LOCUS       In2091                  6919 bp    DNA     linear       03-AUG-2023
DEFINITION  Alcaligenes faecalis strain NY11312 isolate NY11312.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Alcaligenes faecalis
  ORGANISM  Alcaligenes faecalis
            Unclassified.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Zhou,D.
  TITLE     Compelete sequence of Tn6502
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Alcaligenes faecalis"
                     /mol_type="genomic DNA"
                     /strain="NY11312"
                     /isolate="NY11312"
                     /isolation_source="Beijing"
                     /host="Alcaligenes faecalis"
                     /country="China"
     repeat_region   complement(1..25)
                     /gene="IRt_In2091"
     CDS             142..1824
                     /gene="tniA"
                     /codon_start=1
                     /transl_table=11
                     /product="DD(35)E transposase TniA"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVVTLEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLV
                     DFLPILRRTLTRTGFVIDHIHYYADALKPWIARRERWPSFLIRRDPRDISRIWVLEPE
                     GQHYLEIPYRTLSHPAVTLWEQRQALMKLRQQGREQVDESALFRMIDQMRKIVTTAQK
                     TTRKARRDADRRQHLKATAPPVKATPPPDADMDDQQADNQPPAKPFDQIEEW"
     CDS             1827..2735
                     /gene="tniB"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein TniB"
                     /translation="MEEYPIIELSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVE
                     ALNRLETLYAWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHLASADADQEHIPVLVVQ
                     MPSEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRILVIDELHNVLAG
                     NSVNRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPLWEANDD
                     CCSLLASFAASLPLRRPSSIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESCEEA
                     INHRTLSMADYTGPSERRRQFERELM"
     CDS             2732..3949
                     /gene="tniQ"
                     /codon_start=1
                     /transl_table=11
                     /product="Transposition auxiliary protein TniQ"
                     /translation="MKPAPRWPLHPAPREGEALSSWLNRVALCYHMEVSELLEHDLGH
                     GQVDDLDTAPPLALLEMLSLRSGIEPDRLRCMSFAGWVPWLLDSLDDQIPDALETYAF
                     QLSVLLPKLRRRTRSITSWRAWLPSQPIHRACPLCLNDPANQAVLLAWKLPLMLSCPL
                     HGCWLESYWGVPGRFLGWENADTAPRTASDAIAAMDQRTWQALTTGHVELPRRRIHAG
                     LWFRLLRTLLDELNTPLSACGTYAGYLRQVWQGCGHPLRAGQSLWRPYETLNPAVRLQ
                     MLEAAAAAISLIEVRDISPPGEHAKLFWSEPQTGFTSGLPAKAPKPEPVNHWQRAVQA
                     ISEAIIEARHNPETARSLFALASYGRRDPASLEQLRATFAKEGIPPEFLSHYEPDGPF
                     ACLRQNDGLSDKF"
     misc_recomb     3973..4000
                     /gene="res"
     CDS             3996..4634
                     /gene="tniR"
                     /codon_start=1
                     /transl_table=11
                     /product="Serine resolvase TniR"
                     /translation="MTGEKVLIGYMRVSKADGSQSTNLQRDALIAAGVSLAHLYEDLA
                     SGRRDDRPGLAACLKALREGDTLIVWKLDRLGRDLRHLINTVHDLTARSVGLKVLTGH
                     GAAVDTTTAAGKLVFGIFAALAEFERELISERTVAGLISARARGRKGGRPFKMTAAKL
                     RLAMASMGQPETKVGDLCEELGITRQTLYRHVSPKGELRPDGVKLLSLGSAA"
     misc_recomb     complement(4731..4808)
                     /gene="attC_blaIMP-8"
     CDS             complement(4811..5551)
                     /gene="blaIMP-8"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase IMP-8"
                     /translation="MKKLFVLCVCFLCSITAAGAALPDLKIEKLEEGVYVHTSFEEVN
                     GWGVVSKHGLVVLVNTDAYLIDTPFTATDTEKLVNWFVERGYKIKGTISSHFHSDSTG
                     GIEWLNSQSIPTYASELTNELLKKDGKVQAKNSFSGVSYWLVKNKIEVFYPGPGHTQD
                     NVVVWLPEKKILFGGCFVKPDGLGNLGDANLEAWPKSAKILMSKYGKAKLVVSSHSEI
                     GDASLLKRTWEQAVKGLNESKKPSQPSN"
     misc_recomb     complement(5561..5623)
                     /gene="attI"
     CDS             5704..6717
                     /gene="intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="IntI1 integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVNWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     repeat_region   complement(6895..6919)
                     /gene="IRi_In2091"
ORIGIN      
        1 tgtcattttc agaagacgac tgcaccagtt gattgggcgt aatggctgtt gtgcagccag
       61 ctcctgacag ttcaatatca gaagtgatct gcaccaatct cgactatgct caatactcgt
      121 gtgcaccaaa gcgaggtgag catggcgacg gacaccccac ggattccaga acaaggcgtg
      181 gccactctgc ctgatgaggc ttgggagcgt gcgcgccgtc gtgcggagat catcagtccg
      241 ttggcgcagt cggagacggt cgggcacgaa gcggccgata tggcggctca ggcgctgggc
      301 ttgtctcggc gccaggtata cgttctgatc cggcgtgccc ggcaaggcag cggcctcgtg
      361 acggatctgg tgcccggcca gtccggtgga ggtaaaggta aggggcgctt gccggaaccg
      421 gtcgagcgcg tcatccacga gctactgcaa aagcggttcc tgaccaagca gaagcgcagc
      481 ctagcggcct ttcaccgcga agtcactcag gtgtgcaagg ctcaaaaact gcgagtgccg
      541 gcgcgcaata ccgtggcctt acggatcgct agccttgacc cgcgcaaggt catccgccgg
      601 cgggaaggcc aggatgccgc tcgtgaccta caaggtgtgg gcggcgagcc tcctgccgtg
      661 accgcgccgc tggagcaggt gcagatagac catacggtca tcgacctgat cgtggtcgat
      721 gaccgcgacc ggcaacctat tggccgcccg tacctgaccc tcgccatcga cgtgttcacc
      781 cgctgcgtgc tcggcatggt cgtcacgctg gaagcgccgt ctgccgtttc ggttggcctg
      841 tgcctcgtgc atgtcgcctg cgacaagcgc ccttggctgg aaggactgaa cgtggaaatg
      901 gattggcaga tgagcggcaa gcccttgctg ctctacctag acaacgcggc cgagttcaag
      961 agcgaggccc tgcgccgggg ttgcgagcag catggcatcc ggctggacta tcgcccgctg
     1021 ggacagccgc actatggcgg catcgtggaa cggatcatcg gcacggcgat gcagatgatt
     1081 cacgacgaac tgccgggaac gaccttctcc aaccctgacc agcgcggcga ctacgattcc
     1141 gaaaacaagg ccgccctgac gctgcgcgag ctagagcgct ggctcacatt ggcggtcggc
     1201 acctaccacg gttcggtgca caacggcctg ctccaaccgc cggccgcgcg ctgggccgag
     1261 gccgtggcgc gtgtcggcgt accggccgtc gtcacacgcg ctacttcgtt cctggtcgat
     1321 tttctgccga tcctccggcg cacgctgacc cgcaccggct ttgtcatcga ccacatccac
     1381 tactacgccg atgcgctcaa gccgtggatt gcgcggcgtg aacgctggcc gtcctttctg
     1441 atccggcgcg atccgcgcga catcagccgt atctgggtcc tggaaccgga gggacagcac
     1501 tacctggaaa ttccctaccg taccttgtcg cacccggctg tcaccctctg ggaacaacgg
     1561 caggcgctga tgaaattgcg gcagcaaggg cgcgaacagg tggatgagtc ggcgttgttc
     1621 cgcatgatcg accagatgcg caaaatcgtg accaccgcgc agaagaccac gcgcaaggcg
     1681 cggcgcgacg cagatcgacg ccagcatctc aaggcaacgg caccgcctgt caaagccacg
     1741 ccaccaccgg acgctgacat ggatgaccag caggcggaca accagccgcc cgccaaaccg
     1801 ttcgaccaga ttgaggagtg gtagccgtgg aagaatatcc catcatcgag ctgtcccacc
     1861 tgctgccggc ggcgcaggga ttggcccggc tgccggcgga cgagcgcatc caacgccttc
     1921 gcgccgaccg ctggatcggc tacccccgcg cggtcgaggc gctgaaccgg ctggaaaccc
     1981 tgtatgcgtg gccaaacaag caacgcatgc ccaacctgct gctggtcggc ccgaccaaca
     2041 acggcaagtc gatgatcgtc gagaagttcc ggcgcacgca tctggccagc gccgatgccg
     2101 accaggagca catcccggtg ctggtcgtgc agatgccgtc cgagccgtct gtgatccgct
     2161 tctacgtcgc gctgctcgcg gcgatgggtg cgccattgcg accgcgccca cgactgccgg
     2221 aaatggagca actggcgctg gcactgctgc gcaaggtcgg cgtgcgcatt ttggtgatcg
     2281 acgagttgca caacgtcctg gctggtaaca gcgtcaaccg ccgggaattc ctcaatctgc
     2341 tgcgcttcct cggcaacgaa ctgcgcatcc cactggtcgg ggttggcacg cgcgacgcct
     2401 atctggccat ccgctcggat gaccagttgg aaaaccgctt cgagccgatg atgctgccgc
     2461 tgtgggaggc caacgacgat tgctgctcac tgctggccag cttcgccgct tcgcttcctc
     2521 tgcggcgacc ctcgtcgatt gccacgctgg acatggcccg ctacctgctc acacgcagcg
     2581 agggcaccat aggcgaactg gcgcacttgc tgatggcagc ggccatcgtc gccgtggaga
     2641 gctgcgagga agcgatcaac catcgcacgc tcagcatggc cgattacacc ggtcccagcg
     2701 agcggcggcg gcaattcgag cgggaactga tgtgaagcca gcgccacgct ggccactgca
     2761 tccggctccc agggaaggcg aagccttgtc ttcgtggctc aaccgcgtgg ccctttgcta
     2821 tcacatggag gtgtccgagc tgctggagca cgatcttggt cacggtcagg ttgatgacct
     2881 ggacaccgcg ccaccactgg cgctgctgga gatgctctcc ctgcggagcg gcatcgagcc
     2941 ggatcggctg cgttgcatga gtttcgccgg ctgggtgcct tggctactgg acagtcttga
     3001 tgatcagatt ccagatgcat tggaaaccta tgcgttccag ctctcggtgc tgctgccgaa
     3061 actccgccgt aggacgcgat ccatcacgag ctggcgtgcc tggctgccca gccagccgat
     3121 acatcgcgcc tgtccgctct gtctgaacga cccggcaaac caagccgtac tgcttgcatg
     3181 gaagctgccc ttgatgctga gctgcccgct gcatggctgc tggctggaat cctattgggg
     3241 cgtgcctggg cggtttctcg gctgggagaa cgccgacact gcgccgcgca ccgccagcga
     3301 cgcgattgcg gcgatggacc agcgtacctg gcaggcactg acaaccggtc acgtggagct
     3361 gccgcgccga cgcatccacg ctggattgtg gtttcggctg ctacgcacgc tgctcgatga
     3421 gctgaacacc ccgctttcgg cgtgcggaac ctacgcgggg tatctccgcc aagtctggca
     3481 aggctgcggg catccgctgc gtgctgggca aagtctgtgg cgaccgtatg aaaccctgaa
     3541 cccggcagta cggttgcaga tgctggaggc agcggcagcg gcaatcagct tgattgaggt
     3601 gagggatata agcccgccag gcgagcatgc aaagctgttc tggtccgagc cccaaaccgg
     3661 gttcaccagt ggtctgccgg cgaaagcgcc gaagcccgaa cccgtcaatc actggcaacg
     3721 ggcggtccaa gctatcagtg aggcgatcat tgaagcgcgg cacaaccccg agacggcgcg
     3781 ctcgctgttc gccttggctt cctatggtcg gcgcgaccca gcttccctgg aacagttgcg
     3841 agccaccttt gcaaaggaag gcatcccccc ggaatttctg tcacattacg agcctgatgg
     3901 accctttgca tgtcttagac agaatgacgg gttaagtgac aaattttgac ggccagaatt
     3961 ttccggcgca cactgtcaca taatcgaacg tatacgtgac gggtgaaaag gtgctgatcg
     4021 gctacatgcg ggtatcgaag gcggacggat cccagtccac caatttgcaa cgcgatgcgc
     4081 tcatcgccgc tggtgtgagc cttgcgcacc tttacgagga tctggcctcg ggcaggcgcg
     4141 atgatcgccc agggttggct gcttgcctga aggcgcttcg tgaaggggac acgctgatcg
     4201 tgtggaagct cgatcggctt ggccgtgatc tgcgccacct gatcaacacc gtgcacgacc
     4261 taactgcgcg tagcgtgggc ctgaaggtcc tgaccggtca cggtgcggcg gtcgacacga
     4321 cgactgccgc cggcaagctt gtgttcggta tttttgccgc gctggccgag ttcgagcgtg
     4381 agttgatttc cgagcgaaca gtcgctggac ttatctcggc gcgcgctcgc ggcaggaaag
     4441 gggggcgccc cttcaagatg accgccgcca agctacgcct ggcgatggcc agcatggggc
     4501 aaccggaaac caaggtgggc gatctctgcg aagaactcgg gattacccgg cagacgctct
     4561 accggcacgt gtcgcccaag ggcgaactgc ggccagacgg cgtaaagctg ctctccctcg
     4621 gttcagccgc ataaatggag gcgacctgga acggggcgct gttcagtgcg gcaacgatcc
     4681 gattaccggt gtcgacccag agcagccgta gagcttttgg gaaagctgtc gttcaacgcg
     4741 aaggtaaacg gcgccgcttg cggcgtccag tgaagcgaag cggaatgagt ttcaccgcct
     4801 tgttagaaat ttagttactt ggctgtgatg gttttttact ttcatttagc cctttaacag
     4861 cctgttccca tgtacgtttc aagagtgatg cgtccccaat ttcactatga cttgaaacaa
     4921 ccagttttgc tttaccatat ttagacatta atattttggc ggactttggc caagcttcta
     4981 aatttgcgtc acccaaatta ccaagaccgt ccggtttaac aaaacaacca ccgaataaaa
     5041 ttttcttttc aggtaaccaa accactacgt tatcttgagt gtgccccggg ccgggataaa
     5101 aaacttcaat tttattttta actagccaat aactaactcc gctaaatgag tttttagctt
     5161 gcaccttacc gtctttttta agaagttcat ttgttaattc agatgcatac gtgggaatag
     5221 attgagaatt aagccactct attccccctg tgctgtcgct atggaaatgt gaggaaatag
     5281 tgcctttgat tttatagccg cgctccacaa accaattgac taacttttca gtatctgtag
     5341 cagtaaatgg agtgtcaatc agataggcgt cagtgtttac aagaaccacc aaaccgtgtt
     5401 tagaaacaac accccaaccg ttaacttctt cgaacgatgt atgaacataa acaccttctt
     5461 caagcttctc gatttttaaa tcaggcaaag ccgctcctgc ggcagtaatg ctacaaagga
     5521 agcatacaca taaaacaaat aatttcttca tacttgccct tttctaactt tgttttaggg
     5581 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
     5641 taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa
     5701 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
     5761 ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaac
     5821 tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggcagcagc
     5881 gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt ctccacgcat
     5941 cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac ggatctgccc
     6001 tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt gctgaccccg
     6061 gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccagctt
     6121 ctgtatggaa cgggcatgcg gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat
     6181 ttcgatcacg gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg
     6241 ttacccgaga gcttggcacc cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg
     6301 ctgaaggacc aggccgaggg ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag
     6361 tatccgcgcg ccgggcattc ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg
     6421 accgatccac ggagcggtgt cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc
     6481 gccttcaaac gtgccgtaga acaagcaggc atcacgaagc ccgccacacc gcacaccctc
     6541 cgccactcgt tcgcgacggc cttgctccgc agcggttacg acattcgaac cgtgcaggat
     6601 ctgctcggcc attccgacgt ctctacgacg atgatttaca cgcatgtgct gaaagttggc
     6661 ggtgccggag tgcgctcacc gcttgatgcg ctgccgcccc tcactagtga gaggtagggc
     6721 agcgcaagtc aatcctggcg gattcactac ccctgcgcga aggccatcgg tgccgcatcg
     6781 aacggccggt tgcggaaagt cctccctgcg tccgctgatg gccggcagca gcccgtcgtt
     6841 gcctgatgga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
     6901 ccgtcttctg aaaacgaca
//
