LOCUS       Tn1548                 16618 bp    DNA     linear   BCT 29-SEP-2020
DEFINITION  Escherichia coli.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Escherichia.
REFERENCE   1  (bases 1 to 16618)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn1548
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16618)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..16618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     repeat_region   1..14
                     /gene="IRL_IS26"
     CDS             64..768
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS26 transposase"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     repeat_region   807..820
                     /gene="IRR_IS26"
     repeat_region   841..865
                     /gene="IRi_In27"
     CDS             complement(1043..2056)
                     /gene="intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="Integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     misc_recomb     2137..2199
                     /gene="attI1"
     CDS             2201..2698
                     /gene="dfrA12"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydrofolate reductase"
                     /translation="MNSESVRIYLVAAMGANRVIGNGPNIPWKIPGEQKIFRRLTEGK
                     VVVMGRKTFESIGKPLPNRHTLVISRQANYRATGCVVVSTLSHAIALASELGNELYVA
                     GGAEIYTLALPHAHGVFLSEVHQTFEGDAFFPMLNETEFELVSTETIQAVIPYTHSVY
                     ARRNG"
     misc_recomb     2693..2782
                     /gene="attC_dfrA12"
     CDS             2810..3100
                     /gene="gcuF"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MFIQTAFSFSGVIQCLFCLFSGLRLHGLRRFSVFLASSPCVASA
                     SSYRFCSAVPPRWRSVFSRLAPVAKFKLSVLASGSNISVKPTRILRSAYLAR"
     misc_recomb     3043..3102
                     /gene="attC_gcuF"
     CDS             3106..3897
                     /gene="aadA2"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-adenyltransferase"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQ
                     DLFEALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
     misc_recomb     3899..3958
                     /gene="attC_aadA2"
     CDS             4061..4408
                     /gene="qacED1"
                     /codon_start=1
                     /transl_table=11
                     /product="Quaternary ammonium compound resistance protein"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     CDS             4402..5241
                     /gene="sul1"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydropteroate synthase"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     CDS             5646..7187
                     /gene="tnpA_ISCR1"
                     /codon_start=1
                     /transl_table=11
                     /product="tnpA for ISCR1"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASL
                     EAQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGAR
                     RMAESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIK
                     KAGYTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYD
                     ELNTLAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGP
                     QQGRKVFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPA
                     VSEKRLALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVF
                     APNSKHRVQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVC
                     EHCGGHVKVIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQ
                     SRLFD"
     repeat_region   7399..7419
                     /gene="oriIS_ISCR1"
     misc_feature    7424..8260
                     /gene="truncated tnpA"
                     /product="Truncated ISEc28 transposase"
     repeat_region   8298..8316
                     /gene="IRR_ISEc28"
     CDS             8586..9359
                     /gene="armA"
                     /codon_start=1
                     /transl_table=11
                     /product="Ribosomal RNA methyltransferase"
                     /translation="MDKNDVVKKILESKKYENLDSDIVEKVVSISEKKYKLKEVENYS
                     KKKLHQIWGSYYSAYPNWDKLLKKYNQGQLSIEDLLKIHSSTNERVATLNDFYTYVFG
                     NIKHVSSILDFGCGFNPLALYQWNENEKIIYHAYDIDRAEIAFLSSIIGKLKTTIKYR
                     FLNKESDVYKGTYDVVFLLKMLPVLKQQDVNILDFLQLFHTQNFVISFPIKSLSGKEK
                     GMEENYQLWFESFTKGWIKILDSKVIGNELVYITSGFQK"
     CDS             complement(9340..9621)
                     /gene="mnmA"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /translation="MLYVVQGKDNPKLWKNIVSVSELHLINETSLLNNNYTASIRYRS
                     QDTPVKVTQNENGYIFEFSAPQWAPAVGQSLVLFQENECLGGGVISEIH"
     repeat_region   9969..9989
                     /gene="IRL_ISEc29"
     CDS             10075..11259
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISEc29 transposase"
                     /translation="MLTMNVKAMLADFLTFVTPKSMHKARFSVLLDAVTALAKDACCT
                     VTAIGRAMPGSSDKVSIKRADRLLNNPNLQRELPLIYAALTASIVGHKTKPMILVDWS
                     NADTAKRHFILRASIAADGRALTLLQKIAAAEDYTCPHLHGAFLKQLKAMLPKDCKPV
                     IVTDAGFKVPWLKQVRKLGWHYVARVRGNVKLKLAEQDKFISVNQLYRQAKKDPKSVG
                     KIMLAQTQHYETQAVLVGKGYKLLKRDKNKTYKEPWLLVSSLADCHGYADKIAKCYSS
                     RMQIEESFRDQKSHRYGLGSDLHGTKKKSRLEILLLLAALVNWFHYLLGSAAEKAGLH
                     LRYQANTVKNRRVLALNFLGILLCKEPKQRIRRQYYQQGLKQILQWVVQWDWAVIKQA
                     DS"
     repeat_region   11273..11293
                     /gene="IRR_ISEc29"
     CDS             11658..13133
                     /gene="msr(E)"
                     /codon_start=1
                     /transl_table=11
                     /product="Macrolide efflux protein"
                     /translation="MSLIIKARNIRLDYAGRDVLDIDELEIHSYDRIGLVGDNGAGKS
                     SLLKVLNGEIVLAEATLQRFGDFAHISQLGGIEIETVEDRAMLSRLGVSNVQNDTMSG
                     GEETRAKIAAAFSQQVHGILADEPTSHLDLNGIDLLIGQLKAFDGALLVISHDRYFLD
                     MVVDKIWELKDGKITEYWGGYSDYLRQKEEERQHQAVEYELMMKERERLESAVQEKRQ
                     QANRLDNKKKGEKSKNSTESAGRLGHAKMTGTKQRKLYQAAKSMEKRLAALEDIQAPE
                     HLRSIRFRQSSALELHNKFPITADGLSLKFGSRTIFDDANFIIPLGAKVAITGSNGTG
                     KTSLLKMISERADGLTISPKAEIGYFTQTGYKFNTHKSVLSFMQEECEYTVAEIRAVL
                     ASMGIGANDIQKNLSDLSGGEIIKLLLSKMLLGKYNILLMDEPGNYLDLKSIAALETM
                     MKSYAGTIIFVSHDKQLVDNIADIIYEIKDHKIIKTFERDC"
     CDS             13189..14073
                     /gene="mph(E)"
                     /codon_start=1
                     /transl_table=11
                     /product="Macrolide 2'-phosphotransferase"
                     /translation="MTIQDIQSLAEAHGLLLTDKMNFNEMGIDFKVVFALDTKGQQWL
                     LRIPRRDGMREQIKKEKRILELVKKHLSVEVPDW*ISSTELVAYPILKDNPVLNLDAE
                     TYEIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLV
                     KSEIGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAH
                     IDDPAIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFAL
                     ETQNESLIVGAKAQLGVI"
     CDS             complement(14432..14974)
                     /gene="orf543"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MKKLSVSELAKLYGYSRQAIYAHINKGNLSKGSDGLIDFSEALR
                     VFGEPQKKEDTVNQSQSINSQNLTEVDLLKRQVDILEKQLNQAIQRENQSLERESFYQ
                     EQIEAMQRLLEAPKANMTTFTDQSFKQDIATDPRSELATNYDELTTPQQDNKRIPIPE
                     HVEPEQKKRGFLSRFFLPYG"
     CDS             complement(15041..15868)
                     /gene="repAciN"
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid replication protein RepA"
                     /translation="MGSCAAPSAKGDDKFITTDYLQQCRVHRNTAYQALKDACDDLFA
                     RQFSYQSLSEKGNTINHKSRWVSEVAYIDNEAVVRLIFAPAIVPLITRLEEQFTKYEI
                     QQISNLTSAYAVRLYEILIAWRSTGKTPLITMYDFRQKIGVLETEYKRMYDFKKYVLD
                     IALKQVNEHTDIIVKVEQHKTGRSITGFSFSFKQKKSATHSVESKRDPNTLDLFSKIT
                     DKQRHLFANKLSELPEMSKYSQGTESYQQFAVRIAAMLQDAEKFKELLPLLRKLGFQ"
     repeat_region   15796..15809
                     /gene="IRL_IS26"
     CDS             15859..16563
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS26 transposase"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     repeat_region   16602..16615
                     /gene="IRR_IS26"
ORIGIN      
        1 ggcactgttg caaatagtcg gtggtgataa acttatcatc cccttttgct gatggagctg
       61 cacatgaacc cattcaaagg ccggcatttt cagcgtgaca tcattctgtg ggccgtacgc
      121 tggtactgca aatacggcat cagttaccgt gagctgcagg agatgctggc tgaacgcgga
      181 gtgaatgtcg atcactccac gatttaccgc tgggttcagc gttatgcgcc tgaaatggaa
      241 aaacggctgc gctggtactg gcgtaaccct tccgatcttt gcccgtggca catggatgaa
      301 acctacgtga aggtcaatgg ccgctgggcg tatctgtacc gggccgtcga cagccggggc
      361 cgcactgtcg atttttatct ctcctcccgt cgtaacagca aagctgcata ccggtttctg
      421 ggtaaaatcc tcaacaacgt gaagaagtgg cagatcccgc gattcatcaa cacggataaa
      481 gcgcccgcct atggtcgcgc gcttgctctg ctcaaacgcg aaggccggtg cccgtctgac
      541 gttgaacacc gacagattaa gtaccggaac aacgtgattg aatgcgatca tggcaaactg
      601 aaacggataa tcggcgccac gctgggattt aaatccatga agacggctta cgccaccatc
      661 aaaggtattg aggtgatgcg tgcactacgc aaaggccagg cctcagcatt ttattatggt
      721 gatcccctgg gcgaaatgcg cctggtaagc agagtttttg aaatgtaagg cctttgaata
      781 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ccctcggcta ccacctccat
      841 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
      901 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
      961 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
     1021 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
     1081 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
     1141 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
     1201 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
     1261 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
     1321 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
     1381 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
     1441 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
     1501 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
     1561 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
     1621 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
     1681 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
     1741 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
     1801 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     1861 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     1921 ggatgaaggc acgaacccag tggacataag cctgttcggt tggtaagctg taatgcaagt
     1981 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     2041 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat
     2101 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2161 ttacgcagca gggcagtcgc cctaaaacaa agttagccat atgaactcgg aatcagtacg
     2221 catttatctc gttgctgcga tgggagccaa tcgggttatt ggcaatggtc ctaatatccc
     2281 ctggaaaatt ccgggtgagc agaagatttt tcgcagactc actgagggaa aagtcgttgt
     2341 catggggcga aagacctttg agtctatcgg caagcctcta ccgaaccgtc acacattggt
     2401 aatctcacgc caagctaact accgcgccac tggctgcgta gttgtttcaa cgctgtcgca
     2461 cgctatcgct ttggcatccg aactcggcaa tgaactctac gtcgcgggcg gagctgagat
     2521 atacactctg gcactacctc acgcccacgg cgtgtttcta tctgaggtac atcaaacctt
     2581 cgagggtgac gccttcttcc caatgctcaa cgaaacagaa ttcgagcttg tctcaaccga
     2641 aaccattcaa gctgtaattc cgtacaccca ctccgtttat gcgcgtcgaa acggctaacc
     2701 attccgtcaa cgggacgcca aaatgctgcg cattttggtt ccctccgctg cgctccggct
     2761 ctcgttacgt ccaacgttag caccactgaa acccagcttt atttagctca tgtttattca
     2821 aacggcattt agcttttcag gcgttattca gtgcctgttt tgcctttttt ccgggcttcg
     2881 cctgcatggg ctgcgcaggt tttcagtctt tttggcctct agcccttgcg tagcaagcgc
     2941 aagcagctat cgtttttgca gtgctgtgcc gcctcggtgg cgcagcgttt tttcacggtt
     3001 agcgcccgtc gccaaattca agttatccgt tttggcttct ggttctaaca tttcggtcaa
     3061 gccgacccgc attctgcggt cggcttacct cgcccgttag acatcatgag ggaagcggtg
     3121 accatcgaaa tttcgaacca actatcagag gtgctaagcg tcattgagcg ccatctggaa
     3181 tcaacgttgc tggccgtgca tttgtacggc tccgcagtgg atggcggcct gaagccatac
     3241 agcgatattg atttgttggt tactgtggcc gtaaagcttg atgaaacgac gcggcgagca
     3301 ttgctcaatg atcttatgga ggcttcggct ttccctggcg agagcgagac gctccgcgct
     3361 atagaagtca cccttgtcgt gcatgacgac atcatcccgt ggcgttatcc ggctaagcgc
     3421 gagctgcaat ttggagaatg gcagcgcaat gacattcttg cgggtatctt cgagccagcc
     3481 atgatcgaca ttgatctagc tatcctgctt acaaaagcaa gagaacatag cgttgccttg
     3541 gtaggtccgg cagcggagga attctttgac ccggttcctg aacaggatct attcgaggcg
     3601 ctgagggaaa ccttgaagct atggaactcg cagcccgact gggccggcga tgagcgaaat
     3661 gtagtgctta cgttgtcccg catttggtac agcgcaataa ccggcaaaat cgcgccgaag
     3721 gatgtcgctg ccgactgggc aataaaacgc ctacctgccc agtatcagcc cgtcttactt
     3781 gaagctaagc aagcttatct gggacaaaaa gaagatcact tggcctcacg cgcagatcac
     3841 ttggaagaat ttattcgctt tgtgaaaggc gagatcatca agtcagttgg taaatgatgt
     3901 ctaacaattc gttcaagccg accgcgctac gcgcggcggc ttaactccgg cgttagatgc
     3961 actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta
     4021 agcgtgcata ataagcccta cacaaattgg gagatatatc atgaaaggct ggctttttct
     4081 tgttatcgca atagttggcg aagtaatcgc aacatccgca ttaaaatcta gcgagggctt
     4141 tactaagctt gccccttccg ccgttgtcat aatcggttat ggcatcgcat tttattttct
     4201 ttctctggtt ctgaaatcca tccctgtcgg tgttgcttat gcagtctggt cgggactcgg
     4261 cgtcgtcata attacagcca ttgcctggtt gcttcatggg caaaagcttg atgcgtgggg
     4321 ctttgtaggt atggggctca taattgctgc ctttttgctc gcccgatccc catcgtggaa
     4381 gtcgctgcgg aggccgacgc catggtgacg gtgttcggca ttctgaatct caccgaggac
     4441 tccttcttcg atgagagccg gcggctagac cccgccggcg ctgtcaccgc ggcgatcgaa
     4501 atgctgcgag tcggatcaga cgtcgtggat gtcggaccgg ccgccagcca tccggacgcg
     4561 aggcctgtat cgccggccga tgagatcaga cgtattgcgc cgctcttaga cgccctgtcc
     4621 gatcagatgc accgtgtttc aatcgacagc ttccaaccgg aaacccagcg ctatgcgctc
     4681 aagcgcggcg tgggctacct gaacgatatc caaggatttc ctgaccctgc gctctatccc
     4741 gatattgctg aggcggactg caggctggtg gttatgcact cagcgcagcg ggatggcatc
     4801 gccacccgca ccggtcacct tcgacccgaa gacgcgctcg acgagattgt gcggttcttc
     4861 gaggcgcggg tttccgcctt gcgacggagc ggggtcgctg ccgaccggct catcctcgat
     4921 ccggggatgg gatttttctt gagccccgca ccggaaacat cgctgcacgt gctgtcgaac
     4981 cttcaaaagc tgaagtcggc gttggggctt ccgctattgg tctcggtgtc gcggaaatcc
     5041 ttcttgggcg ccaccgttgg ccttcctgta aaggatctgg gtccagcgag ccttgcggcg
     5101 gaacttcacg cgatcggcaa tggcgctgac tacgtccgca cccacgcgcc tggagatctg
     5161 cgaagcgcaa tcaccttctc ggaaaccctc gcgaaatttc gcagtcgcga cgccagagac
     5221 cgagggttag atcatgccta gcattcacct tccggccgcc cgctaaatat ctccttttgg
     5281 gttgttaata aaacatccaa taagttgact gtgcgtgaaa aagaaagttt tgtgtgatgg
     5341 cgttgaagat cgcaccgtta agctcttatg tgggatggtg cagagctcga cgactaccga
     5401 taaaacgcaa ccgccgcaaa cagacaagaa aaagccccaa ctgataacag ttggggcttc
     5461 agtattgtga ttggtggagc aatagcaccc tgaacccaaa accttctcgc tcaaccggta
     5521 gtggctgata acaactcgtg agggctattg cgggttaagc atttagcgat gtctagggcc
     5581 agactggacg tctgaacgca agccgctgat actgtacata accacagtat cagcggagga
     5641 tacccatgtc gctggcaagg aacgccacgg cgagtcaatc gcccactcaa acaaacggtt
     5701 acgaacgcca ccaacccgac cagacgctgc tctaccagct ggttgagcag cactacccag
     5761 ccttcaaagc ctcactcgaa gcccaaggtc aacacctgcc tcgctacatc caacaagaat
     5821 tcaacgacct cctccaatgt ggccgtctgg agtatggttt catgcgggtt cgctgcgagg
     5881 attgtcatca cgagcgtctg gtcgccttca gctgtaaacg acgcggcttt tgccctagct
     5941 gcggtgcccg ccggatggcc gagagtgcgg cgctgctgat agacgaagtc ttccccaagg
     6001 agcccattcg ccagtgggtg ctcagctttc ctttccagct acgctttttg ctggctcgcc
     6061 atccccagct gatgggccag gtcttgagta tcgtctatcg tacactctca actcatctga
     6121 tcaaaaaagc cggttacacc aaagcctctg cacaaactgg ctcagtgact cttatccaac
     6181 gctttggctc cgcgctaaat ctcaatgtcc actaccacat gctgtttctc gatggtgtct
     6241 atgccgaaga tgactatggc aagcaacgct tccatcgtgt caaggcaccc acttacgatg
     6301 agctgaatac gctcgctcac accctcagcc atcgcatcgc tcgctgcatg gaaaagcgtg
     6361 ggattttgga gcgtgatgcc gagaatacgt ggttgacact ggaagagggc gaagacgata
     6421 cgctgactca attacatggt gcttcggtta cgtatcgcat tgccgtcggc ccccagcaag
     6481 ggcgcaaagt cttcaccctg caaaccttgc cagggcgtga ggataaagcc gactcaagca
     6541 gtcgagtagc caaccatgct ggtttctcgc tacacgccgg tgtgatggcc gaagcgcatc
     6601 agcgggataa gcttgagcgc ttgtgtcgct acattagtcg gccagcggtt tcagaaaaac
     6661 gtctggcatt aaccgccaat gggcaggtgc gttacgagct caaaactccg taccgcaatg
     6721 gcaccaccca tgtgatcttc gagccgctgg acttcatcgc caaactcgct gcgttggtac
     6781 ctaagccgcg agtcaacctc acacgcttcc acggcgtctt tgcaccgaac agcaaacacc
     6841 gagttcaagt aacacccgcc aagcggggca agaagcccga caaatcggaa ggtctcgata
     6901 ctaactggcg tgacaagagt cctgcagagc gccaccgcgc catgacctgg atgcaacgcc
     6961 tcaagcgagt cttcaatatt gatattgaag tctgcgaaca ctgcggcggt cacgtcaaag
     7021 tgattgccag catcgaagat ccgaaggtca ttgagcagat tctcaagcat ctgaaacaga
     7081 aaacagccaa ggcgaatgcc gccaagcagc gtgagctgcc accagaacga gcgccgccac
     7141 tgactcccag cctgttcgat ccatcacaga gtcgtctctt tgactgacga ccccaaatcc
     7201 aacactgctc aacactgcca acttttaaac ggggcggtgg ggcagtttgt atctctcgag
     7261 ctatcaggct agagatttta ccgccaaatc gaaccttatt agagcggttt aggctggacc
     7321 ggcagttaaa attggggctt gagcggtaaa cgagtgaggg aatttcaggt aagatacttc
     7381 ggatgaggag caaaaaggtg gtttatactt cctatacccc tggatggacg aacaagccat
     7441 taagcagtgg cattgcctgg tccaccatgg ccgccgaggg agaggtttcc actacagtga
     7501 caccgccatt gagacggccc tgatgctaaa aggcttgttc aagttgccgc tgcgggcgct
     7561 agaaggcttc attaactcgt tgttccaact gatggcggtg cctttgcagt cgccggacta
     7621 tagctgtatc agcaagcgcg ccaagaccgt cgacatcaag taccgcctcc ccagccaagg
     7681 cccggtggct cacttggtca ttgatgccac cggcctcaag gtctatggcg aaggcgaatg
     7741 gaaaattcgc aaacacggca aggagaagcg acgagtgtgg cgcaagctcc acttggcggt
     7801 ggatgccaca acccatgcgg tcatcgctgc cgaagtcagc ctggagacgg tggcggataa
     7861 cgaggtgtta ccgacgttgc tcaacccctt acgacgcaag ataaaacagg tcagcgccga
     7921 tggtgcgtat gacaccaagg cctgtcatgc gctactgaag aaaaaggggg cgaaagccac
     7981 cataccgccg agaaaaaatg cggcaccgtg ggaggaaggc catccccgca acgaggcggt
     8041 gacggcgctc aaggccggtg aactgaagca atggaagaaa gactccggct atcaccagcg
     8101 ctcgatagcc gagaccgcca tgtaccggtt caagcaactc atcgggccaa cgctgagcct
     8161 gcggaactac aacgcccagg tgggcgaaat cctggctggc gtgaaggtga tgaacaagct
     8221 gatagggctt ggtatgcctg ttcgccagcc agtgaattga gtggtatcaa cgggttgggg
     8281 aacggccatc cagcgacgga tttggtcaac aacgccgata acacctcgaa atcggagttt
     8341 atatggcttc gtgggggagt tatgtgtttg agctttgtcg cattaacgcg aatcaggggt
     8401 tgacggcaga aatggactgg aattacactt cttagagtcg gcgttgccgg aaaattctga
     8461 ttggattagt tgttcggggt gcgaaaacag tcgtagttcg ggggaaaaac cgaattttga
     8521 cccgaaaccg caaaaaaccc gaaatgacag ttcctatcaa atacttaact tgtaggttct
     8581 ttcaaatgga taagaatgat gttgttaaga agatacttga atcaaaaaag tacgaaaacc
     8641 ttgattcaga tattgttgaa aaggttgttt ccatttctga gaagaaatat aaattaaagg
     8701 aagttgagaa ttattctaaa aagaaattgc atcaaatatg ggggtcttac tattctgcct
     8761 atcctaattg ggataaatta ttaaaaaagt acaatcaggg gcagttatca atagaagatt
     8821 tactaaagat tcattcttcg acgaatgaaa gagtcgcaac attaaatgac ttttacactt
     8881 atgtatttgg aaatatcaaa catgtctcat ctattttaga ttttggttgt ggcttcaatc
     8941 cattagcttt ataccaatgg aatgaaaatg aaaaaataat atatcatgca tacgatattg
     9001 atagagctga gatagctttt ttgagtagca ttattgggaa gttaaagacg acgataaagt
     9061 ataggttttt gaataaagag agtgatgtct acaaaggtac ttatgatgta gtattccttt
     9121 taaagatgct tcctgtgcta aaacagcaag atgtaaatat cttggatttc ctacagcttt
     9181 ttcatactca aaactttgta atatcttttc caataaagtc tttatctgga aaggagaagg
     9241 gaatggaaga gaattaccag ctatggtttg aatcttttac aaaaggttgg ataaaaatcc
     9301 ttgattcgaa ggttataggg aatgagttag tatatattac tagtggattt cagaaataac
     9361 tccgcctcca aggcactcat tttcttggaa aagtactaaa ctctgtccaa cggctggagc
     9421 ccattgtggt gcagaaaatt caaatatata tccattttca ttctgggtta cttttactgg
     9481 tgtatcctga cttctataac ggatagatgc tgtatagtta ttattaagta atgaagtttc
     9541 gtttatcaag tgcaattctg aaacacttac tatgtttttc caaagttttg gattatcttt
     9601 tccttggaca acatagagga tattttgagg aaaagatagg tttcaactac agtttagaag
     9661 gttaaaaaca aatgcatgca tggctcaaaa gcaatctagg gaaggtgcga ataagcgggg
     9721 aaattcttct cggctgactc agtcatttca ttttttcctg tttgagccgt tttttctccc
     9781 gtaaatgcac tgtataccga tatgggcagg gcgaagcgct aaagtacaac aatcttattg
     9841 ttagctattg tcttggcgtt ccagaaaatc tttaaactca atagtataaa gccgtaacag
     9901 caccatggtt aacgcaaaaa taattggccc ataaatcaaa ccaattaaac caaagagttg
     9961 caaaccacct gaggtatccc cagaaattct gtagttaaaa taaaatgtta gttgattggg
    10021 gaacattcat ggcggttcct gatctgatca ttcgccaaaa ctcccacacc gcggatgctc
    10081 accatgaatg taaaagctat gctcgctgat ttcctcacct ttgtcacgcc aaaatcaatg
    10141 cataaagccc gattttcggt tttgctagat gcggtaaccg ccctggcaaa ggatgcgtgt
    10201 tgcaccgtta ctgcgattgg ccgggcgatg cctggctctt cagataaggt cagtatcaaa
    10261 cgggcagatc gtctactcaa taatcctaac ctgcaacgag agctgccatt gatttatgct
    10321 gcgctgacgg cttctattgt tggccataaa actaagccga tgattttggt tgactggagc
    10381 aatgccgata ctgccaagcg acactttatc ctgcgtgcca gcattgccgc tgacggtaga
    10441 gcgttgactc tgctacagaa gattgccgcc gcagaagatt atacctgccc acacctacac
    10501 ggggcgtttt taaagcagct taaagccatg ctacccaagg actgtaagcc cgtaattgtc
    10561 actgacgcgg ggtttaaagt tccatggctg aaacaagtgc gtaagctggg atggcattat
    10621 gtagctagag ttcgaggcaa tgtgaagctt aaattggcag agcaggacaa gtttatcagt
    10681 gttaatcagc tttatcggca agcgaagaag gatccaaaaa gtgtaggaaa aatcatgctt
    10741 gcccaaacac aacactatga aacgcaggcc gtcctggttg gcaaaggtta taagctattg
    10801 aaacgcgata aaaataagac gtataaggaa ccatggttgt tggtgtcatc cttagctgac
    10861 tgccatgggt atgcggataa aattgctaag tgctacagta gccgaatgca aattgaagaa
    10921 agcttccgtg atcagaaaag tcaccgctat ggcctgggta gcgatttgca tggtaccaag
    10981 aagaaatctc gcttagagat actgctacta ctggccgcat tggttaattg gtttcattac
    11041 ctgctaggta gcgcagcgga gaaagcgggt ttgcacctgc gttatcaagc taacaccgtt
    11101 aaaaataggc gggtattggc cctgaatttc cttgggatat tactttgcaa agaacccaaa
    11161 cagcgaatac gcaggcaata ttatcagcag ggacttaaac aaatactaca gtgggtggtt
    11221 cagtgggact gggcagtaat caaacaggct gatagctgat tgtatgaatg gaaattttgt
    11281 ggggatccct caggttgtct agacttcttt taacagtaaa gttatcataa aactgaattt
    11341 tattttttag gtaagtttat gcattctatc cgcattcgtt aagacacaac tatttgcata
    11401 gtgacactat tttataatgg tgggcttttg ttgtgtcttt aagaatatat gcggatatat
    11461 aaagtaaaag tatgcttaat ttataagtat gcttttagtg catagtttcc agttataact
    11521 taattgacta gctatttgtc caccctgtgg atgaatagct ttttttttgg gaggacactg
    11581 tgatgctagc ttttgttttc acctaaatcc tgtttgctgc ataaaaaatt tcaagagcta
    11641 aacaggagta aataaaaatg agtttaatta ttaaagcgag aaacatacgc ttggattatg
    11701 ctgggcgtga tgttttggat attgatgaat tggaaattca ctcttatgac cgtattggtc
    11761 ttgtgggtga taacggagca ggaaagagta gtttactcaa agtacttaat ggcgaaattg
    11821 ttttagccga agcgacatta cagcgttttg gtgattttgc acatatcagc caactgggcg
    11881 gaatcgaaat agaaacggtc gaagaccggg caatgttatc tcgccttggt gtttccaatg
    11941 tacaaaacga cacaatgagt ggcggagagg aaactcgtgc aaaaattgct gccgcatttt
    12001 cccaacaagt acatggcatt ctagcggatg aaccaaccag ccaccttgat ctcaatggaa
    12061 tagatctact tattggtcaa cttaaagcat ttgatggagc attacttgtt atcagtcatg
    12121 accgatattt tcttgatatg gttgtagaca agatatggga gttaaaagac ggtaaaatta
    12181 cggaatattg gggtggttac tcggattact tgcgtcaaaa agaagaagag cgacaacacc
    12241 aagccgtaga atatgagctg atgatgaagg aacgggagcg attagaatct gctgtgcaag
    12301 aaaaacgcca gcaagctaat cgattagaca ataagaaaaa aggagaaaaa tccaaaaact
    12361 ctaccgaaag tgctggacga cttgggcatg caaaaatgac tggcaccaag caaagaaaac
    12421 tgtatcaggc agctaagagt atggaaaagc gtttggctgc attagaagat attcaagcac
    12481 cagagcattt gcgttctatt cgttttcgtc aaagttcagc cctagaactg cacaataagt
    12541 tcccgattac ggcagatggt ctgagcttaa aatttggtag ccgtactatc tttgatgacg
    12601 ctaactttat aataccgctt ggcgctaaag tcgctataac tggatcgaat ggaacaggga
    12661 aaacgtcctt gttaaaaatg atatcagaac gtgctgatgg attaaccata tctccaaaag
    12721 ctgaaattgg ctactttaca caaacaggat ataaatttaa cacgcataaa tctgtgctct
    12781 cctttatgca ggaagagtgc gagtacacag ttgcggaaat tcgtgcagta ttggcttcaa
    12841 tggggatcgg agcgaatgat attcaaaaaa acttatccga cttatcggga ggtgaaatca
    12901 tcaaactgct tttatccaaa atgcttttag gaaaatataa tattttgctt atggatgaac
    12961 caggaaacta tcttgaccta aaaagtattg ccgcattaga aacaatgatg aagtcctatg
    13021 caggaactat tatcttcgta tctcatgaca agcaattggt cgataatatt gctgacatta
    13081 tctacgagat caaagaccac aaaatcatca agacttttga gagagattgt taatgatagc
    13141 caatctaatc cgaacattaa ttattgaact ctttaaagga aattaaaaat gacaattcaa
    13201 gatattcaat cacttgctga agcacacggc ttgttgctta cggacaaaat gaatttcaat
    13261 gaaatgggca ttgattttaa ggtcgttttt gctcttgata caaaggggca acaatggttg
    13321 ctgcgtattc ctcgtcgtga tggcatgagg gaacaaatca agaaagaaaa acgcatttta
    13381 gaattggtaa aaaaacatct ttctgtagag gttcctgatt ggtgaatttc atctacagaa
    13441 ttagtggctt atcccatact taaagataat cctgttttaa atttggatgc tgaaacctat
    13501 gaaataattt ggaatatgga caaagatagc ccgaaataca taacatcttt ggcaaaaacc
    13561 ttatttgaaa tccatagtat tcctgaaaaa gaagttcggg aaaatgattt gaaaattatg
    13621 aaaccttcag atttaagacc tgaaatagca aacaatttgc agttagtaaa atctgaaatt
    13681 ggtataagtg agcaattgga aacccgctac agaaaatggt tggataatga tgttctatgg
    13741 gcagatttca cccaatttat acatggcgat ttatatgctg ggcatgtact agcttcaaag
    13801 gatggagctg tttcaggcgt tattgattgg tcaacagccc atatagatga cccagcgatt
    13861 gattttgctg ggcatgtaac tttgtttgga gaagaaagcc tcaaaactct aatcatcgag
    13921 tatgaaaaac tagggggtaa agtttggaat aaactatatg aacagacttt agaaagagca
    13981 gcggcctctc ctttgatgta tggtttattt gccttagaaa ctcaaaatga aagccttatc
    14041 gttggagcaa aagctcagtt gggagttata taatttaaaa atatgattgc tgagaactgc
    14101 cttgttttga aacttggttg gctttaatta gtttttagta ttctttatag aaaatgcctc
    14161 gatcaagggg catttctaac aatcatttaa cataaaattt cttatacgaa atgcttgata
    14221 tttctcttta aatatcatta tattaacgta agccgttctg gagtataggg caccagaacg
    14281 gctttttatt gattattcat gttccacgat cactaattga gcgatgttcc acggttttat
    14341 tcagttttaa taaacatcat ccggtttttt gaggagtcat tatttcaagt gcttgcactg
    14401 aaattggctc ttcaaaaaac tggagtcttg gctaaccata tggaaggaaa aagcggctta
    14461 ggaagcctct cttcttctgt tctggttcaa catgctccgg gataggaata cgcttattgt
    14521 cttgctgagg agtagtcaat tcgtcatagt tcgttgctag ttccgaccga ggatccgttg
    14581 ctatatcctg tttaaagctc tgatcggtaa aggtagtcat attggctttt ggtgcttcta
    14641 gtaagcgctg catagcttca atctgttctt gataaaacga ttcacgttct aaagattgat
    14701 tttctctctg tattgcctga tttaattgtt tttccagtat gtcaacttga cgttttagta
    14761 agtcaacttc tgtcaagttt tgactgttaa ttgattgact ttgattgact gtatcctctt
    14821 ttttttgtgg ttccccaaaa actcttaggg cctctgaaaa gtcaattaat ccatcagatc
    14881 ctttagataa atttcctttg tttatatgtg catatatggc ttgtcttgaa tatccataaa
    14941 gcttagctaa ctctgaaact gacagttttt tcattatgta aaccagtttt caacttgtgt
    15001 taatacctga ctgttaactt aacaatttca attaacaatg tcattgaaat cctaattttc
    15061 tgagtaaagg aagtaattcc ttaaatttct ctgcatcttg cagcatggca gctatacgta
    15121 cagcaaactg ctgatagctt tctgtgcctt gtgaatattt actcatctca ggaagctctg
    15181 agagcttgtt ggcgaataga tggcgttgtt tatctgttat ttttgaaaag aggtctaatg
    15241 tattcggatc tcttttagat tcgactgaat gcgtggctga ttttttctgt ttaaagctaa
    15301 atgaaaaacc agtaatagat ctacctgttt tatgctgttc aactttgaca ataatatcgg
    15361 tatgttcatt tacttgtttt aatgcaatgt ctaaaacata ttttttaaaa tcatacattc
    15421 gtttgtattc agtctcgagt acacctattt tttgtctaaa atcatacata gttatgagag
    15481 gcgtttttcc agtactacgc catgcaatca atatttcata taaacgaaca gcataagcac
    15541 ttgttaaatt acttatttgt tgtatttcat actttgtaaa ttgttcttct aacctagtaa
    15601 ttaaaggcac aatagcaggg gcaaaaataa gtctaacgac agcttcatta tcaatataag
    15661 ccacctcgct cacccatctt gatttgtgat taatagtgtt acctttttca ctaagactct
    15721 gataactgaa ttgtcttgca aacaagtcat cgcaagcatc ttttaacgcc tgataagccg
    15781 tatttcgatg tacacggcac tgttgcaaat agtcggtggt gataaactta tcatcccctt
    15841 ttgctgatgg agctgcacat gaacccattc aaaggccggc attttcagcg tgacatcatt
    15901 ctgtgggccg tacgctggta ctgcaaatac ggcatcagtt accgtgagct gcaggagatg
    15961 ctggctgaac gcggagtgaa tgtcgatcac tccacgattt accgctgggt tcagcgttat
    16021 gcgcctgaaa tggaaaaacg gctgcgctgg tactggcgta acccttccga tctttgcccg
    16081 tggcacatgg atgaaaccta cgtgaaggtc aatggccgct gggcgtatct gtaccgggcc
    16141 gtcgacagcc ggggccgcac tgtcgatttt tatctctcct cccgtcgtaa cagcaaagct
    16201 gcataccggt ttctgggtaa aatcctcaac aacgtgaaga agtggcagat cccgcgattc
    16261 atcaacacgg ataaagcgcc cgcctatggt cgcgcgcttg ctctgctcaa acgcgaaggc
    16321 cggtgcccgt ctgacgttga acaccgacag attaagtacc ggaacaacgt gattgaatgc
    16381 gatcatggca aactgaaacg gataatcaac gccacgctgg gatttaaatc catgaagacg
    16441 gcttacgcca ccatcaaagg tattgaggtg atgcgtgcac tacgcaaagg ccaggcctca
    16501 gcattttatt atggtgatcc cctgggcgaa atgcgcctgg taagcagagt ttttgaaatg
    16561 taaggccttt gaataagaca aaaggctgcc tcatcgctaa ctttgcaaca gtgccctt
//
