LOCUS       In1789                 15540 bp    DNA     linear   BCT 09-NOV-2020
DEFINITION  Pseudomonas aeruginosa.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 15540)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of In1789
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15540)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..15540
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
     mobile_element  1..15540
                     /gene="In1789"
                     /product="Concise class 1 integron: In1789"
     repeat_region   1..25
                     /gene="IRi_In1789"
                     /product="Inverted repeat at the integrase end of In1789"
     CDS             complement(203..1216)
                     /gene="intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="Integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     regulatory      1101..1129
                     /gene="PcWTGN-10"
                     /product="Promoter PcWTGN-10"
     regulatory      1101..1106
                     /gene="-35 region_PcWTGN-10"
                     /product="-35 region of PcWTGN-10"
     regulatory      1121..1122
                     /gene="Extended_-10"
                     /product="Extended -10 region"
     regulatory      1124..1129
                     /gene="-10 region_PcWTGN-10"
                     /product="-10 region of PcWTGN-10"
     misc_recomb     1297..1359
                     /gene="attI1"
                     /product="attI1 site"
     CDS             1377..1931
                     /gene="aacA4'-17"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 6'-N-acetyltransferase"
                     /translation="MTNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLA
                     DVQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGWWEEETDPGVRGIDQS
                     LANASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFEKQGTV
                     TTPDGPAVYMVQTRQAFERTRSDA"
     misc_recomb     1926..1997
                     /gene="attC_aacA4'-17"
                     /product="attC site for aacA4'-17"
     CDS             2061..2927
                     /gene="blaCARB-2"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-lactamase CARB-2"
                     /translation="MKFLLAFSLLIPSVVFASSSKFQQVEQDVKAIEVSLSARIGVSV
                     LDTQNGEYWDYNGNQRFPLTSTFKTIACAKLLYDAEQGKVNPNSTVEIKKADLVTYSP
                     VIEKQVGQAITLDDACFATMTTSDNTAANIILSAVGGPKGVTDFLRQIGDKETRLDRI
                     EPDLNEGKLGDLRDTTTPKAIASTLNKFLFGSALSEMNQKKLESWMVNNQVTGNLLRS
                     VLPAGWNIADRSGAGGFGARSITAVVWSEHQAPIIVSIYLAQTQASMAERNDAIVKIG
                     HSIFDVYTSQSR"
     misc_recomb     2931..3041
                     /gene="attC_blaCARB-2"
                     /product="attC site for blaCARB-2"
     CDS             3057..3836
                     /gene="aadA2"
                     /codon_start=1
                     /transl_table=11
                     /product="Aminoglycoside 3''-nucleotidyltransferase"
                     /translation="MTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKPYSDI
                     DLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYPAKRE
                     LQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQDLFE
                     ALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPAQYQP
                     VLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
     misc_recomb     3838..3897
                     /gene="attC_aadA2"
                     /product="attC site for aadA2"
     CDS             4000..4347
                     /gene="qacED1"
                     /codon_start=1
                     /transl_table=11
                     /product="Quarternary ammonium coumpound resistance
                     protein"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     misc_feature    4343..4869
                     /gene="Δsul1"
                     /product="Dihydropteroate synthase (pseudo"
     CDS             4878..5312
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MVHGETADLAPTGFGQQVREAMDQRREHHIEQRDATRNRDGRIF
                     YRRNLLATLREREVARAGAEMAEGKALPFRAAKDGESVSGKFTGTVQLTSGKFAIVEK
                     SHEFTLVPWRPIIDRQLGREVAGIMQGGSVSWQLGRQRGLGL"
     CDS             5529..6743
                     /gene="cmlA9"
                     /codon_start=1
                     /transl_table=11
                     /product="Chloramphenicol efflux protein"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGVL
                     NTTPSIIQLTLSLYMVMLGVGQVIFGPLSDRVGRRPILLVGATAFVAASLGAACSSTA
                     LAFVAFRLVQAVGASAMLVATFATVRDVYANRPEGAVIYGLFSSMLAFVPALGPIAGA
                     LIGEFWGWQAIFITLAALASLALLNASFRWHETRPLDQARTQRSVLPIFASPAFWVYT
                     VGFSAGMGTFFVFFSTAPRVLIGQAGYSEIGFSLAFATVALVMVTTTRFAKSFVAKWG
                     IAGCVARGMALLVSGAILLGIGQLFGSPSFFSFILPMWVVAVGIVFTVSVTANGALAQ
                     FDDIAGSAVAFYFCIQSLIVSIVGTLAVTLLNGDTAWPVICYATAMAVLVSLGLALLR
                     SRDAATEKSPVV"
     CDS             complement(6950..7576)
                     /gene="tetR(G)"
                     /codon_start=1
                     /transl_table=11
                     /product="Tetracycline resistance regulator protein TetR,
                     class G"
                     /translation="MTKLDKGTVIAAALELLNEVGMDSLTTRKLAERLKVQQPALYWH
                     FQNKRALLDALAEAMLAERHTRSLPEENEDWRVFLKENALSFRTALLSYRDGARIHAG
                     TRPTEPNFGTAETQIRFLCAEGFCPKRAVWALRAVSHYVVGSVLEQQASDADERVPDR
                     PDVSEQAPSSFLHDLFHELETDGMDAAFNFGLDSLIAGFERLRSSTTD"
     misc_feature    7680..8453
                     /gene="ΔtetA(G)-5'"
                     /product="Truncated Tetracycline efflux protein TetA,
                     class G, 5' fragment"
     mobile_element  complement(8454..9333)
                     /gene="IS6100"
                     /product="Insertion sequence: IS6100"
     repeat_region   complement(8454..8467)
                     /gene="IRR_IS6100"
                     /product="IS6100 inverted repeat right"
     CDS             complement(8508..9272)
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6100 transposase"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     repeat_region   complement(9320..9333)
                     /gene="IRL_IS6100"
                     /product="IS6100 inverted repeat left"
     CDS             complement(9354..9854)
                     /gene="orf5"
                     /codon_start=1
                     /transl_table=11
                     /product="Putative acetyltransferase"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     CDS             complement(9982..10833)
                     /gene="sul1"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydropteroate synthase"
                     /translation="MLRSRVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSD
                     VVDVGPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVG
                     YLNDIQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEAR
                     VSALRRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSF
                     LGATVGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDAR
                     DRGLDHA"
     misc_recomb     complement(10818..10854)
                     /gene="ΔattI1"
                     /product="Truncated attI1 site"
     misc_feature    10935..11235
                     /gene="ΔintI1"
                     /product="Truncated integron integrase IntI1 (pseudo"
     regulatory      complement(11022..11050)
                     /gene="PcH1"
                     /product="Promoter PcH1"
     regulatory      complement(11022..11027)
                     /gene="-10 region"
                     /product="-10 region of PcH1"
     regulatory      complement(11045..11050)
                     /gene="-35 region"
                     /product="-35 region of PcH1"
     mobile_element  complement(11611..13591)
                     /gene="ISCR3"
                     /product="Insertion sequence: ISCR3"
     CDS             11815..13347
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISCR3 transposase"
                     /translation="MPRLATSRRQAAGCAPLPSAHTGSRYARHAPERTLLYALVEAHY
                     PDFIARIEAEGRSLPGYVREAFDAYLRCGVLEHGFLRVVCEHCRAERLVAFSCKKRGF
                     CPSCGARRMAESARHLVEEVFGPRPVRQWVLSFPYPLRFLFASKPEAIGPVLGIVQRV
                     IAGWLADQAGIDRASAQCGAVTLIQRFGSALNLNIHFHMLWLDGVYVEATELPRRELR
                     LHRARAPTTAQLTQLAATIAHRVCRHLTRKGWLEGEGESAFLADSAAGDDSMDGLRMS
                     SITYRIATGRDAGCKVVTLQTLPGDAGSLEGEAGKVGGFSLHAGVAAEAHESHKLEKL
                     CRYITRPAISEKRLSIALQGRVRYQLKTPWRNGTTHVEWDPVDFIAKLAALVPPPRAH
                     LTRFHGVFAPNANLRAQLTPSGRGKRPAGDAAPVDVSAHDAPRSPEEKRRAMSWAQRL
                     KRVFSIDVTACVHCGGTVRIVASIEEPTAIRAILAHFEKHGAREEAHYRPAARAPPVQ
                     AA"
     CDS             complement(13439..14230)
                     /gene="lysR"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family DNA-binding transcriptional
                     regulator"
                     /translation="MRRLRRNDGLNLLSNHLLSGRVPMMTLVHTLAVAEYLNFRHAAN
                     ALGVAQSSVSARVKALEEDLGILLFERHARGVRLTEAGRHFVERIAVGIDQLDHAVKT
                     AGMAAAGESGRLRIGIHALIPHSFLAKLIGQYRKDYPDVEVEIAEGPAREAVVQLRAG
                     RLDVAFVAGTPQPPDCHSRRTWTEPLLAVLPERHPLAKRSAVTWPDLAGETFLAYRKF
                     KRPFRAVCWVGGGRASPVLGTDRTSVSGISASRGHAAALSAAMLR"
     repeat_region   13571..13591
                     /gene="oriIS"
                     /product="ISCR3 oriIS"
     misc_feature    complement(14251..14652)
                     /gene="ΔtetA(G)-3'"
                     /product="Truncated Tetracycline efflux protein TetA,
                     class G, 3' fragment"
     mobile_element  14661..15540
                     /gene="IS6100"
                     /product="Insertion sequence: IS6100"
     repeat_region   14661..14674
                     /gene="IRL_IS6100"
                     /product="IS6100 inverted repeat left"
     CDS             14722..15486
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6100 transposase"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     repeat_region   15527..15540
                     /gene="IRR_IS6100"
                     /product="IS6100 inverted repeat right"
BASE COUNT     3146 a   4563 c   4626 g   3205 t
ORIGIN
        1 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
       61 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
      121 ctttccgcaa ccggctgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
      181 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
      241 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
      301 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
      361 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
      421 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
      481 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
      541 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
      601 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
      661 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
      721 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
      781 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
      841 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
      901 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
      961 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     1021 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     1081 ggatgaaggc acgaacccag tggacataag cctgttcggt tggtaagctg taatgcaagt
     1141 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1201 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat
     1261 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1321 ttacgcagca gggcagtcgc cctaaaacaa agttaggcat cacaaagtac agcatcgtga
     1381 ccaacagcaa cgattccgtc acactgcgcc tcatgactga gcatgacctt gcgatgctct
     1441 atgagtggct aaatcgatct catatcgtcg agtggtgggg cggagaagaa gcacgcccga
     1501 cacttgctga cgtacaggaa cagtacttgc caagcgtttt agcgcaagag tccgtcactc
     1561 catacattgc aatgctgaat ggagagccga ttgggtatgc ccagtcgtac gttgctcttg
     1621 gaagcgggga cggatggtgg gaagaagaaa ccgatccagg agtacgcgga atagaccagt
     1681 cactggcgaa tgcatcacaa ctgggcaaag gcttgggaac caagctggtt cgagctctgg
     1741 ttgagttgct gttcaatgat cccgaggtca ccaagatcca aacggacccg tcgccgagca
     1801 acttgcgagc gatccgatgc tacgagaaag cggggtttga gaagcaaggt accgtaacca
     1861 ccccagatgg tccagccgtg tacatggttc aaacacgcca ggcattcgag cgaacacgca
     1921 gtgatgccta acccttccat cgagggggac gtccaagggc tggcgccctt ggccgcccct
     1981 catgtcaaac gttagccata ttatggagcc tcatgctttt atataaaatg tgtgacaatc
     2041 aaaattatgg ggttacttac atgaagtttt tattggcatt ttcgctttta ataccatccg
     2101 tggtttttgc aagtagttca aagtttcagc aagttgaaca agacgttaag gcaattgaag
     2161 tttctctttc tgctcgtata ggtgtttccg ttcttgatac tcaaaatgga gaatattggg
     2221 attacaatgg caatcagcgc ttcccgttaa caagtacttt taaaacaata gcttgcgcta
     2281 aattactata tgatgctgag caaggaaaag ttaatcctaa tagtacagtc gagattaaga
     2341 aagcagatct tgtgacctat tcccctgtaa tagaaaagca agtagggcag gcaatcacac
     2401 tcgatgatgc gtgcttcgca actatgacta caagtgataa tactgcggca aatatcatcc
     2461 taagtgctgt aggtggcccc aaaggcgtta ctgatttttt aagacaaatt ggggacaaag
     2521 agactcgtct agaccgtatt gagcctgatt taaatgaagg taagctcggt gatttgaggg
     2581 atacgacaac tcctaaggca atagccagta ctttgaataa atttttattt ggttccgcgc
     2641 tatctgaaat gaaccagaaa aaattagagt cttggatggt gaacaatcaa gtcactggta
     2701 atttactacg ttcagtattg ccggcgggat ggaacattgc ggatcgctca ggtgctggcg
     2761 gatttggtgc tcggagtatt acagcagttg tgtggagtga gcatcaagcc ccaattattg
     2821 tgagcatcta tctagctcaa acacaggctt caatggcaga gcgaaatgat gcgattgtta
     2881 aaattggtca ttcaattttt gacgtttata catcacagtc gcgctgataa ggctaacaag
     2941 gccatcaagt tgacggcttt tccgtcgctt gttttgtggt ttaacgctac gctaccacaa
     3001 aacaatcaac tccaaagccg caacttatgg cggcgttaga catcatgagg gtagcggtga
     3061 ccatcgaaat ttcgaaccaa ctatcagagg tgctaagcgt cattgagcgc catctggaat
     3121 caacgttgct ggccgtgcat ttgtacggct ccgcagtgga tggcggcctg aagccataca
     3181 gcgatattga tttgttggtt actgtggccg taaagcttga tgaaacgacg cggcgagcat
     3241 tgctcaatga ccttatggag gcttcggctt tccctggcga gagcgagacg ctccgcgcta
     3301 tagaagtcac ccttgtcgtg catgacgaca tcatcccgtg gcgttatccg gctaagcgcg
     3361 agctgcaatt tggagaatgg cagcgcaatg acattcttgc gggtatcttc gagccagcca
     3421 tgatcgacat tgatctagct atcctgctta caaaagcaag agaacatagc gttgccttgg
     3481 taggtccggc agcggaggaa ttctttgacc cggttcctga acaggatcta ttcgaggcgc
     3541 tgagggaaac cttgaagcta tggaactcgc agcccgactg ggccggcgat gagcgaaatg
     3601 tagtgcttac gttgtcccgc atttggtaca gcgcaataac cggcaaaatc gcgccgaagg
     3661 atgtcgctgc cgactgggca ataaaacgcc tacctgccca gtatcagccc gtcttacttg
     3721 aagctaagca agcttatctg ggacaaaaag aagatcactt ggcctcacgc gcagatcact
     3781 tggaagaatt tattcgcttt gtgaaaggcg agatcatcaa gtcagttggt aaatgatgtc
     3841 taacaattcg ttcaagccga ccgcgctacg cgcggcggct taactccggc gttagatgca
     3901 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa
     3961 gcgtgcataa taagccctac acaaattggg agatatatca tgaaaggctg gctttttctt
     4021 gttatcgcaa tagttggcga agtaatcgca acatccgcat taaaatctag cgagggcttt
     4081 actaagcttg ccccttccgc cgttgtcata atcggttatg gcatcgcatt ttattttctt
     4141 tctctggttc tgaaatccat ccctgtcggt gttgcttatg cagtctggtc gggactcggc
     4201 gtcgtcataa ttacagccat tgcctggttg cttcatgggc aaaagcttga tgcgtggggc
     4261 tttgtaggta tggggctcat aattgctgcc tttttgctcg cccgatcccc atcgtggaag
     4321 tcgctgcgga ggccgacgcc atggtgacgg tgttcggcat tctgaatctc accgaggact
     4381 ccttcttcga tgagagccgg cggctagacc ccgccggcgc tgtcaccgcg gcgatcgaaa
     4441 tgctgcgagt cggatcagac gtcgtggatg tcggaccggc cgccagccat ccggacgcga
     4501 ggcctgtatc gccggccgat gagatcagac gtattgcgcc gctcttagac gccctgtccg
     4561 atcagatgca ccgtgtttca atcgacagct tccaaccgga aacccagcgc tatgcgctca
     4621 agcgcggcgt gggctacctg aacgatatcc aaggatttcc tgaccctgcg ctctatcccg
     4681 atattgctga ggcggactgc aggctggtgg ttatgcactc agcgcagcgg gatggcatcg
     4741 ccacccgcac cggtcacctt cgacccgaag acgcgctcga cgagattgtg cggttcttcg
     4801 aggcgcgggt ttccgccttg cgacggagcg gggtcgctgc cgaccggctc atcctcgatc
     4861 cggggatgga ccggcgattg gtccatggcg aaacggccga ccttgcgccg accggcttcg
     4921 ggcaacaggt ccgcgaagcc atggaccagc gccgcgagca tcatatcgaa cagcgcgacg
     4981 ccacgcgcaa cagggacggc cgaatcttct accggcgcaa tcttctcgcc accctgcgcg
     5041 agcgggaagt tgcgcgcgcc ggtgcggaga tggccgaggg caaggcgctg ccgttccgcg
     5101 ccgccaagga tggtgagagt gtcagcggca agttcaccgg gactgtccag ctaacgagcg
     5161 gcaagttcgc catcgtggaa aagagccacg agttcaccct tgtcccgtgg cggccgatca
     5221 tcgaccgcca gctcggccgc gaggtcgcgg gtatcatgca gggcggttcg gtgtcgtggc
     5281 agttagggcg gcagcggggg ttggggctat aggagccaac gataccgcat tgcaacgcaa
     5341 caactaattc gataagagct gctattcaat ttcgtaatcg tggagccatc agcatgggaa
     5401 attccaagtc agcagacaag taagccgcaa catcagaatt gttgttgcgg cgctctgtaa
     5461 gaccaatccc atctgattgc tgacgagcag acgctgcccg gtatccttaa tcgagcggtt
     5521 gattcgtcat gaccaccaca cgccccgcgt gggcctatac gctgccggca gccttgctgc
     5581 ttatggctcc cttcgacatc ctcgcctcgc tggcgatgga tatttatctt ccagtcgttc
     5641 cggcgatgcc gggcgtcctg aacacgactc catccataat ccaactcacg ttgagcctct
     5701 acatggtgat gctcggtgtg ggccaagtga tctttgggcc actctccgat cgcgtcgggc
     5761 gacggccgat cctgcttgta ggcgcaacgg ctttcgttgc tgcgtctctg ggagcggctt
     5821 gttcttcaac tgcattagcc tttgttgcgt ttcgtctggt tcaggctgtt ggagcatcgg
     5881 ccatgctggt ggccaccttc gcgaccgtgc gcgacgtata tgccaatcgt cccgaaggtg
     5941 ccgtcatcta cggccttttc agttcgatgc tggcgttcgt gcctgcgctc ggccctatag
     6001 ccggtgcgct gatcggcgag ttttggggat ggcaggcgat cttcatcaca ctggctgcac
     6061 tggcttcgct cgcactctta aacgccagtt tcaggtggca tgaaacccga ccgttggatc
     6121 aggccagaac gcaacgatct gttttgccga tcttcgcgag tccggccttt tgggtttaca
     6181 cggtcggatt tagtgccggc atgggcacat tcttcgtttt cttctcgaca gccccccgtg
     6241 ttctcatagg ccaagccggc tattccgaga tcggatttag cttggccttc gcgactgtcg
     6301 cgctggtcat ggtcacgaca acccgcttcg caaagtcctt cgttgccaaa tggggtatcg
     6361 cgggatgcgt agcgcgcggg atggcgttgc tcgtttccgg cgcgatcctg ttggggatcg
     6421 gccaactttt cggatcgccg tcatttttca gcttcatcct gccgatgtgg gttgtcgcgg
     6481 tcggcattgt cttcacggtg tccgttaccg ccaacggcgc acttgcgcag ttcgacgaca
     6541 tcgctggatc agcggttgcg ttctacttct gcatccaaag cctgatagtc agtatcgtcg
     6601 ggacattggc ggtgacgctg ttaaacggcg atacagcgtg gcccgtgatt tgttacgcca
     6661 cggcaatggc agtgctggtg tcgttggggc tggcgctcct tcgatcccgt gatgctgcca
     6721 ccgagaagtc gccagtcgtc tagccgacga ctggaagcaa gcccgctccg atgcggcgca
     6781 ataatcttcg aaacctcgtg aatggcggta tcctgtctgg caagataccg ctcatttccc
     6841 ttgtcccgtg gcggccggtc atcgaccgcc agctcggccg tgaggtcatg ggcatcgtgc
     6901 aaagcggatc ggtgtcgtgg cagttggggc ggcaaagggg cataagcctc taatctgttg
     6961 tagatgaacg cagccgctcg aaaccagcga tgaggctgtc gagtccgaag ttgaacgcag
     7021 catccatgcc gtctgtttcc aactcgtgaa acagatcgtg caggaaggac gacggtgctt
     7081 gctcggacac atctggcctg tccggaactc tctcatcggc atcagatgcc tgctgctcga
     7141 gaacggaacc gaccacatag tgactgaccg cccggagcgc ccaaacggcg cgcttcggac
     7201 aaaagccctc cgcgcagaga aagcgtattt gcgtctcggc ggtgccaaaa ttcggttctg
     7261 tcggtcgagt gccggcatgg atacgcgcgc cgtcccgata agagagcaac gccgttctga
     7321 agctcagggc attctctttc aggaacaccc gccagtcctc attctcttcg ggtagcgagc
     7381 gggtatggcg ttccgccagc atcgcctcgg cgagcgcatc aagcagcgct cgcttgttct
     7441 ggaaatgcca gtaaagcgca ggctgctgaa ccttgaggcg ttcagcgagc ttccgcgtcg
     7501 tcaggctgtc catgccaacc tcgttcaaca gctctagcgc cgccgcgatc acggtgccct
     7561 tgtccagttt ggtcattcac gttccttcgc cagtgcttga caatttatca ccgataagtt
     7621 atatgtccat ctccttatcg ttgataaagt cgctccattg agcggcgctg gagtttcagg
     7681 tgcgcagctc tgccatcatt gccctgctga tcgtgggtct tgacgccatg ggtctcggcc
     7741 tcatcatgcc cgtccttccg acgcttctgc gtgagcttgt gccagcagag caggtcgctg
     7801 gacactatgg tgccttgctg tcgctctatg cattgatgca ggtcgtcttc gcgcccatgc
     7861 ttggacagct ttcggattct tacggtcggc gtccggtact tctggcttct cttgcaggag
     7921 ccgcagtcga ttacacgatt atggcatcag cgccggtctt atgggtgctc tatatcggcc
     7981 gactcgtgtc cggcgtcacg ggcgcaaccg gagctgtagc agcctcaacc attgccgatt
     8041 cgacggggga aggttctcgc gcacgctggt tcggctacat gggggcctgt tatggggcgg
     8101 gcatgattgc cgggccagca cttggtggca tgctcggtgg tatctctgct catgccccgt
     8161 ttatcgccgc cgcccttctc aacgggttcg cgttcctgct tgcctgcatt ttcctcaagg
     8221 agactcatca cagccatggc gggaccggaa agccggttcg catcaaacca ttcgttctgt
     8281 tacggctgga tgatgcattg cgcgggctag gtgcgctttt cgcagttttc ttcattattc
     8341 aactgatcgg ccaagtgcct gcagccctat gggtcatata tggcgaggac cgttttcagt
     8401 ggaacaccgc gaccgttggt ttgtcgctcg cggcgtttgg ggcaacacat gcgggctctg
     8461 ttgcaaagat tggcggcagt cagaggtagg ctgtcgctct gcgccgatca ggcggctgct
     8521 gcgaaatggt ggttgagcat gcccatggcc tccgtcagcg ccgagggccc aatgccaaaa
     8581 gctctctcca caaggcgcac ctcgcccctg atgccgggct gcaggcacca ggggcgagcc
     8641 tgtcctttgc gcagggctcg catgacttcg aatcccttga tcgtggcata ggccgtgggg
     8701 atcgatttga aaccgcgcac cggcttgatc agtatcttga gctttccgtg atcggcctcg
     8761 atcacgttat tgagatactt cacctgccgg tgggccgtct cccggtccag ctttccttcg
     8821 cgcttcaatt cggtgatcgc tgcaccatag ctcggcgctt tgtcggtatt gagcgtggca
     8881 ggcttttccc agtgcttcag gcctcgcagg gccttgccca ggaaccgctt cgctgccttg
     8941 gcgctgcggg tcggcgacag gtagaaatcg atcgtgtcgc cccgcttgtc gactgcccgg
     9001 tacaggtagg tccacttgcc ccgcaccttg acgtaggttt catccaggcg ccagctcgga
     9061 tcaaagccac gccgccagaa ccagcgcagc cgcttctcca tctccggggc gtagcactgg
     9121 acccagcgat agatcgtcgt atggtcgacc gaaatgccgc gttccgccag catttcctca
     9181 aggtcgcgat agctgatcgg atagcgacaa taccagcgca ccgcccacag gatcacatca
     9241 ccctggaaat ggcgccactt gaaatccgtc atcgttccgt ccgtccaatc tccgccaagc
     9301 atgctcaagc ttcacgattt ttgcaacaga gcccctcgat gcccgaacgg acgttagatt
     9361 tcgagttcta ggcgttctgc gatgaaggtt ggatcccagc cgggattgaa agtgtcgacg
     9421 tgggtgaatc cgagccgctc gtataggcca cgcaggttcg ggtggcagtc gagccgcagc
     9481 ttggcgcacc cctgcgttcg cgcggcatgg cggcaagcct cgatcagcgc ggagctgaca
     9541 ccccggcccg catgtgtccg tcgcaccgcg agcttgtgca gatatgcggc ctcccccttg
     9601 agggcgtcgg gccagaactc gggatcctcg gccgacaagg tgcaacagcc gacgatgccg
     9661 tcgctgcaac tcgcgactag gagctcggat ctcaggacga aggtctccgc gaatgtccgg
     9721 tcgatccgcg cgacgtccca ggcgggcgtt cccttggcgg acatccacgc cgcagcgtcg
     9781 tgcatcagcc gcacaacctc gtcgatatca cccgagcagg cgacccgaac gttcggaggc
     9841 tcctcgctgt ccattcgctc ccctggcgcg gtatgaaccg ccgcctcata gtgcagtttg
     9901 atcctgacga gcccagcatg tctgcgccca ccttcgcgga acctgaccag ggtccgctag
     9961 cgggcggccg gaaggtgaat gctaggcatg atctaaccct cggtctctgg cgtcgcgact
    10021 gcgaaatttc gcgagggttt ccgagaaggt gattgcgctt cgcagatctc caggcgcgtg
    10081 ggtgcggacg tagtcagcgc cattgccgat cgcgtgaagt tccgccgcaa ggctcgctgg
    10141 acccagatcc tttacaggaa ggccaacggt ggcgcccaag aaggatttcc gcgacaccga
    10201 gaccaatagc ggaagcccca acgccgactt cagcttttga aggttcgaca gcacgtgcag
    10261 cgatgtttcc ggtgcggggc tcaagaaaaa tcccatcccc ggatcgagga tgagccggtc
    10321 ggcagcgacc ccgctccgtc gcaaggcgga aacccgcgcc tcgaagaacc gcacaatctc
    10381 gtcgagcgcg tcttcgggtc gaaggtgacc ggtgcgggtg gcgatgccat cccgctgcgc
    10441 tgagtgcata accaccagcc tgcagtccgc ctcagcaata tcgggataga gcgcagggtc
    10501 aggaaatcct tggatatcgt tcaggtagcc cacgccgcgc ttgagcgcat agcgctgggt
    10561 ttccggttgg aagctgtcga ttgaaacacg gtgcatctga tcggacaggg cgtctaagag
    10621 cggcgcaata cgtctgatct catcggccgg cgatacaggc ctcgcgtccg gatggctggc
    10681 ggccggtccg acatccacga cgtctgatcc gactcgcagc atttcgatcg ccgcggtgac
    10741 agcgccggcg gggtctagcc gccggctctc atcgaagaag gagtcctcgg tgagattcag
    10801 aatgccgaac accgtcaccc tgctgcgtaa catcgttgct gctccataac atcaaacatc
    10861 gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac
    10921 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
    10981 ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg
    11041 cttatgtcca ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct
    11101 tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg
    11161 tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag gtgctgtgca
    11221 cggatctgcc ctggcagggc atcaacgaag accagaacct gggcatcgcc atcacccgcc
    11281 gtgcgctgga agccccgctg cgcgccatcg tggccaacgc cggtgaagaa ccgagcgtga
    11341 tcgtggccaa cgtcaaggcc ggcgaaggca gctacggcta caacgccgcc accggcgagt
    11401 tcggcgacat gatcgccatg ggcatcctgg acccgaccaa ggtgacccgc tcggccctgc
    11461 agcacgccgc ttccgtcgcc ggccttgcga tcacgaccga agtggtcgtg gccgaagtgc
    11521 cgaagaagga agagccggcc atgccgggtg ctggcggtat gggcggcatg ggcggcatgg
    11581 atttctgatc cggttggccc ggtcgtcagg gaaccggacc gcgccagcgc ggtccgatcc
    11641 cggcaacgac ccgacatcaa ggccccaagg acggggccgg agcccggcag cgatgccggg
    11701 ctttttgttg tgcccgcgcc gcggcaatgt ctgacgcgaa gatcagaacg caccgatacg
    11761 aacgtgcgaa cacaggcgca acactgagca gccgtccccg caccggagcg ctgcgtgccg
    11821 cgcctcgcca catcccggcg gcaagccgcg ggatgcgcgc cactgccgtc cgcccacacc
    11881 ggttcgcggt acgcgcgcca cgcgcccgag cgcacgctgc tgtacgcgtt ggtagaggcg
    11941 cactacccgg acttcattgc acggatcgaa gcggagggcc gctcgctgcc cgggtatgtc
    12001 cgcgaggcgt tcgatgccta cctgcgttgc ggcgtactcg agcacggctt cctgcgggtg
    12061 gtgtgcgagc actgccgtgc agagaggctg gtggccttct cctgcaagaa gcgcgggttc
    12121 tgcccgagtt gcggcgcgcg acgcatggcc gagagtgcgc ggcacctggt cgaggaggtg
    12181 ttcggcccgc ggcctgtgcg gcaatgggtg ctgagctttc cgtacccctt gcgtttcctg
    12241 ttcgccagca agccagaagc cattggcccg gtgctgggca tcgtgcagcg cgtgatcgcc
    12301 ggctggttgg ccgatcaagc cggcatcgac cgcgccagcg cccagtgcgg cgcggtgacg
    12361 ctgatccagc gtttcggcag cgcgctgaac ctgaacatcc acttccacat gctgtggctc
    12421 gacggcgtgt acgtggaagc caccgagctg ccgcggcgcg aactgcgcct gcaccgcgcc
    12481 cgtgcgccca ccaccgcgca gttgacccag ctggcagcta ccatcgcgca ccgggtgtgt
    12541 cggcacctga cgcgcaaagg ctggctcgaa ggggagggcg aatcggcctt cctggcagac
    12601 agcgctgcag gcgacgacag catggatggg ctgcggatga gttcgatcac ctaccgcatc
    12661 gccaccggcc gcgacgctgg ctgcaaggtc gtcacgctgc aaacgctgcc cggtgacgcc
    12721 ggttcgctgg agggcgaagc cggcaaggtc ggcggcttct cactgcatgc cggcgtggcg
    12781 gccgaagcac acgaaagcca caagctggaa aagctgtgcc gctacatcac gcgcccggcg
    12841 atcagcgaga agcggctgtc gatagcgctc cagggcaggg tgcgttacca gctcaagacc
    12901 ccgtggcgca atggcaccac gcatgtggaa tgggatccgg tggatttcat cgccaagctg
    12961 gcggcgctgg tcccgccacc tcgcgcgcat ctcacccgct tccacggcgt attcgccccg
    13021 aatgcaaacc tgcgtgcgca gctgacgccc tcggggcgcg gcaagcggcc tgcgggcgat
    13081 gcggcgccag tggacgtcag cgcccacgac gcgccgcgca gccccgagga gaagcgccgt
    13141 gcgatgagct gggcgcaacg gctcaagcgg gtcttttcca tcgacgtcac cgcctgcgtc
    13201 cactgcggtg gcaccgtgcg gatcgtcgcc agcatcgagg aacccaccgc catccgcgcc
    13261 atcctcgccc acttcgagaa gcacggcgcg cgggaagaag cgcactacag gcccgcagcg
    13321 cgcgcgccgc cagtgcaagc cgcgtgacga tctgccggct gcacagccga cggcgaaacc
    13381 ggaatccgag ccgatgcggc cacgatccgc agggcggcgc tcggcccgct gtcgggaatc
    13441 agcgaagcat ggctgctgac aacgccgctg cgtggccccg cgatgccgaa atcccactca
    13501 cagacgtccg atccgtgccc aaaacggggc ttgcgcgacc gccgcctacc cagcagactg
    13561 cccgaaaagg gcgtttgaac ttcctatacg caaggaacgt ctcgcctgcc aaatcgggcc
    13621 atgtgacggc tgaccgcttg gcgagcggat gccgttccgg tagcaccgcc aagagcggtt
    13681 cggtccatgt gcgacgggaa tggcagtcgg gtggttgggg cgtgcccgcg acgaacgcca
    13741 cgtccaacct gccggcgcga agctgcacca ccgcttcacg ggccgggcct tcggcgatct
    13801 cgacttcaac atcggggtaa tccttgcggt attggccgat cagctttgcg aggaagctat
    13861 gcggaatcag ggcatggata ccgatacgaa gccggccgct ttctccggct gccgccatgc
    13921 cggcggtttt caccgcatgg tcgagttggt caatacctac ggctatccgc tcgacgaaat
    13981 ggcgtccggc ctcggtcagc cgaacgccgc gcgcatgacg ctcgaacaag aggatgccga
    14041 ggtcttcttc cagtgccttc acgcgggcgc tgacgctgga ctgtgcaacg ccgagcgcgt
    14101 tggcggcgtg acggaagttg agatattcgg cgacagcgag agtgtgaact aaggtcatca
    14161 ttggcactcg ccccgaaagg agatgattgc tcaacagatt taatccgtca tttctcctaa
    14221 gtctacgcat gccaaatcgc catgactaaa tcacaatgaa gttgcgaatg gtctgcgtag
    14281 tattggcaga catattaaat agaggatcgc gccgacaatc caaacccaac cgttccatgc
    14341 cccggcggtg gcagaataga gtgctgtgaa gccaagcggt cctgcgatag agcttagatt
    14401 ggtgaggctc gttagcgttc cttgcaaagc cccttgcttg ttactgctga cattgtttga
    14461 gagcattgcc tgcaaggccg gcatgccaac acccccggcg gcaagcagca acagaatcgg
    14521 gaacaccatc catccctgcg tggcaaaagc cagaagaacg aagccagtcg catccgcagc
    14581 catgccaaac agcagcgtgc gccgctctcc aagccggctt gaaagcgggc cggtaacaaa
    14641 cgcttggaag atcgcatgtg ggctctgttg caaaaatcgt gaagcttgag catgcttggc
    14701 ggagattgga cggacggaac gatgacggat ttcaagtggc gccatttcca gggtgatgtg
    14761 atcctgtggg cggtgcgctg gtattgtcgc tatccgatca gctatcgcga ccttgaggaa
    14821 atgctggcgg aacgcggcat ttcggtcgac catacgacga tctatcgctg ggtccagtgc
    14881 tacgccccgg agatggagaa gcggctgcgc tggttctggc ggcgtggctt tgatccgagc
    14941 tggcgcctgg atgaaaccta cgtcaaggtg cggggcaagt ggacctacct gtaccgggca
    15001 gtcgacaagc ggggcgacac gatcgatttc tacctgtcgc cgacccgcag cgccaaggca
    15061 gcgaagcggt tcctgggcaa ggccctgcga ggcctgaagc actgggaaaa gcctgccacg
    15121 ctcaataccg acaaagcgcc gagctatggt gcagcgatca ccgaattgaa gcgcgaagga
    15181 aagctggacc gggagacggc ccaccggcag gtgaagtatc tcaataacgt gatcgaggcc
    15241 gatcacggaa agctcaagat actgatcaag ccggtgcgcg gtttcaaatc gatccccacg
    15301 gcctatgcca cgatcaaggg attcgaagtc atgcgagccc tgcgcaaagg acaggctcgc
    15361 ccctggtgcc tgcagcccgg catcaggggc gaggtgcgcc ttgtggagag agcttttggc
    15421 attgggccct cggcgctgac ggaggccatg ggcatgctca accaccattt cgcagcagcc
    15481 gcctgatcgg cgcagagcga cagcctacct ctgactgccg ccaatctttg caacagagcc
//
