LOCUS       Tn4662a                 7196 bp    DNA     linear       29-SEP-2020
DEFINITION  Pseudomonas sp. LM13.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Pseudomonas sp. LM13
  ORGANISM  Pseudomonas sp. LM13
            Unclassified.
REFERENCE   1  (bases 1 to 7196)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn4662a
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7196)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..7196
                     /organism="Pseudomonas sp. LM13"
                     /mol_type="genomic DNA"
     repeat_region   1..38
                     /gene="IRL_Tn4662a"
     CDS             complement(33..3062)
                     /gene="tnpA"
                     /codon_start=1
                     /product="Tn4662a transposase"
                     /translation="MSNKNKLLTVFSDAEQEALYGLPDFDDAQRLEYLALTESELAFA
                     SSRPSLQAQVYCVLQIGYFKAKHAFFRFDWHEVEDDCAFVLSRYFHGEAFERKAITKH
                     EHYSQRGQIAELFGYRSWAASFLPQLAQQAEQIVRRDVMPGFVAAELIVWLSEHKIIR
                     PGHTTLQELVSEALSTERRRLGGLLAEVLDESAKAALGQLLVRDDTLSQLAALKQDAK
                     DFGWRQMAGEREKRATLKSLHGIAKALLPKLGISQQNLLYYASLANFYTVHDLRHLKA
                     EQTRLYLLCYAWVRYRQLTDNLVDAMAFHMKKLEDESRTGAKQSFVAEQLRRHQETPQ
                     VGRLLSLYVDDSVADPTPFGEVRQRAYKIMSRELLQNTAQRMSVKPLNKLALHWQAVD
                     GLAERIRRHLRPLYVALDFAGTAPDNPWLAALTWAKSVFAKQQRLSQRPLDECPAATL
                     PKRLRPYLLMFDAEGTPTGLHADRYEFWLYRQVRKRFQAGELYIDDSLQHRHLSDELV
                     SMDEKAAVLAQMDIPFLRQPVSAQLDALAAELRAQWVAFNRELKQGKLTHLEYDKDTQ
                     RLTWRKPKGENQKAREQALYEQLPYCDVADVFRFVNGQCQFLSALTPLQPRYAKKVAD
                     ADSLMAVIIAQAMNHGNQVMARTSDIPYHVLESTYQQYLRQATLLVANDCISNAIAAL
                     PIFPHYSFDLDSLYGAVDGQKFGVERPTVKARYSRKYFGRGKGVVAYTLLCNHVPLNG
                     YLIGAHEYEAHHVFDIWYRNTSDIVPSAITGDMHSINKANFAILHWFGLRFEPRFTDL
                     DDQLQELYCADDLALYEKCLIRPAGQIDRQLIVGEKANIDRIVATLGLKEMTQGTLIR
                     KLCTYTAPNPTRRAIFEFDKLIRSIYTLRYLRDPQLERNVHRSQNRIESYHQLRSTIA
                     QVGGKKELTGRTDIEIEISNQCARLIGNAIIFYNSAILSLLLTKYEAAGNAKALALIT
                     QMSPAAWRHILLNGHYTFQTDGKFIDLDALVAGLELG"
     CDS             complement(3046..3681)
                     /gene="tnpR"
                     /codon_start=1
                     /product="Tn4662a resolvase"
                     /translation="MRKPVFDGYPPMLVGYMRVSSDSDRQSTDLQRDALLAAGVDPRH
                     LFEDRASGAKDDRAGLARALEFVRAGDVLVVWKLDRLGRSLSHLLAIVTSLKDKRVAF
                     RSLTENLDTTTPSGEFLFQVFGALAQYERALIQERVVAGLAAARKRGRIGGRPQAITG
                     EKLDAIVAALDGGMSKAAVCRNFNVKRTTLIETLTRAGWRGAGRTVDEQQE"
     misc_recomb     3673..3784
                     /gene="res"
     CDS             3840..4217
                     /gene="relE"
                     /codon_start=1
                     /product="Toxin RelE"
                     /translation="MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDA
                     AKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAVFVLHCFQKKSKSGIATPKADMD
                     IIRARLKVAEVLAQELRNAKTNH"
     CDS             4198..4533
                     /gene="relB"
                     /codon_start=1
                     /product="Antitoxin RelB"
                     /translation="MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAM
                     RALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERKLMDCLNRLGYDIEIKVRPAAEP
                     IGHLTLATA"
     CDS             4548..4883
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MAARITDDEWDELTPENFDTTALLRAVDAVDVLRGDLNDSADGA
                     PPQLRTDLLKLHQLAMAAFNEGSRSRVAELFDLAVDLQDQVDHLMTSLEQVQETLSRL
                     TALYPESLS"
     CDS             4907..5233
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MSRSRRKTPIVGHTTCRSEREDKKLWHQRWRTHERTALASASPK
                     ALCAHLPLLENQVSNVWSMGKDGRSYWPIKRQAATADRIANHKGRNPQERASLKKRLL
                     RKWMSK"
     CDS             5230..5610
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MNLSTIEALAIAWARIAEEAELPAGYEGTATPEAHRACEVIQER
                     IREHVVATNDMRLFGLLHLLGQASLRMEQALWPEEYARMTREVEEALREADDPNAKSY
                     THEEVMQAMQERIDRARDKAMLIG"
     CDS             5767..7029
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MYIHKLAGSWVRHPFWRGSFLLTEPQDLSAIRECGVGEVWVDLA
                     KSQVDPESPESPESPESPESPESPESPESPESPESPEPRAQSPEPRELSEEQSLPSSP
                     LSKKSDGATSMESEMCYARKLCLAAKSQVMDMFQEARLGKAVDPSTTLPLVGEIAASV
                     LRQPHALISVARIKTHDDYTYLHSVAVCALMLSLARHLDLDEEQTRLAGIGGLMHDLG
                     KAAMPLEVLNKPGKLTDAEFAIMKCHPVEGAKMLRAGGAEPGVVDIALHHHEKIDGTG
                     YPDRLAGDAISLLARMGAICDVYDAVTSERAYKKPWDPSAAMRQMAKWEGHFDKRIFH
                     AFVKAVGIYPVGSLVRLSSQRLAVVVEPGMESLLTPKVRVFFSLRSREPIPMQTIDLA
                     ATSCKDSITGPEDPTLWNFKNLDDLWME"
     repeat_region   7159..7196
                     /gene="IRR_Tn4662a"
ORIGIN      
        1 ggggttgtac gccggaatcc cagaaatttc cgtcagccca gctccagtcc cgccacgagc
       61 gcatccaggt cgatgaactt gccgtcagtc tggaatgtgt aatgcccgtt cagcaggatg
      121 tgccgccagg ccgctggcga catctgcgtg atcaacgcca gcgccttggc attgccagct
      181 gcctcgtact tcgtcagcag cagggacagg atcgccgagt tgtagaagat gatcgcgttg
      241 ccgatcagcc tggcgcactg gttgctgatc tcgatttcga tgtcggtgcg gccggtcaat
      301 tccttcttcc caccgacttg ggcgatggtc gagcgtagct ggtgatagga ctcaatgcgg
      361 ttctgcgagc ggtgaacatt acgctccagt tgcgggtcgc gcaggtagcg cagtgtgtag
      421 atactgcgga tgagcttgtc gaactcgaag attgcgcgcc gcgtcgggtt cggcgccgta
      481 taggtgcaca gcttgcggat cagcgtgccc tgcgtcattt ccttcaggcc cagtgtggcg
      541 acgattcggt cgatgttcgc cttctcaccg acgatgagtt gccggtcgat ctggccagcc
      601 ggccggatca ggcatttctc gtacaatgcc agatcatcgg cgcaatacag ctcctgcaac
      661 tggtcgtcga ggtcggtgaa gcgcggctcg aaacgaagtc cgaaccagtg caggatggcg
      721 aagttggcct tgttgatgct gtgcatgtcg ccggtgatcg cgctcggcac gatgtccgac
      781 gtgttgcggt accagatgtc gaacacgtgg tgagcctcgt actcgtgtgc gcctatcagg
      841 tagccgttca acggcacgtg attgcacagc agcgtgtagg cgacgacgcc cttgccgcgg
      901 ccgaaatatt tgcgcgagta gcgcgccttc acagttggtc gctcgacgcc gaatttctgc
      961 ccatcaacgg caccgtacaa cgaatcgagg tcgaacgagt aatgcgggaa gatcggcagt
     1021 gcggcgatgg cgttgctgat gcaatcgttg gccacaagta gcgtcgcctg gcgcaggtac
     1081 tgctggtagg tactctccag gacgtggtac gggatgtcgc tggtacgtgc cataacctgg
     1141 ttgccgtggt tcatggcttg ggcaatgatc accgccatca gactgtcggc gtcggctacc
     1201 ttcttcgcat agcgtggctg caatggtgtc agcgccgaca ggaactggca ctggccgttg
     1261 acgaagcgaa acacgtcggc gacatcgcag tatggcagtt gctcgtagag agcttgctcg
     1321 cgcgccttct ggttctcccc cttgggcttg cgccaggtca gtctctgcgt gtccttgtcg
     1381 tattccaggt gcgtcagctt gccctgtttc agctcgcgat tgaacgccac ccattgcgca
     1441 cgcaactcgg ccgccagtgc atcgagctgg gcactgaccg gctgccgcag gaaggggatg
     1501 tccatctgcg cgagcacggc ggctttctcg tccatcgaaa ccaactcgtc ggacaaatgc
     1561 cggtgctgca agctgtcgtc aatgtagagc tcgcccgcct ggaagcgttt cctgacctga
     1621 cggtaaagcc agaattcgta acgatcggca tgcaggcctg tcggcgtgcc ttcggcatcg
     1681 aacatcagca ggtacggacg caagcgtttc ggcagcgttg ccgccggaca ttcgtcgagt
     1741 ggccgttgtg ataggcgctg ctgcttggcg aacacgctct tggcccaagt cagcgccgcg
     1801 agccatgggt tatcgggggc cgtgccggcg aagtcgagcg cgacgtacag cggccgtaga
     1861 tggcgtcgaa tgcgttcggc caggccgtcc accgcctgcc agtgcagcgc cagtttgttc
     1921 agtggcttga cgctcatgcg ctgcgccgtg ttttgcagca attccctgga catgattttg
     1981 taggcgcgtt ggcgcacctc gccgaacggc gtcggatcgg ccacgctgtc gtccacgtac
     2041 agcgacagca ggcggccaac ctgtggcgtt tcctgatggc gtcgcagctg ttcggcgaca
     2101 aaggactgtt tcgcacccgt gcggctctcg tcctcaagtt ttttcatgtg gaaggccatc
     2161 gcgtcgacca ggttgtcggt gagctgccgg taacgtaccc aggcatagca cagcaggtag
     2221 agccgggtct gctccgcctt caggtggcgc aggtcatgga cggtatagaa gttcgccaag
     2281 ctcgcgtagt acagcaggtt ctgctgcgag atgccgagct tgggcagcag cgccttggcg
     2341 atcccgtgca aggacttcag cgtggcgcgc ttctcgcgct cccctgccat ctgacgccag
     2401 ccgaaatctt tagcgtcctg cttgagcgct gccagttgag acagggtgtc gtcacgcacc
     2461 aggagctggc ccagcgcagc tttggccgat tcgtccaaca cttctgccag caagccgccc
     2521 aagcgcctgc gttcggtgga cagggcttca ctgaccagct cttgtaaggt ggtgtggccg
     2581 ggccggatga ttttgtgctc gctgagccaa acgatcagtt cggcggccac gaatcctggc
     2641 attacgtcgc gccgcacgat ctgttcagcc tgctgtgcca gttgcggcag gaagctagcc
     2701 gcccacgacc ggtagccgaa cagttcggcg atctgacccc tctggctgta gtgctcatgc
     2761 ttggtgatcg ccttgcgttc gaacgcttcg ccgtggaaat agcgactcag cacgaaggcg
     2821 caatcgtcct cgacctcatg ccaatcgaag cggaagaaag catgcttggc cttgaagtag
     2881 ccgatctgca agacgcaata gacttgggcc tgcaggctag gccggctgct ggcgaacgcc
     2941 agttcagatt cggtcaacgc caggtattcc agccgctgag catcgtcgaa gtccggtagg
     3001 ccgtacaagg cttcctgctc tgcgtcagaa aagacggtga gtagcttatt cttgttgctc
     3061 atcgaccgtc cttcccgcac cacgccaacc tgctcgcgtc aatgtttcga tcaacgtagt
     3121 gcgcttgaca ttgaagttgc ggcacaccgc cgccttagac atgccgccat cgagcgcggc
     3181 gacgatggcg tccagcttct caccagtgat cgcctgcggc cggccgccga tccggccgcg
     3241 tttgcgtgcg gcggccaagc ccgcgacgac gcgctcctga atcaaggcgc gctcgtactg
     3301 cgcgagcgca ccgaacacct ggaacaggaa ttcgcccgag ggcgtcgtag tgtccaggtt
     3361 ctctgtcagc gagcggaacg ccacccgctt gtccttgagc gaagtcacaa tggcgagcag
     3421 gtgcgacagc gagcgaccga gccggtcgag tttccacacc accagcacat cgccggctcg
     3481 aacgaactca agcgcccgcg ccaagccggc acggtcatcc ttcgcgccag aggcacgatc
     3541 ctcaaacagg tgacgcggat cgacgccggc ggcgagcagc gcgtcgcgct gcaagtccgt
     3601 gctctggcgg tcggagtccg acgacacgcg catgtagcca accaacatag gcggataacc
     3661 atcaaaaaca ggtttccgca taatagtgaa tagcgataga gttttccgta catttttgag
     3721 ggggtgttgc acaggtagca cagcggcggc tgacagcctg tcgcaaaaca aatgttttac
     3781 gacacctcct tgaggttgcc gcgtttacat cattacctga acaggtataa aattccgtca
     3841 tgacaaacaa agaaaaaccg ctcgaatgga tcgcgagcag ccacaaggat ttgatggcgt
     3901 tgccgtccga cgtgcgtcgc cgtttcggtt acgcgctctc gttggcgcag ataggcgatc
     3961 aggatgacgc agcaaaggtg ctcaaggggt tcggtggtgc cggcgtgctg gaggtcgtcg
     4021 aagacgatgc cggcggcacc tatcgagcgg tatacacggt caagtttgcg gaagcggtgt
     4081 tcgtcctgca ctgcttccag aagaagagca agagcggaat cgccacgcca aaggccgaca
     4141 tggacatcat ccgcgctcgg ctgaaggtgg ccgaggtatt ggcacaggag ctacgaaatg
     4201 caaaaacgaa tcattgaagg cgtcgaggtt cagcgcagct cgggcaacgt ctttgccgac
     4261 cttggactgc ctgacgctga aaagctcaag atcaagaccg gcctggtggt cgagatcagg
     4321 agggccatgc gcgcccttgg gctgactcaa caagcggcgg ccaaacgcat gggcatcccg
     4381 caaccgaagg tgtcgggcat gatgcgcggc gacttcacca atctatccga acgcaagctg
     4441 atggattgtc tgaatcgcct cggctacgac atcgaaatca aggtacggcc agcagccgag
     4501 ccgatcgggc atctaacgct cgcaaccgct taatcggagc cctcctgatg gcagcccgca
     4561 tcaccgacga cgaatgggac gaactgactc ccgagaattt cgataccacg gcactgctgc
     4621 gtgcagtcga tgccgtggac gtgctgcgcg gcgatttgaa tgacagcgca gacggcgcac
     4681 ctccgcaact gcgcaccgac ctgttgaagc tgcatcaact ggcaatggcc gcgttcaacg
     4741 agggatcacg cagccgagtg gctgagctat ttgatctcgc cgtggatctt caggatcagg
     4801 ttgatcatct gatgacctcg ctggaacaag tgcaagaaac cctgtcccgg ttgacggcgc
     4861 tctacccaga aagcctgtcc tgaatgttct tggagacaac cacatcatga gccgcagccg
     4921 ccgcaaaacg cccatcgttg ggcacacgac ctgccgtagc gagcgcgagg acaagaaact
     4981 ctggcatcag cgctggcgaa cccacgagcg caccgcgctg gccagcgcgt cgccgaaagc
     5041 tctatgcgcc catctgcctc tactggaaaa ccaggtcagc aacgtctggt cgatgggtaa
     5101 ggatggccgc tcctactggc ccatcaagcg ccaggccgcc acggcggatc gcattgccaa
     5161 ccacaaggga cgcaatccgc aagagcgcgc ctccctgaaa aagcgtctgc tgcgcaagtg
     5221 gatgagcaaa tgaacctgtc caccatcgag gcgctggcta tcgcttgggc gcgaatcgct
     5281 gaagaagcag aactcccagc cggctacgag ggcacggcga cgccagaggc gcatcgggcc
     5341 tgcgaggtga tccaggagcg gattcgagag cacgtcgtcg ccaccaatga catgcggctg
     5401 ttcggcctgc tgcacctgct tgggcaggcg tcgctgcgca tggagcaagc gctgtggccg
     5461 gaagaatatg cgcggatgac ccgcgaggtc gaggaagctc tccgagaggc cgacgacccc
     5521 aacgccaagt cgtacaccca cgaagaagtc atgcaggcga tgcaggaacg catcgaccga
     5581 gcgcgagaca aagccatgtt gatcggctga cggaaatttc ggcggttccg gctgatccaa
     5641 gaatcttggc gtgaccttac ctccgctcta ccgctgggtg ccagcctcgg cgcacgccta
     5701 accacccaac tcagaaagga aaataagttg gtaaaacgca ttcccgtatc cgagcttcgt
     5761 ctcggcatgt acatccacaa gctcgccggc tcttgggttc gacacccatt ctggcgcggc
     5821 agcttcctgc tgaccgagcc tcaggatctc tctgccattc gagaatgtgg cgtcggggag
     5881 gtctgggtcg acttggccaa aagccaagtc gacccagaga gcccagagag cccagagagc
     5941 ccagagagcc cagagagccc agagagccca gagagcccag agagcccaga gagcccagag
     6001 agcccagagc ccagagccca gagcccagag cccagagaat tgtcggagga gcaatctctg
     6061 ccgtctagtc cactaagcaa gaaaagtgac ggcgcaacct caatggagag cgaaatgtgt
     6121 tatgcacgga aactctgtct tgcggccaag tcccaagtca tggacatgtt ccaggaagcc
     6181 cggcttggca aggccgtcga cccaagcacg acgttgccgc tggtcggaga aatcgctgcc
     6241 tcggtgctgc gccaacctca tgccctcatc agcgtcgcac gcatcaaaac gcacgacgat
     6301 tacacctacc tgcactcggt tgccgtctgc gccctgatgc tatcgctggc ccggcatctc
     6361 gatctagacg aggagcaaac gcgcctggct ggcatcggcg gactgatgca cgacctaggc
     6421 aaggccgcga tgccgctgga agtgcttaac aaaccaggca agctcaccga tgccgagttc
     6481 gccatcatga agtgccaccc tgtggagggc gcaaagatgc tgcgcgcagg cggggccgag
     6541 cccggggtgg tggacattgc cctgcaccat cacgagaaga tcgacggaac cggctacccg
     6601 gatcgcttgg ccggtgacgc catttcactc ctggcccgca tgggcgcaat ctgcgacgtc
     6661 tatgatgccg tgacgtcgga gcgagcctac aaaaagccgt gggacccgtc cgcggcgatg
     6721 cggcagatgg ccaagtggga gggccatttc gacaaacgca tcttccacgc cttcgtcaag
     6781 gccgtgggca tctaccccgt cggctccttg gttcgcctgt cctctcagcg tctggccgtt
     6841 gtcgttgagc cgggaatgga atcactgctg actcccaagg ttcgcgtgtt cttctcgcta
     6901 cgctcgagag agccgatccc gatgcagacc atcgacctgg cggccacgag ttgcaaggac
     6961 agtatcactg gccccgaaga cccgacgctc tggaacttca agaacctcga cgacttgtgg
     7021 atggaatagc ccccacaaaa cgacggcccc gtgagggagc cgttgaacat cgtcgagcga
     7081 tgcccgtcca gggagggatg gccgagctcg attctccaga aggtagctgg tgacacgagt
     7141 gttgagccca cccaactagg gtgacggaaa tttctggggt tccggcttac atcccc
//
