LOCUS       In173                   7105 bp    DNA     linear       29-SEP-2020
DEFINITION  Pseudomonas aeruginosa.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Unclassified.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of In173
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7105)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
     repeat_region   1..25
                     /gene="IRi_In173"
     CDS             complement(203..1216)
                     /gene="intI1"
                     /codon_start=1
                     /product="Integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     misc_recomb     1297..1359
                     /gene="attI1"
     CDS             1396..2259
                     /gene="blaGES-1"
                     /codon_start=1
                     /product="Beta-lactamase GES-1"
                     /translation="MRFIHALLLAGIAHSAYASEKLTFKTDLEKLEREKAAQIGVAIV
                     DPQGEIVAGHRMAQRFAMCSTFKFPLAALVFERIDSGTERGDRKLSYGPDMIVEWSPA
                     TERFLASGHMTVLEAAQAAVQLSDNGATNLLLREIGGPAAMTQYFRKIGDSVSRLDRK
                     EPEMGDNTPGDLRDTTTPIAMARTVAKVLYGGALTSTSTHTIERWLIGNQTGDATLRA
                     GFPKDWVVGEKTGTCANGGRNDIGFFKAQERDYAVAVYTTAPKLSAVERDELVASVGQ
                     VITQLILSTDK"
     misc_recomb     2269..2378
                     /gene="attC_GES-1"
     CDS             complement(2475..3113)
                     /gene="tniR"
                     /codon_start=1
                     /product="Serine resolvase TniR"
                     /translation="-
                     TGEKVLIGYMRVSKADGSQSTNLQRDALIAAGVSLAHLYEDLASGRRDDRPGLAACLK
                     ALREGDTLIVWKLDRLGRDLRHLINTVHDLTARSVGLKVLTGHGAAVDTTTAAGKLVF
                     GIFAALAEFERELISERTVAGLISARARGRKGGRPFKMTAAKLRLAMASMGQPETKVG
                     DLCEELGITRQTLYRHVSPKGELRPDGVKLLSLGSAA"
     misc_recomb     3109..3136
                     /gene="res"
     CDS             complement(3160..4377)
                     /gene="tniQ"
                     /codon_start=1
                     /product="Transposition auxiliary protein TniQ"
                     /translation="-
                     KPAPRWPLHPAPKEGEALSSWLNRVALCYHMEEPDLLEHDLGHGQVDDLDTAPPLSLL
                     ALLSQRSGIELDRLRCMSFAGWVPWLLDSLDDQIPDALETYAFQLSVLLPRLRRKTRS
                     ITSWRAWLPSQPINRACPLCLSDPENQAVLLAWKLPLMLSCPLHGCWLESYWGVPGRF
                     LGWENADAEPRTASDAIAAMDQRTWQALTTGHVELPRRRIHAGLWFRLLRTLLDELNT
                     PLSACGTCAGYPRQVWEGCGHPLRAGQSLWRPYETLNPIVRLQMLEAAATAISLIEVR
                     DISPPGEQAKLFWSEPQTGFTSGLPTKAPKPEPINHWQRAVQAIDEAIIEARHNPETA
                     RSLFALASYGRRDPASLERLRATFVKEGIPPEFLSHYLPDAPFACLKQNDGLSDKF"
     CDS             complement(4374..5282)
                     /gene="tniB"
                     /codon_start=1
                     /product="ATP-binding protein TniB"
                     /translation="-
                     DEYPIIDLSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVEALNRLEALYAWPNKQ
                     RMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQMPSEPSVIRFYVALL
                     AAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAGNSVNRREFLNLLRFL
                     GNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDDCCSLLASFAASLPLR
                     RPSPIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEAINHRTLSMAVYTGPS
                     ERRRQFERELM"
     CDS             complement(5285..6964)
                     /gene="tniA"
                     /codon_start=1
                     /product="DD(35)E transposase TniA"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVVTLEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLV
                     DFLPILRRTLTRTGFVIDHIHYYADALKPWIARRERWPSFLIRRDPRDISRIWVLEPE
                     GQHYLEIPYRTLSHPAVTLWEQRQALAKLRQQGREQVDESALFRMIGQMREIVTSAQK
                     ATRKARRDADRRQHLKTSARPDKPVPPDTDIADPQADNLPPAKPFDQIEEW"
     repeat_region   7081..7105
                     /gene="IRt_In173"
ORIGIN      
        1 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
       61 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
      121 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
      181 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
      241 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
      301 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
      361 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
      421 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
      481 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
      541 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
      601 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
      661 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
      721 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
      781 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
      841 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
      901 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
      961 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     1021 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     1081 ggatgaaggc acgaacccag tggacatacg cctgttcggt tcgtaaactg taatgcaagt
     1141 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1201 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat
     1261 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1321 ttacgcagca gggcagtcgc cctaaaacaa agttagacgg gcgtacaaag ataatttcca
     1381 tctcaaggga tcaccatgcg cttcattcac gcactattac tggcagggat cgctcactct
     1441 gcatatgcgt cggaaaaatt aaccttcaag accgatcttg agaagctaga gcgcgaaaaa
     1501 gcagctcaga tcggtgttgc gatcgtcgat ccccaaggag agatcgtcgc gggccaccga
     1561 atggcgcagc gttttgcaat gtgctcaacg ttcaagtttc cgctagccgc gctggtcttt
     1621 gaaagaattg actcaggcac cgagcggggg gatcgaaaac tttcatatgg gccggacatg
     1681 atcgtcgaat ggtctcctgc cacggagcgg tttctagcat cgggacacat gacggttctc
     1741 gaggcagcgc aagctgcggt gcagcttagc gacaatgggg ctactaacct cttactgaga
     1801 gaaattggcg gacctgctgc aatgacgcag tattttcgta aaattggcga ctctgtgagt
     1861 cggctagacc ggaaagagcc ggagatgggc gacaacacac ctggcgacct cagagataca
     1921 actacgccta ttgctatggc acgtactgtg gctaaagtcc tctatggcgg cgcactgacg
     1981 tccacctcga cccacaccat tgagaggtgg ctgatcggaa accaaacggg agacgcgaca
     2041 ctacgagcgg gttttcctaa agattgggtt gttggagaga aaactggtac ctgcgccaac
     2101 gggggccgga acgacattgg tttttttaaa gcccaggaga gagattacgc tgtagcggtg
     2161 tatacaacgg ccccgaaact atcggccgta gaacgtgacg aattagttgc ctctgtcggt
     2221 caagttatta cacaactcat cctgagcacg gacaaatagt tgacgcccgt ctaacaattc
     2281 gttcaagccg acgttgcttc gtggcggcgc ttgcgtgcta cgctaagctt cgcacgccgc
     2341 ttgccactgc gcaccgcggc ttaactcagg cgttgaacga cagctttccc aaaagctcta
     2401 cggctgctct gggtcgacac cggtaatcgg atcgttgccg cactgaacag cgccccgttc
     2461 caggtcgcct ccatttatgc ggctgaaccg agggagagca gctttacgcc gtctggccgc
     2521 agttcgccct tgggcgacac gtgccggtag agcgtctgcc gggtaatccc gagttcttcg
     2581 cagagatcgc ccaccttggt ttccggttgc cccatgctgg ccatcgccag gcgtagcttg
     2641 gcggcggtca tcttgaaggg gcgcccccct ttcctgccgc gagcgcgcgc cgagataagt
     2701 ccagcgactg ttcgctcgga aatcaactca cgctcgaact cggccagcgc ggcaaaaata
     2761 ccgaacacaa gcttgccggc ggcagtcgtc gtgtcgaccg ccgcaccgtg accggtcagg
     2821 accttcaggc ccacgctacg cgcagttagg tcgtgcacgg tgttgatcag gtggcgcaga
     2881 tcacggccaa gccgatcgag cttccacacg atcagcgtgt ccccttcacg aagcgccttc
     2941 aggcaagcag ccaaccctgg gcgatcatcg cgcctgcccg aggccagatc ctcgtaaagg
     3001 tgcgcaaggc tcacaccagc ggcgatgagc gcatcgcgtt gcaaattggt ggactgggat
     3061 ccgtccgcct tcgatacccg catgtagccg atcagcacct tttcacccgt cacgtatacg
     3121 ttcgattatg tgacagtgtg agccggaaag ctctatccgt caaaatttgt cacttaaccc
     3181 gtcattttgt ttaagacatg caaagggtgc atcaggcagg taatgtgaca gaaattccgg
     3241 cgggatgcct tccttcacga aggtggcgcg caaccgttcc aaggaagcgg gatcgcgccg
     3301 accataggaa gccaacgcga acagcgagcg tgccgtctcg gggttgtgtc gcgcttcaat
     3361 gatggcctcg tcgatggcct ggactgcacg ctgccagtga ttgatgggct cgggcttcgg
     3421 cgctttcgtc ggcaggccac tggtgaaccc ggtttggggc tcggaccaga atagctttgc
     3481 ctgctcgcct ggcgggctga tgtccctcac ctcaatcaag ctgattgccg ttgccgccgc
     3541 ctccagcatc tgtaaccgta ctatcggatt cagggtttca tacggtcgcc acagactttg
     3601 cccagcacgc agcggatgcc cgcagccttc ccagacttgg cggggatacc ccgcgcaggt
     3661 tccgcacgcg gaaagcgggg tgttcagctc atcgagcagc gtgcgaagaa gtcgaaacca
     3721 caatccggcg tggatgcgtc ggcgcggcag ctccacgtga ccggttgtca gtgcctgcca
     3781 ggtacgctgg tccatcgccg caatcgcgtc gctggcggtg cgcggttcgg cgtcggcgtt
     3841 ctcccagccg agaaaccgcc ctggcacgcc ccaataggat tccagccagc agccatgcag
     3901 cgggcagctc agcatcaggg gcagcttcca cgcgagcagt acggcttggt tctccggatc
     3961 gctcaggcag agcggacagg cgcggtttat cggctggctg ggcagccagg cacgccagct
     4021 cgtgatggat cgcgtcttac ggcggagtct tggcagcaac accgagagct gaaacgcata
     4081 ggtttccaag gcgtctggaa tctggtcatc aaggctgtcc agtagccaag gcacccatcc
     4141 ggcgaaactc atacagcgca gccggtccag ctcgatgccg ctccgctggg aaagcaacgc
     4201 cagcagcgag agtggtggcg cggtgtccag gtcatcgacc tggccgtgac caagatcgtg
     4261 ctccagcagg tcgggctcct ccatgtgata gcaaagggcc acgcggttga gccatgagga
     4321 cagcgcctcg ccttctttcg gggcgggatg cagcggccag cgcggcgcag gcttcacatc
     4381 agttcccgct cgaattgccg ccgccgctcg ctgggtccgg tgtaaacggc catgctgagt
     4441 gtgcgatggt tgatcgcttc ctcgccgctc tccacggcga cgatggccgc cgccatcagc
     4501 aagtgcgcca gttcccctat ggtgccctcg ctgcgtgtga gcaggtagcg agccatgtcc
     4561 agcgtggcaa ttggggaagg ccggcgcagc gggagcgaag cggcgaagct ggccagcagt
     4621 gagcagcaat cgtcgttggc ctcccatacc ggcagcatca tcggctcgaa gcgattttcc
     4681 aactggtcat cggagcggat ggctaggtag gcgtcgcgcg tgcctacccc aaccaacggg
     4741 atgcgcagtt cgttgccgag gaagcgcagc aggttgagga attcccggcg gttgacgctg
     4801 ttgccggcca gcacgttgtg cagctcgtcg atcaccagca tgcgcacgcc gaccttgcgc
     4861 agcagtgcca gagccagttg ctccatttcc ggcaaccgtg ggcgtgggcg cagcggcgcg
     4921 cccatcgcgg cgagcagcgc gacgtagaag cggatcacgg acggctcgga cggcatctgc
     4981 acgaccaaca ccgggatgtg ctcctggtcg gcgtcggagc tggccgggtg ggtgcggcgg
     5041 aacttctcga cgatcatcga cttgccattg ttggtcgggc caaccagcag caggttgggc
     5101 atgcgttgct tgtttggcca cgcataaagg gcttccagcc ggttcagcgc ctcgactgcg
     5161 cgcggatagc cgatccagcg gtcggcgcga aggcgctgga tgcgctcgtc cgccggaaga
     5221 cgggccaagc cctgggccgc cggcagcagg tgggacaggt cgatgatggg atattcgtcc
     5281 acggctacca ctcctcaatc tggtcgaacg gtttggcggg tggcaagttg tctgcctgcg
     5341 ggtcggcaat atccgtatcc ggcggaacgg gcttgtccgg ccgagctgat gtcttgaggt
     5401 gctggcggcg atccgcgtca cgccgcgcct tgcgtgtggc cttctgcgcg ctggtcacaa
     5461 tctcacgcat ctggccgatc atgcggaaca gcgccgactc atccacctgt tcgcgccctt
     5521 gctgccgcag tttcgccagc gcctgccgtt gttcccagag ggtgacagcc ggatgcgaca
     5581 aggtacggta gggaatttcc aggtaatgct gtccctccgg ttccaggacc cagatacggc
     5641 tgatgtcgcg cggatcgcgc cggatcagaa aggacggcca gcgttcacgc cgcgcaatcc
     5701 acggcttgag cgcatcggcg tagtagtgga tgtggtcgat gacaaagccg gtgcgggtca
     5761 gcgtgcgccg gaggatcggc agaaaatcga ccaggaacga agtagcgcgt gtgacgacgg
     5821 ccggtacgcc gacacgcgcc acggcctcgg cccagcgcgc ggccggcggt tggagcaggc
     5881 cgttgtgcac cgaaccgtgg taggtgccga ccgccaatgt gagccagcgc tctagctcgc
     5941 gcagcgtcag ggcggccttg ttttcggaat cgtagtcgcc gcgctggtca gggttggaga
     6001 aggtcgttcc cggcagttcg tcgtgaatca tctgcatcgc cgtgccgatg atccgttcca
     6061 cgatgccgcc atagtgcggc tgtcccagcg ggcgatagtc cagccggatg ccatgctgct
     6121 cgcaaccccg gcgcagggcc tcgctcttga actcggccgc gttgtctagg tagagcagca
     6181 agggcttgcc gctcatctgc caatccattt ccacgttcag tccttccagc caagggcgct
     6241 tgtcgcaggc gacatgcacg aggcacaggc caaccgaaac ggcagacggc gcttccagcg
     6301 tgacgaccat gccgagcacg cagcgggtga acacgtcgat ggcgagggtc aggtacgggc
     6361 ggccaatagg ttgccggtcg cggtcatcga ccacgatcag gtcgatgacc gtatggtcta
     6421 tctgcacctg ctccagcggc gcggtcacgg caggaggctc gccgcccaca ccttgtaggt
     6481 cacgagcggc atcctggcct tcccgccggc ggatgacctt gcgcgggtca aggctagcga
     6541 tccgtaaggc cacggtattg cgcgccggca ctcgcagttt ttgagccttg cacacctgag
     6601 tgacttcgcg gtgaaaggcc gctaggctgc gcttctgctt ggtcaggaac cgcttttgca
     6661 gtagctcgtg gatgacgcgc tcgaccggtt ccggcaagcg ccccttacct ttacctccac
     6721 cggactggcc gggcaccaga tccgtcacga ggccgctgcc ttgccgggca cgccggatca
     6781 gaacgtatac ctggcgccga gacaagccca gcgcctgagc cgccatatcg gccgcttcgt
     6841 gcccgaccgt ctccgactgc gccaacggac tgatgatctc cgcacgacgg cgcgcacgct
     6901 cccaagcctc atcaggcaga gtggccacgc cttgttctgg aatccgtggg gtgtccgtcg
     6961 ccatgctcac ctcgctttgg tgcacacgag tattgagcat agtcgagatt ggtgcagatc
     7021 acttctgata ttgaactgtc aggagctggc tgcacaacag ccattacgcc caatcaactg
     7081 gtgcagtcgt cttctgaaaa tgaca
//
