LOCUS       In28                    6266 bp    DNA     linear   BCT 29-SEP-2020
DEFINITION  Escherichia coli.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Escherichia.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of In28
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     repeat_region   1..25
                     /gene="IRi_In28"
     CDS             complement(203..1216)
                     /gene="intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="Integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     misc_recomb     1297..1359
                     /gene="attI1"
     CDS             1422..2288
                     /gene="blaCARB-2"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase PSE-1/CARB-2"
                     /translation="MKFLLAFSLLIPSVVFASSSKFQQVEQDVKAIEVSLSARIGVSV
                     LDTQNGEYWDYNGNQRFPLTSTFKTIACAKLLYDAEQGKVNPNSTVEIKKADLVTYSP
                     VIEKQVGQAITLDDACFATMTTSDNTAANIILSAVGGPKGVTDFLRQIGDKETRLDRI
                     EPDLNEGKLGDLRDTTTPKAIASTLNKFLFGSALSEMNQKKLESWMVNNQVTGNLLRS
                     VLPAGWNIADRSGAGGFGARSITAVVWSEHQAPIIVSIYLAQTQASMAERNDAIVKIG
                     HSIFDVYTSQSR"
     misc_recomb     2292..2402
                     /gene="attC_blaCARB-2"
     CDS             2603..3862
                     /gene="cmlA1d"
                     /codon_start=1
                     /transl_table=11
                     /product="Chloramphenicol exporter CmlA1d"
                     /translation="MRSKNFSWRYSLAATVLLLSPFDLLASLGMDMYLPAVPFMPNAL
                     GTTASTIQLTLTTYLVMIGAGQLLFGPLSDRLGRRPVLLGGGLAYVVASMGLALTSSA
                     EVFLGLRILQACGASACLVSTFATVRDIYAGREESNVIYGILGSMLAIVPAVGPLLGA
                     LVDMWLGWRAIFAFLGLGMIAASAAAWRFWPETRVQRVAGLQWSQLLLPVKCLNFWLY
                     TLCYAAGMGSFFVFFSIAPGLMMGRQGVSQLGFSLLFATVAIAMVFTARFMGRVIPKW
                     GSPSVLRMGMGCLIAGAVLLAITEIWALQSVLGFIAPMWLVGIGVATAVSVAPNGALR
                     GFDHVAGTVTAVYFCLGGVLLGSIGTLIISLLPRNTAWPVVVYCLTLATVVLGLSCVS
                     RVKGSRGQGEHDVVALQSAESTSNPNR"
     misc_recomb     3882..3951
                     /gene="attC_cmlA1d"
     CDS             3955..4746
                     /gene="aadA1a"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3'-adenyltransferase AadA1a"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_recomb     4748..4807
                     /gene="attC_aadA1a"
     misc_feature    4910..5263
                     /gene="truncated qacED1"
                     /product="Truncated quaternary ammonium resistance
                     protein"
     repeat_region   5235..5248
                     /gene="IRL_IS6100"
     CDS             5296..6060
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6100 transposase"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     repeat_region   6101..6114
                     /gene="IRR_IS6100"
     repeat_region   6242..6266
                     /gene="IRt_In28"
ORIGIN      
        1 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
       61 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
      121 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
      181 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
      241 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
      301 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
      361 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
      421 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
      481 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
      541 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
      601 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
      661 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
      721 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
      781 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
      841 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
      901 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
      961 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     1021 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     1081 ggatgaaggc acgaacccag tggacataag cctgttctgt tcgtaagctg taatgcaagt
     1141 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1201 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat
     1261 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1321 ttacgcagca gggcagtcgc cctaaaacaa agttagccat attatggagc ctcatgcttt
     1381 tatataaaat gtgtgacaat caaaattatg gggttactta catgaagttt ttattggcat
     1441 tttcgctttt aataccatcc gtggtttttg caagtagttc aaagtttcag caagttgaac
     1501 aagacgttaa ggcaattgaa gtttctcttt ctgctcgtat aggtgtttcc gttcttgata
     1561 ctcaaaatgg agaatattgg gattacaatg gcaatcagcg cttcccgtta acaagtactt
     1621 ttaaaacaat agcttgcgct aaattactat atgatgctga gcaaggaaaa gttaatccca
     1681 atagtacagt cgagattaag aaagcagatc ttgtgaccta ttcccctgta atagaaaagc
     1741 aagtagggca ggcaatcaca ctcgatgatg cgtgcttcgc aactatgact acaagtgata
     1801 atactgcggc aaatatcatc ctaagtgctg taggtggccc caaaggcgtt actgattttt
     1861 taagacaaat tggggacaaa gagactcgtc tagaccgtat tgagcctgat ttaaatgaag
     1921 gtaagctcgg tgatttgagg gatacgacaa ctcctaaggc aatagccagt actttgaata
     1981 aatttttatt tggttccgcg ctatctgaaa tgaaccagaa aaaattagag tcttggatgg
     2041 tgaacaatca agtcactggt aatttactac gttcagtatt gccggcggga tggaacattg
     2101 cggatcgctc aggtgctggc ggatttggtg ctcggagtat tacagcagtt gtgtggagtg
     2161 agcatcaagc cccaattatt gtgagcatct atctagctca aacacaggct tcaatggcag
     2221 agcgaaatga tgcgattgtt aaaattggtc attcaatttt tgacgtttat acatcacagt
     2281 cgcgctgata aggctaacaa ggccatcaag ttgacggctt ttccgtcgct tgttttgtgg
     2341 tttaacgcta cgctaccaca aaacaatcaa ctccaaagcc gcaacttatg gcggcgttgg
     2401 gcgcacaata aggctccttg cagagttgct tgaaagttgt tacgattcaa atttaatcat
     2461 gagatagtca gcagatgagc acttccaaga acgcagacaa gtaagccgca gcaaccttca
     2521 tttttcggtt gttgcggcgt tctcatgaat ccttttgctc tacgggagcg ccgccaaatc
     2581 ctttgttcaa ggagatggtt tcgtgcgctc aaaaaacttt agttggcggt actcccttgc
     2641 cgccacggtg ttgttgttat caccgttcga tttattggca tcactcggca tggacatgta
     2701 cttgccagca gtgccgttta tgccaaacgc gcttggtacg acagcgagca caattcagct
     2761 tacgctgaca acgtacttgg tcatgattgg tgccggtcag ctcttgtttg gaccgctatc
     2821 ggaccgactg gggcgccgcc ccgttctact gggaggtggc ctcgcctacg ttgtggcgtc
     2881 aatgggcctc gctcttacgt catcggctga agtctttctg gggcttcgga ttcttcaggc
     2941 ttgtggtgcc tcggcgtgcc ttgtttccac atttgcaaca gtacgtgaca tttacgcagg
     3001 tcgcgaggaa agtaatgtca tttacggcat actcggatcc atgctggcca tagtcccggc
     3061 ggtaggccca ttgctcggag cgctcgtcga catgtggctt gggtggcggg ctatctttgc
     3121 gtttctaggt ttgggcatga tcgctgcatc tgcagcagcg tggcgattct ggcctgaaac
     3181 ccgggtgcaa cgagttgcgg gcttgcaatg gtcgcagctg ctactccccg ttaagtgcct
     3241 gaacttctgg ttgtacacgt tgtgttacgc cgctggaatg ggtagcttct tcgtcttttt
     3301 ctccattgcg cccggactaa tgatgggcag gcaaggtgtg tctcagcttg gcttcagcct
     3361 gctgttcgcc acagtggcaa ttgccatggt gtttacggct cgttttatgg ggcgtgtgat
     3421 acccaagtgg ggcagcccaa gtgtcttgcg aatgggaatg ggatgcctga tagctggagc
     3481 agtattgctt gccatcaccg aaatatgggc tttgcagtcc gtgttaggct ttattgctcc
     3541 aatgtggcta gtgggtattg gtgtcgccac agcggtatct gtggcgccca atggcgctct
     3601 tcgaggattc gaccatgttg ctggaacggt cacggcagtc tacttctgct tgggcggtgt
     3661 actgctagga agcatcggaa cgttgatcat ttcgctgttg ccgcgcaaca cggcttggcc
     3721 ggttgtcgtg tactgtttga cccttgcaac agtcgtgctc ggtctgtctt gtgtttcccg
     3781 agtgaagggc tctcgcggcc agggggagca tgatgtggtc gcgctacaaa gtgcggaaag
     3841 tacatcaaat cccaatcgtt gagagaatgt ggcaagctat cgcccaacaa atcgctgcag
     3901 ccgacccaaa accgctacgc ggtttcggtc ggctgagctc aggcgttaaa catcatgagg
     3961 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     4021 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     4081 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     4141 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     4201 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     4261 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     4321 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     4381 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     4441 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     4501 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     4561 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     4621 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcccgc
     4681 gcagatcagt tggaagaatt tgttcactac gtgaaaggcg agatcaccaa ggtagtcggc
     4741 aaataatgtc taacaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     4801 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     4861 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgaaaggctg
     4921 gctttttctt gttatcgcaa tagttggcga agtaatcgca acatccgcat taaaatctag
     4981 cgagggcttt actaagcttg ccccttccgc cgttgtcata atcggttatg gcatcgcatt
     5041 ttattttctt tctctggttc tgaaatccat ccctgtcggt gttgcttatg cagtctggtc
     5101 gggactcggc gtcgtcataa ttacagccat tgcctggttg cttcatgggc aaaagcttga
     5161 tgcgtggggc tttgtaggta tggggctcat aattgctgcc tttttgctcg cccgatcccc
     5221 atcgtggaag tcgcggctct gttgcaaaaa tcgtgaagct tgagcatgct tggcggagat
     5281 tggacggacg gaacgatgac ggatttcaag tggcgccatt tccagggtga tgtgatcctg
     5341 tgggcggtgc gctggtattg tcgctatccg atcagctatc gcgaccttga ggaaatgctg
     5401 gcggaacgcg gcatttcggt cgaccatacg acgatctatc gctgggtcca gtgctacgcc
     5461 ccggagatgg agaagcggct gcgctggttc tggcggcgtg gctttgatcc gagctggcgc
     5521 ctggatgaaa cctacgtcaa ggtgcggggc aagtggacct acctgtaccg ggcagtcgac
     5581 aagcggggcg acacgatcga tttctacctg tcgccgaccc gcagcgccaa ggcagcgaag
     5641 cggttcctgg gcaaggccct gcgaggcctg aagcactggg aaaagcctgc cacgctcaat
     5701 accgacaaag cgccgagcta tggtgcagcg atcaccgaat tgaagcgcga aggaaagctg
     5761 gaccgggaga cggcccaccg gcaggtgaag tatctcaata acgtgatcga ggccgatcac
     5821 ggaaagctca agatactgat caagccggtg cgcggtttca aatcgatccc cacggcctat
     5881 gccacgatca agggattcga agtcatgcga gccctgcgca aaggacaggc tcgcccctgg
     5941 tgcctgcagc ccggcatcag gggcgaggtg cgccttgtgg agagagcttt tggcattggg
     6001 ccctcggcgc tgacggaggc catgggcatg ctcaaccacc atttcgcagc agccgcctga
     6061 tcggcgcaga gcgacagcct acctctgact gccgccaatc tttgcaacag agcctccgtc
     6121 gccatgctca cctcgctttg gtgcacacga gtattgagca tagtcgagat tggtgcagat
     6181 cacttctgat attgaactgt caggagctgg ctgcacaaca gccattacgc ccaatcaact
     6241 ggtgcagtcg tcttctgaaa atgaca
//
