LOCUS       Tn6346                  8230 bp    DNA     linear       29-SEP-2020
DEFINITION  Achromobacter sp.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Achromobacter sp.
  ORGANISM  Achromobacter sp.
            Unclassified.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn6346
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Achromobacter sp."
                     /mol_type="genomic DNA"
     repeat_region   1..38
                     /gene="IRL_Tn6346"
     CDS             complement(34..3000)
                     /gene="tnpA"
                     /codon_start=1
                     /product="Tn6346 transposase"
                     /translation="MPRRSILSAAERESLLALPDTKDELIRHYTFSETDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDEPPFPPLLKLVADQLKVSVESWDEYGQREQTRRE
                     HLVELQTVFGFQPFTMGHYRQAVQLLTEMALQTDKGIVLASTLIEHLRQQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLSDAHRRRLDDLLKRRDNGKTTWLAWLRQSPVKP
                     NSRHMLEHIERLKAWQALDLPSGIERSVHQNRLLKIAREGGQMTPADLAKFEAQRRYA
                     TLVALAIEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLFGRIG
                     QALIEAKQAGRDPFAAIEAVMSWDAFAESVTEAQKLAQPEDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKDVLDAIEVLRGMNSDNARKVPADAPTEFIKPRWQKLVMTDT
                     GIDRRYYELCALSEMKNALRSGDIWVQGSRQFKDFEDYLVPPAKFASLKQASELPLAV
                     ATDCNRYLNDRLTLLETQLATVNRMATANELPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMILPHVKITELLLEVDEWTGFTRHFAHLKSGDPAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHIRDETYGAALADLVNAQFRHPFAEHWGDGTTSSSDGQ
                     NFRTGSKAESTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGVRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAVVLWNTVYLERAAHALRGNGHAVDDALLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     CDS             complement(3003..3563)
                     /gene="tnpR"
                     /codon_start=1
                     /product="Tn6346 resolvase"
                     /translation="MHGQRIGYVRVSSFDQXPERQLEQIQVDKVFTDKASGKDTRRPE
                     LERLLAFVREGDTVVVHSMDRLARNLDDLRRLVQGLTQRGVRIEFLKEHLTFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     misc_recomb     3585..3704
                     /gene="res"
     CDS             complement(3694..4683)
                     /gene="urf2"
                     /codon_start=1
                     /product="Diguanylate phosphodiesterase"
                     /translation="MSAFRPDGWTTPELAQAVERGQLELHYQPVVDLRSGGIVGAEAL
                     LRWRHPTLGLLPPGQFLPVVESSGLMPEIGAWVLGEACRQMRDWRMLAWRPFRLAVNV
                     SASQVGPDFDGWVKGVLADAELPAEYLEIELTESVAFGDPAIFPALDALRQIGVRFAA
                     DDFGTGYSCLQHLKCCPISTLKIDQSFVAGLANDRRDQTIVHTVIQLAHGLGMDVVAE
                     GVETSASLDLLRQADCDTGQGFLFAKPMPAAAFAVFVSQWRGATMNASDSTTTSCCVC
                     CKEIPLDAAFTPEGAEYVEHFCGLECYQRFEARAKTGNETDADPNACDSLPSD"
     CDS             complement(4680..4916)
                     /gene="merE"
                     /codon_start=1
                     /product="Mercuric resistance protein MerE"
                     /translation="MNNPERLPSETHKPITGYLWGGLAVLTCPCHLPILAVVLAGTTA
                     GAFLGEHWVIAALGLTGLFLLSLSRALRAFRERE"
     CDS             complement(4913..5278)
                     /gene="merD"
                     /codon_start=1
                     /product="Mercuric resistance protein MerD"
                     /translation="MNAYTVSRLALDAGVSVHIVRDYLLRGLLRPVACTPGGYGLFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGDEAAAQLALLRQFVERRREALADL
                     EVQLATLPTEPAQHAESLP"
     CDS             complement(5296..6981)
                     /gene="merA"
                     /codon_start=1
                     /product="Mercuric reductase MerA"
                     /translation="MTHLKITGMTCDSCAAHVKEALEKVPGVQSALVSYPKGTAQLAI
                     VPGTSPDALTAAVAGLGYKATLADAPLADNRVGLLDKVRGWMAAAEKHSGNEPPVQVA
                     VIGSGGAAMAAALKAVEQGAQVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRES
                     PFDGGIAATVPTIDRSKLLAQQQARVDELRHAKYEGILGGNPAITVVHGEARFKDDQS
                     LTVRLNEGGERVVMFDRCLVATGASPAVPPIPGLKESPYWTSTEALASDTIPERLAVI
                     GSSVVALELAQAFARLGSKVTVLARNTLFFREDPAIGEAVTAAFRAEGIEVLEHTQAS
                     QVAHMDGEFVLTTTHGELRADKLLVATGRTPNTRSLALDAAGVTVNAQGAIVIDQGMR
                     TSNPNIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALDLTAMPAVVFTDPQVATV
                     GYSEAEAHHDGIETDSRTLTLDNVPRALANFDTRGFIKLVIEEGSHRLIGVQAVAPEA
                     GELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     CDS             complement(7053..7328)
                     /gene="merP"
                     /codon_start=1
                     /product="Mercuric transport protein periplasmic component
                     MerP"
                     /translation="MKXLFASLALAAVVAXVWAATQTVTLSVPGMTCSACPITVKKAI
                     SKVEGVSKVDVTFETRQAVVTFDDAKTSVQKLTKATADAGYPSSVKQ"
     CDS             complement(7341..7691)
                     /gene="merT"
                     /codon_start=1
                     /product="Mercuric transport protein MerT"
                     /translation="MSEPKTGRGALFTGGLAAILASACCLGPLVLIALGFSGAWIGNL
                     AVLDPYRPIFIGVALVALFFAWRRIYRQAAACKPGEVCAIPQVRATYKLIFWIVAALV
                     LVALGFPYVMPFXY"
     CDS             7763..8197
                     /gene="merR"
                     /codon_start=1
                     /product="Mercuric regulatory protein MerR"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLLEPDKPYGSIR
                     RYGEADVTRVRFVKSAQRLGFSLDEIAELLRLEDGTHCEEASSLAEHKLKDVREKMAD
                     LARMEAVLSELVCACHARRGNVSCPLIASLQGGASLAGSAMP"
     repeat_region   8193..8230
                     /gene="IRR_Tn6346"
ORIGIN      
        1 ggggtcgcct cagaaaacgg aaaataaagc acgctaagcc ggttgcagcg gtcgtagcgg
       61 cctgaacttg cccgcgccga tcttggcgct gctgcgccag aggtaatcgc cggtgaggtt
      121 gatgtgctcc caaccgagcg gcgacaggta ctgcaacagc gcgtcatcaa cggcatggcc
      181 gttgccacgc agcgcgtgcg cagcccgttc cagatagacc gtgttccaca acacgacggc
      241 agccgttacc agattgaggc cgctagcccg gtagcgctgc tgctcgaaac tgcggtcgcg
      301 gatttcaccc aggcggttga aaaacactgc cctggccagc gcattgcgcg cctcgccctt
      361 gttcaggccg gcatgcacgc ggcggcgcag ttccacgctt tgcagccagt ccaggatgaa
      421 cagcgtgcgc tcgatgcggc ccagctcgcg gagcgccacg gccaggccgt tctggcgtgg
      481 gtagctgccg agctttcgga gcatcaggga ggccgtcacc gtgccctgct tgatcgaggt
      541 ggccagccgc aggatttcgt cccaatgggc gcggacgtgc ttgatgttga gcgtgccgcc
      601 gatcatgggt ttcagcgcgt cataggcggc gtcgcccttc gggatgtaga gcttggtgtc
      661 gcccaggtcg cggatgcgcg gcgcgaagcg gaagcccagg aggtgcatca gggcgaagac
      721 gtgatcggtg aagcccgccg tgtcggtgta atgctcctcg atccgcaagt cggactcgtg
      781 gtacagcagg ccgtcgagca cgtaggtcga atcgcgcacg ccgacgttca cgaccttggt
      841 gtgaaatggc gcgtactggt cagaaatgtg ggtgtagaac gtccgccctg ggctgctccc
      901 gtatttcggg ttgatgtggc cggtgctctc ggccttgctg ccggtgcgga agttctggcc
      961 gtccgacgat gaggtggtgc cgtcgcccca gtgctcggcg aagggatggc ggaactgtgc
     1021 gttgaccaga tcggccagcg ccgccccgta ggtttcgtcg cggatgtgcc aggcttgcag
     1081 ccaagccagc ttggcgtagg tcgtgccggg gcaagactcc gccatcttgg tcaggcccag
     1141 gttgatcgcg tcggcgagga tcgtggtcag caacaggttc ttgtctttgg ccgggtcgcc
     1201 cgatttcaga tgcgcgaaat gccgagtgaa gcccgtccat tcgtccacct ccagcagcag
     1261 ttcggtgatc ttgacgtgcg gcaggatcat tgccgtctgg tcgatcagcg cttgggcggt
     1321 gtcgggtacc gccgcgtcga gcggcgtgat cttcaagcct gactcggtga tgatggcgtc
     1381 cggcagctcg ttggccgtcg ccatacggtt gacggtggca agctgtgttt ccagcagcgt
     1441 cagccggtcg ttcaggtacc ggttgcagtc ggtggccacg gccagcggca attcgctggc
     1501 ctgcttgagg ctggcgaatt tcgcgggcgg caccaggtag tcctcgaagt ccttgaactg
     1561 gcgcgacccc tgcacccaga tgtcgccgga acgcaacgcg ttcttcatct ccgacagcgc
     1621 gcacagttcg tagtagcgcc ggtcgatgcc ggtgtcggtc atgaccagct tctgccagcg
     1681 cggcttgatg aactcggtcg gcgcgtcggc gggcaccttg cgggcgttgt cgctgttcat
     1741 gccgcgcagc acctcgatgg cgtcgagcac gtccttcgcg gcgggagcgg cccgcagctt
     1801 gagcacggca aggaattccg gcgcgtagcg gcgcagcgtg gcgtagcttt cgccgatgcg
     1861 gtgcaggaaa tcgaagtcct cgggctgcgc aagcttctgc gcttcggtga cgctctcggc
     1921 gaaggcatcc caggacatga cggcctcgat ggcggcgaac ggatcgcggc ccgcctgctt
     1981 ggcctcgatc agcgcctggc cgatgcggcc gaacagccgc accttggcgt tgatcgcctt
     2041 gccggacgcc tggaattgct gctgatgctt gttcttggcg gcgttgaaca gcttgcccag
     2101 gatgcggtcg tgcaggtcga tgatttcgtc ggtgacggtg gccatgccct cgatggcaag
     2161 cgccaccagg gtggcatagc gtcgctgcgc ctcgaacttg gccaggtcgg cgggcgtcat
     2221 ctggccaccc tcacgggcga tcttgagcag gcggttctgg tgcaccgacc gctcgatgcc
     2281 agaaggcagg tcgagcgcct gccacgcttt gaggcgttcg atgtgttcca gcatgtgccg
     2341 cgaattcggt ttgacgggcg attggcgcag ccaggccagc caggtcgttt tgccgttgtc
     2401 ccgacgcttg agcagatcgt cgaggcggcg gcgatgcgcg tccgacagcg gttcggccaa
     2461 ggcatcgtag atgcgccggt tggcgcgggt gattgcttcg gcgctcgccc gctcgacggc
     2521 gttgagggca ggcagaatga ccgactgctg ccgcaggtgc tcgatcaagg tgctggccag
     2581 cacgatgccc ttgtcggtct gcaaggccat ctcggtcagc aactggacgg cctgccggta
     2641 gtggcccatg gtaaagggct ggaagccgaa caccgtttgc agttcgacca ggtgctcgcg
     2701 ccgggtctgc tcccgctgcc cgtattcgtc ccagctttcg acgctgacct tgagctggtc
     2761 ggcgaccagt ttcaacaagg gcggaaacgg cggctcatcg acgcccagga tgacaccagg
     2821 aaagcgcagg taacagagct gcacggcgaa gcccagccgg ttggccgggc cgcgccgctg
     2881 ccggatgatg gagaggtcgg tttcgctgaa cgtgtagtga cggatcaact catccttggt
     2941 gtccggcaac gccagcaggc tttcgcgctc ggcggcggac aggattgaac gacgtggcat
     3001 atttactgat ccgttctcaa gtattgatac agggtttcgc gactgattcc gaattcacga
     3061 gccagcttgg tcttttgctc gccagcctcg acacgttggc gcagttcggc aatacgctca
     3121 gacgacaggg atttcttcct gccacggtag gccccgcgct gcttggcgag cgcgatgccc
     3181 tcgcgctgcc gctcgcggat caaggcgcgt tcgaactcgg cgaacgcgcc cattaccgac
     3241 agcatcaggt tcgccatcgg cgagtcctcg ccggtgaagg tcaaatgctc cttaaggaac
     3301 tcgatgcgta cgccgcgctg ggtgaggccc tgcaccaggc ggcgcaggtc gtcgaggttg
     3361 cgcgccagac gatccatgct gtgcaccacg accgtgtcgc cttcgcgcac gaaggcgagc
     3421 agccgttcca gttcgggccg ccgtgtgtcc ttgcccgacg ccttgtcggt gaacacttta
     3481 tccacctgga tctgttcgag ctgccgttct gggwtctggt cgaagctgct gacgcggacg
     3541 taaccgatgc gctgaccgtg caaggtatcc tcctgaggga aatgtgtcag gaagaaatct
     3601 atgacccttg acggcgtatg tcaatcaatt cggaaggcaa ctctattctg acgatttagc
     3661 gccggatggt ctgacgccaa gttagggtat gcctcaatct gacggtagcg agtcgcaggc
     3721 gttcggatcg gcatcggttt cgttccctgt cttggcacgc gcttcgaagc gttgataaca
     3781 ctccaacccg cagaagtgct cgacgtattc cgcgccttcc ggggtgaagg cggcatcgag
     3841 cgggatttcc ttgcagcaca cgcagcaact ggtggtggtc gagtcacttg cattcatggt
     3901 ggcacccctc cattgactga cgaagacggc gaatgccgcc gccggcattg gcttcgcgaa
     3961 caggaagcct tgtcctgtgt cgcagtccgc ttgtcgcaat agatcaagac tcgccgatgt
     4021 ttccacgcct tcagccacca catccatgcc cagcccgtgc gcaagctgaa tcacggtgtg
     4081 cacgatggtt tggtcgcggc ggtcgttggc gagcccggcg acaaacgatt ggtcgatctt
     4141 gagcgtgctg attgggcagc acttcagatg ttgcagacag gaataccccg tcccgaagtc
     4201 atcggcggcg aagcgcacac cgatctgccg caaggcgtcc agggcgggga agatcgccgg
     4261 atcaccaaac gcgaccgatt cggtcagctc gatttcgaga tactcggcgg gcaactcggc
     4321 atcagccagc acgcccttta cccacccgtc gaagtccggt cccacttggc tcgccgaaac
     4381 attgacggcc agccggaacg gtcgccatgc cagcattcgc cagtcacgca tctggcggca
     4441 ggcttcgccc agcacccaag cgccgatttc aggcatcagg ccggacgatt cgaccacggg
     4501 caggaactgg cccggtggca atagtccaag cgtcggatga cgccagcgca acagggcttc
     4561 cgcgccgaca atcccaccac tgcgcagatc gacgacgggc tggtagtgca gttcaagctg
     4621 cccgcgctcg accgcttggg ccagttccgg cgtcgtccat ccatccggcc ggaaagcgct
     4681 cattctcttt ccctgaatgc ccgcaacgcc cgcgacaggg acagaaggaa caggccggtc
     4741 aaaccgagcg ccgcgatgac ccaatgctcg ccgaggaaag caccggcggt tgtgccggcc
     4801 agcacgacag cgaggatggg caggtggcag gggcaagtca gcacagccag tccgccccac
     4861 aggtagccgg tgatcggttt gtgcgtctcg gacggcaagc gctcggggtt gttcatggca
     4921 gactctccgc gtgctgtgcc ggctcggtcg gcagggtggc caactgcact tccagatcgg
     4981 ccaacgcttc gcgccgacgc tcgacgaact gacgcagcag ggcaagctgc gcggccgctt
     5041 cgtcgccgtc cgccgcatcc agcgcccggc acagccgcgc cagcgcgtcg aggccgatgc
     5101 ccgcctcgaa ggccgcccgc acgaagcaca gccgttgcaa ggcggcgtca tcgaacaagc
     5161 cgtagccgcc tggtgtgcac gccaccgggc gcagcaatcc gcgcagcagg tagtcgcgca
     5221 cgatatgcac gctcaccccg gcatcaagag ccagccggga caccgtgtag gcgttcattg
     5281 aacacctcct ttttctcacc cggcgcagca ggaaagctgc ttcacatcct tgttgaaggt
     5341 ctgcgccgcg agcttcaacc cctcgaccat cgtcaggtag gggaacaact ggtcggccag
     5401 ttcctgcacc gtcatgcggt tgcgaatggc cagagccgcc gtctggatca gttcacccgc
     5461 ttccggcgcg accgcctgta cgccgatcag ccgatggctg ccttcctcga taaccaactt
     5521 gatgaagccg cgtgtgtcga agttggcgag cgcacgcggc acgttgtcca aggtcaaggt
     5581 gcggctgtcg gtctcgatcc cgtcgtggtg ggcttccgcc tcgctgtagc ccacggtcgc
     5641 cacttgcgga tcggtgaaca ccacggccgg cattgcggtc aggtcgagcg ccgcatcgcc
     5701 gccggtcatg ttgatcgcgg cacgggtgcc ggccgctgcc gccacataga cgaactgcgg
     5761 ctggtcggtg cagtcgccgg ccgcgtagat gttcgggttg ctcgtgcgca tgccttggtc
     5821 gatgacgatg gcaccttgcg cattgacagt gacccccgct gcgtccagcg cgaggctgcg
     5881 cgtgttcggt gtccgaccgg tggcaaccag cagtttgtcg gcgcgcaatt caccgtgcgt
     5941 ggtggtcagc acgaattcac cgtccatatg ggcgacctgg ctggcttgcg tgtgctccag
     6001 cacctcgatg ccctcggcac ggaaagcggc tgtcaccgcc tcgccgatgg ccgggtcttc
     6061 acggaagaac aaggtattgc gcgccaggac cgtgaccttg ctgcccagcc gggcaaaggc
     6121 ttgcgccagc tccagcgcca ccaccgacga gccgattacg gcaaggcgtt cgggaatggt
     6181 gtcgctcgcc agggcctcgg tggaagtcca gtagggtgac tctttcaagc ccggaatcgg
     6241 cgggaccgcc gggctggcac ccgtggcgac caggcagcgg tcgaacatca cgacgcgctc
     6301 gccaccctcg ttcaaacgga cggtaaggct ctggtcgtcc ttgaagcgcg cctcaccgtg
     6361 cacaacggtg atggccggat taccgcccag gatgccttcg tacttggcgt gccgcagttc
     6421 gtcgacgcgg gcctgctgct gggccagcag cttactgcgg tcaatcgtag gcacagttgc
     6481 cgcaataccg ccatcgaacg ggctttcccg gcgcagatgg gcgatgtggg cggcgcggat
     6541 catgatcttg gacggcacac agccgacatt gacgcaggtg ccgccgatgg tgccgcgctc
     6601 gatcagcgtg acctgcgcgc cttgctcgac ggccttcagc gccgccgcca tcgcggctcc
     6661 accgctgcca atgaccgcta cctgcaccgg gggctcgttg ccactgtgct tttcggcggc
     6721 ggccatccat ccccgcacct tgtcgagcag tccgacgcgg ttgtccgcca gtggcgcatc
     6781 ggctagcgtt gccttgtagc ccagtccggc cacggcggca gtcagcgcgt ccggcgatgt
     6841 gcccggcacg atggcgagtt gcgctgtgcc cttcggatag gacaccagcg ccgactgcac
     6901 gcctggcact ttttccagcg cttccttgac gtgcgccgcg cacgagtcgc aagtcatgcc
     6961 ggtgattttt agatgggtca tgcaacagat cctttatcgt ttgtggcgcc agacaatgac
     7021 gtccgttgtg ctgcggtgcc gttttcagtg actcactgct tgacgctgga cggatagccc
     7081 gcgtctgcgg ttgccttggt cagcttctgc acgctggtct tggcatcgtc gaaggtgacg
     7141 accgcttggc gtgtctcgaa agtcacgtca actttgctga cgccttcgac cttggaaatc
     7201 gccttcttga cagtgatcgg gcaggcggag caggtcatgc ccggtacgga cagcgtgacg
     7261 gtctgggtgg cggcccagac ggnggcaaca acggcggcga gggcgaggga ggcaaacagt
     7321 ntcttcatgg tgaactccga tcagtagana aatggcatga cgtagggaaa tccgagcgcg
     7381 accagaacca gcgcggccac gatccagaaa atgagcttgt aagtagctcg cacttgggga
     7441 atcgcgcaga cctcacccgg tttgcaggcc gctgcctgcc ggtagatgcg ccgccaggcg
     7501 aagaacaacg ccaccagcgc cacgccgata aagatggggc gatagggatc caacaccgcc
     7561 aagttgccga tccaagcgcc gctgaacccc aaggcgatca gaaccaacgg cccgaggcag
     7621 caagccgagg cgaggatggc ggcaagccct ccagtgaaga gcgcgccgcg cccggttttt
     7681 ggttcagaca tgcgcttgtc ctttcgaatt gaaattggat agcgtaacct tacttccgta
     7741 ctcatgtacg gagtcaagcg atatggaaaa caatttggag aacctgacca ttggcgtttt
     7801 cgccaaggcg gccggggtca atgtggagac catccgtttc tatcagcgca agggcttgtt
     7861 gctggagcct gacaagccct atggcagcat ccgccgctat ggcgaggcgg atgtaacgcg
     7921 ggtgcgcttc gtgaaatcag cccagcggct gggcttcagc ctggatgaga tcgccgagct
     7981 gctgcggctg gaggatggca cccattgcga ggaagccagc agtctggccg agcacaagct
     8041 caaggacgtg cgcgagaaaa tggctgacct ggcgcgcatg gaggccgtgc tgtctgagtt
     8101 ggtgtgcgcc tgccatgcgc gaagggggaa cgtttcctgc ccgctgatcg cgtcactaca
     8161 gggtggagca agcttggcag gttcggctat gccttagcgt gctttatttt ccgttttctg
     8221 agacgacccc
//
