LOCUS       Tn402                   7400 bp    DNA     linear       29-SEP-2020
DEFINITION  Klebsiella aerogenes.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Unclassified.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn402
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
     repeat_region   1..25
                     /gene="IRL_Tn402"
     repeat_region   9..27
     repeat_region   49..67
     repeat_region   78..96
     repeat_region   110..128
     CDS             142..1821
                     /gene="tniA"
                     /codon_start=1
                     /product="Tn402 transposase"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVVTLEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLV
                     DFLPILRRTLTRTGFVIDHIHYYADALKPWIARRERWPSFLIRRDPRDISRIWVLEPE
                     GQHYLEIPYRTLSHPAVTLWEQRQALAKLRQQGREQVDESALFRMIGQMREIVTSAQK
                     ATRKARRDADRRQHLKTSARPDKPVPPDTDIADPQADNLPPAKPFDQIEEW"
     CDS             1824..2732
                     /gene="tniB"
                     /codon_start=1
                     /product="Tn402 transposition regulator"
                     /translation="-
                     DEYPIIDLSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVEALNRLEALYAWPNKQ
                     RMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQMPSEPSVIRFYVALL
                     AAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAGNSVNRREFLNLLRFL
                     GNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDDCCSLLASFAASLPLR
                     RPSPIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEAINHRTLSMAVYTGPS
                     ERRRQFERELM"
     CDS             2729..3946
                     /gene="tniQ"
                     /codon_start=1
                     /product="Tn402 target-site selection protein"
                     /translation="-
                     KPAPRWPLHPAPKEGEALSSWLNRVALCYHMEEPDLLEHDLGHGQVDDLDTAPPLSLL
                     ALLSQRSGIELDRLRCMSFAGWVPWLLDSLDDQIPDALETYAFQLSVLLPRLRRKTRS
                     ITSWRAWLPSQPINRACPLCLSDPENQAVLLAWKLPLMLSCPLHGCWLESYWGVPGRF
                     LGWENADAEPRTASDAIAAMDQRTWQALTTGHVELPRRRIHAGLWFRLLRTLLDELNT
                     PLSACGTCAGYPRQVWEGCGHPLRAGQSLWRPYETLNPIVRLQMLEAAATAISLIEVR
                     DISPPGEQAKLFWSEPQTGFTSGLPTKAPKPEPINHWQRAVQAIDEAIIEARHNPETA
                     RSLFALASYGRRDPASLERLRATFVKEGIPPEFLSHYLPDAPFACLKQNDGLSDKF"
     misc_recomb     3970..3999
                     /gene="res"
     CDS             4008..4631
                     /gene="tniR"
                     /codon_start=1
                     /product="Tn402 resolvase"
                     /translation="-
                     LIGYMRVSKADGSQSTNLQRDALIAAGVSLAHLYEDLASGRRDDRPGLAACLKALREG
                     DTLIVWKLDRLGRDLRHLINTVHDLTARSVGLKVLTGHGAAVDTTTAAGKLVFGIFAA
                     LAEFERELISERTVAGLISARARGRKGGRPFKMTAAKLRLAMASMGQPETKVGDLCEE
                     LGITRQTLYRHVSPKGELRPDGVKLLSLGSAA"
     CDS             complement(4880..5212)
                     /gene="qacE"
                     /codon_start=1
                     /product="Quaternary ammonium compound efflux SMR
                     transporter QacE"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIVSGVVVLN
                     LLSKASAH"
     CDS             complement(5317..5607)
                     /gene="orf291"
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MFIQTAFSFSNAVQRWVCRFSGLRLVALRWFVVFLASGPCVASA
                     SSYFFRSAVPPRWHRAFSQLAPVAKLRLSVLASGSNISVKPTRILRSAYLAR"
     CDS             complement(5744..5980)
                     /gene="dhfrIIc"
                     /codon_start=1
                     /product="Dihydrofolate reductase type IIc"
                     /translation="MDQHNNGVSTLVAGQFALPSHATFGLGDRVRKKSGAAWQGQVVG
                     WYCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     CDS             6185..7198
                     /gene="intI1"
                     /codon_start=1
                     /product="IntI1 integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVNWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     repeat_region   7351..7369
     repeat_region   7374..7392
     repeat_region   7376..7400
                     /gene="IRR_Tn402"
ORIGIN      
        1 tgtcattttc agaagacgac tgcaccagtt gattgggcgt aatggctgtt gtgcagccag
       61 ctcctgacag ttcaatatca gaagtgatct gcaccaatct cgactatgct caatactcgt
      121 gtgcaccaaa gcgaggtgag catggcgacg gacaccccac ggattccaga acaaggcgtg
      181 gccactctgc ctgatgaggc ttgggagcgt gcgcgccgtc gtgcggagat catcagtccg
      241 ttggcgcagt cggagacggt cgggcacgaa gcggccgata tggcggctca ggcgctgggc
      301 ttgtctcggc gccaggtata cgttctgatc cggcgtgccc ggcaaggcag cggcctcgtg
      361 acggatctgg tgcccggcca gtccggtgga ggtaaaggta aggggcgctt gccggaaccg
      421 gtcgagcgcg tcatccacga gctactgcaa aagcggttcc tgaccaagca gaagcgcagc
      481 ctagcggcct ttcaccgcga agtcactcag gtgtgcaagg ctcaaaaact gcgagtgccg
      541 gcgcgcaata ccgtggcctt acggatcgct agccttgacc cgcgcaaggt catccgccgg
      601 cgggaaggcc aggatgccgc tcgtgaccta caaggtgtgg gcggcgagcc tcctgccgtg
      661 accgcgccgc tggagcaggt gcagatagac catacggtca tcgacctgat cgtggtcgat
      721 gaccgcgacc ggcaacctat tggccgcccg tacctgaccc tcgccatcga cgtgttcacc
      781 cgctgcgtgc tcggcatggt cgtcacgctg gaagcgccgt ctgccgtttc ggttggcctg
      841 tgcctcgtgc atgtcgcctg cgacaagcgc ccttggctgg aaggactgaa cgtggaaatg
      901 gattggcaga tgagcggcaa gcccttgctg ctctacctag acaacgcggc cgagttcaag
      961 agcgaggccc tgcgccgggg ttgcgagcag catggcatcc ggctggacta tcgcccgctg
     1021 ggacagccgc actatggcgg catcgtggaa cggatcatcg gcacggcgat gcagatgatt
     1081 cacgacgaac tgccgggaac gaccttctcc aaccctgacc agcgcggcga ctacgattcc
     1141 gaaaacaagg ccgccctgac gctgcgcgag ctagagcgct ggctcacatt ggcggtcggc
     1201 acctaccacg gttcggtgca caacggcctg ctccaaccgc cggccgcgcg ctgggccgag
     1261 gccgtggcgc gtgtcggcgt accggccgtc gtcacacgcg ctacttcgtt cctggtcgat
     1321 tttctgccga tcctccggcg cacgctgacc cgcaccggct ttgtcatcga ccacatccac
     1381 tactacgccg atgcgctcaa gccgtggatt gcgcggcgtg aacgctggcc gtcctttctg
     1441 atccggcgcg atccgcgcga catcagccgt atctgggtcc tggaaccgga gggacagcat
     1501 tacctggaaa ttccctaccg taccttgtcg catccggctg tcaccctctg ggaacaacgg
     1561 caggcgctgg cgaaactgcg gcagcaaggg cgcgaacagg tggatgagtc ggcgctgttc
     1621 cgcatgatcg gccagatgcg tgagattgtg accagcgcgc agaaggccac acgcaaggcg
     1681 cggcgtgacg cggatcgccg ccagcacctc aagacatcag ctcggccgga caagcccgtt
     1741 ccgccggata cggatattgc cgacccgcag gcagacaact tgccacccgc caaaccgttc
     1801 gaccagattg aggagtggta gccgtggacg aatatcccat catcgacctg tcccacctgc
     1861 tgccggcggc ccagggcttg gcccgtcttc cggcggacga gcgcatccag cgccttcgcg
     1921 ccgaccgctg gatcggctat ccgcgcgcag tcgaggcgct gaaccggctg gaagcccttt
     1981 atgcgtggcc aaacaagcaa cgcatgccca acctgctgct ggttggcccg accaacaatg
     2041 gcaagtcgat gatcgtcgag aagttccgcc gcacccaccc ggccagctcc gacgccgacc
     2101 aggagcacat cccggtgttg gtcgtgcaga tgccgtccga gccgtccgtg atccgcttct
     2161 acgtcgcgct gctcgccgcg atgggcgcgc cgctgcgccc acgcccacgg ttgccggaaa
     2221 tggagcaact ggctctggca ctgctgcgca aggtcggcgt gcgcatgctg gtgatcgacg
     2281 agctgcacaa cgtgctggcc ggcaacagcg tcaaccgccg ggaattcctc aacctgctgc
     2341 gcttcctcgg caacgaactg cgcatcccgt tggttggggt aggcacgcgc gacgcctacc
     2401 tagccatccg ctccgatgac cagttggaaa atcgcttcga gccgatgatg ctgccggtat
     2461 gggaggccaa cgacgattgc tgctcactgc tggccagctt cgccgcttcg ctcccgctgc
     2521 gccggccttc cccaattgcc acgctggaca tggctcgcta cctgctcaca cgcagcgagg
     2581 gcaccatagg ggaactggcg cacttgctga tggcggcggc catcgtcgcc gtggagagcg
     2641 gcgaggaagc gatcaaccat cgcacactca gcatggccgt ttacaccgga cccagcgagc
     2701 ggcggcggca attcgagcgg gaactgatgt gaagcctgcg ccgcgctggc cgctgcatcc
     2761 cgccccgaaa gaaggcgagg cgctgtcctc atggctcaac cgcgtggccc tttgctatca
     2821 catggaggag cccgacctgc tggagcacga tcttggtcac ggccaggtcg atgacctgga
     2881 caccgcgcca ccactctcgc tgctggcgtt gctttcccag cggagcggca tcgagctgga
     2941 ccggctgcgc tgtatgagtt tcgccggatg ggtgccttgg ctactggaca gccttgatga
     3001 ccagattcca gacgccttgg aaacctatgc gtttcagctc tcggtgttgc tgccaagact
     3061 ccgccgtaag acgcgatcca tcacgagctg gcgtgcctgg ctgcccagcc agccgataaa
     3121 ccgcgcctgt ccgctctgcc tgagcgatcc ggagaaccaa gccgtactgc tcgcgtggaa
     3181 gctgcccctg atgctgagct gcccgctgca tggctgctgg ctggaatcct attggggcgt
     3241 gccagggcgg tttctcggtt gggagaacgc cgacgccgaa ccgcgcaccg ccagcgacgc
     3301 gattgcggcg atggaccagc gtacctggca ggcactgaca accggtcacg tggagctgcc
     3361 gcgccgacgc atccacgccg gattgtggtt tcgacttctt cgcacgctgc tcgatgagct
     3421 gaacaccccg ctttccgcgt gcggaacctg cgcggggtat ccccgccaag tctgggaagg
     3481 ctgcgggcat ccgctgcgtg ctgggcaaag tctgtggcga ccgtatgaaa ccctgaatcc
     3541 gatagtacgg ttacagatgc tggaggcggc ggcaacggca atcagcttga ttgaggtgag
     3601 ggacatcagc ccgccaggcg agcaggcaaa gctattctgg tccgagcccc aaaccgggtt
     3661 caccagtggc ctgccgacga aagcgccgaa gcccgagccc atcaatcact ggcagcgtgc
     3721 agtccaggcc atcgacgagg ccatcattga agcgcgacac aaccccgaga cggcacgctc
     3781 gctgttcgcg ttggcttcct atggtcggcg cgatcccgct tccttggaac ggttgcgcgc
     3841 caccttcgtg aaggaaggca tcccgccgga atttctgtca cattacctgc ctgatgcacc
     3901 ctttgcatgt cttaaacaaa atgacgggtt aagtgacaaa ttttgacgga tagagctttc
     3961 cggctcacac tgtcacataa tcgaacgtat acgtgacggg tgaaaaggtg ctgatcggct
     4021 acatgcgggt atcgaaggcg gacggatccc agtccaccaa tttgcaacgc gatgcgctca
     4081 tcgccgctgg tgtgagcctt gcgcaccttt acgaggatct ggcctcgggc aggcgcgatg
     4141 atcgcccagg gttggctgct tgcctgaagg cgcttcgtga aggggacacg ctgatcgtgt
     4201 ggaagctcga tcggcttggc cgtgatctgc gccacctgat caacaccgtg cacgacctaa
     4261 ctgcgcgtag cgtgggcctg aaggtcctga ccggtcacgg tgcggcggtc gacacgacga
     4321 ctgccgccgg caagcttgtg ttcggtattt ttgccgcgct ggccgagttc gagcgtgagt
     4381 tgatttccga gcgaacagtc gctggactta tctcggcgcg cgctcgcggc aggaaagggg
     4441 ggcgcccctt caagatgacc gccgccaagc tacgcctggc gatggccagc atggggcaac
     4501 cggaaaccaa ggtgggcgat ctctgcgaag aactcgggat tacccggcag acgctctacc
     4561 ggcacgtgtc gcccaagggc gaactgcggc cagacggcgt aaagctgctc tccctcggtt
     4621 cagccgcata aatggaggcg acctggaacg gggcgctgtt cagtgcggca acgatccgat
     4681 taccggtgtc gacccagagc agccgtagag cttttgggaa agctgtcgtt caacgccgag
     4741 ttcagcggca gtttttaagt tgtgatttta tggaatactt ttgcgcagca aaaccataaa
     4801 gccgcgactt aaaaactgtc cagcgcaggc acgaagtgct ggagcggtgc tgcaacgact
     4861 tgttagatga ctgagtttat tagtgggcac ttgctttgga aagcaagttt aaaactacta
     4921 caccactaac tatgagcccc atacctacaa agccccacgc atcaagcttt tgcccatgaa
     4981 gcaaccaggc aatggctgta attatgacga cgccgagtcc cgaccagact gcataagcaa
     5041 caccgacagg gatggatttc agaaccagag aaagaaaata aaatgcgatg ccataaccga
     5101 ttatgacaac ggcggaaggg gcaagcttag taaagccctc gctagatttt aatgcggatg
     5161 ttgcgattac ttcgccaact attgcgataa caagaaaaag ccagcctttc atgatatatc
     5221 tcccaatttg tgtagggctt attatgcacg cttaaaaata ataaaagcag acttgacctg
     5281 atagtttggc tgtgagcaat tatgtgctta gtgcatctaa cgggcgaggt aagccgaccg
     5341 cagaatgcgg gtcggcttga ccgaaatgtt agagccagaa gccaaaacgg ataaccgtaa
     5401 cttggcgacg ggcgctaact gcgaaaaggc acggtgccac cgaggcggca cagcactacg
     5461 aaaaaaatag ctgctcgcgc ttgctacaca agggccagag gccaaaaaga ccacaaacca
     5521 gcgtaacgcc accaggcgaa gcccagagaa acggcaaacc cagcgctgca cagcgtttga
     5581 gaaactgaat gccgtttgaa taaacatggg cgaaataaag gagggtttca gtggtactaa
     5641 cgctctagct cagcgggcgg cgaagccgtc cgctggagcg accagttggg ctgtgggcgg
     5701 tactgcacgg cttgagcgct gagcggtgca ctcaaccgtg aattcaggcc acgcgttcaa
     5761 gcgcagccac aggataaatc tgtactgaac cggggtgaga ctcggactcg acggcatagc
     5821 cttcaggggt cagttttgtg cagtaccacc cgacaacttg accctgccaa gcggcgccag
     5881 atttcttgcg cacgcgatct cccaggccaa acgtggcgtg cgatgggagc gcaaactggc
     5941 cagcaactag agtactgact ccattgttgt gttggtccat atctgacctt tcttgtgaat
     6001 ttcttgagat gcttgctagc ccgacaacgc tgcgtcggtg aagcccaact ttgttttagg
     6061 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
     6121 gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca
     6181 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
     6241 gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa
     6301 ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag
     6361 cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca
     6421 tcgtcaggca ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc
     6481 ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc
     6541 ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagct
     6601 tctgtatgga acgggcatgc ggatcagtga gggtttgcaa ctgcgggtca aggatctgga
     6661 tttcgatcac ggcacgatca tcgtgcggga gggcaagggc tccaaggatc gggccttgat
     6721 gttacccgag agcttggcac ccagcctgcg cgagcagctg tcgcgtgcac gggcatggtg
     6781 gctgaaggac caggccgagg gccgcagcgg cgttgcgctt cccgacgccc ttgagcggaa
     6841 gtatccgcgc gccgggcatt cctggccgtg gttctgggtt tttgcgcagc acacgcattc
     6901 gaccgatcca cggagcggtg tcgtgcgtcg ccatcacatg tatgaccaga cctttcagcg
     6961 cgccttcaaa cgtgccgtag aacaagcagg catcacgaag cccgccacac cgcacaccct
     7021 ccgccactcg ttcgcgacgg ccttgctccg cagcggttac gacattcgaa ccgtgcagga
     7081 tctgctcggc cattccgacg tctctacgac gatgatttac acgcatgtgc tgaaagttgg
     7141 cggtgccgga gtgcgctcac cgcttgatgc gctgccgccc ctcactagtg agaggtaggg
     7201 cagcgcaagt caatcctggc ggattcacta cccctgcgcg aaggccatcg gtgccgcatc
     7261 gaacggccgg ttgcggaaag tcctccctgc gtccgctgat ggccggcagc agcccgtcgt
     7321 tgcctgatgg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
     7381 gccgtcttct gaaaacgaca
//
