggagca gaatagcata catctggaag caaagccagg aaagcggcct atggagctgt
    15481 gcggcagcgc tcagtaggca atttttcaaa atattgttaa gccttttctg agcatggtat
    15541 ttttcatggt attaccaatt agcaggaaaa taagccattg aatataaaag ataaaaatgt
    15601 cttgtttaca atagagtggg a
//
