LOCUS       In207                  16983 bp    DNA     linear   BCT 26-OCT-2020
DEFINITION  Klebsiella pneumoniae.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Klebsiella; Klebsiella
            pneumoniae.
REFERENCE   1  (bases 1 to 16983)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of In207
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16983)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16983
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
     mobile_element  1..16983
                     /gene="In207"
                     /product="Complex class 1 integron: In207"
     repeat_region   1..25
                     /gene="IRi_In207"
                     /product="Inverted repeat at the integrase end of In207"
     CDS             complement(203..1216)
                     /gene="intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="Integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     regulatory      1220..1245
                     /gene="PcS"
                     /product="Promoter PcS"
     regulatory      1220..1225
                     /gene="-35 region_PcS"
                     /product="-35 region of PcS"
     regulatory      1240..1245
                     /gene="-10 region_PcS"
                     /product="-10 region of PcS"
     misc_recomb     1297..1359
                     /gene="attI1"
                     /product="attI1 site"
     CDS             1387..1845
                     /gene="dfrA25"
                     /codon_start=1
                     /transl_table=11
                     /product="Trimethoprim resistance"
                     /translation="MAARAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVGRKTFE
                     SMGPLPNRKYAVVTRSNWTAANENVVVFPSIDEAMGRLGEITDHVIVAGGGEIYHETI
                     PMASTLHVSTIDVEPEGDVFFPNIPGKFDVVFEQQFTSNINYCYQIWQKG"
     misc_recomb     1840..1926
                     /gene="attC_dfrA25"
                     /product="attC site of dfrA25"
     CDS             2029..2376
                     /gene="qacED1"
                     /codon_start=1
                     /transl_table=11
                     /product="Quaternary ammonium compound resistance protein"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     CDS             2283..3209
                     /gene="sul1"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydropteroate synthase"
                     /translation="MGLCRYGAHNCCLFARPIPIVEVAAEADAMVTVFGILNLTEDSF
                     FDESRRLDPAGAVTAAIEMLRVGSDVVDVGPAASHPDARPVSPADEIRRIAPLLDALS
                     DQMHRVSIDSFQPETQRYALKRGVGYLNDIQGFPDPALYPDIAEADCRLVVMHSAQRD
                     GIATRTGHLRPEDALDEIVRFFEARVSALRRSGVAADRLILDPGMGFFLSPAPETSLH
                     VLSNLQKLKSALGLPLLVSVSRKSFLGATVGLPVKDLGPASLAAELHAIGNGADYVRT
                     HAPGDLRSAITFSETLAKFRSRDARDRGLDHA"
     mobile_element  complement(3234..5387)
                     /gene="ISCR1"
                     /product="Insertion sequence: ISCR1"
     CDS             3614..5155
                     /gene="tnpA_ISCR1"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCR1 transposase"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASL
                     EAQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGAR
                     RMAESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIK
                     KAGYTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYD
                     ELNTLAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGP
                     QQGRKVFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPA
                     VSEKRLALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVF
                     APNSKHRVQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVC
                     EHCGGHVKVIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQ
                     SRLFD"
     CDS             complement(5277..5927)
                     /gene="sapA"
                     /codon_start=1
                     /transl_table=11
                     /product="Peptide transport periplasmic protein SapA"
                     /translation="MRLVLSSLIVMAGFLSGQAAAAPEQAPHADIRDSGFVYCVSGQV
                     NTFNPQKTSSGLIVDTLAAQLYDRLLDVDPYTYRLVPELAESWEVLDNGATYRFHLRR
                     DVQFQKTAWFTPTRKLNADDVVFTFERIFDRHHPWHNVNGSSFPYFDSLQFADNVQSV
                     RKLDNNTVEFRLTQPDASFMGYRKYKPPFCSSSEVSYLKFPHSFTAQAPILTAGPA"
     misc_recomb     5367..5387
                     /gene="oriIS"
                     /product="oriIS of ISCR1"
     CDS             6453..6914
                     /gene="orf462"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MNCMNKLLSLVVSLALLPGAVFAASTPDCVKVNKAQIEALFDKW
                     NESLKTGNAQTVSENYLSDAVLLPTVSNKARLTDAERVDYFEHFLAKKPTGKIDMRTI
                     RLGCNKAIDTGTYTFTFADKSTVSARYTFTYAWDGKEWKISTHHSSAMPEG"
     misc_feature    complement(6956..7484)
                     /gene="truncated qnrB2"
                     /product="Truncated quinolone resistance protein
                     (pseudogene)"
     mobile_element  complement(7485..8304)
                     /gene="IS26"
                     /product="Insertion sequence: IS26"
     repeat_region   complement(7485..7498)
                     /gene="IRR_IS26"
                     /product="IS26 inverted repeat right"
     CDS             complement(7537..8241)
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS26 transposase"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     repeat_region   complement(8291..8304)
                     /gene="IRL_IS26"
                     /product="IS26 inverted repeat left"
     mobile_element  8305..14770
                     /gene="truncated Tn6296"
                     /product="Unit transposon: truncated Tn6296"
     mobile_element  8305..9286
                     /gene="truncated Tn1722"
                     /product="Unit transposon: truncated Tn1722"
     misc_feature    complement(8305..8330)
                     /gene="truncated tnpA"
                     /product="Truncated Tn1722 transposase (pseudogene)"
     CDS             complement(8334..8894)
                     /gene="tnpR"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn1722 resolvase"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     misc_recomb     8961..9080
                     /gene="res"
                     /product="Resolution site"
     repeat_region   9249..9286
                     /gene="IRR-1_Tn1722"
                     /product="Tn1722 inverted repeat right"
     misc_feature    9343..9903
                     /gene="truncated repB"
                     /product="Truncated replication protein"
     CDS             10058..10453
                     /gene="orf396"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MANLPGTGSSSTGWPSSDLDARRKSTRWPTYQAPAAARPGGQVQ
                     IWTPEGNQPGGQLTRHRQQLDQVAHFVRTKSGDAMRNLGGNPPRLRDFFNVERAFSCH
                     SRPFWPIILIKKTGHPPRSIPENSDDPHS"
     CDS             10437..10715
                     /gene="orf279"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MIHTANRTFHQLYREWIRERREHMHNVLTWERDRYGARLVGLFY
                     RYCKVANPFPRCTLNTRINYRAHAVNLPDWPARSLELNKMWLSWREKK"
     CDS             10826..11251
                     /gene="klcA"
                     /codon_start=1
                     /transl_table=11
                     /product="Antirestriction protein KlcA"
                     /translation="MMQTELNPLICSLVATPRRMAAMPRYVGRFYVVFESMLYQQMKG
                     LCREYRGAYWLMWELSNGGFYMAPGRRDEMLNIEAMNYFSGQMSADAAGITACLYLYS
                     HLSFHTEGADQERFSRLYHSLRDWACEHDEKEAILAAID"
     CDS             11580..11876
                     /gene="korC"
                     /codon_start=1
                     /transl_table=11
                     /product="Transcriptional repressor protein KorC"
                     /translation="MIRPETLRPFAEDWQAPTADEIKEVLELIRQRKGLSKPLSGVDV
                     ADLVGLPGERGSGKGTRTFRRWVSKTNPSPIAYGAWSILAHLAGFGAIWDADRD"
     misc_feature    11881..12861
                     /gene="truncated tnpA"
                     /product="Truncated ISKpn6 transposase"
     mobile_element  11997..13029
                     /gene="truncated ISKpn6"
                     /product="Insertion sequence: truncated ISKpn6"
     repeat_region   13014..13029
                     /gene="IRR_ISKpn6"
                     /product="ISKpn6 inverted repeat right"
     CDS             complement(13111..13992)
                     /gene="blaKPC-2"
                     /codon_start=1
                     /transl_table=11
                     /product="Carbapenemase KPC-2"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSI
                     GVYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALV
                     PWSPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFR
                     LDRWELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNH
                     RIRAAVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAV
                     IAAAARLALEGLGVNGQ"
     misc_feature    complement(14273..14770)
                     /gene="truncated tnpA"
                     /product="Truncated ISKpn27 transposase (pseudogene)"
     mobile_element  complement(14273..14770)
                     /gene="truncated ISKpn27"
                     /product="Insertion sequence: truncated ISKpn27"
     repeat_region   complement(14273..14301)
                     /gene="IRR_ISKpn27"
                     /product="ISKpn27 inverted repeat right"
     mobile_element  complement(14771..15590)
                     /gene="IS26"
                     /product="Insertion sequence: IS26"
     repeat_region   complement(14771..14784)
                     /gene="IRR_IS26"
                     /product="IS26 inverted repeat right"
     CDS             complement(14823..15527)
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS26 transposase"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     misc_feature    complement(15574..16878)
                     /gene="truncated tniA"
                     /product="Truncated DD(35)E transposase TniA (pseudogene)"
     repeat_region   complement(15577..15590)
                     /gene="IRL_IS26"
                     /product="IS26 inverted repeat left"
     repeat_region   16959..16983
                     /gene="IRt_In207"
                     /product="Inverted repeat at the tni end of In207"
BASE COUNT     3867 a   4646 c   4715 g   3755 t
ORIGIN
        1 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
       61 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
      121 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
      181 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
      241 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
      301 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
      361 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
      421 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
      481 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
      541 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
      601 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
      661 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
      721 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
      781 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
      841 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
      901 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
      961 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     1021 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     1081 ggatgaaggc acgaacccag tggacataag cctgttcggt tggtaagctg taatgcaagt
     1141 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1201 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat
     1261 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1321 ttacgcagca gggcagtcgc cctaaaacaa agttaaccca ggacgagtac cttgaaagta
     1381 tcattgatgg ctgcaagagc gaaaaatggc gtaatcggtt gcggtcctga cattccttgg
     1441 tctgccaaag gggaacagct tcttttcaaa gcactgacct ataaccaatg gcttttggta
     1501 gggcgcaaaa catttgagtc tatggggccg ctgcccaata ggaaatacgc ggttgttacc
     1561 cgctcaaact ggacagcggc taatgaaaac gtagtggttt tcccgtcgat tgacgaagcg
     1621 atgggtagat taggcgagat cactgaccat gtcatcgtcg ccggtggtgg agaaatctac
     1681 catgaaacga tacccatggc ctctactctg catgtgtcga caatcgacgt tgagccagag
     1741 ggagacgttt tctttccgaa cattcctggg aagtttgatg tcgtttttga gcaacaattt
     1801 acatcaaaca ttaactattg ctatcaaatc tggcaaaagg gttaacaaag ctatgcaatt
     1861 gacggcaaaa agcttcgttc gcttcactca ctacgcaatt tgccgcaatt gatagcggcg
     1921 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat
     1981 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg
     2041 ctttttcttg ttatcgcaat agttggcgaa gtaatcgcaa catccgcatt aaaatctagc
     2101 gagggcttta ctaagcttgc cccttccgcc gttgtcataa tcggttatgg catcgcattt
     2161 tattttcttt ctctggttct gaaatccatc cctgtcggtg ttgcttatgc agtctggtcg
     2221 ggactcggcg tcgtcataat tacagccatt gcctggttgc ttcatgggca aaagcttgat
     2281 gcgtggggct ttgtaggtat ggggctcata attgctgcct ttttgctcgc ccgatcccca
     2341 tcgtggaagt cgctgcggag gccgacgcca tggtgacggt gttcggcatt ctgaatctca
     2401 ccgaggactc cttcttcgat gagagccggc ggctagaccc cgccggcgct gtcaccgcgg
     2461 cgatcgaaat gctgcgagtc ggatcagacg tcgtggatgt cggaccggcc gccagccatc
     2521 cggacgcgag gcctgtatcg ccggccgatg agatcagacg tattgcgccg ctcttagacg
     2581 ccctgtccga tcagatgcac cgtgtttcaa tcgacagctt ccaaccggaa acccagcgct
     2641 atgcgctcaa gcgcggcgtg ggctacctga acgatatcca aggatttcct gaccctgcgc
     2701 tctatcccga tattgctgag gcggactgca ggctggtggt tatgcactca gcgcagcggg
     2761 atggcatcgc cacccgcacc ggtcaccttc gacccgaaga cgcgctcgac gagattgtgc
     2821 ggttcttcga ggcgcgggtt tccgccttgc gacggagcgg ggtcgctgcc gaccggctca
     2881 tcctcgatcc ggggatggga tttttcttga gccccgcacc ggaaacatcg ctgcacgtgc
     2941 tgtcgaacct tcaaaagctg aagtcggcgt tggggcttcc gctattggtc tcggtgtcgc
     3001 ggaaatcctt cttgggcgcc accgttggcc ttcctgtaaa ggatctgggt ccagcgagcc
     3061 ttgcggcgga acttcacgcg atcggcaatg gcgctgacta cgtccgcacc cacgcgcctg
     3121 gagatctgcg aagcgcaatc accttctcgg aaaccctcgc gaaatttcgc agtcgcgacg
     3181 ccagagaccg agggttagat catgcctagc attcaccttc cggccgcccg ctaaatatct
     3241 ccttttgggt tgttaataaa acatccaata agttgactgt gcgtgaaaaa gaaagttttg
     3301 tgtgatggcg ttgaagatcg caccgttaag ctcttatgtg ggatggtgca gagctcgacg
     3361 actaccgata aaacgcaacc gccgcaaaca gacaagaaaa agccccaact gataacagtt
     3421 ggggcttcag tattgtgatt ggtggagcaa tagcaccctg aacccaaaac cttctcgctc
     3481 aaccggtagt ggctgataac aactcgtgag ggctattgcg ggttaagcat ttagcgatgt
     3541 ctagggccag actggacgtc tgaacgcaag ccgctgatac tgtacataac cacagtatca
     3601 gcggaggata cccatgtcgc tggcaaggaa cgccacggcg agtcaatcgc ccactcaaac
     3661 aaacggttac gaacgccacc aacccgacca gacgctgctc taccagctgg ttgagcagca
     3721 ctacccagcc ttcaaagcct cactcgaagc ccaaggtcaa cacctgcctc gctacatcca
     3781 acaagaattc aacgacctcc tccaatgtgg ccgtctggag tatggtttca tgcgggttcg
     3841 ctgcgaggat tgtcatcacg agcgtctggt cgccttcagc tgtaaacgac gcggcttttg
     3901 ccctagctgc ggtgcccgcc ggatggccga gagtgcggcg ctgctgatag acgaagtctt
     3961 ccccaaggag cccattcgcc agtgggtgct cagctttcct ttccagctac gctttttgct
     4021 ggctcgccat ccccagctga tgggccaggt cttgagtatc gtctatcgta cactctcaac
     4081 tcatctgatc aaaaaagccg gttacaccaa agcctctgca caaactggct cagtgactct
     4141 tatccaacgc tttggctccg cgctaaatct caatgtccac taccacatgc tgtttctcga
     4201 tggtgtctat gccgaagatg actatggcaa gcaacgcttc catcgtgtca aggcacccac
     4261 ttacgatgag ctgaatacgc tcgctcacac cctcagccat cgcatcgctc gctgcatgga
     4321 aaagcgtggg attttggagc gtgatgccga gaatacgtgg ttgacactgg aagagggcga
     4381 agacgatacg ctgactcaat tacatggtgc ttcggttacg tatcgcattg ccgtcggccc
     4441 ccagcaaggg cgcaaagtct tcaccctgca aaccttgcca gggcgtgagg ataaagccga
     4501 ctcaagcagt cgagtagcca accatgctgg tttctcgcta cacgccggtg tgatggccga
     4561 agcgcatcag cgggataagc ttgagcgctt gtgtcgctac attagtcggc cagcggtttc
     4621 agaaaaacgt ctggcattaa ccgccaatgg gcaggtgcgt tacgagctca aaactccgta
     4681 ccgcaatggc accacccatg tgatcttcga gccgctggac ttcatcgcca aactcgctgc
     4741 gttggtacct aagccgcgag tcaacctcac acgcttccac ggcgtctttg caccgaacag
     4801 caaacaccga gttcaagtaa cacccgccaa gcggggcaag aagcccgaca aatcggaagg
     4861 tctcgatact aactggcgtg acaagagtcc tgcagagcgc caccgcgcca tgacctggat
     4921 gcaacgcctc aagcgagtct tcaatattga tattgaagtc tgcgaacact gcggcggtca
     4981 cgtcaaagtg attgccagca tcgaagatcc gaaggtcatt gagcagattc tcaagcatct
     5041 gaaacagaaa acagccaagg cgaatgccgc caagcagcgt gagctgccac cagaacgagc
     5101 gccgccactg actcccagcc tgttcgatcc atcacagagt cgtctctttg actgacgacc
     5161 ccaaatccaa cactgctcaa cactgccaac ttttaaacgg ggcggtgggg cagtttgtat
     5221 ctctcgagct atcaggctag agattttacc gccaaatcga accttattag agcggtttag
     5281 gctggaccgg cagttaaaat tggggcttga gcggtaaacg agtgagggaa tttcaggtaa
     5341 gatacttcgg atgaggagca aaaaggtggt ttatacttcc tataccccat aaaggaggca
     5401 tccggttgcg ttaagcggaa ttcaacggtg ttgttgtcca gtttgcgcac gctttggacg
     5461 ttatcggcaa actgcaggct gtcgaagtag gggaaactgc tgccgttcac attgtgccac
     5521 ggatggtggc gatcaaagat gcgctcgaag gtaaacacca catcgtctgc attcagtttg
     5581 cgggtggggg tgaaccaggc cgtcttctga aactgaacgt cacgacgcag atggaagcgg
     5641 taggttgcgc cgttatccag cacctcccag ctttcagcaa gctcaggcac cagccgatag
     5701 gtgtagggat ctacatcaag cagtcggtca tacaattggg ccgctaacgt atcgacgatg
     5761 agaccgctgc tcgttttttg tgggttgaac gtattgactt gcccgctgac gcaatagaca
     5821 aacccactgt cacggatatc agcgtgcgga gcttgctcag gcgcagcagc agcctgacca
     5881 ctcagaaatc cagccatcac gatcagagat gataaaacca ggcgcataat tttaatggat
     5941 tatataaaga ggctatctta ctaatactta atgacatttg ccattaccgt ttgtttttgg
     6001 gagtaacagg ctcgataagc gcgagttcaa caccgcgaca tggtcaaaat tcatacctgc
     6061 tatccacttt gaatctactc tgttaactat cttagcattt tcatggccca tctgacttgc
     6121 tataaacgtg ggggcgtacc tgtcgttgtt tactgattga tatcagtgtg tgctcggaag
     6181 atggagtatg gcatcgataa cgaagtgtgg agcgagcatt tagagtgtca ctatcaatgc
     6241 tgaactataa tcattcagga agcagtacac gttatgtgag ttgtaatagc taaagtatct
     6301 ataattattt caatatgtta attccatttt ttcttcatgg catcttaatc ttctcaccca
     6361 tttatggtga tgatttccat tggttattaa atgcattttg gctttgatcc tggttatgaa
     6421 cggttttctg ttgccattta acttctaatg gaatgaattg tatgaataaa ttactgagcc
     6481 ttgttgtttc acttgcccta ttgcctggag ccgtttttgc agcgtcgact ccagactgtg
     6541 tgaaggtgaa taaagcgcaa atcgaagcgc tatttgataa gtggaacgag tcattgaaaa
     6601 caggtaacgc gcagacggta tctgaaaatt atcttagcga tgcggtattg ttgcctactg
     6661 tatccaataa agcgcgttta acggacgctg aacgtgttga ttatttcgag cattttcttg
     6721 cgaaaaagcc gaccgggaaa attgacatgc gtaccattcg cctggggtgt aataaggcga
     6781 tagacaccgg tacctacaca tttaccttcg cggacaaatc aacggtttca gcccgctaca
     6841 cattcactta tgcatgggac ggtaaagagt ggaaaatttc cacacaccac tcttcagcga
     6901 tgcctgaagg gtaaaaagct gtgcaggggg cggaatattc accgctccct gaagactagc
     6961 caataatcgc gatgccaaga cgttccatga gcaacgatgc ctggtagttg tccaacttaa
     7021 cgccttgtaa atcaacgcgc cgaatatcta agtcacccaa ctccgaattg gtcagatcgc
     7081 aatgtgtgaa gtttgctgct cgccagtcga aagtcgaaaa ctcgccgccg gagagatctg
     7141 aaccactgaa cgtcgcgccc agtacctggg cccccatcca acggttttcc cacagctcac
     7201 acttttccaa cacgactttc gaaaaattgg cgtagcttag atttgtgtta gtgatatatg
     7261 cactgcaaaa ccaggtgcga gtagtgatca tattcataaa acttgcgccg cgaaaatctg
     7321 cgccttgcgc acgacagtgg ctaatttcaa tgccaagcgc actggcattg cgaaaatccg
     7381 ccatggataa atcacagctt ttaaaaatgg catctttcag catcgcacga ctaaaattgc
     7441 accctttctg gctttcacga tcatagaact gacagccgat aaatggcact gttgcaaagt
     7501 tagcgatgag gcagcctttt gtcttattca aaggccttac atttcaaaaa ctctgcttac
     7561 caggcgcatt tcgcccaggg gatcaccata ataaaatgct gaggcctggc ctttgcgtag
     7621 tgcacgcatc acctcaatac ctttgatggt ggcgtaagcc gtcttcatgg atttaaatcc
     7681 cagcgtggcg ccgattatcc gtttcagttt gccatgatcg cattcaatca cgttgttccg
     7741 gtacttaatc tgtcggtgtt caacgtcaga cgggcaccgg ccttcgcgtt tgagcagagc
     7801 aagcgcgcga ccataggcgg gcgctttatc cgtgttgatg aatcgcggga tctgccactt
     7861 cttcacgttg ttgaggattt tacccagaaa ccggtatgca gctttgctgt tacgacggga
     7921 ggagagataa aaatcgacag tgcggccccg gctgtcgacg gcccggtaca gatacgccca
     7981 gcggccattg accttcacgt aggtttcatc catgtgccac gggcaaagat cggaagggtt
     8041 acgccagtac cagcgcagcc gtttttccat ttcaggcgca taacgctgaa cccagcggta
     8101 aatcgtggag tgatcgacat tcactccgcg ttcagccagc atctcctgca gctcacggta
     8161 actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg
     8221 ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca
     8281 ccaccgacta tttgcaacag tgccgccgag aggatcaagc gacgcggcat gtgtcagtcg
     8341 tccgtgcgga ggtactggta gagggtttcc cggctgatgt tgaactcgcg ggcaagctgc
     8401 gccttgggct cgccggccgt cgctcgctgc cgcagggtag cagcctgctc atcggacagg
     8461 gctttcttgc ggccccggta cgcgccacgc tgcttggcca aggcgatgcc ctcacgctgc
     8521 cgctcgcgga tcagggcgcg ctcgaactca gcgaaggccc ccatcaccga cagcatcagg
     8581 ttggccatcg gcgagtcctc gccagtgaac accaggccct ccttcaggaa ctcgatgcgc
     8641 acgccgcgct gagtcagctt ctgtaccaag cgacgcaggt catcgaggtt gcgggccagc
     8701 cgatccatgc tgtgcaccac cactgtatcg ccttcgcgga cgaagctcag cagcgcttcg
     8761 agctgggggc gctgggtgtc cttgcccgat gccttgtcgg tgaacacctt gctcacctgg
     8821 gtttgttcca gctggcgttc cgggttctgg tcaaagctgc tgacccggac gtagccgatg
     8881 cggtgcccct gcacgatgtc tccttggttg aaggcggctt aagtgcactt tctgttccgt
     8941 tgtgcctcaa agcccatttc tgtcaggctg aaatctataa ccttcgcggg catgtgtcaa
     9001 aaaatgggaa agcagactct attctgacga agcggcgcgg ccctgcctga catcaagtta
     9061 gggtatagcc tagattgaca tgcgcgatgc aacccttaac ttgcttgcac ctatcgtttc
     9121 catgctagct ttatcgtaac gctaaggaag ctctgaaaaa gcccatgttt cgcgaaatca
     9181 ccgctctgta acccgcatgg ttgctgggat gcatttctcg agcgggggct ttttcagacc
     9241 ttccttagct tagcgtacga ttttttccga attctgcggt tccccctgga agacctacgc
     9301 aagttgggcc agctcagagg tggaatcaac gaaggcgagc gaatgaggca tcttttctgg
     9361 cgtcttaact ttctccttct ctccggggca acaaacagcg cccagatgta ccatgaggcc
     9421 gccgctcttt cctccgagct ggatactcgt tggtcttacc gctccaaaga gcttatgacg
     9481 ctctacagca aggcgaaatc ctatgaggct ggggaacgtg tcgagttcgg cggaaagtcg
     9541 tttgcaccgc tatacacgcc caaaaacgac acgctgatca acctattcca gatctcacag
     9601 caagaacaag agcaactgaa gacgattatc agcactgacg aggctcagaa gcgccgcaga
     9661 gagcgcgata gactgcgtga tgaagaacgt cgccgcgccg ctggccagct tgagcgtgaa
     9721 gcctatgaag ccaactcatt gagcaggcaa aagccctggg aagctatggg catgagccgg
     9781 gctaaatggt atcgcctggg caagccttca cctcaacaaa acagtgagac aagtccctcc
     9841 cctattacta atggcgaagc ctcagcggtt gcccttcagg cggccaggtg taaagccgtt
     9901 tagatggcta tttcaaagtg agtgagattt cgtaacggat ggcagcccta gaggctgctt
     9961 tcttttgagg ggttagcagg cgtatagcca tgctggatct cagtctatca ctacctgctt
    10021 aatattccac ctttagatcc ggcagcagtt cgaccaggtg gccaacttac caggcaccgg
    10081 cagcagctcg accgggtggc caagttcaga tctggacgcc agaaggaaat caaccaggtg
    10141 gccaacttac caggcaccgg cagcagctcg accaggtggc caagttcaga tctggacgcc
    10201 agaaggaaat caaccaggtg gccaactcac caggcatcgg cagcagctcg accaggtggc
    10261 ccatttcgta cgtacgaaat caggcgacgc tatgcgaaat cttggcggca atcccccgcg
    10321 actacgtgat ttttttaacg tagagcgcgc tttttcttgc catagtaggc cattttggcc
    10381 tataatctta attaagaaga ccgggcaccc gccacggtca ataccggaga actccgatga
    10441 tccacacagc taaccgcacc tttcaccaac tctatcgaga atggatacgc gaacgccgcg
    10501 agcatatgca caacgttttg acctgggagc gtgatcgtta cggtgctcgc ctcgtcggtc
    10561 tgttttatcg ttactgcaag gtggccaacc cctttccccg ttgcactctg aatactcgga
    10621 tcaactaccg cgcccatgcg gtgaatctgc cagattggcc agcccgcagc ctggagctga
    10681 ataaaatgtg gctcagttgg agagaaaaaa agtaggccga tttggcctat tttttattgt
    10741 acccgcttga cacaataggc caaaatggcc tattatatat ctcagaaggc cgggcacccg
    10801 ccacggtcaa taccggagaa ctccgatgat gcaaacagaa cttaatcccc tgatctgctc
    10861 cctggtcgcc acccctcgcc gtatggctgc aatgccccgc tatgtaggcc gtttctatgt
    10921 ggttttcgag tcgatgcttt accagcaaat gaaagggctc tgccgcgagt atcgcggggc
    10981 ttattggctt atgtgggagc tgtcgaacgg cggcttttat atggctcctg ggcgtcgtga
    11041 tgaaatgctg aacatcgagg ccatgaacta ctttagtggc caaatgagcg ccgatgctgc
    11101 cgggattacc gcttgcctct acctctatag ccatctgtca tttcacacag aaggcgctga
    11161 ccaggagcgg ttttcaaggc tgtatcacag cttgcgggat tgggcttgtg agcatgacga
    11221 aaaagaggcc atcttggccg caatagacta gcaacagaaa gccccggtga tggccggggc
    11281 tcttaaagga ggtgcatagg tagaaaaaga aatggtgccc ctggtatggc tgcaaccaat
    11341 cacaggggct ttcaccggtc aatttgagga taaacgcgat gaatacggat attgtatcac
    11401 ttgcaaaaca cattggcgac gctctgacgg cccgccgccc cgttcatatg ccgcgcatga
    11461 agatgcggga gcttggcttg ctcttgattg aactgaaggc gtatcgggag gccgccgcga
    11521 ccgttaccca ttgataaaaa agccccggca ttgaccgggg ctatttgagg atttatcgca
    11581 tgattagacc tgaaacgctt aggccgttcg ctgaagattg gcaggctcca accgccgatg
    11641 aaatcaaaga ggtgttggag ctgattaggc aacgaaaagg gcttagtaag cctctcagtg
    11701 gcgttgatgt cgctgatctc gttggtttgc ctggtgagag aggcagcgga aagggaaccc
    11761 gaaccttcag gcgttgggtg agcaaaacca accctagccc tattgcttat ggtgcctggt
    11821 ctatcttggc ccacctggca ggtttcgggg ctatatggga cgctgaccgg gattgaaacc
    11881 atgaccgaac ataaggccga acgtgctccc tggggagact tcccggcagt agtgaggaac
    11941 ggcgatctaa aggatctctc taaagaacct gaatacgaag ccgccaaaca tggcgaccac
    12001 aaggcgatga gctataagcg catgaagccg gccgaggacg aattgcattg cgagatcaag
    12061 gcgctgcttg atcgcgccaa ggctaccgac gaccaggagc gtaacgagcc ggagctggac
    12121 attcctgccg agatttctcg ccgcgagaag cgcctggagg cgatccaggc ggcaaaggcg
    12181 cgcctggaag cgcgccagcg tgaagcggac caggcccggg ggcgcagcga agacgatggc
    12241 cgccggcctc gccatccgga tggctcggac aagggcggtg gctcgtacaa acgcgagttt
    12301 ggtgtgccgg atgaccgtga tcaggaaagc ttcaccgatc cggacagccg gatcatgaaa
    12361 cacgccggtg gtggctccga gcagagctac aacgggtaca cagcggtcga tgccgagcac
    12421 cagatcatcg tggcggcgga gttgaccaac tgcgccgcgg acagtcaggc gctgctgggc
    12481 atgctggcag cagttcaggc caacaccgga gaaatgccgg cccagacgct ggcggatgcg
    12541 ggattccgta gtgaggctgt tctggcaaag gtcgccgatc accacggcga tgtcatcgtt
    12601 gccctcggcc gcgagggacg tgaagatgcc aaggtcaatg ccaagaccca tccgcatacg
    12661 gcggcgattg cggcgaaatt gaaaacggag caaggcgatg cagcttaccg ccggcgcaag
    12721 tcgatcgtgg aggctccgaa tggttggatc aaggcggtga tgggattgcg tcagttcagc
    12781 atgaggggcc tggacaaggt gcaagccgag tggaagctcg tctgcatggc gctaaatctg
    12841 aggcgaatgg cgtatctgtg agggcgaagg ttaaatgggg cggctcaaat gcaccccagt
    12901 cgtcataaca cgccgcgcgc cgcaagattg gtatccttgg cgccgacgcc ttgccaattg
    12961 cagagagcgc cgccgccatc gtcagtgctc tacagaaaac cggtcacacg gctctgccgc
    13021 gcagactcct agcctaaatg tgacagtggt tggtaatcca tgccgcccgc aagggcggcg
    13081 gtggtgggcc aatagatgat tttcagagcc ttactgcccg ttgacgccca atccctcgag
    13141 cgcgagtcta gccgcagcgg cgatgacggc ctcgctgtgc ttgtcatcct tgttaggcgc
    13201 ccgggtgtag acggccaaca caataggtgc gcgcccagtg ggccagacga cggcatagtc
    13261 atttgccgtg ccatacactc cgcaggttcc ggttttgtct ccgactgccc agtctgccgg
    13321 caccgccgcg cggatgcggt ggttgccggt cgtgtttccc tttagccaat caacaaactg
    13381 ctgccgctgc ggcgcagcca gtgcagagcc cagtgtcagt ttttgtaagc tttccgtcac
    13441 ggcgcgcggc gatgaggtat cgcgcgcatc gcctgggatg gcggagttca gctccagctc
    13501 ccagcggtcc agacggaacg tggtatcgcc gatagagcgc atgaaggccg tcagcccggc
    13561 cgggccgccc aactccttca gcaacaaatt ggcggcggcg ttatcactgt attgcacggc
    13621 ggccgcggac agctccgcca ccgtcatgcc tgttgtcaga tatttttccg agatgggtga
    13681 ccacggaacc agcgcatttt tgccgtaacg gatgggtgtg tccagcaagc cggcctgctg
    13741 ctggctgcga gccagcacag cggcagcaag aaagcccttg aatgagctgc acagtgggaa
    13801 gcgctcctca gcgcggtaac ttacagttgc gcctgagccg gtatccatcg cgtacacacc
    13861 gatggagccg ccaaagtcct gttcgagttt agcgaatggt tccgcgacga ggttggtcag
    13921 cgcggtggca gaaaagccag ccagcggcca tgagagacaa gacagcagaa ctagacggcg
    13981 atacagtgac atcaacgata ttccttgttt gaaggtggag ctaggtgtag cggccggatg
    14041 taatcaggcc gctacagctg ggattagggg tctgacgctc agtggaacga aaactcacgt
    14101 taagaagtca tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    14161 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    14221 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta tatgtcaaga
    14281 cccggctggt tatacacgcg tttcctgaac aattcagggc gtttttcatg ccattccttg
    14341 agcgcctgaa cgggtgtttt gtgttggagc gcgcgttgcg gaatgctgtg gttgtagatc
    14401 ttgacgtaat tgcgcagcgt cgattccagt tcggcagctg aaccaaaacg ggtctggttg
    14461 acgatgtcgc tgatacgacc gttgaagcgc tccaccatgc cgttggtctg cggatgacga
    14521 ggcgggatga gccggtgctc gatgccgagc tgcttgcaca ggcggtcgaa cacgtgtgtg
    14581 ccgctgggtt ccttcttctt gccgccagcc gtgaagcggt cggtgaactg gctgccgttg
    14641 tcggtcagaa gcttgacgat cttgacggga caggcttgct ggactttgtt gaggaagtcg
    14701 ccactgctgc catcggtctg gtcggcatag agctcgatga agacccagcg cgtagcacgg
    14761 tcgatggcaa ggcactgttg caaagttagc gatgaggcag ccttttgtct tattcaaagg
    14821 ccttacattt caaaaactct gcttaccagg cgcatttcgc ccaggggatc accataataa
    14881 aatgctgagg cctggccttt gcgtagtgca cgcatcacct caataccttt gatggtggcg
    14941 taagccgtct tcatggattt aaatcccagc gtggcgccga ttatccgttt cagtttgcca
    15001 tgatcgcatt caatcacgtt gttccggtac ttaatctgtc ggtgttcaac gtcagacggg
    15061 caccggcctt cgcgtttgag cagagcaagc gcgcgaccat aggcgggcgc tttatccgtg
    15121 ttgatgaatc gcgggatctg ccacttcttc acgttgttga ggattttacc cagaaaccgg
    15181 tatgcagctt tgctgttacg acgggaggag agataaaaat cgacagtgcg gccccggctg
    15241 tcgacggccc ggtacagata cgcccagcgg ccattgacct tcacgtaggt ttcatccatg
    15301 tgccacgggc aaagatcgga agggttacgc cagtaccagc gcagccgttt ttccatttca
    15361 ggcgcataac gctgaaccca gcggtaaatc gtggagtgat cgacattcac tccgcgttca
    15421 gccagcatct cctgcagctc acggtaactg atgccgtatt tgcagtacca gcgtacggcc
    15481 cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc
    15541 agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc catcggcgta
    15601 gtagtggatg tggtcgatga caaagccggt gcgggtcagc gtgcgccgga ggatcggcag
    15661 aaaatcgacc aggaacgaag tagcgcgtgt gacgacggcc ggtacgccga cacgcgccac
    15721 ggcctcggcc cagcgcgcgg ccggcggttg gagcaggccg ttgtgcaccg aaccgtggta
    15781 ggtgccgacc gccaatgtga gccagcgctc tagctcgcgc agcgtcaggg cggccttgtt
    15841 ttcggaatcg tagtcgccgc gctggtcagg gttggagaag gtcgttcccg gcagttcgtc
    15901 gtgaatcatc tgcatcgccg tgccgatgat ccgttccacg atgccgccat agtgcggctg
    15961 tcccagcggg cgatagtcca gccggatgcc atgctgctcg caaccccggc gcagggcctc
    16021 gctcttgaac tcggccgcgt tgtctaggta gagcagcaag ggcttgccgc tcatctgcca
    16081 atccatttcc acgttcagtc cttccagcca agggcgcttg tcgcaggcga catgcacgag
    16141 gcacaggcca accgaaacgg cagacggcgc ttccagcgtg acgaccatgc cgagcacgca
    16201 gcgggtgaac acgtcgatgg cgagggtcag gtacgggcgg ccaataggtt gccggtcgcg
    16261 gtcatcgacc acgatcaggt cgatgaccgt atggtctatc tgcacctgct ccagcggcgc
    16321 ggtcacggca ggaggctcgc cgcccacacc ttgtaggtca cgagcggcat cctggccttc
    16381 ccgccggcgg atgaccttgc gcgggtcaag gctagcgatc cgtaaggcca cggtattgcg
    16441 cgccggcact cgcagttttt gagccttgca cacctgagtg acttcgcggt gaaaggccgc
    16501 taggctgcgc ttctgcttgg tcaggaaccg cttttgcagt agctcgtgga tgacgcgctc
    16561 gaccggttcc ggcaagcgcc ccttaccttt acctccaccg gactggccgg gcaccagatc
    16621 cgtcacgagg ccgctgcctt gccgggcacg ccggatcaga acgtatacct ggcgccgaga
    16681 caagcccagc gcctgagccg ccatatcggc cgcttcgtgc ccgaccgtct ccgactgcgc
    16741 caacggactg atgatctccg cacgacggcg cgcacgctcc caagcctcat caggcagagt
    16801 ggccacgcct tgttctggaa tccgtggggt gtccgtcgcc atgctcacct cgctttggtg
    16861 cacacgagta ttgagcatag tcgagattgg tgcagatcac ttctgatatt gaactgtcag
    16921 gagctggctg cacaacagcc attacgccca atcaactggt gcagtcgtct tctgaaaatg
    16981 aca
//
