LOCUS       Tn5058a                12373 bp    DNA     linear       27-NOV-2020
DEFINITION  Pseudomonas sp. ED23-33.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Pseudomonas sp. ED23-33
  ORGANISM  Pseudomonas sp. ED23-33
            Unclassified.
REFERENCE   1  (bases 1 to 12373)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn5058a
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12373)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12373
                     /organism="Pseudomonas sp. ED23-33"
                     /mol_type="genomic DNA"
     mobile_element  1..12373
                     /product="Tn5058a"
                     /note="Unit transposon: Tn5058a"
     repeat_region   1..25
                     /product="IRL_Tn5058a"
                     /note="Tn5058a inverted repeat left"
     repeat_region   9..27
                     /product="TniA-binding site 1"
     repeat_region   49..67
                     /product="TniA-binding site 2"
     repeat_region   78..96
                     /product="TniA-binding site 3"
     repeat_region   110..128
                     /product="TniA-binding site 4"
     CDS             144..1826
                     /note="Tn5058a transposase"
                     /codon_start=1
                     /transl_table=11
                     /product="tniA"
                     /translation="MASDTSLIAEQGVATLPDAAWAQARQRAEIIGPLAALDVVGHEA
                     ADAAAHALGLSRRQVYVLIRRARQGAGLVTDLARSRSGGGKGKGRLPESVERIIRELL
                     QKRFLTKQKRSLAAFHREVAQACKAQKLRAPARNTVALRIAGLDPLKATRRREGQDAS
                     RSLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDERDRQPIGRPYLTIAIDVFTRCVLG
                     MVVTLEAPSSVSVGLCLVHVACDKRPWLEGLKIEMEWPMSGKPRLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAIARTGVPTVITRTTAFLV
                     DFLPIIRRTLTRTGFVIDHIHYYADALKPWIARRDRLPAFLIRRDPRDISRIWVLEPE
                     GQHYLEIPYRTLSHPAVTLWEQRQALAKLRQQGREQVDESALFRMIGQMREIVSTAQK
                     ATRKARRDADRRQHLKATAVLFKTTPPPDADMADPQADNQPPAKPFDQIEEW"
     CDS             1829..2737
                     /note="Tn5058a transposition regulator"
                     /codon_start=1
                     /transl_table=11
                     /product="tniB"
                     /translation="MEEYPIIDLSHLMPVAQGLARLPADERIHRLRADRWIGYPRAVE
                     ALNRLEALYAWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQ
                     MPSEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAG
                     NSVNRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDD
                     CCSLLASFAASLPLRRPSSIATLDMARYLLTRSEGTIGELAHLLMAAAVAAVESGEEA
                     INHRTLSMADYTGPSERRRQFERELM"
     CDS             2734..3951
                     /note="Tn5058a target-site selection protein"
                     /codon_start=1
                     /transl_table=11
                     /product="tniQ"
                     /translation="MKPAPHWPLHPAPREGEALSSWLNRVALCYHMEVSELLEHDLGH
                     GQVDDLDTAPPLALLAMLSQRSGIEPDRLRCMSFAGWVPWLLDSLDDQIPDALETYAF
                     QLSVLLPKLRRRTRSITSWRAWLPSQPIHRACPLCLNDPENQAVLLAWKLPLMLSCPL
                     HGCWLESYWGVPGRFLGWENADAEPRTASDAIAAMDQRTWQALTTGHVELPRRRIHAA
                     LWFRLLRTLLDELNTPLSTCGTYAGYLRQIWECCGHPLRAGQSLWRPYETLNPAVRLQ
                     MLEAAATAISLIEVRDISPPGEHAKLFWSEPQTGFTSGLPAKAPKPEPVDHWQRAIQA
                     IDEAIIEARHNPETARSLFALASYGRRDPASLEQLRATFAKEGIPPEFLSHYEPSLPF
                     ACLRQNDGLSDKF"
     misc_recomb     3975..4004
                     /product="res"
                     /note="Resolution site"
     CDS             4012..4626
                     /note="Tn5058a resolvase"
                     /codon_start=1
                     /transl_table=11
                     /product="tniR"
                     /translation="MLIGYMRVSKADGSQATDLQRDALVAAGVDPAHLYEDQASGKRE
                     DRPGLASCLKALRPGDTLVVWKLDRLGRDLRNLINTVHDLTERGIGLKVLTGHVAAID
                     TTTAAGKLVFGIFAALAEFERELIAERTVAGLASAQTRGRKGGRPFKMTAAKLRLAMA
                     AMGQSQTKVGDLCQELGITRQTLYRHISPKGELRPDGEKLLSRI"
     CDS             complement(4665..5006)
                     /note="Urf2 protein"
                     /codon_start=1
                     /transl_table=11
                     /product="urf2"
                     /translation="MSASRPDGWTTAEGPSVRARARLSVRKADAGAAFAVFVSQWRGA
                     TMNANDPTATSCCVCWSKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATETSG
                     EPNACGSPPSN"
     CDS             complement(5003..5239)
                     /note="Mercuric resistance protein MerE"
                     /codon_start=1
                     /transl_table=11
                     /product="merE"
                     /translation="MNSPERIPAETHKPFTGYLWGTLAVLTCPCHLSILAAVLAGTTA
                     GAFLVEYWVITALGLTGLFLLSLARALRAFRERS"
     CDS             complement(5236..5601)
                     /note="Mercuric resistance protein MerD"
                     /codon_start=1
                     /transl_table=11
                     /product="merD2"
                     /translation="MNAYTVSRLALDAGVSVHIVRDYLLRGLLRPVACTTGGYGLFDD
                     AALQRLCFVRAAFEAGIGLGALARLCRALDAANCDETAAQLAVLRQFVERRREALANL
                     EVQLAAMPTAPAQHAESLP"
     CDS             complement(5712..6350)
                     /note="Organomercurial lyase"
                     /codon_start=1
                     /transl_table=11
                     /product="merB2"
                     /translation="MKLAPYILERLTPTNRPKGFAEFLVALLGELAKGRPISRTTLAR
                     TLGWPAEQVAAVLEQATSTEYDNDGNIIGYVLTLRETSHVLEIDNRRLYAWCALDTLM
                     LPALIGRTARVSSHCAATGAPVSLTVSPSEIQAVEPAGMAVSLVLPQEAADVRQSFCC
                     HVHFFASVPTAEDWASKHQGLEGLAIVSVHEAFGLGQEFNRHLLQTMSSRTP"
     CDS             6419..6853
                     /note="Mercuric regulatory protein MerR"
                     /codon_start=1
                     /transl_table=11
                     /product="merR2"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLPEPDKPYGSIR
                     RYGAADVTRVRFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLQDVREKMAD
                     LARMEAVLSDLVCACHSRQGNVSCPLIASLQGGTSLAGASTA"
     repeat_region   6849..6886
                     /product="IRR_Tn21"
                     /note="Tn21 inverted repeat right"
     CDS             6938..7351
                     /note="Hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="orf414"
                     /translation="MVNRGLKDQIQDLQNRLIQAESRASELIDTNAQLQRQTHELEQA
                     NAMHKSTLDRQVADIQNLEHQLNDASAKNRERKHESKQLADNIAALASQKADADKQVA
                     VLSQALEVTKTDLKASQNRAEVLSDENKLILQKKR"
     misc_feature    complement(7617..7832)
                     /product="Mercuric resistance coregulator"
                     /note="merD1"
     CDS             complement(7829..8467)
                     /note="Organomercurial lyase"
                     /codon_start=1
                     /transl_table=11
                     /product="merB1"
                     /translation="MDKTIYSKKIAESLSSGNHPKEFATLFVALLRQLAMGGPVSREK
                     LAGALGWSGARVATVLEQAPGTEYDDEANIIGLGLTLRETSHVFEVDGRHLYTWCALD
                     TLMFPALTGKIARVTSPCAATGRPITLTVAPEAVLHVEPAEAMVSLRTPDTSPDIRCS
                     FCCHVHFFASPSIANSWASTHQGIEVVPVESAFDLGHDVALKLLEDCEESPV"
     CDS             complement(8681..9199)
                     /note="Mercury transporter periplasmic protein"
                     /codon_start=1
                     /transl_table=11
                     /product="merG"
                     /translation="MFCDIRLKLRRVRACLTIVLASTATLGMPNQALAAYDFSKLGAA
                     IETLSPETVSDWEKKARAGDAMAQNIMGLAYKCGMGVKQNHAASIQWFAGRPSRVKPM
                     HSSTSGASTEVKSTVCIRMGVRLLPTMQRLSSGTASLPSRDTRRLKSGWPSCLQRVLP
                     TSRPTRCRHTSG"
     CDS             complement(9235..10944)
                     /note="Mercuric reductase"
                     /codon_start=1
                     /transl_table=11
                     /product="merA"
                     /translation="MQELNEVGLSVAGMTCPSCTRHVEDALLAVPGVTRAAVDYPSNK
                     AQVTGNRLDVSALVAAVGALGYGATPTDEFESKERSVEQPGLLGKAAQWLSGERAVEK
                     PAGQLHVAIIGTGGAAVAAALKAAENGARVTLIERGTIGGTCVNVGCVPSKIMIRAAH
                     IAHLRRESPFDVGLSATPPVVLRDRLLAQQQARVDELRHAKYESILESNPSINLVRGS
                     ARFKDGQTLIVEAAEGDTREVAFDRCLIATGASAAIPPLPGLAGTPYWTSTEALVSEA
                     IPKRLAVIGASVVALELAQAFARLGSEVTVLARRTLFASDDPAIGEAVTAAFRAEGIT
                     VLTQTQASAVTYCDRQFILTTAQGELKVDQLLVATGRTPNTASLNLELAGVTFDSQHR
                     ILIDQGMRTSASNIYAAGDCTDQPQYVYVAAAAGTRAGINMTGGDVKLDLDAMPAVVF
                     TDPQVATVGYSEAEAQRIGLQTESRTLTLDNVPRALANFDTRGFIKLVAEAGSGRILV
                     VQAVTPEAGEIIQTAAIAIRARMTVQDLANQLFPYLTMVEGLKLVAQTFSKDVTQLSC
                     CAG"
     CDS             complement(11132..11407)
                     /note="Mercury transporter periplasmic protein MerP"
                     /codon_start=1
                     /transl_table=11
                     /product="merP"
                     /translation="MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCATCPITIKKAL
                     SKVDGVSKTEVDFSTKLAVVTFDDAKTNVQALSKATTDVGYPSELKK"
     CDS             complement(11421..11771)
                     /note="Mercuric transport protein MerT"
                     /codon_start=1
                     /transl_table=11
                     /product="merT"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     CDS             11843..12277
                     /note="MerR1, activator/repressor of mer operon MerR"
                     /codon_start=1
                     /transl_table=11
                     /product="merR1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLPEPDKPYGSIR
                     RYGEADVVRVKFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLQDVREKMAD
                     LARMEAVLSDLVCACHSRQGNVSCPLIASLQGGTSLAGASTA"
     repeat_region   12324..12342
                     /product="TniA-binding site 5"
     repeat_region   12347..12365
                     /product="TniA-binding site 6"
     repeat_region   12349..12373
                     /product="IRR_Tn5058a"
                     /note="Tn5058a inverted repeat right"
BASE COUNT     2570 a   3728 c   3664 g   2411 t
ORIGIN
        1 tgtcattttc agaagacgac tgcaccaatt gacggggcgt aacgccagat gtgcaggcga
       61 ctcctgacaa cgcaatatca gaagtcatct gcaccaatct cgactatgct caatactcgt
      121 gtgcaccaaa gcgaggtgtg agcatggcgt cagacacatc attgattgcc gagcaaggcg
      181 tggccaccct gcccgatgcg gcttgggcgc aggcccggca acgggcggaa atcatcgggc
      241 cgctggcagc gcttgatgtg gtcgggcatg aagccgccga tgccgctgct cacgcgcttg
      301 gcctgtccag gcggcaggtg tatgtcctaa tccgccgtgc ccggcaaggt gctgggcttg
      361 tgacggacct ggctcgcagc cgatccggcg gcggaaaagg caagggacgc ttgccggaat
      421 cagttgagcg catcatccgc gagttgctgc aaaagcgctt cctgaccaag cagaagcgta
      481 gcctggcagc gttccaccgc gaggtcgcgc aggcttgcaa agcgcaaaag ctgcgggcgc
      541 cggcgcgcaa caccgtggct ctgcggatcg ccggcctcga tccgctcaag gccactcgcc
      601 gccgggaagg tcaggatgcg tcccgcagcc tgcaaggtgt cggtggtgag cctcccgccg
      661 tgaccgcgcc actggaacaa gtgcagattg atcacacggt catcgacctg atcgtggtgg
      721 acgagcgcga ccggcaaccg attggccgtc cgtatctgac catcgccatc gacgtgttta
      781 cccgctgcgt gctcggcatg gtcgtcacgc tggaagcgcc gtcatctgtt tcggtcggcc
      841 tgtgccttgt gcatgtcgcc tgcgacaagc gtccctggct ggagggtctg aagatagaaa
      901 tggagtggcc gatgagcggc aagcccaggc tgctctacct ggacaacgcg gccgagttca
      961 agagcgaagc gctacgccga ggctgcgagc agcatggcat ccggcttgac tatcgcccgc
     1021 tcgggcagcc gcactacggc ggcatcgtgg aacggatcat cggcacggcg atgcagatga
     1081 tccacgacga attgccaggg acgaccttct ccaaccctga ccagcgcggc gactacgatt
     1141 ccgaaaacaa ggccgccctg acgctgcgtg agctggagcg ctggctcaca ttggcggtcg
     1201 gcacctacca cggctccgtg cacaacggcc tgctccagcc gccggcagcg cgctgggccg
     1261 aagctatcgc gcggaccggc gtgccaaccg tcatcactcg caccacggct tttctggtcg
     1321 attttctgcc catcatccgc cgcacgctga cccgcaccgg cttcgtcatc gaccacatcc
     1381 attactacgc cgatgcgctc aagccgtgga tagctcggcg cgaccgcttg cctgcgttcc
     1441 tgatccggcg cgacccgcgc gacatcagcc gcatttgggt gctggagccg gaggggcagc
     1501 actatctgga aattccatac cgcaccttgt cgcacccggc tgtcaccctc tgggaacaac
     1561 gacaggcgct ggcgaaattg cggcagcaag ggcgcgaaca ggtggatgag tcggcgctgt
     1621 ttcgcatgat cggccagatg cgcgaaatcg tgtccaccgc gcagaaagct acgcgcaagg
     1681 cgcggcgcga cgcggatcga cgccagcatc tcaaggcaac ggcagttctt ttcaaaacca
     1741 cgccaccacc ggacgcggac atggctgacc cgcaggcaga caaccagcca cctgccaaac
     1801 cgttcgacca gattgaggag tggtagccgt ggaagaatat cccatcatcg acttgtccca
     1861 cctgatgccg gtggcccagg gcttggcccg tcttccggcg gacgaacgca tccatcgcct
     1921 tcgcgctgac cgctggatcg gctatccgcg agcagtcgag gcgctgaatc ggctggaagc
     1981 cctgtatgcg tggccgaaca aacaacgcat gcccaacctg ctgttggtcg gtccaaccaa
     2041 caacggcaag tcgatgatcg tcgagaaatt ccgccgcacc cacccggcca gctccgacgc
     2101 cgaccaggag cacattccgg tactggtcgt gcagatgcca tccgaaccgt cggtaatccg
     2161 cttctacgtc gcgctacttg ccgcgatggg cgcgccattg cgcccgcgcc cacggctgcc
     2221 ggaaatggag caattggcgc tggcactgct acgcaaggtc ggcgtgcgca tgctggtgat
     2281 cgacgaattg cacaacgtcc tggccggcaa cagcgtcaac cgccgggaat tcctcaacct
     2341 gctgcgtttc ctcggcaacg agctgcgcat cccgctggtc ggggtcggca cacgcgatgc
     2401 ctacttggcg atccgctcgg acgaccagtt ggaaaaccgc ttcgagccga tgatgctgcc
     2461 ggtgtgggag gccaacgacg attgctgctc actgctggcc agcttcgcgg cttcgctccc
     2521 gctgcgccga ccctcgtcga ttgccacgct ggatatggcc cgctacctgc tcacgcgcag
     2581 cgagggcacc atcggcgagc tggcgcacct gttgatggcg gcggccgtcg ctgccgtgga
     2641 gagcggtgag gaagcgatca accatcgcac gctcagcatg gccgattaca ccggtcccag
     2701 cgagcggcgg cggcaattcg agcgggaact gatgtgaagc cagcgccaca ctggccactg
     2761 catccggctc ccagggaagg cgaagccttg tcttcgtggc tcaaccgcgt ggccctttgc
     2821 tatcacatgg aggtgtccga gctgctggag cacgatcttg gtcacggcca ggttgatgac
     2881 ctggacaccg cgccaccact ggcgctgctg gcgatgctct cccagcggag cggcatcgag
     2941 ccggaccggc tgcgttgcat gagtttcgcc ggctgggtgc cttggctact ggacagcctt
     3001 gatgatcaga ttccagatgc attggaaacc tatgcgttcc agctctcggt gctgctgccg
     3061 aaactccgcc gtaggacgcg atccatcacg agctggcgtg cctggctgcc cagccagccg
     3121 atacatcgcg cctgcccgct ctgtctgaac gatccggaga accaagccgt actgctcgcg
     3181 tggaagctgc ccctgatgct gagctgccca ctgcatggct gctggctgga atcctattgg
     3241 ggcgtgccag ggcggtttct cggctgggag aacgccgacg ccgaaccgcg cactgccagc
     3301 gacgcgattg cggcgatgga ccagcgtacc tggcaggcac tgacgaccgg ccacgtggag
     3361 ttgccgcgcc gacgcatcca cgccgcattg tggtttaggc tgctacgcac gctgctcgat
     3421 gagctgaaca ccccgctttc gacgtgcggc acctacgcgg ggtatctccg ccaaatctgg
     3481 gaatgctgcg ggcatccgct gcgtgctggg caaagtctgt ggcgaccgta tgaaaccctg
     3541 aacccggcag tacggttgca gatgctggag gcggcggcaa cggcaatcag cttgattgag
     3601 gtgagggata taagcccgcc aggcgagcac gcaaagctat tctggtccga gccccaaacc
     3661 gggttcacca gtggcctgcc ggcgaaagcg ccgaagcccg aacccgtcga tcactggcag
     3721 cgtgcaatcc aggccattga tgaggccatc attgaagcac gacacaaccc cgagacggca
     3781 cgctcgctgt tcgcgttggc ttcctatggt cggcgcgacc ccgcttcctt ggaacagttg
     3841 cgcgccacct tcgcgaagga aggcatcccc ccggaatttc tgtcacatta tgagcctagc
     3901 ctaccctttg catgccttag acagaatgac gggttaagtg acaaattttg acgtgcagag
     3961 cttcccgatg caaactgtca cataatcgaa cgtatatgtg acaggtacaa gatgctgatt
     4021 ggctacatgc gagtatcgaa ggcggatggc tcccaggcga cggacttgca gcgcgatgcg
     4081 ctggtcgcgg ccggtgttga tccggcgcat ctctatgaag accaagcgtc cggaaaacgc
     4141 gaggatcgtc ccggtctggc gagctgtctg aaggcactac ggccaggcga cacgttggtc
     4201 gtttggaaac tggatcggct cgggcgcgac ctgcgcaatc tgatcaatac cgtccatgac
     4261 ctgaccgaac gcggcatcgg cctcaaggtg ctgaccgggc acgtcgcggc cattgacacc
     4321 acgaccgccg ccggcaagct ggtcttcggt atcttcgccg cgctggccga gttcgagcgc
     4381 gagttgatcg ccgagcgcac cgtggcgggc ctggcctcag cacaaacacg cggccggaaa
     4441 ggcggccggc cgttcaagat gaccgccgcc aagctgcggc tggcgatggc ggcaatgggg
     4501 cagtcacaga ccaaggtcgg cgacctgtgc caggaacttg gcatcacgcg gcagaccctg
     4561 tatcggcata tttcacccaa gggcgagcta cgtccagatg gcgagaagct actcagccga
     4621 atttgatgcc gacatgaggc gacgtagcga aagcgtggtt tgtctcaatt tgacggcggc
     4681 gaaccgcaag cgttcggttc gccgctcgtt tcggtcgcag tgctggcccg cgcctggaaa
     4741 cgttgataac actccagccc gcagaagtgc tcgacgtact ccgcgccttc cggtgtgaag
     4801 gcggcatcga gcggaatttc ctttgaccag cacacgcagc aactggtggc ggtcggatca
     4861 tttgcattca tggtggcacc cctccattga ctgacgaaga cggcgaatgc cgcgccggca
     4921 tcggctttgc gaacagaaag ccttgcccgc gctcgaacgc ttgggccctc cgccgtagtc
     4981 catccatccg gccgggaagc gctcatgatc tttccctgaa tgcccgcaac gcccgcgcca
     5041 gtgacagaag aaacaggccg gtcaaaccga gcgccgtgat gacccaatac tcgacaagga
     5101 atgcaccggc ggttgtgccg gccagcacgg cagcgaggat ggacaggtgg caggggcaag
     5161 tcagcacggc cagcgtgccc cacaggtagc cggtgaacgg tttgtgcgtc tcggccggga
     5221 tgcgctcggg gctgttcatg gcaaactctc cgcatgctgt gccggcgcgg tcggcatggc
     5281 ggccaactgc acttccagat tggccaacgc ttcgcgccgg cgttcgacga actgacgcag
     5341 cacagcaagc tgcgcggcag tttcatcgca gttcgccgca tccagcgccc ggcacagccg
     5401 cgccaatgcg ccgaggccga tgcccgcctc gaaggcggcc cgcacgaagc acagtcgctg
     5461 caaggcggcg tcatcgaaca ggccgtagcc acccgtggtg caggcgactg gccgcagcaa
     5521 tccgcgcagc aggtagtcgc gcacgatatg cacgctcacc ccggcatcaa gggccagccg
     5581 ggacaccgtg taggcgttca tcgaacacct ccttttggtc ggttcacggc aatgcatata
     5641 ccgtttcgcc gagttcaatc cggcgctgcg aatccgatgc cggtgccgta gaacattggg
     5701 tcgatatccg atcacggtgt cctagatgac atggtctgca acagatgtcg attaaactcc
     5761 tggcccaagc cgaaagcctc gtggacactg acgatcgcca atccttccaa tccttgatgc
     5821 ttggaggccc agtcttccgc cgtcgggaca gatgcaaaga aatgtacatg gcaacagaag
     5881 gactgacgaa cgtcggctgc ttcctgcggc aataccaagg acaccgccat gccggcaggt
     5941 tcgacagcct gtatctcgct gggtgaaacc gtaagcgaaa cgggtgctcc agttgcagcg
     6001 caatgcgacg agacgcgagc tgtacggccg attaacgccg ggagcatcag ggtgtccagc
     6061 gcgcaccagg catacagccg gcggttgtca atttccaaga catgcgacgt ctcgcgcaag
     6121 gtgaggacat agccgatgat gttcccatcg ttgtcgtatt cggtactggt ggcttgttcg
     6181 agcacagccg ccacttgctc agcgggccag ccaagagtcc tggcaagcgt agttcgcgaa
     6241 atcgggcgtc ccttggcaag ttcacccagt agcgcgacca agaactccgc aaaacctttg
     6301 ggccggttgg tcggggtgag acgttctaaa atatatgggg cgagcttcat ggttccatct
     6361 cctatttgaa ttggatagcg taaccttacc ttcgtactca tgtacggagt caagcggtat
     6421 ggaaaataat ttggagaacc tgaccattgg cgtttttgcc aaggcagccg gcgtcaacgt
     6481 ggagaccatc cggttctatc agcgcaaggg cttgttgccc gagccggaca agccctatgg
     6541 cagcattcgc cgctatggcg cggcggacgt gacacgggtg cgattcgtga aatcggccca
     6601 gcggttgggc ttcagcctgg atgaaatcgc cgagttgctg cggctcgacg atggcaccca
     6661 ctgcgaggag gccagcagcc tggccgagca caagctccaa gacgtgcgcg agaagatggc
     6721 cgatttggcg cgcatggagg ctgtgctgtc tgacctggtg tgcgcctgcc attcgcggca
     6781 ggggaatgtt tcgtgtccgc tgattgcgtc gttgcaaggt ggaacgagct tggcaggggc
     6841 ttccacagct tagcgtgctt tattttccgt tttctgaggt gccccctacc agcaacgcac
     6901 cgccgaagac cggcagcagg agcgcgagca attccagatg gttaaccggg gcctgaaaga
     6961 ccagattcaa gacctgcaaa accggctcat ccaagccgag tcaagagcgt ccgagctgat
     7021 agacactaat gcgcaattac aacgacaaac gcatgagctc gaacaggcca atgcgatgca
     7081 caaaagcacc ttggataggc aagtggccga tatccagaac ttggagcatc aacttaacga
     7141 cgcctcggca aaaaaccggg agcggaagca tgaaagcaaa caattggcgg ataatattgc
     7201 cgcactcgca agccagaaag cagatgccga taagcaggtg gccgtactat cccaagcatt
     7261 agaggtcacc aaaacggatc tgaaagcctc tcagaataga gcggaagttc tgtccgatga
     7321 gaacaagttg attcttcaga aaaagcggtg attcaggggc agtttaagca gcttcaggag
     7381 tcactgtcgc gataaaggct ttagtcaaac attcatcgaa aaagctatat taatcaatat
     7441 attatatacg aagtggctaa ttccaacgct tttaccggcc ggacctcaag tagctgcacc
     7501 ggccgccatt tcattcaaag aggcctcgac gacagtcaaa actgttcgac gcacgtcgat
     7561 cttccatcgc acgcgctcga cgcatcggtc caggctttcg ctatcgtctg cgtccaatgc
     7621 tttgcaaagt cgcgccagtt cgtcgagccc aattccggac tcgaaggctg cgcggagaaa
     7681 ccgcagcctt ccgagtgttt tgtcatcgaa aatgttctag ccactttcgg ttcgccgagc
     7741 agggtgcaac aagccgcgta tcatgtagtc gcgcacgaca tgcactggac gcaagcatca
     7801 tcagccagct tcgatatcga gtacgcattc atactgggct ttcctcgcag tcctctagca
     7861 gcttaagcgc gacatcatga ccaaggtcga acgcactctc gacaggcacg acctcaatcc
     7921 cttggtgggt cgaagcccac gaatttgcaa tagacggaga cgcaaagaaa tgcacatgac
     7981 agcaaaacga acaacgaatg tcgggcgaag tatccggagt tcgcagtgaa accattgctt
     8041 ctgccggttc gacatgaagc actgcttctg gtgcaacggt gagagtgatc ggcctaccgg
     8101 tggcggcgca cggggaagtg acacgggcaa tcttgccggt caaggcggga aacatcaagg
     8161 tgtccagcgc acaccatgtg tacaaatgac ggccatccac ctcaaaaacg tgagaggttt
     8221 cacgtagtgt cagccccagt ccaatgatat tggcctcgtc gtcatattcc gtaccaggtg
     8281 cctgctcaag taccgttgca actctcgctc cagaccatcc gagtgcgccg gcgagctttt
     8341 cgcgtgatac aggtcccccc atcgctagct gtcggagcag cgcgacaaag agcgttgcga
     8401 actctttagg atgattgcca gatgaaaggc tttcggcaat ctttttggaa taaatagtct
     8461 tgtccatttt ccacctgtta tatcgggcag cgatcaaccg ggataaacct tactcggcgg
     8521 ttacgtcctg cgcttaacaa agacgaatta ctttgtcctt gccggcgttg ccattcctcg
     8581 gccagcagct gggcttgctg taactgttcg ggcttcattt gagctgcata gtctgcaacg
     8641 acttttgctg ctgtcggttc tcccgacgct gcggccagac tcatccactg gtatgcctgc
     8701 acctggtcgg gcgcgatgtc ggtaagaccc tttgcaaaca gctgggccag cctgacttga
     8761 gcctgcgtgt gtccctgctc ggcagcgagg cggtaccact tgaaagcctc tgcatcgttg
     8821 gcaggagccg cacgcccatt cttatacata ccgtcgactt cacttccgta gaggcgcccg
     8881 aggttgaact gtgcatcggc ttcaccctgc tcggccgccc tgcgaaccac tggatcgatg
     8941 ccgcgtggtt ttgctttacg cccataccgc atttgtacgc aagacccatg atgttctgag
     9001 ccatggcatc gccagccctg gccttctttt cccaatcact gacggtctct ggcgacaatg
     9061 tttcaatcgc ggcgccgagc ttagaaaaat cataagccgc caacgcttgg tttggcatgc
     9121 ctaacgttgc ggtacttgcc agtacgatcg tcaagcacgc gcgtacacgg cgtaatttaa
     9181 gtctgatgtc acaaaacata aaaactcctc tgtccctgtt ttggtgccca aggtttagcc
     9241 ggcacagcac gatagctgtg tcacatcctt cgaaaaagtc tgtgccacca gtttgagacc
     9301 ttcaaccatg gtgaggtaag ggaaaagctg gttagccaga tcctggacgg tcatgcgggc
     9361 ccggatggca atcgcggcag tctggatgat ttcgcctgct tccggtgtca cagcctggac
     9421 tacgagaatg cgtccggacc ctgcctcagc aacaagcttg ataaagccgc gggtatcgaa
     9481 gttggcgagt gcacgcggca cattgtcgag cgtgagcgta cggctttcgg tttgcagacc
     9541 aatgcgctgg gcctctgctt cactgtagcc taccgtggca acttgcggat cggtgaacac
     9601 gactgccggc atggcgtcga gatcgagctt tacgtcgccg ccggtcatat taatgccagc
     9661 ccgggtacca gccgcggctg caacgtagac gtactgaggc tggtcagtgc agtcgccagc
     9721 tgcgtaaatg ttcgaagcac tcgtgcgcat gccttgatcg atcaggatgc gatgttgcga
     9781 gtcaaacgtc acacctgcga gctcgagatt caggcttgcg gtatttggcg tgcggccggt
     9841 ggcaacaagc aattgatcca cctttaactc gccctgcgcg gtcgtcagga tgaattggcg
     9901 gtcgcaatag gtcacggcac tcgcctgcgt ctgtgtcagc accgtgatgc cttccgcacg
     9961 gaaagctgca gtcacggcct cgccaatggc agggtcgtca ctggcaaaca aggtgcgccg
    10021 ggccaggacc gtcacctcgc tgcccaagcg cgcaaacgct tgtgccagtt ccagtgctac
    10081 gaccgaggcg ccaattactg ccagccgttt aggaatggct tcgctaacca gggcttcggt
    10141 ggacgtccaa tatggcgttc ctgcaagacc aggcaacggc gggatggcag cgcttgcacc
    10201 agtggcaatg agacagcgat cgaaggccac ttcgcgagtg tcaccttctg ccgcttccac
    10261 gataagcgtt tggccatcct tgaatcgggc gctgcctcgc accagattga tcgacggatt
    10321 gctttcgagg atgctttcat atttagcatg acgcagctcg tccacacggg cctgctgttg
    10381 cgccagcaga cggtcacgca ggacaactgg gggcgttgcc gacagtccca cgtcaaaagg
    10441 actttcgcgt cgcagatgag cgatatgcgc tgcccgaatc atgatcttag aagggacgca
    10501 gcccacgttg acgcaggtgc cgccgatagt tccccgctcg atcagggtga cacgggcacc
    10561 gttttcagct gccttcagcg ctgctgccac agcggcgccg ccagtaccga tgatcgccac
    10621 atgcagctga cctgccggtt tctccaccgc gcgctcgccg ctcagccact gggcggcttt
    10681 gccaaggagt cctggctgtt caacactgcg ctctttgctt tcaaattcgt ctgtaggcgt
    10741 agcaccgtaa ccaagggcgc ccactgctgc tacgagcgcg gacacgtcaa gcctattgcc
    10801 tgtgacttgc gccttgttcg atggataatc gacagccgct cgcgttacgc cggggaccgc
    10861 cagcagcgcg tcctcaacgt gccttgtaca acttgggcaa gtcattcccg ccacgctcaa
    10921 accaacttcg tttaactctt gcattccgct ctctctttta tccgttgtgc ccaagccaac
    10981 tagtacgacg cctcggtcac tgaatcggtt gtgcgcttca gggaacaggc tccgtgggtc
    11041 gtttacaggc tgctcacgcg atgacgaccg gcagaatcgc ctgagcggca tctctgccgt
    11101 ggttctagcg atgctgccgt tatttgttgc cttacttctt cagctcagat gggtagccta
    11161 catccgtcgt cgccttgcta agcgcctgga cgttggtctt cgcatcgtca aacgttacga
    11221 cggcaagctt ggttgagaag tcgacctcgg tcttgctaac accgtcgacc ttgctcaagg
    11281 cctttttaat ggtgatagga caagtcgcgc aggtcatgcc aggaacggcc aaggtgaccg
    11341 tctgcgtggc ggcccacacc ggggcaacag cggcggcgag ggcaagggag gcaaacagtt
    11401 tcttcatgat gaactcctgg ttaatagaaa aatggaacga catagggaaa tccaagcgcg
    11461 accaggacca gcacggccac gatccagaaa atcagcttgt aggtggcgcg cacctgcgga
    11521 atcgcgcaga cctcacctgg cttgcatgcc tgcacgggcc ggtaaatccg cttccaggcg
    11581 aagaacagcg ccactagcgc cgcgccgatg aacaacggtc gatagggttc cagcaccgtc
    11641 aggttgccga tccaagcacc ggagaagccc agggcgacca gtactagcgg ccccaggcag
    11701 caggtcgatg caagaatggc ggccagcccg ccggcgaaga gcgcaccgcg cccgttttgt
    11761 ggttcagaca tacgttggcc cttttgaatt tggattggat agcgtaacct tacttccgta
    11821 ctcatgtacg gagtcaagcg atatggaaaa taatttggaa aacctgacca ttggcgtttt
    11881 tgccaaggcg gccggggtca acgtggagac aatccgcttc tatcagcgca agggcctgtt
    11941 gccggaaccg gacaagcctt acggcagcat ccgccgctat ggggaggcgg acgtggttcg
    12001 ggtgaaattc gtgaaatcgg cacagcggct ggggttcagt ctggacgaga ttgccgagct
    12061 gttgcggctc gacgatggca cccactgcga ggaggccagc agcctggccg agcacaagct
    12121 ccaagacgtg cgcgagaaga tggccgattt ggcgcgcatg gaggctgtgc tgtctgacct
    12181 ggtgtgcgcc tgccattcgc ggcaggggaa tgtttcgtgt ccgctgattg cgtcgttgca
    12241 aggtggaacg agcttggcag gggcttccac agcttagcgt gctttatttt ccgttttctg
    12301 agacgaccac agatcagaaa agactgtgca gccgactctt gatattccgt gcagtcacct
    12361 tctgaaaatg aca
//
