LOCUS       In4                     8330 bp    DNA     linear   BCT 29-SEP-2020
DEFINITION  Escherichia coli.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Escherichia.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of In4
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     repeat_region   1..24
                     /gene="IRi_In4"
     CDS             complement(203..1216)
                     /gene="intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="Integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVNWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPTLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     misc_recomb     1297..1378
                     /gene="attI1"
     CDS             1318..1851
                     /gene="aacC1e"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3-N-acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAAL
                     DLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQP
                     RSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKL
                     GIREEVMHFDIDPSTAT"
     misc_recomb     1846..1954
                     /gene="attC_aacC1e"
     CDS             1969..2214
                     /gene="gcuE"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MRAATYFSAPPCSKGFLAFWASSACAASATSYPSCSAAPLSWSS
                     GFSWAAPFFKAGRSLLAFGSNSAVKRTRILRAAYLGR"
     misc_recomb     2157..2216
                     /gene="attC_gcuE"
     CDS             2232..3011
                     /gene="aadA2"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside-3''-adenylyltransferase"
                     /translation="MTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKPYSDI
                     DLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYPAKRE
                     LQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQDLFE
                     ALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPAQYQP
                     VLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
     misc_recomb     3013..3072
                     /gene="attC_aadA2"
     CDS             3273..4532
                     /gene="cmlA1"
                     /codon_start=1
                     /transl_table=11
                     /product="Chloramphenicol and florfenicol resistance
                     protein"
                     /translation="MSSKNFSWRYSLAATVLLLSPFDLLASLGMDMYLPAVPFMPNAL
                     GTTASTIQLTLTTYLVMIGAGQLLFGPLSDRLGRRPVLLGGGLAYVVASMGLALTSSA
                     EVFLGLRILQACGASACLVSTFATVRDIYAGREESNVIYGILGSMLAMVPAVGPLLGA
                     LVDMWLGWRAIFAFLGLGMIAASAAAWRFWPETRVQRVAGLQWSQLLLPVKCLNFWLY
                     TLCYAAGMGSFFVFFSIAPGLMMGRQGVSQLGFSLLFATVAIAMVFTARFMGRVIPKW
                     GSPSVLRMGMGCLIAGAVLLAITEIWALQSVLGFIAPMWLVGIGVATAVSVAPNGALR
                     GFDHVAGTVTAVYFCLGGVLLGSIGTLIISLLPRNTAWPVVVYCLTLATVVLGLSCVS
                     RVKGSRGQGEHDVVALQSAESTSNPNR"
     misc_recomb     4552..4621
                     /gene="attC_cmlA1"
     CDS             4724..5071
                     /gene="qacED1"
                     /codon_start=1
                     /transl_table=11
                     /product="Quaternary ammonium compound resistance protein"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     CDS             5065..5904
                     /gene="sul1"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydropteroate synthase"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     CDS             6032..6532
                     /gene="orf5"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     CDS             6556..6843
                     /gene="orf6"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MSGWDGPVASRSLAVRPRCLGCARGCRYPPLPSSTLDNAAAVIA
                     ADARAGLFGPVRLARPSACLGEISGGRINGGASPPFVRVKAEDSSLKNIRL"
     repeat_region   complement(6855..6879)
                     /gene="IRt_In4"
     repeat_region   6978..6991
                     /gene="IRL_IS6100"
     CDS             7039..7803
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6100 transposase"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     repeat_region   7844..7857
                     /gene="IRR_IS6100"
     misc_feature    7858..8124
                     /gene="truncated tnpA"
                     /product="Truncated IS6100 transposase (pseudogene)"
     repeat_region   8165..8178
                     /gene="IRR_IS6100"
     repeat_region   8306..8330
                     /gene="IRt_In4"
ORIGIN      
        1 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
       61 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
      121 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
      181 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
      241 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
      301 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
      361 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
      421 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
      481 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
      541 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
      601 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcagcgtgg
      661 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
      721 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
      781 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
      841 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
      901 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
      961 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     1021 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     1081 ggatgaaggc acgaacccag ttgacataag cctgttcggt tcgtaaactg taatgcaagt
     1141 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1201 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat
     1261 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1321 ttacgcagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaggtggc
     1381 tcaagtatgg gcatcattcg cacatgtagg ctcggccctg accaagtcaa atccatgcgg
     1441 gctgctcttg atcttttcgg tcgtgagttc ggagacgtag ccacctactc ccaacatcag
     1501 ccggactccg attacctcgg gaacttgctc cgtagtaaga cattcatcgc gcttgctgcc
     1561 ttcgaccaag aagcggttgt tggcgctctc gcggcttacg ttctgcccag gtttgagcag
     1621 ccgcgtagtg agatctatat ctatgatctc gcagtctccg gcgagcaccg gaggcagggc
     1681 attgccaccg cgctcatcaa tctcctcaag catgaggcca acgcgcttgg tgcttatgtg
     1741 atctacgtgc aagcagatta cggtgacgat cccgcagtgg ctctctatac aaagttgggc
     1801 atacgggaag aagtgatgca ctttgatatc gacccaagta ccgccaccta acaattcgtt
     1861 caagccgaga tcggcttccc ggccgcggag ttgttcggta aattgtcaca acgccgcggc
     1921 cgcaaagcgc tccggcttaa ctcaggcgtt agtcccatca cccgctttat gcgcgcagcc
     1981 acttatttca gcgctccgcc ctgttccaaa ggctttctag ccttttgggc ctccagcgct
     2041 tgtgcagcaa gcgccaccag ctatccttct tgcagcgctg cgccgctttc gtggtccagc
     2101 ggtttttctt gggctgcgcc cttcttcaag gccgggcgct cccttttggc cttcgggtct
     2161 aactctgcgg tcaagcggac ccgcattctg cgggccgctt accttggccg ttagacatca
     2221 tgagggtagc ggtgaccatc gaaatttcga accaactatc agaggtgcta agcgtcattg
     2281 agcgccatct ggaatcaacg ttgctggccg tgcatttgta cggctccgca gtggatggcg
     2341 gcctgaagcc atacagcgat attgatttgt tggttactgt ggccgtaaag cttgatgaaa
     2401 cgacgcggcg agcattgctc aatgacctta tggaggcttc ggctttccct ggcgagagcg
     2461 agacgctccg cgctatagaa gtcacccttg tcgtgcatga cgacatcatc ccgtggcgtt
     2521 atccggctaa gcgcgagctg caatttggag aatggcagcg caatgacatt cttgcgggta
     2581 tcttcgagcc agccatgatc gacattgatc tagctatcct gcttacaaaa gcaagagaac
     2641 atagcgttgc cttggtaggt ccggcagcgg aggaattctt tgacccggtt cctgaacagg
     2701 atctattcga ggcgctgagg gaaaccttga agctatggaa ctcgcagccc gactgggccg
     2761 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca ataaccggca
     2821 aaatcgcgcc gaaggatgtc gctgccgact gggcaataaa acgcctacct gcccagtatc
     2881 agcccgtctt acttgaagct aagcaagctt atctgggaca aaaagaagat cacttggcct
     2941 cacgcgcaga tcacttggaa gaatttattc gctttgtgaa aggcgagatc atcaagtcag
     3001 ttggtaaatg atgtctaaca attcgttcaa gccgaccgcg ctacgcgcgg cggcttaact
     3061 ccggcgttgg gcgcacaata aggctccttg cagagttgct tgaaagttgt tacgattcaa
     3121 attcaatcat gagatagtca gcagatgagc acttccaaga acgcagacaa gtaagccgca
     3181 gcaaccttca tttttcggtt gttgcggcgt tctcatgaat ccttttgctc tacgggagcg
     3241 ccgccaaatc ctttgttcaa ggagatggtt tcgtgagctc aaaaaacttt agttggcggt
     3301 actcccttgc cgccacggtg ttgttgttat caccgttcga tttattggca tcactcggca
     3361 tggacatgta cttgccagca gtgccgttta tgccaaacgc gcttggtacg acagcgagca
     3421 caattcagct tacgctgaca acgtacttgg tcatgattgg tgccggtcag ctcttgtttg
     3481 gaccgctatc ggaccgactg gggcgccgcc ccgttctact gggaggtggc ctcgcctacg
     3541 ttgtggcgtc aatgggcctc gctcttacgt catcggctga agtctttctg gggcttcgga
     3601 ttcttcaggc ttgtggtgcc tcggcgtgcc ttgtttccac atttgcaaca gtacgtgaca
     3661 tttacgcagg tcgcgaggaa agtaatgtca tttacggcat actcggatcc atgctggcca
     3721 tggtcccggc ggtaggccca ttgctcggag cgctcgtcga catgtggctt gggtggcggg
     3781 ctatctttgc gtttctaggt ttgggcatga tcgctgcatc tgcagcagcg tggcgattct
     3841 ggcctgaaac ccgggtgcaa cgagttgcgg gcttgcaatg gtcgcagctg ctactccccg
     3901 ttaagtgcct gaacttctgg ttgtacacgt tgtgttacgc cgctggaatg ggtagcttct
     3961 tcgtcttttt ctccattgcg cccggactaa tgatgggcag gcaaggtgtg tctcagcttg
     4021 gcttcagcct gctgttcgcc acagtggcaa ttgccatggt gtttacggct cgttttatgg
     4081 ggcgtgtgat acccaagtgg ggcagcccaa gtgtcttgcg aatgggaatg ggatgcctga
     4141 tagctggagc agtattgctt gccatcaccg aaatatgggc tttgcagtcc gtgttaggct
     4201 ttattgctcc aatgtggcta gtgggtattg gtgtcgccac agcggtatct gtggcgccca
     4261 atggcgctct tcgaggattc gaccatgttg ctggaacggt cacggcagtc tacttctgct
     4321 tgggcggtgt actgctagga agcatcggaa cgttgatcat ttcgctgttg ccgcgcaaca
     4381 cggcttggcc ggttgtcgtg tactgtttga cccttgcaac agtcgtgctc ggtctgtctt
     4441 gtgtttcccg agtgaagggc tctcgcggcc agggggagca tgatgtggtc gcgctacaaa
     4501 gtgcggaaag tacatcaaat cccaatcgtt gagagaatgt ggcaagctat cgcccaacaa
     4561 atcgctgcag ccgacccaaa accgctacgc ggtttcggtc ggctgagctc aggcgttaga
     4621 tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct tttattattt
     4681 ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgaaag gctggctttt
     4741 tcttgttatc gcaatagttg gcgaagtaat cgcaacatcc gcattaaaat ctagcgaggg
     4801 ctttactaag cttgcccctt ccgccgttgt cataatcggt tatggcatcg cattttattt
     4861 tctttctctg gttctgaaat ccatccctgt cggtgttgct tatgcagtct ggtcgggact
     4921 cggcgtcgtc ataattacag ccattgcctg gttgcttcat gggcaaaagc ttgatgcgtg
     4981 gggctttgta ggtatggggc tcataattgc tgcctttttg ctcgcccgat ccccatcgtg
     5041 gaagtcgctg cggaggccga cgccatggtg acggtgttcg gcattctgaa tctcaccgag
     5101 gactccttct tcgatgagag ccggcggcta gaccccgccg gcgctgtcac cgcggcgatc
     5161 gaaatgctgc gagtcggatc agacgtcgtg gatgtcggac cggccgccag ccatccggac
     5221 gcgaggcctg tatcgccggc cgatgagatc agacgtattg cgccgctctt agacgccctg
     5281 tccgatcaga tgcaccgtgt ttcaatcgac agcttccaac cggaaaccca gcgctatgcg
     5341 ctcaagcgcg gcgtgggcta cctgaacgat atccaaggat ttcctgaccc tgcgctctat
     5401 cccgatattg ctgaggcgga ctgcaggctg gtggttatgc actcagcgca gcgggatggc
     5461 atcgccaccc gcaccggtca ccttcgaccc gaagacgcgc tcgacgagat tgtgcggttc
     5521 ttcgaggcgc gggtttccgc cttgcgacgg agcggggtcg ctgccgaccg gctcatcctc
     5581 gatccgggga tgggattttt cttgagcccc gcaccggaaa catcgctgca cgtgctgtcg
     5641 aaccttcaaa agctgaagtc ggcgttgggg cttccgctat tggtctcggt gtcgcggaaa
     5701 tccttcttgg gcgccaccgt tggccttcct gtaaaggatc tgggtccagc gagccttgcg
     5761 gcggaacttc acgcgatcgg caatggcgct gactacgtcc gcacccacgc gcctggagat
     5821 ctgcgaagcg caatcacctt ctcggaaacc ctcgcgaaat ttcgcagtcg cgacgccaga
     5881 gaccgagggt tagatcatgc ctagcattca ccttccggcc gcccgctagc ggaccctggt
     5941 caggttccgc gaaggtgggc gcagacatgc tgggctcgtc aggatcaaac tgcactatga
     6001 ggcggcggtt cataccgcgc caggggagcg aatggacagc gaggagcctc cgaacgttcg
     6061 ggtcgcctgc tcgggtgata tcgacgaggt tgtgcggctg atgcacgacg ctgcggcgtg
     6121 gatgtccgcc aagggaacgc ccgcctggga cgtcgcgcgg atcgaccgga cattcgcgga
     6181 gaccttcgtc ctgagatccg agctcctagt cgcgagttgc agcgacggca tcgtcggctg
     6241 ttgcaccttg tcggccgagg atcccgagtt ctggcccgac gccctcaagg gggaggccgc
     6301 atatctgcac aagctcgcgg tgcgacggac acatgcgggc cggggtgtca gctccgcgct
     6361 gatcgaggct tgccgccatg ccgcgcgaac gcaggggtgc gccaagctgc ggctcgactg
     6421 ccacccgaac ctgcgtggcc tatacgagcg gctcggattc acccacgtcg acactttcaa
     6481 tcccggctgg gatccaacct tcatcgcaga acgcctagaa ctcgaaatct aacgtccgtt
     6541 cgggcatcga ggtccatgtc ggggtgggac gggcccgtgg cttcaagatc acttgcagtc
     6601 cgaccgcgat gtcttggttg cgcgagaggt tgtcgatatc ctccacttcc atcatcaacc
     6661 ctggataatg ccgccgccgt catcgccgcc gacgcccgtg ccgggctttt cgggcctgtc
     6721 aggcttgctc ggccttcagc ctgcctgggc gagatctccg gcggacggat taacggcgga
     6781 gcttcgccgc ctttcgtgcg tgtgaaggcc gaagatagtt ctctcaaaaa catccgttta
     6841 tgagagatac caaatgtcat tttcagaaga cgactgcacc agttgattgg gcgtaatggc
     6901 tgttgtgcag ccagctcctg acagttcaat atcagaagtg atctgcacca atctcgacta
     6961 tgctcaatac tcgtgtgggc tctgttgcaa aaatcgtgaa gcttgagcat gcttggcgga
     7021 gattggacgg acggaacgat gacggatttc aagtggcgcc atttccaggg tgatgtgatc
     7081 ctgtgggcgg tgcgctggta ttgtcgctat ccgatcagct atcgcgacct tgaggaaatg
     7141 ctggcggaac gcggcatttc ggtcgaccat acgacgatct atcgctgggt ccagtgctac
     7201 gccccggaga tggagaagcg gctgcgctgg ttctggcggc gtggctttga tccgagctgg
     7261 cgcctggatg aaacctacgt caaggtgcgg ggcaagtgga cctacctgta ccgggcagtc
     7321 gacaagcggg gcgacacgat cgatttctac ctgtcgccga cccgcagcgc caaggcagcg
     7381 aagcggttcc tgggcaaggc cctgcgaggc ctgaagcact gggaaaagcc tgccacgctc
     7441 aataccgaca aagcgccgag ctatggtgca gcgatcaccg aattgaagcg cgaaggaaag
     7501 ctggaccggg agacggccca ccggcaggtg aagtatctca ataacgtgat cgaggccgat
     7561 cacggaaagc tcaagatact gatcaagccg gtgcgcggtt tcaaatcgat ccccacggcc
     7621 tatgccacga tcaagggatt cgaagtcatg cgagccctgc gcaaaggaca ggctcgcccc
     7681 tggtgcctgc agcccggcat caggggcgag gtgcgccttg tggagagagc ttttggcatt
     7741 gggccctcgg cgctgacgga ggccatgggc atgctcaacc accatttcgc agcagccgcc
     7801 tgatcggcgc agagcgacag cctacctctg actgccgcca atctttgcaa cagagccctc
     7861 aataacgtga tcgaggccga tcacggaaag ctcaagatac tgatcaagcc ggtgcgcggt
     7921 ttcaaatcga tccccacggc ctatgccacg atcaagggat tcgaagtcat gcgagccctg
     7981 cgcaaaggac aggctcgccc ctggtgcctg cagcccggca tcaggggcga ggtgcgcctt
     8041 gtggagagag cttttggcat tgggccctcg gcgctgacgg aggccatggg catgctcaac
     8101 caccatttcg cagcagccgc ctgatcggcg cagagcgaca gcctacctct gactgccgcc
     8161 aatctttgca acagagcctc cgtcgccatg ctcacctcgc tttggtgcac acgagtattg
     8221 agcatagtcg agattggtgc agatcacttc tgatattgaa ctgtcaggag ctggctgcac
     8281 aacagccatt acgcccaatc aactggtgca gtcgtcttct gaaaatgaca
//
