LOCUS       Tn1696                 16318 bp    DNA     linear   BCT 29-SEP-2020
DEFINITION  Escherichia coli.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Escherichia.
REFERENCE   1  (bases 1 to 16318)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn1696
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16318)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..16318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     repeat_region   1..38
                     /gene="IRL_Tn1696"
     CDS             complement(34..3006)
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn1696 transposase"
                     /translation="MPRRSILSATERESLLALPDAKDELIRHYTFNETDLSVIRQRRG
                     AANRLGFAVQLCYLRFPGTFLGVDEPPFPPLLRMVAAQLKMPVESWSEYGQREQTRRE
                     HLVELQTVFGFKPFTMSHYRQAVHTLTELALQTDKGIVLASALVENLRRQSIILPAMN
                     AIERASAEAITRANRRIYAALTDSLLSPHRQRLDELLKRKDGSKVTWLAWLRQSPAKP
                     NSRHMLEHIERLKSWQALDLPAGIERQVHQNRLLKIAREGGQMTPADLAKFEVQRRYA
                     TLVALAIEGMATVTDEIIDLHDRIIGKLFNAAKNKHQQQFQASGKAINDKVRMYGRIG
                     QALIEAKQSGSDPFAAIEAVMPWDTFAASVTEAQTLARPADFDFLHHIGESYATLRRY
                     APQFLGVLKLRAAPAAKGVLDAIDMLRGMNSDSARKVPADAPTAFIKPRWAKLVLTDD
                     GIDRRYYELCALSELKNALRSGDVWVQGSRQFKDFDEYLVPVEKFATLKLASELPLAV
                     ATDCDQYLHDRLELLEAQLATVNRMAAANDLPDAIITTASGLKITPLDAAVPDAAQAM
                     IDQTAMLLPHLKITELLMEVDEWTGFTRHFTHLKTSDTAKDKTLLLTTILADAINLGL
                     TKMAESCPGTTYAKLSWLQAWHIRDETYSTALAELVNAQFRQPFAGNWGDGTTSSSDG
                     QNFRTGSKAESTGHINPKYGSSPGRTFYTHISDQYAPFSAKVVNVGIRDSTYVLDGLL
                     YHESDLRIEEHYTDTAGFTDHVFGLMHLLGFRFAPRIRDLGETKLFIPKGDAAYDALK
                     PMISSDRLNIKQIRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELG
                     RIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFYRLGEIRDRSFEQQRYRA
                     SGLNLVTAAIVLWNTVYLERATSALRGNGTALDDTLLQYLSPLGWEHINLTGDYLWRS
                     SAKVGAGKFRPLRPLPPA"
     CDS             complement(3009..3566)
                     /gene="tnpR"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn1696 resolvase"
                     /translation="MQGQRIGYVRVSSFDQNPERQLEGVQVARVFTDKASGKDTQRPE
                     LERLLAFVREGDTVVVHSMDRLARNLDDLRRIVQGLTQRGVRMEFVKEGLKFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIVLAKQRGAYRGRKKSLNSEQIAELKRRVAA
                     GDQKTLVARDFGISRETLYQYLRED"
     misc_recomb     3587..3669
                     /gene="truncated res-5'"
     repeat_region   3665..3669
                     /gene="DR_In4"
     repeat_region   3670..3694
                     /gene="IRi_In4"
     CDS             complement(3872..4885)
                     /gene="intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="Integrase/recombinase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVNWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPTLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     misc_recomb     4966..5049
                     /gene="attI1"
     CDS             4987..5520
                     /gene="aacC1"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside-(3)-acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAAL
                     DLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQP
                     RSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKL
                     GIREEVMHFDIDPSTAT"
     misc_recomb     5515..5623
                     /gene="attC_aacC1"
     CDS             5638..5883
                     /gene="gcuE"
                     /codon_start=1
                     /transl_table=11
                     /product="gcuE protein"
                     /translation="MRAATYFSAPPCSKGFLAFWASSACAASATSYPSCSAAPLSWSS
                     GFSWAAPFFKAGRSLLAFGSNSAVKRTRILRAAYLGR"
     CDS             5901..6680
                     /gene="aadA2"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside adenyltransferase"
                     /translation="MTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKPYSDI
                     DLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYPAKRE
                     LQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQDLFE
                     ALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPAQYQP
                     VLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
     misc_recomb     5915..5975
                     /gene="attC_gcuE"
     misc_recomb     6682..6741
                     /gene="attC_aadA2"
     CDS             6942..8201
                     /gene="cmlA1"
                     /codon_start=1
                     /transl_table=11
                     /product="Chloramphenicol transporter"
                     /translation="MSSKNFSWRYSLAATVLLLSPFDLLASLGMDMYLPAVPFMPNAL
                     GTTASTIQLTLTTYLVMIGAGQLLFGPLSDRLGRRPVLLGGGLAYVVASMGLALTSSA
                     EVFLGLRILQACGASACLVSTFATVRDIYAGREESNVIYGILGSMLAMVPAVGPLLGA
                     LVDMWLGWRAIFAFLGLGMIAASAAAWRFWPETRVQRVAGLQWSQLLLPVKCLNFWLY
                     TLCYAAGMGSFFVFFSIAPGLMMGRQGVSQLGFSLLFATVAIAMVFTARFMGRVIPKW
                     GSPSVLRMGMGCLIAGAVLLAITEIWALQSVLGFIAPMWLVGIGVATAVSVAPNGALR
                     GFDHVAGTVTAVYFCLGGVLLGSIGTLIISLLPRNTAWPVVVYCLTLATVVLGLSCVS
                     RVKGSRGQGEHDVVALQSAESTSNPNR"
     misc_recomb     8221..8290
                     /gene="attC_cmlA1"
     CDS             8393..8740
                     /gene="qacED1"
                     /codon_start=1
                     /transl_table=11
                     /product="Quaternary ammonium compound resistance protein"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     CDS             8734..9573
                     /gene="sul1"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydropteroate synthase"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     CDS             9701..10201
                     /gene="orf5"
                     /codon_start=1
                     /transl_table=11
                     /product="Putative acetyltransferase"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     CDS             10225..10512
                     /gene="orf6"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MSGWDGPVASRSLAVRPRCLGCARGCRYPPLPSSTLDNAAAVIA
                     ADARAGLFGPVRLARPSACLGEISGGRINGGASPPFVRVKAEDSSLKNIRL"
     repeat_region   complement(10524..10548)
                     /gene="IRt_In4"
     repeat_region   10647..10660
                     /gene="IRL_IS6100"
     CDS             10708..11472
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6100 transposase"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     repeat_region   11513..11526
                     /gene="IRR_IS6100"
     misc_feature    11527..11793
                     /gene="truncated tnpA"
                     /product="Truncated IS6100 transposase (pseudogene)"
     repeat_region   11834..11847
                     /gene="IRR_IS6100"
     repeat_region   11975..11999
                     /gene="IRt_In4"
     repeat_region   12000..12004
                     /gene="DR_In4"
     misc_recomb     12005..12041
                     /gene="res-3'"
     CDS             complement(12031..12243)
                     /gene="urf2Y"
                     /codon_start=1
                     /transl_table=11
                     /product="Urf2Y protein"
                     /translation="MNANAPNTASCTTCCECCKEIPLDAAFTPEGAEYVEHFCGLDCY
                     ERFQARAKAATESDIAPVPGGSQPSD"
     CDS             complement(12309..12545)
                     /gene="merE"
                     /codon_start=1
                     /transl_table=11
                     /product="Mercuric transport protein MerE"
                     /translation="MNSPEHLPSETHKPITGYLWGALAVLTCPCHLPILAIVLAGTTA
                     GAFIGEHWGIAALTLTGLFVLSVTRLLRAFKGRS"
     CDS             complement(12542..12907)
                     /gene="merD"
                     /codon_start=1
                     /transl_table=11
                     /product="Mercuric resistance transcriptional regulator
                     MerD"
                     /translation="MSAYTVSRLALDAGVSVHIVRDYLLRGLLRPVAYTTGGYGLFDD
                     TALQRLRFVRAAFEAGIGLDALARLCRALDAADGDGASAQLAVLRQLVERRREALASL
                     EMQLAAMPTEPAQHAESLP"
     CDS             complement(12925..14610)
                     /gene="merA"
                     /codon_start=1
                     /transl_table=11
                     /product="Mercuric ion reductase MerA"
                     /translation="MTTLKITGMTCDSCAAHVKEALEKVPGVQSALVSYPKGTAQLAI
                     EAGTSSDALTTAVAGLGYEATLADAPPTDNRAGLLDKMRGWIGAADKPSGNERPLQVV
                     VIGSGGAAMAAALKAVEQGAQVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRES
                     PFDGGMPPTPPTILRERLLAQQQARVEELRHAKYEGILDGNSAITVLHGEARFKDDQS
                     LIVSLNEGGERVVMFDRCLVATGASPAVPPIPGLKESPYWTSTEALASDTIPERLAVI
                     GSSVVALELAQAFARLGSKVTALARNTLFFREDPAIGEAVTAAFRAEGIEVLEHTQAS
                     QVAHMDGEFVLTTTHGELRADKLLVATGRTPNTRSLALEAAGVAVNAQGAIVIDKGMR
                     TSSPNIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALDLTAMPAVVFTDPQVATV
                     GYSEAEAHHDGIETDSRLLTLDNVPRALANFDTRGFIKLVIEEGSGRLIGVQAVAPEA
                     GELIQTAVLAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFSKDVKQLSCCAG"
     CDS             complement(14649..15074)
                     /gene="merC"
                     /codon_start=1
                     /transl_table=11
                     /product="Mercuric transport protein MerC"
                     /translation="MGLITRIAGKTGALGSVVSAMGCAACFPAIASFGAAIGLGFLSQ
                     YEGLFIGILLPMFAGIALLANAIAWLNHRQWRRTALGTIGPILVLAAVFLMRAYGWQS
                     GGLLYVGLALMVGVSVWDFISPAHRRCGPDSCELPEQRG"
     CDS             complement(15102..15377)
                     /gene="merP"
                     /codon_start=1
                     /transl_table=11
                     /product="Mercuric transport protein periplasmic component
                     MerP"
                     /translation="MKKLFAALALAAVVAPVWAATQTVTLSVPGMTCASCPITVKHAL
                     SKVEGVSKTDVSFDKRQAVVTFDDAKTNVQKLTKATEDAGYPSSLKR"
     CDS             complement(15393..15758)
                     /gene="merT"
                     /codon_start=1
                     /transl_table=11
                     /product="Mercuric transport protein MerT"
                     /translation="MSEPQKSEPQNGRGALFAGGLAAILASACCLGPLVLIALGFSGA
                     WIGNLTVLEPYRPIFIGAALVALFFAWRRIYRPAQACKPGEVCAIPQVRATYKLIFWI
                     VAALVLVSLGFPYVMPFFY"
     CDS             15830..16285
                     /gene="merR"
                     /codon_start=1
                     /transl_table=11
                     /product="Mercuric resistance transcriptional regulator
                     MerR"
                     /translation="MQINFENLTIGVFAKAAGVNVETIRFYQRKGLLPEPDKPYGSIR
                     RYGEADVTRVRFVKSAQRLGFSLDEIAELLRLEDGTHCEEASGLAEHKLKDVREKMAD
                     LARMEAVLSELVCACHARKGNVSCPLIASLQDGTKLAASARGSHGVTTP"
     repeat_region   16281..16318
                     /gene="IRR_Tn1696"
ORIGIN      
        1 ggggtcgtct cagaaaacgg aaaataaagc acgctaagcc ggtggcagcg gtcgcaatgg
       61 cctaaacttc cccgcaccga ccttggcgct gctgcgccat aggtaatcgc cggtcaggtt
      121 gatgtgctcc caccccagcg gcgacagata ttgcaacaat gtgtcgtcca gcgccgtgcc
      181 gttgccacgc aaagcactgg tggcacgctc cagatatacc gtgttccaca acacgatggc
      241 cgccgtcacc agattgaggc cgctggcccg gtagcgctgc tgctcaaaac tgcggtcgcg
      301 gatttcaccc aatcggtaga agaagaccgc cctggccagc gcgttgcgcg cctcgccctt
      361 attcagcccc gcatggacgc ggcggcgcag ctccacgctt tgcagccaat ccaaaatgaa
      421 cagcgtgcgc tcgatgcgcc ccagctcgcg caacgccacg gccaagccgt tctggcgcgg
      481 gtagctgccg agtttgcgca gcatcagcga agccgttacc gtgccttgct tgatggaggt
      541 ggccagccgc agaatttcat cccaatgggc gcgtatttgc ttgatgttca gcctgtcgct
      601 gctaatcatc ggcttgagcg cgtcataggc ggcatcgccc ttggggatga atagcttggt
      661 ttcgcccaag tcacggatac gcggcgcgaa gcgaaatccc agcaaatgca tcaagccaaa
      721 cacgtgatcg gtgaagcctg ccgtgtcggt gtagtgttcc tcgatgcgca agtccgactc
      781 gtggtacagc aggccatcaa gcacgtaagt tgaatcacga atgcccacgt tgaccacctt
      841 ggcactgaag ggcgcgtact ggtcggagat atgggtgtag aaagtccgtc ctggactgct
      901 tccatacttc gggttgatat gaccagtgct ttctgctttg ctgccggttc tgaagttctg
      961 gccgtccgac gatgacgtgg tgccgtcacc ccagttgccg gcgaagggtt gccgaaactg
     1021 cgcattcacc agctcggcca gcgccgtcga ataggtttca tcgcggatgt gccaggcttg
     1081 cagccaagac agcttggcgt aggtggtgcc agggcaggac tcggccattt tggtcagacc
     1141 caggttgatc gcgtcggcca ggatcgtcgt caacagcaag gttttgtcct tggccgtgtc
     1201 gctggtcttc aggtgtgtga agtggcgggt gaagcccgtc cattcatcga cctccatcag
     1261 caactcggtg attttgaggt gcggcagcag catagctgtc tggtcgatca tggcttgcgc
     1321 ggcgtctggt actgccgcgt ccagcggcgt gatcttcagg cctgacgcgg tggtgatgat
     1381 ggcatccggt aagtcgttgg ccgcagccat gcggttgact gtggcgagtt gcgcctccaa
     1441 caattccaac cggtcatgca ggtattggtc gcagtcggtg gccactgcca gcggcaattc
     1501 gctggccagc ttcaaagtgg cgaacttctc gaccggcacc aggtattcgt cgaagtcctt
     1561 gaactggcga gaaccctgca cccagacatc accggagcgc agcgcgttct tcagctccga
     1621 cagggcgcat aactcgtagt aacgccggtc gatgccgtcg tcggtcagaa ccagctttgc
     1681 ccagcgcggc ttgatgaatg cggttggcgc atcggcgggc accttgcgcg cgctgtcgct
     1741 gttcatgccg cgcagcatgt cgatggcatc gagcacaccc ttggcggcgg gcgcagcccg
     1801 caatttgagc acgcccagga actgcggcgc gtagcggcgt agcgtggcat agctttcacc
     1861 gatgtggtgc aggaaatcaa agtcggcagg ccgcgccaat gtttgcgctt cggtgacgct
     1921 ggcggcgaag gtgtcccagg gcataacggc ctcgatggcg gcgaacggat cgctgccgct
     1981 ttgcttggcc tcaatcaacg cttgaccgat gcgcccatac atccgcacct tgtcgttgat
     2041 cgccttgccg gaagcctgga actgctgctg atgcttgttc ttggccgcgt tgaacagctt
     2101 gccgatgatg cgatcgtgaa ggtcgatgat ttcatcggtg acggtggcca tgccttcgat
     2161 ggccagcgct accagcgtgg catagcgtcg ttgcacctcg aactttgcca gatcagcagg
     2221 cgtcatctgg ccaccttcac gagcgatttt gagcaggcgg ttctggtgaa cctgccgctc
     2281 gatgcctgcg ggcagatcaa gtgcttgcca ggatttcagg cgctcaatat gttcgagcat
     2341 gtggcgagag ttcggtttgg caggcgactg gcgcagccat gccagccacg tcactttact
     2401 gccgtccttg cgcttgagaa gttcgtccag gcgctgacgg tggggtgata acaaagaatc
     2461 ggtcagcgcc gcgtaaatgc gtcggttggc acgggtgatg gcctcggcgc ttgcgcgctc
     2521 gatggcattc atggcgggca ggataatgct ctgccgccgc agattctcga caagtgcgct
     2581 cgccagcacg atgcctttgt cggtctgcaa ggccagctcg gtcaatgtat gcacggcttg
     2641 ccgatagtgg ctcatggtga agggcttgaa cccaaaaacc gtttgcagct cgaccaagtg
     2701 ctcccgccgt gtctgttcgc gctggccgta ctcgctccaa ctttccactg gcatcttgag
     2761 ttgcgcggcc accatgcgca acaggggcgg aaacggaggc tcatcgacgc ccaaaaaggt
     2821 gccagggaat cgcaagtagc aaagctgcac agcgaagccc aatcgattcg cggcgccgcg
     2881 acgctgacgg atcaccgaca ggtcggtttc gttgaacgtg tagtgccgta tcagttcgtc
     2941 tttggcatct ggcagtgcca gcaggctttc gcgctcggtg gcggacagga ttgagcggcg
     3001 tggcatggtc agtcttcccg caggtactgg tacaaggttt cgcggctgat gccgaagtca
     3061 cgggccacca aggttttttg gtcgcctgcc gcaactcgcc gtttcaactc ggcaatttgt
     3121 tcgctgttca gcgatttctt tcgtccccgg taggcaccgc gctgcttggc cagcacgatt
     3181 ccctcgcgct gacgttcgcg gatcagggcg cgctcgaact cagcgaaggc tcccatgacc
     3241 gacagcatca gattggccat cggtgagtcc tcgccggtga acttcagccc ttctttgacg
     3301 aactccatgc gcacgccccg ttgtgtcagc ccttggacga tgcggcgcag gtcatcaagg
     3361 ttgcgtgcca gcctgtccat gctatgcacc accacggtgt cgccctcgcg gacgaaggcc
     3421 agcagccttt ccagctcggg acgctgggtg tccttgccag aagccttgtc ggtgaacacc
     3481 cgcgccacct gaacaccctc caattgccgt tccgggttct ggtcgaagct gctgacgcgg
     3541 acatagccga tgcgttgacc ttgcaagatg cctccaaagg caaaagtgtc aggatgaaat
     3601 ctattacctt tgacggaata tgtcaatcaa taggaaattt aactctattc tgacatcgtt
     3661 tgcacatggt gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat
     3721 agcagcggag gggttggatc catcaggcaa cgacgggctg ctgccggcca tcagcggacg
     3781 cagggaggac tttccgcaac cggccgttcg atgcggcacc gatggccttc gcgcaggggt
     3841 agtgaatccg ccaggattga cttgcgctgc cctacctctc actagtgagg ggcggcagcg
     3901 catcaagcgg tgagcgcact ccggcaccgc caactttcag cacatgcgtg taaatcatcg
     3961 tcgtagagac gtcggaatgg ccgagcagat cctgcacggt tcgaatgtcg taaccgctgc
     4021 ggagcaaggc cgtcgcgaac gagtggcgga gggtgtgcgg tgtggcgggc ttcgtgatgc
     4081 ctgcttgttc tacggcacgt ttgaaggcgc gctgaaaggt ctggtcatac atgtgatggc
     4141 gacgcacgac accgctccgt ggatcggtcg aatgcgtgtg ctgcgcaaaa acccagaacc
     4201 acggccagga atgcccggcg cgcggatact tccgctcaag ggcgtcggga agcgcaacgc
     4261 cgctgcggcc ctcggcctgg tccttcagcc accatgcccg tgcacgcgac agctgctcgc
     4321 gcagcgtggg tgccaagctc tcgggtaaca tcaaggcccg atccttggag cccttgccct
     4381 cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct
     4441 cactgatccg catgcccgtt ccatacagaa gctgggcgaa caaacgatgc tcgccttcca
     4501 gaaaaccgag gatgcgaacc acttcatccg gggtcagcac caccggcaag cgccgcgacg
     4561 gccgaggtct tccgatctcc tgaagccagg gcagatccgt gcacagcacc ttgccgtaga
     4621 agaacagcaa ggccgccaat gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg
     4681 ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac
     4741 cgtggaaacg gatgaaggca cgaacccagt tgacataagc ctgttcggtt cgtaaactgt
     4801 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     4861 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagtctatgc
     4921 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     4981 gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     5041 ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga ccaagtcaaa
     5101 tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc cacctactcc
     5161 caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac attcatcgcg
     5221 cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt tctgcccagg
     5281 tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg cgagcaccgg
     5341 aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt
     5401 gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc tctctataca
     5461 aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac cgccacctaa
     5521 caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa attgtcacaa
     5581 cgccgcggcc gcaaagcgct ccggcttaac tcaggcgtta gtcccatcac ccgctttatg
     5641 cgcgcagcca cttatttcag cgctccgccc tgttccaaag gctttctagc cttttgggcc
     5701 tccagcgctt gtgcagcaag cgccaccagc tatccttctt gcagcgctgc gccgctttcg
     5761 tggtccagcg gtttttcttg ggctgcgccc ttcttcaagg ccgggcgctc ccttttggcc
     5821 ttcgggtcta actctgcggt caagcggacc cgcattctgc gggccgctta ccttggccgt
     5881 tagacatcat gagggtagcg gtgaccatcg aaatttcgaa ccaactatca gaggtgctaa
     5941 gcgtcattga gcgccatctg gaatcaacgt tgctggccgt gcatttgtac ggctccgcag
     6001 tggatggcgg cctgaagcca tacagcgata ttgatttgtt ggttactgtg gccgtaaagc
     6061 ttgatgaaac gacgcggcga gcattgctca atgaccttat ggaggcttcg gctttccctg
     6121 gcgagagcga gacgctccgc gctatagaag tcacccttgt cgtgcatgac gacatcatcc
     6181 cgtggcgtta tccggctaag cgcgagctgc aatttggaga atggcagcgc aatgacattc
     6241 ttgcgggtat cttcgagcca gccatgatcg acattgatct agctatcctg cttacaaaag
     6301 caagagaaca tagcgttgcc ttggtaggtc cggcagcgga ggaattcttt gacccggttc
     6361 ctgaacagga tctattcgag gcgctgaggg aaaccttgaa gctatggaac tcgcagcccg
     6421 actgggccgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcaa
     6481 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaataaaa cgcctacctg
     6541 cccagtatca gcccgtctta cttgaagcta agcaagctta tctgggacaa aaagaagatc
     6601 acttggcctc acgcgcagat cacttggaag aatttattcg ctttgtgaaa ggcgagatca
     6661 tcaagtcagt tggtaaatga tgtctaacaa ttcgttcaag ccgaccgcgc tacgcgcggc
     6721 ggcttaactc cggcgttggg cgcacaataa ggctccttgc agagttgctt gaaagttgtt
     6781 acgattcaaa ttcaatcatg agatagtcag cagatgagca cttccaagaa cgcagacaag
     6841 taagccgcag caaccttcat ttttcggttg ttgcggcgtt ctcatgaatc cttttgctct
     6901 acgggagcgc cgccaaatcc tttgttcaag gagatggttt cgtgagctca aaaaacttta
     6961 gttggcggta ctcccttgcc gccacggtgt tgttgttatc accgttcgat ttattggcat
     7021 cactcggcat ggacatgtac ttgccagcag tgccgtttat gccaaacgcg cttggtacga
     7081 cagcgagcac aattcagctt acgctgacaa cgtacttggt catgattggt gccggtcagc
     7141 tcttgtttgg accgctatcg gaccgactgg ggcgccgccc cgttctactg ggaggtggcc
     7201 tcgcctacgt tgtggcgtca atgggcctcg ctcttacgtc atcggctgaa gtctttctgg
     7261 ggcttcggat tcttcaggct tgtggtgcct cggcgtgcct tgtttccaca tttgcaacag
     7321 tacgtgacat ttacgcaggt cgcgaggaaa gtaatgtcat ttacggcata ctcggatcca
     7381 tgctggccat ggtcccggcg gtaggcccat tgctcggagc gctcgtcgac atgtggcttg
     7441 ggtggcgggc tatctttgcg tttctaggtt tgggcatgat cgctgcatct gcagcagcgt
     7501 ggcgattctg gcctgaaacc cgggtgcaac gagttgcggg cttgcaatgg tcgcagctgc
     7561 tactccccgt taagtgcctg aacttctggt tgtacacgtt gtgttacgcc gctggaatgg
     7621 gtagcttctt cgtctttttc tccattgcgc ccggactaat gatgggcagg caaggtgtgt
     7681 ctcagcttgg cttcagcctg ctgttcgcca cagtggcaat tgccatggtg tttacggctc
     7741 gttttatggg gcgtgtgata cccaagtggg gcagcccaag tgtcttgcga atgggaatgg
     7801 gatgcctgat agctggagca gtattgcttg ccatcaccga aatatgggct ttgcagtccg
     7861 tgttaggctt tattgctcca atgtggctag tgggtattgg tgtcgccaca gcggtatctg
     7921 tggcgcccaa tggcgctctt cgaggattcg accatgttgc tggaacggtc acggcagtct
     7981 acttctgctt gggcggtgta ctgctaggaa gcatcggaac gttgatcatt tcgctgttgc
     8041 cgcgcaacac ggcttggccg gttgtcgtgt actgtttgac ccttgcaaca gtcgtgctcg
     8101 gtctgtcttg tgtttcccga gtgaagggct ctcgcggcca gggggagcat gatgtggtcg
     8161 cgctacaaag tgcggaaagt acatcaaatc ccaatcgttg agagaatgtg gcaagctatc
     8221 gcccaacaaa tcgctgcagc cgacccaaaa ccgctacgcg gtttcggtcg gctgagctca
     8281 ggcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     8341 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg
     8401 ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatccg cattaaaatc
     8461 tagcgagggc tttactaagc ttgccccttc cgccgttgtc ataatcggtt atggcatcgc
     8521 attttatttt ctttctctgg ttctgaaatc catccctgtc ggtgttgctt atgcagtctg
     8581 gtcgggactc ggcgtcgtca taattacagc cattgcctgg ttgcttcatg ggcaaaagct
     8641 tgatgcgtgg ggctttgtag gtatggggct cataattgct gcctttttgc tcgcccgatc
     8701 cccatcgtgg aagtcgctgc ggaggccgac gccatggtga cggtgttcgg cattctgaat
     8761 ctcaccgagg actccttctt cgatgagagc cggcggctag accccgccgg cgctgtcacc
     8821 gcggcgatcg aaatgctgcg agtcggatca gacgtcgtgg atgtcggacc ggccgccagc
     8881 catccggacg cgaggcctgt atcgccggcc gatgagatca gacgtattgc gccgctctta
     8941 gacgccctgt ccgatcagat gcaccgtgtt tcaatcgaca gcttccaacc ggaaacccag
     9001 cgctatgcgc tcaagcgcgg cgtgggctac ctgaacgata tccaaggatt tcctgaccct
     9061 gcgctctatc ccgatattgc tgaggcggac tgcaggctgg tggttatgca ctcagcgcag
     9121 cgggatggca tcgccacccg caccggtcac cttcgacccg aagacgcgct cgacgagatt
     9181 gtgcggttct tcgaggcgcg ggtttccgcc ttgcgacgga gcggggtcgc tgccgaccgg
     9241 ctcatcctcg atccggggat gggatttttc ttgagccccg caccggaaac atcgctgcac
     9301 gtgctgtcga accttcaaaa gctgaagtcg gcgttggggc ttccgctatt ggtctcggtg
     9361 tcgcggaaat ccttcttggg cgccaccgtt ggccttcctg taaaggatct gggtccagcg
     9421 agccttgcgg cggaacttca cgcgatcggc aatggcgctg actacgtccg cacccacgcg
     9481 cctggagatc tgcgaagcgc aatcaccttc tcggaaaccc tcgcgaaatt tcgcagtcgc
     9541 gacgccagag accgagggtt agatcatgcc tagcattcac cttccggccg cccgctagcg
     9601 gaccctggtc aggttccgcg aaggtgggcg cagacatgct gggctcgtca ggatcaaact
     9661 gcactatgag gcggcggttc ataccgcgcc aggggagcga atggacagcg aggagcctcc
     9721 gaacgttcgg gtcgcctgct cgggtgatat cgacgaggtt gtgcggctga tgcacgacgc
     9781 tgcggcgtgg atgtccgcca agggaacgcc cgcctgggac gtcgcgcgga tcgaccggac
     9841 attcgcggag accttcgtcc tgagatccga gctcctagtc gcgagttgca gcgacggcat
     9901 cgtcggctgt tgcaccttgt cggccgagga tcccgagttc tggcccgacg ccctcaaggg
     9961 ggaggccgca tatctgcaca agctcgcggt gcgacggaca catgcgggcc ggggtgtcag
    10021 ctccgcgctg atcgaggctt gccgccatgc cgcgcgaacg caggggtgcg ccaagctgcg
    10081 gctcgactgc cacccgaacc tgcgtggcct atacgagcgg ctcggattca cccacgtcga
    10141 cactttcaat cccggctggg atccaacctt catcgcagaa cgcctagaac tcgaaatcta
    10201 acgtccgttc gggcatcgag gtccatgtcg gggtgggacg ggcccgtggc ttcaagatca
    10261 cttgcagtcc gaccgcgatg tcttggttgc gcgagaggtt gtcgatatcc tccacttcca
    10321 tcatcaaccc tggataatgc cgccgccgtc atcgccgccg acgcccgtgc cgggcttttc
    10381 gggcctgtca ggcttgctcg gccttcagcc tgcctgggcg agatctccgg cggacggatt
    10441 aacggcggag cttcgccgcc tttcgtgcgt gtgaaggccg aagatagttc tctcaaaaac
    10501 atccgtttat gagagatacc aaatgtcatt ttcagaagac gactgcacca gttgattggg
    10561 cgtaatggct gttgtgcagc cagctcctga cagttcaata tcagaagtga tctgcaccaa
    10621 tctcgactat gctcaatact cgtgtgggct ctgttgcaaa aatcgtgaag cttgagcatg
    10681 cttggcggag attggacgga cggaacgatg acggatttca agtggcgcca tttccagggt
    10741 gatgtgatcc tgtgggcggt gcgctggtat tgtcgctatc cgatcagcta tcgcgacctt
    10801 gaggaaatgc tggcggaacg cggcatttcg gtcgaccata cgacgatcta tcgctgggtc
    10861 cagtgctacg ccccggagat ggagaagcgg ctgcgctggt tctggcggcg tggctttgat
    10921 ccgagctggc gcctggatga aacctacgtc aaggtgcggg gcaagtggac ctacctgtac
    10981 cgggcagtcg acaagcgggg cgacacgatc gatttctacc tgtcgccgac ccgcagcgcc
    11041 aaggcagcga agcggttcct gggcaaggcc ctgcgaggcc tgaagcactg ggaaaagcct
    11101 gccacgctca ataccgacaa agcgccgagc tatggtgcag cgatcaccga attgaagcgc
    11161 gaaggaaagc tggaccggga gacggcccac cggcaggtga agtatctcaa taacgtgatc
    11221 gaggccgatc acggaaagct caagatactg atcaagccgg tgcgcggttt caaatcgatc
    11281 cccacggcct atgccacgat caagggattc gaagtcatgc gagccctgcg caaaggacag
    11341 gctcgcccct ggtgcctgca gcccggcatc aggggcgagg tgcgccttgt ggagagagct
    11401 tttggcattg ggccctcggc gctgacggag gccatgggca tgctcaacca ccatttcgca
    11461 gcagccgcct gatcggcgca gagcgacagc ctacctctga ctgccgccaa tctttgcaac
    11521 agagccctca ataacgtgat cgaggccgat cacggaaagc tcaagatact gatcaagccg
    11581 gtgcgcggtt tcaaatcgat ccccacggcc tatgccacga tcaagggatt cgaagtcatg
    11641 cgagccctgc gcaaaggaca ggctcgcccc tggtgcctgc agcccggcat caggggcgag
    11701 gtgcgccttg tggagagagc ttttggcatt gggccctcgg cgctgacgga ggccatgggc
    11761 atgctcaacc accatttcgc agcagccgcc tgatcggcgc agagcgacag cctacctctg
    11821 actgccgcca atctttgcaa cagagcctcc gtcgccatgc tcacctcgct ttggtgcaca
    11881 cgagtattga gcatagtcga gattggtgca gatcacttct gatattgaac tgtcaggagc
    11941 tggctgcaca acagccatta cgcccaatca actggtgcag tcgtcttctg aaaatgacac
    12001 atggcatctg acatcaagtt agggtatgcc tcaatctgac ggctgcgaac cgccagggac
    12061 aggcgcaatg tcagattctg tcgcggcctt ggcgcgtgcc tggaagcgtt catagcaatc
    12121 cagcccgcag aaatgttcga cgtattccgc gccttccggg gtgaaggcgg catcgagcgg
    12181 aatttctttg cagcattcgc agcaggtggt gcaactggca gtgttcgggg cgtttgcgtt
    12241 catggtggta ctcctccaga ttggtagcga agctcaaagc tgcccactct cggctggctg
    12301 ggaagcggtc atgatcttcc cttgaaggcc cgcagcagcc gcgtcacaga caggacaaac
    12361 aagccggtca gcgtgagggc tgcaataccc cagtgctccc cgatgaacgc gccggccgtc
    12421 gtgccggcta gcacaatggc gagaatcggc aaatggcagg gacaggtgag cacggccagc
    12481 gcgccccaca agtagccggt gatcggtttg tgcgtctcag acggcaagtg ctctgggctg
    12541 ttcatggcag actctccgcg tgctgtgccg gttcggttgg catggcggcc agttgcattt
    12601 cgaggctggc cagggcctcg cgccgacgct cgacgagttg ccgcaacacg gcaagctgcg
    12661 cagacgcacc gtcaccgtcc gcagcatcca gcgcccggca cagccgcgcc agtgcgtcca
    12721 ggccgatacc cgcttcgaag gcagcccgta caaagcgcag ccgttgcaac gcggtgtcat
    12781 cgaacaagcc gtagccgccc gtggtgtacg cgaccggccg tagcaatccg cgcagcaggt
    12841 agtcgcgcac gatatgcacg ctcaccccgg catcaagggc cagccgggac actgtgtagg
    12901 cgctcattga acacctcctt ttcctcatcc ggcgcagcac gaaagctgct tcacgtcctt
    12961 gctgaaggtc tgcgccgcga gcttcagccc ttcgaccatg gtcaggtagg ggaacaattg
    13021 gtcggccagt tcctgcacgg tcatacggtt gcgaatggcg agcaccgccg tctggatcag
    13081 ttcacccgct tccggggcca ccgcttgcac gccgatgagc cgtccgctac cttcctcgat
    13141 gaccagcttg atgaagccgc gtgtgtcgaa gttggcaagc gcacgcggca cgttatccag
    13201 tgttagcagg cgactgtcgg tctcgatccc gtcgtgatgt gcttccgcct cgctgtagcc
    13261 cacggtggcg acctgcgggt cggtgaacac cacggccggc attgcggtca ggtccagggc
    13321 cgcatcgccg ccagtcatgt tgatcgccgc acgagtgccg gccgctgccg ccacatagac
    13381 gaactgcggc tggtcggtgc agtcgccggc cgcgtagatg ttcgggctac tggtgcgcat
    13441 gcccttgtcg atgacgatgg ccccctgcgc attgacggct acccccgccg cttccaatgc
    13501 caggctgcgc gtgttcggtg tccggccggt ggcgaccagc agcttgtcgg cgcgcaattc
    13561 accgtgcgtg gtggtcagca cgaattcacc gtccatatgg gcgacctggc tggcttgcgt
    13621 gtgctccagc acctcgatgc cctcggcacg gaaagcggct gtcaccgcct cgccgatggc
    13681 cgggtcttca cggaagaaca aggtattgcg cgccagggcc gtgaccttgc tgcccagccg
    13741 ggcaaaggct tgcgccagct ccagcgccac caccgacgag ccgattacgg caaggcgttc
    13801 gggaatggtg tcgctcgcca gggcctcggt ggaagtccag tagggtgact ctttcaagcc
    13861 cggaatcggc gggaccgccg ggctggcacc cgtggcgacc aggcagcggt cgaacatcac
    13921 gacgcgctcg ccaccctcgt tcaaactaac gataaggctc tggtcgtcct tgaaacgcgc
    13981 ttcaccgtgc agaacggtga tggctgaatt gccgtccagg atgccttcgt acttggcatg
    14041 acggagttct tcgacacggg cctgctgctg ggccagcagc cgctcgcgca agatcgtcgg
    14101 cggtgtgggt ggcatgccgc cgtcgaatgg gctttcccgg cgcagatggg cgatgtgggc
    14161 ggcgcggatc atgatcttgg acggcacaca accgacgttg acgcaggtgc cgccgatggt
    14221 gccgcgctca atcagcgtga cctgcgcgcc ttgctcgacg gccttcagtg ctgccgccat
    14281 cgcggctcca ccgctaccaa tgacgacgac ctgcaacggg cgttcgttgc cactgggctt
    14341 atcagcggcc cctatccagc cgcgcatctt gtcgagcagg ccggcgcggt tgtccgtcgg
    14401 tggcgcatcg gcaagcgttg cctcgtagcc cagtccggcc acggcggtag tcagcgcatc
    14461 cgatgacgtg cccgcctcaa tggcgagttg cgctgtgccc ttcggatagg acaccagcgc
    14521 cgattgcacg ccgggcactt tctccaaggc ttccttgacg tgagccgcgc acgagtcgca
    14581 ggtcatcccg gtgattttca gggtggtcat gtatttttcc ttttctgtgg tggctacggc
    14641 tgttgccgtc agccacgttg ttctggcaat tcacagctgt ccggcccgca gcggcgatgt
    14701 gctggcgaga tgaaatccca gaccgacacc ccaaccatca aggccaggcc gacatagagc
    14761 agtccaccgc tctgccagcc gtaagcccgc attaaaaaca ccgctgccag caccaagatc
    14821 gggcctatcg tgccgagcgc cgtgcgtcgc cactgtcgat gattgagcca agcgatagca
    14881 ttggcgagta acgcgatgcc ggcgaacatc ggcagcagga tgccaatgaa tagcccctcg
    14941 tactggctca agaagcccag tccgatggcc gcgccaaagc tggcgatggc aggaaaacag
    15001 gcggcgcagc ccatcgcgga aacgacgctg ccgagcgcgc cggttttgcc agcgatgcgc
    15061 gtgatgagtc ccatgtgtcg ctcccgagtt cggttaacgg atcagcgttt gaggctggac
    15121 ggatagcccg cgtcctcggt cgccttggtc aacttctgga cgttggtctt ggcatcgtcg
    15181 aaggtgacga cggcctggcg cttgtcgaaa cttacgtcgg tcttgctcac gccctcaacc
    15241 ttggaaagcg cgtgcttgac agtgatcggg caagaggcgc aggtcatgcc aggcacggac
    15301 agcgtgacgg tctgggtggc ggcccacacg ggggcaacaa cggcagcgag ggcgagggcg
    15361 gcaaacagtt ttttcatgat gaactcctgt gattaataga aaaatggcat gacgtaggga
    15421 aatccgagcg agaccaggac cagcgcggcc acgatccaga aaatgagctt gtaagtagct
    15481 cgcacttggg gaatcgcgca gacctcaccc ggtttgcagg cttgcgccgg gcggtagatg
    15541 cgccgccagg cgaaaaacag cgcgaccagc gctgcgccga tgaagatcgg gcgatagggt
    15601 tccagcaccg tcaggttgcc gatccatgcc ccgctgaacc ccaaggcgat caaaaccagc
    15661 ggccccaggc agcaggccga cgcaagaatg gcggccagcc caccggcgaa gagcgcgccg
    15721 cgcccgtttt gtggttcaga cttttgtggt tcagacatac gcttgtcctt tcaaatttgg
    15781 tttggatagc ttaagcttac ttccgtagtt atgtacggag tcaagcgata tgcaaattaa
    15841 ttttgagaat ctgaccattg gcgtttttgc caaggcggcc ggggtcaatg tggagaccat
    15901 ccggttctac cagcgcaagg gcctgctgcc ggagccagac aagccctatg gcagcattcg
    15961 ccgctatggc gaggcggatg taacacgagt gcggttcgtg aaatcggccc agcggctggg
    16021 ctttagcctg gacgaaatcg ccgagctact gcggctggag gatggcaccc attgcgagga
    16081 agccagcggc ctggccgagc acaagctcaa ggatgtgcgc gagaagatgg ccgacttggc
    16141 acgcatggag gccgtgctgt ctgaactggt gtgcgcctgc catgcgcgga aagggaacgt
    16201 ttcctgcccg ctgattgcgt cactgcaaga cggaacgaag ctcgctgcat cggcgcgggg
    16261 gagtcacggg gtgactacgc cttagcgtgc tttattttcc gaattctgag acgacccc
//
