LOCUS       In2089                  6331 bp    DNA     linear       03-AUG-2023
DEFINITION  Pseudomonas sp. strain NY11382 isolate NY11382.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Pseudomonas sp.
  ORGANISM  Pseudomonas sp.
            Unclassified.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Zhou,D.
  TITLE     Compelete sequence of Tn6502
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /strain="NY11382"
                     /isolate="NY11382"
                     /isolation_source="Beijing"
                     /host="Pseudomonas sp."
                     /country="China"
     repeat_region   1..25
                     /gene="IRi_In2089"
     CDS             complement(203..1216)
                     /gene="IntI1"
                     /codon_start=1
                     /transl_table=11
                     /product="IntI1 integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     misc_recomb     1297..1359
                     /gene="attI1"
     CDS             1369..2109
                     /gene="blaIMP-34"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase IMP-34"
                     /translation="MSKLSVFFIFLFCSIATAAESLPDLKIEKLDEGVYVHTSFEEVN
                     GWGVVPKHGLVVLVNAEAYLIDTPFTAKDTEKLVTWFVERGYKIKGSISSHFHSDSTG
                     GIGWLNSRSIPTYASELTNELLKKDGKVQATNSFSGVNYWLVKNKIEVFYPGPGHTPD
                     NVVVWLPERKILFGGCFIKPYGLGNLGDANIEAWPKSAKLLKSKYGKAKLVVPSHSEV
                     GDASLLKLTLEQAVKGLNESKKPSKPSN"
     misc_recomb     2112..2238
                     /gene="attC_blaIMP-34"
     CDS             2242..3030
                     /gene="aadA5"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptomycin 3''-O-adenylyltransferase aadA5"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     misc_recomb     3077..3089
                     /gene="ΔattC_aadA5"
     CDS             3198..3545
                     /gene="qacED1"
                     /codon_start=1
                     /transl_table=11
                     /product="Quaternary ammonium compound resistance protein"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     CDS             3539..4378
                     /gene="sul1"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydropteroate synthase"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     CDS             4506..5006
                     /gene="orf5"
                     /codon_start=1
                     /transl_table=11
                     /product="Orf5 protein"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     CDS             5030..5317
                     /gene="orf6"
                     /codon_start=1
                     /transl_table=11
                     /product="Orf6 protein"
                     /translation="MSGWDGPVASRSLAVRPRCLGCARGCRYPPLPSSTLDNAAAVIA
                     ADARAGLFGPVRLARPSACLGEISGGRINGGASPPFVRVKAEDSSLKNIRL"
     repeat_region   5329..5353
                     /gene="IRt_In2089"
     repeat_region   5452..5465
                     /gene="IRL_IS6100"
     CDS             5513..6277
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6100 transposase"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     repeat_region   6318..6331
                     /gene="IRR_IS6100"
ORIGIN      
        1 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
       61 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
      121 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
      181 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
      241 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
      301 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
      361 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
      421 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
      481 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
      541 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
      601 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
      661 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
      721 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
      781 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
      841 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
      901 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
      961 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     1021 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     1081 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     1141 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1201 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat
     1261 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1321 ttacgcagca gggcagtcgc cctaaaacaa agttagaaaa ggaaaagtat gagcaagtta
     1381 tctgtattct ttatattttt gttttgcagc attgctaccg cagcagagtc tttgccagat
     1441 ttaaaaattg aaaagcttga tgaaggcgtt tatgttcata cttcgtttga agaagttaac
     1501 gggtggggcg ttgttcctaa acatggtttg gtggttcttg taaatgctga ggcttattta
     1561 attgacactc catttacggc taaagatact gaaaagttag tcacttggtt tgtggagcgt
     1621 ggctataaaa taaaaggcag tatttcctct cattttcata gcgacagcac gggcggaata
     1681 gggtggctta attctcgatc tatccccacg tatgcatctg aattaacaaa tgaactgctt
     1741 aaaaaagacg gtaaggttca agccacaaat tcatttagcg gagttaacta ttggctagtt
     1801 aaaaataaaa ttgaagtttt ttatccaggc ccgggacaca ctccagataa cgtagtggtt
     1861 tggctgcctg aaaggaaaat attattcggt ggttgtttta ttaaaccgta cggtttaggc
     1921 aatttgggtg acgcaaatat agaagcttgg ccaaagtccg ccaaattatt aaagtccaaa
     1981 tatggtaagg caaaactggt tgttccaagt cacagtgaag ttggagacgc atcactcttg
     2041 aaacttacat tagagcaggc ggttaaagga ttaaacgaaa gtaaaaaacc atcaaaacca
     2101 agcaactaaa tttctaacaa gtcgttgcag cacgccacta cgtggctgga cagtttgtag
     2161 gttgcgcttt tgtggtttgc ttcgcaaagt attccacaac gcgcaactta caaactgccg
     2221 ctgaacttag cgttaggcat catgggtgaa tttttccctg cacaagtttt caagcagctg
     2281 tcccacgctc gcgcggtgat cgagcgccat ctggctgcga cactggacac aatccacctg
     2341 ttcggatctg cgatcgatgg agggctgaag ccggacagcg acatagactt gctcgtgacc
     2401 gtcagcgccg cacctaacga ttcgctccgg caggcgctaa tgctcgattt gctgaaagtc
     2461 tcatcaccgc caggcgatgg cggaacatgg cgaccgctgg agctaactgt tgtcgctcga
     2521 agcgaagtag tgccttggcg ctatccggcg cggcgtgagc ttcagttcgg tgagtggctc
     2581 cgccacgaca tcctttccgg aacgttcgag cctgccgttc tggatcacga tcttgcgatt
     2641 ttgctgacca aggcgaggca acacagcctt gcgcttctag gcccatccgc agccacgttt
     2701 ttcgagccgg tgccgaagga gcatttctcc aaggcgcttt tcgacactat tgcccagtgg
     2761 aatgcagagt cggattggaa gggtgacgag cggaacgtcg ttcttgctct tgctcgcatt
     2821 tggtacagcg cttcaactgg tctcattgct cctaaggacg ttgctgccgc atgggtatcg
     2881 gagcgtttgc ctgccgagca tcggcccctc atctgcaagg cacgcgcggc gtacctgggt
     2941 agcgaggacg acgacctagc aatgcgcgtc gaagagacgg ccgcgttcgt tcgatatgcc
     3001 aaagcaacga ttgagagaat cttgcgttga gcggcatgtg cgaaaagtgc atcgacccgc
     3061 gccgagggca tctgatgcct aactatgcgt tagatgcact aagcacataa ttgctcacag
     3121 ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac
     3181 aaattgggag atatatcatg aaaggctggc tttttcttgt tatcgcaata gttggcgaag
     3241 taatcgcaac atccgcatta aaatctagcg agggctttac taagcttgcc ccttccgccg
     3301 ttgtcataat cggttatggc atcgcatttt attttctttc tctggttctg aaatccatcc
     3361 ctgtcggtgt tgcttatgca gtctggtcgg gactcggcgt cgtcataatt acagccattg
     3421 cctggttgct tcatgggcaa aagcttgatg cgtggggctt tgtaggtatg gggctcataa
     3481 ttgctgcctt tttgctcgcc cgatccccat cgtggaagtc gctgcggagg ccgacgccat
     3541 ggtgacggtg ttcggcattc tgaatctcac cgaggactcc ttcttcgatg agagccggcg
     3601 gctagacccc gccggcgctg tcaccgcggc gatcgaaatg ctgcgagtcg gatcagacgt
     3661 cgtggatgtc ggaccggccg ccagccatcc ggacgcgagg cctgtatcgc cggccgatga
     3721 gatcagacgt attgcgccgc tcttagacgc cctgtccgat cagatgcacc gtgtttcaat
     3781 cgacagcttc caaccggaaa cccagcgcta tgcgctcaag cgcggcgtgg gctacctgaa
     3841 cgatatccaa ggatttcctg accctgcgct ctatcccgat attgctgagg cggactgcag
     3901 gctggtggtt atgcactcag cgcagcggga tggcatcgcc acccgcaccg gtcaccttcg
     3961 acccgaagac gcgctcgacg agattgtgcg gttcttcgag gcgcgggttt ccgccttgcg
     4021 acggagcggg gtcgctgccg accggctcat cctcgatccg gggatgggat ttttcttgag
     4081 ccccgcaccg gaaacatcgc tgcacgtgct gtcgaacctt caaaagctga agtcggcgtt
     4141 ggggcttccg ctattggtct cggtgtcgcg gaaatccttc ttgggcgcca ccgttggcct
     4201 tcctgtaaag gatctgggtc cagcgagcct tgcggcggaa cttcacgcga tcggcaatgg
     4261 cgctgactac gtccgcaccc acgcgcctgg agatctgcga agcgcaatca ccttctcgga
     4321 aaccctcgcg aaatttcgca gtcgcgacgc cagagaccga gggttagatc atgcctagca
     4381 ttcaccttcc ggccgcccgc tagcggaccc tggtcaggtt ccgcgaaggt gggcgcagac
     4441 atgctgggct cgtcaggatc aaactgcact atgaggcggc ggttcatacc gcgccagggg
     4501 agcgaatgga cagcgaggag cctccgaacg ttcgggtcgc ctgctcgggt gatatcgacg
     4561 aggttgtgcg gctgatgcac gacgctgcgg cgtggatgtc cgccaaggga acgcccgcct
     4621 gggacgtcgc gcggatcgac cggacattcg cggagacctt cgtcctgaga tccgagctcc
     4681 tagtcgcgag ttgcagcgac ggcatcgtcg gctgttgcac cttgtcggcc gaggatcccg
     4741 agttctggcc cgacgccctc aagggggagg ccgcatatct gcacaagctc gcggtgcgac
     4801 ggacacatgc gggccggggt gtcagctccg cgctgatcga ggcttgccgc catgccgcgc
     4861 gaacgcaggg gtgcgccaag ctgcggctcg actgccaccc gaacctgcgt ggcctatacg
     4921 agcggctcgg attcacccac gtcgacactt tcaatcccgg ctgggatcca accttcatcg
     4981 cagaacgcct agaactcgaa atctaacgtc cgttcgggca tcgaggtcca tgtcggggtg
     5041 ggacgggccc gtggcttcaa gatcacttgc agtccgaccg cgatgtcttg gttgcgcgag
     5101 aggttgtcga tatcctccac ttccatcatc aaccctggat aatgccgccg ccgtcatcgc
     5161 cgccgacgcc cgtgccgggc ttttcgggcc tgtcaggctt gctcggcctt cagcctgcct
     5221 gggcgagatc tccggcggac ggattaacgg cggagcttcg ccgcctttcg tgcgtgtgaa
     5281 ggccgaagat agttctctca aaaacatccg tttatgagag ataccaaatg tcattttcag
     5341 aagacgactg caccagttga ttgggcgtaa tggctgttgt gcagccagct cctgacagtt
     5401 caatatcaga agtgatctgc accaatctcg actatgctca atactcgtgt gggctctgtt
     5461 gcaaaaatcg tgaagcttga gcatgcttgg cggagattgg acggacggaa cgatgacgga
     5521 tttcaagtgg cgccatttcc agggtgatgt gatcctgtgg gcggtgcgct ggtattgtcg
     5581 ctatccgatc agctatcgcg accttgagga aatgctggcg gaacgcggca tttcggtcga
     5641 ccatacgacg atctatcgct gggtccagtg ctacgccccg gagatggaga agcggctgcg
     5701 ctggttctgg cggcgtggct ttgatccgag ctggcgcctg gatgaaacct acgtcaaggt
     5761 gcggggcaag tggacctacc tgtaccgggc agtcgacaag cggggcgaca cgatcgattt
     5821 ctacctgtcg ccgacccgca gcgccaaggc agcgaagcgg ttcctgggca aggccctgcg
     5881 aggcctgaag cactgggaaa agcctgccac gctcaatacc gacaaagcgc cgagctatgg
     5941 tgcagcgatc accgaattga agcgcgaagg aaagctggac cgggagacgg cccaccggca
     6001 ggtgaagtat ctcaataacg tgatcgaggc cgatcacgga aagctcaaga tactgatcaa
     6061 gccggtgcgc ggtttcaaat cgatccccac ggcctatgcc acgatcaagg gattcgaagt
     6121 catgcgagcc ctgcgcaaag gacaggctcg cccctggtgc ctgcagcccg gcatcagggg
     6181 cgaggtgcgc cttgtggaga gagcttttgg cattgggccc tcggcgctga cggaggccat
     6241 gggcatgctc aaccaccatt tcgcagcagc cgcctgatcg gcgcagagcg acagcctacc
     6301 tctgactgcc gccaatcttt gcaacagagc c
//
