LOCUS       In2-4                   2868 bp    DNA     linear   BCT 27-OCT-2020
DEFINITION  Escherichia coli.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia; Escherichia
            coli.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of In2-4
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
     mobile_element  1..2868
                     /gene="In2-4"
                     /product="Class 2 integron: In2-4"
     CDS             complement(1..537)
                     /gene="intI2"
                     /codon_start=1
                     /transl_table=11
                     /product="Integrase"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     regulatory      644..671
                     /gene="Pc2A"
                     /product="Promoter Pc2A"
     regulatory      644..649
                     /gene="-35 region_Pc2A"
                     /product="-35 region of Pc2A"
     regulatory      666..671
                     /gene="-10 region_Pc2A"
                     /product="-10 region of Pc2A"
     regulatory      752..779
                     /gene="Pc2B"
                     /product="Promoter Pc2B"
     regulatory      752..757
                     /gene="-35 region_Pc2B"
                     /product="-35 region of Pc2B"
     regulatory      774..779
                     /gene="-10 region_Pc2B"
                     /product="-10 region of Pc2B"
     misc_recomb     793..855
                     /gene="attI2"
                     /product="attI2 site"
     CDS             869..1342
                     /gene="dfrA1"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydrofolate reductase type I (trimethoprim
                     resistance protein)"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     misc_recomb     1337..1431
                     /gene="attC_dfrA1"
                     /product="attC site for dfrA1"
     CDS             1437..1961
                     /gene="sat2"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptothricin acetyltransferase"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     misc_recomb     1956..2015
                     /gene="attC_sat2"
                     /product="attC site for sat2"
     CDS             2019..2807
                     /gene="aadA1y"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptomycin 3''-adenyltransferase"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_recomb     2809..2868
                     /gene="attC_aadA1y"
                     /product="attC site for aadA1y"
BASE COUNT      799 a    637 c    685 g    747 t
ORIGIN
        1 ttattttatt gctgggatta ggcgcgtggg cagtaggctg tttctgcttt tcccaccctt
       61 accgtcatgc acagtgatgc agccattatc aaaatcaaaa tctttaaccc gcaaacgcaa
      121 gcattcatta atgcgcaaac ctgcaccata cagcagcgta aaaataactt ggttgcgagt
      181 atccataacc tgcaaaatgc gttgcacttc atttgcagag ataacagagg gtagccgtct
      241 aggcttgctt gcagggatat aatcaatatc gcccaacggc tgttgtaaaa acctgttgta
      301 caaaaaagct agggcattta aagcgatttt ctgcgtgttt atggctacat gtctgctgtt
      361 tgctaagctg gataaaaaca gcctgacctc ttcactgccc atggtctgag gatgacgttt
      421 tttgtgaaac agaataaaac gcttaatcca gtgcaggtaa gttttttcag ttttcagcgc
      481 ataacctttt tgtcgcatat ccgtgcgtat agaatttaaa aatggactgt tagacataaa
      541 acgctccttg tcttgcaact gtctgcctat acagcctatt ctagctggga tttaaaaaag
      601 tgcctgtttt ttacgcctag agatgcttgt ttaccggtag agttttaatt taatgctaaa
      661 taaattaaaa tgttatgagt tctttgggtg agataatgtg catcgtgcaa gcaggataga
      721 cggcatgcac gatttgtaat aacagagtgt cttgtatttt taaagaaagt ctatttaata
      781 caagtgatta tattaattaa cggtaagcat cagcgggtga caaaacgagc atgcttacta
      841 ataaaatgtt aacctctgag gaagaattgt gaaactatca ctaatggtag ctatatcgaa
      901 gaatggagtt atcgggaatg gccctgatat tccatggagt gccaaaggtg aacagctcct
      961 gtttaaagct attacctata accaatggct gttggttgga cgcaagactt ttgaatcaat
     1021 gggagcatta cccaaccgaa agtatgcggt cgtaacacgt tcaagtttta catctgacaa
     1081 tgagaacgta ttgatctttc catcaattaa agatgcttta accaacctaa agaaaataac
     1141 ggatcatgtc attgtttcag gtggtgggga gatatacaaa agcctgatcg atcaagtaga
     1201 tacactacat atatctacaa tagacatcga gccggaaggt gatgtttact ttcctgaaat
     1261 ccccagcaat tttaggccag tttttaccca agacttcgcc tctaacataa attatagtta
     1321 ccaaatctgg caaaagggtt aacaagtggc agcaacggat tcgcaaacct gtcacgcctt
     1381 ttgtaccaaa agccgcgcca ggtttgcgat ccgctgtgcc aggcgttagg cgtcatatga
     1441 agatttcggt gatccctgag caggtggcgg aaacattgga tgctgagaac catttcattg
     1501 ttcgtgaagt gttcgatgtg cacctatccg accaaggctt tgaactatct accagaagtg
     1561 tgagccccta ccggaaggat tacatctcgg atgatgactc tgatgaagac tctgcttgct
     1621 atggcgcatt catcgaccaa gagcttgtcg ggaagattga actcaactca acatggaacg
     1681 atctagcctc tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa ggagtcgcgc
     1741 acagtctcat cgaatttgcg aaaaagtggg cactaagcag acagctcctt ggcatacgat
     1801 tagagacaca aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt ggctttactc
     1861 tcggcggcat tgacctgttc acgtataaaa ctagacctca agtctcgaac gaaacagcga
     1921 tgtactggta ctggttctcg ggagcacagg atgacgccta acaattcatt caagccgaca
     1981 ccgcttcgcg gcgcggctta attcaggagt taaacatcat gagggaagcg gtgatcgccg
     2041 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     2101 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     2161 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     2221 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     2281 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     2341 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     2401 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     2461 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     2521 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     2581 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     2641 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     2701 ggcaggctta tcttggacaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     2761 ttgttcacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc
     2821 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagag
//
