LOCUS       In2098                  6102 bp    DNA     linear       03-AUG-2023
DEFINITION  Pseudomonas aeruginosa strain NY8709 isolate NY8709.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Unclassified.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Zhou,D.
  TITLE     Compelete sequence of Tn6502
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="NY8709"
                     /isolate="NY8709"
                     /isolation_source="Beijing"
                     /host="Pseudomonas aeruginosa"
                     /country="China"
     repeat_region   1..25
                     /gene="IRi_In2098"
     CDS             complement(203..1216)
                     /gene="intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="Integrase IntI1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     misc_recomb     1297..1359
                     /gene="attI1"
     CDS             1369..2109
                     /gene="blaIMP-1"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase IMP-1"
                     /translation="MSKLSVFFIFLFCSIATAAESLPDLKIEKLDEGVYVHTSFEEVN
                     GWGVVPKHGLVVLVNAEAYLIDTPFTAKDTEKLVTWFVERGYKIKGSISSHFHSDSTG
                     GIEWLNSRSIPTYASELTNELLKKDGKVQATNSFSGVNYWLVKNKIEVFYPGPGHTPD
                     NVVVWLPERKILFGGCFIKPYGLGNLGDANIEAWPKSAKLLKSKYGKAKLVVPSHSEV
                     GDASLLKLTLEQAVKGLNESKKPSKPSN"
     misc_recomb     2112..2238
                     /gene="attC_blaIMP-1"
     CDS             2258..2812
                     /gene="aacA3"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 6'-N-acetyltransferase"
                     /translation="MSASTPPITLRLMTERDLPMLHDWLNRPHIVEWWGGDEERPTLD
                     EVLEHYLPRAMAEESVTPYIAMLGEEPIGYAQSYVALGSGDGWWEDETDPGVRGIDQS
                     LADPTQLNKGLGTRLVRALVELLFSDPTVTKIQTDPTPNNHRAIRCYEKAGFVREKII
                     TTPDGPAVYMVQTRQAFERKRGVA"
     misc_recomb     2807..2866
                     /gene="attC_aacA3"
     CDS             2969..3316
                     /gene="qacED1"
                     /codon_start=1
                     /transl_table=11
                     /product="Quarternary ammonium coumpound resistance
                     protein"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     CDS             3310..4149
                     /gene="sul1"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydropteroate synthase"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     CDS             4277..4777
                     /gene="orf5"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     CDS             4801..5088
                     /gene="orf6"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MSGWDGPVASRSLAVRPRCLGCARGCRYPPLPSSTLDNAAAVIA
                     ADARAGLFGPVRLARPSACLGEISGGRINGGASPPFVRVKAEDSSLKNIRL"
     repeat_region   5100..5124
                     /gene="IRt_In2098"
     repeat_region   5223..5236
                     /gene="IRL_IS6100"
     CDS             5284..6048
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6100 transposase TnpA"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     repeat_region   6089..6102
                     /gene="IRR_IS6100"
ORIGIN      
        1 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
       61 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
      121 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
      181 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
      241 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
      301 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
      361 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
      421 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
      481 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
      541 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
      601 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
      661 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
      721 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
      781 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
      841 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
      901 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
      961 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     1021 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     1081 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     1141 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1201 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat
     1261 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1321 ttacgcagca gggcagtcgc cctaaaacaa agttagaaaa ggaaaagtat gagcaagtta
     1381 tctgtattct ttatattttt gttttgcagc attgctaccg cagcagagtc tttgccagat
     1441 ttaaaaattg aaaagcttga tgaaggcgtt tatgttcata cttcgtttga agaagttaac
     1501 gggtggggcg ttgttcctaa acatggtttg gtggttcttg taaatgctga ggcttatcta
     1561 attgacactc catttacggc taaagatact gaaaagttag tcacttggtt tgtggagcgt
     1621 ggctataaaa taaaaggcag tatttcctct cattttcata gcgacagcac gggcggaata
     1681 gagtggctta attctcgatc tatccccacg tatgcatctg aattaacaaa tgaactgctt
     1741 aaaaaagacg gcaaggttca agccacaaat tcatttagcg gagttaacta ttggctagtt
     1801 aaaaataaaa ttgaagtttt ttatccaggc ccgggacaca ctccagataa cgtagtggtt
     1861 tggctgcctg aaaggaaaat attattcggt ggttgtttta ttaaaccgta cggtttaggc
     1921 aatttgggtg acgcaaatat agaagcttgg ccaaagtccg ccaaattatt aaagtccaaa
     1981 tatggtaagg caaaactggt tgttccaagt cacagtgaag ttggagacgc atcactcttg
     2041 aaacttacat tagagcaggc ggttaaaggg ttaaacgaaa gtaaaaaacc atcaaaacca
     2101 agcaactaaa tttctaacaa gtcgttgcag cacgccacta cgtggctgga cagtttgtaa
     2161 gttgcgcttt tgtggtttgc ttcgcaaagt attccacaac gcgcaactta caaactgccg
     2221 ctgaacttag cgttaggcag cacagagcga ccatttcatg tccgcgagca ccccccccat
     2281 aactcttcgc ctcatgaccg agcgcgacct gccgatgctc catgattggc tcaaccggcc
     2341 gcacatcgtt gagtggtggg gtggtgacga agagcgaccg actcttgatg aagtgctgga
     2401 acactacctg cccagagcga tggcggaaga gtccgtaaca ccgtacatcg caatgctggg
     2461 cgaggaaccg atcggctatg ctcagtcgta cgtcgcgctc ggaagcggtg atggctggtg
     2521 ggaagatgaa actgatccag gagtgcgagg aatagaccag tctctggctg acccgacaca
     2581 gttgaacaaa ggcctaggaa caaggcttgt ccgcgctctc gttgaactac tgttctcgga
     2641 ccccaccgtg acgaagattc agaccgaccc gactccgaac aaccatcgag ccatacgctg
     2701 ctatgagaag gcaggattcg tgcgggagaa gatcatcacc acgcctgacg ggccggcggt
     2761 ttacatggtt caaacacgac aagccttcga gagaaagcgc ggtgttgcct aacaactcat
     2821 tcaagccgac gccgcttcgc ggcgcggctt aattcaggtg ttagatgcac taagcacata
     2881 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     2941 aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg ttatcgcaat
     3001 agttggcgaa gtaatcgcaa catccgcatt aaaatctagc gagggcttta ctaagcttgc
     3061 cccttccgcc gttgtcataa tcggttatgg catcgcattt tattttcttt ctctggttct
     3121 gaaatccatc cctgtcggtg ttgcttatgc agtctggtcg ggactcggcg tcgtcataat
     3181 tacagccatt gcctggttgc ttcatgggca aaagcttgat gcgtggggct ttgtaggtat
     3241 ggggctcata attgctgcct ttttgctcgc ccgatcccca tcgtggaagt cgctgcggag
     3301 gccgacgcca tggtgacggt gttcggcatt ctgaatctca ccgaggactc cttcttcgat
     3361 gagagccggc ggctagaccc cgccggcgct gtcaccgcgg cgatcgaaat gctgcgagtc
     3421 ggatcagacg tcgtggatgt cggaccggcc gccagccatc cggacgcgag gcctgtatcg
     3481 ccggccgatg agatcagacg tattgcgccg ctcttagacg ccctgtccga tcagatgcac
     3541 cgtgtttcaa tcgacagctt ccaaccggaa acccagcgct atgcgctcaa gcgcggcgtg
     3601 ggctacctga acgatatcca aggatttcct gaccctgcgc tctatcccga tattgctgag
     3661 gcggactgca ggctggtggt tatgcactca gcgcagcggg atggcatcgc cacccgcacc
     3721 ggtcaccttc gacccgaaga cgcgctcgac gagattgtgc ggttcttcga ggcgcgggtt
     3781 tccgccttgc gacggagcgg ggtcgctgcc gaccggctca tcctcgatcc ggggatggga
     3841 tttttcttga gccccgcacc ggaaacatcg ctgcacgtgc tgtcgaacct tcaaaagctg
     3901 aagtcggcgt tggggcttcc gctattggtc tcggtgtcgc ggaaatcctt cttgggcgcc
     3961 accgttggcc ttcctgtaaa ggatctgggt ccagcgagcc ttgcggcgga acttcacgcg
     4021 atcggcaatg gcgctgacta cgtccgcacc cacgcgcctg gagatctgcg aagcgcaatc
     4081 accttctcgg aaaccctcgc gaaatttcgc agtcgcgacg ccagagaccg agggttagat
     4141 catgcctagc attcaccttc cggccgcccg ctagcggacc ctggtcaggt tccgcgaagg
     4201 tgggcgcaga catgctgggc tcgtcaggat caaactgcac tatgaggcgg cggttcatac
     4261 cgcgccaggg gagcgaatgg acagcgagga gcctccgaac gttcgggtcg cctgctcggg
     4321 tgatatcgac gaggttgtgc ggctgatgca cgacgctgcg gcgtggatgt ccgccaaggg
     4381 aacgcccgcc tgggacgtcg cgcggatcga ccggacattc gcggagacct tcgtcctgag
     4441 atccgagctc ctagtcgcga gttgcagcga cggcatcgtc ggctgttgca ccttgtcggc
     4501 cgaggatccc gagttctggc ccgacgccct caagggggag gccgcatatc tgcacaagct
     4561 cgcggtgcga cggacacatg cgggccgggg tgtcagctcc gcgctgatcg aggcttgccg
     4621 ccatgccgcg cgaacgcagg ggtgcgccaa gctgcggctc gactgccacc cgaacctgcg
     4681 tggcctatac gagcggctcg gattcaccca cgtcgacact ttcaatcccg gctgggatcc
     4741 aaccttcatc gcagaacgcc tagaactcga aatctaacgt ccgttcgggc atcgaggtcc
     4801 atgtcggggt gggacgggcc cgtggcttca agatcacttg cagtccgacc gcgatgtctt
     4861 ggttgcgcga gaggttgtcg atatcctcca cttccatcat caaccctgga taatgccgcc
     4921 gccgtcatcg ccgccgacgc ccgtgccggg cttttcgggc ctgtcaggct tgctcggcct
     4981 tcagcctgcc tgggcgagat ctccggcgga cggattaacg gcggagcttc gccgcctttc
     5041 gtgcgtgtga aggccgaaga tagttctctc aaaaacatcc gtttatgaga gataccaaat
     5101 gtcattttca gaagacgact gcaccagttg attgggcgta atggctgttg tgcagccagc
     5161 tcctgacagt tcaatatcag aagtgatctg caccaatctc gactatgctc aatactcgtg
     5221 tgggctctgt tgcaaaaatc gtgaagcttg agcatgcttg gcggagattg gacggacgga
     5281 acgatgacgg atttcaagtg gcgccatttc cagggtgatg tgatcctgtg ggcggtgcgc
     5341 tggtattgtc gctatccgat cagctatcgc gaccttgagg aaatgctggc ggaacgcggc
     5401 atttcggtcg accatacgac gatctatcgc tgggtccagt gctacgcccc ggagatggag
     5461 aagcggctgc gctggttctg gcggcgtggc tttgatccga gctggcgcct ggatgaaacc
     5521 tacgtcaagg tgcggggcaa gtggacctac ctgtaccggg cagtcgacaa gcggggcgac
     5581 acgatcgatt tctacctgtc gccgacccgc agcgccaagg cagcgaagcg gttcctgggc
     5641 aaggccctgc gaggcctgaa gcactgggaa aagcctgcca cgctcaatac cgacaaagcg
     5701 ccgagctatg gtgcagcgat caccgaattg aagcgcgaag gaaagctgga ccgggagacg
     5761 gcccaccggc aggtgaagta tctcaataac gtgatcgagg ccgatcacgg aaagctcaag
     5821 atactgatca agccggtgcg cggtttcaaa tcgatcccca cggcctatgc cacgatcaag
     5881 ggattcgaag tcatgcgagc cctgcgcaaa ggacaggctc gcccctggtg cctgcagccc
     5941 ggcatcaggg gcgaggtgcg ccttgtggag agagcttttg gcattgggcc ctcggcgctg
     6001 acggaggcca tgggcatgct caaccaccat ttcgcagcag ccgcctgatc ggcgcagagc
     6061 gacagcctac ctctgactgc cgccaatctt tgcaacagag cc
//
