LOCUS       Tn6538                  4549 bp    DNA     circular     13-JUN-2018
DEFINITION  Vibrio alginolyticus.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Vibrio alginolyticus
  ORGANISM  Vibrio alginolyticus
            Unclassified.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Zhou,D.
  TITLE     Compelete sequence of Tn6538
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2018) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v.8.5.1
            Sequencing Technology :: Illumina; PacBio
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Vibrio alginolyticus"
                     /plasmid=""
                     /mol_type="genomic DNA"
     mobile_element  1..1656
                     /gene="ISEcp1"
                     /product="Insertion sequence: ISEcp1"
     repeat_region   1..14
                     /gene="IRL_ISEcp1"
                     /product="ISEcp1 inverted repeat left"
     CDS             187..1449
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISEcp1 transposase A"
                     /translation="MINKIDFKAKNLTSNAGLFLLLENAKSNGIFDFIENDLVFDNDS
                     TNKIKMNHIKTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNFK
                     TTQMFRDINFKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYN
                     IQFAFCDELKAYVTGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEK
                     IIETIESLGCKYLIKAKSYSTLTSQATNSSIVFVKGEEGRETTELYTKLVKWEKDRRF
                     VVSRVLKPEKERAQLSLLEGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKY
                     DMAVGHLLLKSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAA
                     KIIKTARYVIMKLSENYPYKGVYEKCLV"
     repeat_region   1643..1656
                     /gene="IRR-1_ISEcp1"
                     /product="ISEcp1 inverted repeat right-1"
     CDS             1773..2918
                     /gene="blaCMY-2"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase CMY-2"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQP
                     QWTPGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKD
                     YAWGYREGKPVHVSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
     CDS             3012..3545
                     /gene="blc"
                     /codon_start=1
                     /transl_table=11
                     /product="Outer membrane lipoprotein Blc"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIAT
                     REGFEVNKLIWVKQPGA"
     CDS             complement(3542..3982)
                     /gene="sugE"
                     /codon_start=1
                     /transl_table=11
                     /product="Quaternary ammonium compound-resistance protein
                     SugE"
                     /translation="MNKKVEIHFLPGKNAISHISFAGRPANASFTGDGPIVLEPDMSW
                     IVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIAMLSWAMRTLPVGTAYAV
                     WTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKLSTH"
     CDS             4116..4541
                     /gene="orf426"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /translation="MLKILVIDRCHFTRTGIEALLNHSGRFSSSFLVSGINNLLLAKE
                     HILQWKPHLVIADLYSFISETHSSPPIKPFFMSCGVIPLILLQSADRQHAPIVPSQSV
                     AHSVLTKHTTLNTLSHTIQDFLICSKRALPQPMFMHSLP"
     repeat_region   4536..4549
                     /gene="IRR-2_ISEcp1"
                     /product="ISEcp1 inverted repeat right-2"
BASE COUNT     1364 a   1018 c   1000 g   1167 t
ORIGIN
        1 cctagattct acgtcagtac ttcaaaaagc ataatcaaag ccttgataaa tatgcattcc
       61 ttcgaaattc agctttcacc cattgggtga aagaaaagtg ctcaaaaata tgttaaatta
      121 tcagctttta tgactcgata tatggtaaaa taatagtaag aaaagtagta aaaaggggtt
      181 ctaattatga ttaataaaat tgatttcaaa gctaagaatc taacatcaaa tgcaggtctt
      241 tttctgctcc ttgagaatgc aaaaagcaat gggatttttg attttattga aaatgacctc
      301 gtatttgata atgactcaac aaataaaatc aagatgaatc atataaagac catgctctgc
      361 ggtcacttca ttggcattga taagttagaa cgtctaaagc tacttcaaaa tgatcccctc
      421 gtcaacgagt ttgatatttc cgtaaaagaa cctgaaacag tgtcacggtt tctaggaaac
      481 ttcaacttca agacaaccca aatgtttaga gacattaatt ttaaagtctt taaaaaactg
      541 ctcactaaaa gtaaattgac atccattacg attgatattg atagtagtgt aattaacgta
      601 gaaggtcatc aagaaggtgc gtcaaaagga tataatccta agaaactggg aaaccgatgc
      661 tacaatatcc aatttgcatt ttgcgacgaa ttaaaagcat atgttaccgg atttgtaaga
      721 agtggcaata cttacactgc aaacggtgct gcggaaatga tcaaagaaat tgttgctaac
      781 atcaaatcag acgatttaga aattttattt cgaatggata gtggctactt tgatgaaaaa
      841 attatcgaaa cgatagaatc tcttggatgc aaatatttaa ttaaagccaa aagttattct
      901 acactcacct cacaagcaac gaattcatca attgtattcg ttaaaggaga agaaggtaga
      961 gaaactacag aactgtatac aaaattagtt aaatgggaaa aagacagaag atttgtcgta
     1021 tctcgcgtac tgaaaccaga aaaagaaaga gcacaattat cacttttaga aggttccgaa
     1081 tacgactact ttttctttgt aacaaatact accttgcttt ctgaaaaagt agttatatac
     1141 tatgaaaagc gtggtaatgc tgaaaactat atcaaagaag ccaaatacga catggcggtg
     1201 ggtcatctct tgctaaagtc attttgggcg aatgaagccg tgtttcaaat gatgatgctt
     1261 tcatataacc tatttttgtt gttcaagttt gattccttgg actcttcaga atacagacag
     1321 caaataaaga cctttcgttt gaagtatgta tttcttgcag caaaaataat caaaaccgca
     1381 agatatgtaa tcatgaagtt gtcggaaaac tatccgtaca agggagtgta tgaaaaatgt
     1441 ctggtataat aagaatatca tcaataaaat tgagtgttgc tctgtggata acttgcagag
     1501 tttattaagt atcattgcag caaagatgaa atcaatgatt tatcaaaaat gattgaaagg
     1561 tggttgtaaa taatgttaca atgtgtgaga agcagtctaa attcttcgtg aaatagtgat
     1621 ttttgaagct aataaaaaac acacgtggaa tttaggaaaa acttatatct gctgctaaat
     1681 ttaaccgttt gtcaacacgg tgcaaatcaa acacactgat tgcgtctgac gggcccggac
     1741 acctttttgc ttttaattac ggaactgatt tcatgatgaa aaaatcgtta tgctgcgctc
     1801 tgctgctgac agcctctttc tccacatttg ctgccgcaaa aacagaacaa cagattgccg
     1861 atatcgttaa tcgcaccatc accccgttga tgcaggagca ggctattccg ggtatggccg
     1921 ttgccgttat ctaccaggga aaaccctatt atttcacctg gggtaaagcc gatatcgcca
     1981 ataaccaccc agtcacgcag caaacgctgt ttgagctagg atcggttagt aagacgttta
     2041 acggcgtgtt gggcggcgat gctatcgccc gcggcgaaat taagctcagc gatccggtca
     2101 cgaaatactg gccagaactg acaggcaaac agtggcaggg tatccgcctg ctgcacttag
     2161 ccacctatac ggcaggcggc ctaccgctgc agatccccga tgacgttagg gataaagccg
     2221 cattactgca tttttatcaa aactggcagc cgcaatggac tccgggcgct aagcgacttt
     2281 acgctaactc cagcattggt ctgtttggcg cgctggcggt gaaaccctca ggaatgagtt
     2341 acgaagaggc aatgaccaga cgcgtcctgc aaccattaaa actggcgcat acctggatta
     2401 cggttccgca gaacgaacaa aaagattatg cctggggcta tcgcgaaggg aagcccgtac
     2461 acgtttctcc gggacaactt gacgccgaag cctatggcgt gaaatccagc gttattgata
     2521 tggcccgctg ggttcaggcc aacatggatg ccagccacgt tcaggagaaa acgctccagc
     2581 agggcattgc gcttgcgcag tctcgctact ggcgtattgg cgatatgtac cagggattag
     2641 gctgggagat gctgaactgg ccgctgaaag ctgattcgat catcaacggc agcgacagca
     2701 aagtggcatt ggcagcgctt cccgccgttg aggtaaaccc gcccgccccc gcagtgaaag
     2761 cctcatgggt gcataaaacg ggctccactg gtggatttgg cagctacgta gccttcgttc
     2821 cagaaaaaaa ccttggcatc gtgatgctgg caaacaaaag ctatcctaac cctgtccgtg
     2881 tcgaggcggc ctggcgcatt cttgaaaagc tgcaataact gacgatgagg cccaggatat
     2941 tgggcctcct ttctttctct ttttttcctg ttgtcatcta cacttaacaa aaatacagca
     3001 aggaaaatcc catgcgcatt ttgcccgtcg ttgctgcagt tacggctgca ttcctggttg
     3061 tcgcgtgtag ctccccgaca ccgccgaaag gcgttaccgt ggtaaataac tttgatgcca
     3121 aacgctatct gggaacctgg tatgaaattg cgcgcttcga ccatcgtttc gagcgcggat
     3181 tggataaagt gaccgcaaca tacagcttgc gcgacgacgg cggcatcaac gttattaaca
     3241 agggctataa ccctgacagg gagatgtggc agaaaacgga agggaaagcc tatttcaccg
     3301 gcgacccaag cagagccgcg cttaaggttt cttttttcgg ccccttctat ggcgggtata
     3361 acgtaattgc actcgaccgg gaatatcgtc acgcgctggt ttgtggtccg gatcgcgact
     3421 acctgtggat cctttcacgg acccctacta tttcagatga aatgaaacag caaatgttag
     3481 ccatcgcgac ccgggaaggg tttgaagtga ataaactgat ttgggtgaaa cagcctggcg
     3541 cttagtgagt gctcagcttc agaccaataa tgccagcaac gatcagccca aggctcagca
     3601 aacgtgccgg gctggcagac tcacccagca gcaaaatccc tgtaatggcc gccccaacag
     3661 cgccaatacc ggtccagacc gcataagcgg ttcctacagg caacgtgcgc attgcccaag
     3721 agagcatggc gatactgacg atcatcgccg caatagtgat aatgcttggc gtaagacgcg
     3781 taaaaccgtg ggtgtatttc aggccaatcg cccagacaac ttcgagcaaa cctgcaatta
     3841 ataaaacgat ccaggacata tcaggctcca gaacaatggg gccgtccccg gtgaaagaag
     3901 cgtttgcagg tcgtcctgca aagctaatgt gtgaaatggc atttttgccc ggaagaaaat
     3961 gaatttcaac ctttttattc accgcctgct aaaagcaaga attaagcata attagcggcg
     4021 tagttcccgc atttggcact catcggagtt ctctatgatg aagggctttc tgaaaccaga
     4081 aagtcgattg cccctcttct gaatgcgaaa cgtttatgct taaaatttta gtgattgacc
     4141 ggtgccactt tacccgcaca gggatagagg ccttgcttaa tcattctggc aggttcagct
     4201 cctcatttct ggtatcagga atcaataatc tcctgttagc aaaagagcat attttgcaat
     4261 ggaaaccgca tctggtgatc gcggatttat acagttttat tagcgagacg cactccagtc
     4321 cgccaattaa accttttttt atgagttgtg gcgtcatccc actaatttta ctgcaatcag
     4381 cagacagaca gcatgcaccc atcgtgccct ctcaatccgt agcccattcg gtcttgacca
     4441 agcacaccac gctgaacaca ctctcccaca caattcagga tttccttatt tgctcaaaaa
     4501 gggcacttcc acagcccatg tttatgcact ccctcccgta acctccagc
//
