LOCUS       Tn5563                  6253 bp    DNA     linear       29-SEP-2020
DEFINITION  Pseudomonas alcaligenes.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Pseudomonas alcaligenes
  ORGANISM  Pseudomonas alcaligenes
            Unclassified.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn5563
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Pseudomonas alcaligenes"
                     /mol_type="genomic DNA"
     repeat_region   1..42
                     /gene="IRL_Tn5563"
     CDS             complement(41..970)
                     /gene="tnpR"
                     /codon_start=1
                     /product="Tn5563 resolvase"
                     /translation="MKIGYARVSTRDQKADLQVDALKQAGCERIYQDIASGAKSARPE
                     LDKLLANVRPGDAVVIWKLDRLGRSLKHLVELVGELAERKVGLQSLNDPIDTTHAQGR
                     LVFNLFASLAEFERELIRERTQAGLSAARARGRIGGRPKGLPAKAEATAMAAETLYRE
                     GRLSVSAIGEKLHISKSTLYSYLRHRGVEIGAYQKSARSRDQQPSAASPAEPPAAERV
                     ATVTLRLAVVNNSKFVRGRKRATENIERYCLEPYGMKRLDAGHYELTIPYRSDDELDK
                     SVHDLLTEISQEADMRNCFVEMGAWEEDTEKRW"
     misc_recomb     981..1110
                     /gene="res"
     CDS             1172..1411
                     /gene="orf237"
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MNTIRWNVAVSADTDQSLRMFLASQGGGRKGDLSRFIEEAVRAH
                     ILELSAEQAKAANAHLSEAELTNAVDEALDWARKR"
     CDS             1411..1821
                     /gene="orf408"
                     /codon_start=1
                     /product="PilT domain-containing protein"
                     /translation="MRVVLDTNILFSALISPHGAPDAIYRAWRAARFEVVTSRMQLDE
                     IRRASRYPKLQAILQPAKVGAMINNLQRAVVLERLTIEVEADDPDDSFLLAMALAGDA
                     DYLVTGDRRAGLLQRGHIERTRIVTPAVFCVEVL"
     CDS             1825..4818
                     /gene="tnpA"
                     /codon_start=1
                     /product="Tn5563 transposase"
                     /translation="MPVGFLTQEQRDGFGRYVDSPSREELERYFHLSDEDREAIQVLR
                     GNHNRLGYAVLLTTVRFVGVLPDKPAAVPVEVLQVLCRQLAIPDPDCLQRYSDHRRWI
                     HATDIQNRFGYRHFTDPGIGFRLSRWLYALCWTGTDRPGVLFERATSWLFTQKVLLPG
                     VSQLERFIAQLRSRVEERLWFTLGRSVTEEQRLQLQDLLTVAEGNRSSRLDQLRSGPV
                     MVSGPALIRALRRLDDVRGIGITLPAAAHIPPSRIAALARFANTAKVTAINRLPASRR
                     MATLVAFALCLEATAHDDALEVLEALLRDLFSNAEKADKKARMRSLKDLDRSAATLAA
                     ACKVVLDSSISDDNVRARLFNDLPRTTLEKALEEVNALIRPVDDVYFLALEARYRSVR
                     RFLPDLLKHIRFGFSPAGKGVAASLEWLQLNLPRRKPEDDAPQEIVAKAWQKHITRED
                     GSLDMGAYVFCTLDALRTALRRRDVFVSPSWRYADPRLGLLDGAEWLAARPIICRSLG
                     LTIDAKTTLDALSVELDATWLAVAARLPDNPAIQLSENTEGKTELSLGALDKLDEPCS
                     LLQLRAAVSDLMPRVDLPEILLEIAARTGFSEAFTHVSERNARADNLVTSLCAVLLGG
                     ACNTGLEPLIRTDNPALRRDRLSWVSQNYIRDDTLSAANAILVGAQSQLELAQVWGGG
                     EVASADGMRFVVPVRTVHAGPNPKYFGTGRGVTWYNLISDQFSGLNAITVPGTLRDSL
                     VLLAVVLEQQTELQPTQIMTDTGAYSDVVFGLFRLLGYHFSPRLADVGGTRFWRTRPD
                     ADYGKLNGLARQSVKLDLIAEHWDDLLRLAGSLKLGRVPATGIMRTLQTGDRPTRLAQ
                     ALAEFGRIEKTLHTLTYIDDESKRRATLTQLNRGEGRHSLARAVFHGKRGELRQRYRE
                     GQEDQLGALGLVVNIIVLWNTLYMTAAVERLKQHGYPVLEEDLARLSPLIYEHINMLG
                     RYSFAVPEEVARGELRPLRNPDDDL"
     CDS             complement(5051..5326)
                     /gene="merP"
                     /codon_start=1
                     /product="Mercuric transport protein periplasmic
                     component"
                     /translation="MRKLLIAVLFALPFVALAAPPKTVTLDVQNMTCGLCPITVKKSL
                     EKVSGVSDVQVNFDQKTATVTYDPDKAQPEALTEATANAGYPSTVQK"
     CDS             complement(5339..5695)
                     /gene="merT"
                     /codon_start=1
                     /product="Mercuric transport protein"
                     /translation="MGMGMQLTGKGSLVASSLTAIGASVCCVGPLVLLALGVGGTWVG
                     ALTMMEPLRPLFIGLTLLFLGLAFRKLYLVPQVCTPGTPCADPRTLVRQRLVFWIVSV
                     LLLGLLAVPWLAPLFY"
     CDS             5764..6162
                     /gene="merR"
                     /codon_start=1
                     /product="Mercuric resistance operon regulatory protein"
                     /translation="MATELTIGKLADAAGVNVETIRYYQRRGLLDEPAKPLGGHRRYP
                     VDMVKRLRFIKRAQALGFTLSEVGGLLTLDESCACAETRARAARKLALIEQKMADLVV
                     MQQLLGELVQQCDAGDGGTICPIIEALIRE"
     repeat_region   6212..6253
                     /gene="IRR_Tn5563"
ORIGIN      
        1 ggggtttggg gagcaatgga accaaaaacc aacgtaagcc ctaccaacgc ttttcggtgt
       61 cttcttccca ggcgcccatc tcgacaaaac agttgcgcat gtcggcctcc tggctgatct
      121 cggtcagcag gtcatgcacg ctcttgtcca gctcatcgtc gctccgatac ggaatggtca
      181 actcatagtg gccggcatcc agccgcttca tgccataggg ctccaggcag tagcgctcaa
      241 tgttctccgt ggcccgcttc cggccgcgca cgaacttgct gttattcacc accgcgaggc
      301 gcagggtgac ggtggccacc cgctcggcgg cgggcggctc tgccggcgac gcggccgaag
      361 gctgctggtc gcgtgacctg gcgctcttct ggtacgcgcc gatctcgaca ccacggtggc
      421 gcaggtagct gtacagcgtg ctcttggaga tgtgcagctt ctcgccgatc gcgctgacgc
      481 tcaggcgacc ttcgcggtag agggtttcgg ccgccatggc ggtggcctca gccttggctg
      541 gcaggccctt gggacggcca ccgatccggc cacgcgcccg tgcggccgac agacccgcct
      601 gagtccgctc gcggatcagc tcgcgctcga actccgccag cgaggcgaac aggttgaaca
      661 ccaggcggcc ttgggcgtgg gtggtgtcga tggggtcatt caggctctgt aagccgacct
      721 tgcgctctgc cagctcgccg accaactcga ccaggtgctt gagggaacgc ccaaggcgat
      781 ccagcttcca gatcaccacg gcatcacccg gccgcacgtt ggccagcagt ttgtccaact
      841 ccggccgggc gcttttcgcg ccgctggcga tgtcttggta gatgcgttcg cacccggcct
      901 gtttcagggc atcgacttgt aggtcggctt tctgatcccg agtgctcact cgcgcataac
      961 cgatcttcat caaaagtact gtttactcga ctacgttagt aatagttgaa ctttgattaa
     1021 gcgtaccagt tatttgaacc gtagcgcggg gagcttaacg aaccgagcca ttcctcgata
     1081 gagttcggca aaaccttcgt tttgtcgaac cattgcatcg ccctttgtga ttggcgtata
     1141 aacacacaaa cacctattag cggagaacgc tatgaatacc attcgctgga atgtcgccgt
     1201 ctcggccgac accgaccagt cgcttcggat gtttctggcc agccagggcg gcggccgtaa
     1261 gggcgacctg tcgcgcttca tcgaagaagc ggtacgggca cacatcctgg agctgagcgc
     1321 tgagcaggcc aaggccgcta acgcccatct gagtgaggca gaattgacca acgcggttga
     1381 cgaagcgctc gactgggcac gtaagcgctg atgcgggtcg tgttggatac caacatcctg
     1441 ttcagcgccc tgatctcgcc acatggcgcg cccgatgcga tctaccgtgc ctggcgggcg
     1501 gcgcgtttcg aggtggtgac ctcgcggatg caactcgatg aaattcgtcg agccagccgc
     1561 tatcccaagc ttcaggccat cctacagccc gccaaggtgg gcgccatgat caataacctg
     1621 caacgggctg tggtactgga gcgcctgacc atcgaggtcg aagccgatga tccggatgac
     1681 tcgtttttgc tggccatggc cttggcgggc gatgcggact acctggtaac cggtgatcgc
     1741 cgcgccggcc tgctgcaacg cgggcacatc gaacgcacgc ggatcgtcac gcccgccgtg
     1801 ttctgcgtcg aggtgctgtg atcaatgccg gtcggttttc tgactcaaga gcaacgcgac
     1861 ggttttggcc gctatgttga ttcgcccagc cgtgaagagc tggaacgtta cttccacctg
     1921 agcgatgaag accgtgaagc catccaggtg ctgcggggta accataaccg tctgggttat
     1981 gccgttctgc tgaccaccgt ccgcttcgtt ggcgttctgc cggacaagcc cgccgccgtg
     2041 ccggtggaag tcctgcaggt gctttgccga caactggcga ttccagaccc cgactgcctc
     2101 cagcgctata gcgatcatcg ccgctggata catgccaccg atattcagaa ccgctttggc
     2161 tatcgtcatt tcaccgatcc gggcatcggc tttcgcttga gccgctggct gtatgccctc
     2221 tgctggacgg gcaccgaccg gccgggagtg ctgtttgagc gagccacctc gtggctgttc
     2281 acacagaaag tcctcctgcc tggtgtgtct caactagagc gctttatcgc ccagttgcgc
     2341 agtcgggtcg aagaacgcct ctggtttacg ctgggccgca gcgtgactga ggaacagcga
     2401 ttgcaactgc aagacttgct gacggtggcc gaaggcaacc gcagctcccg gctggatcaa
     2461 ttgcgctccg gcccggtcat ggtcagtggc cccgcgttga ttcgggcact gcgccggctc
     2521 gatgacgtgc gcggcatcgg catcaccttg ccggcggcgg cgcacatccc tcccagccgt
     2581 atcgccgccc tggcccgctt cgccaacacg gccaaggtca ccgcgattaa tcggctgccg
     2641 gcgtcgcggc ggatggcgac actggtggcc ttcgcactct gcctggaggc gactgcgcac
     2701 gacgacgcac tggaagtcct ggaggccttg ctgcgcgacc tgttcagcaa cgcggagaaa
     2761 gccgacaaga aagcccgcat gcgcagcctg aaagacctgg atcggtcggc cgcgacgctc
     2821 gccgccgcgt gcaaggtcgt gctggacagc tcgatcagcg atgacaacgt gcgcgcccgg
     2881 ctgttcaacg acctgccgag gaccaccctg gaaaaggccc tggaagaggt caacgcgctg
     2941 atccgcccgg tagatgacgt ctattttctt gcattggaag cgcgctaccg cagcgtgcgc
     3001 cgcttcctgc ccgacctgct caagcacatc cgcttcggct tcagcccggc cggcaagggc
     3061 gtggcggcta gtctggagtg gctgcaactg aacctgccgc gccggaagcc agaggatgac
     3121 gcgccgcagg agatcgtggc caaggcttgg cagaagcaca tcacccgcga agatggctcc
     3181 ctcgacatgg gtgcctatgt gttctgcacg ctcgatgcgc tgcgcacggc cctgcgccgc
     3241 cgcgatgtct tcgtctcgcc cagttggcgc tatgccgacc cgcgccttgg cctgctcgac
     3301 ggtgccgaat ggctggcggc gcgaccgatc atctgccggt cactgggcct gaccatcgac
     3361 gccaaaacca ccctggacgc cttgtccgtc gagctggatg caacctggct ggcagtagcc
     3421 gcgcgcctgc ccgacaaccc ggcgattcaa ctgagcgaga acaccgaggg caagaccgaa
     3481 ctgtcgctcg gggcgctgga caagctggac gagccctgct cgttgctgca actgcgggcg
     3541 gccgtgtctg acctgatgcc gcgtgtcgat ctgccggaaa tcctcttgga aatcgccgcc
     3601 cgcactggct tttccgaggc cttcacccat gtctccgaac gcaatgcacg cgccgacaac
     3661 ctggtcacca gcctctgcgc ggtgctgttg ggcggggcct gcaacaccgg cctggagccc
     3721 ttgatccgca ccgacaaccc ggcgctgcgc cgtgaccggc tgtcctgggt cagccagaat
     3781 tatatccgcg acgacaccct gtcagcggct aacgccatcc tggtcggagc gcaaagccaa
     3841 ctggaactgg cccaagtctg gggtggcggc gaggtcgcct ccgccgatgg catgcgcttc
     3901 gtcgtaccgg tgcgcaccgt gcatgccggc cccaatccga agtatttcgg caccggccgg
     3961 ggtgtcacct ggtacaacct gatttccgac caattctccg gcctcaacgc catcaccgtg
     4021 cccggcacgc tgcgcgacag cctggtgttg ctggcggtcg tgctggaaca gcagaccgag
     4081 ttgcagccga cgcaaatcat gaccgacacc ggggcctaca gcgatgtggt gttcgggctc
     4141 ttccgcctac ttggctacca cttcagtccg cggctggccg atgtcggcgg tacccgcttc
     4201 tggcgcacgc gcccggacgc ggactacggc aagctcaacg ggctggcccg ccagtcggtc
     4261 aaactcgacc tgatcgccga gcactgggac gacctgctgc gcctggccgg ctcgctcaaa
     4321 ctcggccggg tgccggcgac tggcatcatg cgcacgctgc aaacgggaga tagacccacc
     4381 cggctggccc aggcgctggc cgaattcggg cggatcgaaa agactctgca cacgttgacc
     4441 tatatcgacg acgagtccaa gcgccgcgcc accctgaccc agttgaaccg aggcgaaggc
     4501 cggcacagcc tggcccgcgc cgtgttccac ggcaaacgcg gcgagctccg ccagcgctac
     4561 cgcgaaggcc aggaagacca gctcggtgct ctgggcctgg tggtgaacat catcgtgctg
     4621 tggaacaccc tctacatgac ggcggccgtg gaacggctca agcagcacgg ctatccagtg
     4681 ctggaagagg atttggcccg gctatcgccg ctgatctacg agcacatcaa catgctcggg
     4741 cggtattcct ttgcggtacc ggaagaagtt gcgcgcggcg agctgcggcc actgcgtaat
     4801 ccagacgacg acctgtgatc cccctaaacg ctatgagcaa cacccaagct gctgctggat
     4861 cggcgggcac ttcaccgagc cgaacgaaca gaaaacgcag caatcccctg ggttggggcg
     4921 cagcagcgcc ttgcagttgc tgcactcgta atagaactgg caggcgtccg tgggcatggt
     4981 ttccggcttg gcgaagccgc agcgcgggca agtcagcacg gactcaagga caatggcgct
     5041 catcgtgacc tcacttctgc actgtggagg ggtatcccgc gttcgcggtc gcctcagtca
     5101 gtgcctcggg ctgggcctta tcgggatcgt aggtgacggt cgccgtcttc tggtcgaaat
     5161 tgacctggac gtcactcacg ccggacacct tctccagcga cttcttgacc gtgatcggac
     5221 agagtccgca cgtcatgttc tgcacgtcga gcgtgacggt tttcggggga gccgccagcg
     5281 ccacgaaggg caaggcgaaa agcacggcga tcagcagttt gcgcatggtt aatctccttc
     5341 agtagaacag cggggcgagc cacggcacgg ccaataggcc gagcagcagc acgctgacga
     5401 tccagaacac gagtcgctgt cgcacgagcg tgcgcggatc ggcgcagggt gtacctggcg
     5461 tacaaacctg tggcaccagg tagagcttgc ggaatgccaa tcccaggaac agtagagtca
     5521 acccgatgaa gagggggcgt agtggctcca tcatggtcag agcgcccacc cacgtaccgc
     5581 cgacaccgag tgccaacagc accagtggcc cgacacagca caccgacgca ccgatggcgg
     5641 tcagcgaact cgcgaccagc gagcctttcc cggtgagttg catccccatt cccatctcct
     5701 gagcctgatt gcctaggtgc tattctaaac tccgtaccaa agtacggaat caaggagaaa
     5761 gtgatggcca cagagctgac cattggcaag ctggcagacg ccgccggggt gaacgtcgag
     5821 acgatccgct actaccagcg acgcgggctg ctggatgaac cagccaaacc cttgggtggc
     5881 catcggcgct atccggtgga catggtgaag cgactgcgtt tcatcaagcg ggctcaggcg
     5941 ctgggtttca cgctctcgga agtcggtgga ctgctgacgc tggatgagtc gtgcgcctgt
     6001 gccgaaacgc gagcacgggc tgcacgcaag ctcgcgttga tcgagcagaa gatggccgac
     6061 ttggtcgtca tgcagcaact gttaggcgaa ctggtgcagc aatgtgatgc gggagacggc
     6121 ggaacgatct gcccgatcat cgaggcactg atcagagagt aatgcctggc gggtgagctg
     6181 gagatcggaa agccccttta cggtgggatt cagagcttac gttggttttt ggttccattg
     6241 ctccccaaac ccc
//
