LOCUS       Tn6363                  6066 bp    DNA     linear       29-SEP-2020
DEFINITION  Cronobacter sakazakii.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Cronobacter sakazakii
  ORGANISM  Cronobacter sakazakii
            Unclassified.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn6363
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Cronobacter sakazakii"
                     /mol_type="genomic DNA"
     repeat_region   1..18
                     /gene="IRL_IS903B"
     CDS             33..1001
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS903B transposase"
                     /translation="MKDQITHLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSNTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
     repeat_region   1040..1057
                     /gene="IRR_IS903B"
     CDS             1162..1977
                     /gene="aphA1"
                     /codon_start=1
                     /product="Aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNLDVDLYGYRWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2114..3007)
                     /gene="orf894"
                     /codon_start=1
                     /product="LysR family transcriptional regulator"
                     /translation="-
                     DELKVFLTVARLKSFTKAAAEIGVTPSAISHTVKSLEERLGIRLLARTTRSISTTEAG
                     ERLINEVGPLMEQVDDAISRLSELREKPTGVIRLTAADDVIQYLIRPVLPDFLSRYPD
                     IQIEIGIDYGFTDIVDQRFDAGIRPGDVLNNDMIAAKISQNWRQLVVAAPDYFKRYAK
                     PRKPQDLLNHNCINVRYSDTSGLYAWEFEKDAQKFSLKVKGQYIANSTIHQLDAALDG
                     LGIAYIPEYVADGYIKSGKLIAVLTEWCPYFDGYHIYYPHRRQDSPAFMAFLQLLRDR
                     YNNGIR"
     CDS             3201..4358
                     /gene="yqhD"
                     /codon_start=1
                     /product="Alcohol dehydrogenase YqhD"
                     /translation="MQDFIYHNPVKILFGRDQISALAQEVPQGKKVMIVYGGGSVIKH
                     GILKRVKESLTNTLVYEFGGVEANPHYETLMKAVEIVRTEKIDFLLAVGGGSVIDGTK
                     FIAAAALYENDPWEIVKSYGGVVKQALPFGCVLTLAATGSEMNNTAVINRVTTQDKLF
                     FTSPYVMPKFSVLEPEMTYTLPEQQTANGVVDAFVHVLEQYITYPVNAKVQDRLAEGL
                     LSTLKEEGPVALKNPQNYDARANIMWAATMALNGMLSAGVPTDWASHMIGQEITALYG
                     IDHARTLAIVMPALWRLCKKEKAEKLAQYGARVWNIPESDSEKMADEAINATVEFFEL
                     MGVKTRLSDYGLGAEDISAVIGKLKEHGHIALGEHGKITAEVAETILNMAL"
     CDS             4436..5020
                     /gene="orf585"
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MQKMNDTFPYMPLMAFAMTGFICIMTETIPAGLLPEISKGMNIS
                     LASAGQWVTVYALGSLFAAIPLTIVTRSWNRKYVLLMTVAGFFIFNTITALSSNVYLT
                     LLARLGAGAAAGLAWSLLAGFSRRIVEPLHQGRAMAIAMAGTPLALSLGVPLGTWLGH
                     YLGWRLTFGIMSVLSLLLMIWIRISVPDSAGRLC"
     repeat_region   5010..5027
                     /gene="IRL_IS903B"
     CDS             5042..6010
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS903B transposase"
                     /translation="MKDQITHLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSNTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
     repeat_region   6049..6066
                     /gene="IRR_IS903B"
ORIGIN      
        1 ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg
       61 acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg cccacctaca
      121 ataaagccct catcaaccgt ggctccataa ctttctggct ggatgatgaa gctattcagg
      181 cctggtatga gtcagcaaca ccttcttcac gaggcagacc tcagcgctat tctgaccttg
      241 ccatcacgac tgtgctggtc attaaacgcg tattcaggct gaccctgcgc gctgcgcagg
      301 gctttattga ttccattttt tctctgatga acgttccgct acgctgcccg gattacagct
      361 gtgtcagcag gcgggcaaag tcggttaatg tcagtttcaa aacgcccacc cggggtgaaa
      421 tcgcacacct ggtaattgat tccaccgggc tgaaggtctt cggtgaaggc gagtggaaag
      481 tcaaaaagca tggccaggaa cgccgtcgta tctggcgaaa gttgcatctg gcagttgaca
      541 gcaacacaca tgaaatcatc tgtgcagacc tgtcgctgaa caacgttacg gactcagaag
      601 ccttcccggg tcttatccgg caaacccacc ggaaaatcag gtcagccgcc gccgatggcg
      661 cttacgatac ccggctatgt cacgatgaac tgcggcgtaa gaaaatcagc gcgcttatcc
      721 ctccccgaaa aggtgcgggt tactggcccg gtgaatatgc agaccgtaac cgtgcagtgg
      781 ctaatcagcg aatgaccggg agtaatgcgc ggtggaaatg gacaacagat tacaaccgtc
      841 gctcgatagc ggaaacggcg atgtaccggg taaaacagct gttcgggggt tcactgacgc
      901 tgcgtgacta cgatggtcag gttgcggagg ctatggccct ggtacgagcg ctgaacaaaa
      961 tgacgaaagc aggtatgcct gaaagcgtgc gtattgcctg aaaacacaac ccgctacggg
     1021 ggagacttac ccgaaatctg atttattcaa caaagccacg ttgtgtctca aaatctctga
     1081 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1141 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg ctcgaggccg
     1201 cgattaaatt ccaacctgga tgttgattta tatgggtata gatgggctcg cgataatgtc
     1261 gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt
     1321 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1381 tggctgacgg catttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1441 gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat
     1501 cctgattcag gtgaaaatat tgttgatgcg ctggcagcgt tcctgcgccg gttgcattcg
     1561 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     1621 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     1681 cctgttgaac aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc
     1741 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     1801 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     1861 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     1921 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     1981 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgct
     2041 tccctaccct catggccgcc tcccaggaat cgacccaggt gcttatcgtg gacataatcc
     2101 tttactcata aatttatctt attccattat tgtacctgtc gcgcaaaagt tgcagaaaag
     2161 ccataaacgc aggagaatcc tgtcggcggt gaggataata aatatggtag ccgtcaaaat
     2221 atggacacca ctcggtcaga acagcaatca gcttgccgct tttgatatat ccatctgcaa
     2281 catactcggg aatataggca atccccagcc cgtctaatgc tgcatcgagc tgatgaatcg
     2341 tgctgttcgc aatatattgc cctttgactt tcagactgaa tttctgagca tctttctcga
     2401 attcccaggc gtacaatcca ctcgtatcag aatagcggac gttaatgcag ttgtggttca
     2461 aaagatcctg cggcttcctg ggcttagcat aacgcttaaa ataatctggt gcagcaacaa
     2521 caagctggcg ccagttctgg cttatttttg cggcaatcat atcattattc agaacatcgc
     2581 ccggacgaat cccggcatca aaacgctgat caacaatatc tgtaaatcca tagtcaatgc
     2641 cgatttcaat ctgaatatct gggtagcgac tcagaaagtc cggtaataca ggacggatca
     2701 aatactggat aacatcatct gccgccgtga ggcgtatgac gccagtgggt ttttccctaa
     2761 gctcactcaa ccgactaata gcatcatcaa cctgttccat taacgggcca acttcgttaa
     2821 tgagcctttc acctgcttcc gtagtggaga tactgcgggt ggttctggcc agcaggcgga
     2881 tcccaagcct ttcctcaaga gatttcaccg tgtggctgat ggctgagggt gtcacaccta
     2941 tttcagctgc ggctttggta aagcttttca gcctggccac tgttaaaaaa acctttaatt
     3001 catccacacg attcctcctc ctcggtccct actggagacg tcagcgtacc agtctgtcat
     3061 taattatcaa ataaatttca caagtaagtg aaatttatgt tgattaataa ataaaattca
     3121 aaatggtact ttatattcca cgacgtgaat atgtcctcgt tgtagtgacg gcttttaatt
     3181 ctgaaaatga ggtaatcatt atgcaagatt ttatctatca taatccggta aaaattcttt
     3241 ttggtcgcga tcaaatttct gcgttagcac aagaagtccc gcagggtaaa aaggtcatga
     3301 ttgtctatgg cgggggcagc gttataaaac acggtatatt gaaacgcgta aaagagtccc
     3361 tgacaaacac cctggtttat gagtttggcg gtgtagaagc aaaccctcat tatgaaaccc
     3421 tgatgaaagc cgttgagatt gtcagaacag aaaaaattga ttttcttctg gccgtgggcg
     3481 gcggttcagt gatcgacggg actaaattta ttgccgccgc agccctctac gaaaatgatc
     3541 catgggagat cgtcaagagt tatggcggcg tggtgaaaca agctctccct tttggatgcg
     3601 tgctgaccct ggcggccacg ggttcagaga tgaataacac ggcggtgatt aatcgggtga
     3661 ccacccagga taaactcttt ttcacctccc cttacgttat gccgaaattt tcagtgctcg
     3721 aaccggaaat gacctacacg ctgcctgaac aacaaaccgc aaacggggtt gttgatgcct
     3781 ttgtccacgt tcttgagcaa tacattactt acccggttaa cgctaaggtg caggatcgcc
     3841 tggcggaagg actgctgagc acgctgaaag aagaaggccc agtagccctt aaaaatccac
     3901 agaactacga cgccagagct aacatcatgt gggctgcgac gatggccctg aatggaatgt
     3961 tgagcgcagg tgtcccaact gactgggctt ctcacatgat tggccaggaa attactgcat
     4021 tatacggaat cgaccacgcc cgtaccctcg ctatcgttat gcctgcacta tggcgcttgt
     4081 gcaaaaagga aaaagcagaa aagctggctc agtacggtgc gcgtgtctgg aatattcctg
     4141 agtctgatag cgaaaaaatg gccgatgagg ctatcaatgc caccgtggaa ttttttgaac
     4201 tgatgggcgt taaaacccga ctttctgatt atggtctggg cgcggaagat atttcagctg
     4261 ttatcggcaa gttaaaagaa catgggcaca tcgcattagg tgaacatgga aaaattactg
     4321 ctgaagtggc ggaaacaata ctgaacatgg cactttaatt gactaacgat ctactctatt
     4381 aggcgctaag cctgcgcacc tggcaggctt aattaaacat taacggtatt gcaaaatgca
     4441 gaaaatgaat gacacatttc cctatatgcc attaatggct tttgcgatga ccggatttat
     4501 ttgcatcatg acggaaacta ttccagcagg attattgcca gaaatcagta agggaatgaa
     4561 tatttcgctg gctagcgcag gccagtgggt caccgtctac gccctcgggt cattatttgc
     4621 cgctattccg ttaactatcg ttacccggtc gtggaatcgg aaatatgtct tattaatgac
     4681 cgtagcggga ttcttcattt ttaataccat taccgcactt tcatcaaatg tttacctgac
     4741 cctgttagcg cgtttggggg ccggggcggc tgcgggactg gcgtggagcc tgctggcggg
     4801 gttttcacgc cgaattgttg agccgcttca tcagggtcgg gctatggcga tagccatggc
     4861 tgggacaccg ctggcgctct cactaggcgt tccgctggga acctggcttg ggcattatct
     4921 tggctggcgc ctgacgttcg gtattatgtc agtactttcg ctgttactaa tgatatggat
     4981 ccgtatcagc gttccggact ctgcggggcg gctttgttga ataaatcgaa cttttgctga
     5041 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa
     5101 gttcaaaatc accaactggc ccacctacaa taaagccctc atcaaccgtg gctccataac
     5161 tttctggctg gatgatgaag ctattcaggc ctggtatgag tcagcaacac cttcttcacg
     5221 aggcagacct cagcgctatt ctgaccttgc catcacgact gtgctggtca ttaaacgcgt
     5281 attcaggctg accctgcgcg ctgcgcaggg ctttattgat tccatttttt ctctgatgaa
     5341 cgttccgcta cgctgcccgg attacagctg tgtcagcagg cgggcaaagt cggttaatgt
     5401 cagtttcaaa acgcccaccc ggggtgaaat cgcacacctg gtgattgatt ccaccgggct
     5461 gaaggtcttc ggtgaaggcg agtggaaagt caaaaagcat ggccaggaac gccgtcgtat
     5521 atggcgaaag ttgcatctgg cagttgacag caacacacat gaaatcatct gtgcagacct
     5581 gtcgctgaac aacgttacgg actcagaagc cttcccgggt cttatccggc agactcacag
     5641 aaaaatcagg gcagccgcgg cagacggcgc ttacgatacc cggctctgtc acgatgaact
     5701 gcggcgtaag aaaatcagcg cgcttatccc tccccgaaaa ggtgcgggtt actggcccgg
     5761 tgaatatgca gaccgtaacc gtgcagtggc taatcagcga atgaccggga gtaatgcgcg
     5821 gtggaaatgg acaacagatt acaaccgtcg ctcgatagcg gaaacggcga tgtaccgggt
     5881 aaaacagctg ttcgggggtt cactgacgct gcgtgactac gatggtcagg ttgcggaggc
     5941 tatggccctg gtacgagcgc tgaacaaaat gacgaaagca ggtatgcctg aaagcgtgcg
     6001 tattgcctga aaacacaacc cgctacgggg gagacttacc cgaaatctga tttattcaac
     6061 aaagcc
//
