LOCUS       Tn3                     4948 bp    DNA     linear   BCT 29-SEP-2020
DEFINITION  Escherichia coli.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Escherichia.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     repeat_region   1..38
                     /gene="IRL_Tn3"
     CDS             complement(34..3039)
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 transposase"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSR
                     GDHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTR
                     REHAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILP
                     GATTLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGP
                     VTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQK
                     RMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALA
                     SACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRV
                     RRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVIN
                     KEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRS
                     LGHPTDPQEAIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPD
                     SLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMA
                     EACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGG
                     GEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDS
                     IFVLEGLLEQETGLNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDH
                     DADYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLT
                     QAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYT
                     DGQEDQLGALGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINML
                     GHYSFTLAELVTKGHLRPLKEASEAENVA"
     misc_recomb     3082..3195
                     /gene="res"
     CDS             3201..3758
                     /gene="tnpR"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 resolvase"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     CDS             3941..4801
                     /gene="blaTEM-1"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase TEM-1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     repeat_region   4911..4948
                     /gene="IRR_Tn3"
ORIGIN      
        1 ggggtctgac gctcagtgga acgaaaactc acgttaagca acgttttctg cctctgacgc
       61 ctcttttaat ggtctcagat gacctttggt caccagttct gccagcgtga aggaataatg
      121 gccgagcata ttgatatgtc cgtggcaaag cggggagagg cgtgcgatat cttcatcatt
      181 cagtgtttca ccctgcgccc ggagatgatc cagagctgcc tgcatataaa tagtgttcca
      241 taacacgacg gcgttagtga ccagccccag agctcccaac tgatcttcct gaccgtcggt
      301 atatcgtttt cttatctcac ctttttgacc gtgacagatg gctctggcaa ctgcatgacg
      361 gctttctccc cgattaagct gggtcagaat gcgccggcgg taatcttcat catcaatata
      421 attaagcaga tacagcgttt tgttgatgcg ccccacttca atgattgcct gagtcagtcc
      481 ggagggacgt tcacttttca gcaatgaacg gaccagcact gaggcctgta ctttgcccag
      541 cttcagggag cctgcggtcc ggatcatttc gtcccactga aggactattt ttcggggatc
      601 tgattgccct ctggcaatat cattcagcac gccatagtcg gcatcatggt ccattcgcca
      661 gaaaaccgaa gcaccggcat cagccaggcg tggagaaaac tggtatccca gcagccagaa
      721 aaggccaaag acaagatcgc tggcacctgc cgtatcggtc ataatttcgg ttggattcag
      781 cccggtctcc tgttccagaa ggccttccag cacaaagata gagtccctca gcgtccccgg
      841 tataacgatg ccatgaaagc cggaatactg atcggacaca aagttgtacc aggtgatccc
      901 tctgttatta ccaaagtatt tgcggttcgg tccggcattg attgttctga ctggcgtaac
      961 aaagcgcatt ccatctgcag atgccacttc tcctccaccc catatctgtg ccagtggcag
     1021 cgttgcctga aaatcaacca gtctggcatt agcgctggtg atagtttcag cccgcagata
     1081 gttcgctttt gtccagttca gccggtgtcg ggtcagtgca ggaacatttg atctgatcag
     1141 tggttccaga ccgatattgc aggcttcagc catcagcacg gcgctgatgc tgacgggcag
     1201 atcatcaact ctggcactgg cttcactggc atggaaaaac tcatcagcaa atccggtatg
     1261 ggcgttaatt tcgagcagca actccgttaa atccaccgga gggagcagat cactgatcat
     1321 tttgctcagt cgtttcagac tgtccggctc atcaagactg gcgaggggag aaattgtcaa
     1381 ccggggcttc gggccagaaa catcgagttc gacagcctca ttttcgccaa gacgtgcagc
     1441 aacctgtctg taacgactat caagctgatg gcccagagat tttattgctt cctgcgggtc
     1501 tgtcgggtgc cccaaagaac gataaacctt aatccgattt gcctgccagt cagcaccctg
     1561 tagtaatctt gcacgaggat ctccccaccg gttactgccg gtaacgtaga catccctccg
     1621 tctcagacta tcctgcagtt tactgagaaa gcagagcgtg tatccccttc gggtgatatg
     1681 tttttccttg ttaatcacca gccgtttcca tgactgactg atgatttccg ttggtgcgtc
     1741 gtcaaaaaac tgccgccgtg agctgaactc ccggctgagg tagtcacagg cattcagagt
     1801 ggtaaccccg gcaggtgcgg atgaaaattt aacggtattc agcagatggg gcaggaaacg
     1861 acgaacgcgc ccgtactgct ccaccatttc gtcatgaaaa ttatcgtctg agggccgggc
     1921 aatttcacgg acaagcgtga tgatttcagc cagcttttgc ctagggatgt agctgaacac
     1981 ctcagcacga atcgattcgt ccggtgtttc ttctttcagc aagtacgaac atgcgctggc
     2041 gagcgccaat gcagatttat ccagatcctt cagcgagcgg agccgttttt tctgcccaat
     2101 ctttctggcg tcacggatga taacggccag catggcgtcc agaacgtcca gtgcatcatc
     2161 cagcgccagc gtttcccatg caaggacaaa ggcaaccaga accgccatcc ttttctgcgg
     2221 tgacatcctg gcaatattga acaccgaagt cataccagca taacgtgcga gatttttcag
     2281 gcgcacagcc gggagtgtac tcaggttttc agcatgcagg ccaaaatcgt tcagagtttt
     2341 ccagcgttca attgcttcat taaacgccgg accactgatg gtcacagggc cttttttcag
     2401 tgattccagt aaagacaggc ggctgcaatc agttggcccc agcagcatct ccagctgtga
     2461 acgctgttcg gctgacggta tcagtgccag tttgttccac aggcgcaacg tcgccttttc
     2521 ccttacctct gaaatcaacc gggtcagcgt ggtggctccg gggagaataa tacgatgttg
     2581 cataagccac cctgtcgcca ggtcgaaaag caggccagga cgttcgttgc ttatccagct
     2641 ccgggtatat aaaagacggg taaggcgaaa tgtccagggc caggcaaatt cacgatactg
     2701 atagtgctga cgtatcagcg ctgcatgctc acggcgggta ttttccctct gaccgtattc
     2761 tgcaagaacg gtgatatcac gaatcccgag ctgtctggcg gtaaaatgcc ggacgccgga
     2821 aggaatatga ttcatgtcag taagaaaagt gcccagaaaa cgcacacacc cgatttgcag
     2881 ggcaatacca agtcgattgt gatcaccccg gcttttcccg ataaattctt tatccgcttc
     2941 atcaagatga aaataacgcg ccagctgaag ttcatcgggt tcgccagtga acctgccata
     3001 actctcaacc tgctcagtgg tcaaaaaatc aacgggcata tcggcctccc tgcctgacgg
     3061 cttttttaac acaactgcaa ccgttcgaaa tattataaat tatcagacat agtaaaacgg
     3121 cttcgtttga gtgtccatta aatcgtcatt ttggcataat agacacatcg tgtctgatat
     3181 tcgatttaag gtacattttt atgcgaattt ttggttatgc gcgggtctca accagccagc
     3241 agtccctcga tattcagatc agagcgctca aagatgcagg ggtaaaagct aaccgcatct
     3301 ttaccgacaa ggcatccggc agttcaacag atcgggaagg gctggatttg ctgaggatga
     3361 aggtggagga aggtgatgtc attctggtga agaagctcga ccgtcttggc cgcgacaccg
     3421 ccgacatgat ccaactgata aaagagtttg atgctcaggg tgtagcggtt cggtttattg
     3481 acgacgggat cagtaccgac ggtgatatgg ggcaaatggt ggtcaccatc ctgtcggctg
     3541 tggcacaggc tgaacgccgg aggatcctag agcgcacgaa tgagggccga caggaagcaa
     3601 agctgaaagg aatcaaattt ggccgcaggc gtaccgtgga caggaacgtc gtgctgacgc
     3661 ttcatcagaa gggcactggt gcaacggaaa ttgctcatca gctcagtatt gcccgctcca
     3721 cggtttataa aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3781 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3841 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3901 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3961 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4021 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4081 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4141 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     4201 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4261 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4321 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4381 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4441 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     4501 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4561 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4621 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4681 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4741 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4801 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4861 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4921 gttttcgttc cactgagcgt cagacccc
//
