LOCUS       Tn4658a                 5699 bp    DNA     linear       29-SEP-2020
DEFINITION  Pseudomonas putida.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Pseudomonas putida
  ORGANISM  Pseudomonas putida
            Unclassified.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn4658a
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Pseudomonas putida"
                     /mol_type="genomic DNA"
     repeat_region   1..38
                     /gene="IRL_Tn4658a"
     CDS             complement(34..3000)
                     /gene="tnpA"
                     /codon_start=1
                     /product="Tn4658a transposase"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLSLLRYPGYALGTDSELPEPVIQWVAKQVQADPESWAKYGERDVTRRE
                     HTQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLRTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSSAKHKHQQQFQKQGKAINDKVRLYSKIG
                     QALLEAKETGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRAAPAAQGVLAAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPE
                     GLDRRFYEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAI
                     NPSSDQYLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDSAVPNTAQALI
                     DQTSQLLPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLT
                     KMAESSPGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHTFAAHWGDGTTSSSDGQ
                     RFRAGGRGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPNSVQDYPTLRP
                     MVGGTLNIKHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERSTQAMGEAGKQVNGELLQYLSPLGWEHINLTGDYVWRQSR
                     RLEDGKFRPLRLPGKP"
     CDS             complement(3004..3564)
                     /gene="tnpR"
                     /codon_start=1
                     /product="Tn4658a resolvase"
                     /translation="MQGQRIGYVRVSSYDQNPERQLEQVEVGKLFTDKASGKDTQRPQ
                     LEAMLGFVREGDTVVVHSMDRLARNLDDLRRLVQKLTKRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRAAA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     misc_recomb     3635..3763
                     /gene="res"
     CDS             3906..5564
                     /gene="mcp"
                     /codon_start=1
                     /product="Methyl-accepting chemotaxis protein"
                     /translation="MSGWLARFSIKTRLIGLLVLLASMAVGLPMAGLNGMRTSNAVID
                     RLYNNMLLPIDKLGDINNHMHNARAQLLLALQHEPGSAFEKAHDHPSSMHFDRVDKSV
                     SIIRERLSDYQRIPMSKEQQRLSTELQNKLSDYLDNGVLPTLNAMRQGDYAGGNQILL
                     TRLDPAFMTTYASLRTLIESEASAAELSFQNADKQFERLVAMIGLALVVALSLVTVTA
                     LATVPSITKGVRELQVGGQQLADGNLGYRVTYQGRDELGEISSAFNEMASRFHHTFQE
                     LAGAVEQLAAAAEQTAQASSQTSDGIRRQQLETDLIATAMNEMSATVQDVAGNAASAA
                     RAAQEADQQSESGMRVVHQTIASIDSLASEVEHAATVIHELEADSAGISSVVDVIRSI
                     TEQTNLLALNAAIEAAHAGEHGRGFAVVADEVRSLASRTQQSANEIQAMIEKLQSGAN
                     RAVAVMESSCSKAQAGKQQVASAGHMLEQISNAVATINDMNAMIASAAEEQSSVAEEI
                     SRNVTNVSQIAEKTSEASRQNVATSTQLASLASQLQRLMHMFRL"
     repeat_region   5662..5699
                     /gene="IRR_Tn4658a"
ORIGIN      
        1 ggggagccta cagaattcgg aaaaaatcgt acgctaaggt tttccgggca accgtagcgg
       61 cctgaacttc ccgtcctcca gcctgcggct ctgccgccag acgtaatcgc cagtcaggtt
      121 gatgtgctcc cagcccagcg gcgacaggta ttgcagcaac tcgccattca cctgctttcc
      181 agcctcgccc attgcttggg tggagcgctc caggtacacc gtgttccaca gcacgatggc
      241 ggcggtcacc aagttgaggc cactggcccg gtagcgctgc tgttcgaaac tccgatccct
      301 gatctcgcct aggcggttga agaacaccgc cctggccagg gagttgcgcg cctcaccctt
      361 gttcagccct gcatgtacac ggcggcgcag ctcgacgctt tgcagccagt cgaggatgaa
      421 cagcgtgcgc tcgatccggc ccagctcgcg cagggccacg gccaggccgt tctggcgcgg
      481 atagctgccg agcttgcgca gcatcagaga ggcggtgacc gtgccctgct tgatcgagct
      541 ggccaggcgc aggatgtcgt cccaatgggc gcggacgtgc ttgatgttca gggtgccacc
      601 aaccattggg cgcaatgtcg ggtagtcctg gacgctattc ggaacataca gcttggtttc
      661 gccgaggtcg cggatgcgcg gtgcgaagcg gaagcccagc aggtgcatca gggcgaagac
      721 gtgatcggtg aaaccggccg tgtcggtgta gtgctcctcg atccgcaagt cggactcgtg
      781 gtacagcagg ccgtcgagca cataggtgga gtcacgcacg ccgacattca ccacgcgggt
      841 gctgaagggc gcgtactggt cggagatatg ggtgtagaac agccgccccg gctcgctgcc
      901 gtacttcggg ttgacgtgcc cggtgctttc gccccggccg cccgcccgga agcgctggcc
      961 atcggaagaa gaggtagtac cgtcgcccca gtgagcggcg aaggtatgac ggtactggtg
     1021 gttgaccagc tcggccaggg ccgccgagta ggtttcgtcg cggatgtgcc aggcttgcag
     1081 ccaggacagc ttggcgtagg tcaggccggg gctcgactcg gccatcttgg tcagcccgag
     1141 gttgatcgca tcgcccagga ttgctgacag cagcaatgtc cggtctttgg cctcggcacc
     1201 gtccttcagg tgggtgaagt ggcggctgaa acccgtccag tcgtccacgt ccatcagcag
     1261 ttcggtgatc ttgatgcgcg gcaacagctg actggtctgg tcgatcagcg cctgcgcggt
     1321 gttgggcacc gcagaatcca gcggggtgat tttcagcccg gactcggtga ggatggcatc
     1381 gggcagctcg ttgtccttgg ccagccgggt gacggtggcc aactgctcgt ccagcagctg
     1441 taaacgctct tccaggtact ggtcgctgct cgggttgatc gccaggggca gggcctgctc
     1501 gcgcttaagc gcggcgaact tctctgccgg cagcaggtag tcgtcgaagt cgcgaaactg
     1561 ccgcgagccc ttgacccaga tgtcgccgga acgcagggcg ttcttcagct cggacagcgc
     1621 acatatttca tagaaacgcc ggtcgaggcc ttccggggtg atcaccagcg gcttccagcg
     1681 cggcttgatg aaggctgtgg gtgcgtcggc tggcaccttg cgcaggttgt cggcgttcat
     1741 ttcgcgtagg gtctgcacag cggccagtac cccttgcgcg gccggcgcgg cgcgcagttc
     1801 cagcacctcc agcaaggccg gcgtgtagcg gcgcagggtg gcgaaattct cgccgactag
     1861 gtgcagatga tcgaagccct ccggtcgggc cagtagctca gcctcgctga cgctctcggt
     1921 gaactcgtcc caaggaatca ccgcctcgat ggcggcatag ggatcgctgc cggtttcctt
     1981 ggcctccaga agcgcctggc cgatcttgga gtacaggcgc accttgtcgt tgatcgcctt
     2041 gccctgcttc tggaactgct gctgatgctt gtgtttcgcg ctgctgaaca gcttgaccag
     2101 gatgcggtcg tgcagatcga ccaactcatc aatcacggtc gcggtgctct ccagcaccac
     2161 ggcggccagg gtcgcgtagc gccgctgcgg ctcgaacttg ccgaggtctt tgggcgtcat
     2221 ctgcccgccc tcgcgggcca gcttgagcag gcggttctgg tggatgtgcc ggcccaggcc
     2281 ttcgggcaaa tccaccagct gaaatgtcct cagccgctcg atgtgttcga gcatgtgccg
     2341 agagttcggc ttaagcggtg cctgcctcag ccaggtcaac caggtgatgc tgctgccggc
     2401 cttgagcttc aacagctcgt ccaacttggc ccgatgcgag tccgttagcg gctcgaccag
     2461 ggcgcgatag acccgccgat tggcccgcgc aatggcctcc gaacaagctc gatcaattac
     2521 gctcagcgcc ggcaggatgc gccgcttctg ccgcaaactc tccagcgcct gaccggccag
     2581 cagcaagccc ttgtcggtct gctgggccag ctcggtcaat tcgcgcacca gggcgcggaa
     2641 gtccgacagt ccgaacgggg ccagttgcag gtaggtgcga agttcctggg tatgctcgcg
     2701 acgggtcacg tcgcgctcgc cgtacttcgc ccagctctcc ggatcggcct gaacttgctt
     2761 agccacccac tggatgactg gctctggcag ctcgctgtcg gtgcccaacg catagccggg
     2821 gtaacgcaac aggctgagct gcaccgcaaa gcccagccgg ttggcgtcgc cgcgccgctg
     2881 acggatcagc gacaggtcgg agtcgttgaa ggtgtagtag cggatcaggt catcctggct
     2941 ttctggcaac gcaagcaggg tgtcccgctc cgtagccgag aggatcaagc gacgcggcat
     3001 gtatcagtcg tccgtgcgga ggtactggta gagggtttct cggctgatgt tgaattcgcg
     3061 ggcgagctgc gccttgggct cgcctgctgc tgctcgctgc cgcagggtag cagcctgttc
     3121 gtcggaaagg gccttcttac gaccccggta ggccccgcgc tgcttggcca gggcgatacc
     3181 ctcgcgctgc ctctcgcgga tcagggcgcg ctcgaactcg gcaaaggccc ccatgaccga
     3241 caacatgagg ttggccatgg gcgagtcctc accggtgaac accaagcctt ccttcaagaa
     3301 ctcgatgcgc acaccgcgct tggtcagctt ttgcaccagg cggcgcaggt catcgaggtt
     3361 gcgggcaagg cgatccatgc tgtgcaccac cacggtatcg ccctcacgga cgaagccgag
     3421 catggcctcc agctgggggc gctgggtgtc ctttcccgag gctttgtcgg tgaacagctt
     3481 gccgacctcg acctgctcaa gttggcgttc cgggttctgg tcgtaactgc tgacccggac
     3541 gtaaccgatg cgttgtccct gcaagatgcc tccatgggca ggatgggcgg cagggcttac
     3601 gttggttttt ggttccattg cgccctaagc cgtgaatttg tcaggctaaa atctatgacc
     3661 ttggcaagca ggtgtcaaag aatgcaaaag gcgactctat tctgacggcg tggcgcggcc
     3721 tttcctgaca tccagttagg gtatagccca acctgacggc ctttatgtct tgtttttgat
     3781 ctgagtacta atattgcccc agaccatatg gcgagaagcc ttgaggggga gcctgactca
     3841 agatttcaaa tgaaccagcc gatacaatga aggcacattg aaatatctaa taatgaggtg
     3901 acacgatgag tggttggcta gcccgtttct caataaaaac ccgtctcatc ggcttgctcg
     3961 tgcttctagc atctatggct gtcggcttgc caatggctgg tcttaatgga atgcgcacca
     4021 gcaatgcagt gattgatcgg ctgtacaaca acatgttgct accgatcgac aagcttggcg
     4081 acatcaataa ccacatgcac aacgcgcgtg ctcagctttt gcttgccctt cagcatgagc
     4141 ccggaagtgc cttcgagaag gcgcatgatc atccatcgag tatgcatttc gatagagtgg
     4201 ataaaagcgt aagcatcatc cgtgagcgcc tttctgacta ccagcgtata ccaatgagca
     4261 aagaacagca gcgccttagc actgagctgc agaacaaatt gtctgattac ttggataatg
     4321 gtgttctgcc gacgctcaat gcgatgcgcc aaggcgacta tgcgggaggg aaccaaatcc
     4381 tgctaacccg ccttgatcct gcttttatga ctacctatgc cagcttgcgc actcttattg
     4441 agtcggaggc gtcggcagct gaattaagct ttcaaaacgc ggataagcaa ttcgagcgtc
     4501 ttgtggcgat gataggactg gcgctggtag tggctctgtc tctggtcaca gtcacggctc
     4561 tggccactgt gccaagtatc accaaaggcg ttcgggagct tcaagtcggc ggtcagcagc
     4621 tcgctgatgg caatttgggc tatcgagtta cctatcaggg gcgtgatgag ctgggcgaaa
     4681 tatctagcgc attcaatgaa atggcttcgc gttttcacca cacctttcaa gagttggcag
     4741 gtgccgtgga gcaactggca gctgcagctg agcagacggc ccaggcaagt agtcaaacca
     4801 gtgacggtat ccgccgccag cagctggaga cagacctgat agctactgcg atgaacgaaa
     4861 tgtccgctac tgttcaggac gtagcgggga acgctgccag tgcggcacgg gcggcacagg
     4921 aggccgatca gcagtctgaa agcggcatgc gcgtggttca tcaaacaatc gcttctattg
     4981 acagcctcgc cagtgaagtt gaacacgctg ccactgtgat tcatgagctt gaggcagaca
     5041 gtgctggtat tagctctgta gtggatgtca ttcgaagcat taccgaacaa accaacctgc
     5101 ttgcccttaa cgcggctatt gaagctgcac atgctggtga gcacggacga ggctttgctg
     5161 ttgtcgcgga tgaggtgcgc tcgcttgctt cgcgcacgca acagtcagcc aacgaaattc
     5221 aggccatgat tgaaaagctg cagagcggcg caaatcgtgc tgttgctgtc atggaaagca
     5281 gttgcagcaa agcacaagct ggtaagcagc aggttgccag tgcaggccat atgctggagc
     5341 aaatcagtaa tgctgtggcg accattaatg acatgaatgc catgattgct tctgcagctg
     5401 aggagcaaag ctctgtcgcg gaggagatca gccgtaacgt taccaacgta agccagattg
     5461 ccgaaaaaac tagcgaagcc tcacgtcaga atgttgccac cagtacgcaa ttggcttctc
     5521 tggcaagcca acttcagcgt ctgatgcaca tgttccgtct ttagggaaac tctgaaaagc
     5581 cccagcttcg cggaattgat gcgctgtaac ccgctcggct gctgggctct ttttcccgag
     5641 aaaggacttt ctcaggccat ccttagcgta cgttattttc cgaattctgc gggctcccc
//
