LOCUS       Tn5                     5818 bp    DNA     linear   BCT 29-SEP-2020
DEFINITION  Escherichia coli.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Escherichia.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     repeat_region   1..9
                     /gene="IRL_IS50R"
     misc_feature    92..1522
                     /gene="tnpA"
                     /product="IS50R transposase (pseudogene)"
     repeat_region   1525..1533
                     /gene="IRR_IS50R"
     CDS             1551..2345
                     /gene="aphA-2"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2366..2746
                     /gene="ble"
                     /codon_start=1
                     /transl_table=11
                     /product="Bleomycin resistance protein"
                     /translation="MTDQATPNLPSRDFDSTAAFYERLGFGIVFRDAGWMILQRGDLM
                     LEFFAHPGLDPLASWFSCCLRLDDLAEFYRQCKSVGIQETSSGYPRIHAPELQEWGGT
                     MAALVDPDGTLLRLIQNELLAGIS"
     CDS             2743..3585
                     /gene="strB"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside O-phosphotransferase APH(6)-Ic"
                     /translation="MSVSSRFPPEVTAWMERWRLLRDGELLTTHSSWILPVRQGDMPA
                     MLKVARIPDEEAGYRLLTWWDGQGAARVFASAAGALLMERASGAGDLAQIAWSGQDDE
                     ACRILCDTAARLHAPRSGPPPDLHPLQEWFQPLFRLAAEHAALAPAASVARQLLAAPR
                     EVCPLHGDLHHENVLDFGDRGWLAIDPHGLLGERTFDYANIFTNPDLSDPGRPLAILP
                     GRLEARLSIVVATTGFEPERLLRWIIAWTGLSAAWFIGDGDGEGEGAAIDLAVNAMAR
                     RLLD"
     CDS             3711..3923
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MAEGLWKVPDDLPEQGRRYDAQRLGGVTVELKSHLPIERQARVI
                     GATWLDQQLIDGGSGLGDLGFSSEAK"
     CDS             4084..4296
                     /codon_start=1
                     /transl_table=11
                     /product="VirD2 components relaxase"
                     /translation="MADGQRVAGVYRRPVMLASGRNGMLDDAKGSSLVRWKPIEQRLG
                     EQLAATVRGGGVSWEIGRQRGPAPVS"
     repeat_region   complement(4286..4294)
                     /gene="IRR_IS50R"
     CDS             complement(4297..5727)
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS50R transposase"
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGK
                     SITISSEGSEAMQEGAYRFYRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLS
                     YRHQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEK
                     ESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDRLAHNERFVVRSKHPRKDV
                     ESGLYLIDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGN
                     ITLNAVLAEEINPPKGETPLKWLLLTGEPVESLAQALRVIDIYTHRWRIEEFHKAWKT
                     GAGAERQRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQS
                     AETVLTPDECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALW
                     EGWEALQSKLDGFLAAKDLMAQGIKI"
     repeat_region   complement(5810..5818)
                     /gene="IRL_IS50R"
ORIGIN      
        1 ctgactctta tacacaagta gcgtcctgaa cggaaccttt cccgttttcc aggatctgac
       61 ttccatgtga cctcctaaca tggtaacgtt catgataact tctgctcttc atcgtgcggc
      121 cgactgggct aaatctgtgt tctcttcggc ggcgctgggt gatcctcgcc gtactgcccg
      181 cttggttaac gtcgccgccc aattggcaaa atattctggt aaatcaataa ccatctcatc
      241 agagggtagt gaagccatgc aggaaggcgc ttaccgattt taccgcaatc ccaacgtttc
      301 tgccgaggcg atcagaaagg ctggcgccat gcaaacagtc aagttggctc aggagtttcc
      361 cgaactgctg gccattgagg acaccacctc tttgagttat cgccaccagg tcgccgaaga
      421 gcttggcaag ctgggctcta ttcaggataa atcccgcgga tggtgggttc actccgttct
      481 cttgctcgag gccaccacat tccgcaccgt aggattactg catcaggagt ggtggatgcg
      541 cccggatgac cctgccgatg cggatgaaaa ggagagtggc aaatggctgg cagcggccgc
      601 aactagccgg ttacgcatgg gcagcatgat gagcaacgtg attgcggtct gtgaccgcga
      661 agccgatatt catgcttatc tgcaggacag gctggcgcat aacgagcgct tcgtggtgcg
      721 ctccaagcac ccacgcaagg acgtagagtc tgggttgtat ctgatcgacc atctgaagaa
      781 ccaaccggag ttgggtggct atcagatcag cattccgcaa aagggcgtgg tggataaacg
      841 cggtaaacgt aaaaatcgac cagcccgcaa ggcgagcttg agcctgcgca gtgggcgcat
      901 cacgctaaaa caggggaata tcacgctcaa cgcggtgctg gccgaggaga ttaacccgcc
      961 caagggtgag accccgttga aatggttgtt gctgaccggc gaaccggtcg agtcgctagc
     1021 ccaagccttg cgcgtcatcg acatttatac ccatcgctgg cggatcgagg agttccataa
     1081 ggcatggaaa accggagcag gagccgagag gcaacgcatg gaggagccgg ataatctgga
     1141 gcggatggtc tcgatcctct cgtttgttgc ggtcaggctg ttacagctca gagaaagctt
     1201 cacgctgccg caagcactca gggcgcaagg gctgctaaag gaagcggaac acgtagaaag
     1261 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa
     1321 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
     1381 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
     1441 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
     1501 ggcgcagggg atcaagatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     1561 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     1621 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     1681 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     1741 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     1801 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     1861 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     1921 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     1981 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     2041 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     2101 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     2161 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     2221 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     2281 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     2341 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     2401 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     2461 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccgg
     2521 gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt
     2581 ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc agcggctatc cgcgcatcca
     2641 tgcccccgaa ctgcaggagt ggggaggcac gatggccgct ttggtcgacc cggacgggac
     2701 gctcctgcgc ctgatacaga acgaattgct tgcaggcatc tcatgagtgt gtcttcccgt
     2761 tttccgcctg aggtcactgc gtggatggag cgctggcgcc tgctgcgcga cggcgagctg
     2821 ctcaccaccc actcgagctg gatacttccc gtccgccagg gggacatgcc ggcgatgctg
     2881 aaggtcgcgc gcattcccga tgaagaggcc ggttaccgcc tgttgacctg gtgggacggg
     2941 cagggcgccg cccgagtctt cgcctcggcg gcgggcgctc tgctcatgga gcgcgcgtcc
     3001 ggggccgggg accttgcaca gatagcgtgg tccggccagg acgacgaggc ttgcaggatc
     3061 ctctgcgaca ccgccgctcg tctgcacgcg ccgcggtccg gaccgccgcc cgatctccat
     3121 ccgctacagg aatggttcca gccgcttttc cggttggccg ctgagcacgc ggcacttgcg
     3181 cccgccgcca gcgtagcgcg ccaacttctg gcggcgccgc gcgaggtgtg cccgctccac
     3241 ggcgacctgc accacgagaa cgtgctcgac ttcggcgacc gcggctggct ggccatcgac
     3301 ccgcacggac tgctcggcga gcgcaccttc gactatgcca acatcttcac gaatcccgat
     3361 ctcagcgacc ccggtcgccc gcttgcgatc ctgccgggca ggctggaggc tcgactcagc
     3421 attgtggtcg cgacgaccgg gtttgagccc gaacggcttc ttcgctggat cattgcatgg
     3481 acgggcttgt cggcagcctg gttcatcggc gacggcgacg gcgagggcga gggcgctgcg
     3541 attgatctgg ccgtaaacgc catggcacgc cggttgcttg actagcgcgg tcaccgatct
     3601 cacctggtcg tcgagctagg tcaggccgtg tcgggcgtga tccgctggaa gtcgttgcgg
     3661 gccacacccg ccgcctcgaa gccctgcacc aggccggcat cgtggtgtgc gtggccgagg
     3721 gactatggaa ggtgccggac gatctgcccg agcagggccg ccgctatgac gcccagcgtc
     3781 ttggtggcgt gacggtggag ctgaaatcgc acctgcccat cgagcggcag gcccgcgtga
     3841 tcggtgccac ctggcttgac cagcagttga tcgacggtgg ctcgggcttg ggcgacctgg
     3901 gctttagcag tgaggccaag taggcgatac agcagcgcgc ggacttcctg gccgaacagg
     3961 gactggccga gcggcgcggg cagcgcgtga tcctcaccgg aatctgctcg gcagcagcgg
     4021 gctcgggaac tggcgcaggc cgcgaaggac attgccgccg ataccggcct ggagcatcgc
     4081 cccgtggccg acggccagcg cgttgccggc gtctaccggc gccccgtcat gctcgccagc
     4141 gggcgaaatg ggatgcttga tgacgccaag gggtccagcc tcgtgcggtg gaagcccatc
     4201 gaacagcggc ttggggagca gctcgccgcg acggtgcgcg gtggcggcgt gtcttgggag
     4261 attggacgac agcgtgggcc ggcccctgtc tcttgatcag atcttgatcc cctgcgccat
     4321 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttccca
     4381 gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc
     4441 tatcgccatg taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt
     4501 gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct
     4561 ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa
     4621 gctttctctg agctgtaaca gcctgaccgc aacaaacgag aggatcgaga ccatccgctc
     4681 cagattatcc ggctcctcca tgcgttgcct ctcggctcct gctccggttt tccatgcctt
     4741 atggaactcc tcgatccgcc agcgatgggt ataaatgtcg atgacgcgca aggcttgggc
     4801 tagcgactcg accggttcgc cggtcagcaa caaccatttc aacggggtct cacccttggg
     4861 cgggttaatc tcctcggcca gcaccgcgtt gagcgtgata ttcccctgtt ttagcgtgat
     4921 gcgcccactg cgcaggctca agctcgcctt gcgggctggt cgatttttac gtttaccgcg
     4981 tttatccacc acgccctttt gcggaatgct gatctgatag ccacccaact ccggttggtt
     5041 cttcagatgg tcgatcagat acaacccaga ctctacgtcc ttgcgtgggt gcttggagcg
     5101 caccacgaag cgctcgttat gcgccagcct gtcctgcaga taagcatgaa tatcggcttc
     5161 gcggtcacag accgcaatca cgttgctcat catgctgccc atgcgtaacc ggctagttgc
     5221 ggccgctgcc agccatttgc cactctcctt ttcatccgca tcggcagggt catccgggcg
     5281 catccaccac tcctgatgca gtaatcctac ggtgcggaat gtggtggcct cgagcaagag
     5341 aacggagtga acccaccatc cgcgggattt atcctgaata gagcccagct tgccaagctc
     5401 ttcggcgacc tggtggcgat aactcaaaga ggtggtgtcc tcaatggcca gcagttcggg
     5461 aaactcctga gccaacttga ctgtttgcat ggcgccagcc tttctgatcg cctcggcaga
     5521 aacgttggga ttgcggtaaa atcggtaagc gccttcctgc atggcttcac taccctctga
     5581 tgagatggtt attgatttac cagaatattt tgccaattgg gcggcgacgt taaccaagcg
     5641 ggcagtacgg cgaggatcac ccagcgccgc cgaagagaac acagatttag cccagtcggc
     5701 cgcacgatga agagcagaag ttatcatgaa cgttaccatg ttaggaggtc acatggaagt
     5761 cagatcctgg aaaacgggaa aggttccgtt caggacgcta cttgtgtata agagtcag
//
