LOCUS       ISPa38                  6455 bp    DNA     linear       06-DEC-2020
DEFINITION  Citrobacter freundii.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Unclassified.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of ISPa38
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
     mobile_element  1..6455
                     /gene="ISPa38"
                     /product="Insertion sequence: ISPa38"
     repeat_region   1..39
                     /gene="IRL_ISPa38"
                     /product="ISPa38 inverted repeat left"
     CDS             complement(34..3000)
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISPa38 transposase"
                     /translation="MPRRSILSAAERESLLALPDTKDELIRHYTFSESDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGADEPPFPPLLRLVANQLKVGIESWDEYGQREQTRRE
                     HLVELQTVFGFQPFTIGHYRQAVQLLTELAMQTDKGIVLARALIEHLRRQSVIVPALN
                     AVERASAEAITRANRRLYDALAEPLTDVHRRRLDDLLKRRDNGKTTWLAWLRQSPVKP
                     NSRHMLEHIERLKAWQALDLPSGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALAIEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLFGRIG
                     QALIEAKQAGRDPFAAIEAVMSWDAFAESVTEAQRLAQPEDFDFLHRIGESYATLRRY
                     APEFLDVLKLRAAPAAKDVLDAIEVLRSMNSDNARKVPTDAPTEFIKPRWQKLVMTDT
                     GIDRRYYELCALSELKNALRSGDIWVQGSRQFKDFEDYLVPPAKFASLKQASELPLAV
                     ATDCDQYLHDRLTLLETQLPTVNRMALANELPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMILPHVKITELLLEVDEWTGFTRHFAHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHIRDETYGAALAELVNAQFRHPFAEHWGDGTTSSSDGQ
                     NFRTGSKAESTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGVRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDATYEALKP
                     MIGGTLNIKHVRAHWDEILRMATSIKQGTATASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAANALRGHGQAVDDGLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     CDS             3159..3449
                     /gene="orf291"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MRPSVVLDMKRSAVREAVGRFRAANPRVFGSVLHGTDRDGSDLD
                     LLVDALPGATLLDLGDLEEELKSLLGVDVDLLTPGDLPPKFRAKVLAEAQPI"
     CDS             3446..3832
                     /gene="orf387"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MSENRLPDYLDHIQQAATDARSFVEGMAKDDFLADKRTQQAVIM
                     SLIVIGEAATKVMDGYVEFTQAHADVPWRSMRNMRNRMAHGYFDINLDVVWETVQEWL
                     PALLQQLPAVRQDADDEDRNDKGMEP"
     CDS             4051..4425
                     /gene="orf375"
                     /codon_start=1
                     /transl_table=11
                     /product="Cupin 2 conserved barrel domain protein"
                     /translation="MKEGSPMTVESRIFSVAEYVQPSEGEPIRSVVLETRDSIIVVWH
                     VHPGQEIAAHIHPHGQDTWTVLSGMADYFQGNGIVRALREGEIAVARPGQVHGARNTG
                     TEPFVFVSVVASANAGFVLAER"
     CDS             4680..5006
                     /gene="orf327"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MSRSRRKTPIVGHTTCGSEREDKKLWHQRWRTRERTALTSASPE
                     ALSAHLPLLENQASSVWSMGKDGRSYWPVKRQAATADRIANHKGRNPQERASLKKRLL
                     RKWMSK"
     CDS             5003..5503
                     /gene="orf501"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MKLSFHQHIALFWMIGAPGVFAPVIENAKRPDAGAVMAWGVAIV
                     AVMILFTPLLLRCPPFRRWYGRTDALSERQRQALAERGLRRYYQTAFDDGYVPRVMPY
                     VWRIIWTVGGLMAVTAVLPTNTGRPAFDALVVFSTWYPIGVMLLVFASRPLGRLIRQR
                     AQERRK"
     CDS             5500..5871
                     /gene="orf372"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MSTSTIEALASAWARIAEEAEFPADYEGTATPQAHRASEAIQEQ
                     IRERIVATNDMRLFSLLHLLGQASLRMEQALWPEDYERMTREVEEALRQATDANARSY
                     THEEVMQAMQERIDRARDKPC"
     CDS             5865..6422
                     /gene="tnpR"
                     /codon_start=1
                     /transl_table=11
                     /product="ISPa38 resolvase"
                     /translation="MLIGYARVSTQDQNLELQREALSKAGCKKVFEDKVSGTRADRPG
                     LAKTLEMLREGDTLVVWKLDRLGRSVKQLVDLVGDLHKHGVQFRSLTDSIDTGTPSGR
                     FFFHVMASLAEMERELTVERTRAGLEVAKQLGRKGGRKPKMTDSKIESAKKLLASGVP
                     PKDVAKNLGVSIPTLYRWVPASTHA"
     repeat_region   6417..6455
                     /gene="IRR_ISPa38"
                     /product="ISPa38 inverted repeat right"
BASE COUNT     1164 a   1953 c   2125 g   1213 t
ORIGIN
        1 ggggtcgtct cagaaaacgg aaaataaagc acgctaagcc ggttgcagcg gccgtagcgg
       61 cctgaacttg cccgcgccga tcttggcgct gctgcgccag aggtaatcgc cggtcaggtt
      121 gatgtgctcc cagccgagcg gcgacaggta ctgcaacagg ccgtcatcga cggcttgacc
      181 gtggccacgc aaggcgttcg cggcccgctc cagatagacc gtgttccata gcacgatggc
      241 cgccgtcacc aggttgaggc cgctggcccg gtagcgctgc tgctcgaagc tgcggtcgcg
      301 gatttccccc aggcggttga agaacacggc gcgggccagc gcgttgcgcg cctcgccttt
      361 gttcagcccg gcatgcacgc ggcggcgcag ctcgacgctt tgcagccagt ccaggatgaa
      421 cagtgtgcgc tcgatgcgtc ccagctcacg cagggcgacg gccaggccgt tctggcgcgg
      481 gtagctgcca agcttcctga gcatcagcga ggccgtcgcc gtgccctgct tgatcgaggt
      541 ggccatccgc aggatttcat cccaatgggc gcggacgtgc ttgatgttga gcgtgccgcc
      601 gatcatcggt ttcaacgcct cgtaggtggc atcgcccttc gggatgtaga gcttggtgtc
      661 gcccaggtca cgaatgcgcg gggcgaagcg gaagcccagc aggtgcatca acgcgaagac
      721 gtggtccgtg aaccctgccg tgtcggtgta gtgctcctcg atccgcaggt cggattcgtg
      781 atacagcagg ccgtcgagca cgtaggtcga gtcgcgcacg ccgacgttca cgaccttggt
      841 gtggaacggc gcgtactggt cggagatatg ggtgtagaac gtccgccctg ggctgctgcc
      901 gtatttcgga ttgatgtggc cggtgctctc ggccttgctg ccggtgcgga agttctggcc
      961 gtccgacgat gacgtggtgc cgtcgcccca atgctcggcg aagggatgtc ggaactgcgc
     1021 gttgaccagc tcggccagtg ccgccccgta ggtttcgtcg cggatgtgcc aggcttgcag
     1081 ccaggccagc ttggcgtagg tcgtgccggg gcacgattcc gccatcttgg tcaggcccag
     1141 gttgatcgcg tcggcgagga tcgtggtcag caacaggttt ttgtccttgg ccaggtcgcc
     1201 tgacttcagg tgggcgaagt gccgggtgaa gcccgtccat tcgtctacct ccagcagcaa
     1261 ttcggtgatc ttgacgtgcg gtaggatcat cgccgtctgg tcgatcaggg cctgtgcggt
     1321 atcgggcacc gccgcatcga gcggcgtgat cttcaggccc gactccgtga tgatggcgtc
     1381 cggcagctcg ttggccagcg ccatgcggtt gacggtgggg agctgcgttt ccagcagcgt
     1441 cagccggtca tgcaggtact ggtcgcaatc ggtggccacg gccagcggca attcgctggc
     1501 ctgcttgagg ctggcgaatt tcgcgggcgg caccaggtag tcctcgaagt ccttgaactg
     1561 gcgcgagcct tgcacccaga tgtcgccgga gcgcagcgcg ttcttcagct ccgacagcgc
     1621 gcacagttcg tagtagcgcc ggtcgatgcc ggtgtcggtc atcaccagct tctgccagcg
     1681 cggcttgatg aactcggtcg gcgcgtcggt gggcaccttg cgggcgttgt cgctgttcat
     1741 gctgcgcagc acctcgatgg cgtcgagtac gtccttggcg gcgggcgcgg cccgcaactt
     1801 gagcacgtcg agaaattccg gcgcgtagcg gcgcagcgtg gcgtagctct cgccgatgcg
     1861 gtgcaggaaa tcgaagtcct cgggttgcgc gagccgctgc gcttcggtga cgctctcggc
     1921 gaaagcatcc caggacatga cggcctcgat ggcagcgaac ggatcgcggc ccgcttgctt
     1981 ggcctcgatc agcgcctggc cgatgcgccc gaacagccgc accttggcat tgatcgcctt
     2041 gccggatgcc tggaactgct gctgatgctt gttcttggcg gcattgaaca gcttgcccag
     2101 gatgcggtca tgcaggtcga tgatttcgtc ggtgacggtg gccatgccct cgatggcgag
     2161 cgccaccagg gtcgcgtaac gccgctgcgg ctcgaacttc gccaggtcgg cgggcgtcat
     2221 ctggccgccc tcgcgggcga tcttgagcag ccggttctgg tgaaccagcc gctcgatgcc
     2281 ggagggcagg tcgagcgcct gccacgcctt gaggcgttcg atgtgttcca gcatgtgccg
     2341 cgagttcggt ttgaccgggg attgccgcag ccaggccagc cacgtcgtct tgccgttgtc
     2401 gcggcgcttg agcagatcgt cgaggcgacg gcgatgcacg tccgtcagcg gctcagccaa
     2461 ggcgtcgtag agacgccggt tggcgcgggt aatcgcttcg gcgctcgccc gctcgacggc
     2521 gttgagggcg ggcacaatga ccgactgccg ccgcaggtgc tcgatcaagg ctctggccag
     2581 cacgatgccc ttgtcggttt gcatggccag ctcggtcagc aactggacag cctgccggta
     2641 gtggccaatc gtgaacggct ggaagccgaa caccgtttgc agctcgacca ggtgctcgcg
     2701 tcgggtctgc tcacgctgcc cgtactcgtc ccagctttcg atgccgacct tgagctggtt
     2761 ggcgaccagt ctcagcaatg gcgggaacgg tggctcatca gcgccaagga tgacgccggg
     2821 aaagcgcagg tagcagagct gcaccgcgaa gcccagccga ttggccgggc cgcgccgctg
     2881 ccggatgatg gagaggtcgc tttcgctgaa cgtgtagtga cggatcaact catccttggt
     2941 gtccggcaac gccagcaggc tttcgcgctc ggcggcggag aggatcgaac ggcggggcat
     3001 gcggtttcct tcttcttgaa aacgtaggtt tgtgacaagc ccgccaaggc aaccggcgcg
     3061 gcacgggaat caaggcattg cgattctcga aaatagttct tgaaattcta ttcttgattg
     3121 catatcatct caacgagttt cgataagaaa ggatgcccat gcgaccgtct gttgtgcttg
     3181 acatgaagcg aagcgcagtg cgtgaagcgg taggccgctt tcgcgccgcg aacccgcgcg
     3241 tcttcggctc ggtgctgcat ggcaccgacc gggatggcag cgacctcgac ctgttggtcg
     3301 atgcgctgcc cggtgccacg ttgttggact tgggcgattt ggaagaagaa ctgaaatcgc
     3361 tgctcggcgt tgacgtcgat ctgctgactc ccggcgacct gccgccgaag ttccgggcca
     3421 aggtgctcgc ggaggcgcaa ccgatatgag cgagaaccgc ctgcccgatt acctcgacca
     3481 cattcagcag gccgcaaccg atgcgcgcag cttcgtggaa gggatggcca aggacgactt
     3541 cttggccgac aagcgcaccc agcaggccgt catcatgagc ctgatcgtca tcggcgaggc
     3601 ggccacaaag gtgatggatg gctacgtcga gttcacccag gcgcatgccg acgtgccgtg
     3661 gcgcagcatg cgcaatatgc gtaatcgcat ggctcacggc tatttcgaca tcaacctcga
     3721 tgtggtgtgg gagacggtac aggaatggct gccggcgttg ctccagcaat tgcccgccgt
     3781 gcgtcaggat gccgacgatg aagaccgtaa cgacaaaggc atggagccat gaccaatcaa
     3841 gccgccgatg ttcggcccct ctggcgtgtt cgatcccgcg acctatgagc cgcgctcggg
     3901 caagaccttc tggatggccg caacggacgt gagttcgctg gtgggcaagg aggcagcagc
     3961 cgacacgctc atcggcaact gcacgaccgc acgaccagcc tcccgttcaa attcgagtta
     4021 gaactcatat ccagcctgtc tgggggcact gtgaaagagg gatctccgat gactgttgaa
     4081 tcgagaatat tttctgtagc cgagtatgtt cagccgtccg aaggcgagcc tattcgttcc
     4141 gttgtgcttg aaacccgaga ctcaattatc gtggtttggc atgtccatcc cgggcaggaa
     4201 attgcggctc acattcatcc tcacggccaa gacacgtgga ctgttttgtc gggaatggct
     4261 gattactttc agggcaatgg gattgttcgt gccctcaggg aaggtgagat agccgtggca
     4321 agaccgggcc aagtgcacgg ggcgcgaaat acaggtaccg agccatttgt gttcgtctcg
     4381 gttgtggcat cagccaatgc cggtttcgta ttggctgagc gatagagccc aatctctgga
     4441 gttggtccaa tgagcggtcg gggagatagg taacagacat gcagcggaca cggctgctaa
     4501 accaggtcgc aaacctcctt gcgtcgcagc gtgccgcaag cgacgcgatc aatcgaatgg
     4561 ggtcggcatg agactgaaca ccatccagtt cccgaccgcg tagccgcctg tcctgccgat
     4621 caggtcttga ccatcgacgc ctgggatcag tcctgaatgt tcttggagac cactacgtta
     4681 tgagccgcag ccgccgcaaa acacccatcg tcgggcacac gacctgcggc agcgagcgcg
     4741 aggacaagaa gctctggcat cagcgctggc gcacccgtga gcgcacggcg ctgaccagcg
     4801 cgtcgcccga agccctgagc gcccatctgc ccctgctgga aaaccaggcc agcagcgtct
     4861 ggtcgatggg caaggatggc cgctcctact ggcccgtcaa gcgccaggcc gccacggcgg
     4921 atcgcatcgc caatcacaag ggacgcaacc cgcaagaacg cgcctccctg aaaaagcgcc
     4981 tgctgcgcaa gtggatgagc aaatgaagct ctccttccat cagcacattg cgctgttctg
     5041 gatgatcggt gctccgggcg tcttcgcgcc cgtgatcgag aacgccaagc ggcccgatgc
     5101 cggcgccgtc atggcgtggg gtgtcgcgat cgtggcggtg atgatcctct tcaccccttt
     5161 gctgctgcgc tgtccaccat tccggcgctg gtatggccgg acggatgcgc tgtcggagcg
     5221 gcagcgccag gcgcttgccg agcgcggcct gcgccgctac taccagaccg ctttcgatga
     5281 cggctacgtg ccccgcgtga tgccctacgt gtggcgcatc atctggacgg tcggcgggtt
     5341 gatggctgtg accgccgtac tgcctaccaa caccggacgg ccagccttcg atgccttggt
     5401 ggtcttctcg acctggtatc cgataggcgt gatgctgctg gttttcgcgt cgaggcccct
     5461 tggccggttg attcgccaaa gagcacagga gcggcggaaa tgagcacgtc aaccatcgag
     5521 gcgctggcca gcgcctgggc aaggattgcc gaggaagcgg aattccccgc tgactacgag
     5581 gggactgcca caccacaagc gcatcgggct agcgaagcta ttcaggagca gattcgggag
     5641 cgcatcgtcg ccaccaacga catgcggctg ttcagcctgc tgcacctgct gggtcaggcg
     5701 tcgctgcgca tggagcaagc gctgtggccg gaggattacg agcggatgac gcgcgaggtt
     5761 gaggaagccc tgcggcaagc caccgacgcc aacgccagat cgtacaccca cgaagaagtg
     5821 atgcaggcga tgcaggaacg catcgaccgg gcgcgagaca agccatgttg attggctatg
     5881 cgcgcgtctc gacgcaggat cagaacctgg agctgcaacg cgaagccttg agcaaggccg
     5941 gatgtaaaaa ggtcttcgag gacaaggtga gtggcacgcg ggcagaccgg cctggcttgg
     6001 ccaagacgct cgaaatgctg cgcgaaggcg atactttggt cgtctggaag ctcgaccggc
     6061 tgggccggtc ggtcaagcaa ctggtcgatc tggtcggcga tctgcacaag cacggtgtcc
     6121 agttcaggag cctcaccgac tccatcgaca ccggcacacc atccgggcgg ttcttcttcc
     6181 acgtcatggc gagccttgcc gaaatggagc gcgagctgac cgtcgagcgc acccgcgccg
     6241 ggctggaagt cgccaagcag ctcggccgca aaggcggccg caagccgaag atgaccgaca
     6301 gcaagatcga gtcggccaag aagctgctgg ccagcggggt gccgcccaag gacgtggcca
     6361 agaacctcgg cgtgtccatt ccgacgctgt accgctgggt gccagcctcc acgcacgctt
     6421 agcgtgcttt attttccgtt ttctgagacg acccc
//
