LOCUS       Tn1546                 10851 bp    DNA     linear       29-SEP-2020
DEFINITION  Enterococcus faecium.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Unclassified.
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn1546
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10851)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..10851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
     repeat_region   1..38
                     /gene="IRL_Tn1546"
     CDS             complement(75..3041)
                     /gene="tnpA"
                     /codon_start=1
                     /product="Tn1546 transposase"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
     misc_recomb     3073..3187
                     /gene="res"
     CDS             3187..3762
                     /gene="tnpR"
                     /codon_start=1
                     /product="Tn1546 resolvase"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     CDS             3976..4671
                     /gene="vanR"
                     /codon_start=1
                     /product="Vancomycin resistance DNA-binding response
                     regulator VanR"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     CDS             4661..5803
                     /gene="vanS"
                     /codon_start=1
                     /product="Sensor histidine kinase VanS"
                     /translation="MKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGKLGDW
                     ILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKYFDEI
                     NTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVMYLAH
                     DIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRYNLQT
                     ITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFNNILK
                     NAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSSDTGG
                     AGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     CDS             6018..6986
                     /gene="vanH"
                     /codon_start=1
                     /product="Vancomycin resistance protein VanH"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     CDS             6979..8010
                     /gene="vanA"
                     /codon_start=1
                     /product="Vancomycin/teicoplanin A-type resistance protein
                     VanA"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     CDS             8016..8624
                     /gene="vanX"
                     /codon_start=1
                     /product="D-alanyl-D-alanine dipeptidase"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     CDS             9052..9963
                     /gene="vanY"
                     /codon_start=1
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     CDS             10116..10601
                     /gene="vanZ"
                     /codon_start=1
                     /product="VanZ family protein"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     repeat_region   10814..10851
                     /gene="IRR_Tn1546"
ORIGIN      
        1 ggggtagcgt caggaaaatg cggatttaca acgctaagcc tattttcctg acgaatccct
       61 cgtttttaac aacgttaaga aagttttagt ggtcttaaag aatttaatga gactactttc
      121 tctgagttaa aatggtattc tcctagtaaa ttaatatgtt cccaacctaa gggcgacata
      181 tggtgtaaca aatcttcatt aaagctacct gtccgttttt tatattcaac tgctgttgtt
      241 aggtggagag tattccaaat acttatagca ttgataatta tgtttaaagc actggctctt
      301 tgcaattgat gctgtatggt gcgttctcta agctcacctt gttttccgaa gaaaatagct
      361 cttgccaatc cattcatggc ttctccttta ttcaatcctc tttgtatttt tcttcttaat
      421 gattcatccg atatataatt caaaataaag atcgtttttt ctattcggcc catctcacgt
      481 aaggctgtag ctaagctgtt ttgtcttgaa taggaaccta gcttccccat aataagggat
      541 gctgaaactg ttccctccct tatagaatga gctaatcgca aaacatcctc ataattttct
      601 ttaatgacct ttgtatttat ttgtccacgt aaaatggctt ctagttttgg atactcactt
      661 gctttatcta tcgtaaataa ttttgagtcc gataaatccc ttattcttgg ggcaaattta
      721 aatcctaata aatgagtcag tccgaatatt tggtcagtgt aaccggcagt gtctgtataa
      781 tgttcctcta tgtttagatc cgtctcatga tgtaacaaac catccaaaac atgaatcgca
      841 tctcttgaat tagtatgaat aatctttgtg tagtaagaag agaattgatc acttgtaaat
      901 cggtagatgg tggctccttt tccagttcca taatgtggat ttgcatctgc atgtagtgat
      961 gaaacaccta gctgcattct cataccatct gacgaagatg ttgtaccgtc gccccaatag
     1021 aaaggcaatt gtaatttatg atgaaagttt actaatatgg cttgggcttt attcatggca
     1081 tcttcataca tgcgccattg agatacattg gctagttgct tatatgtaag tccgggtgtg
     1141 gcttcggcca tcttgctcaa gccaatattc attcccattc ctaaaagggc agccatgata
     1201 atgattgttt cttccttatc tggttttcga ttattggaag catgagtgaa ttgctcatga
     1261 aatcctgtta tatgggccac atccatgagt aaatcagtta attttattct tggtagcatc
     1321 tgataaaggc ttgcactaaa tttttttgct tcttctggaa catctttttc taagcgtgca
     1381 agtgatagct ttcctttttc aagagaaacc ccatctaact tattggaatt ggcagctaac
     1441 cactttaacc tttcattaaa gctgctggtt ctctccgtta tataatcttc gaatgataaa
     1501 ctaactgata atctcgtatt ccccttcgat tgattccatg tatcttccga aaacaaatat
     1561 tcctcaaaat ccctatattg tctgctgcca acaatggaaa catctcctgc ccgaacatgc
     1621 tcccgaagtt ctgttaaaac agccatttca tagtaatgac gattaattgt tgtaccatca
     1681 tcctcgtata aatgtctttt ccatcgtttt gaaataaaat ccacaggtga gtcatcaggc
     1741 acttttcgct ttccagattc gttcattcct cggataatct caacagcttg taaaagtggc
     1801 tcatttgcct ttgtagaatg aaattccaat actcttaata gcgttggcgt atattttctt
     1861 agtgaataaa accgtttttg cagtaagtct aaataatcat agtcggcagg acgtgcaagt
     1921 tcctgagcct cttctactga agagacaaag gtattccatt caataaccga ttctaaaacc
     1981 ttaaaaacgt ctaatttttc ctctcttgct ttaattaatg cttgtccgat gttcgtaaag
     2041 tgtataactt tctcatttag ctttttaccg ttttgtttct ggatttcctc ttgagcctta
     2101 cgaccttttg ataacaaact aagtatttgc ctatcatgaa tttcaaacgc tttatccgtt
     2161 agctcctgag taagttgtaa taaatagatg gttaatatcg aataacgttt attttcttga
     2221 aagtcacgga atgcatacgg ctcgtatctt gagcctaagc gagacagctg caacaggcgg
     2281 ttacggtgca aatgactaat ttgcactgtt tctaaatcca ttcctcgtat gtattcgagt
     2341 cgttctatta tttttagaaa agtttcgggt gaaggatgac ccggtggctc ttttaaccaa
     2401 cccaatatcg ttttattgga ttcggatgga tgctgcgagg taataatccc ttcaagcttt
     2461 tctttttgct catttgttag agatttacta accgtattaa atagcttctt ttcagccatt
     2521 gcccttgctt cccacaccat tctttcaagt gtagtgatag caggcagtat aattttgttt
     2581 tttcttagaa aatctatgca ttcatgcagt agatgaatgg catcaccatt ttccaaagct
     2641 aattgatgaa ggtacttaaa tgtcattcga tattcactca gggtaaaagt tacaaagtcg
     2701 tattcacttc gaatttcttt caaatgatcc caaagtgtat tttccctttg aggataatga
     2761 tcaagcgagg atggactaac accaatctgt ttcgatatat attgtatgac cgaatctggg
     2821 atgcttttga tatgagtgta tggccaaccg ggataccgaa gaacagctaa ttgaacagca
     2881 aatcctaaac ggttttcttc cctccttcgc ttattaacta tttctaaatc ccgtttggaa
     2941 aaagtgaagt aggtccccag tatccattca tcttcaggga tttgcataaa agcctgtctc
     3001 tgttccggtg taagcaattc tctacctctc gcaattttca ttcagtatca ttccatttct
     3061 gtattttcaa tttattagtt caattatata tcaatagagt gtactctatt gatacaaatg
     3121 tagtagactg ataaaatcat agttaagagc gtctcataag acttgtctca aaaatgaggt
     3181 gatattttgc ggaaaatcgg ttatattcgt gtcagttcga ctaaccagaa tccttcaaga
     3241 caatttcagc agttgaacga gatcggaatg gatattatat atgaagagaa agtttcagga
     3301 gcaacaaagg atcgcgagca acttcaaaaa gtgttagacg atttacagga agatgacatc
     3361 atttatgtta cagacttaac tcgaatcact cgtagtacac aagatctatt tgaattaatc
     3421 gataacatac gagataaaaa ggcaagttta aaatcactaa aagatacatg gcttgattta
     3481 tcagaagata atccatacag ccaattctta attactgtaa tggctggtgt taaccaatta
     3541 gagcgagatc ttattcggat gagacaacgt gaagggattg aattggctaa gaaagaagga
     3601 aagtttaaag gtcgattaaa gaagtatcat aaaaatcacg caggaatgaa ttatgcggta
     3661 aagctatata aagaaggaaa tatgactgta aatcaaattt gtgaaattac taatgtatct
     3721 agggcttcat tatacaggaa attatcagaa gtgaataatt agccattctg tattccgcta
     3781 atgggcaata tttttaaaga agaaaaggaa actataaaat attaacagcc tcctagcgat
     3841 gccgaaaagc cctttgataa aaaaagaatc atcatcttaa gaaattctta gtcatttatt
     3901 atgtaaatgc ttataaattc ggccctataa tctgataaat tattaagggc aaacttatgt
     3961 gaaagggtga taactatgag cgataaaata cttattgtgg atgatgaaca tgaaattgcc
     4021 gatttggttg aattatactt aaaaaacgag aattatacgg ttttcaaata ctataccgcc
     4081 aaagaagcat tggaatgtat agacaagtct gagattgacc ttgccatatt ggacatcatg
     4141 cttcccggca caagcggcct tactatctgt caaaaaataa gggacaagca cacctatccg
     4201 attatcatgc tgaccgggaa agatacagag gtagataaaa ttacagggtt aacaatcggc
     4261 gcggatgatt atataacgaa gccctttcgc ccactggagt taattgctcg ggtaaaggcc
     4321 cagttgcgcc gatacaaaaa attcagtgga gtaaaggagc agaacgaaaa tgttatcgtc
     4381 cactccggcc ttgtcattaa tgttaacacc catgagtgtt atctgaacga gaagcagtta
     4441 tcccttactc ccaccgagtt ttcaatactg cgaatcctct gtgaaaacaa ggggaatgtg
     4501 gttagctccg agctgctatt tcatgagata tggggcgacg aatatttcag caagagcaac
     4561 aacaccatca ccgtgcatat ccggcatttg cgcgaaaaaa tgaacgacac cattgataat
     4621 ccgaaatata taaaaacggt atggggggtt ggttataaaa ttgaaaaata aaaaaaacga
     4681 ctattccaaa ctagaacgaa aactttacat gtatatcgtt gcaattgttg tggtagcaat
     4741 tgtattcgtg ttgtatattc gttcaatgat ccgagggaaa cttggggatt ggatcttaag
     4801 tattttggaa aacaaatatg acttaaatca cctggacgcg atgaaattat atcaatattc
     4861 catacggaac aatatagata tctttattta tgtggcgatt gtcattagta ttcttattct
     4921 atgtcgcgtc atgctttcaa aattcgcaaa atactttgac gagataaata ccggcattga
     4981 tgtacttatt cagaacgaag ataaacaaat tgagctttct gcggaaatgg atgttatgga
     5041 acaaaagctc aacacattaa aacggactct ggaaaagcga gagcaggatg caaagctggc
     5101 cgaacaaaga aaaaatgacg ttgttatgta cttggcgcac gatattaaaa cgccccttac
     5161 atccattatc ggttatttga gcctgcttga cgaggctcca gacatgccgg tagatcaaaa
     5221 ggcaaagtat gtgcatatca cgttggacaa agcgtatcga ctcgaacagc taatcgacga
     5281 gttttttgag attacacggt ataacctaca aacgataacg ctaacaaaaa cgcacataga
     5341 cctatactat atgctggtgc agatgaccga tgaattttat cctcagcttt ccgcacatgg
     5401 aaaacaggcg gttattcacg cccccgagga tctgaccgtg tccggcgacc ctgataaact
     5461 cgcgagagtc tttaacaaca ttttgaaaaa cgccgctgca tacagtgagg ataacagcat
     5521 cattgacatt accgcgggcc tctccgggga tgtggtgtca atcgaattca agaacactgg
     5581 aagcatccca aaagataagc tagctgccat atttgaaaag ttctataggc tggacaatgc
     5641 tcgttcttcc gatacgggtg gcgcgggact tggattggcg attgcaaaag aaattattgt
     5701 tcagcatgga gggcagattt acgcggaaag caatgataac tatacgacgt ttagggtaga
     5761 gcttccagcg atgccagact tggttgataa aaggaggtcc taagagatgt atataatttt
     5821 ttaggaaaat ctcaaggtta tctttacttt ttcttaggaa attaacaatt taatattaag
     5881 aaacggctcg ttcttacacg gtagacttaa taccgtaaga acgagccgtt ttcgttcttc
     5941 agagaaagat ttgacaagat taccattggc atccccgttt tatttggtgc ctttcacaga
     6001 aagggttggt cttaattatg aataacatcg gcattactgt ttatggatgt gagcaggatg
     6061 aggcagatgc attccatgct ctttcgcctc gctttggcgt tatggcaacg ataattaacg
     6121 ccaacgtgtc ggaatccaac gccaaatccg cgcctttcaa tcaatgtatc agtgtgggac
     6181 ataaatcaga gatttccgcc tctattcttc ttgcgctgaa gagagccggt gtgaaatata
     6241 tttctacccg aagcatcggc tgcaatcata tagatacaac tgctgctaag agaatgggca
     6301 tcactgtcga caatgtggcg tactcgccgg atagcgttgc cgattatact atgatgctaa
     6361 ttcttatggc agtacgcaac gtaaaatcga ttgtgcgctc tgtggaaaaa catgatttca
     6421 ggttggacag cgaccgtggc aaggtactca gcgacatgac agttggtgtg gtgggaacgg
     6481 gccagatagg caaagcggtt attgagcggc tgcgaggatt tggatgtaaa gtgttggctt
     6541 atagtcgcag ccgaagtata gaggtaaact atgtaccgtt tgatgagttg ctgcaaaata
     6601 gcgatatcgt tacgcttcat gtgccgctca atacggatac gcactatatt atcagccacg
     6661 aacaaataca gagaatgaag caaggagcat ttcttatcaa tactgggcgc ggtccacttg
     6721 tagataccta tgagttggtt aaagcattag aaaacgggaa actgggcggt gccgcattgg
     6781 atgtattgga aggagaggaa gagtttttct actctgattg cacccaaaaa ccaattgata
     6841 atcaattttt acttaaactt caaagaatgc ctaacgtgat aatcacaccg catacggcct
     6901 attataccga gcaagcgttg cgtgataccg ttgaaaaaac cattaaaaac tgtttggatt
     6961 ttgaaaggag acaggagcat gaatagaata aaagttgcaa tactgtttgg gggttgctca
     7021 gaggagcatg acgtatcggt aaaatctgca atagagatag ccgctaacat taataaagaa
     7081 aaatacgagc cgttatacat tggaattacg aaatctggtg tatggaaaat gtgcgaaaaa
     7141 ccttgcgcgg aatgggaaaa cgacaattgc tattcagctg tactctcgcc ggataaaaaa
     7201 atgcacggat tacttgttaa aaagaaccat gaatatgaaa tcaaccatgt tgatgtagca
     7261 ttttcagctt tgcatggcaa gtcaggtgaa gatggatcca tacaaggtct gtttgaattg
     7321 tccggtatcc cttttgtagg ctgcgatatt caaagctcag caatttgtat ggacaaatcg
     7381 ttgacataca tcgttgcgaa aaatgctggg atagctactc ccgccttttg ggttattaat
     7441 aaagatgata ggccggtggc agctacgttt acctatcctg tttttgttaa gccggcgcgt
     7501 tcaggctcat ccttcggtgt gaaaaaagtc aatagcgcgg acgaattgga ctacgcaatt
     7561 gaatcggcaa gacaatatga cagcaaaatc ttaattgagc aggctgtttc gggctgtgag
     7621 gtcggttgtg cggtattggg aaacagtgcc gcgttagttg ttggcgaggt ggaccaaatc
     7681 aggctgcagt acggaatctt tcgtattcat caggaagtcg agccggaaaa aggctctgaa
     7741 aacgcagtta taaccgttcc cgcagacctt tcagcagagg agcgaggacg gatacaggaa
     7801 acggcaaaaa aaatatataa agcgctcggc tgtagaggtc tagcccgtgt ggatatgttt
     7861 ttacaagata acggccgcat tgtactgaac gaagtcaata ctctgcccgg tttcacgtca
     7921 tacagtcgtt atccccgtat gatggccgct gcaggtattg cacttcccga actgattgac
     7981 cgcttgatcg tattagcgtt aaaggggtga taagcatgga aataggattt acttttttag
     8041 atgaaatagt acacggtgtt cgttgggacg ctaaatatgc cacttgggat aatttcaccg
     8101 gaaaaccggt tgacggttat gaagtaaatc gcattgtagg gacatacgag ttggctgaat
     8161 cgcttttgaa ggcaaaagaa ctggctgcta cccaagggta cggattgctt ctatgggacg
     8221 gttaccgtcc taagcgtgct gtaaactgtt ttatgcaatg ggctgcacag ccggaaaata
     8281 acctgacaaa ggaaagttat tatcccaata ttgaccgaac tgagatgatt tcaaaaggat
     8341 acgtggcttc aaaatcaagc catagccgcg gcagtgccat tgatcttacg ctttatcgat
     8401 tagacacggg tgagcttgta ccaatgggga gccgatttga ttttatggat gaacgctctc
     8461 atcatgcggc aaatggaata tcatgcaatg aagcgcaaaa tcgcagacgt ttgcgctcca
     8521 tcatggaaaa cagtgggttt gaagcatata gcctcgaatg gtggcactat gtattaagag
     8581 acgaaccata ccccaatagc tattttgatt tccccgttaa ataaactttt aaccgttgca
     8641 cggacaaact atataagcta actctttcgg caggaaaccc gacgtatgta actggttctt
     8701 agggaattta tatatagtag atagtattga agatgtaagg cagagcgata ttgcggtcat
     8761 tatctgcgtg cgctgcggca agatagcctg ataataagac tgatcgcata gaggggtggt
     8821 atttcacacc gcccattgtc aacaggcagt tcagcctcgt taaattcagc atgggtatca
     8881 cttatgaaaa ttcatctaca ttggtgataa tagtaaatcc agtagggcga aataattgac
     8941 tgtaatttac ggggcaaaac ggcacaatct caaacgagat tgtgccgttt aaggggaaga
     9001 ttctagaaat atttcatact tccaactata tagttaagga ggagactgaa aatgaagaag
     9061 ttgttttttt tattgttatt gttattctta atatacttag gttatgacta cgttaatgaa
     9121 gcactgtttt ctcaggaaaa agtcgaattt caaaattatg atcaaaatcc caaagaacat
     9181 ttagaaaata gtgggacttc tgaaaatacc caagagaaaa caattacaga agaacaggtt
     9241 tatcaaggaa atctgctatt aatcaatagt aaatatcctg ttcgccaaga aagtgtgaag
     9301 tcagatatcg tgaatttatc taaacatgac gaattaataa atggatacgg gttgcttgat
     9361 agtaatattt atatgtcaaa agaaatagca caaaaatttt cagagatggt caatgatgct
     9421 gtaaagggtg gcgttagtca ttttattatt aatagtggct atcgagactt tgatgagcaa
     9481 agtgtgcttt accaagaaat gggggctgag tatgccttac cagcaggtta tagtgagcat
     9541 aattcaggtt tatcactaga tgtaggatca agcttgacga aaatggaacg agcccctgaa
     9601 ggaaagtgga tagaagaaaa tgcttggaaa tacgggttca ttttacgtta tccagaggac
     9661 aaaacagagt taacaggaat tcaatatgaa ccatggcata ttcgctatgt tggtttacca
     9721 catagtgcga ttatgaaaga aaagaatttc gttctcgagg aatatatgga ttacctaaaa
     9781 gaagaaaaaa ccatttctgt tagtgtaaat ggggaaaaat atgagatctt ttattatcct
     9841 gttactaaaa ataccaccat tcatgtgccg actaatcttc gttatgagat atcaggaaac
     9901 aatatagacg gtgtaattgt gacagtgttt cccggatcaa cacatactaa ttcaaggagg
     9961 taaggatggc ggaatgaaac caacgaaatt aatgaacagc attattgtac tagcactttt
    10021 ggggtaacgt tagcttttta atttaaaacc cacgttaact aggacattgc tatactaatg
    10081 atacaactta aacaaaagaa ttagaggaaa ttatattggg aaaaatatta tctagaggat
    10141 tgctagcttt atatttagtg acactaatct ggttagtgtt attcaaatta caatacaata
    10201 ttttatcagt atttaattat catcaaagaa gtcttaactt gactccattt actgctactg
    10261 ggaatttcag agagatgata gataatgtta taatctttat tccatttggc ttgcttttga
    10321 atgtcaattt taaagaaatc ggatttttac ctaagtttgc ttttgtactg gttttaagtc
    10381 ttacttttga aataattcaa tttatcttcg ctattggagc gacagacata acagatgtaa
    10441 ttacaaatac tgttggaggc tttcttggac tgaaattata tggtttaagc aataagcata
    10501 tgaatcaaaa aaaattagac agagttatta tttttgtagg tatacttttg ctcgtattat
    10561 tgctcgttta ccgtacccat ttaagaataa attacgtgta agatgtctaa atcaagcaat
    10621 ctgatctttc atacacataa agatattgaa tgaattggat tagatggaaa acgggatgtg
    10681 gggaaactcg cccgtaggtg tgaagtgagg ggaaaaccgg tgataaagta aaaagcttac
    10741 ctaacactat agtaacaaag aaagcccaat tatcaatttt agtgctgagg aattggtctc
    10801 tttaataaat ttccttaacg ttgtaaatcc gcattttcct gacggtaccc c
//
