LOCUS       In2099                  5573 bp    DNA     linear       03-AUG-2023
DEFINITION  Pseudomonas aeruginosa strain NY8688 isolate NY8688.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Unclassified.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Zhou,D.
  TITLE     Compelete sequence of Tn6502
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="NY8688"
                     /isolate="NY8688"
                     /isolation_source="Beijing"
                     /host="Pseudomonas aeruginosa"
                     /country="China"
     repeat_region   complement(1..25)
                     /gene="IRt_In2099"
     misc_feature    142..573
                     /gene="ΔtniA"
                     /product="Truncated DD(35)E transposase TniA"
     misc_recomb     complement(576..696)
                     /gene="attC_blaIMP-1"
     CDS             complement(672..1412)
                     /gene="blaIMP-1"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase IMP-1"
                     /translation="MSKLSVFFIFLFCSIATAAESLPDLKIEKLDEGVYVHTSFEEVN
                     GWGVVPKHGLVVLVNAEAYLIDTPFTAKDTEKLVTWFVERGYKIKGSISSHFHSDSTG
                     GIEWLNSRSIPTYASELTNELLKKDGKVQATNSFSGVNYWLVKNKIEVFYPGPGHTPD
                     NVVVWLPERKILFGGCFIKPYGLGNLGDANIEAWPKSAKLLKSKYGKAKLVVPSHSEV
                     GDASLLKLTLEQAVKGLNESKKPSKPSN"
     misc_recomb     complement(1431..1561)
                     /gene="attC_aacA7"
     CDS             complement(1551..2009)
                     /gene="aacA7"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 6'-N-acetyltransferase AacA7"
                     /translation="MDSSPLVRPVETTDSASWLSMRCELWPDGTCQEHQSEIAEFLSG
                     KVARPAAVLIAVAPDGEALGFAELSIRPYAEECYSGNVAFLEGWYVVPSARRQGVGVA
                     LVKAAEHWARGRGCTEFASDTQLTNSASTSAHLAAGFTEVAQVRCFRKPL"
     misc_recomb     complement(2041..2100)
                     /gene="attC_aadB"
     CDS             complement(2068..2664)
                     /gene="aadB"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /translation="MLWSSNDVTQQGSRPKTKLGRMDTTQVTLIHKILAAADERNLPL
                     WIGGGWAIDARLGRVTRKHDDIDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIG
                     DELLDCEPAWWADEAYEIAEAPQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEV
                     PPVDWPTKHIESYRLACTSLGAEKVEVLRAAFRSRYAA"
     misc_recomb     complement(2604..2666)
                     /gene="attI1"
     CDS             2747..3760
                     /gene="intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="Integron integrase IntIPac"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     repeat_region   complement(3862..3886)
                     /gene="IRR_ISCfr1"
     CDS             complement(4027..5397)
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCfr1 transposase"
                     /translation="MMGQLPGGQQRLFYSFNLEDHVPAQHLLRSIDQCLDLSDLRAYL
                     ADFYSPIGRPSIDPELMVRMLVVGYCYGIRSERRLCEEVHLNLAYRWFCRLGLEDEVP
                     NHSTFSKNRHGRFRDSDLFRWLFNEVLRRCMAAGLVKGEGFAVDASIIKADASRQRGV
                     AGDEVDWNDPKLSSRAVREYLEALDEEALAEALPKKISLTDPQSRWTAAPGGPAFFAY
                     STNYLIDTEHGVIMDVEATPAHRTAEVDSTRTMVERVKAQFDLTPERLIGDTAYGTAP
                     MLAWMVEEKDIEPHVPVWDKTERKDDSLSSNDFHWNQEANEYRCPAGKPLRSEWRAFT
                     QKRSRVTKAHTIIYRSSQTDCTTCPLKAKCCPNTPNRKIVRSIHEAARDVARRIAKTP
                     EYLVSRCERKKVEMLFAHLKRIMKLDRLRLRGLTGATDEFTLAAAVQNLRRMAKLLPQ
                     GPPLTG"
     repeat_region   complement(5454..5478)
                     /gene="IRL_ISCfr1"
     repeat_region   complement(5549..5573)
                     /gene="IRi_In2099"
ORIGIN      
        1 tgtcattttc agaagacgac tgcaccagtt gattgggcgt aatggctgtt gtgcagccag
       61 ctcctgacag ttcaatatca gaagtgatct gcaccaatct cgactatgct caatactcgt
      121 gtgcaccaaa gcgaggtgag catggcgacg gacaccccac ggattccaga acaaggcgtg
      181 gccactctgc ctgatgaggc ttgggagcgt gcgcgccgtc gtgcggagat catcagtccg
      241 ttggcgcagt cggagacggt cgggcacgaa gcggccgata tggcggctca ggcgctgggc
      301 ttgtctcggc gccaggtata cgttctgatc cggcgtgccc ggcaaggcag cggcctcgtg
      361 acggatctgg tgcccggcca gtccggtgga ggtaaaggta aggggcgctt gccggaaccg
      421 gtcgagcgcg tcatccacga gctactgcaa aagcggttcc tgaccaagca gaagcgcagc
      481 ctagcggcct ttcaccgcga agtcactcag gtgtgcaagg ctcaaaaact gcgagtgccg
      541 gcgcgcaacg ctaagttcag cggcagtttg taagttgcgc gttgtggaat actttgcgaa
      601 gcaaaccaca aaagcgcaac ttacaaactg tccagccacg tagtggcgtg ctgcaacgac
      661 ttgttagaaa tttagttgct tggttttgat ggttttttac tttcgtttaa ccctttaacc
      721 gcctgctcta atgtaagttt caagagtgat gcgtctccaa cttcactgtg acttggaaca
      781 accagttttg ccttaccata tttggacttt aataatttgg cggactttgg ccaagcttct
      841 atatttgcgt cacccaaatt gcctaaaccg tacggtttaa taaaacaacc accgaataat
      901 attttccttt caggcagcca aaccactacg ttatctggag tgtgtcccgg gcctggataa
      961 aaaacttcaa ttttattttt aactagccaa tagttaactc cgctaaatga atttgtggct
     1021 tgaaccttgc cgtctttttt aagcagttca tttgttaatt cagatgcata cgtggggata
     1081 gatcgagaat taagccactc tattccgccc gtgctgtcgc tatgaaaatg agaggaaata
     1141 ctgcctttta ttttatagcc acgctccaca aaccaagtga ctaacttttc agtatcttta
     1201 gccgtaaatg gagtgtcaat tagataagcc tcagcattta caagaaccac caaaccatgt
     1261 ttaggaacaa cgccccaccc gttaacttct tcaaacgaag tatgaacata aacgccttca
     1321 tcaagctttt caatttttaa atctggcaaa gactctgctg cggtagcaat gctgcaaaac
     1381 aaaaatataa agaatacaga taacttgctc atacttttcc ttttctaacg cctgagttaa
     1441 gccgacttgc gtagtggagc caaagccatg gcaagctctt tctgccatgg ctttggtgta
     1501 acgaagcaag ttcggcttga acgaattgtt aggcaccaac gcggcgcccc tcacaacggt
     1561 ttccggaagc agcgtacttg agcaacctcc gtgaatccag ccgccaggtg cgccgaggtg
     1621 cttgcgctgt tggtaagttg agtgtcggag gcgaattcgg tgcatccgcg accacgagcc
     1681 caatgctcgg cggcttttac cagagctaca cctacgccct gacgccgcgc acttggcaca
     1741 acgtaccaac cctccaagaa cgcaacgttg ccggagtagc actcctccgc atacgggcgg
     1801 atcgaaagct cggcaaaccc tagtgcttct ccgtcgggtg ctacagcaat gaggacagca
     1861 gcaggccggg cgacttttcc ggacagaaat tctgcgatct ctgactggtg ctcttgacat
     1921 gtgccatctg gccacagctc acagcgcatg cttagccaac tggccgaatc ggtagtttca
     1981 acaggcctga cgagcggcga actatccatt ggtgcctaac acctgagtta agccgcgccg
     2041 cgaagcggcg tcggcttgga cgaattgtta ggccgcatat cgcgacctga aagcggcacg
     2101 caagacctca accttttccg ccccgagtga ggtgcatgcg agcctgtagg actctatgtg
     2161 ctttgtaggc cagtccactg gtggtacttc atcggcatag taaaagtaat cccagatgat
     2221 cgcctcccag ctgttacaac ggactggccg cccggcgatg acgccctcag ccgcctctgg
     2281 gcacgagccc tgcggagcct ccgcgatttc atacgcttcg tctgcccacc aagcaggttc
     2341 gcagtcaagt aactcatccc cgatctccgc taagaatcca tagtccaact cctccatgac
     2401 gcgcccgccg agcatttcaa ctattgcctc gagctcgccg cgcctctcgc cgggaaacgt
     2461 cagatcaata tcatcgtgct tgcgtgttac acgccctagc cgtgcatcga tcgcccagcc
     2521 cccaccgatc cagagcggca gatttcgctc atctgccgca gctagaattt tgtgtatcaa
     2581 tgtgacctgc gttgtgtcca tgcggcctaa ctttgtttta gggcgactgc cctgctgcgt
     2641 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     2701 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac
     2761 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg
     2821 ctacttgcat tatagcttac gaaccgaaca ggcttatgtc cactgggttc gtgccttcat
     2881 ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct
     2941 gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc
     3001 cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg
     3061 aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat
     3121 cctcggtttt ctggaaggcg agcatcgttt gttcgcccag cttctgtatg gaacgggcat
     3181 gcggatcagt gagggtttgc aactgcgggt caaggatctg gatttcgatc acggcacgat
     3241 catcgtgcgg gagggcaagg gctccaagga tcgggccttg atgttacccg agagcttggc
     3301 acccagcctg cgcgagcagc tgtcgcgtgc acgggcatgg tggctgaagg accaggccga
     3361 gggccgcagc ggcgttgcgc ttcccgacgc ccttgagcgg aagtatccgc gcgccgggca
     3421 ttcctggccg tggttctggg tttttgcgca gcacacgcat tcgaccgatc cacggagcgg
     3481 tgtcgtgcgt cgccatcaca tgtatgacca gacctttcag cgcgccttca aacgtgccgt
     3541 agaacaagca ggcatcacga agcccgccac accgcacacc ctccgccact cgttcgcgac
     3601 ggccttgctc cgcagcggtt acgacattcg aaccgtgcag gatctgctcg gccattccga
     3661 cgtctctacg acgatgattt acacgcatgt gctgaaagtt ggcggtgccg gagtgcgctc
     3721 accgcttgat gcgctgccgc ccctcactag tgagaggtag ggcagcgcaa gtcaatcctg
     3781 gcggattcac tacccctgcg cgaaggccat cggtgccgca tcgaacggcc ggttgcggaa
     3841 agtcctccct gcgtccgctg atggccgatt ctgttgaaaa agtaggttta gcggcggcct
     3901 gccgatcagg tgtgcctgct gtcgaagtga ctgcaagcca cttcaggttg cctttcggcg
     3961 tttcgctgag cgtccttgct caggcctgag ggttaatttg aaggtttctg ctctcagcag
     4021 gcgtacctat cccgtgagcg gtggcccttg aggcaaaagc ttggccatgc gtcgcaggtt
     4081 ctgcaccgcc gccgccaaag tgaattcgtc agtggcacct gtcaggccgc gcagtcgtaa
     4141 acgatcgagt ttcatgatcc gtttgaggtg ggcgaacagc atctccacct tctttcgttc
     4201 gcagcgagag acgaggtatt ccggtgtctt ggcgatgcgc ctggccacat cgcgggcagc
     4261 ctcatggata ctgcggacga tcttccgatt cggcgtgttg gggcagcatt tcgctttcag
     4321 cggacaggta gtgcagtcgg tttggctgga gcgataaatg atggtgtggg ccttggtcac
     4381 ccgcgatctt ttctgggtga aggcgcgcca ctcactgcgt agtggtttgc cggctgggca
     4441 gcggtattca ttggcctcct ggttccagtg gaagtcgtta ctggagaggc tgtcgtcctt
     4501 gcgctcagtc ttgtcccaca ccggcacatg cggctcgatg tccttttctt cgaccatcca
     4561 ggccagcatc ggggcggtgc cataagcggt atcgccgata aggcgttccg gtgtgagatc
     4621 gaactgagct ttgacacgct caaccatcgt cctagtcgaa tcgacttcgg cggtacggtg
     4681 cgccggggta gcttccacgt ccatgatcac accgtgctca gtgtcgatca ggtaattcgt
     4741 ggagtaggca aaaaaggccg gcccacctgg cgctgctgtc caacgggact gaggatctgt
     4801 gagcgaaatt ttcttgggaa gagcctcagc cagcgcctct tcatcaaggg cttcgaggta
     4861 ctcgcgcact gcgcggctgc tgagctttgg atcgttccaa tcgacctcat ctcccgctac
     4921 cccacgttgc cggctggcat ccgccttgat gatgctagcg tcgacggcaa aaccttcgcc
     4981 cttgactagg ccggctgcca tgcaacgccg cagcacctca ttgaacaacc agcggaacag
     5041 atcgctgtca cgaaaacgcc catggcgatt cttcgagaag gtcgagtgat tggggacttc
     5101 gtcttccaga cccagccggc agaaccagcg ataggccagg ttcaggtgca cctcttcgca
     5161 caatcgccgc tcggaacgaa tgccatagca gtagccgacg accagcatgc gcaccatcaa
     5221 ttccgggtca atcgaggggc gtccgatggg actatagaag tccgccaggt aggcgcgcag
     5281 atcactaaga tccaggcact ggtcgatgct gcgcaggagg tgttgagccg ggacgtgatc
     5341 ttctaggttg aacgagtaga acaggcgctg ctgtcctccc ggtaactgcc ccatcatgct
     5401 gttcgccccc acgctcgctg acagagcaat tttgccaacg gtatggagag gccgctactt
     5461 tttcaacaga atcggccggc agcagcccgt cgttgcctga tggatccaac ccctccgctg
     5521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg aca
//
