LOCUS       Tn4003                  4717 bp    DNA     linear       29-SEP-2020
DEFINITION  Staphylococcus aureus.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Unclassified.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn4003
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
     repeat_region   complement(1..27)
                     /gene="IRR_IS257R2"
     CDS             complement(59..733)
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS257R2 transposase"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRGRGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDI
                     WLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCHCTSK
                     YLNNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEI
                     SIMLAS"
     repeat_region   complement(763..789)
                     /gene="IRL_IS257R2"
     CDS             847..1803
                     /gene="thyE"
                     /codon_start=1
                     /product="Thymidylate synthase"
                     /translation="MYNPFDEAYHGLCEEILEIGNRRDDRTHTGTISKFGHQLRFDLT
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYVQSDDYHGP
                     DMTDFGHRSQQDPEFNEQYKEEMKKFKERILNDDAFAKKYGNLGNVYGKQWRDWEDKN
                     GNHYDQLKSVIQQIKTNPNSRRHIVSAWNPTEIDSMALPPCHTMFQFYVQEGKLNCQL
                     YQRSADIFLGVPFNIASYALLTHLVAKECGLEVGEFIHTFGDAHIYSNHMDAIHTQLS
                     RDSYLPPQLKINTDKSIFDINYEDLELINYESHPAIKAPIAV"
     CDS             1845..2330
                     /gene="dfrC"
                     /codon_start=1
                     /product="Dihydrofolate reductase"
                     /translation="MTLSIIVAHDKQRVIGYQNQLPWHLPNDLKHIKQLTTGNTLVMA
                     RKTFNSIGKPLPNRRNVVLTNQASFHHEGVDVINSLDEIKELSGHVFIFGGQTLYEAM
                     IDQVDDMYITVIDGKFQGDTFFPPYTFENWEVESSVEGQLDEKNTIPHTFLHLVRRKG
                     K"
     CDS             2340..2762
                     /gene="degV"
                     /codon_start=1
                     /product="DegV family"
                     /translation="MAKQIIVTDSTSDLSHEYLKQHNIHVIPLSLTIDGKSYTDQADI
                     SSSEYIDHIENDADVKTSQPPIGRFIETYEQLAQDDVEIISIHLSSGLSGTYNTAVQA
                     SHMVDGNITVIDSKSISFGLGYQIKQIVELVLLQSKKI"
     repeat_region   complement(2735..2754)
                     /gene="IRR_IS257R1"
     CDS             complement(2794..3468)
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS257R1 transposase"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRGRGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDI
                     WLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMVKVIKAFKLKPDCHCTSK
                     YLNNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEI
                     SIMLAS"
     repeat_region   complement(3505..3524)
                     /gene="IRL_IS257R1"
     misc_feature    complement(3720..3928)
                     /gene="truncated rep"
                     /product="Truncated replication protein Rep (pseudogene)"
     repeat_region   complement(3929..3955)
                     /gene="IRR_IS257R2"
     CDS             complement(3987..4661)
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS257R2 transposase"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRGRGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDI
                     WLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCHCTSK
                     YLNNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEI
                     SIMLAS"
     repeat_region   complement(4691..4717)
                     /gene="IRL_IS257R2"
ORIGIN      
        1 ggttctgttg caaagtaaaa aatatagcta accactaatt tatcatgtca gtgttcgctt
       61 aacttgctag catgatgcta atttcgtggc atggcgaaaa tccgtagatc tgaagagacc
      121 tgcggttctt tttatataga gcgtaaatac attcaatacc ttttaaagta ttctttgctg
      181 tattgatact ttgatacctt gtctttctta ctttaatatg acggtgatct tgctcaatga
      241 ggttattcag atatttcgat gtacaatggc agtcaggttt aagtttaaaa gctttaatta
      301 ctttagccat tgctaccttc gttgaaggtg cctgatctgt aattaccttt tgaggtttac
      361 caaattgttt aatgagacgt ttaataaacg catatgctga atgattatct cgttgcttac
      421 gcaaccaaat atctaatgta tgtccctctg catcaatggc acgatataaa tagctccatt
      481 ttccttttat tttgatgtac gtctcatcaa tacgccattt gtaataagct tttttatgct
      541 ttttcttcca aatttgatat aaaattgggg catattcttg aacccaacgg tagaccgttg
      601 aatgatgaac gtttacacca cgtcccctta atatttcaga tatatcacga taactcaatg
      661 catatcttag atagtagcca acggctacag tgataacatc cttgttaaat tgtttatatc
      721 tgaaatagtt catacagaag actccttttt gttaaaatta tactataaat tcaactttgc
      781 aacagaacct cttttctagt ataatgttta ttaatatagt acatattttt ataaggagtt
      841 gtacgcatgt ataatccatt cgacgaagct tatcatggct tatgcgaaga gatattagaa
      901 atagggaata gacgcgatga tcgcacacat accgggacaa tttccaaatt tgggcatcaa
      961 ttgcgtttcg atttaaccaa agggtttcct ttacttacaa ccaaaaaggt ttcttttaaa
     1021 ttagtagcaa ctgaattatt atggtttatt aaaggagata caaacattca atacctactt
     1081 aaatataata acaatatatg gaatgaatgg gcatttgaaa attatgttca gtctgatgat
     1141 tatcatggac cagacatgac agattttgga catcgttccc agcaagaccc tgaatttaac
     1201 gagcagtata aagaagaaat gaaaaaattt aaagaacgta ttctaaacga tgatgcattt
     1261 gctaaaaaat atggcaactt agggaatgtt tatggtaaac aatggagaga ttgggaagat
     1321 aaaaatggaa atcattacga tcaattaaaa tcagttatac aacaaattaa aaccaatcca
     1381 aactctcgac gtcacattgt ttcagcatgg aatcctactg aaatcgattc aatggcatta
     1441 ccaccttgcc acacaatgtt tcaattttac gtacaagagg gtaaattaaa ttgtcaatta
     1501 tatcaaagaa gtgctgatat atttttaggt gttcctttca atattgcaag ttatgcttta
     1561 ctgacacatc ttgttgcaaa agaatgtgga cttgaggtgg gtgaattcat tcatacattt
     1621 ggagatgcac atatttattc caatcatatg gatgcaatac atacacaatt atcaagagat
     1681 agttatctac cacctcaatt aaaaattaat acagataaat caatttttga tattaattat
     1741 gaagatttag aattgataaa ttatgaatca caccctgcta ttaaagcacc tattgcggta
     1801 taaaaagatg cattaatatt tcgcatacag aaaggaggta taccatgaca ttatcaataa
     1861 ttgtcgctca cgataaacaa agagtcattg ggtaccaaaa tcaattacct tggcacttac
     1921 caaatgattt aaagcatatt aaacaactga ccactgggaa tacacttgta atggcacgga
     1981 aaacttttaa ttctataggg aagccattgc caaatagacg taacgtcgta ctcactaacc
     2041 aagcttcatt tcaccatgaa ggggtagatg ttataaactc tcttgatgaa attaaagagt
     2101 tatctggtca tgtttttata tttggaggac aaacgttata cgaagcaatg attgaccagg
     2161 tagatgatat gtatatcaca gtaatagatg gaaagtttca aggagacaca ttctttccac
     2221 catacacatt cgaaaactgg gaagtcgaat cttcagtaga aggtcaacta gatgaaaaaa
     2281 atactatacc gcatacattc ttacatttag tgcgtagaaa agggaaatag gaggcaatta
     2341 tggctaaaca aattatcgtc acggattcaa cctctgattt atcacatgaa tatttaaaac
     2401 aacataacat tcatgttata ccattaagcc tgacaatcga cgggaaatct tacactgatc
     2461 aagctgatat ctcttcaagt gagtatatcg atcatattga aaatgatgca gacgtcaaaa
     2521 caagtcaacc acctattggt cgatttattg aaacatatga gcaattagct caagatgacg
     2581 ttgaaattat aagtattcat ctttcgtcag gcttaagtgg tacttataat actgctgttc
     2641 aagcgagcca tatggtagat ggtaatatta cagtgattga ttctaaatct atttcgtttg
     2701 ggttaggtta tcaaattaag caaattgttg aattggttct gttgcaaagt aaaaaaatat
     2761 agctaaccac taatttatca tgtcagtgtt cgcttaactt gctagcatga tgctaatttc
     2821 gtggcatggc gaaaatccgt agatctgaag agacctgcgg ttctttttat atagagcgta
     2881 aatacactca atacctttta aagtattctt tgctgtattg atactttgat accttgtctt
     2941 tcttacttta atatgacggt gatcttgctc aatgaggtta ttcagatatt tcgatgtaca
     3001 atggcagtca ggtttaagtt taaaagcttt aattacttta accattgcta ccttcgttga
     3061 aggtgcctga tctgtaatta ccttttgagg tttaccaaat tgtttaatga gacgtttgat
     3121 aaacgcatat gctgaatgat tatctcgttg cttacgcaac caaatatcta atgtatgtcc
     3181 ctctgcatca atggcacgat ataaatagct ccattttcct tttattttga tgtacgtctc
     3241 atcaatacgc catttgtaat aagctttttt atgctttttc ttccaaattt gatataaaat
     3301 tggggcatat tcttgaaccc aacggtagac cgttgaatga tgaacgttta caccacgtcc
     3361 ccttaatatt tcagatatat cacgataact caatgcatat cttagatagt agccaacggc
     3421 tacagtgata acatccttgt taaattgttt atatctgaaa tagttcatac agaagactcc
     3481 tttttgttaa aattatacta taaattcaac tttgcaacag aacccgattt catttgttgc
     3541 aagttttatc gtatcagtta tagctggtat agttgcgaac tatatttcag acaaactgaa
     3601 tagataaaat taaaaccccc acaaagcttt ggcaggctgt gggggttttt tgtatatgct
     3661 agttcgcaat ccatttgtta ctttaagtat atctttcata ctatcttttg taaacttttc
     3721 tattcttccc aatcaacttg aagttgtttt aaaaatgctt ctcgttcttt ttctaattgc
     3781 ggatcatact cattttgtgt tggtttttca tatgtgcgtt cttccaacca ttttggcgtt
     3841 ttttcacgac ttatgtattg tcgtgactta cctatattcg ccatttgtgg ttgtcgttta
     3901 ttgtgaatgc gtttctcagc gtcaaaaggg ttctgttgca aagtaaaaaa tatagctaac
     3961 cactaattta tcatgtcagt gttcgcttaa cttgctagca tgatgctaat ttcgtggcat
     4021 ggcgaaaatc cgtagatctg aagagacctg cggttctttt tatatagagc gtaaatacat
     4081 tcaatacctt ttaaagtatt ctttgctgta ttgatacttt gataccttgt ctttcttact
     4141 ttaatatgac ggtgatcttg ctcaatgagg ttattcagat atttcgatgt acaatggcag
     4201 tcaggtttaa gtttaaaagc tttaattact ttagccattg ctaccttcgt tgaaggtgcc
     4261 tgatctgtaa ttaccttttg aggtttacca aattgtttaa tgagacgttt aataaacgca
     4321 tatgctgaat gattatctcg ttgcttacgc aaccaaatat ctaatgtatg tccctctgca
     4381 tcaatggcac gatataaata gctccatttt ccttttattt tgatgtacgt ctcatcaata
     4441 cgccatttgt aataagcttt tttatgcttt ttcttccaaa tttgatataa aattggggca
     4501 tattcttgaa cccaacggta gaccgttgaa tgatgaacgt ttacaccacg tccccttaat
     4561 atttcagata tatcacgata actcaatgca tatcttagat agtagccaac ggctacagtg
     4621 ataacatcct tgttaaattg tttatatctg aaatagttca tacagaagac tcctttttgt
     4681 taaaattata ctataaattc aactttgcaa cagaacc
//
