LOCUS       Tn4451                  6338 bp    DNA     linear       29-SEP-2020
DEFINITION  Clostridium perfringens.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Unclassified.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn4451
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
     repeat_region   1..12
                     /gene="attL_Tn4451"
     CDS             76..2199
                     /gene="tnpX"
                     /codon_start=1
                     /product="Serine recombinase TnpX"
                     /translation="MSRTSRITALYERLSRDDDLTGESNSITNQKKYLEDYARRNGFE
                     NIRHFTDDGFSGVNFNRPGFQSLIKEVEAGNVETLIVKDMSRLGRNYLQVGFYTEVLF
                     PQKNVRFLAINNSIDSNNASDNDFAPFLNIMNEWYAKDTSNKIKAIFDARMKDGKRCS
                     GSIPYGYNRLPSDKQTLVVDPVASEVVKRIFTLANDGKSTRAIAEILTEEKVLTPAAY
                     AKEYHPEQYNGNKFTNPYLWAMSTIRNILGRQEYLGHTVLRKSVSTNFKLHKRKSTDE
                     EEQYVFPNTHEPIISQELWDSVQKRRSRVNRASAWGTHSNRLSGYLYCADCGRRMTLQ
                     THYSKKDGSVQYSYRCGGYASRVNSCTSHSISTDNVEALILSSVKRFSRFVLNDEQAF
                     ALELQSLWNEKQEEKPKHNQSELQRCQKRYDELSTLVRGLYENLMSGLLPERQYKQLM
                     KQYDDEQAELETKMETMKTELAEEKVSSVDIKHFISLIRKCKNPTEISDTMFNELVDK
                     IVVYEAEGVGKARTQKVDIYFNYVGQVDIAYTEEELAEIETQKEQEEQQRLARQRKRE
                     KAYREKRKAQKIAENGGEIVKTKVCPHCNKEFIPTSNRQVFCSKECCYQARQDKKKTD
                     REAERGNHYYRQRVCAVCGNSYWPTHSQQKFCSEECQRVNHNKKTLEFYHHKKEKEKL
                     QCKDLSQTKERVSDMNLSGTITTPA"
     CDS             2124..2495
                     /gene="tnpV"
                     /codon_start=1
                     /product="Transposon-encoded protein TnpV"
                     /translation="MQRFITDERTGIRYELIGDYYYPCLTAEEKPLLSRYGRMRERYL
                     KEHKRVLYYTLMTSGKLYEHLAEIDTSACDMAEYLIKEMARKQGVTEQLKAVDMMRWI
                     GLMNNIRACVDEIVLNDIVYS"
     CDS             2677..3300
                     /gene="catP"
                     /codon_start=1
                     /product="Chloramphenicol acetyltransferase"
                     /translation="MVFEKIDKNSWNRKEYFDHYFASVPCTYSMTVKVDITQIKEKGM
                     KLYPAMLYYIAMIVNRHSEFRTAINQDGELGIYDEMIPSYTIFHNDTETFSSLWTECK
                     SDFKSFLADYESDTQRYGNNHRMEGKPNAPENIFNVSMIPWSTFDGFNLNLQKGYDYL
                     IPIFTMGKYYKEDNKIILPLAIQVHHAVCDGFHICRFVNELQELINS"
     CDS             3549..4571
                     /gene="tnpY"
                     /codon_start=1
                     /product="Transposon-encoded protein TnpY"
                     /translation="MKLNENEMNFSVPLEIIKASEIEPKEVKWLWYPYIPFGKVTLLQ
                     GDPGDGKSKLMLSIAALLSKGEPIPFTETEENEPMTIIYQTTEDDADDTVVPRFNSAG
                     GDGENLIFIKEDEKTLSFGDNRIREAIEKYNAKLLILDPLSSYIGENCSMNNANETRA
                     EFNHLIAVAKDTGCAIVIIAHMNKMKDNNPLYRTNGSIDIAGAARSILAITRTPNKEA
                     PAERYLVQVKSNLAPTGSAILFEVAEKGVNFISEMEMTAEEAFLSLAPQIGRPNEKEM
                     KAKSFLIEMLQGGEMLSSDCEEKLEAAGFKKSTIKKAKKNAGVISKKKGFLWYWSLPM
                     SDIPRE"
     misc_recomb     4677..4701
                     /gene="oriT"
     CDS             4752..6017
                     /gene="rlx"
                     /codon_start=1
                     /product="Relaxase"
                     /translation="MAKNNKADMSCARVKKYTASDVSKAERHNERKNETYENMNVIEE
                     RIPYNVHFKKPFAPTYMEQLKQMETDGMVSLRGLRKDATLFNEIVIDVNTMYFERNGG
                     YEYAKQFYEEAFHFIEEKFGADNVISAVMHADEINVAATEELGKEVYHYHLHAMVLPV
                     VEKEILWSKRCKDEKLRGTVKEVVHQISHSKKWKSDIPLTDEKGNPLLRKNGKPMFRA
                     SYSILQDELFNHMTEQGFKGFQRGEYGSTAEHLTSLQYQIKQDKERLEKLQKRIQREQ
                     IKYEPARNVSKTYNEIDRMGQKTITGKMAISKEDYSELTALAKEGITSRAEISELEQS
                     ANYYRQKYFDSANALERMKTKYDELKEKCRPFLQALEHFPEVAKLFTEKVKQLFSFKE
                     AQERAEKEAREKERQERIKARRNKRGMER"
     CDS             6137..6325
                     /gene="tnpW"
                     /codon_start=1
                     /product="Transposon-encoded protein TnpW"
                     /translation="MKEQKHSYSQCIGKTTFIVNVKQSESAKKPLNTVFQDICKHEVL
                     GDFFTDKSFNLENPQKVS"
     repeat_region   6327..6338
                     /gene="attR_Tn4451"
ORIGIN      
        1 gggctatact ttaataggac aaaaaaatta actgtcctaa attgaaaaaa tacatacgga
       61 aaggaggata agaaaatgtc aaggacttca agaattacag cactttacga gcgtttgtca
      121 agagatgatg accttactgg cgagagtaat tctattacca atcaaaagaa atacctcgaa
      181 gattatgccc gtaggaatgg ttttgagaac attcgccatt ttaccgatga cggattttcg
      241 ggtgtaaatt tcaatcgccc tggctttcaa tctctgataa aagaagttga agcaggaaat
      301 gtagaaacct tgattgttaa ggatatgagc cgattggggc gaaattatct gcaagtaggt
      361 ttttatacgg aagttctgtt tccacagaaa aatgtccgtt tccttgcaat taacaacagt
      421 attgacagta acaacgcttc ggataatgac tttgctccgt ttttgaatat tatgaacgaa
      481 tggtatgcca aagacacaag caacaaaatc aaggctatat tcgatgcccg tatgaaagac
      541 ggaaagcgtt gtagcggttc tatcccttat gggtataacc gactgccgag cgacaaacaa
      601 acgcttgtgg ttgaccctgt ggcttcggaa gtggtaaagc gtatctttac tcttgccaat
      661 gatggcaaaa gtacaagggc aatcgcagaa atactgaccg aagaaaaagt tttaacccct
      721 gcggcatacg caaaggaata ccaccccgaa cagtacaacg gcaacaagtt cacaaaccct
      781 tatctttggg caatgtcaac gataagaaat attttaggca ggcaggaata tctcggtcac
      841 accgttttgc gaaagtcggt aagcacaaat ttcaaacttc acaagagaaa aagcacagac
      901 gaagaagaac agtatgtatt tccgaataca cacgagccta tcatatcgca ggaactttgg
      961 gacagcgttc aaaaacgcag aagcagagta aatcgtgcct cggcttgggg aacgcacagc
     1021 aaccgtttaa gcggatattt gtactgtgcc gattgcggaa gaagaatgac tttgcagaca
     1081 cattacagca aaaaagacgg ttctgtgcag tattcttacc gttgcggtgg gtatgcaagc
     1141 agagtgaaca gttgtaccag tcattcgatt agtaccgata atgttgaagc cttgatatta
     1201 tcatctgtca aacgcttttc aaggtttgtt ctgaatgatg aacaagcatt tgctttggaa
     1261 ctgcaatctc tttggaatga aaaacaggag gaaaagccga aacacaatca atcggaactg
     1321 caacgctgtc agaaacgcta tgacgaactc tctacccttg ttcgtggctt gtatgaaaat
     1381 cttatgtcgg gattactgcc cgaaagacag tataagcaac tgatgaaaca gtatgatgac
     1441 gagcaggcag agttggaaac gaaaatggaa acgatgaaaa cagaacttgc cgaagaaaaa
     1501 gtaagttccg ttgatattaa gcatttcatt tcgctgatac gcaagtgtaa aaatcctacg
     1561 gaaatctccg atacaatgtt taatgaactt gttgataaga tagtggttta tgaagcagag
     1621 ggtgtgggaa aagcacgaac acaaaaggtc gatatttatt ttaactatgt cggtcaagtg
     1681 gatattgcct ataccgaaga agaacttgcc gagatagaaa cacagaaaga gcaggaggaa
     1741 cagcaacgct tggcaagaca gcgcaagcgt gaaaaagcct accgagaaaa gcgaaaggca
     1801 cagaaaatcg ctgaaaacgg tggcgaaatc gttaagacaa aggtttgccc tcattgcaac
     1861 aaagagttta tcccgacaag caaccgacag gtgttctgtt ccaaagagtg ctgctatcaa
     1921 gcaaggcaag acaaaaagaa aacagaccga gaagcagaac gaggaaatca ctattaccga
     1981 cagcgtgtat gtgctgtgtg cggcaattcc tattggccta cacacagcca acagaaattc
     2041 tgctccgaag aatgtcaaag ggtaaatcac aataagaaaa cattggaatt ttaccaccat
     2101 aaaaaagaaa aggagaagct gcaatgcaaa gatttatcac agacgaaaga acgggtatcc
     2161 gatatgaact tatcggggac tattactacc cctgcttaac cgcagaagaa aaacctttgc
     2221 tttcaaggta cggaagaatg cgagagcgat atttgaaaga acacaagcga gttttatatt
     2281 acactctgat gacaagtgga aagttatatg aacaccttgc cgagattgac acttcggctt
     2341 gtgatatggc tgaatatctc ataaaggaaa tggcaagaaa gcaaggcgtt accgagcaat
     2401 taaaggctgt ggatatgatg agatggatag gattgatgaa caatatcaga gcttgcgtag
     2461 atgaaatcgt attaaacgat attgtgtatt cataacggaa tactatccga aagagaactg
     2521 ctgtcaagtg atggcagttt ttctttttcg gcaagtgttc aagaagttat taagtcggga
     2581 gtgcagtcga agtgggcaag ttgaaaaatt cacaaaaatg tggtataata tctttgttca
     2641 ttagagcgat aaacttgaat ttgagaggga acttagatgg tatttgaaaa aattgataaa
     2701 aatagttgga acagaaaaga gtattttgac cactactttg caagtgtacc ttgtacatac
     2761 agcatgaccg ttaaagtgga tatcacacaa ataaaggaaa agggaatgaa actatatcct
     2821 gcaatgcttt attatattgc aatgattgta aaccgccatt cagagtttag gacggcaatc
     2881 aatcaagatg gtgaattggg gatatatgat gagatgatac caagctatac aatatttcac
     2941 aatgatactg aaacattttc cagcctttgg actgagtgta agtctgactt taaatcattt
     3001 ttagcagatt atgaaagtga tacgcaacgg tatggaaaca atcatagaat ggaaggaaag
     3061 ccaaatgctc cggaaaacat ttttaatgta tctatgatac cgtggtcaac cttcgatggc
     3121 tttaatctga atttgcagaa aggatatgat tatttgattc ctatttttac tatggggaaa
     3181 tattataaag aagataacaa aattatactt cctttggcaa ttcaagttca tcacgcagta
     3241 tgtgacggat ttcacatttg ccgttttgta aacgaattgc aggaattgat aaatagttaa
     3301 cttcaggttt gtctgtaact aaaaacaagt atttaagcaa aaacatcgta gaaatacggt
     3361 gttttttgtt accctaaaat ctacaatttt atacataacc acaggttagt acaaagacct
     3421 tgtgtttctt tttgaaaggc ttaaaacaag gatttttcct tgatttaagc cccgaaaagc
     3481 aacacaacca aggttttagt atcaatctgt ggtttttata ttttcagaga aaaggagaac
     3541 aagaaaaaat gaaactaaat gaaaacgaaa tgaatttcag cgtacctctt gaaatcatca
     3601 aggcaagtga aatcgagccg aaagaagtaa agtggctgtg gtatccgtat attccgtttg
     3661 gcaaagttac gctattgcag ggagatccgg gcgatgggaa aagcaagtta atgctttcca
     3721 tcgccgccct gctctcaaag ggcgaaccta tcccctttac cgaaacggaa gaaaatgagc
     3781 ctatgactat catctatcag acaacggaag atgacgcaga cgataccgta gtaccccgct
     3841 tcaattctgc cggcggagac ggagaaaatc ttatcttcat caaggaagat gaaaagactt
     3901 tatctttcgg agataaccgc attcgtgaag cgattgaaaa gtacaatgca aagcttctaa
     3961 ttcttgaccc gttgagttcg tatatcggag agaattgctc tatgaataat gccaacgaaa
     4021 cacgagcgga gtttaaccat ctgatagcgg ttgcaaaaga tacgggttgt gcgattgtca
     4081 ttatcgctca tatgaacaag atgaaagata ataatccact ctaccgcacc aacggctcga
     4141 ttgacattgc gggtgctgca agaagcattc ttgcaatcac acgcacaccg aacaaggaag
     4201 cacctgcgga aagatatttg gtgcaagtga aatcaaatct tgctccgaca ggttcggcaa
     4261 ttctttttga ggttgccgag aaaggtgtga acttcatttc cgaaatggaa atgacagccg
     4321 aagaagcgtt tttatctctt gccccacaaa taggcagacc gaatgaaaaa gagatgaaag
     4381 caaaatcatt tctcattgaa atgttgcagg gcggagaaat gctttcttcg gattgtgaag
     4441 aaaaacttga agccgcaggg ttcaagaaat cgaccatcaa aaaggcaaag aaaaacgcag
     4501 gagttatctc caagaagaaa ggttttcttt ggtattggtc tttgccgatg agcgatatac
     4561 cgagagaata aaaacagaat gcctgtcggt gggacagtct gtttttgtga ttgaggtatc
     4621 aaaggaggtc aagggggaac acccttgccc acgacatctt tgcagacgaa gtctgaaagt
     4681 gtcatagtgg gttacacaca ttcaaagaat gttgtgtaat ggcttcgccc tgccgagaaa
     4741 ggagagcaaa aatggcgaaa aacaacaagg cggatatgag ttgtgcaagg gttaaaaagt
     4801 acaccgcttc tgatgtgagc aaggcagaaa ggcacaacga acgcaagaat gaaacctatg
     4861 aaaatatgaa tgtgattgag gaacgcatac cctataatgt gcatttcaaa aagccttttg
     4921 ccccgaccta tatggaacaa ttaaagcaga tggaaactga cggtatggtg tcgcttcgag
     4981 ggctgaggaa agacgcaacg ctgttcaatg agattgtgat tgatgtgaac acaatgtact
     5041 tcgagcgtaa cggcggctac gaatatgcaa agcagtttta cgaagaagca ttccatttta
     5101 tcgaggaaaa attcggtgct gataatgtca tatcggctgt tatgcacgct gatgaaatca
     5161 atgtagcggc aaccgaggaa cttggaaaag aggtttacca ctatcatctt cacgctatgg
     5221 ttttgcctgt ggtggagaaa gaaatcctat ggagcaagcg ttgcaaagat gaaaaactgc
     5281 gaggaacggt taaggaagtt gttcatcaaa tcagccattc aaagaaatgg aaatcggata
     5341 ttccgctgac cgacgaaaaa ggcaatccgc ttctcagaaa gaacggaaaa ccgatgttcc
     5401 gagcttcata cagtattctg caagatgaat tgtttaacca tatgacggag caaggcttca
     5461 aaggctttca gcgtggcgaa tatggaagta cagcggaaca tcttacttcc ctacaatatc
     5521 aaatcaagca ggacaaggaa cgattagaga agttgcagaa gcgtattcag cgtgaacaga
     5581 taaagtatga gcctgcccgt aatgtttcca aaacctataa cgagattgac cgtatggggc
     5641 agaaaacaat cacgggtaaa atggcaatct ccaaagagga ttattctgag cttactgccc
     5701 ttgctaaaga gggcattacc agtcgtgcgg aaatcagcga attagaacag agtgcaaact
     5761 attaccgaca gaaatatttt gacagtgcaa acgcacttga aagaatgaaa accaaatacg
     5821 acgaactgaa agaaaagtgc agaccttttc ttcaagcgtt ggagcatttc cccgaagttg
     5881 ctaaactttt taccgagaaa gtgaagcaac ttttttcttt taaggaagcg caggaacgag
     5941 ccgaaaaaga agcaagggaa aaagaacgac aggagcgtat caaggctcga agaaacaagc
     6001 gtggtatgga aagataacct tttccattat actcacgact taatgacttg ttgatatatt
     6061 gaaaatctgc ggttgatggg tgttctaatg tgaataccca tcaatcaaaa ttttatgaaa
     6121 gagaggaacg gactatatga aagaacagaa acattcttat tcacaatgca ttggaaagac
     6181 aacattcatc gtaaatgtaa agcagtctga aagtgccaaa aagcctttga atacggtgtt
     6241 tcaagacatc tgcaagcacg aggttttggg cgatttcttt acggacaaat cgtttaattt
     6301 agaaaatcca caaaaagtat cttgacaaac tcgacccc
//
