LOCUS       In2-15                  5974 bp    DNA     linear       27-OCT-2020
DEFINITION  Providencia stuartii.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Providencia stuartii
  ORGANISM  Providencia stuartii
            Unclassified.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of In2-15
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Providencia stuartii"
                     /mol_type="genomic DNA"
     mobile_element  1..5974
                     /gene="In2-15"
                     /product="Class 2 integron: In2-15"
     CDS             complement(1..978)
                     /gene="intI2"
                     /codon_start=1
                     /transl_table=11
                     /product="Integrase"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFALLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLISAIKQLIEQARLIQQDDNLQGVGPSLPFALDRKYPSAYRQAAWM
                     FVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSKRVTCHTFRHSFATHL
                     LQAGRDIRTVQELLGHTDVKTTQIYTHVLGQHFAGTTSPADGLMLLINQ"
     regulatory      1085..1112
                     /gene="Pc2A"
                     /product="Promoter Pc2A"
     regulatory      1085..1090
                     /gene="-35 region_Pc2A"
                     /product="-35 region of Pc2A"
     regulatory      1107..1112
                     /gene="-10 region_Pc2A"
                     /product="-10 region of Pc2A"
     regulatory      1193..1220
                     /gene="Pc2B"
                     /product="Promoter Pc2B"
     regulatory      1193..1198
                     /gene="-35 region_Pc2B"
                     /product="-35 region of Pc2B"
     regulatory      1215..1220
                     /gene="-10 region_Pc2B"
                     /product="-10 region of Pc2B"
     regulatory      1229..1256
                     /gene="Pc2B"
                     /product="Promoter Pc2B"
     regulatory      1229..1234
                     /gene="-35 region_Pc2B"
                     /product="-35 region of Pc2B"
     regulatory      1251..1256
                     /gene="-10 region_Pc2B"
                     /product="-10 region of Pc2B"
     misc_recomb     1270..1332
                     /gene="attI2"
                     /product="attI2 site"
     CDS             1348..1851
                     /gene="gcu22"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MSYRNKTYVIFDGDEDMWAYRYMRGWKANENIDFNFFDAHDLKP
                     LTDRAGEDTVKRRLRERLGDTKQAIVLIGEKTKNLYRFVRWELETCMNLDIPIIAVNL
                     NGQRSQDENLCPPIIRDEYVVHIPFKLKIIQYALDNFPGEFHRRNLSDKGPRLYNDSV
                     YKQLGIE"
     misc_recomb     2365..2796
                     /gene="attC_gcu22"
                     /product="attC site for gcu22"
     CDS             2826..3254
                     /gene="gcu23"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MSTRQIHVFISHAWKYSGHYQTLADWIFNQNWSVGQASLDFRNY
                     SVPKDDPIHDAPNDRQLRDAIMRQISMSHVVVIPTGMYTNYSKWIAKEIEGSTGFNKP
                     ILAVNPWGQQRTSSVVANAAAKIVGWNKQSVVDGIWELYK"
     misc_recomb     4140..4211
                     /gene="attC_gcu23"
                     /product="attC site for gcu23"
     CDS             4245..5393
                     /gene="gcu24"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MDEIELDFGAITIDNATYKGEGYRFYEGLLAQMRQFKESPVKVL
                     QTDIVHNEAINHIGQEISKTRSSIEQALRSANKQLKIKSEVIENARALLSVDGSEAEI
                     AEARLEKYYEFIGAEKIDSGRYADLSRLMEMYFATEAPFESGKDKKNEFPDAIALLAL
                     EGWAEENEINIIAVSQDKGWKNFSEGSDRITLVSSLAEALEKFQPHYKVVSIISHIRE
                     DSLLDGENHVLEEIEQAIINSVDGCDIWVEASSYMHFEWEDVSASYISHELDKDQDGL
                     VKVRVVSINDEEIVLKVGATVEVEVEASFDFSVRDSIDKDYVGMGGNVCTTTESYHTD
                     ILLSLTGDFSQDFDDIDVAEIEVLETIGRADFGEVEPDWRSEYEDEEL"
     misc_recomb     5388..5459
                     /gene="attC_gcu24"
                     /product="attC site for gcu24"
     CDS             5476..5898
                     /gene="gcu25"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MTLLKIIAVATMLLVLAGCSTTKTMNGIMSSWEGSNINEVVAQW
                     GYPHEEREFQGRKLYIWHHNKSAYIPQTTNTTGSVYGNSVYATSTTSGGYAIQGSCQR
                     VLEVDSSGTVVRWEWRGNNCPFGELMEYANWRKKEPAQ"
     misc_recomb     5900..5974
                     /gene="attC_gcu25"
                     /product="attC site for gcu25"
BASE COUNT     1745 a   1158 c   1473 g   1598 t
ORIGIN
        1 ttactgattg ataagtagca tcagtccatc cgcaggactg gtggtgccgg caaaatgctg
       61 acccaacaca tgcgtataga tttgcgtggt cttaacatcg gtatgcccta agagttcttg
      121 cacagtgcga atatcacgcc ccgcttgtaa tagatgcgta gcaaacgagt gacgaaatgt
      181 atgacaagtg acacgcttgc taacgatgcc tgctttttgt acggctgcct tcaatgcctt
      241 tcgcgcaacg gagtcatgca gatgatggcg gcataatttg ccgttatacg ggtggttgca
      301 gagcgtgctg gagggaaaga caaacatcca cgccgcttgt cgataagcag aagggtattt
      361 gcgatctaaa gcaaaaggca gcgatggccc tacgccttgt aagttgtcgt cttgctgaat
      421 aagccgcgct tgctcaatga gttgttttat tgctgagatt aggcgcgtgg gcagtaggct
      481 gtttctgctt ttcccaccct taccgtcatg cacagtgatg cagccattat caaaatcaaa
      541 atctttaacc cgcaaacgca agcattcatt aatgcgcaaa cctgcaccat acagcagcgc
      601 aaaaataact tggttgcgag tatccataac ctgcaaaatg cgttgcactt catttgcaga
      661 gataacagag ggtagccgtc taggcttgct tgcagggata taatcaatat cgcccaacgg
      721 ctgttgtaaa aacctgttgt acaaaaaagc tagggcattt aaagcgattt tctgcgtgtt
      781 tatggctaca tgtctgctgt ttgctaagct ggataaaaac agcctgacct cttcactgcc
      841 catggtctga ggatgacgtt ttttgtgaaa cagaataaaa cgcttaatcc agtgcaggta
      901 agttttttca gttttcagcg cataaccttt ttgccgcata tccgtgcgta tagaatttaa
      961 aaatggactg ttagacataa aacgctcctt gtcttgcaac tgtctgccta tacagcctat
     1021 tctagctggg atttaaaaaa gtgcctgttt tttacgccta gagatgcttg tttaccggta
     1081 gagttttaat ttaatgctaa ataaattaaa gtgttatgag ttctttgggt gagataatgt
     1141 gcatcgtgca agcaggatag acggcatgca cgatttgcaa taacatagtg tcttatattt
     1201 ttaaagaaag tctatttaat acaagtgatt atatttttaa agaaagtcta tttaatacaa
     1261 gtgattatat taattaacgg taagcatcag cgggtgacaa aacgagcatg cttactaata
     1321 aaatgttaga ccttcaggag gtgataggtg agttacagaa ataaaacgta cgtcatcttt
     1381 gatggcgatg aagacatgtg ggcataccga tacatgcgtg gctggaaagc taacgaaaat
     1441 attgatttca acttctttga tgctcatgat ttgaagccgc ttacagatcg tgcaggagaa
     1501 gatacggtta agaggcggct gagggaaagg cttggggata ccaagcaggc catagttctt
     1561 attggcgaaa aaacaaaaaa tctgtatcga tttgtgcgct gggaacttga aacatgcatg
     1621 aacctggata taccaatcat agctgttaat ttaaatggac aaagaagcca ggacgagaat
     1681 ctttgtcctc caataattcg tgatgagtat gtagtccaca tcccgttcaa gctaaaaata
     1741 attcagtatg ccttggacaa ctttccagga gagttccatc ggcgcaatct aagcgataaa
     1801 ggaccaaggc tatacaacga ctccgtctac aagcagctgg ggatcgaatg aaaaacgacg
     1861 aataccctgc gctgtactgc gccaccaaca aggcatcaat ggatgcccaa aaccagtact
     1921 taaagttcgt taaagtttat tctcttcttc ttattgctgc ggcagggttg ggcgtttacg
     1981 gaataaatga aaaagcatct gcggtcgcag ccgccttgct cgttatagga tctatattcc
     2041 tatctgtaat gatgctgctg agacgagatg aagatacatg gtatcgggca cgctctgtcg
     2101 ccgaatccgt taaaacaagc tcttggcgtt tcatgatgag gtgcgagcct tacgttgacg
     2161 cccctgatgt aagagtagtg aagtcaaatt tcaggacacg actgaaaagt atcttgagtg
     2221 agcataagga cttggcggag catctaggag gttcggtgtc tgaacaagag cagattaccg
     2281 acaagatgtg cgaagtaaga aatctatcgt gggagcagag ggctgacttt tatcgcacgc
     2341 atagaattga cgagcaacgt tcttggtatg caaacaagtc tgcttggaac cgcaaaaaag
     2401 gaaggctgtg gtttgctgtt ttaatcggct gccaagcttt tgccgttttg ttcctaattt
     2461 tgcgtgttgc ttatccggat tggggatatt ggcctgcgga tgtattcgtt gttgcggctg
     2521 gatcggcttt aacctggata caggtgaaac ggtttaagga gctagcagct gcatatggat
     2581 tgacagcgca cgagattgga gttgtgagag gtgagctaga gcaaatcgac tctggagaaa
     2641 agttggcgca gtttgtagct gatagcgaga acgcattttc tagagagcac actcaatggc
     2701 tagctcgcaa agactctata tagggtctaa caatgcgctg ctgtcggaca atttttccgc
     2761 tgcgctccaa aattgccgca gagcgcggcg ttatgcagtc caatgtctga attaaggaac
     2821 taaaaatgag cacacgccaa atccatgtat tcatcagtca tgcatggaaa tattctggcc
     2881 attatcaaac gcttgctgat tggattttta atcagaattg gagcgtgggg caagcatctc
     2941 tggattttag aaattattcg gttcctaaag atgatccaat tcatgatgcg ccaaatgata
     3001 ggcagcttag agatgcgatt atgaggcaga tatccatgag tcatgtggta gttattccta
     3061 cagggatgta caccaactac agcaaatgga tcgcaaaaga aattgaaggt tcgactggtt
     3121 ttaacaaacc tattcttgct gttaacccct ggggacagca aagaacgtca agcgtagttg
     3181 cgaatgcggc agcaaagata gtcggatgga ataagcagtc agtagtagat ggtatctggg
     3241 agttatacaa atagtgagta agttagaaat aacctcagat gagctaccgg gtctgtacca
     3301 atcagcaaat caagcatctc tcaacgcaca ggataactac ttcagaggct tgcgatggta
     3361 tcttatcctt ttggtatgcg cggcatttat ttcatatgcc atgccgagag atgcattggg
     3421 cgcgctgctg tcagcgggac ttttcctagt tacattagga attcttattt tcattcgtgt
     3481 acagcgcccc gatgacacat ggtataacgg gcgggcggtt gctgaatcag tgaaaacaag
     3541 aagctggcgg tggatgatga gggctgagcc gtatgaagac tgtgagagta tggaaattgt
     3601 tgctaagcag tttatcaacg acctgaagac aatcttggag caaaacaaaa gcctttctca
     3661 ctcgctgcaa tcaacaagtg ccgccaaaga ccctatctcg cagacgatga aggatgttcg
     3721 ctcaagaaac gttaaagatc gcttgtcgat ctacatcgat caaagagtgc agaatcaagt
     3781 tgaatggtac tggcacaagg ctcgctttaa caagcgaagg gcgcaacagt ggttttgggt
     3841 ctcggtgatt ctgcatgcgc ttgccattgc catgttattg tatcgcatta aggatccttc
     3901 tttttcgctt cctgtagagg ttattgcaac aggtgcgggg gctgcattaa cttggcttca
     3961 agcgaaaaag cacaacgaat taaactctgc atatgcttta acggcgcatg aaattgtatt
     4021 aattaagggc gagtcggatt ctgtccacga cgaaaagcaa ctgtctgagt atgtaattaa
     4081 tagcgaggct gcattttcgc gcgagcacac tcaatgggtc gctcgaaagg gcgattaatg
     4141 cataacaatg cgctgctgtc ggacaaattt tacgctgcgc tccaaatttg ccgcagagcg
     4201 cggcgttata gctcaagcag ctcatctcga aacgtgaagg ttttatggac gaaatagaat
     4261 tagattttgg cgcgataacc atagataacg cgacctataa gggcgaaggt tatagatttt
     4321 atgagggctt acttgctcag atgcgccaat ttaaagaaag cccagttaaa gttcttcaaa
     4381 ccgatatagt gcacaatgaa gcgatcaatc atatcgggca agagatatca aaaacccgat
     4441 cctcgattga gcaggcacta aggtcagcaa ataaacagct aaagataaaa tccgaagtta
     4501 tagaaaatgc tcgagcgttg ctatcagttg atggcagcga agctgaaata gcggaagcga
     4561 ggctggaaaa atattatgaa tttattggag ctgagaagat cgattctgga agatatgcag
     4621 atctatctcg tcttatggaa atgtattttg ctacagaagc tccttttgaa tccggaaagg
     4681 acaaaaaaaa tgaatttcca gatgcgattg cactattagc ccttgaaggt tgggctgaag
     4741 aaaatgaaat taatattatc gccgtaagtc aagataaggg ctggaagaac ttttctgaag
     4801 gttcagatag gattacgttg gtatcctctc tagcggaagc gctagaaaag tttcagccac
     4861 attacaaggt tgtaagtatt atctctcata tcagagagga ctctcttctt gatggcgaga
     4921 atcatgtact tgaagaaatt gagcaagcaa ttatcaacag cgtcgatggt tgcgatattt
     4981 gggttgaagc aagttcatat atgcactttg aatgggaaga cgtttcagcc tcctatattt
     5041 cccatgagct ggataaagat caagatggct tagttaaagt cagagttgta agtataaatg
     5101 atgaggaaat agttcttaaa gttggtgcta cagttgaagt agaggttgag gctagctttg
     5161 atttctcggt aagagattcg atagataaag attatgttgg tatgggtgga aatgtttgta
     5221 ctacaacgga gtcttatcat actgacatat tgttatctct cactggagat ttttctcagg
     5281 actttgacga catcgatgtt gctgagattg aagttcttga aactattggg cgcgcagact
     5341 tcggcgaagt tgagcctgat tggcggagtg agtacgaaga tgaagagcta taacaaggcg
     5401 ctgctacgga aaatttactc gctggcgctc ctaaatttcc gcagaacgcg gcgttatgtg
     5461 taaaaggaga tcacagtgac tctattaaaa attatcgctg tagcaaccat gctattggtt
     5521 ttggctggtt gctcaaccac caagacaatg aatggaatta tgtcttcttg ggaaggctca
     5581 aatataaatg aggttgtagc tcaatggggc tacccacatg aagaaagaga gttccaaggc
     5641 cgaaaacttt atatctggca ccacaataaa tctgcctata taccacaaac aacaaataca
     5701 actgggtcgg tatatggcaa ctctgtatat gcaacttcta ctacatcggg tggttacgcc
     5761 attcaaggca gttgccaaag ggttctggaa gttgacagtt ctggtacggt cgtgcgctgg
     5821 gaatggcgtg gcaacaactg tccattcggt gagctaatgg agtacgcaaa ttggcgcaaa
     5881 aaagaaccgg ctcagtaaca cataacaagc aaaggcagca tcgccacttc gtggctggac
     5941 gcgctaacgc gcgccgctgc tttgggcgtt aggg
//
