LOCUS       In1222                 13505 bp    DNA     linear       29-SEP-2020
DEFINITION  Vibrio alginolyticus.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Vibrio alginolyticus
  ORGANISM  Vibrio alginolyticus
            Unclassified.
REFERENCE   1  (bases 1 to 13505)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of In1222
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13505)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..13505
                     /organism="Vibrio alginolyticus"
                     /mol_type="genomic DNA"
     repeat_region   1..25
                     /gene="IRi_In1222"
     CDS             complement(203..1216)
                     /gene="intI1"
                     /codon_start=1
                     /product="Integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     misc_recomb     1297..1359
                     /gene="attI1"
     CDS             1568..2224
                     /gene="qnrVC4"
                     /codon_start=1
                     /product="Fluoroquinolone resistance protein"
                     /translation="MDKTDQLYVQADFSHQDMSGQYFKNCKFFCCSFKRANLRDTQFV
                     DCSFIERGELEGCDFSYSDLRDASFKNCSLSMSYFKGANCFGIEFRECDLKGANFAQA
                     SFMNQVSNRMYFCSAYITGCNLSYANFERQCIEKCDLFENRWIGANLSGASFKESDLS
                     RGVFSEGCWSQCRLQGCDLSHSELYGLDPRKVDLTGVKICSWQQEQLLEQLGLIVVPD
                     "
     misc_recomb     2245..2372
                     /gene="attC_qnrVC4"
     CDS             2481..2813
                     /gene="qacF"
                     /codon_start=1
                     /product="Quaternary ammonium compound resistance protein"
                     /translation="-
                     KNWIFLAVSIFGEVIATSALKSSHGFTRLVPSVVVVAGYGLAFYFLSLALKSIPVGIA
                     YAVWAGLGIVLVAAIAWIFHGQKLDFWAFIGMGLIVSGVAVLNLLSKVSAH"
     misc_recomb     2824..2883
                     /gene="attC_qacF"
     CDS             2902..3456
                     /gene="aacA4'-17"
                     /codon_start=1
                     /product="Aminoglycoside acetyltransferase"
                     /translation="-
                     TNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLADVQEQYLPSVLAQES
                     VTPYIAMLNGEPIGYAQSYVALGSGDGWWEEETDPGVRGIDQSLANASQLGKGLGTKL
                     VRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFEKQGTVTTPDGPAVYMVQTRQ
                     AFERTRSDA"
     misc_recomb     3434..3499
                     /gene="attC_aacA4'-17"
     CDS             3671..4930
                     /gene="cmlA1g"
                     /codon_start=1
                     /product="Chloramphenicol resistance protein"
                     /translation="-
                     RSKNFSWRYSLAATVLLLSPFDLLASLGMDMYLPAVPFMPNALGTTASTIQLTLTTYL
                     VMIGAGQLLFGPLSDRLGRRPVLLGGGLAYVVASMGLALTSSAEVFLGLRILQACGAS
                     ACLVSTFATVRDIYAGREESNVIYGILGSMLAMVPAVGPLLGALVDMWLGWRAIFAFL
                     GLGMIAASAAAWRFWPETRVQRVAGLQWSQLLLPVKCLNFWLYTLCYAAGMGSFFVFF
                     SIAPGLMMGRQGVSQLGFSLLFATVAIAMVFTARFMGRVIPKWGSPSVLRMGMGCLIA
                     GAVLLAITEIWASQSVLGFIAPMWLVGIGVATAVSVSPNGALRGFDHVAGTVTAVYFC
                     LGGVLLGSIGTLIISLLPRNTAWPVVVYCLTLATVVLGLSCVSRVKGSRGQGEHDVVA
                     LQSAESTSNPNR"
     misc_recomb     4950..5008
                     /gene="attC_cmlA1g"
     CDS             5195..5995
                     /gene="blaOXA-10"
                     /codon_start=1
                     /product="Beta-lactamase OXA-10"
                     /translation="MKTFAAYVIIACLSSTALAGSITENTSWNKEFSAEAVNGVFVLC
                     KSSSKSCATNDLARASKEYLPASTFKIPNAIIGLETGVIKNEHQVFKWDGKPRAMKQW
                     ERDLTLRGAIQVSAVPVFQQIAREVGEVRMQKYLKKFSYGNQNISGGIDKFWLEGQLR
                     ISAVNQVEFLESLYLNKLSASKENQLIVKEALVTEAAPEYLVHSKTGFSGVGTESNPG
                     VAWWVGWVEKETEVYFFAFNMDIDNESKLPLRKSIPTKIMESEGIIGG"
     misc_recomb     5917..6008
                     /gene="attC_blaOXA-10"
     CDS             6012..6803
                     /gene="aadA1e"
                     /codon_start=1
                     /product="Aminoglycoside adenylyltransferase"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_recomb     6781..6824
                     /gene="attC_aadA1e"
     CDS             6829..7311
                     /gene="dfrA14b"
                     /codon_start=1
                     /product="Dihydrofolate reductase"
                     /translation="MRTLKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWL
                     LVGRKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGG
                     GEIYRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG
                     "
     misc_recomb     7306..7391
                     /gene="attC_dfrA14b"
     CDS             7532..7798
                     /gene="mobC"
                     /codon_start=1
                     /product="Mobilisation protein MobC"
                     /translation="MPVLTDEEVNTVRESCRQLGAIGRNLNQVARALNIEFRESDKLK
                     QEAIEKLAERIDQHLDHVSELFDKTWSRWHDCHFQKTTAPVDWA"
     repeat_region   complement(7757..7781)
                     /gene="IRt_In1222"
     repeat_region   7880..7893
                     /gene="IRL_IS6100"
     CDS             7941..8705
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS6100 transposase"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     repeat_region   8746..8759
                     /gene="IRR_IS6100"
     CDS             8871..9110
                     /gene="orf240"
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MLSRRARAATPTCPTHPPHRSPPPAMARLFAGRIGGVSSRPGTP
                     TPAQPYGSDSLSKNRPGALTQVSGWSPFVSRRPEW"
     CDS             complement(9198..9782)
                     /gene="mphR(A)"
                     /codon_start=1
                     /product="Negative regulator"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     misc_feature    complement(9782..11019)
                     /gene="mrx(A)"
                     /product="Positive regulator (pseudogene)"
     CDS             complement(11016..11921)
                     /gene="mph(A)"
                     /codon_start=1
                     /product="Macrolide phosphotransferase/macrolide
                     resistance"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     repeat_region   complement(11991..12004)
                     /gene="IRR_IS26"
     CDS             complement(12043..12747)
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS26 transposase"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     misc_feature    complement(12759..13400)
                     /gene="truncated tniA"
                     /product="Truncated DD(35)E transposase TniA"
     repeat_region   complement(12797..12810)
                     /gene="IRL_IS26"
     repeat_region   13481..13505
                     /gene="IRt_In1222"
ORIGIN      
        1 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
       61 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
      121 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
      181 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
      241 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
      301 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
      361 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
      421 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
      481 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
      541 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
      601 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
      661 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
      721 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
      781 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
      841 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
      901 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
      961 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     1021 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     1081 ggatgaaggc acgaacccag tggacataag cctgttcggt tggtaagctg taatgcaagt
     1141 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1201 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat
     1261 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1321 ttacgcagca gggcagtcgc cctaaaacaa agttatgtgt ttttcttgca agcgccatga
     1381 ttaatcaaaa cttttttcta gccatgcggc atgggtgaat taaaaaacag tgccttagag
     1441 cgtcaatttg gtaccttatt tttgcgattt taagtggttg taagttgctg tttttaaggt
     1501 gtttattttt cttgagaact ggttggtaaa tgttagtctt cgactcaaat agaaagaggg
     1561 ctagaaaatg gataaaacag accagttata tgtacaagca gacttttcac atcaagacat
     1621 gagtggtcag tattttaaaa attgcaaatt tttctgctgt tcctttaaac gagcgaacct
     1681 ccgcgataca caatttgtag attgttcttt cattgaacga ggtgaattag aggggtgtga
     1741 tttttcttac tcggatctta gagatgcatc ttttaaaaac tgcagtcttt caatgtcgta
     1801 tttcaaaggt gcaaattgtt ttggtatcga gttcagagaa tgcgatttaa agggtgccaa
     1861 ttttgctcaa gctagcttca tgaatcaggt atcgaacaga atgtattttt gttcagctta
     1921 tataacaggt tgtaatcttt catacgccaa ctttgaaagg cagtgtatcg aaaagtgtga
     1981 tttgtttgag aatagatgga ttggcgcaaa tctgagtggt gcatcattta aagagtctga
     2041 tttaagtcgg ggagtatttt ctgaagggtg ttggagccag tgtaggttgc aaggttgtga
     2101 tttgagccac tcggagttgt atggtttaga ccctcggaaa gttgacctta caggtgtaaa
     2161 aatctgttcg tggcagcaag aacaactttt agagcaatta ggtttaatag tagttcctga
     2221 ctaaatttga tggtttgaac acacacataa caaacgcctc aagagggact gtcaacgcgt
     2281 ggcgtttcca gtcccaatga gctgtggtgg ttacggctgt tgtgtttggg tttagtgtca
     2341 tgcgttgcca gccccttagg cgggcgttag atgccagatt tggcgttgcg tatgctcaca
     2401 gaaaatcgat agccgcaaga ctgtttttgg caactcatag ccactacaat ttctccttca
     2461 taccgtagag gagattgcgc gtgaagaact ggatatttct ggctgtttca atctttggcg
     2521 aggtcatcgc aacttccgca ctgaagtcta gccatggatt cactaggtta gttccttccg
     2581 ttgtagttgt ggctggctac gggcttgcgt tctatttctt gtctctcgcg ctcaagtcca
     2641 ttccggtcgg tattgcttac gctgtatggg ctgggcttgg catcgtgctt gtggcagcta
     2701 ttgcttggat tttccatggc caaaaactag acttctgggc gttcattggc atgggactta
     2761 tcgtcagtgg cgtcgccgtt ctaaacctgc tatccaaggt cagcgcacat tgaccgggtt
     2821 ggcatctaac aattcattca agccgacgcc gcttcgcggc gcggcttaat tcaggcgtta
     2881 ggcatcacaa agtacagcat cgtgaccaac agcaacgatt ccgtcacact gcgcctcatg
     2941 actgagcatg accttgcgat gctctatgag tggctaaatc gatctcatat cgtcgagtgg
     3001 tggggcggag aagaagcacg cccgacactt gctgacgtac aggaacagta cttgccaagc
     3061 gttttagcgc aagagtccgt cactccatac attgcaatgc tgaatggaga gccgattggg
     3121 tatgcccagt cgtacgttgc tcttggaagc ggggacggat ggtgggaaga agaaaccgat
     3181 ccaggagtac gcggaataga ccagtcactg gcgaatgcat cacaactggg caaaggcttg
     3241 ggaaccaagc tggttcgagc tctggttgag ttgctgttca atgatcccga ggtcaccaag
     3301 atccaaacgg acccgtcgcc gagcaacttg cgagcgatcc gatgctacga gaaagcgggg
     3361 tttgagaagc aaggtaccgt aaccacccca gatggtccag ccgtgtacat ggttcaaaca
     3421 cgccaggcat tcgagcgaac acgcagtgat gcctaagtca aacgttgggc gcacaataag
     3481 gctcctcgca gagttgcttg aaagttgtta cgattcaaat tcaatcatga gatagtcagc
     3541 agatgagcac ttccaagaac gcagacaagt aagccgcagc aaccttcatt tttcggttgt
     3601 tgcggcgttc tcatgaatcc ttttgctcta cgggagcgcc gccaaatcct ttgttcaagg
     3661 agatggtttc gtgcgctcaa aaaactttag ttggcggtac tcccttgccg ccacggtgtt
     3721 gttgttatca ccgttcgatt tattggcatc actcggcatg gacatgtact tgccagcagt
     3781 gccgtttatg ccaaacgcgc ttggtacgac agcgagcaca attcagctta cgctgacaac
     3841 gtacttggtc atgattggtg ccggtcagct cttgtttgga ccgctatcgg accgactggg
     3901 gcgccgcccc gttctactgg gaggtggcct cgcctacgtt gtggcgtcaa tgggcctcgc
     3961 tcttacgtca tcggctgaag tctttctggg gcttcggatt cttcaggctt gtggtgcctc
     4021 ggcgtgcctt gtttccacat ttgcaacagt acgtgacatt tacgcaggtc gcgaggaaag
     4081 taatgtcatt tacggcatac tcggatccat gctggccatg gtcccggcgg taggcccatt
     4141 gctcggagcg ctcgtcgaca tgtggcttgg gtggcgggct atctttgcgt ttctaggttt
     4201 gggcatgatc gctgcatctg cagcagcgtg gcgattctgg cctgaaaccc gggtgcaacg
     4261 agttgcgggc ttgcaatggt cgcagctgct actccccgtt aagtgcctga acttctggtt
     4321 gtacacgttg tgttacgccg ctggaatggg tagcttcttc gtctttttct ccattgcgcc
     4381 cggactaatg atgggcaggc aaggtgtgtc tcagcttggc ttcagcctgc tgttcgccac
     4441 agtggcaatt gccatggtgt ttacggctcg ttttatgggg cgtgtgatac ccaagtgggg
     4501 cagcccaagt gtcttgcgaa tgggaatggg atgcctgata gctggagcag tattgcttgc
     4561 catcaccgaa atatgggctt cgcagtccgt gttaggcttt attgctccaa tgtggctagt
     4621 gggtattggt gtcgccacag cggtatctgt gtcgcccaat ggcgctcttc gaggattcga
     4681 ccatgttgct ggaacggtca cggcagtcta cttctgcttg ggcggtgtac tgctaggaag
     4741 catcggaacg ttgatcattt cgctgttgcc gcgcaacacg gcttggccgg ttgtcgtgta
     4801 ctgtttgacc cttgcaacag tcgtgctcgg tctgtcttgt gtttcccgag tgaagggctc
     4861 tcgcggccag ggggagcatg atgtggtcgc gctacaaagt gcggaaagta catcaaatcc
     4921 caatcgttga gagaatgtgg caagctatcg cccaacaaat cgctgcagcc gacccaaaac
     4981 cgctacgcgg tttcggtcgg ctgagctcag gcgttaggcc tcgccgaagc gaaagatcgc
     5041 tacttgaagt gttgacgcct ttgttttaaa gtttcgcgtc tatctttctg attttgttaa
     5101 aaaatcagac tttgattaat catgttagac gtcagaggta ttttgactta aacgcctctg
     5161 aggtcgaatt aacgttagcc accaagaagg tgccatgaaa acatttgccg catatgtaat
     5221 tatcgcgtgt ctttcgagta cggcattagc tggttcaatt acagaaaata cgtcttggaa
     5281 caaagagttc tctgccgaag ccgtcaatgg tgtcttcgtg ctttgtaaaa gtagcagtaa
     5341 atcctgcgct accaatgact tagctcgtgc atcaaaggaa tatcttccag catcaacatt
     5401 taagatcccc aacgcaatta tcggcctaga aactggtgtc ataaagaatg agcatcaggt
     5461 tttcaaatgg gacggaaagc caagagccat gaagcaatgg gaaagagact tgaccttaag
     5521 aggggcaata caagtttcag ctgttcccgt atttcaacaa atcgccagag aagttggcga
     5581 agtaagaatg cagaaatacc ttaaaaaatt ttcctatggc aaccagaata tcagtggtgg
     5641 cattgacaaa ttctggttgg aaggccagct tagaatttcc gcagttaatc aagtggagtt
     5701 tctagagtct ctatatttaa ataaattgtc agcatctaaa gaaaaccagc taatagtaaa
     5761 agaggctttg gtaacggagg cggcacctga atatctagtg cattcaaaaa ctggtttttc
     5821 tggtgtggga actgagtcaa atcctggtgt cgcatggtgg gttgggtggg ttgagaagga
     5881 gacagaggtt tactttttcg cctttaacat ggatatagac aacgaaagta agttgccgct
     5941 aagaaaatcc attcccacca aaatcatgga aagtgagggc atcattggtg gctaaaacaa
     6001 agttaaacat catgagggaa gtggtgatcg ccgaagtatc gactcaacta tcagaggtag
     6061 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     6121 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     6181 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     6241 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     6301 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     6361 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     6421 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     6481 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     6541 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     6601 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     6661 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     6721 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg aaaggcgaga
     6781 tcaccaaggt agtcggcaaa taatgtctaa ctcaagcgtt aacccaggat gagaaccttg
     6841 aaagtatcat tgatggctgc gaaagcgaaa aacggcgtga ttggttgcgg tccagacata
     6901 ccctggtccg cgaaagggga gcagctactt tttaaagcat tgacctacaa tcagtggctt
     6961 ctggtgggtc gcaagacgtt tgaatctatg ggcgcactcc ccaataggaa atacgcggtc
     7021 gttacccgct caggttggac atcaaatgat gacaatgtag ttgtatttca gtcaatcgaa
     7081 gaggccatgg acaggctagc tgaattcacc ggtcacgtta tagtgtctgg tggcggagaa
     7141 atttaccgag aaacattacc catggcctct acgctccact tatcgacgat cgacatcgag
     7201 ccagaggggg atgttttctt cccgagtatt ccaaatacct tcgaagttgt ttttgagcaa
     7261 cactttactt caaacattaa ctattgctat caaatttgga aaaagggtta acaaagctat
     7321 gcaatcgacg gcaaaaagct tcgttcgctt cgcgcactac gccttttccg cgattgatag
     7381 cgacgttatg tgaatattga aatgagcatt ccaaagaaag gaagtagaaa aattatcgtg
     7441 ggcgaaaatg agtttttgtg gctcattagg tcaaaactca catattcgca ggactgttta
     7501 ggtacagaaa tgactgcggt cgttgaaccg gatgccggtc ctcactgatg aagaagtgaa
     7561 caccgtacgg gaatcgtgcc ggcagcttgg agctatcggc cggaacctca accaggtggc
     7621 cagggccttg aacatcgagt tcagggaaag tgacaagctc aagcaagagg ccatcgaaaa
     7681 actggccgaa cggatcgacc agcatttgga ccatgtgtct gagctgttcg ataagacctg
     7741 gagccggtgg cacgattgtc attttcagaa gacgactgca ccagttgatt gggcgtaatg
     7801 gctgttgtgc agccagctcc tgacagttca atatcagaag tgatctgcac caatctcgac
     7861 tatgctcaat actcgtgtgg gctctgttgc aaaaatcgtg aagcttgagc atgcttggcg
     7921 gagattggac ggacggaacg atgacggatt tcaagtggcg ccatttccag ggtgatgtga
     7981 tcctgtgggc ggtgcgctgg tattgtcgct atccgatcag ctatcgcgac cttgaggaaa
     8041 tgctggcgga acgcggcatt tcggtcgacc atacgacgat ctatcgctgg gtccagtgct
     8101 acgccccgga gatggagaag cggctgcgct ggttctggcg gcgtggcttt gatccgagct
     8161 ggcgcctgga tgaaacctac gtcaaggtgc ggggcaagtg gacctacctg taccgggcag
     8221 tcgacaagcg gggcgacacg atcgatttct acctgtcgcc gacccgcagc gccaaggcag
     8281 cgaagcggtt cctgggcaag gccctgcgag gcctgaagca ctgggaaaag cctgccacgc
     8341 tcaataccga caaagcgccg agctatggtg cagcgatcac cgaattgaag cgcgaaggaa
     8401 agctggaccg ggagacggcc caccggcagg tgaagtatct caataacgtg atcgaggccg
     8461 atcacggaaa gctcaagata ctgatcaagc cggtgcgcgg tttcaaatcg atccccacgg
     8521 cctatgccac gatcaaggga ttcgaagtca tgcgagccct gcgcaaagga caggctcgcc
     8581 cctggtgcct gcagcccggc atcaggggcg aggtgcgcct tgtggagaga gcttttggca
     8641 ttgggccctc ggcgctgacg gaggccatgg gcatgctcaa ccaccatttc gcagcagccg
     8701 cctgatcggc gcagagcgac agcctacctc tgactgccgc caatctttgc aacagagccc
     8761 gccgtgctag tctgctcggt gatggtggag tgaagccaac ccgcaatcgg gttatgaatc
     8821 tgcatcgcga ttcgcaatca gctgtctctt gagcatgtcg aactcctgcg atgttatctc
     8881 gccgtgctcg tgctgctaca ccaacttgtc caactcatcc gccacaccgc tccccaccac
     8941 ctgcgatggc caggttgttt gctgggcgca ttggcggagt atcgtctcga cccgggacac
     9001 ccacaccggc acaaccttac gggagtgatt cactgtcaaa gaatcggccc ggtgctctga
     9061 cgcaagtatc gggatggtca ccatttgtaa gccgtagacc tgagtggtga tcaagacttc
     9121 gataccaccg accgtaccgg tactaatcga cgacggtcgt gttcgtcgcc tgccgcaggg
     9181 actctgcaca cctccgttta cgcatgtgcc tggaggagtt ggaaatcgtc gtgttcggga
     9241 aacattaaac acaggatggc agcgatctga gccagcacat gatcagctag ctcaccatcc
     9301 ggatcgacgg cccactgcat cgtcgcgcca gcgatgaccg agtgcaggag caactcagct
     9361 gccgcaggag cacctggggg cagtcgcttg cggatcccct ccaccaccgc gcggttccgc
     9421 tggatcgcaa gcgtgcgtag ctccggcacc tggagctcgt accaggagat gagatagttc
     9481 accgagaagt cgttgcgagt gttcatgctc cgaacgagca cctgcaaaaa ttcccagagc
     9541 ccttgcggcc ctgcgcctat cggtatcgca ttcaggtaat gccgcacctg ctcgacgccg
     9601 cgctccatca tcctcaccag cagcgtatcg cggttggtga agcgctggat taacgctgcg
     9661 cgggagagcc ccacctcctt tgctactccg ctgagcgtga actctatggg accgcaacgc
     9721 ttcagcacta cggtggcggc ctcgagtacc tcgtcatcgg acttgagctt ggggcggggc
     9781 atcagtgttc accttctgta tgggttgggg cggaggctgt ggctgccgcc gccattgtag
     9841 caaattgaag acggagcgag agtagagcca cgagccccgc aaacacggcc gatacaacga
     9901 ggccagggag cggaccagca aggtcgacaa acgcgccggc cgcaagcata accatgggcg
     9961 aggctgacag catcaccgcc gagaccgtgc cgagtacccg gccgagaagt tctggcggcg
    10021 tgcggttgta gatggcagcg ttgagaatgg gagagactga gccggtcagc agtcccacga
    10081 gcgcgcccaa caacatcagc accggcacgc ctggcaactg tgaaagcaga agcgagccca
    10141 ccgcagagcc acaaaatgcc accgccagcc agttctgcgc tgatatccgg gcgccgaccg
    10201 acgcatgaat ggcaatgcca aggagaccac cagcccccat cattgaggag aacagcccga
    10261 gctctgctac ttggcgtcct gcatctacaa acagcgcagg catgatgacg ctgccgttgg
    10321 cgccaacgat gcccacgaag atcatcacta taccaaagag agggcgcagc aggggttcgc
    10381 tccagagaaa agcgacgccg gcgcgcatgg agagagtcgc cgtcgtggtc atcgtccgag
    10441 cggcacgcgc gggaagcacc cacgcgccga gcagacctgc aaggacggag cagaacgccg
    10501 tcagcccgag cgttggcgca gcgccaagca ggccgattgc ggccccccaa gggccgggcc
    10561 acctagaatc gcgacgttcc cgatcaccgc tttcagtgac gagacgcgct caacggagag
    10621 cccggcgacg tggccgagtt tgggcagctc actgtcctgc gcggccatac cgggtgcgtc
    10681 gaacgcggca ccgagcacca cgcaagcgat cagcccagtg ttcgagaggg cgccaacggc
    10741 atcgagcagt gggatgctcg ccatggccac gccgcccacc acacccgaga tcaatgcgac
    10801 gggcgcgcgc ccgaaccgat cgacgaggcc accaccaacc cacgcgccga tgatggtcgc
    10861 gatgacgctg ctagcggccg tggcgcccgc ccaggccgcg ctctttgtat gagacaggac
    10921 gaaccatgga agcgcgaggg ccgccaccgc gttgccgatc cggaagagaa aggtcgccgc
    10981 gaacagcgtc gcgagcgggc tatatcgacg ttcgctcatt ccgctgcggc gagctgcgcc
    11041 ttcgccgcag cgaggtactc ttcgttaccc gagtcgaggg cgaagagtgc gtaggtgacc
    11101 gccccgaacg caaggcgctc cgcgatgtgg tgggcgagcc gcggccacac ccggccaccg
    11161 gccgcttcat acgtgaggag gagcttcgcg agcccctctt caccaaagac cataaggtgc
    11221 gcggccatgt cgatggcagg gtcatcaacg cgggcctcgc tccagtcgat catcccgctg
    11281 acgcgctccg tgttgtcgat gagcacatgg cccacgtaga gatcgccatg caccaccacg
    11341 gagaaatctg gccacgacga atcgtcgtcg agccagcgct gccaccggtg gaggcgcttg
    11401 tcgttcacca cgaactcgcg tcggacgcgg tcaacgtcgt cggccacctt ctgacgggcc
    11461 tgcgtcggtg tacggatgag catccccgca tccacggcgg cggaaatggg gacggcatgc
    11521 agggcggcga gcgcggtcgc gaagctctcc gcgaagacct ccgagtcctg cggcacgacc
    11581 cagtcgggcg tggacgaacc aggctggatg accatcgcag tcgagtcttc gagcatggga
    11641 taggcaacga gctcggcgtt ggccacgcgc cagtccggca ccgcgaacgg caggcgattc
    11701 ttgagcattg ccagcacccg cgcctctggt tcgaccttcg cgcttacctc ggctcggcgc
    11761 gggatgcgca gcacccaccg acgtccatcg tcgacggtgg cgatcacgat cctatagtcg
    11821 agcccaagct cattgacagt cagcgggcca tggagcttga gcccatgtcg ggctgcaagt
    11881 gcgtacagtt gggaggtatc ggcggtcgtg actacggtca tgattcactc ctgagggctt
    11941 gacgggttta gccacctaaa tgtaacagtc acgtcggtta tattcaatcc ggcactgttg
    12001 caaagttagc gatgaggcag ccttttgtct tattcaaagg ccttacattt caaaaactct
    12061 gcttaccagg cgcatttcgc ccaggggatc accataataa aatgctgagg cctggccttt
    12121 gcgtagtgca cgcatcacct caataccttt gatggtggcg taagccgtct tcatggattt
    12181 aaatcccagc gtggcgccga ttatccgttt cagtttgcca tgatcgcatt caatcacgtt
    12241 gttccggtac ttaatctgtc ggtgttcaac gtcagacggg caccggcctt cgcgtttgag
    12301 cagagcaagc gcgcgaccat aggcgggcgc tttatccgtg ttgatgaatc gcgggatctg
    12361 ccacttcttc acgttgttga ggattttacc cagaaaccgg tatgcagctt tgctgttacg
    12421 acgggaggag agataaaaat cgacagtgcg gccccggctg tcgacggccc ggtacagata
    12481 cgcccagcgg ccattgacct tcacgtaggt ttcatccatg tgccacgggc aaagatcgga
    12541 agggttacgc cagtaccagc gcagccgttt ttccatttca ggcgcataac gctgaaccca
    12601 gcggtaaatc gtggagtgat cgacattcac tccgcgttca gccagcatct cctgcagctc
    12661 acggtaactg atgccgtatt tgcagtacca gcgtacggcc cacagaatga tgtcacgctg
    12721 aaaatgccgg cctttgaatg ggttcatgtg cagctccatc agcaaaaggg gatgataagt
    12781 ttatcaccac cgactatttg caacagtgcc gtatggtcta tctgcacctg ctccagcggc
    12841 gcggtcacgg caggaggctc gccgcccaca ccttgtaggt cacgagcggc atcctggcct
    12901 tcccgccggc ggatgacctt gcgcgggtca aggctagcga tccgtaaggc cacggtattg
    12961 cgcgccggca ctcgcagttt ttgagccttg cacacctgag tgacttcgcg gtgaaaggcc
    13021 gctaggctgc gcttctgctt ggtcaggaac cgcttttgca gtagctcgtg gatgacgcgc
    13081 tcgaccggtt ccggcaagcg ccccttacct ttacctccac cggactggcc gggcaccaga
    13141 tccgtcacga ggccgctgcc ttgccgggca cgccggatca gaacgtatac ctggcgccga
    13201 gacaagccca gcgcctgagc cgccatatcg gccgcttcgt gcccgaccgt ctccgactgc
    13261 gccaacggac tgatgatctc cgcacgacgg cgcgcacgct cccaagcctc atcaggcaga
    13321 gtggccacgc cttgttctgg aatccgtggg gtgtccgtcg ccatgctcac ctcgctttgg
    13381 tgcacacgag tattgagcat agtcgagatt ggtgcagatc acttctgata ttgaactgtc
    13441 aggagctggc tgcacaacag ccattacgcc caatcaactg gtgcagtcgt cttctgaaaa
    13501 tgaca
//
