LOCUS       Tn6519                 16806 bp    DNA     linear       29-SEP-2020
DEFINITION  Acinetobacter baumannii.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Unclassified.
REFERENCE   1  (bases 1 to 16806)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn6519
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16806)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..16806
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
     repeat_region   1..26
                     /gene="IRL_Tn6519"
     repeat_region   8..26
     repeat_region   86..104
     repeat_region   117..135
     CDS             156..866
                     /gene="tnsA"
                     /codon_start=1
                     /product="Tn6519 endonuclease"
                     /translation="MKKLSDEYLPVRKAQTVYGSISGNYAFRGEKTIWFESTLERDFI
                     LKQEFNNNVIDVIGQPVVIPYITELGNQSTYTPDFLVQFSSSNCDDFEDFPVPMLIEI
                     KPKKKLVEDWDKLKPKFRAAHRFAADKGWKFKIISETRLYDQYWENINFLKKFRRSHV
                     GAVDQQLLLDTLQKLGQSTINQLPAHIFKDKTNVLKGIHQVWALVAKRMIACDLYCPL
                     TAETVIWVNQNDAFARNI"
     CDS             867..2777
                     /gene="tnsB"
                     /codon_start=1
                     /product="Tn6519 transposase"
                     /translation="MIESNAVNIDRSRIILKPNVVVGYEGQPYKIVNVLNANDIVISS
                     LDSVRSLQVNARSLTIFNGGNTLTERLNKGDQDISNEAWQIALQRYEIIKPLIEYSTT
                     ELVENRANEYDVNRSTLWKWLKDYRENNSLMALIPKKRGWTTEKSRLSPQVSNIIKQA
                     INDEYLNAKKPSISKTIEIVKAECSRLQLEAPHENSIRRRIEALNDYQVTKARLGSKA
                     AIDKFKAAAGSFPNADYPLAYVQIDHTPLDIEIVDDEFREAIGKPHLTLAIDVFSRMI
                     VGYYLSLEAPSTTSVAMCIASCILSKKRKLIELDIDAEWQVEGIMDSVHTDNGPDFRT
                     NHISKACLKYGIHWEYRPIGGARFGGHIERMLGIVNLEMHVLDGTTFSNVQQRGTYDS
                     AKQACMTLKELEYYIVYWITKVYHQKKHSALGTSPIVKWEEGVWGTKTTAGTGLKERV
                     SDEDTLFIDFLPEFEATIQRTGVQKDNLFYFADCLRQWVNSIDPEDNNRKRKRKFLFK
                     RDPRDISQIWFYEPYSNTYFKVPTAKREIPPISLFEYKQVQNYLKSERQDVQNQDEIY
                     KAILHLRDQLNQARSLTRKQRRSNQRKKENAKAITQLSEQNQSKKAVVSESLQTSDDL
                     WSTPLTAFDDLR"
     CDS             2782..3702
                     /gene="tnsC"
                     /codon_start=1
                     /product="Tn6519 transposition regulator"
                     /translation="MEKYEHLAEHVLEIMQLSDSERIETLFTDRWIGYKKAITIMNTL
                     TDILNRPRKLRPECLLIVGDSNMGKTTIIHEFARQYYTKTVSDADMDLLSVTKPVLPI
                     LAPAKANVKELYINILNHFFVPFRASDPEAKLRNQAVHLMRKYETKMLIIDEIHNCLT
                     GSAKLLPEVMNTLKNLSNELSLNIVGVGTREAITILHTDPQYASRFDVVNLPKWELNQ
                     DFLRLLVSYVRLLPLKKQSNLASKEIATLIFEVSGGNLGDLNRLLVECAKEAILQGDE
                     EITYDIVHKFKWLKPTEGLRNIRNINLSLS"
     CDS             3705..4847
                     /gene="tnsD"
                     /codon_start=1
                     /product="Tn6519 target-site selection protein"
                     /translation="MRNHYREVEMSQPEQCWYIRTPIQQGEIFSSWLIRSALDVGCSP
                     MVLIEALWGKWRALTIDLDKGVDAERFDALLSHSMESKQKIQQSMLSSVVSQIQPNYD
                     SNQNIPWVLSLGTRNRSNTSGRQVCVECLKSHENPPYLRLMWRIGWHCSCVEHQLSLI
                     DHCPECGVTIQPFKADMEHGCLAICTTCGFDLRRCEESKNINLNALNFQNKAEQVLKQ
                     KIGFYNQSPVTTQVWFEIARSWLSEIRFLVNTPNKNVIQLFESFDVNLHLSHPVTPLA
                     FEYLSTQERIVLLSILDQIMDIPCDLLVQRSKEYGVSRANFWDKRKKLPVQLQQMKDL
                     MIKPTRRYPVSRAAITVTKPKSKATVQRQWLNLLRRSNNSGAMHID"
     CDS             4825..6270
                     /gene="tnsE"
                     /codon_start=1
                     /product="Tn6519 target-site selection protein"
                     /translation="-
                     ERCILTENVIEHDCHGCNQSVSFIKKRYKGKKYCSTCYARIFKKRLCPSCGDFARLPR
                     DDEQAICNECIKKQPCIRCNQTNKPIGKLTEYGVVCNSCSVYFRPIEPCERCGTPSQK
                     LTRISPFNDDLRVCPKCATRDYETCPSCQKHRLLESDVSGQRTCKKCRDKPQKSCKAC
                     HCMIAAGCADLCDDCYWHQNLWNKFDQNQKVFESSDLKQQYENYIGWLEKKVGSHKAA
                     LYINKHTHFFIKTEIDWNQSVPTPKQLLVRLRSSGLRKFELVMQWLEEVHDIRIDMDN
                     KKSCSERDQMEKLVQRILQPSLAYDVVLEYKNKLEEKIKRGETSIRSARLAVKPAVAL
                     MLSMEGESAQLPNLEHVKAYLAEYSGQAAALTGFINFLNENYGASIDYLKLKKSDFLK
                     TKQKKKLEMELIALTQTDLNDSELILSWVRNGLRYFHQLPYIDALKIKTEMITEIEDG
                     FTVVLNGQYYWLPKTQ"
     CDS             complement(6645..7016)
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MGQAKQRGTLEQRVKQAQEQKDDFYGTERSVAEVLAELGLPKES
                     TVKGYVIHLPEKDEFVAAINDNNISFSVAYARTPELAMIYDEPQKAISDAKKISKHKL
                     LVCVLFETSNQYMINDIWANY"
     CDS             complement(7456..8301)
                     /gene="uspA"
                     /codon_start=1
                     /product="Universal stress protein UspA"
                     /translation="MSKIIACIDGSLVTNTVCDYAAWFSDKLNSPIKLLHVIDKPKAP
                     QDLSGAIGLGSRETLLKELVELEERKGKIELEHGQILLREAKNYLLEKFSIDAQSFQR
                     HGSVLETIMGMEDDIRVLVMGKHGNETEHDSSKIGTHIENVVRALHKPVLITSAPFSP
                     PKSFLIAFDGSQTARKCVERIASSPLLKGLAVHLVYVGNPNSEMQNQLSWAKEQLESQ
                     GFNITSNTLDGEVDKAIINYAEQHQIDLIVVGAYGHSKIRQFFIGSTTTKVITSANKP
                     VLLLR"
     misc_feature    complement(8314..9765)
                     /gene="truncated sup-5'"
                     /product="Truncated sulphate permease, 5' fragment
                     (pseudogene)"
     repeat_region   9757..9765
                     /gene="DR_Tn2006"
     repeat_region   complement(9766..9780)
                     /gene="IRR_ISAba1"
     CDS             complement(9784..10230)
                     /gene="tnpB"
                     /codon_start=1
                     /product="ISAba1 transposase tnpB"
                     /translation="MVGRVKLYISALQLENGELLLVVSPQFNANAIQDYALRWEIETL
                     FSCLKGRXFNLENTRLTDPRRVKKLIAVLAISFCWCYLTGEWQHDQKKAIKIKKHGRL
                     SMSLFRYGLDYVQMAIQRLIGFGKKEEFKEILAILRRQNPDRIRVL"
     CDS             complement(10305..10874)
                     /gene="tnpA"
                     /codon_start=1
                     /product="ISAba1 transposase tnpA"
                     /translation="MTHLNELYLILNKSLKWNKSHLKCFALIMLVIILKQTCNLSSAS
                     KALPIKCLPQSFYRRMQRFFAGQYFDYRQISQLIFNMFSFDQVQLTLDRTNWKWGKRN
                     INILMLAIVYRGIAIPILWTLLNKRGNSDTKERIALIQRFIAIFGKDRIVNVFADREF
                     IGEQWFTWLIEQDINFCIRVKKTSLSPII"
     repeat_region   complement(10931..10945)
                     /gene="IRL_ISAba1"
     CDS             10976..11308
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MVWVKTIAGKLEERIRYTSAICYNTFPVPKLMKASIFKLNESAF
                     KILAVRESYSHLSLAQLYDPEKMPFDLKQAHKENDSLVEKLYKSSDFKTDEERLERLF
                     HYYETMLN"
     CDS             11316..11870
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MKNLVEVNYQQTGESTSVNNMGMRAMQARAFESRNSQYLLLKAP
                     PASGKSRALMFLGLDKIHNQGLSKVIVAVPERSIGGSFASTKLSESGFFADWDIKPEN
                     NLCTAGGDKSKVKAFKHFMEGTDQILVCTHATLRFAFEELEDHVFNNTLVKIHDNFCR
                     ICQPMMFSLLVHAAQENLQLSVPF"
     CDS             12122..12430
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MHENTPVTRVDVPQYTELHPQEAIEEKISLSDRFGMWLSFYPMD
                     QNLYLTIVEHYLAKTDMPMNDEAHAEALRWCQARGQRSGRAAYQFSKHWIGSQQLKAL
                     "
     CDS             complement(12535..13356)
                     /gene="blaOXA-23"
                     /codon_start=1
                     /product="Class D beta-lactamase OXA-23"
                     /translation="MNKYFTCYVVASLFLSGCTVQHNLINETPSQIVQGHNQVIHQYF
                     DEKNTSGVLVIQTDKKINLYGNALSRANTEYVPASTFKMLNALIGLENQKTDINEIFK
                     WKGEKRSFTAWEKDMTLGEAMKLSAVPVYQELARRIGLDLMQKEVKRIGFGNAEIGQQ
                     VDNFWLVGPLKVTPIQEVEFVSQLAHTQLPFSEKVQANVKNMLLLEESNGYKIFGKTG
                     WAMDIKPQVGWLTGWVEQPDGKIVAFALNMEMRSEMPASIRNELLMKSLKQLNII"
     repeat_region   13391..13405
                     /gene="IRL_ISAba1"
     CDS             13462..14031
                     /gene="tnpA"
                     /codon_start=1
                     /product="ISAba1 transposase tnpA"
                     /translation="MTHLNELYLILNKSLKWNKSHLKCFALIMLVIILKQTCNLSSAS
                     KALPIKCLPQSFYRRMQRFFAGQYFDYRQISQLIFNMFSFDQVQLTLDRTNWKWGKRN
                     INILMLAIVYRGIAIPILWTLLNKRGNSDTKERIALIQRFIAIFGKDRIVNVFADREF
                     IGEQWFTWLIEQDINFCIRVKKTSLSPII"
     CDS             14106..14552
                     /gene="tnpB"
                     /codon_start=1
                     /product="ISAba1 transposase tnpB"
                     /translation="MVGRVKLYISALQLENGELLLVVSPQFNANAIQDYALRWEIETL
                     FSCLKGRGFNLGNTRLTDPRRVKKLIAVLAISFCWCYLTGEWQHDQKKAIKIKKHGRL
                     SMSLFRYGLDYVQMAIQRLIGFGKKEEFKEILAILRRQNPDRIRVL"
     repeat_region   14556..14570
                     /gene="IRR_ISAba1"
     repeat_region   14571..14579
                     /gene="DR_Tn2006"
     misc_feature    14580..14615
                     /gene="truncated sup-3'"
                     /product="Truncated sulphate permease, 3' fragment
                     (pseudogene)"
     CDS             complement(14910..16706)
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MPVNNDKITTIDCFPNDNISRVVYRYGGLVRNEDNDSTNPFVEV
                     LLIEIRKSDQWLFLDKCSTFLVPVLDLDAVQHGSIWDGNVLTDQSYRFSGKLITKRFS
                     FDFTNNKPKNIKLTDRIPNTSDYYIPLKNYFLPNKVDYVLQGYPFTKYTNDSYRKVNH
                     CLMHSNDGTQVITSSIHVLHSLFVNRKDIRGLLLGTSSQSIINRFLESYTTEIVDDNV
                     EYKIKIRKPYEDIGETAIIFLANLALNPYVQTIVDKIQRSMEITEFNHLQQGTGKRYP
                     IVFPPHPTKLFLEAEGIWLDDNKTRFFITRVKKFDPINDHMIDVNKDLTNTISKPDEK
                     NPRPREKSQNKNEHINTQKPPSRTSGEYRKRSEVETGNTQGILKYSFNEPTDDPIEVG
                     ASNQPYSDKSEEVETSSDEPYGNQNTKIKKSETTDTPPSRDERFDIQYIIQSLQELAS
                     EMGSPLEHLSAINEHGEMIASINLLQIKKLVPEPKHPSWIDYDKGRKLLFLKLDLKDQ
                     NGFSYLIDIHKNKNHEAFCAFLIFTQNKLTSEQIKKICIELENAKGIKKWALYCDSFI
                     HKMIAIKHLYATPDEWKNRFKNLFISLQKDKS"
     repeat_region   16761..16779
     repeat_region   16781..16806
                     /gene="IRR_Tn6519"
     repeat_region   16781..16799
ORIGIN      
        1 tgtcatttac agcaatagaa tagagtggca ttatagataa tcaataggtt gtcactctaa
       61 tttcacacaa ttaaattaga ttatttacat aaatagataa gagtgacttg caaaattaca
      121 ttaataggca cgagtgcctg aatggtgact tttttatgaa aaaattgagt gacgaatact
      181 tacctgttag aaaagctcaa actgtttatg ggagtatttc gggaaattat gcctttagag
      241 gtgagaaaac catttggttt gaatcgaccc ttgagcgtga tttcatactt aagcaagaat
      301 ttaataacaa tgtgattgat gttatcggtc aacctgtagt tatcccctac atcactgaat
      361 tgggtaacca atcaacctac actcctgact ttttagtgca gttttcaagc tcgaattgtg
      421 atgattttga ggattttcct gtaccaatgc tcatcgaaat taagcctaag aaaaagcttg
      481 ttgaggactg ggataagtta aaaccaaaat tcagagcagc ccatcgtttt gctgctgata
      541 aaggatggaa gttcaaaatt atcagtgaaa caagattata tgatcagtat tgggaaaata
      601 ttaatttctt aaaaaaattt agacgctctc atgtaggtgc cgttgatcaa caactattac
      661 tcgataccct acaaaagtta ggtcaatcaa caattaatca attacctgcc catattttta
      721 aagataaaac caatgtttta aaaggaattc accaagtttg ggcattagtg gcaaagcgca
      781 tgattgcttg tgacttgtat tgccctttaa cggctgaaac agtgatatgg gtgaaccaaa
      841 atgatgcatt tgcgaggaat atctaaatga ttgaatctaa tgctgtcaat atcgatcgta
      901 gtcgtatcat tctaaaaccc aatgtggttg tcggttatga ggggcaaccc tataaaattg
      961 tgaatgtttt aaatgccaat gacattgtta tatcaagtct tgattcggta cgtagtctac
     1021 aggtcaatgc tagaagtttg accattttta atgggggaaa tacgctaact gagcgtttga
     1081 ataagggtga tcaggatatt agtaatgaag cttggcagat tgctttacaa cgttatgaga
     1141 ttattaagcc tttgattgag tattcaacga ctgaactggt cgaaaatagg gcgaatgaat
     1201 atgatgtgaa tcgctcaaca ttgtggaaat ggcttaaaga ttatcgggaa aataattctc
     1261 taatggcatt gattcccaaa aaacgaggtt ggacaactga aaaatcaagg ttgtcacctc
     1321 aagtgagcaa tatcattaaa caagctatca atgatgaata tttgaatgcg aagaaaccca
     1381 gtatttccaa aacgattgaa attgtaaaag cagagtgttc tcggcttcag cttgaagcac
     1441 cgcatgaaaa ctctattcgt agaagaatcg aggctttaaa tgattaccaa gtaacgaaag
     1501 cacgtcttgg atctaaagct gcaatagata aatttaaggc tgctgctggt tcatttccaa
     1561 atgctgatta ccctttggca tatgtgcaga ttgatcatac acctctagat attgaaatcg
     1621 tggatgacga gtttagagaa gcgattggaa aacctcattt aaccttggca atcgatgttt
     1681 tcagtcggat gatcgtaggg tattacctat cactagaagc gccaagtacg acttcagtgg
     1741 cgatgtgtat cgcatcttgt attttgtcaa aaaaacgtaa gttgatcgaa ctcgatattg
     1801 atgctgaatg gcaggtcgaa gggattatgg actcggtgca tacagataat ggccccgatt
     1861 ttagaaccaa tcacatcagt aaagcttgtc taaagtatgg gattcattgg gaataccgac
     1921 caattggtgg ggcaagattt ggcggtcata tcgagcgtat gcttggtatc gtcaatcttg
     1981 aaatgcatgt cttggatggt acaacttttt caaatgtaca gcagcgtggg acttatgatt
     2041 ccgctaaaca agcctgtatg acattaaaag agcttgaata ctatatcgtg tattggatta
     2101 ctaaagttta tcaccagaag aaacattccg ctttaggaac atccccaatc gttaaatggg
     2161 aagaaggtgt ttgggggacg aagacgacag caggtacagg tttgaaagaa cgtgtatcag
     2221 atgaagatac cttgtttatt gattttctac ctgaatttga agcgactatt cagcgtacag
     2281 gggtgcaaaa agataactta ttctattttg ccgactgttt aagacaatgg gttaattcta
     2341 ttgatccaga ggacaataat cgaaagcgca aaagaaaatt cttgtttaaa cgtgatccga
     2401 gggatatttc acagatttgg ttttatgagc catattctaa tacctatttt aaagtaccta
     2461 ccgcaaagcg agagatacca ccgattagct tatttgaata taagcaggtt cagaactatc
     2521 tcaaaagtga acgtcaggat gttcagaatc aggatgagat ttacaaggcg attttgcatc
     2581 tacgtgatca acttaatcag gctagaagct tgactcgaaa gcaacgtcgc tcaaaccaaa
     2641 gaaaaaagga aaatgcaaaa gccattactc agctttccga acagaatcag tctaaaaagg
     2701 ctgtagtttc tgaaagtctt cagacaagtg atgatttgtg gagtactcct ttaactgcat
     2761 ttgacgattt aaggtgatgc aatggaaaaa tatgaacatc ttgcagagca tgtacttgaa
     2821 atcatgcaat taagcgatag tgaacggatt gagacattat ttactgatag atggattggc
     2881 tataagaaag ccattaccat catgaacaca ttgaccgata ttttgaatcg ccctaggaaa
     2941 ttaagacctg aatgcttgtt gattgttggt gactccaata tgggcaaaac aactatcatt
     3001 catgagtttg ctagacagta ttatacaaaa accgtaagtg atgctgacat ggatttattg
     3061 tccgtaacta agccagtttt accgatactt gctccagcaa aagccaatgt taaagaactc
     3121 tatattaata ttttaaatca cttctttgtg ccatttcggg caagtgatcc tgaagccaaa
     3181 ttacgaaatc aagccgtaca cttgatgcgt aagtatgaaa caaaaatgtt gattatcgat
     3241 gaaattcata attgtctgac aggttcagcc aaattgcttc ccgaagtcat gaatacgctc
     3301 aagaacctca gcaatgaatt gagtctgaat atcgtaggtg ttggtactcg tgaggcaatc
     3361 accatactgc atactgatcc gcaatatgcc agtcgctttg atgtggtgaa tttacctaaa
     3421 tgggaactca atcaagattt tttaagatta ttagtcagct atgttcgact attgccgtta
     3481 aaaaaacagt ctaatcttgc atccaaagag attgctacgt tgatttttga agtgtcaggt
     3541 ggaaatttag gggatttaaa tcgattactg gtggagtgtg ccaaagaagc cattttacaa
     3601 ggtgatgaag aaattaccta tgacatcgta cataagttta aatggcttaa acctacggag
     3661 ggtttaagaa atattagaaa tataaatctc agcctgagtt aattttgcga aatcattata
     3721 gggaggtaga gatgagtcaa cctgaacaat gttggtatat cagaactccc attcagcaag
     3781 gggaaatatt ttcatcttgg ttgatccgtt cagccttaga tgtaggttgc tcacctatgg
     3841 ttctcattga ggctttatgg gggaaatgga gagctttaac gattgattta gacaaaggag
     3901 tagatgccga aagattcgat gctctgttat ctcatagtat ggagtcaaaa cagaagatac
     3961 agcaatcgat gttgagtagc gtcgtttctc agatacagcc aaattatgat tcaaatcaaa
     4021 atatcccttg ggttttatcc ttgggcacac gaaaccgctc aaatacatca ggtcggcaag
     4081 tatgtgttga atgcctgaag agtcatgaaa atccgcctta tttgcgctta atgtggcgta
     4141 ttgggtggca ttgtagttgt gttgagcatc agttaagttt aattgaccat tgccctgaat
     4201 gtggtgtgac gatccaacca tttaaagccg atatggaaca tggttgttta gcgatctgta
     4261 cgacatgtgg ctttgattta aggcgttgtg aggaaagcaa aaacatcaat ttaaatgctt
     4321 taaacttcca aaataaagca gagcaagtct taaaacaaaa aattggtttt tataatcaat
     4381 ccccagtgac tactcaagta tggtttgaaa ttgctcgttc atggctcagt gagattagat
     4441 ttttggtcaa tacgcccaat aaaaatgtga ttcaattatt tgaaagtttt gacgtaaatt
     4501 tacatctttc acatccagtt accccattag catttgaata tctgagtact caggaacgaa
     4561 tagttttgct ttcaatattg gatcagatta tggatattcc atgtgactta ttggtacaac
     4621 gatcaaagga atatggagta agtcgtgcaa atttttggga caagcgcaag aagttgcctg
     4681 ttcagcttca acagatgaaa gatttaatga ttaagcctac aagacgttat cctgtctctc
     4741 gtgcagcaat aactgttact aagccaaaat ctaaagcgac tgtacaacgt caatggttaa
     4801 atttattaag aagaagcaat aatagtggag cgatgcatat tgactgaaaa tgttattgaa
     4861 catgactgtc atggatgcaa ccagtctgtt tcttttataa aaaaacgata taaaggcaag
     4921 aaatattgtt cgacttgtta tgcccgtatc tttaaaaagc gattatgccc aagttgtggt
     4981 gactttgccc gactccctag agatgatgaa caagccattt gcaatgaatg tataaagaaa
     5041 caaccctgta ttcgatgtaa tcagacaaat aaaccaatag gtaagctgac cgaatatggt
     5101 gtggtgtgta atagttgttc cgtgtatttt agacccattg agccttgtga acgatgtggt
     5161 acgccatcac aaaaattgac ccgaatttca ccatttaatg atgatctacg ggtatgtcca
     5221 aaatgtgcga cacgagatta tgagacatgc ccatcatgcc agaaacatcg cttattggag
     5281 tctgatgtat caggacaaag aacgtgtaaa aaatgtagag ataagcctca gaaatcctgt
     5341 aaagcatgtc attgtatgat tgcagcaggt tgtgctgatt tatgcgatga ttgttattgg
     5401 catcaaaatt tatggaataa atttgatcaa aatcagaaag tatttgagtc tagtgactta
     5461 aaacagcagt atgaaaacta tataggctgg ttagaaaaga aagttggatc acataaagct
     5521 gcattatata taaataaaca tacccatttc tttattaaaa ccgaaataga ttggaatcaa
     5581 tctgtgccta ctccaaagca gttattagta aggttacgaa gtagtgggct tagaaaattt
     5641 gaattggtga tgcaatggct tgaagaggtg catgacattc gaatagatat ggataacaaa
     5701 aaaagttgtt cagaacgaga tcagatggaa aagcttgtgc aacgtatttt gcaaccatct
     5761 ttggcttatg atgttgtact ggaatacaaa aataaattag aagaaaaaat caaacgaggt
     5821 gagacttcaa tacgttcagc gaggttggct gtaaagcctg ctgtggcgtt gatgctgtca
     5881 atggagggag aaagtgctca attaccaaat ttggagcatg tcaaagcgta tcttgctgaa
     5941 tattctggtc aagctgcggc gctcactgga ttcattaact ttttaaatga gaattacggt
     6001 gcttccattg attatttaaa attaaaaaaa tctgattttt taaagactaa acaaaagaaa
     6061 aaactagaaa tggaacttat tgcacttacc caaactgatt taaatgacag tgaacttatc
     6121 ttaagttggg tcagaaatgg gcttaggtat tttcatcaac ttccttacat agatgctcta
     6181 aagattaaaa cggaaatgat tactgagatt gaggatggtt ttactgttgt gcttaatggg
     6241 caatattatt ggttgccaaa aacacaataa gttgaagcac ttaaaataat gatcagcgag
     6301 ttagctgatc attattattg agatttcagc agattatttt agtaattgtc acaccgatca
     6361 ttaatgaaat attaaaagac gcgctgaaaa ttctgagata gtagtatgtg agacctttat
     6421 agagtagatt tccatgtgtt aattaagtta tcaccaccac ctaaaaagtc acctaatgaa
     6481 gctttaaatg ctgtgtttac acaataaagt tttaaactca taaagatgaa aatcaataac
     6541 ttataacaaa aacaattgaa tagatttaca ttatagttct aaactcaatc aaacataagt
     6601 ttagaactat atgcaatttt tgacataata gatttaaact cagactaata attagcccaa
     6661 atatcattaa tcatgtattg gtttgaagtt tcaaataaaa cacaaaccaa tagcttatgt
     6721 tttgaaatct ttttagcgtc tgaaatcgct ttttgtggtt catcatatat catcgctaat
     6781 tcaggtgtcc gagcataagc aactgaaaaa gaaatattat tgtcattaat tgcagctaca
     6841 aattcatctt tttcaggtag atggataaca taacctttta ctgtactttc ttttggtaag
     6901 cctagttcag ctagaacttc tgctacactt ctttcagttc cataaaaatc atctttttgt
     6961 tcttgagctt gctttactct ttgctcaagt gtaccccttt gtttagcttg tcccatttat
     7021 tcaacctttt ggtttacaaa tatattgttt aagtttagaa cattaatgtc tagtttacga
     7081 cctgattgtc aaactacaaa tgcataacgt agaacaatca cattaaggca atcgcctatc
     7141 aattgagtag gcatatcgga tatatcatct aaattgatag atgttcttaa atgacaatca
     7201 cacaaaaaaa gtttaataat ttcagaatgt tcattatttg ctataaccat ctctgttaat
     7261 tttaaaagag aatctacgcc ttttcgataa gcttgttcat gagatacacc aaagtatgag
     7321 gatattttta acagcacatt ttcggaacaa gaaacattta gagttgggct tattaacatc
     7381 gtattcatat ttatgattcc ttttataact tattaaaaat aaagggatag atcaaaattc
     7441 taccccttca tgaaattatc gtagtaacag gactggttta ttagcgcttg tgatcacttt
     7501 tgtggtggtg ctgccaataa aaaattgacg tatctttgaa tgaccataag ccccaaccac
     7561 aatcaaatcg atttgatgct gttcagcgta attgataatt gccttatcaa cttcaccatc
     7621 caacgtattc gaggtgatat taaaaccttg tgattcaagt tgctcttttg cccaagagag
     7681 ctgattttgc atttcactat tcggatttcc gacataaacc aaatgtactg caagaccttt
     7741 aagtaatgga cttgacgcaa tgcgttcaac acattttctt gcagtctgac ttccatcaaa
     7801 agcaattaaa aaagacttag gtggactaaa tggtgcagac gttattaata cgggtttatg
     7861 aagggcacga acaacatttt caatatgggt tccaatttta ctggaatcat gctcggtttc
     7921 attcccatgc ttacccataa caagaactct aatgtcgtct tccattccca tgattgtttc
     7981 taaaacactg ccatggcgtt gaaaactttg agcatcaata gagaacttct ccaataaata
     8041 gttctttgct tcccgaagaa gaatctggcc atgctctagc tcgatttttc ccttacgttc
     8101 ttcgagttca accaattctt tgagcaaggt ttctcgactt cctagaccaa ttgcaccact
     8161 taaatcttgt ggtgcttttg gcttatcaat gacatgcaat agtttaatag gtgaattaag
     8221 tttatcgcta aaccaagctg cataatcaca cacagtattt gttactaatg atccatcaat
     8281 acatgcaata atttttgaca taactaatcc ctcttagtgg ctatttaagc gatctaaagc
     8341 atctggctta tcatggatac caaacttatc gactaatgtt ttactagctt cattcattcc
     8401 aataagttcg acttctgttc cttcacgtct aaacttaatg accactttat ctacggcagc
     8461 aaccgcagaa acatcccaga agtgtgcctg actgacatca atcaccactt ttgaaagtgc
     8521 ctctttgaaa tcaaatgcag aagtaaattt atctgctgaa ctgaagaaca cttgacctgt
     8581 taccgtataa gtacgtgtgc tttctgattc atcaaataca gattgaatcg ccatatagcg
     8641 ttcaatttta ttggcaaaaa ataatgcaga aagaagaaca ccgactaata caccataagc
     8701 caggttatgt gtggcaacaa ctacgacgac tgtaacgatc atcacgatat tgctagagag
     8761 aggatattgg cggatattgc gtagagaatc ccagttaaat gttccaattg ataccataat
     8821 catgacagca accagtgcag ccattggaat ggctttaagc cagtcactga taaacaccac
     8881 cataattaac agaaagaccc ctgcactaaa tgatgataaa cgggtacgtc caccagattt
     8941 cacattaata atggattgac caatcatggc acagcctgcc attccgccta agaagcctga
     9001 agcgacattg gcaacacctt gccctttaca ttctcggttt ttatcactgg tggtatctgt
     9061 taaatcgtcg acaattgttg cggtcatcat ggactcaagt aaacccacgg ctgccaaacc
     9121 tagtgaatag ggtaaaataa tagctaaagt ttcaagattt aaaggaatat caggaagcaa
     9181 gaaaattgga agcgtatccg gcaattgccc catatcaccg acagttctta catctaaacc
     9241 aatgaataag gcaaagagcg taagtaggac aatacagatg aggggagagg gtaaaagttt
     9301 ccctatcact gggatatagg gaaatagata aataaccgct aaaccaccga ttgtcatcgc
     9361 ataaacatgc caagtgacgt tggtaagttc aggcaattga gccataaaaa ttagaatagc
     9421 caaggcattt acaaagccaa taacgactga ctttgataca aaacgcatca aggctcctaa
     9481 ttttagatag cctgctagga tttgaataaa tcctgtcaaa attgtggctg ctaataaata
     9541 ttgcagacca tgctctttaa ccagtgtgac cattaataaa gccatagcac cagttgcagc
     9601 agaaatcata ccaggacgac cgcccacaaa agcaatcaca acagcaatac agaatgaagc
     9661 atatagcccc actttaggat caacgcctgc aataatggag aaagcaattg cttcaggaat
     9721 tagagccaaa gcaacaacaa tacccgcaag aacatccccg cgaatctctg tacacgacaa
     9781 atttcacaga acccttatcc tatcaggatt ctgccttctt aaaattgcca aaatttcctt
     9841 aaactcttct tttttcccaa aaccaattaa acgctgaatc gccatttgaa catagtctaa
     9901 accatagcga aataaactca ttgagagtcg tccatgcttc tttattttta tcgctttttt
     9961 ttgatcatgt tgccattcac ccgttaagta acaccaacag aagcttatag ctaacaccgc
    10021 aatcaatttt ttcactcgtc tagggtctgt caagcgcgta ttttcaagat taaacscgcg
    10081 tcctttgaga caactgaata aggtttcaat ttcccagcgt aatgcataat cctgaatagc
    10141 attggcatta aactgaggag aaacgacgag taaaagctct ccattttcta actgtagtgc
    10201 acttatatat agtttcaccc gaccaaccaa aatccgtcgt ttacgacatt caatttgacc
    10261 aactttaaga tggcgaaata aatcactaat tttatgattc tttcctaaat gattggtgac
    10321 aatgaagttt ttttaacacg aatgcagaag ttgatgtctt gttcaattaa ccatgtaaac
    10381 cactgctcac cgataaactc tctgtctgcg aacacattca caatacggtc tttaccaaaa
    10441 atggctataa agcgttgaat caaagcaatg cgctctttcg tatctgaatt tccacgttta
    10501 ttaagcaatg tccaaaggat aggtatcgct attccacgat aaacgattgc gagcatcagg
    10561 atattaatat ttcgttttcc ccatttccaa ttggttctat ctaaagtcag ttgcacttgg
    10621 tcgaatgaaa acatattgaa aatcaactga gaaatttgac gataatcaaa atactgacct
    10681 gcaaagaagc gctgcatacg tcgataaaat gattgtggta agcacttgat gggcaaggct
    10741 ttagatgcag aagaaagatt acatgtttgc tttaaaataa tcacaagcat gatgagcgca
    10801 aagcacttta aatgtgactt gttccatttt agagatttgt ttaagataag atataactca
    10861 ttgagatgtg tcatagtatt cgtcgttaga aaacaattat tatgacatta tttcaatgag
    10921 ttatctattt ttgtcgtgta cagaggtatt tgcattgctt caatcgaaaa tgcacatggt
    10981 ttgggttaaa actattgcag gaaagttaga ggaacgtatt cgttacactt cagcaatttg
    11041 ctataacact tttcctgtgc caaagctcat gaaagcatcg atatttaagt taaatgaatc
    11101 tgcttttaaa atactagctg tacgtgaatc ctattcacat ttaagtttgg ctcaacttta
    11161 tgatcctgaa aaaatgcctt ttgacttaaa acaagctcat aaagagaatg attcgttagt
    11221 ggaaaaacta tataaatcaa gtgattttaa gacagatgaa gagcgtctag agcgattatt
    11281 ccactactac gaaacaatgt taaattgaga taaaaatgaa aaatttagta gaagtaaatt
    11341 atcaacaaac tggagaaagc acctcagtta ataatatggg gatgcgagct atgcaagctc
    11401 gcgccttcga atctcgtaat agtcagtatt tgctcttaaa agcccctcct gcatcaggta
    11461 aatctcgtgc attaatgttt ttgggtttag acaagataca caaccaaggg ttaagcaaag
    11521 ttattgtagc tgtacctgaa cgctcaattg gcggttcatt tgctagcaca aaactaagcg
    11581 aaagtggctt ttttgcagat tgggatatca aacctgaaaa caacttatgt actgctggtg
    11641 gcgataaaag caaagtaaaa gcctttaaac actttatgga aggtacagat caaattttag
    11701 tatgtacaca tgccacacta agatttgctt ttgaagagct tgaagatcat gtatttaata
    11761 atacattagt caaaatacac gacaattttt gcagaatctg ccagccaatg atgttctcct
    11821 tactggttca cgcggcacag gaaaatcttc aattgtccgt gcccttttaa ccgagtataa
    11881 agatcaagga ttacgtttaa ttgaaattga acgtgatgat ctgtccgatc ttcctgaaat
    11941 ccagaagctc atccagaagc gtccggaaaa gttcattgtc tactgcgatg atctggcctt
    12001 taatgctgaa gatgaaaact atcgcagctt aaaaagtgta cttgatggct cattacagtc
    12061 aggtacaagt aattttatta tttatgcaac cagcaaccgt cgacatctgt taccagaatt
    12121 tatgcatgag aacacgccag tgactcgtgt agatgtaccg caatataccg agttgcatcc
    12181 acaagaagcc attgaggaaa aaatttcact ctcggatcgt tttggaatgt ggctttcatt
    12241 ctatccaatg gatcaaaacc tgtatttaac cattgtagaa cactatcttg ctaaaacaga
    12301 tatgccaatg aatgacgaag cccatgctga agccctacgc tggtgtcagg cacgtggtca
    12361 acggtcagga cgtgccgcct atcagttctc taaacactgg attggatcac aacaactaaa
    12421 agcactgtaa aattttaaac tagaaaaatt aacaggtttc attattagtc agattataaa
    12481 aggcccattt atctcaaatg ggctttttat aaaattagag tttctgtcaa gctcttaaat
    12541 aatattcagc tgttttaatg atttcatcaa taattcatta cgtatagatg ccggcatttc
    12601 tgaccgcatt tccatattta atgcaaaagc gacaattttt ccatctggct gctcaaccca
    12661 gccggtcaac cagcccactt gtggttttat atccattgcc caaccagtct ttccaaaaat
    12721 tttgtagcca ttactctctt ctaaaagaag catatttttt acattagcct gcactttttc
    12781 actaaatgga agctgtgtat gtgctaattg ggaaacaaac tctacctctt gaataggcgt
    12841 aacctttaat ggtcctacca accagaaatt atcaacctgc tgtccaattt cagcattacc
    12901 gaaaccaata cgttttactt ctttttgcat gagatcaaga ccgatacgtc gcgcaagttc
    12961 ctgatagact gggactgcag aaagcttcat ggcttctcct agtgtcatgt ctttttccca
    13021 agcggtaaat gaccttttct cgcccttcca tttaaatatt tcattaatat ccgttttctg
    13081 gttctccaat ccgatcaggg cattcaacat tttaaatgta gaggctggca catattctgt
    13141 atttgcgcgg cttagagcat taccatatag attaattttt ttatctgttt gaataaccag
    13201 cacacctgag gtgttttttt catcaaagta ttgatgaatc acctgattat gtccttgaac
    13261 aatctgactc ggggtttcat ttattaaatt atgctgaacc gtacaaccag aaagaaaaag
    13321 agaagcaacc acatagcaag taaaatattt attcatttta aacaccagat caatagaaaa
    13381 taaaaaagag ctctgtacac gacaaaaata gataactcat tgaaataatg tcataataat
    13441 tgttttctaa cgacgaatac tatgacacat ctcaatgagt tatatcttat cttaaacaaa
    13501 tctctaaaat ggaacaagtc acatttaaag tgctttgcgc tcatcatgct tgtgattatt
    13561 ttaaagcaaa catgtaatct ttcttctgca tctaaagcct tgcccatcaa gtgcttacca
    13621 caatcatttt atcgacgtat gcagcgcttc tttgcaggtc agtattttga ttatcgtcaa
    13681 atttctcagt tgattttcaa tatgttttca ttcgaccaag tgcaactgac tttagataga
    13741 accaattgga aatggggaaa acgaaatatt aatatcctga tgctcgcaat cgtttatcgt
    13801 ggaatagcga tacctatcct ttggacattg cttaataaac gtggaaattc agatacgaaa
    13861 gagcgcattg ctttgattca acgctttata gccatttttg gtaaagaccg tattgtgaat
    13921 gtgttcgcag acagagagtt tatcggtgag cagtggttta catggttaat tgaacaagac
    13981 atcaacttct gcattcgtgt taaaaaaact tcattgtcac caatcattta ggaaagaatc
    14041 ataaaattag tgatttattt cgccatctta aagttggtca aattgaatgt cgtaaacgac
    14101 ggattttggt tggtcgggtg aaactatata taagtgcact acagttagaa aatggagagc
    14161 ttttactcgt cgtttctcct cagtttaatg ccaatgctat tcaggattat gcattacgct
    14221 gggaaattga aaccttattc agttgtctca aaggacgcgg gtttaatctt ggaaatacgc
    14281 gcttgacaga ccctagacga gtgaaaaaat tgattgcggt gttagctata agcttctgtt
    14341 ggtgttactt aacgggtgaa tggcaacatg atcaaaaaaa agcgataaaa ataaagaagc
    14401 atggacgact ctcaatgagt ttatttcgct atggtttaga ctatgttcaa atggcgattc
    14461 agcgtttaat tggttttggg aaaaaagaag agtttaagga aattttggca attttaagaa
    14521 ggcagaatcc tgataggata agggttctgt gaaatttgtc gtgtacagag cccgcgaata
    14581 ttggagaacc attgttctcg aatagtggat aacatgaaaa tagcctattt ttttaaattt
    14641 taggaaaggc tagtgatgtg atatatacaa cacacaagcg actacaccac atcactagac
    14701 agtgatgagt tatttacaaa aagatgggta aataaactag ggtggagtca aaagcataag
    14761 tgtgtgaata cgaatagaaa tttaaattga actgttagta taggcgaact gaacgaaaat
    14821 ttcactcttt tttatttcgc aaatttatag aaaacatgaa ataccaagta atttcatgaa
    14881 catatgattg tataaaaaat attctttaat taggatttgt ccttctgtaa gcttatgaat
    14941 aaatttttaa agcgattttt ccattcatct ggtgttgcgt ataaatgttt tattgcaatc
    15001 attttatgaa tgaaactatc acagtagagt gcccattttt ttattccctt ggcattttct
    15061 agttcaatac agattttttt aatttgttcg cttgttaact tattctgagt aaaaataaga
    15121 aaggcacaaa atgcttcatg atttttattt ttatgtatat cgatgagata ggaaaatcca
    15181 ttttgatctt ttaagtctaa ttttaagaac aataattttc gccctttatc ataatcaatc
    15241 catgagggat gcttgggttc aggcacaagt tttttgattt gaagcaaatt aatactcgct
    15301 atcatttcac catgttcatt aattgcagac agatgttcca gaggagaacc catttcagaa
    15361 gcaagttctt ggagagattg gataatgtat tgaatgtcaa aacgttcatc tctgctaggt
    15421 ggtgtatctg tcgtttcaga ttttttgatc ttagtatttt gattaccata gggttcatca
    15481 gaagatgttt caacctcttc tgatttatct gaataaggtt gattagatgc tccgacttca
    15541 attggatcat cggttggttc attaaaagaa tacttcagaa taccttgtgt atttccagtt
    15601 tccacctcag aacgcttacg atattcaccg cttgttcttg atggtggttt ttgagtattg
    15661 atgtgctcat tcttattttg acttttttca cgaggtctag gatttttctc atcaggcttt
    15721 gaaattgtat tggtcaggtc tttgttgaca tcaatcatgt gatcgttaat aggatcaaat
    15781 tttttcactc tagtgataaa aaaacgagtc ttattgtcat ctagccaaat tccttctgct
    15841 tcaaggaaaa gctttgtggg atgtggtgga aacacaatgg gatacctttt gcctgtaccc
    15901 tgctgaagat gattaaactc ggtaatttcc atacttcgtt gaattttatc aactatcgtt
    15961 tgcacatatg ggtttaaggc aagattagca agaaaaataa ttgcagtctc gcctatatct
    16021 tcatatggct ttctgatttt aattttatat tcaacattat cgtccacaat ttctgttgta
    16081 taagattcta aaaaacgatt aatgatggat tgactcgaag ttcctaataa aagtccccta
    16141 atatccttcc gattgacgaa taagctatgc aatacatgaa ttgatgatgt gatgacttga
    16201 gttccatcat tggaatgcat taggcaatga ttcactttgc gatatgaatc attggtatat
    16261 ttggtaaatg gatagccttg aagaacataa tcaactttat ttgggaggaa atagtttttt
    16321 aaaggaatgt agtagtcaga agtattgggg attctgtccg taagttttat attcttaggt
    16381 ttattattgg tgaagtcgaa agaaaatcgt tttgtaatga gttttcctga aaaccgatag
    16441 gattgatcgg ttaaaacatt tccatcccaa atactgccat gttgaacagc atcaagatca
    16501 agtacgggaa caaggaatgt tgagcactta tctaaaaata accattgatc actttttcta
    16561 atctcaatta ataacacttc aacaaaagga ttagttgaat cgttatcttc gtttctaaca
    16621 agtccaccat atctataaac cactcgacta atattatcgt ttgggaagca atcaatagtc
    16681 gttattttgt cattattaac aggcatatat ttttacaaaa tgaaaaatta aagcatatag
    16741 ataatatact ttttattctt gctctagttt atttgtgtaa actctgattt atttgtggaa
    16801 ctgaca
//
