LOCUS       In37                   13188 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  Escherichia coli.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia; Escherichia
            coli.
REFERENCE   1  (bases 1 to 13188)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of In37
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13188)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
     mobile_element  1..13188
                     /gene="In37"
                     /product="Complex class 1 integron: In37"
     repeat_region   1..25
                     /gene="IRi_In37"
                     /product="Inverted repeat at the integrase end of In37"
     CDS             complement(203..1216)
                     /gene="intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="Integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     regulatory      1101..1129
                     /gene="PcH1 (Hybrid 1)"
                     /product="Promoter PcH1 (Hybrid 1)"
     regulatory      1101..1106
                     /gene="-35 region_PcH1 (Hybrid 1)"
                     /product="-35 region of PcH1 (Hybrid 1)"
     regulatory      1124..1129
                     /gene="-10 region_PcH1 (Hybrid 1)"
                     /product="-10 region of PcH1 (Hybrid 1)"
     misc_recomb     1297..1359
                     /gene="attI1"
                     /product="attI1 site"
     CDS             1463..2062
                     /gene="aacA4cr"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside N(6')-acetyltransferase type 1"
                     /translation="MSNAKTKLGITKYSIVTNSNDSVTLRLMTEHDLAMLYEWLNRSH
                     IVEWWGGEEARPTLADVQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGR
                     WEEETDPGVRGIDQLLANASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRA
                     IRCYEKAGFERQGTVTTPYGPAVYMVQTRQAFERTRSDA"
     misc_recomb     2057..2128
                     /gene="attC_aacA4cr"
                     /product="attC site for aacA4cr"
     CDS             2193..3023
                     /gene="blaOXA-1"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase OXA-1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     misc_recomb     3043..3132
                     /gene="attC_blaOXA-1"
                     /product="attC site for blaOXA-1"
     CDS             3161..3793
                     /gene="catB3"
                     /codon_start=1
                     /transl_table=11
                     /product="Chloramphenicol acetyltransferase"
                     /translation="MTNYFDSPFKGKLLSEQVKNPNIKVGRYSYYSGYYHGHSFDDCA
                     RYLFPDRDDVDKLIIGSFCSIGSGASFIMAGNQGHRYDWASSFPFFYMQEEPAFSSAL
                     DAFQKAGNTVIGNDVWIGSEAMVMPGIKIGHGAVIGSRSLVTKDVEPYAIVGGNPAKK
                     IKKRFTDEEISLLLEMEWWNWSLEKIKAAMPMLCSSNIVGLHKYWLEFAV"
     misc_recomb     3788..3847
                     /gene="attC_catB3"
                     /product="attC site for catB3"
     CDS             3878..4330
                     /gene="arr3"
                     /codon_start=1
                     /transl_table=11
                     /product="Rifampin ADP-ribosyl transferase"
                     /translation="MVKDWIPISHDNYKQVQGPFYHGTKANLAIGDLLTTGFISHFED
                     GRILKHIYFSALMEPAVWGAELAMSLSGLEGRGYIYIVEPTGPFEDDPNLTNKRFPGN
                     PTQSYRTCEPLRIVGVVEDWEGHPVELIRGMLDSLEDLKRRGLHVIED"
     misc_recomb     4337..4450
                     /gene="attC_arr3"
                     /product="attC site for arr3"
     CDS             4553..4900
                     /gene="qacED1"
                     /codon_start=1
                     /transl_table=11
                     /product="Quaternary ammonium compound resistance protein"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     CDS             4894..5733
                     /gene="sul1"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydropteroate synthase"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     mobile_element  5758..9809
                     /gene="Truncated ISCR1-qnrA1 unit"
                     /product="Putative resistance unit: truncated ISCR1-qnrA1
                     unit"
     mobile_element  complement(5758..7911)
                     /gene="ISCR1"
                     /product="Insertion sequence: ISCR1"
     CDS             6138..7679
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCR1 transposase"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASL
                     EAQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGAR
                     RMAESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIK
                     KAGYTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYD
                     ELNTLAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGP
                     QQGRKVFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPA
                     VSEKRLALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVF
                     APNSKHRVQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVC
                     EHCGGHVKVIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQ
                     SRLFD"
     repeat_region   7893..7911
                     /gene="oriIS"
                     /product="ISCR1 oriIS"
     CDS             7936..8592
                     /gene="qnrA1"
                     /codon_start=1
                     /transl_table=11
                     /product="Quinolone resistance protein"
                     /translation="MDIIDKVFQQEDFSRQDLSDSRFRRCRFYQCDFSHCQLQDASFE
                     DCSFIESGAVEGCHFSYADLRDASFKACRLSLANFSGANCFGIEFRECDLKGANFSRA
                     RFYNQVSHKMYFCSAYISGCNLAYTNLSGQCLEKCELFENNWSNANLSGASLMGSDLS
                     RGTFSRDCWQQVNLRGCDLTFADLDGLDPRRVNLEGVKICAWQQEQLLEPLGVIVLPD
                     "
     CDS             8778..9653
                     /gene="ampR"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR-family regulator"
                     /translation="MVRRYLPLNPLRAFEAAARHLSFTRAAIELNVTHAAVSQQVRAL
                     EEQLGCVLFTRVSRGLVLTHEGEGLLPVLNEAFDRIADTLECFSHGQFRERVKVGAVG
                     TFAAGWLLPRLAGFYDSHPHIDLHISTHNNHVDPAAEGHDYTIRFGNGAWHESDAELI
                     FSAPHAPLCSPAIAEQLQQPDDVHRFTLLRSFRRDEWSRWLDCAGGTPPSPSQPVMVF
                     DTSLAMAEAAQLGAGVAIAPVCMFSRLLQSGALVQPFAAEITLGGYWLTRLQSRTETP
                     AMQQFARWLLNTAAA"
     misc_feature    complement(9677..9809)
                     /gene="truncated hypA"
                     /product="Hydrogenase nickel incorporation protein HypA
                     (pseudogene)"
     misc_feature    9810..10250
                     /gene="truncated qacED1"
                     /product="Truncated quaternary ammonium compound
                     resistance protein"
     CDS             10244..11083
                     /gene="sul1"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydropteroate synthase"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     CDS             11211..11711
                     /gene="orf5"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     CDS             11735..12022
                     /gene="orf6"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MSGWDGPVASRSLAVRPRCLGCARGCRYPPLPSSTLDNAAAVIA
                     ADARAGLFGPVRLARPSACLGEISGGRINGGASPPFVRVKAEDSSLKNIRL"
     repeat_region   complement(12034..12058)
                     /gene="IRt_In37"
                     /product="Inverted repeat at the tni end of In37"
     mobile_element  12157..13036
                     /gene="IS6100"
                     /product="Insertion sequence: IS6100"
     repeat_region   12157..12170
                     /gene="IRL_IS6100"
                     /product="IS6100 inverted repeat left"
     CDS             12218..12982
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6100 transposase"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     repeat_region   13023..13036
                     /gene="IRR_IS6100"
                     /product="IS6100 inverted repeat right"
     repeat_region   13164..13188
                     /gene="IRt_In37"
                     /product="Inverted repeat at the tni end of In37"
BASE COUNT     3046 a   3637 c   3578 g   2927 t
ORIGIN
        1 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
       61 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
      121 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
      181 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
      241 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
      301 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
      361 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
      421 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
      481 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
      541 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
      601 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
      661 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
      721 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
      781 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
      841 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
      901 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
      961 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     1021 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     1081 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaaactg taatgcaagt
     1141 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1201 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat
     1261 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1321 ttacgcagca gggcagtcgc cctaaaacaa agttagatgc actaagcaca taattgctca
     1381 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta
     1441 cacaaattgg gagttagaca tcatgagcaa cgcaaaaaca aagttaggca tcacaaagta
     1501 cagcatcgtg accaacagca acgattccgt cacactgcgc ctcatgactg agcatgacct
     1561 tgcgatgctc tatgagtggc taaatcgatc tcatatcgtc gagtggtggg gcggagaaga
     1621 agcacgcccg acacttgctg acgtacagga acagtacttg ccaagcgttt tagcgcaaga
     1681 gtccgtcact ccatacattg caatgctgaa tggagagccg attgggtatg cccagtcgta
     1741 cgttgctctt ggaagcgggg acggacggtg ggaagaagaa accgatccag gagtacgcgg
     1801 aatagaccag ttactggcga atgcatcaca actgggcaaa ggcttgggaa ccaagctggt
     1861 tcgagctctg gttgagttgc tgttcaatga tcccgaggtc accaagatcc aaacggaccc
     1921 gtcgccgagc aacttgcgag cgatccgatg ctacgagaaa gcggggtttg agaggcaagg
     1981 taccgtaacc accccatatg gtccagccgt gtacatggtt caaacacgcc aggcattcga
     2041 gcgaacacgc agtgatgcct aacccttcca tcgaggggga cgtccaaggg ctggcgccct
     2101 tggccgcccc tcatgtcaaa cgttgggcga acccggagcc tcattaattg ttagccgtta
     2161 aaattaagcc ctttaccaaa ccaatactta ttatgaaaaa cacaatacat atcaacttcg
     2221 ctattttttt aataattgca aatattatct acagcagcgc cagtgcatca acagatatct
     2281 ctactgttgc atctccatta tttgaaggaa ctgaaggttg ttttttactt tacgatgcat
     2341 ccacaaacgc tgaaattgct caattcaata aagcaaagtg tgcaacgcaa atggcaccag
     2401 attcaacttt caagatcgca ttatcactta tggcatttga tgcggaaata atagatcaga
     2461 aaaccatatt caaatgggat aaaaccccca aaggaatgga gatctggaac agcaatcata
     2521 caccaaagac gtggatgcaa ttttctgttg tttgggtttc gcaagaaata acccaaaaaa
     2581 ttggattaaa taaaatcaag aattatctca aagattttga ttatggaaat caagacttct
     2641 ctggagataa agaaagaaac aacggattaa cagaagcatg gctcgaaagt agcttaaaaa
     2701 tttcaccaga agaacaaatt caattcctgc gtaaaattat taatcacaat ctcccagtta
     2761 aaaactcagc catagaaaac accatagaga acatgtatct acaagatctg gataatagta
     2821 caaaactgta tgggaaaact ggtgcaggat tcacagcaaa tagaacctta caaaacggat
     2881 ggtttgaagg gtttattata agcaaatcag gacataaata tgtttttgtg tccgcactta
     2941 caggaaactt ggggtcgaat ttaacatcaa gcataaaagc caagaaaaat gcgatcacca
     3001 ttctaaacac actaaattta taaaaaatct aatggcaaaa tcgcccaacc cttcaatcaa
     3061 gtcgggacgg ccaaaagcaa gcttttggct cccctcgctg gcgctcggcg ccccttattt
     3121 caaacgttag acggcaaagt cacagaccgc gggatctctt atgaccaact actttgatag
     3181 ccccttcaaa ggcaagctgc tttctgagca agtgaagaac cccaatatca aagttgggcg
     3241 gtacagctat tactctggct actatcatgg gcactcattc gatgactgcg cacggtatct
     3301 gtttccggac cgtgatgacg ttgataagtt gatcatcggt agtttctgct ctatcgggag
     3361 tggggcttcc tttatcatgg ctggcaatca ggggcatcgg tacgactggg catcatcttt
     3421 cccgttcttt tatatgcagg aagaacctgc attctcaagc gcactcgatg ccttccaaaa
     3481 agcaggtaat actgtcattg gcaatgacgt ttggatcggc tctgaggcaa tggtcatgcc
     3541 cggaatcaag atcgggcacg gtgcggtgat aggcagccgc tcgttggtga caaaagatgt
     3601 ggagccttac gctatcgttg gcggcaatcc cgctaagaag attaagaaac gcttcaccga
     3661 tgaggaaatt tcattgcttc tggagatgga gtggtggaat tggtcactgg agaagatcaa
     3721 agcggcaatg cccatgctgt gctcgtctaa tattgttggc ctgcacaagt attggctcga
     3781 gtttgccgtc taacaattca atcaagccga tgccgcttcg cggcacggct tatttcaggc
     3841 gttatgcagc caaatcccaa caattaaggg tcttaaaatg gtaaaagatt ggattcccat
     3901 ctctcatgat aattacaagc aggtgcaagg accgttctat catggaacca aagccaattt
     3961 ggcgattggt gacttgctaa ccacagggtt catctctcat ttcgaggacg gtcgtattct
     4021 taagcacatc tacttttcag ccttgatgga gccagcagtt tggggagctg aacttgctat
     4081 gtcactgtct ggcctcgagg gtcgcggcta catatacata gttgagccaa caggaccgtt
     4141 cgaagacgat ccgaatctta cgaacaaaag atttcccggt aatccaacac agtcctatag
     4201 aacctgcgaa cccttgagaa ttgttggcgt tgttgaagac tgggaggggc atcctgttga
     4261 attaataagg ggaatgttgg attcgttgga ggacttaaag cgccgtggtt tacacgtcat
     4321 tgaagactag tcctttgcat aacaaagcca tcaaaccgga cgccagagat tccgcgcctg
     4381 ttgcgcatgg cttcgccatt ttatgcgcaa taggcgcgcc accctgtcgc cgtttatggc
     4441 ggcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     4501 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg
     4561 ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatccg cattaaaatc
     4621 tagcgagggc tttactaagc ttgccccttc cgccgttgtc ataatcggtt atggcatcgc
     4681 attttatttt ctttctctgg ttctgaaatc catccctgtc ggtgttgctt atgcagtctg
     4741 gtcgggactc ggcgtcgtca taattacagc cattgcctgg ttgcttcatg ggcaaaagct
     4801 tgatgcgtgg ggctttgtag gtatggggct cataattgct gcctttttgc tcgcccgatc
     4861 cccatcgtgg aagtcgctgc ggaggccgac gccatggtga cggtgttcgg cattctgaat
     4921 ctcaccgagg actccttctt cgatgagagc cggcggctag accccgccgg cgctgtcacc
     4981 gcggcgatcg aaatgctgcg agtcggatca gacgtcgtgg atgtcggacc ggccgccagc
     5041 catccggacg cgaggcctgt atcgccggcc gatgagatca gacgtattgc gccgctctta
     5101 gacgccctgt ccgatcagat gcaccgtgtt tcaatcgaca gcttccaacc ggaaacccag
     5161 cgctatgcgc tcaagcgcgg cgtgggctac ctgaacgata tccaaggatt tcctgaccct
     5221 gcgctctatc ccgatattgc tgaggcggac tgcaggctgg tggttatgca ctcagcgcag
     5281 cgggatggca tcgccacccg caccggtcac cttcgacccg aagacgcgct cgacgagatt
     5341 gtgcggttct tcgaggcgcg ggtttccgcc ttgcgacgga gcggggtcgc tgccgaccgg
     5401 ctcatcctcg atccggggat gggatttttc ttgagccccg caccggaaac atcgctgcac
     5461 gtgctgtcga accttcaaaa gctgaagtcg gcgttggggc ttccgctatt ggtctcggtg
     5521 tcgcggaaat ccttcttggg cgccaccgtt ggccttcctg taaaggatct gggtccagcg
     5581 agccttgcgg cggaacttca cgcgatcggc aatggcgctg actacgtccg cacccacgcg
     5641 cctggagatc tgcgaagcgc aatcaccttc tcggaaaccc tcgcgaaatt tcgcagtcgc
     5701 gacgccagag accgagggtt agatcatgcc tagcattcac cttccggccg cccgctaaat
     5761 atctcctttt gggttgttaa taaaacatcc aataagttga ctgtgcgtga aaaagaaagt
     5821 tttgtgtgat ggcgttgaag atcgcaccgt taagctctta tgtgggatgg tgcagagctc
     5881 gacgactacc gataaaacgc aaccgccgca aacagacaag aaaaagcccc aactgataac
     5941 agttggggct tcagtattgt gattggtgga gcaatagcac cctgaaccca aaaccttctc
     6001 gctcaaccgg tagtggctga taacaactcg tgagggctat tgcgggttaa gcatttagcg
     6061 atgtctaggg ccagactgga cgtctgaacg caagccgctg atactgtaca taaccacagt
     6121 atcagcggag gatacccatg tcgctggcaa ggaacgccac ggcgagtcaa tcgcccactc
     6181 aaacaaacgg ttacgaacgc caccaacccg accagacgct gctctaccag ctggttgagc
     6241 agcactaccc agccttcaaa gcctcactcg aagcccaagg tcaacacctg cctcgctaca
     6301 tccaacaaga attcaacgac ctcctccaat gtggccgtct ggagtatggt ttcatgcggg
     6361 ttcgctgcga ggattgtcat cacgagcgtc tggtcgcctt cagctgtaaa cgacgcggct
     6421 tttgccctag ctgcggtgcc cgccggatgg ccgagagtgc ggcgctgctg atagacgaag
     6481 tcttccccaa ggagcccatt cgccagtggg tgctcagctt tcctttccag ctacgctttt
     6541 tgctggctcg ccatccccag ctgatgggcc aggtcttgag tatcgtctat cgtacactct
     6601 caactcatct gatcaaaaaa gccggttaca ccaaagcctc tgcacaaact ggctcagtga
     6661 ctcttatcca acgctttggc tccgcgctaa atctcaatgt ccactaccac atgctgtttc
     6721 tcgatggtgt ctatgccgaa gatgactatg gcaagcaacg cttccatcgt gtcaaggcac
     6781 ccacttacga tgagctgaat acgctcgctc acaccctcag ccatcgcatc gctcgctgca
     6841 tggaaaagcg tgggattttg gagcgtgatg ccgagaatac gtggttgaca ctggaagagg
     6901 gcgaagacga tacgctgact caattacatg gtgcttcggt tacgtatcgc attgccgtcg
     6961 gcccccagca agggcgcaaa gtcttcaccc tgcaaacctt gccagggcgt gaggataaag
     7021 ccgactcaag cagtcgagta gccaaccatg ctggtttctc gctacacgcc ggtgtgatgg
     7081 ccgaagcgca tcagcgggat aagcttgagc gcttgtgtcg ctacattagt cggccagcgg
     7141 tttcagaaaa acgtctggca ttaaccgcca atgggcaggt gcgttacgag ctcaaaactc
     7201 cgtaccgcaa tggcaccacc catgtgatct tcgagccgct ggacttcatc gccaaactcg
     7261 ctgcgttggt acctaagccg cgagtcaacc tcacacgctt ccacggcgtc tttgcaccga
     7321 acagcaaaca ccgagttcaa gtaacacccg ccaagcgggg caagaagccc gacaaatcgg
     7381 aaggtctcga tactaactgg cgtgacaaga gtcctgcaga gcgccaccgc gccatgacct
     7441 ggatgcaacg cctcaagcga gtcttcaata ttgatattga agtctgcgaa cactgcggcg
     7501 gtcacgtcaa agtgattgcc agcatcgaag atccgaaggt cattgagcag attctcaagc
     7561 atctgaaaca gaaaacagcc aaggcgaatg ccgccaagca gcgtgagctg ccaccagaac
     7621 gagcgccgcc actgactccc agcctgttcg atccatcaca gagtcgtctc tttgactgac
     7681 gaccccaaat ccaacactgc tcaacactgc caacttttaa acggggcggt ggggcagttt
     7741 gtatctctcg agctatcagg ctagagattt taccgccaaa tcgaacctta ttagagcggt
     7801 ttaggctgga ccggcagtta aaattggggc ttgagcggta aacgagtgag ggaatttcag
     7861 gtaagatact tcggatgagg agcaaaaagg tggtttatac ttcctatacc cccctccctg
     7921 attaaaggaa gccgtatgga tattattgat aaagtttttc agcaagagga tttctcacgc
     7981 caggatttga gtgacagccg ttttcgccgc tgccgctttt atcagtgtga cttcagccac
     8041 tgtcagctgc aggatgccag tttcgaggat tgcagtttca ttgaaagcgg cgccgttgaa
     8101 gggtgtcact tcagctatgc cgatctgcgc gatgccagtt tcaaggcctg ccgtctgtct
     8161 ttggccaact tcagcggtgc caactgcttt ggcatagagt tcagggagtg cgatctcaag
     8221 ggcgccaact tttcccgggc ccgcttctac aatcaagtca gccataagat gtacttctgc
     8281 tcggcttata tctcaggttg caacctggcc tataccaact tgagtggcca atgcctggaa
     8341 aaatgcgagc tgtttgaaaa caactggagc aatgccaatc tcagcggcgc ttccttgatg
     8401 ggctcagatc tcagccgcgg caccttctcc cgcgactgtt ggcaacaggt caatctgcgg
     8461 ggctgtgacc tgacctttgc cgatctggat gggctcgacc ccagacgggt caacctcgaa
     8521 ggagtcaaga tctgtgcctg gcaacaggag caactgctgg aacccttggg agtaatagtg
     8581 ctgccggatt agctcgaatg caaacacaag aggcaaatat cacttgagtt ccgcgctaca
     8641 acaaggcaga ttctgctata gcagagtatt accgccggaa tgccgtttct ctgctgccgc
     8701 agccgacgtt ataccttccc ttcaaaggag gcattatgaa gactcaactg ccctaaagaa
     8761 aaacttacag gtggattatg gtcagacgtt atctccccct taacccgctg cgcgcctttg
     8821 aggccgccgc ccgtcatctc agttttaccc gcgcggcgat tgagctgaat gtcacccatg
     8881 ccgccgtcag ccagcaggtc agggcgctgg aagaacaact cggctgtgtg ctgtttaccc
     8941 gcgtctcgcg cgggctggtg ctgacccatg aaggtgaggg attactgccg gtgctcaatg
     9001 aggcgtttga ccggattgcg gatactctgg agtgtttttc tcacgggcag ttccgtgagc
     9061 gggtgaaagt cggtgcggtg ggaacatttg ccgcaggctg gctgctgccg cgtctggccg
     9121 gattctatga cagccatccg catattgatc tgcatatctc cacccataac aatcatgtgg
     9181 acccggcggc ggaagggcat gattatacga tccgtttcgg taacggcgcg tggcatgagt
     9241 cagatgcgga actgattttc agtgcaccac acgctccgct gtgctcaccg gccattgcag
     9301 aacagttaca gcagccggat gatgttcacc gctttaccct gctgcgctca ttccgccggg
     9361 atgaatggag ccgctggctg gattgtgcgg gcggcacacc gccttccccg tcacagccgg
     9421 taatggtgtt cgatacctca ctggccatgg ccgaggcggc acaactgggt gccggggtag
     9481 cgatcgcacc ggtatgtatg ttcagccgcc tgttacagtc aggcgcactg gtacagccgt
     9541 ttgccgcaga aatcaccctc ggcggctact ggctgacgcg gttacagtcc cgtacggaaa
     9601 ccccggccat gcagcaattc gcccgctggc tgctgaatac ggcggcggcg taaaactcac
     9661 tcaccttcca ggctttttac ccgcaaatca tcaccttcac tgatgcgcag ccgttcactg
     9721 ccgcagtgcg gacagcatcc ggcgtgctgc atgatttcgg cctcacggct gcaatcccag
     9781 caccatgcct gtgccgggat aacatcaata tgcagtgtgc agccctgcgc cacggtatca
     9841 cggcaggcga tatcaaaaca gaaatgcagt gcactctcct caacatccgc cagtgcgccg
     9901 acttccagcc agacatccgt tacccgcgca atgccgtgct gttcagcctg ttcgcggata
     9961 atgtccgccg caccgagggc tttactaagc ttgccccttc cgccgttgtc ataatcggtt
    10021 atggcatcgc attttatttt ctttctctgg ttctgaaatc catccctgtc ggtgttgctt
    10081 atgcagtctg gtcgggactc ggcgtcgtca taattacagc cattgcctgg ttgcttcatg
    10141 ggcaaaagct tgatgcgtgg ggctttgtag gtatggggct cataattgct gcctttttgc
    10201 tcgcccgatc cccatcgtgg aagtcgctgc ggaggccgac gccatggtga cggtgttcgg
    10261 cattctgaat ctcaccgagg actccttctt cgatgagagc cggcggctag accccgccgg
    10321 cgctgtcacc gcggcgatcg aaatgctgcg agtcggatca gacgtcgtgg atgtcggacc
    10381 ggccgccagc catccggacg cgaggcctgt atcgccggcc gatgagatca gacgtattgc
    10441 gccgctctta gacgccctgt ccgatcagat gcaccgtgtt tcaatcgaca gcttccaacc
    10501 ggaaacccag cgctatgcgc tcaagcgcgg cgtgggctac ctgaacgata tccaaggatt
    10561 tcctgaccct gcgctctatc ccgatattgc tgaggcggac tgcaggctgg tggttatgca
    10621 ctcagcgcag cgggatggca tcgccacccg caccggtcac cttcgacccg aagacgcgct
    10681 cgacgagatt gtgcggttct tcgaggcgcg ggtttccgcc ttgcgacgga gcggggtcgc
    10741 tgccgaccgg ctcatcctcg atccggggat gggatttttc ttgagccccg caccggaaac
    10801 atcgctgcac gtgctgtcga accttcaaaa gctgaagtcg gcgttggggc ttccgctatt
    10861 ggtctcggtg tcgcggaaat ccttcttggg cgccaccgtt ggccttcctg taaaggatct
    10921 gggtccagcg agccttgcgg cggaacttca cgcgatcggc aatggcgctg actacgtccg
    10981 cacccacgcg cctggagatc tgcgaagcgc aatcaccttc tcggaaaccc tcgcgaaatt
    11041 tcgcagtcgc gacgccagag accgagggtt agatcatgcc tagcattcac cttccggccg
    11101 cccgctaaat atctcctttt gggttgcgcg aaggtgggcg cagacatgct gggctcgtca
    11161 ggatcaaact gcactatgag gcggcggttc ataccgcgcc aggggagcga atggacagcg
    11221 aggagcctcc gaacgttcgg gtcgcctgct cgggtgatat cgacgaggtt gtgcggctga
    11281 tgcacgacgc tgcggcgtgg atgtccgcca agggaacgcc cgcctgggac gtcgcgcgga
    11341 tcgaccggac attcgcggag accttcgtcc tgagatccga gctcctagtc gcgagttgca
    11401 gcgacggcat cgtcggctgt tgcaccttgt cggccgagga tcccgagttc tggcccgacg
    11461 ccctcaaggg ggaggccgca tatctgcaca agctcgcggt gcgacggaca catgcgggcc
    11521 ggggtgtcag ctccgcgctg atcgaggctt gccgccatgc cgcgcgaacg caggggtgcg
    11581 ccaagctgcg gctcgactgc cacccgaacc tgcgtggcct atacgagcgg ctcggattca
    11641 cccacgtcga cactttcaat cccggctggg atccaacctt catcgcagaa cgcctagaac
    11701 tcgaaatcta acgtccgttc gggcatcgag gtccatgtcg gggtgggacg ggcccgtggc
    11761 ttcaagatca cttgcagtcc gaccgcgatg tcttggttgc gcgagaggtt gtcgatatcc
    11821 tccacttcca tcatcaaccc tggataatgc cgccgccgtc atcgccgccg acgcccgtgc
    11881 cgggcttttc gggcctgtca ggcttgctcg gccttcagcc tgcctgggcg agatctccgg
    11941 cggacggatt aacggcggag cttcgccgcc tttcgtgcgt gtgaaggccg aagatagttc
    12001 tctcaaaaac atccgtttat gagagatacc aaatgtcatt ttcagaagac gactgcacca
    12061 gttgattggg cgtaatggct gttgtgcagc cagctcctga cagttcaata tcagaagtga
    12121 tctgcaccaa tctcgactat gctcaatact cgtgtgggct ctgttgcaaa aatcgtgaag
    12181 cttgagcatg cttggcggag attggacgga cggaacgatg acggatttca agtggcgcca
    12241 tttccagggt gatgtgatcc tgtgggcggt gcgctggtat tgtcgctatc cgatcagcta
    12301 tcgcgacctt gaggaaatgc tggcggaacg cggcatttcg gtcgaccata cgacgatcta
    12361 tcgctgggtc cagtgctacg ccccggagat ggagaagcgg ctgcgctggt tctggcggcg
    12421 tggctttgat ccgagctggc gcctggatga aacctacgtc aaggtgcggg gcaagtggac
    12481 ctacctgtac cgggcagtcg acaagcgggg cgacacgatc gatttctacc tgtcgccgac
    12541 ccgcagcgcc aaggcagcga agcggttcct gggcaaggcc ctgcgaggcc tgaagcactg
    12601 ggaaaagcct gccacgctca ataccgacaa agcgccgagc tatggtgcag cgatcaccga
    12661 attgaagcgc gaaggaaagc tggaccggga gacggcccac cggcaggtga agtatctcaa
    12721 taacgtgatc gaggccgatc acggaaagct caagatactg atcaagccgg tgcgcggttt
    12781 caaatcgatc cccacggcct atgccacgat caagggattc gaagtcatgc gagccctgcg
    12841 caaaggacag gctcgcccct ggtgcctgca gcccggcatc aggggcgagg tgcgccttgt
    12901 ggagagagct tttggcattg ggccctcggc gctgacggag gccatgggca tgctcaacca
    12961 ccatttcgca gcagccgcct gatcggcgca gagcgacagc ctacctctga ctgccgccaa
    13021 tctttgcaac agagcctccg tcgccatgct cacctcgctt tggtgcacac gagtattgag
    13081 catagtcgag attggtgcag atcacttctg atattgaact gtcaggagct ggctgcacaa
    13141 cagccattac gcccaatcaa ctggtgcagt cgtcttctga aaatgaca
//
