LOCUS       Tn6427                  6956 bp    DNA     linear       29-SEP-2020
DEFINITION  Pseudomonas aeruginosa PA96.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa PA96
  ORGANISM  Pseudomonas aeruginosa PA96
            Unclassified.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn6427
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Pseudomonas aeruginosa PA96"
                     /mol_type="genomic DNA"
     repeat_region   1..38
                     /gene="IRL_Tn6427"
     CDS             complement(34..2844)
                     /gene="tnpA"
                     /codon_start=1
                     /product="Tn6427 transposae"
                     /translation="-
                     QLCLLRFPGYALGTDSERPEPVILWVAKQVQTDPASWEKYGERDVTRREHAQELRTYL
                     QLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQRRRILPALSVIDRACSEA
                     IARANRRVYRALVDPLTDSHRAKLDELLTLKAGSSITWLTWLRQAPLKPNSRHMLEHI
                     ERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYATLAAVVLES
                     TATVIDELVDLHDRILVKLFSSAKHKHQQQFQKQGKAINDKVRLYSKIGQALLEAKES
                     GSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRYTPALLEVLE
                     LRAAPAAQGVLAAVQTLREMNADSLRKVAADAPTAFIKPRWKPLVITLEGNDRRFYEI
                     CALSELKNALRSGDIWVKGSRQFRDFDDFLLPAEKFATLKREQALPLTINPNSDQYLE
                     ERLPLLDEQLATVIRLAKDNEMPEAILTESGLKITPLDSAVPDRAQALIDQTSQLLPR
                     IKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESSPGL
                     TYAKLSWLQAWHIRDETYWAALAELVNHQFRHTFAAHWGDGTTSSSDGQRFRAGGRGE
                     STRHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIEE
                     HYTDTAGFTHHVFALMHLLGFRFAPRIRDLGETKLYVPQGVHAYPALRPLIGGTLNIK
                     HVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFTLDW
                     LQSVELRRRVHAGLNKGEARNLLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIV
                     LWNTVYLERATQGLVEAGKPVDGELLQYLSPLGWEHINLTCDYGWRQSRRLEDGKFRP
                     LRLPGKP"
     CDS             complement(3005..3577)
                     /gene="tnpR"
                     /codon_start=1
                     /product="Tn6427 resolvase"
                     /translation="MTSACQSHRFQPDKFSSYDQNPERQLEQTEVSKLFTDKASGKDN
                     QRPQLEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTG
                     EDSPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQ
                     RATAGEPKAQLAREFNISRETLYQYLRTDD"
     misc_recomb     3534..3660
                     /gene="res"
     CDS             3803..5461
                     /gene="mcp"
                     /codon_start=1
                     /product="Methyl-accepting chemotaxis protein"
                     /translation="MSGWLARFSIKTRLIGLLVLLASMAVGLPMAGLNGMRTSNAVID
                     RLYNNMLLPIDKLGDINNHMHNARAQLLLALQHEPGSAFEKAHDHPSSMHFDRVDKSV
                     SIIRERLSDYQRIPMSKEQQRLSTELQNKLSDYLDNGVLPTLNAMRQGDYAGGNQILL
                     TRLDPAFMTTYASLRTLIESEASAAELSFQNADKQFERLVAMIGLALVVALSLVTVTA
                     LATVPSITKGVRELQVGGQQLADGNLGYRVTYQGRDELGEISSAFNEMASRFHHTFQE
                     LAGAVEQLAAAAEQTAQASSQTSDGIRRQQLETDLIATAMNEMSATVQDVAGNAASAA
                     RAAQEADQQSESGMRVVHQTIASIDSLASEVEHAATVIHELEADSAGISSVVDVIRSI
                     TEQTNLLALNAAIEAAHAGEHGRGFAVVADEVRSLASRTQQSANEIQAMIEKLQSGAN
                     RAVAVMESSCSKAQAGKQQVASAGHMLEQISNAVATINDMNAMIASAAEEQSSVAEEI
                     SRNVTNVSQIAEKTSEASRQNVATSTQLASLASQLQRLMHMFRL"
     repeat_region   5564..5590
                     /gene="IRL_ISPa73"
     CDS             5629..6879
                     /gene="tnpA"
                     /codon_start=1
                     /product="ISPa73 transposase"
                     /translation="MTVVKRTKRAKPDPELVKLADSLLVNYKKPEDLIGENGLLKPAH
                     QDAGRACAGSRDDRAPGHGKSGSIFNAGGNARNGHSDKTLKGDFGELPLAIPRDRQAL
                     FEPQLVAKQQTRWTGFDDKIISLYARGLSVREIQGHLEEMYGTEVSPTLISTVTDAVS
                     DEVKLWQGRPLDPLYPILYLDCIHVKVRDAGAVRTKAVYLAIGVNMDGHKEVLGLWIA
                     QTEGAKFWLQVVTELKNRGVQDIFIACVDGLKGFPEAIETVYPKACVQLCIVHMVRNS
                     LNFVSWKARKEVAADLKLVYTSATVELAEQRLTEFEEKWGGQYQSIGLSWRRNWARVI
                     PFFDHPAEIRKVIYTTNAIESINMSLRKVIKTRASFPTDEAVSKLFYLALNNISKKWT
                     MPIRDWKAALNRFSIQFEDRLMQD"
     repeat_region   6889..6915
                     /gene="IRR_ISPa73"
     repeat_region   6919..6956
                     /gene="IRR_Tn6427"
ORIGIN      
        1 ggggagcccg cagaattcgg aaaaaatcgt acgctaaggt tttccaggca accgtagcgg
       61 cctgaacttc ccgtcttcca gcctgcggct ctgccgccag ccgtaatcgc aggtcaggtt
      121 gatgtgctcc cagcccagcg gcgacaggta ttgcagcagc tcgccgtcca ccggcttgcc
      181 ggcttcgacc agtccctggg tggcgcgctc caggtacacc gtgttccaca gcacgatggc
      241 ggccgtcacc aggttgaggc cgctggcccg gtagcgctgc tgctcgaagc tccgatccct
      301 gatttcccca aggcggttga agaacaccgc cctagccagc aagttgcgag cctcaccttt
      361 gttcaggccg gcatgcacgc ggcggcgcag ctcaacactt tgcaaccagt ccagggtgaa
      421 cagcgtgcgt tcgatccggc ccagttcgcg cagggcgacg gccaggccat tctggcgcgg
      481 atagctgccg agcttgcgca gcatcagcga ggcggtgacg gtgccctgct tgatcgagct
      541 ggccaggcgc aggatgtcat cccaatgggc acggacgtgc ttgatgttca gggtgcctcc
      601 tatcagcggg cgcagcgccg ggtaggcatg cacgccctgc ggcacataca gtttggtttc
      661 gccgaggtcg cggatgcgtg gtgcgaagcg gaagcccagc aggtgcatta gggcgaagac
      721 gtgatgggtg aagccggccg tgtcggtgta gtgctcctcg atccgcaagt cggactcgtg
      781 gtacagcagg ccatcgagca cgtaggtgga atcgcgcacg ccgacattca ccacgcgggt
      841 gctgaatggc gcgtactggt cggagatgtg ggtgtagaac agccggcccg gctcgctgcc
      901 gtacttcggg ttgacgtgcc gggtgctttc gccccggccg cccgcccgga agcgctggcc
      961 atcggacgaa gaggtcgtgc cgtcgcccca gtgagcggcg aaggtatgac ggaactggtg
     1021 gttgaccagc tcggccaggg ccgcccagta ggtttcgtct cggatgtgcc aggcttgcag
     1081 ccaggacagc ttggcgtagg tcaggccggg gctcgactcg gccatcttgg tcagcccgag
     1141 gttgattgca tcaccgagga tcgcggacag cagcaacgtc cggtctttgg cctcggcccc
     1201 gtccttcaag tgggtaaagt ggcggctgaa gcccgtccag tcgtcaacgt ccatcagcag
     1261 ttcggtgatc ttgatgcgcg gcagtaactg gctggtctgg tcgatcagcg cctgcgcacg
     1321 atctggcacc gctgaatcca gcggggtgat cttcagcccg gactcggtga ggatggcctc
     1381 gggcatctcg ttgtccttgg ccaggcggat gacggtggcc aactgctcgt ccagcagcgg
     1441 caatcgctct tccagatact ggtcgctgtt gggattgatc gtcaggggca gggcctgttc
     1501 ccgcttgagc gtggcgaact tctctgccgg cagcaggaag tcgtcgaagt cacggaactg
     1561 ccgcgagccc ttgacccaga tgtcgccgga gcgcagggcg ttcttcagct cggaaagcgc
     1621 gcagatttca tagaagcgcc ggtcgttgcc ttccagggtg atcaccagcg gcttccagcg
     1681 gggcttgatg aaggccgttg gtgcatcggc cgccaccttg cgcaggctgt cggcgttcat
     1741 ctcgcgcagg gtctgcacgg ctgccagcac gccttgcgcg gccggcgcag cgcgcagttc
     1801 cagcacctcc agcaaggccg gcgtgtaacg gcgcagagtg gcgaagttct cgccgaccag
     1861 gtgcagatgg tcaaagcctt ccggccgggc aagcagctcg gcctcgctga cgctctcggt
     1921 gaactcgtcc caaggtatca ccgcttcgat ggctgcatag gggtcgctgc cgctttcctt
     1981 ggcctccagc agcgcctggc cgatcttcga atacaggcgc accttgtcgt tgatcgcctt
     2041 gccctgtttc tggaactgct gctgatgctt gtgcttcgcg ctgctgaaca gcttgaccag
     2101 gatacggtcg tgcagatcga ccaactcatc aatcacagtc gcggtgctct ccagcaccac
     2161 cgcggccagg gtcgcgtagc gtcgctgagg ctcgaacttg ccgaggtcct tgggtgtcat
     2221 ctgcccaccc tcgcgggcca gcttgagcag gcggttctga tggatgtgcc ggcccaggcc
     2281 ttcgggcaag tccaccagct gaaatgtctt cagccgctcg atgtgttcaa gcatgtgccg
     2341 ggagttgggt ttcagcggtg cctgtcgcag ccaggtcaac caggtgatgc tgctgccggc
     2401 cttgagcgtc aacagctcgt ccagcttggc ccgatgcgag tccgtaagtg gatcgaccag
     2461 ggcgcggtag acccgccgat tggcccgcgc aatggcctcc gagcaagccc ggtcgatcac
     2521 gctcaacgcc ggcaggatgc gtcgcctctg ccgcaggctc tccagcgcct gaccggccag
     2581 cagcaagccc ttgtcggtct gctgggccag ttcggtcagc tcgcgcacca gggcgcggaa
     2641 gtcggacagg ccgaacgggg ccagttgcag gtaggtgcgc agttcctggg cgtgctcgcg
     2701 acgggtcacg tcgcgctcgc cgtacttctc ccaactcgcc ggatcggtct ggacttgctt
     2761 ggccacccac aggatcaccg gctcgggccg ctcgctgtcg gtgcccaacg catagccggg
     2821 gaagcgcagc aggcagagtt gcaccgcaaa gcccagacgg ttggcgtcgc cagcgccgct
     2881 ggcggatcag cgacaggtcg gaatcgttga aggtgtagta gcggatcagg tcatcctggc
     2941 tttccggcag cgcgagcagg gtgtcccgct ccgtggccga gaggatcaag cgacgcggca
     3001 tgtatcagtc gtccgtgcgg aggtactggt agagggtttc ccggctgatg ttgaattcac
     3061 gggcgagctg cgccttgggc tcgccggccg tcgctcgctg ccgcagggta gcagcctgct
     3121 catcggacag ggctttcttg cggcctcggt acgcgccgcg ctgcttggcc aaggcgatgc
     3181 cctcgcgctg ccgctcacgg atcaaggcgc gctcgaactc agcgaaggcc cccatcacag
     3241 atagcatcag gttggccatc ggggagtcct cgcccgtgaa caccaagccc tccttcagga
     3301 actcgatgcg cacgccgcgc tgggtcagct tctgcaccag gcggcgcagg tcatcgaggt
     3361 tgcgcgccag acgatccatg ctatgcacca ccacggtgtc gccctcacgg acgaagctca
     3421 gcaacgcctc cagttgcggg cgctgattat ccttgcccga ggccttgtcg gtgaacagct
     3481 tgctcacctc ggtctgttcc agttggcgtt cagggttctg gtcgtaactg ctgaatttgt
     3541 caggctgaaa tctatgactt tggcaagcag aggtcaaaaa attaatgatg gactctatct
     3601 gacggcgtag cgctgggctg cctgacatcc ggttagggta tagcccaacc tgacggcctt
     3661 taggtcttgt atttgatttg agtactaata tttcccccgg ccatatggcg agaagccttg
     3721 agggggagcc tgactcaaga tttcaaatga accagccgat acaatgaagg cacattgaaa
     3781 tatctaataa tgaggtgaca cgatgagtgg ttggctagcc cgtttctcaa taaaaacccg
     3841 tctcatcggc ttgctcgtgc ttctagcttc tatggctgtc ggcttgccaa tggctggtct
     3901 taatggaatg cgcaccagca atgcagtgat tgatcggctg tacaacaaca tgttgctacc
     3961 gatcgacaag cttggcgaca tcaataacca catgcacaac gcgcgtgctc agcttttgct
     4021 tgcccttcag catgagcccg gaagtgcctt cgagaaggcg catgatcatc catcgagtat
     4081 gcatttcgat agagtggata aaagcgtaag catcatccgt gagcgccttt ctgactacca
     4141 gcgtatacca atgagcaaag aacagcagcg ccttagcact gagctgcaga acaaattgtc
     4201 tgattacttg gataatggtg ttctgccgac gctcaatgcg atgcgccaag gcgactatgc
     4261 gggagggaac caaatcctgc taacccgcct tgatcctgct tttatgacta cctatgccag
     4321 cttgcgcact cttattgagt cggaggcgtc ggcagctgaa ttaagctttc aaaacgcgga
     4381 taagcaattc gagcgtcttg tggcgatgat aggactggcg ctggtagtgg ctctgtctct
     4441 ggtcacagtc acggctctgg ccactgtgcc aagtatcacc aaaggcgttc gggagcttca
     4501 agtcggcggt cagcagctcg ctgatggcaa tttgggctat cgagttacct atcaggggcg
     4561 tgatgagctg ggcgaaatat ctagcgcatt caatgaaatg gcttcgcgtt ttcaccacac
     4621 ctttcaagag ttggcaggtg ccgtggagca actggcagct gcagctgagc agacggccca
     4681 ggcaagtagt caaaccagtg acggtatccg ccgccagcag ctggagacag acctgatagc
     4741 tactgcgatg aacgaaatgt ccgctactgt tcaggacgta gcggggaacg ctgccagtgc
     4801 ggcacgggcg gcacaggagg ccgatcagca gtctgaaagc ggcatgcgcg tggttcatca
     4861 aacaatcgct tctattgaca gcctcgccag tgaagttgaa cacgctgcca ctgtgattca
     4921 tgagcttgag gcagacagtg ctggtattag ctctgtagtg gatgtcattc gaagcattac
     4981 cgaacaaacc aacctgcttg cccttaacgc ggctattgaa gctgcacatg ctggtgagca
     5041 cggacgaggc tttgctgttg tcgcggatga ggtgcgctcg cttgcttcgc gcacgcaaca
     5101 gtcagccaac gaaattcagg ccatgattga aaagctgcag agcggcgcaa atcgtgctgt
     5161 tgctgtcatg gaaagcagtt gcagcaaagc acaagctggt aagcagcagg ttgccagtgc
     5221 aggccatatg ctggagcaaa tcagtaatgc tgtggcgacc attaatgaca tgaatgccat
     5281 gattgcttct gcagctgagg agcaaagctc tgtcgcggag gagatcagcc gtaacgttac
     5341 caacgtaagc cagattgccg aaaaaactag cgaagcctca cgtcagaatg ttgccaccag
     5401 tacgcaattg gcttctctgg caagccaact tcagcgtctg atgcacatgt tccgtcttta
     5461 gggaaactct gaaaagcccc agcttcgcgg aattgatgcg ctgtaacccg ctcggctgct
     5521 gggctctttt tcccgagaaa ggacttcctc aggccatcct gaggagggtg tttttaactt
     5581 tgtgtaaacg gtcatttggc aggagactgc cagcctcaag gagtacccat gaccgtagta
     5641 aaacgcacca agcgagcgaa gcccgaccct gaactcgtta agctcgccga cagcctgctg
     5701 gtcaattaca agaaaccgga agatttgatt ggcgaaaacg gcctgctcaa gccagctcac
     5761 caagatgctg gtcgagcgtg cgctggaagc cgagatgacc gagcacctgg gcatggcaag
     5821 agtggatcga tcttcaacgc cggcggtaat gcccgtaacg gtcacagcga caaaaccctc
     5881 aagggcgatt tcggcgaact gccgctggcc atccctcgtg accgccaggc gctgttcgag
     5941 ccgcagctgg tggccaagca gcaaacccgc tggacgggct tcgacgacaa gatcatctcg
     6001 ctgtacgccc gcggcctgag cgtacgagag atccaggggc acctggagga gatgtacggc
     6061 accgaggtct ccccaaccct catctcgacc gtcacggacg cggtaagcga cgaggtgaag
     6121 ctctggcagg gccgtccact cgatccgctg tacccgatcc tttatctgga ctgcatccac
     6181 gtcaaagtgc gcgatgccgg cgcggtgcgg accaaggcgg tgtacctggc tatcggggtc
     6241 aatatggacg ggcacaagga agtgctgggg ctgtggatcg cccaaaccga gggtgccaag
     6301 ttctggttgc aggtagtcac cgagctgaag aaccgtggcg tgcaggacat ttttatcgcc
     6361 tgcgtggacg gcctcaaggg cttccccgag gcgatcgaaa cggtctatcc gaaagcctgc
     6421 gtgcagttgt gtatcgtgca catggtgcgc aacagcctga acttcgtgtc ctggaaagcg
     6481 cgcaaggaag tggccgccga tctgaagctg gtctacacct cggcgacggt cgaactggcc
     6541 gagcaacggc tgacggagtt tgaagaaaag tggggtggtc agtaccagtc aatcggcctg
     6601 tcctggcggc gtaactgggc acgggttatc ccgttcttcg accatccggc cgagatccgc
     6661 aaggtgatct acaccaccaa cgccatcgaa tcgatcaaca tgagtttgcg caaggtgatc
     6721 aagacccggg cctcgttccc aactgacgag gccgtgagca agctgtttta tctggcgctg
     6781 aacaacatca gcaagaagtg gacgatgccg atacgggact ggaaagcggc tctgaatcgc
     6841 ttcagtatcc agttcgaaga tcgattgatg caggattaat agaaatcccg tttacacata
     6901 atccgggaca ccctcatcct tagcgtacgt tattttccgg attctgcggg ctcccc
//
