LOCUS       In1886                 16169 bp    DNA     linear       03-AUG-2023
DEFINITION  Pseudomonas aeruginosa strain NY5085 isolate NY5085.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Unclassified.
REFERENCE   1  (bases 1 to 16169)
  AUTHORS   Zhou,D.
  TITLE     Compelete sequence of Tn6502
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16169)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2023) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..16169
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="NY5085"
                     /isolate="NY5085"
                     /isolation_source="Hubei"
                     /host="Pseudomonas aeruginosa"
                     /country="China"
     repeat_region   complement(1..25)
                     /gene="IRt_In1886"
     CDS             142..1821
                     /gene="tniA"
                     /codon_start=1
                     /transl_table=11
                     /product="DD(35)E transposase TniA"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVVTLEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLV
                     DFLPILRRTLTRTGFVIDHIHYYADALKPWIARRERWPSFLIRRDPRDISRIWVLEPE
                     GQHYLEIPYRTLSHPAVTLWEQRQALAKLRQQGREQVDESALFRMIGQMREIVTSAQK
                     ATRKARRDADRRQHLKTSARPDKPVPPDTDIADPQADNLPPAKPFDQIEEW"
     CDS             1824..2732
                     /gene="tniB"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein TniB"
                     /translation="MDEYPIIDLSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVE
                     ALNRLEALYAWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQ
                     MPSEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAG
                     NSVNRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDD
                     CCSLLASFAASLPLRRPSPIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEA
                     INHRTLSMAVYTGPSERRRQFERELM"
     CDS             2729..3946
                     /gene="tniQ"
                     /codon_start=1
                     /transl_table=11
                     /product="Transposition auxiliary protein TniQ"
                     /translation="MKPAPRWPLHPAPKEGEALSSWLNRVALCYHMEEPDLLEHDLGH
                     GQVDDLDTAPPLSLLALLSQRSGIELDRLRCMSFAGWVPWLLDSLDDQIPDALETYAF
                     QLSVLLPRLRRKTRSITSWRAWLPSQPINRACPLCLSDPENQAVLLAWKLPLMLSCPL
                     HGCWLESYWGVPGRFLGWENADAEPRTASDAIAAMDQRTWQALTTGHVELPRRRIHAG
                     LWFRLLRTLLDELNTPLSACGTCAGYPRQVWEGCGHPLRAGQSLWRPYETLNPIVRLQ
                     MLEAAATAISLIEVRDISPPGEQAKLFWSEPQTGFTSGLPTKAPKPEPINHWQRAVQA
                     IDEAIIEARHNPETARSLFALASYGRRDPASLERLRATFVKEGIPPEFLSHYLPDAPF
                     ACLKQNDGLSDKF"
     misc_recomb     complement(3969..3987)
                     /gene="Δres"
     CDS             3998..4612
                     /gene="tnpR_Tn5393c"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn5393c resolvase"
                     /translation="MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDDLASGGRD
                     DRPGLTACLKSLRDGDVLVVWKLDRLGRSLAHLVNTVKELSDRKIGLRVLTGKGAQID
                     TTTASGRMVFGIFATLAEFERDLIRERTMAGLASARARGRKGGRKFALTKAQVRLAQA
                     AMAQRDTSVSDLCKELGIERVTLYRYVGPKGELRDHGKHVLGLT"
     CDS             4678..5481
                     /gene="strA"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptomycin resistance protein A"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     CDS             5481..6317
                     /gene="strB"
                     /codon_start=1
                     /transl_table=11
                     /product="Streptomycin resistance protein B"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     repeat_region   6343..6423
                     /gene="IRR_Tn5393c"
     CDS             6424..6900
                     /codon_start=1
                     /transl_table=11
                     /product="Probable DNA invertase"
                     /translation="MAAETLYREGRLSVSAIGEKLHISKSTLYSYLRHRGVEIGAYQK
                     SARSRDQQPSAASPAEPPAAERVATVTLRLAVVNNSKFVRGRKRATENIERYCLEPYG
                     MKRLDAGHYELTIPYRSDDELDKSVHDLLTEISQEADMRNCFVEMGAWEEDTEKRW"
     CDS             6975..7835
                     /gene="aacC2"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside N(3')-acetyltransferase III"
                     /translation="MHTRKAITEALQKLGVQTGDLLMVHASLKAIGPVEGGAETVVAA
                     LRSAVGPTGTVMGYASWDRSPYEETLNGARLDDEARRTWLPFDPATAGTYRGFGLLNQ
                     FLVQAPGARRSAHPDASMVAVGPLAETLTEPHELGHALGEGSPVERFVRLGGKALLLG
                     APLNSVTALHYAEAVADIPNKRWVTYEMPMLGRDGEVAWKTASDYDSNGILDCFAIEG
                     KPDAVETIANAYVKLGRHREGVVGFAQCYLFDAQDIVTFGVTYLEKHFGTTPIVPPHE
                     AVERSCEPSG"
     CDS             7848..8390
                     /gene="tmrB"
                     /codon_start=1
                     /transl_table=11
                     /product="Tunicamycin resistance"
                     /translation="MIIWINGPFGAGKTTLAKRLRDRRSKSLIFDPEEIGFVVKETVP
                     MPASGDYQDLPLWRGLTIAAVREIRRNYSQDIIIPMTLVHPDYLTEILDGVRRIDDQL
                     LHIFLTLNEDLLRHRIANQTMHPDPNRNAEIREWRLANVARCLAARERLPCTTRVLDS
                     GAHTSDELAAMVLDGIDGRT"
     CDS             complement(8872..9063)
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MAIALMGAGFSATDTSDAVNILYPITMTVQANKAWQASGLKKSF
                     FLPSHKKVRGGEKISYARL"
     CDS             9069..9314
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MQAYRAMVNSYSLSDDSGVMAAAAITHFLFGQAVFSYLNGWSVL
                     IGPGTGLDSTGCKYARDLMGLVAFTAFIVTFLFRGYS"
     CDS             9344..10501
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MTVRKDPVSQLSQLRSPAAVQAAIDEFVQLGRTKFLARHGYGKS
                     RDFLVRDPKTGTDCDSKAIAGVAFGKQFPEQGPLTADSFSGGEATVVPALTRLGFRII
                     RIGEDWSEEEVLATVEDYFDMLRAEAAGEPYNKSEHNQALRQLLNGRSKSSVELKHQN
                     ISAVLDALGLPYINGYKPRGNSQLLLRKSVHAYVLEHQQTVGALVDALEEVKLPGDKT
                     YRAALVEPPAREVLVRTPASLRQRLPRKFDYAARDEANRKLGRAGEQWVIGYEQQRLT
                     ELGHPELFQRLDWVSDTQGDGAGFDILSFEEDAHERFIEVKTTNGGVGSSFLVSHNEL
                     EFSKEAGDQFHLYRVFQFRDGPRLFTLPGDLSQHVHLKPTDYRASFRSLVG"
     repeat_region   10535..10559
                     /gene="IRL_Cfr1"
     CDS             10851..11987
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCfr1 transposase"
                     /translation="MCEEVHLNLAYRWFCRLGLEDEVPNHSTFSKNRHGRFRDSDLFR
                     WLFNEVLRRCMAAGLVKGEGFAVDASIIKADASRQRGVAGDEVDWNDPKLSSRAVREY
                     LEALDEEALAEALPKKISLTDPQSRWTAAPGGPAFFAYSTNYLIDTEHGVIMDVEATP
                     AHRTAEVDSTRTMVERVEAQFDLTPERLIGDTAYGTAPMLAWMVEEKDIEPHVPVWDK
                     TERKDDSLSSNDFHWSQDANEYRCPAGKPLRSEWRAFTQQRSRVTKAKTVIYRSSQTD
                     CATCPLKAKCCPNTPNRKIVRSIHEAARDVARRIAKTPEYLVSRCERKKVEMLFAHLK
                     RIMKLDRLRLRGLTGATDEFTLAAMVQNLRRMAKLLPQGPPLTG"
     repeat_region   12128..12152
                     /gene="IRR_Cfr1"
     CDS             12627..13196
                     /gene="ΔtniR"
                     /codon_start=1
                     /transl_table=11
                     /product="Truncated Serine resolvase TniR"
                     /translation="MQRDALIAAGVSLAHLYEDLASGRRDDRPGLAACLKALREGDTL
                     IVWKLDRLGRDLRHLINTVHDLTARSVGLKVLTGHGAAVDTTTAAGKLVFGIFAALAE
                     FERELISERTVAGLISARARGRKGGRPFKMTAAKLRLAMASMGQPETKVGDLCEELGI
                     TRQTLYRHVSPKGELRPDGVKLLSLGSAA"
     misc_recomb     complement(13293..13364)
                     /gene="attC_aacA4'"
     CDS             complement(13359..13913)
                     /gene="aacA4'"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 6'-N-acetyltransferase AacA4'"
                     /translation="MTNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLA
                     DVQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGWWEEETDPGVRGIDQS
                     LANASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFERQGTV
                     TTPDGPAVYMVQTRQAFERTRSDA"
     misc_recomb     complement(13932..14058)
                     /gene="attC_blaIMP-1"
     CDS             complement(14061..14801)
                     /gene="blaIMP-1"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase"
                     /translation="MSKLSVFFIFLFCSIATAAESLPDLKIEKLDEGVYVHTSFEEVN
                     GWGVVPKHGLVVLVNAEAYLIDTPFTAKDTEKLVTWFVERGYKIKGSISSHFHSDSTG
                     GIEWLNSRSIPTYASELTNELLKKDGKVQATNSFSGVNYWLVKNKIEVFYPGPGHTPD
                     NVVVWLPERKILFGGCFIKPYGLGNLGDANIEAWPKSAKLLKSKYGKAKLVVPSHSEV
                     GDASLLKLTLEQAVKGLNESKKPSKPSN"
     misc_recomb     complement(14811..14873)
                     /gene="attI1"
     CDS             14954..15967
                     /gene="intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="Integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     repeat_region   complement(16145..16169)
                     /gene="IRi_In1886"
ORIGIN      
        1 tgtcattttc agaagacgac tgcaccagtt gattgggcgt aatggctgtt gtgcagccag
       61 ctcctgacag ttcaatatca gaagtgatct gcaccaatct cgactatgct caatactcgt
      121 gtgcaccaaa gcgaggtgag catggcgacg gacaccccac ggattccaga acaaggcgtg
      181 gccactctgc ctgatgaggc ttgggagcgt gcgcgccgtc gtgcggagat catcagtccg
      241 ttggcgcagt cggagacggt cgggcacgaa gcggccgata tggcggctca ggcgctgggc
      301 ttgtctcggc gccaggtata cgttctgatc cggcgtgccc ggcaaggcag cggcctcgtg
      361 acggatctgg tgcccggcca gtccggtgga ggtaaaggta aggggcgctt gccggaaccg
      421 gtcgagcgcg tcatccacga gctactgcaa aagcggttcc tgaccaagca gaagcgcagc
      481 ctagcggcct ttcaccgcga agtcactcag gtgtgcaagg ctcaaaaact gcgagtgccg
      541 gcgcgcaata ccgtggcctt acggatcgct agccttgacc cgcgcaaggt catccgccgg
      601 cgggaaggcc aggatgccgc tcgtgaccta caaggtgtgg gcggcgagcc tcctgccgtg
      661 accgcgccgc tggagcaggt gcagatagac catacggtca tcgacctgat cgtggtcgat
      721 gaccgcgacc ggcaacctat tggccgcccg tacctgaccc tcgccatcga cgtgttcacc
      781 cgctgcgtgc tcggcatggt cgtcacgctg gaagcgccgt ctgccgtttc ggttggcctg
      841 tgcctcgtgc atgtcgcctg cgacaagcgc ccttggctgg aaggactgaa cgtggaaatg
      901 gattggcaga tgagcggcaa gcccttgctg ctctacctag acaacgcggc cgagttcaag
      961 agcgaggccc tgcgccgggg ttgcgagcag catggcatcc ggctggacta tcgcccgctg
     1021 ggacagccgc actatggcgg catcgtggaa cggatcatcg gcacggcgat gcagatgatt
     1081 cacgacgaac tgccgggaac gaccttctcc aaccctgacc agcgcggcga ctacgattcc
     1141 gaaaacaagg ccgccctgac gctgcgcgag ctagagcgct ggctcacatt ggcggtcggc
     1201 acctaccacg gttcggtgca caacggcctg ctccaaccgc cggccgcgcg ctgggccgag
     1261 gccgtggcgc gtgtcggcgt accggccgtc gtcacacgcg ctacttcgtt cctggtcgat
     1321 tttctgccga tcctccggcg cacgctgacc cgcaccggct ttgtcatcga ccacatccac
     1381 tactacgccg atgcgctcaa gccgtggatt gcgcggcgtg aacgctggcc gtcctttctg
     1441 atccggcgcg atccgcgcga catcagccgt atctgggtcc tggaaccgga gggacagcat
     1501 tacctggaaa ttccctaccg taccttgtcg catccggctg tcaccctctg ggaacaacgg
     1561 caggcgctgg cgaaactgcg gcagcaaggg cgcgaacagg tggatgagtc ggcgctgttc
     1621 cgcatgatcg gccagatgcg tgagattgtg accagcgcgc agaaggccac acgcaaggcg
     1681 cggcgtgacg cggatcgccg ccagcacctc aagacatcag ctcggccgga caagcccgtt
     1741 ccgccggata cggatattgc cgacccgcag gcagacaact tgccacccgc caaaccgttc
     1801 gaccagattg aggagtggta gccgtggacg aatatcccat catcgacctg tcccacctgc
     1861 tgccggcggc ccagggcttg gcccgtcttc cggcggacga gcgcatccag cgccttcgcg
     1921 ccgaccgctg gatcggctat ccgcgcgcag tcgaggcgct gaaccggctg gaagcccttt
     1981 atgcgtggcc aaacaagcaa cgcatgccca acctgctgct ggttggcccg accaacaatg
     2041 gcaagtcgat gatcgtcgag aagttccgcc gcacccaccc ggccagctcc gacgccgacc
     2101 aggagcacat cccggtgttg gtcgtgcaga tgccgtccga gccgtccgtg atccgcttct
     2161 acgtcgcgct gctcgccgcg atgggcgcgc cgctgcgccc acgcccacgg ttgccggaaa
     2221 tggagcaact ggctctggca ctgctgcgca aggtcggcgt gcgcatgctg gtgatcgacg
     2281 agctgcacaa cgtgctggcc ggcaacagcg tcaaccgccg ggaattcctc aacctgctgc
     2341 gcttcctcgg caacgaactg cgcatcccgt tggttggggt aggcacgcgc gacgcctacc
     2401 tagccatccg ctccgatgac cagttggaaa atcgcttcga gccgatgatg ctgccggtat
     2461 gggaggccaa cgacgattgc tgctcactgc tggccagctt cgccgcttcg ctcccgctgc
     2521 gccggccttc cccaattgcc acgctggaca tggctcgcta cctgctcaca cgcagcgagg
     2581 gcaccatagg ggaactggcg cacttgctga tggcggcggc catcgtcgcc gtggagagcg
     2641 gcgaggaagc gatcaaccat cgcacactca gcatggccgt ttacaccgga cccagcgagc
     2701 ggcggcggca attcgagcgg gaactgatgt gaagcctgcg ccgcgctggc cgctgcatcc
     2761 cgccccgaaa gaaggcgagg cgctgtcctc atggctcaac cgcgtggccc tttgctatca
     2821 catggaggag cccgacctgc tggagcacga tcttggtcac ggccaggtcg atgacctgga
     2881 caccgcgcca ccactctcgc tgctggcgtt gctttcccag cggagcggca tcgagctgga
     2941 ccggctgcgc tgtatgagtt tcgccggatg ggtgccttgg ctactggaca gccttgatga
     3001 ccagattcca gacgccttgg aaacctatgc gtttcagctc tcggtgttgc tgccaagact
     3061 ccgccgtaag acgcgatcca tcacgagctg gcgtgcctgg ctgcccagcc agccgataaa
     3121 ccgcgcctgt ccgctctgcc tgagcgatcc ggagaaccaa gccgtactgc tcgcgtggaa
     3181 gctgcccctg atgctgagct gcccgctgca tggctgctgg ctggaatcct attggggcgt
     3241 gccagggcgg tttctcggct gggagaacgc cgacgccgaa ccgcgcaccg ccagcgacgc
     3301 gattgcggcg atggaccagc gtacctggca ggcactgaca accggtcacg tggagctgcc
     3361 gcgccgacgc atccacgccg gattgtggtt tcgacttctt cgcacgctgc tcgatgagct
     3421 gaacaccccg ctttccgcgt gcggaacctg cgcggggtat ccccgccaag tctgggaagg
     3481 ctgcgggcat ccgctgcgtg ctgggcaaag tctgtggcga ccgtatgaaa ccctgaatcc
     3541 gatagtacgg ttacagatgc tggaggcggc ggcaacggca atcagcttga ttgaggtgag
     3601 ggacatcagc ccgccaggcg agcaggcaaa gctattctgg tccgagcccc aaaccgggtt
     3661 caccagtggc ctgccgacga aagcgccgaa gcccgagccc atcaatcact ggcagcgtgc
     3721 agtccaggcc atcgacgagg ccatcattga agcgcgacac aaccccgaga cggcacgctc
     3781 gctgttcgcg ttggcttcct atggtcggcg cgatcccgct tccttggaac ggttgcgcgc
     3841 caccttcgtg aaggaaggca tcccgccgga atttctgtca cattacctgc ctgatgcacc
     3901 ctttgcatgt cttaaacaaa atgacgggtt aagtgacaaa ttttgacgga tagagctttc
     3961 cggctcacac tgtcacataa tcgaacgttt gtgatacatg ctgatcggat atgcccgcgt
     4021 ctccaaagcc gatggctcgc agtctctcga cctgcagcac gacgccttgc gcgccgcagg
     4081 tgtcgaacgg gacaatatct atgatgatct tgcttccggc ggtcgtgatg atcgccctgg
     4141 cttgactgcc tgcctcaagt cattgcgtga cggcgatgtg ctggtggtct ggaagctcga
     4201 tcgcctcgga cgatcgcttg cccatctggt caacacggtg aaggagctgt cagaccgcaa
     4261 gatcggcctg cgggttctga ctggaaaggg cgctcagatc gacaccacga ctgcgtccgg
     4321 tcgcatggtg ttcggaatct tcgccacctt ggccgagttc gagcgggatc tgatccgaga
     4381 gcgcaccatg gcgggtctcg cctccgcgag agcgcgcggt cgcaagggcg gacgaaaatt
     4441 cgcgctcacc aaagctcagg tgcgtctcgc gcaagccgcc atggcccagc gcgatacttc
     4501 agtttccgat ctctgcaagg aactcggcat cgagcgcgtc actctctacc gatatgtcgg
     4561 tcccaaaggc gagctcagag accatggaaa gcatgttctc ggacttacgt agcaactcgt
     4621 ttcttttcgc aggttgagcc acctccgcgc ttcatcagaa aactgaagga acctccattg
     4681 aatcgaacta atattttttt tggtgaatcg cattctgact ggttgcctgt cagaggcgga
     4741 gaatctggtg attttgtttt tcgacgtggt gacgggcatg ccttcgcgaa aatcgcacct
     4801 gcttcccgcc gcggtgagct cgctggagag cgtgaccgcc tcatttggct caaaggtcga
     4861 ggtgtggctt gccccgaggt gatcaactgg caggaggaac aggagggtgc atgcttggtg
     4921 ataacggcaa ttccgggagt accggcggct gatctgtctg gagcggattt gctcaaagcg
     4981 tggccgtcaa tggggcagca acttggcgct gttcacagcc tatcggttga tcaatgtccg
     5041 tttgagcgca ggctgtcgcg aatgttcgga cgcgccgttg atgtggtgtc ccgcaatgcc
     5101 gtcaatcccg acttcttacc ggacgaggac aagagtacgc cgcagctcga tcttttggct
     5161 cgtgtcgaac gagagctacc ggtgcggctc gaccaagagc gcaccgatat ggttgtttgc
     5221 catggtgatc cctgcatgcc gaacttcatg gtggacccta aaactcttca atgcacgggt
     5281 ctgatcgacc ttgggcggct cggaacagca gatcgctatg ccgatttggc actcatgatt
     5341 gctaacgccg aagagaactg ggcagcgcca gatgaagcag agcgcgcctt cgctgtccta
     5401 ttcaatgtat tggggatcga agcccccgac cgcgaacgcc ttgccttcta tctgcgattg
     5461 gaccctctga cttggggttg atgttcatgc cgcctgtttt tcctgctcat tggcacgttt
     5521 cgcaacctgt tctcattgcg gacacctttt ccagcctcgt ttggaaagtt tcattgccag
     5581 acgggactcc tgcaatcgtc aagggattga aacctataga agacattgct gatgaactgc
     5641 gcggggccga ctatctggta tggcgcaatg ggaggggagc agtccggttg ctcggtcgtg
     5701 agaacaatct gatgttgctc gaatatgccg gggagcgaat gctctctcac atcgttgccg
     5761 agcacggcga ctaccaggcg accgaaattg cagcggaact aatggcgaag ctgtatgccg
     5821 catctgagga acccctgcct tctgcccttc tcccgatccg ggatcgcttt gcagctttgt
     5881 ttcagcgggc gcgcgatgat caaaacgcag gttgtcaaac tgactacgtc cacgcggcga
     5941 ttatagccga tcaaatgatg agcaatgcct cggaactgcg tgggctacat ggcgatctgc
     6001 atcatgaaaa catcatgttc tccagtcgcg gctggctggt gatagatccc gtcggtctgg
     6061 tcggtgaagt gggctttggc gccgccaata tgttctacga tccggctgac agagacgacc
     6121 tttgtctcga tcctagacgc attgcacaga tggcggacgc attctctcgt gcgctggacg
     6181 tcgatccgcg tcgcctgctc gaccaggcgt acgcttatgg gtgcctttcc gcagcttgga
     6241 acgcggatgg agaagaggag caacgcgatc tagctatcgc ggccgcgatc aagcaggtgc
     6301 gacagacgtc atactagata tcaagcgact tctcctatcc cctgggaaca catcaatctt
     6361 accggagaat atcgttggcc aaagccttag cgtaggattt cgccctctcc cgcaaacgac
     6421 cccatggcgg ccgaaaccct ctaccgcgaa ggtcgcctga gcgtcagcgc gatcggcgag
     6481 aagctgcaca tctccaagag cacgctgtac agctacctgc gccaccgtgg tgtcgagatc
     6541 ggcgcgtacc agaagagcgc caggtcacgc gaccagcagc cttcggccgc gtcgccggca
     6601 gagccgcccg ccgccgagcg ggtggccacc gtcaccctgc gcctcgcggt ggtgaataac
     6661 agcaagttcg tgcgcggccg gaagcgggcc acggagaaca ttgagcgcta ctgcctggag
     6721 ccctatggca tgaagcggct ggatgccggc cactatgagt tgaccattcc gtatcggagc
     6781 gacgatgagc tggacaagag cgtgcatgac ctgctgaccg agatcagcca ggaggccgac
     6841 atgcgcaact gttttgtcga gatgggcgcc tgggaagaag acaccgaaaa gcgttggtag
     6901 ggcttacgtt ggtttttggt tccattgctc cccaaacccc gtatagtgtt ttgcagttta
     6961 gaggagatat cgcgatgcat acgcggaagg caataacgga ggcgcttcaa aaactcggag
     7021 tccaaaccgg tgacctcttg atggtgcatg cctcacttaa agcgattggt ccggtcgaag
     7081 gaggagcgga gacggtcgtt gccgcgttac gctccgcggt tgggccgact ggcactgtga
     7141 tgggatacgc gtcgtgggac cgatcaccct acgaggagac tctgaatggc gctcggctgg
     7201 atgacgaagc ccgccgtacc tggctgccgt tcgatcccgc aacagccggg acttaccgtg
     7261 ggttcggcct gctgaatcaa tttctggttc aagcccccgg cgcgcggcgc agcgcgcacc
     7321 ccgatgcatc gatggtcgcg gttggtccgc tggctgaaac gctgacggag cctcacgaac
     7381 tcggtcacgc cttgggggaa ggatcgcccg tcgagcggtt cgttcgcctt ggcgggaagg
     7441 ccctgctgtt gggtgcgccg ctaaactccg ttaccgcatt gcactacgcc gaggcggttg
     7501 ccgatatccc caacaaacgg tgggtgacgt atgagatgcc gatgcttgga agagacggtg
     7561 aagtcgcctg gaaaacggca tcggattacg attcaaacgg cattctcgat tgctttgcta
     7621 tcgaaggaaa gccggatgcg gttgaaacta tagcaaatgc ttacgtgaag ctcggtcgcc
     7681 atcgagaagg tgtcgtgggc tttgctcagt gctacctgtt cgacgcgcag gacatcgtga
     7741 cgttcggcgt cacctatctt gagaagcatt tcggaaccac tccgatcgtg cctccgcacg
     7801 aggccgtcga gcgctcttgc gagccttcag gttagaggcc gtcgacaatg ataatctgga
     7861 tcaacggacc tttcggcgcc ggaaagacga cgctcgctaa gcggctgcgc gatcggcgtt
     7921 ccaaatcgct gatctttgac cccgaggaaa tcgggttcgt ggtgaaagaa acggtcccca
     7981 tgccagcgag cggagactat caggatctcc ccttgtggag gggacttacg atcgcggcgg
     8041 tcagggagat tcgaaggaat tactcgcagg acatcatcat cccaatgacg ctcgtgcacc
     8101 cggactatct gactgagata ctcgacgggg taaggcggat cgacgatcag ctgctgcaca
     8161 tctttctgac gctcaacgag gacctattgc gtcaccggat cgcgaaccag accatgcatc
     8221 ctgacccgaa tcgaaatgcg gagattcgag agtggcgatt agcgaatgtc gcccgatgct
     8281 tggccgcaag ggaacggctt ccatgcacaa cccgtgttct cgatagtggt gcacacacca
     8341 gcgatgaact cgcagcgatg gtgctcgacg gaatcgatgg gcgcacctga tcgccttcga
     8401 cgcctgcgca aagcgtagcg cgagggtggc gggctcacga ccaaacgccc agaggtcgat
     8461 catcgcaggg atgtttggct ttgtggtgcg gacgacggga ctcgaacccg tactctcaca
     8521 gagaagcaga ttttcgtacc acctcgactt tcgccgccgt ctgatgacgt tcgtggtctg
     8581 gactgtccct tcgccattgc ccgaaggctt taggcgccgc ccgtccagtc tctacacctt
     8641 cccccgaagg ggcttggctc gggattggct tagggtattg cccgttagcg ttccccgact
     8701 ttgagcggtt ctactccgcg gatttccccg cgggcactcc aattttaaag tctgctgcgt
     8761 ctaccgattt cgccacgtcc gccttttttc gccgttccta gcgctcgtgc gatgcaccta
     8821 tgttgcacct agcgccgaat cgttcttcgt catcctgaaa aaccacgtct cctaaagcct
     8881 tgcatagctt atcttttctc caccacgaac ttttttgtgg gatggtagaa aaaaagactt
     8941 tttaagtccg ctggcttgcc aggccttgtt agcttgtacg gtcatggtta tcgggtaaag
     9001 aatattgacg gcatcgctgg tgtcggtggc tgaaaagccg gctcccatca gggcaatagc
     9061 catttcagat gcaggcgtac agggcaatgg tcaacagcta cagcctgtct gacgattccg
     9121 gcgtcatggc tgcggcggct atcacgcatt ttttgttcgg tcaggcggtg ttttcgtacc
     9181 tcaatggttg gagcgtgttg atcggacctg gtacaggttt ggacagcacg ggctgcaaat
     9241 acgcaaggga tttaatgggc ctggtggcgt tcacggcttt tatcgtgacg tttctgttca
     9301 ggggctactc ataatctcgt ggctcggcgg ttcccggcac accatgacag taaggaagga
     9361 ccctgtgtct caactctccc agcttcgaag ccccgccgcc gtgcaggctg ccatcgatga
     9421 gttcgtgcaa ctgggccgca cgaaattcct ggcgcgccac ggctacggca agtcccgcga
     9481 cttcctggta cgtgatccga agaccggcac cgattgcgat tccaaggcca tcgccggtgt
     9541 ggccttcggc aagcaatttc ccgagcaggg cccgctcact gctgacagct tctccggtgg
     9601 cgaggcgacc gtcgttccgg cgctgacgcg gctcgggttt cgcatcattc gcatcggcga
     9661 agactggtcc gaagaagagg tcctggccac ggtcgaagac tatttcgaca tgctgcgtgc
     9721 cgaggcggct ggggagccgt acaacaagtc cgagcacaac caggcactgc gccaactgct
     9781 gaacggtcgc agcaagtctt cagtcgagct caagcaccag aacattagcg ccgtactcga
     9841 tgccctgggc ctgccctata tcaacggcta caagccacgc ggcaacagcc aactgctgct
     9901 gcgtaaatcc gtacacgcct acgttctgga acatcagcag acggtcggcg ctcttgtcga
     9961 tgccctggag gaggtaaaac ttccgggtga caaaacctac cgagcggctt tggtagaacc
    10021 acccgcccgt gaagtgcttg tgcgtacccc ggcatctcta cggcaacgcc taccgcgaaa
    10081 gttcgattat gccgctcgcg atgaagccaa ccgcaagctg ggccgggcag gggagcagtg
    10141 ggtgattggc tacgaacagc aacgcctgac cgagctcggc cacccagagc tttttcagcg
    10201 gctggattgg gtgtccgaca cccagggaga cggtgcgggg ttcgacatcc tgtcgttcga
    10261 agaggacgcc catgagcgct tcatcgaggt gaaaaccacc aatggcgggg taggctcgtc
    10321 tttcttggtc agccacaacg aactcgaatt ctccaaggag gcgggcgatc aattccatct
    10381 gtatcgcgtg ttccagtttc gggacggtcc gcgcctgttc acgctacccg gcgacctcag
    10441 ccaacatgtg catctcaagc cgacggacta ccgggcgagt ttccggagtt tggtggggta
    10501 aaggcagggt tctgttgagc cgaatggctg tgtgcggccg attctgttga aaaagtagcg
    10561 gcctccccat gccgttggca aaattgcttt gtcagcgagc gtgggggcga acagcatgat
    10621 gggacagtta ccgggaggac agcagcgcct gttctactcg ttcaatctgg aagatcacgt
    10681 cccggcccaa catctcctgc gcagcatcga ccagtgcttg gatctcagtg atctacgtgc
    10741 ctacctggca gatttctata gccccatcgg gcgtccctcg attgacccgg agttgatggt
    10801 gcgcatgctg gtcgtcggct acttgctatg gcattcgttc cgagcggcga ttgtgcgaag
    10861 aggtgcacct gaacctggcc tatcgctggt tctgccggtt gggtctggaa gacgaagtcc
    10921 ccaatcactc gaccttctcg aagaatcgcc atgggcgttt tcgtgacagc gatctattcc
    10981 gctggttatt caatgaggtg ctgcggcgct gcatggcagc cggcctagtc aagggtgaag
    11041 gtttcgccgt cgacgccagc atcattaagg cggatgccag ccggcaacgt ggggtggcgg
    11101 gagatgaggt cgattggaac gatccaaagc tcagcagccg cgcagtgcgc gagtacctcg
    11161 aagcccttga tgaagaggcg ctggctgagg ctcttcccaa gaaaatttcg ctcactgatc
    11221 ctcagtcccg ttggacagca gcgccaggtg gcccggcctt ttttgcctac tccacgaatt
    11281 acctgatcga cactgagcac ggtgtgatca tggacgtgga agctaccccg gcgcaccgta
    11341 ccgccgaagt cgattcgact aggacgatgg tcgagcgtgt cgaggcgcag ttcgatctca
    11401 caccggaacg ccttatcggc gataccgctt atggcaccgc cccgatgctg gcctggatgg
    11461 tcgaagaaaa ggacatcgaa ccgcatgtgc cggtgtggga caagaccgag cgcaaggacg
    11521 acagcctctc cagtaacgac tttcactgga gtcaggacgc caatgaatat cgctgcccag
    11581 ccggcaaacc gctacgcagt gaatggcgcg ccttcaccca gcaaaggtcg cgggtaacta
    11641 aggccaaaac cgtcatttac cgctccagcc aaaccgactg cgccacctgc ccgttgaaag
    11701 cgaaatgctg ccccaacacg ccgaatcgga agatcgtccg cagcatccat gaggctgccc
    11761 gcgacgtggc tcgacgcatc gccaagacac cggagtacct cgtctctcgc tgcgaacgaa
    11821 agaaggtgga gatgcttttc gcccacctca aacggatcat gaaactcgac cgtttacgac
    11881 tgcgtggcct aacgggtgcc actgacgaat tcaccttggc tgcgatggtg cagaacctgc
    11941 gccgcatggc caagcttttg cctcaagggc caccgctgac gggataggta tgcctgctac
    12001 gagcagaaac cctcaaatta acccttaaac ctgagcaagg acgctcagtg aaacgccgga
    12061 aggcaacttg aagtggcttg cagccacttc gacagcaggc acacctgatc ggcaggctgc
    12121 cgctaaagct actttttcaa cagaatcggc cgatttgtgc cggtcgtcaa ggaccgcttc
    12181 ggatcactct caaccggatc gcgggccagc tgatttgcta aaacccgcgc caaactcaac
    12241 gaaggatttc gcatgatccg ttttttttgg cgatcacgct cggcctgatg gttggctgct
    12301 cgtcgagttc aagttcgatc tgatcagtga gcggtagcgc aagtggatcg cctcagccaa
    12361 gcagcgttgt tgccggcggc cggaagaaag ccttgaacta gtagcggaaa ggatgaaact
    12421 ttatttttac gattctcagc attttccaag gttgagcgaa aattaggatg atactttaat
    12481 gattccggcc tccatcccca caaaacgctt gcagatggca gaattgaaat aagtcattgt
    12541 tttaaatgga tttttgcagg aattgctacg ttgcagggtc ctgtagttag gatgacactt
    12601 tattttcctc ccacaagtcc accaatttgc aacgcgatgc gctcatcgcc gctggtgtga
    12661 gccttgcgca cctttacgag gatctggcct cgggcaggcg cgatgatcgc ccagggttgg
    12721 ctgcttgcct gaaggcgctt cgtgaagggg acacgctgat cgtgtggaag ctcgatcggc
    12781 ttggccgtga tctgcgccac ctgatcaaca ccgtgcacga cctaactgcg cgtagcgtgg
    12841 gcctgaaggt cctgaccggt cacggtgcgg cggtcgacac gacgactgcc gccggcaagc
    12901 ttgtgttcgg tatttttgcc gcgctggccg agttcgagcg tgagttgatt tccgagcgaa
    12961 cagtcgctgg acttatctcg gcgcgcgctc gcggcaggaa aggggggcgc cccttcaaga
    13021 tgaccgccgc caagctacgc ctggcgatgg ccagcatggg gcaaccggaa accaaggtgg
    13081 gcgatctctg cgaagaactc gggattaccc ggcagacgct ctaccggcac gtgtcgccca
    13141 agggcgaact gcggccagac ggcgtaaagc tgctctccct cggttcagcc gcataaatgg
    13201 aggcgacctg gaacggggcg ctgttcagtg cggcaacgat ccgattaccg gtgtcgaccc
    13261 agagcagccg tagagctttt gggaaagctg tcgttcaacg tttgacatga ggggcggcca
    13321 agggcgccag cccttggacg tccccctcga tggaagggtt aggcatcact gcgtgttcgc
    13381 tcgaatgcct ggcgtgtttg aaccatgtac acggctggac catctggggt ggttacggta
    13441 ccttgcctct caaaccccgc tttctcgtag catcggatcg ctcgcaagtt gctcggcgac
    13501 gggtccgttt ggatcttggt gacctcggga tcattgaaca gcaactcaac cagtgctcga
    13561 accagcttgg ttcccaagcc tttgcccagt tgtgatgcat tcgccagtga ctggtctatt
    13621 ccgcgtactc ctggatcggt ttcttcttcc caccatccgt ccccgcttcc aagagcaacg
    13681 tacgactggg catacccaat cggctctcca ttcagcattg caatgtatgg agtgacggac
    13741 tcttgcgcta aaacgcttgg caagtactgt tcctgtacgt cagcaagtgt cgggcgtgct
    13801 tcttctccgc cccaccactc gacgatatga gatcgattta gccactcata gagcatcgca
    13861 aggtcatgct cagtcatgag gcgcagtgtg acggaatcgt tgctgttggt cacgatgctg
    13921 tactttgtga tgcctaacgc taagttcagc ggcagtttgt aagttgcgcg ttgtggaata
    13981 ctttgcgaag caaaccacaa aagcgcaact tacaaactgt ccagccacgt agtggcgtgc
    14041 tgcaacgact tgttagaaat ttagttgctt ggttttgatg gttttttact ttcgtttaac
    14101 cctttaaccg cctgctctaa tgtaagtttc aagagtgatg cgtctccaac ttcactgtga
    14161 cttggaacaa ccagttttgc cttaccatat ttggacttta ataatttggc ggactttggc
    14221 caagcttcta tatttgcgtc acccaaattg cctaaaccgt acggtttaat aaaacaacca
    14281 ccgaataata ttttcctttc aggcagccaa accactacgt tatctggagt gtgtcccggg
    14341 cctggataaa aaacttcaat tttattttta actagccaat agttaactcc gctaaatgaa
    14401 tttgtggctt gaaccttgcc gtctttttta agcagttcat ttgttaattc agatgcatac
    14461 gtggggatag atcgagaatt aagccactct attccgcccg tgctgtcgct atgaaaatga
    14521 gaggaaatac tgccttttat tttatagcca cgctccacaa accaagtgac taacttttca
    14581 gtatctttag ccgtaaatgg agtgtcaatt agataagcct cagcatttac aagaaccacc
    14641 aaaccatgtt taggaacaac gccccacccg ttaacttctt caaacgaagt atgaacataa
    14701 acgccttcat caagcttttc aatttttaaa tctggcaaag actctgctgc ggtagcaatg
    14761 ctgcaaaaca aaaatataaa gaatacagat aacttgctca tacttttcct tttctaactt
    14821 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
    14881 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca
    14941 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
    15001 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc
    15061 ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt
    15121 gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt
    15181 ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac
    15241 ggatctgccc tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt
    15301 gctgaccccg gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt
    15361 cgcccagctt ctgtatggaa cgggcatgcg gatcagtgag ggtttgcaac tgcgggtcaa
    15421 ggatctggat ttcgatcacg gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg
    15481 ggccttgatg ttacccgaga gcttggcacc cagcctgcgc gagcagctgt cgcgtgcacg
    15541 ggcatggtgg ctgaaggacc aggccgaggg ccgcagcggc gttgcgcttc ccgacgccct
    15601 tgagcggaag tatccgcgcg ccgggcattc ctggccgtgg ttctgggttt ttgcgcagca
    15661 cacgcattcg accgatccac ggagcggtgt cgtgcgtcgc catcacatgt atgaccagac
    15721 ctttcagcgc gccttcaaac gtgccgtaga acaagcaggc atcacgaagc ccgccacacc
    15781 gcacaccctc cgccactcgt tcgcgacggc cttgctccgc agcggttacg acattcgaac
    15841 cgtgcaggat ctgctcggcc attccgacgt ctctacgacg atgatttaca cgcatgtgct
    15901 gaaagttggc ggtgccggag tgcgctcacc gcttgatgcg ctgccgcccc tcactagtga
    15961 gaggtagggc agcgcaagtc aatcctggcg gattcactac ccctgcgcga aggccatcgg
    16021 tgccgcatcg aacggccggt tgcggaaagt cctccctgcg tccgctgatg gccggcagca
    16081 gcccgtcgtt gcctgatgga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
    16141 ttcagtgcag ccgtcttctg aaaacgaca
//
