LOCUS       In363                  15892 bp    DNA     linear       22-OCT-2020
DEFINITION  Salmonella enterica subsp. enterica.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica
  ORGANISM  Salmonella enterica subsp. enterica
            Unclassified.
REFERENCE   1  (bases 1 to 15892)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of In363
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15892)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15892
                     /organism="Salmonella enterica subsp. enterica"
                     /mol_type="genomic DNA"
     mobile_element  1..15892
                     /gene="In363"
                     /product="Concise class 1 integron: In363"
     repeat_region   1..25
                     /gene="IRi_In363"
                     /product="Inverted repeat at the integrase end of In363"
     CDS             complement(203..1216)
                     /gene="intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="Integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     regulatory      1101..1129
                     /gene="PcWTGN-10"
                     /product="Promoter PcWTGN-10"
     regulatory      1101..1106
                     /gene="-35 region_PcWTGN-10"
                     /product="-35 region of PcWTGN-10"
     regulatory      1121..1122
                     /gene="extended_-10"
                     /product="Extended -10 region"
     regulatory      1124..1129
                     /gene="-10 region_PcWTGN-10"
                     /product="-10 region of PcWTGN-10"
     misc_recomb     1297..1359
                     /gene="attI1"
                     /product="attI1 site"
     CDS             1373..1846
                     /gene="dfrA1"
                     /codon_start=1
                     /transl_table=11
                     /product="Trimethoprim-insensitive dihydrofolate
                     reductase"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     misc_recomb     1841..1929
                     /gene="attC_dfrA1"
                     /product="attC site for dfrA1"
     CDS             1940..2419
                     /gene="gcuC"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MKIVHYEANAPWIGRMKCPNPKCGKETPAWQSSGMSDSCPHFFC
                     DTCSNVIHREQDHALLYENEINQELLDRIAATLPDCPCGGRFVPGANPKCPSCKTEYV
                     HQWDAVKRLNVPFMPILDGSCLIRDRLYSYEVCIGSKPKYWWRLFTNALTSLGKGRS"
     misc_recomb     2388..2441
                     /gene="attC_gcuC"
                     /product="attC site for gcuC"
     CDS             2550..2897
                     /gene="qacED1"
                     /codon_start=1
                     /transl_table=11
                     /product="Quarternary ammonium coumpounds resistance
                     protein"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     misc_feature    2891..3419
                     /gene="truncated sul1"
                     /product="Truncated sulfonamide resistance protein"
     misc_feature    3420..3854
                     /product="Hypothetical protein"
     CDS             4079..5293
                     /gene="cmlA9"
                     /codon_start=1
                     /transl_table=11
                     /product="Chloramphenicol efflux protein CmlA9"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGVL
                     NTTPSIIQLTLSLYMVMLGVGQVIFGPLSDRVGRRPILLVGATAFVAASLGAACSSTA
                     LAFVAFRLVQAVGASAMLVATFATVRDVYANRPEGAVIYGLFSSMLAFVPALGPIAGA
                     LIGEFWGWQAIFITLAALASLALLNASFRWHETRPLDQARTQRSVLPIFASPAFWVYT
                     VGFSAGMGTFFVFFSTAPRVLIGQAGYSEIGFSLAFATVALVMVTTTRFAKSFVAKWG
                     IAGCVARGMALLVSGAILLGIGQLFGSPSFFSFILPMWVVAVGIVFTVSVTANGALAQ
                     FDDIAGSAVAFYFCIQSLIVSIVGTLAVTLLNGDTAWPVICYATAMAVLVSLGLALLR
                     SRDAATEKSPVV"
     CDS             complement(5500..6126)
                     /gene="tetR(G)"
                     /codon_start=1
                     /transl_table=11
                     /product="Tetracycline resistance regulator protein, class
                     G"
                     /translation="MTKLDKGTVIAAALELLNEVGMDSLTTRKLAERLKVQQPALYWH
                     FQNKRALLDALAEAMLAERHTRSLPEENEDWRVFLKENALSFRTALLSYRDGARIHAG
                     TRPTEPNFGTAETQIRFLCAEGFCPKRAVWALRAVSHYVVGSVLEQQASDADERVPDR
                     PDVSEQAPSSFLHDLFHELETDGMDAAFNFGLDSLIAGFERLRSSTTD"
     CDS             6278..7405
                     /gene="tetA(G)"
                     /codon_start=1
                     /transl_table=11
                     /product="Tetracycline resistance protein, class G"
                     /translation="MGLGLIMPVLPTLLRELVPAEQVAGHYGALLSLYALMQVVFAPM
                     LGQLSDSYGRRPVLLASLAGAAVDYTIMASAPVLWVLYIGRLVSGVTGATGAVAASTI
                     ADSTGEGSRARWFGYMGACYGAGMIAGPALGGMLGGISAHAPFIAAALLNGFAFLLAC
                     IFLKETHHSHGGTGKPVRIKPFVLLRLDDALRGLGALFAVFFIIQLIGQVPAALWVIY
                     GEDRFQWNTATVGLSLAAFGATHAIFQAFVTGPLSSRLGERRTLLFGMAADATGFVLL
                     AFATQGWMVFPILLLLAAGGVGMPALQAMLSNNVSSNKQGALQGTLTSLTNLSSIAGP
                     LGFTALYSATAGAWNGWVWIVGAILYLICLPILRRPFATSL"
     CDS             7426..8217
                     /gene="lysR"
                     /codon_start=1
                     /transl_table=11
                     /product="Putative LysR-type transcriptional regulator"
                     /translation="MRRLRRNDGLNLLSNHLLSGRVPMMTLVHTLAVAEYLNFRHAAN
                     ALGVAQSSVSARVKALEEDLGILLFERHARGVRLTEAGRHFVERIAVGIDQLDHAVKT
                     AGMAAAGESGRLRIGIHALIPHSFLAKLIGQYRKDYPDVEVEIAEGPAREAVVQLRAG
                     RLDVAFVAGTPQPPDCHSRRTWTEPLLAVLPERHPLAKRSAVTWPDLAGETFLAYRKF
                     KRPFRAVCWVGGGRASPVLGTDRTSVSGISASRGHAAALSAAMLR"
     mobile_element  8065..9793
                     /gene="ISCR3"
                     /product="Insertion sequence: ISCR3"
     repeat_region   complement(8065..8091)
                     /gene="oriIS"
                     /product="ISCR3 oriIS"
     CDS             complement(8309..9793)
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCR3 transposase"
                     /translation="MPSAHTGSRYARHAPERTLLYALVEAHYPDFIARIEAEGRSLPG
                     YVREAFDAYLRCGVLEHGFLRVVCEHCRAERLVAFSCKKRGFCPSCGARRMAESARHL
                     VEEVFGPRPVRQWVLSFPYPLRFLFASKPEAIGPVLGIVQRVIAGWLADQAGIDRASA
                     QCGAVTLIQRFGSALNLNIHFHMLWLDGVYVEASELPRRELRLHRARAPTTAQLTQLA
                     ATIAHRVCRHLTRKGWLEGEGESAFLADSAAGDDSMDGLRMSSITYRIATGRDAGCKV
                     VTLQTLPGDAGSLEGEAGKVGGFSLHAGVAAEAHESHKLEKLCRYITRPAISEKRLSI
                     ALQGRVRYQLKTPWRNGTTHVEWDPVDFIAKLAALVPPPRAHLTRFHGVFAPNANLRA
                     QLTPSGRGKRPAGDAAPVDVSAHDAPRSPEEKRRAMSWAQRLKRVFSIDVTACVHCGG
                     TVRIVASIEEPTAIRAILAHFEKHGAREEAHYRPAARAPPVQAA"
     CDS             complement(10068..10721)
                     /gene="truncated intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="GroEL/integrase fusion protein"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWQG
                     INEDQNLGIAITRRALEAPLRAIVANAGEEPSVIVANVKAGEGSYGYNAATGEFGDMI
                     AMGILDPTKVTRSALQHAASVAGLAITTEVVVAEVPKKEEPAMPGAGGMGGMGGMDF"
     regulatory      10606..10634
                     /gene="PcWTGN-10"
                     /product="Promoter PcWTGN-10"
     regulatory      10606..10611
                     /gene="-35 region_PcWTGN-10"
                     /product="-35 region of PcWTGN-10"
     regulatory      10626..10627
                     /gene="extended_-10"
                     /product="Extended -10 region"
     regulatory      10629..10634
                     /gene="-10 region_PcWTGN-10"
                     /product="-10 region of PcWTGN-10"
     misc_recomb     10802..10838
                     /gene="truncated attI1"
                     /product="Truncated attI1 site"
     CDS             10823..11674
                     /gene="sul3"
                     /codon_start=1
                     /transl_table=11
                     /product="Sulfonamide resistance protein"
                     /translation="MLRSRVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSD
                     VVDVGPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVG
                     YLNDIQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEAR
                     VSALRRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSF
                     LGATVGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDAR
                     DRGLDHA"
     CDS             11802..12302
                     /gene="orf5"
                     /codon_start=1
                     /transl_table=11
                     /product="Putative acetyltransferase"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     CDS             12326..12613
                     /gene="orf6"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MSGWDGPVASRSLAVRPRCLGCARGCRYPPLPSSTLDNAAAVIA
                     ADARAGLFGPVRLARPSACLGEISGGRINGGASPPFVRVKAEDSSLKNIRL"
     repeat_region   complement(12625..12649)
                     /gene="IRt_In0"
                     /product="Inverted repeat at the tni end of In0"
     mobile_element  12748..13627
                     /gene="IS6100"
                     /product="Insertion sequence: IS6100"
     repeat_region   12748..12761
                     /gene="IRL_IS6100"
                     /product="IS6100 inverted repeat left"
     CDS             12779..13573
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6100 transposase"
                     /translation="MLGGDWTDGTMTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEM
                     LAERGISVDHTTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYR
                     AVDKRGDTIDFYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELK
                     REGKLDRETAHRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALR
                     KGQARPWCLQPGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     repeat_region   13614..13627
                     /gene="IRR_IS6100"
                     /product="IS6100 inverted repeat right"
     repeat_region   13755..13779
                     /gene="IRt_In0"
                     /product="Inverted repeat at the tni end of In0"
     misc_feature    complement(13780..14064)
                     /gene="truncated tniB"
                     /product="Truncated ATP-binding protein TniB (pseudogene)"
     CDS             complement(14067..15749)
                     /gene="tniA"
                     /codon_start=1
                     /transl_table=11
                     /product="DD(35)E transposase TniA"
                     /translation="MASDTSLIAEQGVATLPDAAWAQARQRAEIIGPLAALDVVGHEA
                     ADAAAHALGLSRRQVYVLIRRARQGAGLVTDLARSRSGGGKGKGRLPESVERIIRELL
                     QKRFLTKQKRSLAAFHREVAQACKAQKLRAPARNTVALRIAGLDPLKATRRREGQDAS
                     RSLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDERDRQPIGRPYLTIAIDVFTRCVLG
                     MVVTLEAPSSVSVGLCLVHVACDKRPWLEGLNIEMEWPMSGKPRLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAIARTGVPTVITRTTAFLV
                     DFLPIIRRTLTRTGFVIDHIHYYADALKPWIARRDRLPAFLIRRDPRDISRIWVLEPE
                     GQHYLEIPYRTLSHPAVTLWEQRQALAKLRQQGREQVDESALFRMIGQMREIVSTAQK
                     ATRKARRDADRRQHLKATAVLFKTTPPPDADMADPQADNQPPAKPFDQIEEW"
     repeat_region   15868..15892
                     /gene="IRt_In363"
                     /product="Inverted repeat at the tni end of In363"
BASE COUNT     2991 a   4705 c   4852 g   3344 t
ORIGIN
        1 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
       61 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
      121 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
      181 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
      241 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
      301 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
      361 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
      421 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
      481 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
      541 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
      601 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
      661 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
      721 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
      781 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
      841 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
      901 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
      961 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     1021 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     1081 ggatgaaggc acgaacccag tggacataag cctgttcggt tggtaagctg taatgcaagt
     1141 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1201 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat
     1261 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1321 ttacgcagca gggcagtcgc cctaaaacaa agttaacctc tgaggaagaa ttgtgaaact
     1381 atcactaatg gtagctatat cgaagaatgg agttatcggg aatggccctg atattccatg
     1441 gagtgccaaa ggtgaacagc tcctgtttaa agctattacc tataaccaat ggctgttggt
     1501 tggacgcaag acttttgaat caatgggagc attacccaac cgaaagtatg cggtcgtaac
     1561 acgttcaagt tttacatctg acaatgagaa cgtattgatc tttccatcaa ttaaagatgc
     1621 tttaaccaac ctaaagaaaa taacggatca tgtcattgtt tcaggtggtg gggagatata
     1681 caaaagcctg atcgatcaag tagatacact acatatatct acaatagaca tcgagccgga
     1741 aggtgatgtt tactttcctg aaatccccag caattttagg ccagttttta cccaagactt
     1801 cgcctctaac ataaattata gttaccaaat ctggcaaaag ggttaacaag tggcagcaac
     1861 ggattcgcaa acctgtcacg ccttttgtac caaaagccgc gccaggtttg cgatccgctg
     1921 tgccaggcgt taaggctaca tgaaaatcgt acattacgaa gcgaatgcac catggatagg
     1981 aagaatgaaa tgcccaaacc caaagtgtgg gaaggaaact cctgcctggc aatcgagcgg
     2041 catgagcgac agttgcccgc attttttctg tgatacttgc tcgaatgtaa tccatagaga
     2101 gcaggaccat gcattactgt atgaaaatga aatcaatcaa gagctcttgg atcgaatagc
     2161 agcaactctt ccagattgcc cttgcggggg taggtttgtt cctggtgcaa acccaaagtg
     2221 tccgagttgc aagaccgagt acgtgcacca atgggatgca gtgaaaaggt tgaatgtacc
     2281 ttttatgcca atcttggatg gttcctgctt gattcgagat aggctgtatt cgtatgaagt
     2341 atgcattggt tctaaaccaa aatactggtg gcgtttgttc acaaatgcct taacaagttt
     2401 aggcaaggga cgctcctgac gtcgcgcccc tgctaaaagc gttagatgca ctaagcacat
     2461 aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa
     2521 taagccctac acaaattggg agatatatca tgaaaggctg gctttttctt gttatcgcaa
     2581 tagttggcga agtaatcgca acatccgcat taaaatctag cgagggcttt actaagcttg
     2641 ccccttccgc cgttgtcata atcggttatg gcatcgcatt ttattttctt tctctggttc
     2701 tgaaatccat ccctgtcggt gttgcttatg cagtctggtc gggactcggc gtcgtcataa
     2761 ttacagccat tgcctggttg cttcatgggc aaaagcttga tgcgtggggc tttgtaggta
     2821 tggggctcat aattgctgcc tttttgctcg cccgatcccc atcgtggaag tcgctgcgga
     2881 ggccgacgcc atggtgacgg tgttcggcat tctgaatctc accgaggact ccttcttcga
     2941 tgagagccgg cggctagacc ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt
     3001 cggatcagac gtcgtggatg tcggaccggc cgccagccat ccggacgcga ggcctgtatc
     3061 gccggccgat gagatcagac gtattgcgcc gctcttagac gccctgtccg atcagatgca
     3121 ccgtgtttca atcgacagct tccaaccgga aacccagcgc tatgcgctca agcgcggcgt
     3181 gggctacctg aacgatatcc aaggatttcc tgaccctgcg ctctatcccg atattgctga
     3241 ggcggactgc aggctggtgg ttatgcactc agcgcagcgg gatggcatcg ccacccgcac
     3301 cggtcacctt cgacccgaag acgcgctcga cgagattgtg cggttcttcg aggcgcgggt
     3361 ttccgccttg cgacggagcg gggtcgctgc cgaccggctc atcctcgatc cggggatgga
     3421 ccggcgattg gtccatggcg aaacggccga ccttgcgccg accggcttcg ggcaacaggt
     3481 ccgcgaagcc atggaccagc gccgcgagca tcatatcgaa cagcgcgacg ccacgcgcaa
     3541 cagggacggc cgaatcttct accggcgcaa tcttctcgcc accctgcgcg agcgggaagt
     3601 tgcgcgcgcc ggtgcggaga tggccgaggg caaggcgctg ccgttccgcg ccgccaagga
     3661 tggtgagagt gtcagcggca agttcaccgg gactgtccag ctaacgagcg gcaagttcgc
     3721 catcgtggaa aagagccacg agttcaccct tgtcccgtgg cggccgatca tcgaccgcca
     3781 gctcggccgc gaggtcgcgg gtatcatgca gggcggttcg gtgtcgtggc agttagggcg
     3841 gcagcggggg ttggggctat aggagccaac gataccgcat tgcaacgcaa caactaattc
     3901 gataagatct gctattcaat ttcgtaatcg tggagccatc agcatgggaa attccaagtc
     3961 agcagacaag taagccgcaa catcagaatt gttgttgcgg cgctctgtaa gaccaatccc
     4021 atctgattgc tgacgagcag acgctgcccg gtatccttaa tcgagaggtt gattcgtcat
     4081 gaccaccaca cgccccgcgt gggcctatac gctgccggca gccttgctgc ttatggctcc
     4141 cttcgacatc ctcgcctcgc tggcgatgga tatttatctt ccagtcgttc cggcgatgcc
     4201 gggcgtcctg aacacgactc catccataat ccaactcacg ttgagcctct acatggtgat
     4261 gctcggtgtg ggccaagtga tctttgggcc actctccgat cgcgtcgggc gacggccgat
     4321 cctgcttgta ggcgcaacgg ctttcgttgc tgcgtctctg ggagcggctt gttcttcaac
     4381 tgcattagcc tttgttgcgt ttcgtctggt tcaggctgtt ggagcatcgg ccatgctggt
     4441 ggccaccttc gcgaccgtgc gcgacgtata tgccaatcgt cccgaaggtg ccgtcatcta
     4501 cggccttttc agttcgatgc tggcgttcgt gcctgcgctc ggccctatag ccggtgcgct
     4561 gatcggcgag ttttggggat ggcaggcgat cttcatcaca ctggctgcac tggcttcgct
     4621 cgcactctta aacgccagtt tcaggtggca tgaaacccga ccgttggatc aggccagaac
     4681 gcaacgatct gttttgccga tcttcgcgag tccggccttt tgggtttaca cggtcggatt
     4741 tagtgccggc atgggcacat tcttcgtttt cttctcgaca gccccccgtg ttctcatagg
     4801 ccaagccggc tattccgaga tcggatttag cttggccttc gcgactgtcg cgctggtcat
     4861 ggtcacgaca acccgcttcg caaagtcctt cgttgccaaa tggggtatcg cgggatgcgt
     4921 agcgcgcggg atggcgttgc tcgtttccgg cgcgatcctg ttggggatcg gccaactttt
     4981 cggatcgccg tcatttttca gcttcatcct gccgatgtgg gttgtcgcgg tcggcattgt
     5041 cttcacggtg tccgttaccg ccaacggcgc acttgcgcag ttcgacgaca tcgctggatc
     5101 agcggttgcg ttctacttct gcatccaaag cctgatagtc agtatcgtcg ggacattggc
     5161 ggtgacgctg ttaaacggcg atacagcgtg gcccgtgatt tgttacgcca cggcaatggc
     5221 agtgctggtg tcgttggggc tggcgctcct tcgatcccgt gatgctgcca ccgagaagtc
     5281 gccagtcgtc tagccgacga ctggaagcaa gcccgctccg atgcggcgca ataatcttcg
     5341 aaacctcgtg aatggcggta tcctgtctgg caagataccg ctcatttccc ttgtcccgtg
     5401 gcggccggtc atcgaccgcc agctcggccg tgaggtcatg ggcatcgtgc aaagcggatc
     5461 ggtgtcgtgg cagttggggc ggcaaagggg cataagcctc taatctgttg tagatgaacg
     5521 cagccgctcg aaaccagcga tgaggctgtc gagtccgaag ttgaacgcag catccatgcc
     5581 gtctgtttcc aactcgtgaa acagatcgtg caggaaggac gacggtgctt gctcggacac
     5641 atctggcctg tccggaactc tctcatcggc atcagatgcc tgctgctcga gaacggaacc
     5701 gaccacatag tgactgaccg cccggagcgc ccaaacggcg cgcttcggac aaaagccctc
     5761 cgcgcagaga aagcgtattt gcgtctcggc ggtgccaaaa ttcggttctg tcggtcgagt
     5821 gccggcatgg atacgcgcgc cgtcccgata agagagcaac gccgttctga agctcagggc
     5881 attctctttc aggaacaccc gccagtcctc attctcttcg ggtagcgagc gggtatggcg
     5941 ttccgccagc atcgcctcgg cgagcgcatc aagcagcgct cgcttgttct ggaaatgcca
     6001 gtaaagcgca ggctgctgaa ccttgaggcg ttcagcgagc ttccgcgtcg tcaggctgtc
     6061 catgccaacc tcgttcaaca gctctagcgc cgccgcgatc acggtgccct tgtccagttt
     6121 ggtcattcac gttccttcgc cagtgcttga caatttatca ccgataagtt atatgtccat
     6181 ctccttatcg ttgataaagt cgctccattg agcggcgctg gagtttcagg tgcgcagctc
     6241 tgccatcatt gccctgctga tcgtgggtct tgacgccatg ggtctcggcc tcatcatgcc
     6301 cgtccttccg acgcttctgc gtgagcttgt gccagcagag caggtcgctg gacactatgg
     6361 tgccttgctg tcgctctatg cattgatgca ggtcgtcttc gcgcccatgc ttggacagct
     6421 ttcggattct tacggtcggc gtccggtact tctggcttct cttgcaggag ccgcagtcga
     6481 ttacacgatt atggcatcag cgccggtctt atgggtgctc tatatcggcc gactcgtgtc
     6541 cggcgtcacg ggcgcaaccg gagctgtagc agcctcaacc attgccgatt cgacggggga
     6601 aggttctcgc gcacgctggt tcggctacat gggggcctgt tatggggcgg gcatgattgc
     6661 cgggccagca cttggtggca tgctcggtgg tatctctgct catgccccgt ttatcgccgc
     6721 cgcccttctc aacgggttcg cgttcctgct tgcctgcatt ttcctcaagg agactcatca
     6781 cagccatggc gggaccggaa agccggttcg catcaaacca ttcgttctgt tacggctgga
     6841 tgatgcattg cgcgggctag gtgcgctttt cgcagttttc ttcattattc aactgatcgg
     6901 ccaagtgcct gcagccctat gggtcatata tggcgaggac cgttttcagt ggaacaccgc
     6961 gaccgttggt ttgtcgctcg cggcgtttgg ggcaacacat gcgatcttcc aagcgtttgt
     7021 taccggcccg ctttcaagcc ggcttggaga gcggcgcacg ctgctgtttg gcatggctgc
     7081 ggatgcgact ggcttcgttc ttctggcttt tgccacgcag ggatggatgg tgttcccgat
     7141 tctgttgctg cttgccgccg ggggtgttgg catgccggcc ttgcaggcaa tgctctcaaa
     7201 caatgtcagc agtaacaagc aaggggcttt gcaaggaacg ctaacgagcc tcaccaatct
     7261 aagctctatc gcaggaccgc ttggcttcac agcactctat tctgccaccg ccggggcatg
     7321 gaacggttgg gtttggattg tcggcgcgat cctctattta atatgtctgc caatactacg
     7381 cagaccattc gcaacttcat tgtgatttag tcatggcgat ttggcatgcg tagacttagg
     7441 agaaatgacg gattaaatct gttgagcaat catctccttt cggggcgagt gccaatgatg
     7501 accttagttc acactctcgc tgtcgccgaa tatctcaact tccgtcacgc cgccaacgcg
     7561 ctcggcgttg cacagtccag cgtcagcgcc cgcgtgaagg cactggaaga agacctcggc
     7621 atcctcttgt tcgagcgtca tgcgcgcggc gttcggctga ccgaggccgg acgccatttc
     7681 gtcgagcgga tagccgtagg tattgaccaa ctcgaccatg cggtgaaaac cgccggcatg
     7741 gcggcagccg gagaaagcgg ccggcttcgt atcggtatcc atgccctgat tccgcatagc
     7801 ttcctcgcaa agctgatcgg ccaataccgc aaggattacc ccgatgttga agtcgagatc
     7861 gccgaaggcc cggcccgtga agcggtggtg cagcttcgcg ccggcaggtt ggacgtggcg
     7921 ttcgtcgcgg gcacgcccca accacccgac tgccattccc gtcgcacatg gaccgaaccg
     7981 ctcttggcgg tgctaccgga acggcatccg ctcgccaagc ggtcagccgt cacatggccc
     8041 gatttggcag gcgagacgtt ccttgcgtat aggaagttca aacgcccttt tcgggcagtc
     8101 tgctgggtag gcggcggtcg cgcaagcccc gttttgggca cggatcggac gtctgtgagt
     8161 gggatttcgg catcgcgggg ccacgcagcg gcgttgtcag cagccatgct tcgctgattc
     8221 ccgacagcgg gccgagcgcc gccctgcgga tcgtggccgc atcggctcgg attccggttt
     8281 cgccgtcggc tgtgcagccg gcagatcgtc acgcggcttg cactggcggc gcgcgcgctg
     8341 cgggcctgta gtgcgcttct tcccgcgcgc cgtgcttctc gaagtgggcg aggatggcgc
     8401 ggatggcggt gggttcctcg atgctggcga cgatccgcac ggtgccaccg cagtggacgc
     8461 aggcggtgac gtcgatggaa aagacccgct tgagccgttg cgcccagctc atcgcacggc
     8521 gcttctcctc ggggctgcgc ggcgcgtcgt gggcgctgac gtccactggc gccgcatcgc
     8581 ccgcaggccg cttgccgcgc cccgagggcg tcagctgcgc acgcaggttt gcattcgggg
     8641 cgaatacgcc gtggaagcgg gtgagatgcg cgcgaggtgg cgggaccagc gccgccagct
     8701 tggcgatgaa atccaccgga tcccattcca catgcgtggt gccattgcgc cacggggtct
     8761 tgagctggta acgcaccctg ccctggagcg ctatcgacag ccgcttctcg ctgatcgccg
     8821 ggcgcgtgat gtagcggcac agcttttcca gcttgtggct ttcgtgtgct tcggccgcca
     8881 cgccggcatg cagtgagaag ccgccgacct tgccggcttc gccctccagc gaaccggcgt
     8941 caccgggcag cgtttgcagc gtgacgacct tgcagccagc gtcgcggccg gtggcgatgc
     9001 ggtaggtgat cgaactcatc cgcagcccat ccatgctgtc gtcgcctgca gcgctgtctg
     9061 ccaggaaggc cgattcgccc tccccttcga gccagccttt gcgcgtcagg tgccgacaca
     9121 cccggtgcgc gatggtagct gccagctggg tcaactgcgc ggtggtgggc gcacgggcgc
     9181 ggtgcaggcg cagttcgcgc cgcggcagct cggaggcttc cacgtacacg ccgtcgagcc
     9241 acagcatgtg gaagtggatg ttcaggttca gcgcgctgcc gaaacgctgg atcagcgtca
     9301 ccgcgccgca ctgggcgctg gcgcggtcga tgccggcttg atcggccaac cagccggcga
     9361 tcacgcgctg cacgatgccc agcaccgggc caatggcttc tggcttgctg gcgaacagga
     9421 aacgcaaggg gtacggaaag ctcagcaccc attgccgcac aggccgcggg ccgaacacct
     9481 cctcgaccag gtgccgcgca ctctcggcca tgcgtcgcgc gccgcaactc gggcagaacc
     9541 cgcgcttctt gcaggagaag gccaccagcc tctctgcacg gcagtgctcg cacaccaccc
     9601 gcaggaagcc gtgctcgagt acgccgcaac gcaggtaggc atcgaacgcc tcgcggacat
     9661 acccgggcag cgagcggccc tccgcttcga tccgtgcaat gaagtccggg tagtgcgcct
     9721 ctaccaacgc gtacagcagc gtgcgctcgg gcgcgtggcg cgcgtaccgc gaaccggtgt
     9781 gggcggacgg cagtggcgcg catcccgcgg cttgccgccg ggatgtggcg aggcgcggca
     9841 cgcagcgctc cggtgcgggg acggctgctc agtgttgcgc ctgtgttcgc acgttcgtat
     9901 cggtgcgttc tgatcttcgc gtcagacatt gccgcggcgc gggcacaaca aaaagcccgg
     9961 catcgctgcc gggctccggc cccgtccttg gggccttgat gtcgggtcgt tgccgggatc
    10021 ggaccgcgct ggcgcggtcc ggttccctga cgaccgggcc aaccggatca gaaatccatg
    10081 ccgcccatgc cgcccatacc gccagcaccc ggcatggccg gctcttcctt cttcggcact
    10141 tcggccacga ccacttcggt cgtgatcgca aggccggcga cggaagcggc gtgctgcagg
    10201 gccgagcggg tcaccttggt cgggtccagg atgcccatgg cgatcatgtc gccgaactcg
    10261 ccggtggcgg cgttgtagcc gtagctgcct tcgccggcct tgacgttggc cacgatcacg
    10321 ctcggttctt caccggcgtt ggccacgatg gcgcgcagcg gggcttccag cgcacggcgg
    10381 gtgatggcga tgcccaggtt ctggtcttcg ttgatgccct gccagggcag atccgtgcac
    10441 agcaccttgc cgtagaagaa cagcaaggcc gccaatgcct gacgatgcgt ggagaccgaa
    10501 accttgcgct cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt
    10561 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt
    10621 tcggttggta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
    10681 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
    10741 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
    10801 ttgatgttat ggagcagcaa cgatgttacg cagcagggtg acggtgttcg gcattctgaa
    10861 tctcaccgag gactccttct tcgatgagag ccggcggcta gaccccgccg gcgctgtcac
    10921 cgcggcgatc gaaatgctgc gagtcggatc agacgtcgtg gatgtcggac cggccgccag
    10981 ccatccggac gcgaggcctg tatcgccggc cgatgagatc agacgtattg cgccgctctt
    11041 agacgccctg tccgatcaga tgcaccgtgt ttcaatcgac agcttccaac cggaaaccca
    11101 gcgctatgcg ctcaagcgcg gcgtgggcta cctgaacgat atccaaggat ttcctgaccc
    11161 tgcgctctat cccgatattg ctgaggcgga ctgcaggctg gtggttatgc actcagcgca
    11221 gcgggatggc atcgccaccc gcaccggtca ccttcgaccc gaagacgcgc tcgacgagat
    11281 tgtgcggttc ttcgaggcgc gggtttccgc cttgcgacgg agcggggtcg ctgccgaccg
    11341 gctcatcctc gatccgggga tgggattttt cttgagcccc gcaccggaaa catcgctgca
    11401 cgtgctgtcg aaccttcaaa agctgaagtc ggcgttgggg cttccgctat tggtctcggt
    11461 gtcgcggaaa tccttcttgg gcgccaccgt tggccttcct gtaaaggatc tgggtccagc
    11521 gagccttgcg gcggaacttc acgcgatcgg caatggcgct gactacgtcc gcacccacgc
    11581 gcctggagat ctgcgaagcg caatcacctt ctcggaaacc ctcgcgaaat ttcgcagtcg
    11641 cgacgccaga gaccgagggt tagatcatgc ctagcattca ccttccggcc gcccgctagc
    11701 ggaccctggt caggttccgc gaaggtgggc gcagacatgc tgggctcgtc aggatcaaac
    11761 tgcactatga ggcggcggtt cataccgcgc caggggagcg aatggacagc gaggagcctc
    11821 cgaacgttcg ggtcgcctgc tcgggtgata tcgacgaggt tgtgcggctg atgcacgacg
    11881 ctgcggcgtg gatgtccgcc aagggaacgc ccgcctggga cgtcgcgcgg atcgaccgga
    11941 cattcgcgga gaccttcgtc ctgagatccg agctcctagt cgcgagttgc agcgacggca
    12001 tcgtcggctg ttgcaccttg tcggccgagg atcccgagtt ctggcccgac gccctcaagg
    12061 gggaggccgc atatctgcac aagctcgcgg tgcgacggac acatgcgggc cggggtgtca
    12121 gctccgcgct gatcgaggct tgccgccatg ccgcgcgaac gcaggggtgc gccaagctgc
    12181 ggctcgactg ccacccgaac ctgcgtggcc tatacgagcg gctcggattc acccacgtcg
    12241 acactttcaa tcccggctgg gatccaacct tcatcgcaga acgcctagaa ctcgaaatct
    12301 aacgtccgtt cgggcatcga ggtccatgtc ggggtgggac gggcccgtgg cttcaagatc
    12361 acttgcagtc cgaccgcgat gtcttggttg cgcgagaggt tgtcgatatc ctccacttcc
    12421 atcatcaacc ctggataatg ccgccgccgt catcgccgcc gacgcccgtg ccgggctttt
    12481 cgggcctgtc aggcttgctc ggccttcagc ctgcctgggc gagatctccg gcggacggat
    12541 taacggcgga gcttcgccgc ctttcgtgcg tgtgaaggcc gaagatagtt ctctcaaaaa
    12601 catccgttta tgagagatac caaatgtcat tttcagaaga cgactgcacc agttgattgg
    12661 gcgtaatggc tgttgtgcag ccagctcctg acagttcaat atcagaagtg atctgcacca
    12721 atctcgacta tgctcaatac tcgtgtgggc tctgttgcaa aaatcgtgaa gcttgagcat
    12781 gcttggcgga gattggacgg acggaacgat gacggatttc aagtggcgcc atttccaggg
    12841 tgatgtgatc ctgtgggcgg tgcgctggta ttgtcgctat ccgatcagct atcgcgacct
    12901 tgaggaaatg ctggcggaac gcggcatttc ggtcgaccat acgacgatct atcgctgggt
    12961 ccagtgctac gccccggaga tggagaagcg gctgcgctgg ttctggcggc gtggctttga
    13021 tccgagctgg cgcctggatg aaacctacgt caaggtgcgg ggcaagtgga cctacctgta
    13081 ccgggcagtc gacaagcggg gcgacacgat cgatttctac ctgtcgccga cccgcagcgc
    13141 caaggcagcg aagcggttcc tgggcaaggc cctgcgaggc ctgaagcact gggaaaagcc
    13201 tgccacgctc aataccgaca aagcgccgag ctatggtgca gcgatcaccg aattgaagcg
    13261 cgaaggaaag ctggaccggg agacggccca ccggcaggtg aagtatctca ataacgtgat
    13321 cgaggccgat cacggaaagc tcaagatact gatcaagccg gtgcgcggtt tcaaatcgat
    13381 ccccacggcc tatgccacga tcaagggatt cgaagtcatg cgagccctgc gcaaaggaca
    13441 ggctcgcccc tggtgcctgc agcccggcat caggggcgag gtgcgccttg tggagagagc
    13501 ttttggcatt gggccctcgg cgctgacgga ggccatgggc atgctcaacc accatttcgc
    13561 agcagccgcc tgatcggcgc agagcgacag cctacctctg actgccgcca atctttgcaa
    13621 cagagcctcc gtcgccatgc tcacctcgct ttggtgcaca cgagtattga gcatagtcga
    13681 gattggtgca gatcacttct gatattgaac tgtcaggagc tggctgcaca acagccatta
    13741 cgcccaatca actggtgcag tcgtcttctg aaaatgacag tgctcctggt cggcgtcgga
    13801 gctggccggg tgggtgcggc ggaatttctc gacgatcatc gacttgccgt tgttggttgg
    13861 accgaccaac agcaggttgg gcatgcgttg tttgttcggc cacgcataca gggcttccag
    13921 ccgattcagc gcctcgactg ctcgcggata gccgatccag cggtcagcgc gaaggcgatg
    13981 gatgcgttcg tccgccggaa gacgggccaa gccctgggcc accggcatca ggtgggacaa
    14041 gtcgatgatg ggatattctt ccacggctac cactcctcaa tctggtcgaa cggtttggca
    14101 ggtggctggt tgtctgcctg cgggtcagcc atgtccgcgt ccggtggtgg cgtggttttg
    14161 aaaagaactg ccgttgcctt gagatgctgg cgtcgatccg cgtcgcgccg cgccttgcgt
    14221 gtagctttct gcgcggtgga cacgatttcg cgcatctggc cgatcatgcg aaacagcgcc
    14281 gactcatcca cctgttcgcg cccttgctgc cgcaatttcg ccagcgcctg tcgttgttcc
    14341 cagagggtga cagccgggtg cgacaaggtg cggtatggaa tttccagata gtgctgcccc
    14401 tccggctcca gcacccaaat gcggctgatg tcgcgcgggt cgcgccggat caggaacgca
    14461 ggcaagcggt cgcgccgagc tatccacggc ttgagcgcat cggcgtagta atggatgtgg
    14521 tcgatgacga agccggtgcg ggtcagcgtg cggcggatga tgggcagaaa atcgaccaga
    14581 aaagccgtgg tgcgagtgat gacggttggc acgccggtcc gcgcgatagc ttcggcccag
    14641 cgcgctgccg gcggctggag caggccgttg tgcacggagc cgtggtaggt gccgaccgcc
    14701 aatgtgagcc agcgctccag ctcacgcagc gtcagggcgg ccttgttttc ggaatcgtag
    14761 tcgccgcgct ggtcagggtt ggagaaggtc gtccctggca attcgtcgtg gatcatctgc
    14821 atcgccgtgc cgatgatccg ttccacgatg ccgccgtagt gcggctgccc gagcgggcga
    14881 tagtcaagcc ggatgccatg ctgctcgcag cctcggcgta gcgcttcgct cttgaactcg
    14941 gccgcgttgt ccaggtagag cagcctgggc ttgccgctca tcggccactc catttctatg
    15001 ttcagaccct ccagccaggg acgcttgtcg caggcgacat gcacaaggca caggccgacc
    15061 gaaacagatg acggcgcttc cagcgtgacg accatgccga gcacgcagcg ggtaaacacg
    15121 tcgatggcga tggtcagata cggacggcca atcggttgcc ggtcgcgctc gtccaccacg
    15181 atcaggtcga tgaccgtgtg atcaatctgc acttgttcca gtggcgcggt cacggcggga
    15241 ggctcaccac cgacaccttg caggctgcgg gacgcatcct gaccttcccg gcggcgagtg
    15301 gccttgagcg gatcgaggcc ggcgatccgc agagccacgg tgttgcgcgc cggcgcccgc
    15361 agcttttgcg ctttgcaagc ctgcgcgacc tcgcggtgga acgctgccag gctacgcttc
    15421 tgcttggtca ggaagcgctt ttgcagcaac tcgcggatga tgcgctcaac tgattccggc
    15481 aagcgtccct tgccttttcc gccgccggat cggctgcgag ccaggtccgt cacaagccca
    15541 gcaccttgcc gggcacggcg gattaggaca tacacctgcc gcctggacag gccaagcgcg
    15601 tgagcagcgg catcggcggc ttcatgcccg accacatcaa gcgctgccag cggcccgatg
    15661 atttccgccc gttgccgggc ctgcgcccaa gccgcatcgg gcagggtggc cacgccttgc
    15721 tcggcaatca atgatgtgtc tgacgccatg ctcacacctc gctttggtgc acacgagtat
    15781 tgagcatagt cgagattggt gcagatgact tctgatattg cgttgtcagg agtcgcctgc
    15841 acatctggcg ttacgccccg tcaattggtg cagtcgtctt ctgaaaatga ca
//
