LOCUS       Tn6344                  7351 bp    DNA     linear       29-SEP-2020
DEFINITION  Klebsiella michiganensis E718.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Klebsiella michiganensis E718
  ORGANISM  Klebsiella michiganensis E718
            Unclassified.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn6344
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7351)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Klebsiella michiganensis E718"
                     /mol_type="genomic DNA"
     repeat_region   complement(1..16)
                     /gene="IRR_IS5"
     CDS             complement(147..1127)
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS5 transposase"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRRFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRAKVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
     repeat_region   complement(1180..1195)
                     /gene="IRL_IS5"
     repeat_region   1277..1293
                     /gene="IRL_ISEc33"
     CDS             1378..2409
                     /gene="tnpA"
                     /codon_start=1
                     /product="ISEc33 transposase"
                     /translation="MPIIVPIPRGERRLMQKAIHKTRDKNHARRLTAMLMLHRGERVS
                     DVARTLCCARSSVGRWINWFTHSGIEGLKSLPAGRSRRWPFEHICTLLRELIKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLHAGTVRRWLPSAGLVWRRAAPTLRIRDPHKDEK
                     MAVIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFIALLKHLKATYRRAKTITLIVDNYIIHKSRETQRWLK
                     ANPKFRVIYQPVYSPWVNHVEQLWQALHDTITRNHQCRSMWQLLKKVRHFMETASPFP
                     GGKHGQAKV"
     repeat_region   2413..2429
                     /gene="IRR_ISEc33"
     CDS             2686..2937
                     /gene="orf252"
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MQKSTLFNYLRQGKITSACNQFPRWGIRRRKNPSRPGDSSRSGK
                     AALSGWCEMNLICFVIAAFLALDGNDAWPWFLAVGIIMS"
     CDS             2934..3119
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MSRVTAIISAVVMLNGERVDDDRLQLDTNPLLMDAISAVKYLAG
                     KMEQLQNIIAELKASNA"
     CDS             3448..4551
                     /gene="ompC"
                     /codon_start=1
                     /product="Outer membrane protein C"
                     /translation="MKVKVLSLLVPALLVAGAANAAEIYNKDGNKLDLYGKIDGLHYF
                     SDDKSVDGDQTYMRIGVKGETQINDQLTGYGQWEYNVQANNVEGSDKQSWTRLAFAGL
                     KFGDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRANGVATYRNSDFFG
                     LVDGLNFALQYQGKNGSVSGEGQTNNGRGWNKQNGDGFGTAITYDIWEGISAGFAYSH
                     SKRTDDQNAAGLVGRGKYAETYTGGLKYDANNIYLAAEYTQTYNATRFSGDSVSGYAN
                     KAQNFEVVAQYQFDFGLRPSVAYLQSKGKDIEGFGDQDLLKYVDVAATYYFNKNMSAY
                     VDYKINLVDDNRFTSAAGISTDDIVALGLVYQF"
     CDS             4610..5323
                     /codon_start=1
                     /product="RepFIB associated resolvase"
                     /translation="MMDNKPLNKKELESIFLTSGAFNPKGLYLGEGFRKQALELGFPP
                     YILPTEINIVLHYSPELSVRTFVSTLWNTGASLTEVIHLERKSFVFKSPRPYVKVLSS
                     ETESLSLDNDNRLSPRRKIPLLSPYYIAQVKLLIATLKIAGNTCGDSTKPRRIWDVDN
                     FIATSWIDNAIKHARLDGVIFPVEVTPDAIKHAFAMNMLILGVEPSIVMKLMGFKRNK
                     WLEIYLKVKCLIMSLERDM"
     CDS             complement(5533..6081)
                     /gene="apnZ"
                     /codon_start=1
                     /product="Aquaporin Z"
                     /translation="MAYAVGHISGGHFNPAVTLGLWAGGRFPAKDVIGYIIAQVVGGI
                     IAAAILYVVASGKAGFDATASGFASNGYGEHSPGGFSMLSAIVIEIVLTCGFLLVIHG
                     STDKSAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALQQLWLFW
                     VMPIIGGILGGLIYRTLLSSDK"
     repeat_region   complement(6157..6172)
                     /gene="IRR_IS5"
     CDS             complement(6303..7283)
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS5 transposase"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRRFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRAKVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
     repeat_region   complement(7336..7351)
                     /gene="IRL_IS5"
ORIGIN      
        1 ggaaggtgcg aataagcggg gaaattcttc tcggctgact cagtcatttc atttcttcat
       61 gtttgagccg attttttctc ccgtaaatgc cttgaatcag cctatttaga ccgtttcttc
      121 gccatttaag gcgttatccc cagtttttag tgagatctct cccactgacg tatcatttgg
      181 tccgcccgaa acaggttggc cagcgtgaat aacatcgcca gttggttatc gtttttcagc
      241 aaccccttgt atctggcttt cacgaagccg aactgtcgct tgatgatgcg aaatgggtgc
      301 tccaccttgg cccggatgct ggctttcatg tattcgatgt tgatggccgt tttgttcttg
      361 cgtggatgct gtttcaaggt tcttaccttg ccggggcgct cggcgatcag ccagtccaca
      421 tccacctcgg ccagctcctc gcgctgtggc gccccttggt agccggcatc ggctgagaca
      481 aattgctcct ctccatgcag cagattaccc agctgattga ggtcatgctc gttggccgcg
      541 gtggtgacca ggctgtgggt caggccactc ttggcatcga caccaatgtg ggccttcatg
      601 ccaaagtgcc actgattgcc tttcttggtc tgatgcatct ccggatcgcg ttgctgctct
      661 ttgttcttgg tcgagctggg tgcctcaatg atggtggcat cgaccaaggt gccttgagtc
      721 atcatgacgc ctgcttcggc cagccagcga ttgatggtct tgaacaattg gcgggccagt
      781 tgatgctgct ccagcaggtg gcggaaattc atgatggtgg tgcggtcagg caaggcgcta
      841 tccagggata accgggcaaa ccgacgcatg gaggcgattt cgtacagagc atcttccatc
      901 gcgccatcgc tcaggttgta ccaatgctgc atgcagtgaa tgcgtagcat ggtttccagc
      961 ggataaggtc gccggccatt accagccttg gggtaaaacg gctcgatgac ttccaccatg
     1021 ttttgccatg gcagaatctg ctccatacgg gacaagaaaa tctcttttct ggtctgacgg
     1081 cgcttactgc tgaattcact gtcggcgaag gtaagttgat gactcatgat gaaccctgtt
     1141 ccatggctcc agatgacaaa catgatctca tatcagggac ttgttcgcac cttcctaagc
     1201 gtcggcatgg ttctagcgat catatgtcac cgtcagaata tgaaaaccag tattatcaac
     1261 ggctcggaag tgtctaaata gctgcgcgga atagtagatc actgaaaggg aactcagccc
     1321 ggattgtgcg atctgatcaa tcgccaaacc aaccaaaatc accaaccgga ctgagcgatg
     1381 ccgatcatag taccaatacc ccgtggcgaa cgacgcctga tgcagaaagc tattcataaa
     1441 acgcgtgata aaaatcatgc ccgcagactc acggccatgc tgatgcttca tcggggtgaa
     1501 cgggtcagcg atgttgccag aactctctgt tgtgcccgtt catccgttgg tcgctggatt
     1561 aactggttta cgcactcagg tattgaaggc ctgaaatcct tacccgcagg gcgctcccga
     1621 cgctggcctt ttgaacatat ctgcaccctg ttacgtgagc tgataaagca ttctcccggc
     1681 gattttggtt atcaacgttc acgctggagc accgaattac tggcaataaa aatcaatgag
     1741 ataaccggtt gccagttaca tgcaggaacc gttcgccgct ggttgccatc tgcggggctt
     1801 gtatggcgca gggccgcgcc aactctgcgt atccgtgacc cacataaaga tgaaaagatg
     1861 gcggtaatcc acaaagcgct ggatgaatgc agcgcagagc atccggtatt ttatgaagat
     1921 gaagtggata tccaccttaa tcctaaaatc ggtgcggact ggcagttgcg cggacagcag
     1981 aaacgggtag tgacgccggg gcagaacgaa aaatactatc tggccggcgc actgcacagt
     2041 ggcacgggta aagtcagcta cgtgggcggc aacagcaaaa gttcagcgct gtttatcgct
     2101 ctgctgaagc acctgaaagc cacttatcgg cgggcgaaaa caatcacgct gatcgttgat
     2161 aactacatta tccataaaag ccgcgaaaca cagcgctggt tgaaagcaaa tcccaagttc
     2221 agggtaattt accagccggt ttactcgccg tgggtgaatc atgtggaaca gctatggcag
     2281 gcacttcatg acacgataac ccgtaatcat cagtgccgct caatgtggca gttactgaaa
     2341 aaggtccgcc attttatgga aaccgccagc ccattccccg gaggaaaaca tggtcaggca
     2401 aaagtgtagc ggtattaggc gcagctattt agattatccg tggcgattca ggctttttta
     2461 tgtccgttac tattagcata atcactacct acatcagggc tgtgccttaa cacgcatgac
     2521 gttaaaggcg gtgtgtgcaa aactgattag cagatagccg cagaccggga gcgaaacatc
     2581 cttgcggctg agcgctgcgt caatacctac ggcaatggac gacaactaag cgacaataca
     2641 ttttctgctg taacgtcgat cacgtttaac gctggttgcg ccagtatgca gaaatcaact
     2701 ctgtttaatt acctgcgaca gggaaagata actagtgcct gcaaccagtt cccacgctgg
     2761 ggtatacgac ggaggaaaaa tccttcccgg cctggtgact cgtcgcgcag cggaaaagca
     2821 gctctgtctg gatggtgtga aatgaatctg atttgcttcg tcattgctgc gtttcttgcc
     2881 ttagatggta atgatgcgtg gccgtggttt ctggctgttg gaataatcat gtcatgagcc
     2941 gcgtgaccgc cattatcagc gctgtggtta tgctcaacgg cgagagagtg gacgatgatc
     3001 gcttgcaact cgataccaat ccgctgctga tggatgcgat cagcgcggtg aaatatttag
     3061 ctggcaaaat ggagcaattg caaaacatca ttgcagagct gaaggcatca aatgcatgat
     3121 tggtatcgct aattttattc tccagacatc cacatatcag cctcttcgaa catctccagg
     3181 ataactctgc caatttgttc tttttcatgc ttactggcat ctgtatagcc cctcagggca
     3241 tgtccattcc tattgctatg tgttttggtt atgagacacg agatgacgtg ttatgcattc
     3301 cttttacaat atgttacaaa atgtaggtgt attcacaggg aacttttaac tagcttacag
     3361 catgagaagg ctggattttt ttgtgtatag ataattatac aatgacagcc cgatacgcag
     3421 tggcaaatat aaaacgaggg ttcaaccatg aaagttaaag tactatccct cttggtaccg
     3481 gctctgctgg tagcaggtgc agcaaatgcg gctgaaattt ataacaaaga tggcaacaaa
     3541 ttagacctgt acggtaaaat tgatggcttg cattactttt ctgatgacaa gagcgtcgat
     3601 ggcgatcaga cctacatgcg tatcggcgtg aagggcgaaa cccagattaa tgaccaactg
     3661 actggctacg gtcagtggga atacaacgtt caggcgaaca acgttgaagg ttctgacaag
     3721 cagtcctgga ctcgtctggc atttgctggt ctgaaattcg gcgacgtggg ctctttcgac
     3781 tacggtcgta actacggcgt tgtctacgac gtaacttcct ggactgacgt tctgccagag
     3841 tttggcggtg acacttatgg ctctgacaac ttcatgcagc agcgtgctaa tggtgttgca
     3901 acctatcgta actctgactt cttcggtctg gttgacggcc tgaactttgc gctgcagtac
     3961 cagggtaaaa acggtagtgt aagtggtgaa ggccagacta acaatggtcg cggctggaac
     4021 aaacagaacg gtgatggctt cggaacagcg atcacttatg atatttggga aggcatcagc
     4081 gcaggttttg catactctca ttctaaacga actgatgacc agaatgctgc aggtctggtt
     4141 ggtcgaggta aatacgcaga aacttacact ggtggcttga aatatgacgc gaacaatatt
     4201 tacttggctg cggagtatac ccaaacttat aatgcgactc gtttcagtgg tgattccgtc
     4261 agtggttatg ctaataaagc acaaaacttt gaagtggttg cacagtacca gttcgacttc
     4321 ggtctgcgtc cgtccgtggc atacctgcag tccaaaggta aagacatcga aggtttcggc
     4381 gatcaggatc tgctgaaata tgttgatgta gccgcgacct actacttcaa caaaaacatg
     4441 tctgcttatg ttgattataa aatcaatttg gtggatgaca atcgtttcac cagtgctgct
     4501 ggcatctcta ctgatgacat tgtcgcactt ggtttagtat atcagtttta attatttacc
     4561 aaacaccggc cttggtggcc ggtgtttgtg tcgatatgat ggataagata tgatggataa
     4621 taagccatta aataaaaaag aacttgaaag tattttcctt acttcagggg cttttaatcc
     4681 gaaggggttg tatctggggg aggggtttcg taaacaggcc cttgaattgg gttttcctcc
     4741 atatatttta cccacagaga ttaacattgt tttgcattat tcgcctgagt tgagtgtacg
     4801 aacttttgtt tctactttat ggaatactgg tgcttcatta actgaagtga ttcatttaga
     4861 acggaagtct tttgttttta aatctcctcg cccatatgtt aaagtattaa gttctgaaac
     4921 tgaatcattg tcgttagata acgataatag attgtctccc cgccggaaaa ttcctttact
     4981 ttccccatat tatatagctc aagttaagtt attgattgct actttgaaaa tagcgggtaa
     5041 tacttgcggc gattccacca aacctaggag gatatgggat gtggacaatt tcattgcgac
     5101 atcatggata gataatgcta ttaaacatgc cagattggat ggcgtgattt ttccagtaga
     5161 agtcacgccg gatgcaatta aacacgcatt tgcaatgaat atgctgatct tgggagttga
     5221 gccatcaata gtaatgaagt taatggggtt taaaaggaat aagtggcttg aaatatattt
     5281 gaaagttaaa tgcttaatta tgtccttgga gagggatatg tgataaaaag cgatgctttg
     5341 gatcatgttg aggtgctcga aattgcgaca cactcgatca actatacgag gctggataat
     5401 tgtacggttt aaccccccac tccccaccca tacagttgta tgcaaggcct gcagagtggg
     5461 gaggactgaa gattttttcg atggctggcg tgaatatgga atgatctgca gtcgtataga
     5521 gtttttaatt attcatttat cactgctgag caaagtgcga taaattaaac cgcccagtat
     5581 cccaccaatg attggcatga cccagaataa ccataattgt tgcaatgccc aaccgccctg
     5641 aaaaatcgct accgcagtgc tacgcgccgg gtttaccgac gtgttggtga ccggaatgct
     5701 gatcaggtga ataagcgtca gtgccagtcc aatggcgata ggggcgaagc cggcaggagc
     5761 gcttttatcc gtggaaccgt gaataaccag caggaagcca catgtcagga caatctctat
     5821 cacaatggcg gaaagcattg agaagccgcc cggagagtgt tcgccataac cgttcgaagc
     5881 gaacccgcta gcggtggcgt cgaagcccgc tttaccgctg gcgacaacat acagaatagc
     5941 ggcggcgata ataccgccga cgacttgggc gataatataa ccaattacgt ctttggccgg
     6001 gaaacgacct ccagcccata aacctaaagt cactgccggg ttaaaatgtc cgccggaaat
     6061 atgaccgacg gcatatgcca tggttaatac cgttaaaccg aaggccagcg ccaccccagc
     6121 aaaaccaatt cctaatcccg ggaatgctgc cgcaagggaa ggtgcgaata agcggggaaa
     6181 ttcttctcgg ctgactcagt catttcattt cttcatgttt gagccgattt tttctcccgt
     6241 aaatgccttg aatcagccta tttagaccgt ttcttcgcca tttaaggcgt tatccccagt
     6301 ttttagtgag atctctccca ctgacgtatc atttggtccg cccgaaacag gttggccagc
     6361 gtgaataaca tcgccagttg gttatcgttt ttcagcaacc ccttgtatct ggctttcacg
     6421 aagccgaact gtcgcttgat gatgcgaaat gggtgctcca ccttggcccg gatgctggct
     6481 ttcatgtatt cgatgttgat ggccgttttg ttcttgcgtg gatgctgttt caaggttctt
     6541 accttgccgg ggcgctcggc gatcagccag tccacatcca cctcggccag ctcctcgcgc
     6601 tgtggcgccc cttggtagcc ggcatcggct gagacaaatt gctcctctcc atgcagcaga
     6661 ttacccagct gattgaggtc atgctcgttg gccgcggtgg tgaccaggct gtgggtcagg
     6721 ccactcttgg catcgacacc aatgtgggcc ttcatgccaa agtgccactg attgcctttc
     6781 ttggtctgat gcatctccgg atcgcgttgc tgctctttgt tcttggtcga gctgggtgcc
     6841 tcaatgatgg tggcatcgac caaggtgcct tgagtcatca tgacgcctgc ttcggccagc
     6901 cagcgattga tggtcttgaa caattggcgg gccagttgat gctgctccag caggtggcgg
     6961 aaattcatga tggtggtgcg gtcaggcaag gcgctatcca gggataaccg ggcaaaccga
     7021 cgcatggagg cgatttcgta cagagcatct tccatcgcgc catcgctcag gttgtaccaa
     7081 tgctgcatgc agtgaatgcg tagcatggtt tccagcggat aaggtcgccg gccattacca
     7141 gccttggggt aaaacggctc gatgacttcc accatgtttt gccatggcag aatctgctcc
     7201 atacgggaca agaaaatctc ttttctggtc tgacggcgct tactgctgaa ttcactgtcg
     7261 gcgaaggtaa gttgatgact catgatgaac cctgttccat ggctccagat gacaaacatg
     7321 atctcatatc agggacttgt tcgcaccttc c
//
