LOCUS       In2-8                   4960 bp    DNA     linear   BCT 27-OCT-2020
DEFINITION  Acinetobacter baumannii.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria;
            Pseudomonadales; Moraxellaceae; Acinetobacter; Acinetobacter
            calcoaceticus/baumannii complex; Acinetobacter baumannii.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of In2-8
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
     mobile_element  1..4960
                     /gene="In2-8"
                     /product="Class 2 integron: In2-8"
     CDS             complement(1..537)
                     /gene="intI2"
                     /codon_start=1
                     /transl_table=11
                     /product="Integrase"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     regulatory      644..671
                     /gene="Pc2A"
                     /product="Promoter Pc2A"
     regulatory      644..649
                     /gene="-35 region_Pc2A"
                     /product="-35 region of Pc2A"
     regulatory      666..671
                     /gene="-10 region_Pc2A"
                     /product="-10 region of Pc2A"
     regulatory      752..779
                     /gene="Pc2B"
                     /product="Promoter Pc2B"
     regulatory      752..757
                     /gene="-35 region_Pc2B"
                     /product="-35 region of Pc2B"
     regulatory      774..779
                     /gene="-10 region_Pc2B"
                     /product="-10 region of Pc2B"
     misc_recomb     793..855
                     /gene="attI2"
                     /product="attI2 site"
     CDS             860..1384
                     /gene="sat2"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptothricin acetyltransferase"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     misc_recomb     1379..1437
                     /gene="attC_sat2"
                     /product="attC site for sat2"
     CDS             1442..1975
                     /gene="aadB4"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside adenyltransferase"
                     /translation="MDTTQVTLIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDD
                     IDLTFPGERRGELEAMVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEA
                     PQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLG
                     AEKVEVLRAAFRSRYAA"
     misc_recomb     1970..2029
                     /gene="attC_aadB4"
                     /product="attC site for aadB4"
     CDS             2070..2702
                     /gene="catB2"
                     /codon_start=1
                     /transl_table=11
                     /product="Chloramphenicol acetyltransferase"
                     /translation="MTNYFESPFKGKLLTEQVKNPNIKVGRYSYYSGYYHGHSFDDCA
                     RYLLPDRDDVDQLIIGSFCSIGSGAAFIMAGNQGHRYDWVSSFPFFYMNEEPAFAKSV
                     DAFQRAGDTVIGSDVWIGSEAMIMPGIKIGHGAVIGSRALVAKDVEPYTIVGGNPAKS
                     IRKRFSEEEISMLLDMAWWDWPLEQIKEAMPFLCSSGIASLYRRWQGTSA"
     misc_recomb     2697..2702
                     /gene="truncated attC_catB2"
                     /product="Truncated attC site for catB2"
     misc_recomb     2703..2940
                     /gene="attI2"
                     /product="attI2 site"
     CDS             2961..3434
                     /gene="dfrA1"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydrofolate reductase type I"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     misc_recomb     3429..3523
                     /gene="attC_dfrA1"
                     /product="attC site for dfrA1"
     CDS             3529..4053
                     /gene="sat2"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptothricin acetyltransferase"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     misc_recomb     4048..4107
                     /gene="attC_sat2"
                     /product="attC site for sat2"
     CDS             4111..4899
                     /gene="aadA1y"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptomycin 3''-adenyltransferase"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_recomb     4901..4960
                     /gene="attC_aadA1y"
                     /product="attC site for aadA1y"
BASE COUNT     1324 a   1107 c   1267 g   1262 t
ORIGIN
        1 ttattttatt gctgggatta ggcgcgtggg cagtaggctg tttctgcttt tcccaccctt
       61 accgtcatgc acagtgatgc agccattatc aaaatcaaaa tctttaaccc gcaaacgcaa
      121 gcattcatta atgcgcaaac ctgcaccata cagcagcgta aaaataactt ggttgcgagt
      181 atccataacc tgcaaaatgc gttgcacttc atttgcagag ataacagagg gtagccgtct
      241 aggcttgctt gcagggatat aatcaatatc gcccaacggc tgttgtaaaa acctgttgta
      301 caaaaaagct agggcattta aagcgatttt ctgcgtgttt atggctacat gtctgctgtt
      361 tgctaagctg gataaaaaca gcctgacctc ttcactgccc atggtctgag gatgacgttt
      421 tttgtgaaac agaataaaac gcttaatcca gtgcaggtaa gttttttcag ttttcagcgc
      481 ataacctttt tgtcgcatat ccgtgcgtat agaatttaaa aatggactgt tagacataaa
      541 acgctccttg tcttgcaact gtctgcctat acagcctatt ctagctggga tttaaaaaag
      601 tgcctgtttt ttacgcctag agatgcttgt ttaccggtag agttttaatt taatgctaaa
      661 taaattaaaa tgttatgagt tctttgggtg agataatgtg catcgtgcaa gcaggataga
      721 cggcatgcac gatttgtaat aacagagtgt cttgtatttt taaagaaagt ctatttaata
      781 caagtgatta tattaattaa cggtaagcat cagcgggtga caaaacgagc atgcttacta
      841 ataaaatgtt aggcgtcata tgaagatttc ggtgatccct gagcaggtgg cggaaacatt
      901 ggatgctgag aaccatttca ttgttcgtga agtgttcgat gtgcacctat ccgaccaagg
      961 ctttgaacta tctaccagaa gtgtgagccc ctaccggaag gattacatct cggatgatga
     1021 ctctgatgaa gactctgctt gctatggcgc attcatcgac caagagcttg tcgggaagat
     1081 tgaactcaac tcaacatgga acgatctagc ctctatcgaa cacattgttg tgtcgcacac
     1141 gcaccgaggc aaaggagtcg cgcacagtct catcgaattt gcgaaaaagt gggcactaag
     1201 cagacagctc cttggcatac gattagagac acaaacgaac aatgtacctg cctgcaattt
     1261 gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg ttcacgtata aaactagacc
     1321 tcaagtctcg aacgaaacag cgatgtactg gtactggttc tcgggagcac aggatgacgc
     1381 ctaacaattc attcaagccg acaccgcttc gcggcgcggc ttaattcagg agttaggccg
     1441 catggacaca acgcaggtca cattgataca ccaaattcta gctgcggcag atgagcgaaa
     1501 tctgccgctc tggatcggtg ggggctgggc gatcgatgca cggctagggc gtgtaacacg
     1561 caagcacgat gatattgatc tgacgtttcc cggcgagagg cgcggcgagc tcgaggcaat
     1621 ggttgaaatg ctcggcgggc gcgtcacgga ggagttggac tatggattct tagcggagat
     1681 cggggatgag ttacttgact gcgaacctgc ttggtgggca gacgaagcgt atgaaatcgc
     1741 ggaggctccg cagggctcgt gcccagaggc ggctgagggc gtcatcgccg ggcggccagt
     1801 ccgttgtaac agctgggagg cgatcatctg ggattacttt tactatgccg atgaagtacc
     1861 accagtggac tggcctacaa agcacataga gtcctacagg ctcgcatgca cctcactcgg
     1921 ggcggaaaag gttgaggtct tgcgtgccgc tttcaggtcg cgatatgcgg cctaacaatt
     1981 cgtccaagcc gacgccgctt cgcggcgcgg cttaactcag gtgttaggcg acgcgtggag
     2041 tcgctctaga attttcgggt acaaatttta tgacgaatta ttttgagagt cccttcaaag
     2101 ggaagcttct gactgagcag gtgaagaatc cgaacatcaa ggtagggcgg tatagctact
     2161 attccggcta ttaccatggg cactcgtttg atgattgtgc tcgctacctt ctaccagacc
     2221 gtgatgacgt tgatcagctg attatcggca gcttctgctc catcggatca ggcgcagctt
     2281 ttattatggc tgggaatcaa ggccaccgat atgattgggt ctcttctttc cctttcttct
     2341 acatgaacga ggagcccgcg tttgcaaaat cagtcgatgc attccagcgg gctggcgaca
     2401 cagttatagg aagtgatgtg tggatcggtt cggaggccat gatcatgccc gggatcaaga
     2461 tcgggcatgg agcggtgata ggtagccgcg ctttggttgc caaagacgtg gaaccctaca
     2521 ccatagtggg gggaaaccct gcaaagtcga ttaggaagcg cttttctgaa gaagaaattt
     2581 ctatgctttt agatatggct tggtgggatt ggccgctgga acaaatcaag gaagcaatgc
     2641 cttttctttg ttcgtctggc attgccagcc tgtatcgtcg ctggcaaggc acaagcgcct
     2701 aattacgcct agagatgctt gtttaccggt agagttttaa tttaatgcta aataaattaa
     2761 aatgttatga gttctttggg tgagataatg tgcatcgtgc aagcaggata gacggcatgc
     2821 acgatttgta ataacagagt gtcttgtatt tttaaagaaa gtctatttaa tacaagtgat
     2881 tatattaatt aacggtaagc atcagcgggt gacaaaacga gcatgcttac taataaaatg
     2941 ttaacctctg aggaagaatt gtgaaactat cactaatggt agctatatcg aagaatggag
     3001 ttatcgggaa tggccctgat attccatgga gtgccaaagg tgaacagctc ctgtttaaag
     3061 ctattaccta taaccaatgg ctgttggttg gacgcaagac ttttgaatca atgggagcat
     3121 tacccaaccg aaagtatgcg gtcgtaacac gttcaagttt tacatctgac aatgagaacg
     3181 tattgatctt tccatcaatt aaagatgctt taaccaacct aaagaaaata acggatcatg
     3241 tcattgtttc aggtggtggg gagatataca aaagcctgat cgatcaagta gatacactac
     3301 atatatctac aatagacatc gagccggaag gtgatgttta ctttcctgaa atccccagca
     3361 attttaggcc agtttttacc caagacttcg cctctaacat aaattatagt taccaaatct
     3421 ggcaaaaggg ttaacaagtg gcagcaacgg attcgcaaac ctgtcacgcc ttttgtacca
     3481 aaagccgcgc caggtttgcg atccgctgtg ccaggcgtta ggcgtcatat gaagatttcg
     3541 gtgatccctg agcaggtggc ggaaacattg gatgctgaga accatttcat tgttcgtgaa
     3601 gtgttcgatg tgcacctatc cgaccaaggc tttgaactat ctaccagaag tgtgagcccc
     3661 taccggaagg attacatctc ggatgatgac tctgatgaag actctgcttg ctatggcgca
     3721 ttcatcgacc aagagcttgt cgggaagatt gaactcaact caacatggaa cgatctagcc
     3781 tctatcgaac acattgttgt gtcgcacacg caccgaggca aaggagtcgc gcacagtctc
     3841 atcgaatttg cgaaaaagtg ggcactaagc agacagctcc ttggcatacg attagagaca
     3901 caaacgaaca atgtacctgc ctgcaatttg tacgcaaaat gtggctttac tctcggcggc
     3961 attgacctgt tcacgtataa aactagacct caagtctcga acgaaacagc gatgtactgg
     4021 tactggttct cgggagcaca ggatgacgcc taacaattca ttcaagccga caccgcttcg
     4081 cggcgcggct taattcagga gttaaacatc atgagggaag cggtgatcgc cgaagtatcg
     4141 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     4201 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     4261 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     4321 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     4381 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     4441 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     4501 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     4561 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     4621 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     4681 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     4741 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct
     4801 tatcttggac aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac
     4861 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc
     4921 cgacgccgct tcgcggcgcg gcttaactca agcgttagag
//
