LOCUS       Tn6394                  7240 bp    DNA     linear       29-SEP-2020
DEFINITION  Pseudomonas aeruginosa.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Unclassified.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn6394
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7240)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
     repeat_region   complement(1..25)
                     /gene="IRR_ISPa17"
     CDS             complement(175..717)
                     /gene="tnpA"
                     /codon_start=1
                     /product="ISPa17 transposase"
                     /translation="MSMNEFRRLAAKIDQHMQQLAAQGVSEAHAIINRMMGYGPDLHR
                     IWVGTSDQQLMALSREFPGFYRYARIMEEASEAERRKASRPYDGMAEFSEQHKQMGAQ
                     LLTTAATLERGYQAFRASGSLQDFRPQLDELGRLHRQWLSDLEAFKDSLRTQGAEPKV
                     LEYVNEAFGRLAERIKQLAG"
     CDS             complement(723..1118)
                     /codon_start=1
                     /product="Toxin/antitoxin system toxin component, PIN
                     family"
                     /translation="MKVAVDTNVLVRAVVRDDPAQADVAAAVLTDAELIAVALPCLCE
                     FVWVLLRVYGFQQADAASAIRALLAAANVEVNRPAVEAGLLVLDAGGDFADGVIAYEG
                     NWLGGETFVSFDKKAVALLTAQGQSTRLL"
     CDS             complement(1115..1366)
                     /gene="mazE"
                     /codon_start=1
                     /product="Toxin/antitoxin system antitoxin protein MazE"
                     /translation="MTTLTVTARGQVTFRKDVLQHLGIRPGDKIELDLLPDGRGVLKA
                     ARPAGTIASFVGLLAGKTQKVATIEEINEAAAQGWAGKQ"
     CDS             1431..2114
                     /codon_start=1
                     /product="Putative recombinase"
                     /translation="MSVKRSLVGHAGHCPAKLLHNGLSDIKIGGRNDGQERRQMALIG
                     YARVSTAEQDTALQTDALRKAGCERVFEDTASGAKADRPGLADALAYLRDGDVLAVWR
                     LDRLGRSMPHLIETIGALEARGVGFRSLTEAIDTTTPGGRLIFHVFGALGQFERDLIR
                     ERTKAGLTAAAARGRKGGRKPVVTADKLQRAREHIANGLNVREAATRLKVSKTALYTA
                     LQSTSAADS"
     repeat_region   complement(2121..2145)
                     /gene="IRL_ISPa17"
     repeat_region   2259..2283
                     /gene="IRi_In992"
     repeat_region   2354..2378
                     /gene="IRL_ISCfr1"
     CDS             2435..3805
                     /gene="tnpA"
                     /codon_start=1
                     /product="ISCfr1 transposase"
                     /translation="MMGQLPGGQQRLFYSFNLEDHVPAQHLLRSIDQCLDLSDLRAYL
                     ADFYSPIGRPSIDPELMVRMLVVGYCYGIRSERRLCEEVHLNLAYRWFCRLGLEDEVP
                     NHSTFSKNRHGRFRDSDLFRWLFNEVLRRCMAAGLVKGEGFAVDASIIKADASRQRGV
                     AGDEVDWNDPKLSSRAVREYLEALDEEALAEALPKKISLTDPQSRWTAAPGGPAFFAY
                     STNYLIDTEHGVIMDVEATPAHRTAEVDSTRTMVERVKAQFDLTPERLIGDTAYGTAP
                     MLAWMVEEKDIEPHVPVWDKTERKDDSLSSNDFHWNQEANEYRCPAGKPLRSEWRAFT
                     QKRSRVTKAHTIIYRSSQTDCTTCPLKAKCCPNTPNRKIVRSIHEAARDVARRIAKTP
                     EYLVSRCERKKVEMLFAHLKRIMKLDRLRLRGLTGATDEFTLAAAVQNLRRMAKLLPQ
                     GPPLTG"
     repeat_region   3946..3970
                     /gene="IRR_ISCfr1"
     CDS             complement(4072..5085)
                     /gene="intI1"
                     /codon_start=1
                     /product="IntI1 integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     misc_recomb     5166..5228
                     /gene="attI1"
     CDS             5232..5690
                     /gene="aacA7"
                     /codon_start=1
                     /product="Aminoglycoside 6'-N-acetyltransferase"
                     /translation="MDSSPLVRPVETTDSASWLSMRCELWPDGTCQEHQSEIAEFLSG
                     KVARPAAVLIAVAPDGEALGFAELSIRPYAEECYSGNVAFLEGWYVVPSARRQGVGVA
                     LVKAAEHWARGRGCTEFASDTQLTNSASTSAHLAAGFTEVAQVRCFRKPL"
     misc_recomb     5707..5818
                     /gene="attC_aacA7"
     CDS             5829..6569
                     /gene="blaIMP-1"
                     /codon_start=1
                     /product="Metallo-beta-lactamase IMP-1"
                     /translation="MSKLSVFFIFLFCSIATAAESLPDLKIEKLDEGVYVHTSFEEVN
                     GWGVVPKHGLVVLVNAEAYLIDTPFTAKDTEKLVTWFVERGYKIKGSISSHFHSDSTG
                     GIEWLNSRSIPTYASELTNELLKKDGKVQATNSFSGVNYWLVKNKIEVFYPGPGHTPD
                     NVVVWLPERKILFGGCFIKPYGLGNLGDANIEAWPKSAKLLKSKYGKAKLVVPSHSEV
                     GDASLLKLTLEQAVKGLNESKKPSKPSN"
     misc_recomb     6572..6698
                     /gene="attC_blaIMP-1"
     misc_feature    complement(6668..7099)
                     /gene="truncated tniA"
                     /product="Truncated DD(35)E transposase TniA (pseudogene)"
     repeat_region   7216..7240
                     /gene="IRt_In992"
ORIGIN      
        1 tgtcgttttc agaaggcgac tgcaccagtt cactgggctg gccgccgtgt gtgcatagaa
       61 cttctgaccg ggaacggtca gaagttctat gcacgaagcg ccgcgagaga tcggaattgc
      121 tcagaactct tgtgcacggc gcgacaaagc cggaaccgcc gaaaattccg gcgatcaacc
      181 ggcaagctgc ttgatgcgct cggccaggcg gccgaaagcc tcgttcacgt attccagcac
      241 cttgggttct gcgccctgcg tgcgcagcga gtctttgaag gcttccaggt cggacagcca
      301 ttgccgatgc aagcggccca gctcgtcgag ctgaggccgg aagtcttgga gactgccgct
      361 cgcacgaaac gcctggtagc cacgctcaag cgtggccgcc gtggtcagca gttgcgcgcc
      421 catttgcttg tgctgttcgg agaactcggc catgccgtca tagggccgcg aagccttgcg
      481 gcgctcggct tcggatgcct cttccatgat gcgggcgtag cggtagaacc ctgggaactc
      541 gcgggacagc gccatgagct gctgatcgga tgtgccgacc caaatcctgt gcaggtcagg
      601 cccgtacccc atcatgcgat tgatgatggc atgggcctcg ctgacaccct gggcggcaag
      661 ctgctgcatg tgttggtcga tcttcgcggc cagtcggcgg aattcgttca tgctcatgtc
      721 cgtcacaaaa ggcgcgttga ttgcccttgc gccgtgagaa gtgccaccgc cttcttatcg
      781 aaggaaacga aggtttcccc gccaagccag ttgccttcgt aggcaatgac gccatcggca
      841 aagtctccgc ccgcgtcgag caccagcaag ccagcctcca cggcaggccg gttcacttcc
      901 acattcgcgg cggccagtag tgcccggatc gcgctggccg cgtcggcttg ctggaagccg
      961 tagacacgca gcagcaccca aacaaattcg cataggcacg gcagcgcgac cgcgatcaac
     1021 tcggcgtcgg tcaagactgc ggcggcaacg tccgcttgtg cgggatcgtc acgcacaacc
     1081 gcacgcacaa ggacgttggt atcgactgcg accttcattg cttacctgcc cagccttgcg
     1141 ccgccgcctc gttgatttct tcgatggtgg caaccttctg cgtcttgccc gcgagcaggc
     1201 cgacaaagct ggctatcgtc cctgcgggcc gtgccgcctt gagcacgccc cgaccgtctg
     1261 gcaacaagtc cagctcgatc ttgtcgcctg gcctgatgcc gaggtgttgc agtacgtcct
     1321 tccgaaacgt cacttgtccc cgtgcggtaa cggtcaatgt ggtcatggtg gtttgccctc
     1381 gcaatcaagg ttgatgcttc acatagtaat gcaaaaatgc cttaccgtca atgtccgtca
     1441 aacgatcatt agtcggccat gccggacact gtccagcaaa gttattgcac aacgggttgt
     1501 cggacattaa gataggcgga cggaatgacg gacaagagag gcggcaaatg gcactgatcg
     1561 gctatgcgcg ggtatcgacg gcggaacagg acaccgcctt gcagacggat gcgctacgca
     1621 aggcaggctg cgagcgcgtt ttcgaggaca cggcttccgg ggccaaggcc gaccgccccg
     1681 gcttggctga tgcgctggcc tacctgcgcg acggcgacgt gctggccgtc tggcggctgg
     1741 atcggctcgg gcgctctatg ccgcacctaa tcgaaacgat aggcgcgctg gaagcgcgag
     1801 gcgtcggctt ccgttctctg acggaagcca tcgacaccac cacgccaggc gggcggctca
     1861 tcttccacgt gttcggcgcg ctgggccagt tcgagcgcga cttgatccgc gagcgcacca
     1921 aggccgggtt gactgccgcc gccgctcgtg ggaggaaggg cgggcgaaag ccggttgtca
     1981 ccgccgacaa gttgcagcga gcgcgggagc acatcgccaa cgggcttaat gtccgagagg
     2041 ccgctacacg gctcaaggtg agcaagacgg ccctgtacac cgcgctgcaa tccaccagtg
     2101 cagccgactc ctgatattcc gtgcagtcgt cttctgaaaa tgacatcacg cgcaggtaga
     2161 tgcgtgcgac tttcatgcgg gcctcctggt cattttgggt atagggaaaa tgaccattgt
     2221 ttcacgccta gccaaaaagg gaaggttccc ggttcaaatg tcgttttcag aagacggctg
     2281 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatcc atcaggcaac
     2341 gacgggctgc tgccggccga ttctgttgaa aaagtagcgg cctctccata ccgttggcaa
     2401 aattgctctg tcagcgagcg tgggggcgaa cagcatgatg gggcagttac cgggaggaca
     2461 gcagcgcctg ttctactcgt tcaacctaga agatcacgtc ccggctcaac acctcctgcg
     2521 cagcatcgac cagtgcctgg atcttagtga tctgcgcgcc tacctggcgg acttctatag
     2581 tcccatcgga cgcccctcga ttgacccgga attgatggtg cgcatgctgg tcgtcggcta
     2641 ctgctatggc attcgttccg agcggcgatt gtgcgaagag gtgcacctga acctggccta
     2701 tcgctggttc tgccggctgg gtctggaaga cgaagtcccc aatcactcga ccttctcgaa
     2761 gaatcgccat gggcgttttc gtgacagcga tctgttccgc tggttgttca atgaggtgct
     2821 gcggcgttgc atggcagccg gcctagtcaa gggcgaaggt tttgccgtcg acgctagcat
     2881 catcaaggcg gatgccagcc ggcaacgtgg ggtagcggga gatgaggtcg attggaacga
     2941 tccaaagctc agcagccgcg cagtgcgcga gtacctcgaa gcccttgatg aagaggcgct
     3001 ggctgaggct cttcccaaga aaatttcgct cacagatcct cagtcccgtt ggacagcagc
     3061 gccaggtggg ccggcctttt ttgcctactc cacgaattac ctgatcgaca ctgagcacgg
     3121 tgtgatcatg gacgtggaag ctaccccggc gcaccgtacc gccgaagtcg attcgactag
     3181 gacgatggtt gagcgtgtca aagctcagtt cgatctcaca ccggaacgcc ttatcggcga
     3241 taccgcttat ggcaccgccc cgatgctggc ctggatggtc gaagaaaagg acatcgagcc
     3301 gcatgtgccg gtgtgggaca agactgagcg caaggacgac agcctctcca gtaacgactt
     3361 ccactggaac caggaggcca atgaataccg ctgcccagcc ggcaaaccac tacgcagtga
     3421 gtggcgcgcc ttcacccaga aaagatcgcg ggtgaccaag gcccacacca tcatttatcg
     3481 ctccagccaa accgactgca ctacctgtcc gctgaaagcg aaatgctgcc ccaacacgcc
     3541 gaatcggaag atcgtccgca gtatccatga ggctgcccgc gatgtggcca ggcgcatcgc
     3601 caagacaccg gaatacctcg tctctcgctg cgaacgaaag aaggtggaga tgctgttcgc
     3661 ccacctcaaa cggatcatga aactcgatcg tttacgactg cgcggcctga caggtgccac
     3721 tgacgaattc actttggcgg cggcggtgca gaacctgcga cgcatggcca agcttttgcc
     3781 tcaagggcca ccgctcacgg gataggtacg cctgctgaga gcagaaacct tcaaattaac
     3841 cctcaggcct gagcaaggac gctcagcgaa acgccgaaag gcaacctgaa gtggcttgca
     3901 gtcacttcga cagcaggcac acctgatcgg caggccgccg ctaaacctac tttttcaaca
     3961 gaatcggcca tcagcggacg cagggaggac tttccgcaac cggccgttcg atgcggcacc
     4021 gatggccttc gcgcaggggt agtgaatccg ccaggattga cttgcgctgc cctacctctc
     4081 actagtgagg ggcggcagcg catcaagcgg tgagcgcact ccggcaccgc caactttcag
     4141 cacatgcgtg taaatcatcg tcgtagagac gtcggaatgg ccgagcagat cctgcacggt
     4201 tcgaatgtcg taaccgctgc ggagcaaggc cgtcgcgaac gagtggcgga gggtgtgcgg
     4261 tgtggcgggc ttcgtgatgc ctgcttgttc tacggcacgt ttgaaggcgc gctgaaaggt
     4321 ctggtcatac atgtgatggc gacgcacgac accgctccgt ggatcggtcg aatgcgtgtg
     4381 ctgcgcaaaa acccagaacc acggccagga atgcccggcg cgcggatact tccgctcaag
     4441 ggcgtcggga agcgcaacgc cgctgcggcc ctcggcctgg tccttcagcc accatgcccg
     4501 tgcacgcgac agctgctcgc gcaggctggg tgccaagctc tcgggtaaca tcaaggcccg
     4561 atccttggag cccttgccct cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt
     4621 gacccgcagt tgcaaaccct cactgatccg catgcccgtt ccatacagaa gctgggcgaa
     4681 caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc acttcatccg gggtcagcac
     4741 caccggcaag cgccgcgacg gccgaggtct tccgatctcc tgaagccagg gcagatccgt
     4801 gcacagcacc ttgccgtaga agaacagcaa ggccgccaat gcctgacgat gcgtggagac
     4861 cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa
     4921 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc
     4981 ctgttcggtt cgtaagctat aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     5041 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     5101 tttttttgta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     5161 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     5221 gttaggcacc aatggatagt tcgccgctcg tcaggcctgt tgaaactacc gattcggcca
     5281 gttggctaag catgcgctgt gagctgtggc cagatggcac atgtcaagag caccagtcag
     5341 agatcgcaga atttctgtcc ggaaaagtcg cccggcctgc tgctgtcctc attgctgtag
     5401 cacccgacgg agaagcacta gggtttgccg agctttcgat ccgcccgtat gcggaggagt
     5461 gctactccgg caacgttgcg ttcttggagg gttggtacgt tgtgccaagt gcgcggcgtc
     5521 agggcgtagg tgtagctctg gtaaaagccg ccgagcattg ggctcgtggt cgcggatgca
     5581 ccgaattcgc ctccgacact caacttacca acagcgcaag cacctcggcg cacctggcgg
     5641 ctggattcac ggaggttgct caagtacgct gcttccggaa accgttgtga ggggcgccgc
     5701 gttggtgcct aacaattcgt tcaagccgaa cttgcttcgt tacaccaaag ccatggcaga
     5761 aagagcttgc catggctttg gctccactac gcaagtcggc ttaactcagg cgttagaaaa
     5821 ggaaaagtat gagcaagtta tctgtattct ttatattttt gttttgcagc attgctaccg
     5881 cagcagagtc tttgccagat ttaaaaattg aaaagcttga tgaaggcgtt tatgttcata
     5941 cttcgtttga agaagttaac gggtggggcg ttgttcctaa acatggtttg gtggttcttg
     6001 taaatgctga ggcttatcta attgacactc catttacggc taaagatact gaaaagttag
     6061 tcacttggtt tgtggagcgt ggctataaaa taaaaggcag tatttcctct cattttcata
     6121 gcgacagcac gggcggaata gagtggctta attctcgatc tatccccacg tatgcatctg
     6181 aattaacaaa tgaactgctt aaaaaagacg gcaaggttca agccacaaat tcatttagcg
     6241 gagttaacta ttggctagtt aaaaataaaa ttgaagtttt ttatccaggc ccgggacaca
     6301 ctccagataa cgtagtggtt tggctgcctg aaaggaaaat attattcggt ggttgtttta
     6361 ttaaaccgta cggtttaggc aatttgggtg acgcaaatat agaagcttgg ccaaagtccg
     6421 ccaaattatt aaagtccaaa tatggtaagg caaaactggt tgttccaagt cacagtgaag
     6481 ttggagacgc atcactcttg aaacttacat tagagcaggc ggttaaaggg ttaaacgaaa
     6541 gtaaaaaacc atcaaaacca agcaactaaa tttctaacaa gtcgttgcag cacgccacta
     6601 cgtggctgga cagtttgtaa gttgcgcttt tgtggtttgc ttcgcaaagt attccacaac
     6661 gcgcaactta caaactgccg ctgaacttag cgttgcgcgc cggcactcgc agtttttgag
     6721 ccttgcacac ctgagtgact tcgcggtgaa aggccgctag gctgcgcttc tgcttggtca
     6781 ggaaccgctt ttgcagtagc tcgtggatga cgcgctcgac cggttccggc aagcgcccct
     6841 tacctttacc tccaccggac tggccgggca ccagatccgt cacgaggccg ctgccttgcc
     6901 gggcacgccg gatcagaacg tatacctggc gccgagacaa gcccagcgcc tgagccgcca
     6961 tatcggccgc ttcgtgcccg accgtctccg actgcgccaa cggactgatg atctccgcac
     7021 gacggcgcgc acgctcccaa gcctcatcag gcagagtggc cacgccttgt tctggaatcc
     7081 gtggggtgtc cgtcgccatg ctcacctcgc tttggtgcac acgagtattg agcatagtcg
     7141 agattggtgc agatcacttc tgatattgaa ctgtcaggag ctggctgcac aacagccatt
     7201 acgcccaatc aactggtgca gtcgtcttct gaaaatgaca
//
