LOCUS       In1079                 15929 bp    DNA     linear       29-SEP-2020
DEFINITION  Pseudomonas aeruginosa.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Unclassified.
REFERENCE   1  (bases 1 to 15929)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of In1079
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15929)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..15929
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
     repeat_region   1..25
                     /gene="IRi_In1079"
     CDS             complement(203..1216)
                     /gene="intI1"
                     /codon_start=1
                     /product="Integrase"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVNWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     CDS             1363..1917
                     /gene="aacA4-12"
                     /codon_start=1
                     /product="Aminoglycoside 6'-N-acetyltransferase"
                     /translation="-
                     TNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLADVQEQYLPSVLAQES
                     VTPYIAMLNGEPIGYAQSYVALGGGDGWWEEETDPGVRGIDQLLANASQLGKGLGTKL
                     VRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFERQGTVTTPDGPAVYMVQTRQ
                     AFERTRSDA"
     misc_recomb     1912..1983
                     /gene="attC_aacA4-12"
     CDS             1998..2798
                     /gene="blaOXA-101"
                     /codon_start=1
                     /product="Beta-lactamase blaOXA-101"
                     /translation="MKTFAAYVITACLSSTALASSITENTFWNKEFSAEAVNGVFVLC
                     KSSSKSCATNNLARASKEYLPASTFKIPNAIIGLETGVIKNEHQVFKWDGKPRAMKQW
                     ERDLSLRGAIQVSAVPVFQQIAREVGEVRMQKYLKKFSYGNQNISGGIDKFWLEGQLR
                     ISAVNQVEFLESLFLNKLSASKENQLIVKEALVTEAAPEYLVHSKTGFSGVGTESNPG
                     VAWWVGWVEKGTEVYFFAFNMDIDNENKLPLRKSIPTKIMASEGIIGG"
     misc_recomb     2793..2856
                     /gene="attC_blaOXA-101"
     CDS             2860..3648
                     /gene="aadA5"
                     /codon_start=1
                     /product="Aminoglycoside 3'-nucleotidyltransferase"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     misc_recomb     3695..3707
                     /gene="truncated attC_aadA5"
     CDS             3816..4163
                     /gene="qacED1"
                     /codon_start=1
                     /product="Quaternary ammonium compound resistance protein"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     CDS             4157..4996
                     /gene="sul1"
                     /codon_start=1
                     /product="Dihydropteroate synthase"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     CDS             5401..6942
                     /gene="tnpA"
                     /codon_start=1
                     /product="ISCR1 transposase"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASL
                     EAQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGAR
                     RMAESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIK
                     KAGYTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYD
                     ELNTLAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGP
                     QQGRKVFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPA
                     VSEKRLALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVF
                     APNSKHRVQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVC
                     EHCGGHVKVIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQ
                     SRLFD"
     repeat_region   7154..7174
                     /gene="oriIS"
     repeat_region   7182..7202
                     /gene="oriIS"
     CDS             7265..8191
                     /gene="blaPER-1"
                     /codon_start=1
                     /product="Extended-spectrum beta-lactamase PER-1"
                     /translation="MNVIIKAVVTASTLLMVSFSSFETSAQSPLLKEQIESIVIGKKA
                     TVGVAVWGPDDLEPLLINPFEKFPMQSVFKLHLAMLVLHQVDQGKLDLNQTVIVNRAK
                     VLQNTWAPIMKAYQGDEFSVPVQQLLQYSVSHSDNVACDLLFELVGGPAALHDYIQSM
                     GIKETAVVANEAQMHADDQVQYQNWTSMKGAAEILKKFEQKTQLSETSQALLWKWMVE
                     TTTGPERLKGLLPAGTVVAHKTGTSGIKAGKTAATNDLGIILLPDGRPLLVAVFVKDS
                     AESSRTNEAIIAQVAQTAYQFELKKLSALSPN"
     CDS             8265..8840
                     /codon_start=1
                     /product="Maleylacetoacetate isomerase / Glutathione
                     S-transferase"
                     /translation="MQLLGSVASPFVRRLRLVLAGQPYQFVALNIFESEGRSVLVQHN
                     PARKVPVLVDGEQVIFDSGVIYRYLASKLKFKPLSWDQENGLTTINACTDSLVELLLC
                     KRSGFDVTEDKLFFNLQHERIQATLEALEQQIRAGHFGDWDYPGISLFTLIDWILFRD
                     LVDLKPFPVLLQFRNAHLNRPMVAETDPRLS"
     CDS             complement(8879..10720)
                     /gene="mdlB"
                     /codon_start=1
                     /product="ABC MDR transporter/ATP-binding cassette (ABC)
                     transporters"
                     /translation="MLGFFERLTNPFPDKEPTQPPQGLFAFCLHYSQGMVLPLILMSI
                     ATALLAALEVSLFGYMGQLVDWLVTKNPATFWQEEQDTLITMAIVMLLVLPGLVFFHS
                     ALIHQTILGNYPMSIRWLAHRYLLKQSLGFYQNEFAGRIATKVMQTALAVRETATKLL
                     DVMVYVVVYFGSMLYLIADADWRLVLPMIAWLVIYTGLQFYFVPRLKQVATEQADARS
                     EMTGRIVDSYTNISTIKLFSHTARESEYARDSMQLFLVPVYRQMRLATWLNICVQGLN
                     YLLVFSIATLAIWLWSVNAVTVGSIAIAVSLALRLNGMSQWIMWEVSALFENIGTVAD
                     GIATLSQPQQIIDKADAKPLEVPQGQIEFKQLNFNYGKAAGEHGPVIDGLDLSIKPGE
                     KVGLVGRSGAGKSTLVNLLLRFYDVNSGQILIDGQDIKTVSQESLRAHIAMVTQDTSL
                     LHRTVRENILYGRPGATEAEMIEAARLAEADAFIQELTDPKGNSGYDAQVGERGVKLS
                     GGQRQRIAIARVLLKNAPILILDEATSALDSEVESAIQHSLTQLMIGKTVIAIAHRLS
                     TIAAMDRLIVLDQGRIVEQGSHQELIAKGGIYAQLWAHQTGGFIGVE"
     CDS             10840..10962
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="-TRFFAEQNSRLLKINTKNLKNPKDAISHELSSLDLLKDR"
     CDS             11091..11792
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MDAKELLVYMKQAELQAELVVEAGQQLDRAIKQLHDWSEDWDCQ
                     RRACEDMYDAVHLILIHSYQLSRIFWPAKCCGELGLDLCHRAMALRSEINLPDLNHPL
                     RNEALWRHADELDHSVRDNTALRRYQAHHLTGFNQVITWMDNEAMFCWLEPASKTFVF
                     HAEEFALAQLLDAVHQVQQDIQNFLNKQHQSNLKVAKAGVHHYEKQWGAFKIRQDRSC
                     PQLCCYDYLSNLINP"
     CDS             complement(11765..12094)
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MLTFVMSAITFGFLLLSLFFYKKLIGMSDALNIIEKQVAADMEI
                     RAHRLCLLAYEAQRFGNSVDRRALDEEFKDFLHLYIEDYQAEVAKKIREHKLSEISAY
                     GFIKLDK"
     CDS             complement(12593..12823)
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MQINLNTPGTAATSTIQTVAPAAQAKTTTTTAPSTADTVTLSAE
                     AKAKLAAEQQESKAINPTLTTLGGGWGNEPPK"
     misc_feature    12859..13143
                     /gene="truncated qacED1"
                     /product="Truncated quaternary ammonium coumpound
                     resistance protein (pseudogene)"
     CDS             13137..13976
                     /gene="sul1"
                     /codon_start=1
                     /product="Dihydropteroate synthase"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     CDS             14104..14604
                     /gene="orf5"
                     /codon_start=1
                     /product="Putative acetyltransferase"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     CDS             14628..14915
                     /gene="orf6"
                     /codon_start=1
                     /product="Hypothetical protein"
                     /translation="MSGWDGPVASRSLAVRPRCLGCARGCRYPPLPSSTLDNAAAVIA
                     ADARAGLFGPVRLARPSACLGEISGGRINGGASPPFVRVKAEDSSLKNIRL"
     repeat_region   complement(14927..14951)
                     /gene="IRt_In1079"
     repeat_region   15050..15063
                     /gene="IRL_IS6100"
     CDS             15081..15875
                     /gene="tnpA"
                     /codon_start=1
                     /product="IS6100 transposase"
                     /translation="MLGGDWTDGTMTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEM
                     LAERGISVDHTTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYR
                     AVDKRGDTIDFYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELK
                     REGKLDRETAHRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALR
                     KGQARPWCLQPGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     repeat_region   15916..15929
                     /gene="IRR_IS6100"
ORIGIN      
        1 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
       61 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
      121 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
      181 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
      241 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
      301 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
      361 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
      421 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
      481 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
      541 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
      601 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
      661 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
      721 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
      781 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
      841 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
      901 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
      961 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     1021 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     1081 ggatgaaggc acgaacccag ttgacataag cctgttcggt tcgtaaactg taatgcaagt
     1141 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1201 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat
     1261 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1321 ttacgcagta aaacaaagtt aggcatcaca aagtacagca tcgtgaccaa cagcaacgat
     1381 tccgtcacac tgcgcctcat gactgagcat gaccttgcga tgctctatga gtggctaaat
     1441 cgatctcata tcgtcgagtg gtggggcgga gaagaagcac gcccgacact tgctgacgta
     1501 caggaacagt acttgccaag cgttttagcg caagagtccg tcactccata cattgcaatg
     1561 ctgaatggag agccgattgg gtatgcccag tcgtacgttg ctcttggagg cggggacgga
     1621 tggtgggaag aagaaaccga tccaggagta cgcggaatag accagttact ggcgaatgca
     1681 tcacaactgg gcaaaggctt gggaaccaag ctggttcgag cactggttga gttgctgttc
     1741 aatgatcccg aggtcaccaa gatccaaacg gacccgtcgc cgagcaactt gcgagcgatc
     1801 cgatgctacg agaaagcggg gtttgagagg caaggtaccg taaccacccc agatggtcca
     1861 gccgtgtaca tggttcaaac acgccaggca ttcgagcgaa cacgcagtga tgcctaaccc
     1921 ttccatcgag ggggacgtcc aagggctggc gcccttggtc gcccctcatg tcaaacgtta
     1981 gccaccaaga aggtgccatg aaaacatttg ccgcatatgt aattactgcg tgtctttcaa
     2041 gtacggcatt agctagttca attacagaaa atacgttttg gaacaaagag ttctctgccg
     2101 aagccgtcaa tggtgttttc gtgctttgta aaagtagcag taaatcctgc gctaccaata
     2161 acttagctcg tgcatcaaag gaatatcttc cagcatcaac atttaagatc cccaacgcaa
     2221 ttatcggcct agaaactggt gtcataaaga atgagcatca ggttttcaaa tgggacggaa
     2281 agccaagagc catgaaacaa tgggaaagag acttgagctt aagaggggca atacaagttt
     2341 cagcggttcc cgtatttcaa caaatcgcca gagaagttgg cgaagtaaga atgcagaaat
     2401 atcttaaaaa attttcatat ggtaaccaga atatcagtgg tggcattgac aaattctggt
     2461 tggagggtca gcttagaatt tccgcagtta atcaagtgga gtttctagag tctctatttt
     2521 taaataaatt gtcagcatca aaagaaaatc agctaatagt aaaagaggct ttggtaacgg
     2581 aggctgcgcc tgaatatctt gtgcattcaa aaactggttt ttctggtgtg ggaactgagt
     2641 caaatcctgg tgtcgcatgg tgggttggtt gggttgagaa gggaacagag gtttactttt
     2701 tcgcctttaa catggatata gacaacgaaa ataagttgcc gctaagaaaa tccattccca
     2761 ccaaaatcat ggcaagtgag ggcatcattg gtggctaaca attcgctgca ggcgcgacgg
     2821 ccctgacggg ccgcggcctg agctcaaacg ttaggcatca tgggtgaatt tttccctgca
     2881 caagttttca agcagctgtc ccacgctcgc gcggtgatcg agcgccatct ggctgcgaca
     2941 ctggacacaa tccacctgtt cggatctgcg atcgatggag ggctgaagcc ggacagcgac
     3001 atagacttgc tcgtgaccgt cagcgccgca cctaacgatt cgctccggca ggcgctaatg
     3061 ctcgatttgc tgaaagtctc atcaccgcca ggcgatggcg gaacatggcg accgctggag
     3121 ctaactgttg tcgctcgaag cgaagtagtg ccttggcgct atccggcgcg gcgtgagctt
     3181 cagttcggtg agtggctccg ccacgacatc ctttccggaa cgttcgagcc tgccgttctg
     3241 gatcacgatc ttgcgatttt gctgaccaag gcgaggcaac acagccttgc gcttctaggc
     3301 ccatccgcag ccacgttttt cgagccggtg ccgaaggagc atttctccaa ggcgcttttc
     3361 gacactattg cccagtggaa tgcagagtcg gattggaagg gtgacgagcg gaacgtcgtt
     3421 cttgctcttg ctcgcatttg gtacagcgct tcaactggtc tcattgctcc taaggacgtt
     3481 gctgccgcat gggtatcgga gcgtttgcct gccgagcatc ggcccctcat ctgcaaggca
     3541 cgcgcggcgt acctgggtag cgaggacgac gacctagcaa tgcgcgtcga agagacggcc
     3601 gcgttcgttc gatatgccaa agcaacgatt gagagaatct tgcgttgagc ggcatgtgcg
     3661 aaaagtgcat cgacccgcgc cgagggcatc tgatgcctaa ctatgcgtta gatgcactaa
     3721 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     3781 gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt tttcttgtta
     3841 tcgcaatagt tggcgaagta atcgcaacat ccgcattaaa atctagcgag ggctttacta
     3901 agcttgcccc ttccgccgtt gtcataatcg gttatggcat cgcattttat tttctttctc
     3961 tggttctgaa atccatccct gtcggtgttg cttatgcagt ctggtcggga ctcggcgtcg
     4021 tcataattac agccattgcc tggttgcttc atgggcaaaa gcttgatgcg tggggctttg
     4081 taggtatggg gctcataatt gctgcctttt tgctcgcccg atccccatcg tggaagtcgc
     4141 tgcggaggcc gacgccatgg tgacggtgtt cggcattctg aatctcaccg aggactcctt
     4201 cttcgatgag agccggcggc tagaccccgc cggcgctgtc accgcggcga tcgaaatgct
     4261 gcgagtcgga tcagacgtcg tggatgtcgg accggccgcc agccatccgg acgcgaggcc
     4321 tgtatcgccg gccgatgaga tcagacgtat tgcgccgctc ttagacgccc tgtccgatca
     4381 gatgcaccgt gtttcaatcg acagcttcca accggaaacc cagcgctatg cgctcaagcg
     4441 cggcgtgggc tacctgaacg atatccaagg atttcctgac cctgcgctct atcccgatat
     4501 tgctgaggcg gactgcaggc tggtggttat gcactcagcg cagcgggatg gcatcgccac
     4561 ccgcaccggt caccttcgac ccgaagacgc gctcgacgag attgtgcggt tcttcgaggc
     4621 gcgggtttcc gccttgcgac ggagcggggt cgctgccgac cggctcatcc tcgatccggg
     4681 gatgggattt ttcttgagcc ccgcaccgga aacatcgctg cacgtgctgt cgaaccttca
     4741 aaagctgaag tcggcgttgg ggcttccgct attggtctcg gtgtcgcgga aatccttctt
     4801 gggcgccacc gttggccttc ctgtaaagga tctgggtcca gcgagccttg cggcggaact
     4861 tcacgcgatc ggcaatggcg ctgactacgt ccgcacccac gcgcctggag atctgcgaag
     4921 cgcaatcacc ttctcggaaa ccctcgcgaa atttcgcagt cgcgacgcca gagaccgagg
     4981 gttagatcat gcctagcatt caccttccgg ccgcccgcta aatatctcct tttgggttgt
     5041 taataaaaca tccaataagt tgactgtgcg tgaaaaagaa agttttgtgt gatggcgttg
     5101 aagatcgcac cgttaagctc ttatgtggga tggtgcagag ctcgacgact accgataaaa
     5161 cgcaaccgcc gcaaacagac aagaaaaagc cccaactgat aacagttggg gcttcagtat
     5221 tgtgattggt ggagcaatag caccctgaac ccaaaacctt ctcgctcaac cggtagtggc
     5281 tgataacaac tcgtgagggc tattgcgggt taagcattta gcgatgtcta gggccagact
     5341 ggacgtctga acgcaagccg ctgatactgt acataaccac agtatcagcg gaggataccc
     5401 atgtcgctgg caaggaacgc cacggcgagt caatcgccca ctcaaacaaa cggttacgaa
     5461 cgccaccaac ccgaccagac gctgctctac cagctggttg agcagcacta cccagccttc
     5521 aaagcctcac tcgaagccca aggtcaacac ctgcctcgct acatccaaca agaattcaac
     5581 gacctcctcc aatgtggccg tctggagtat ggtttcatgc gggttcgctg cgaggattgt
     5641 catcacgagc gtctggtcgc cttcagctgt aaacgacgcg gcttttgccc tagctgcggt
     5701 gcccgccgga tggccgagag tgcggcgctg ctgatagacg aagtcttccc caaggagccc
     5761 attcgccagt gggtgctcag ctttcctttc cagctacgct ttttgctggc tcgccatccc
     5821 cagctgatgg gccaggtctt gagtatcgtc tatcgtacac tctcaactca tctgatcaaa
     5881 aaagccggtt acaccaaagc ctctgcacaa actggctcag tgactcttat ccaacgcttt
     5941 ggctccgcgc taaatctcaa tgtccactac cacatgctgt ttctcgatgg tgtctatgcc
     6001 gaagatgact atggcaagca acgcttccat cgtgtcaagg cacccactta cgatgagctg
     6061 aatacgctcg ctcacaccct cagccatcgc atcgctcgct gcatggaaaa gcgtgggatt
     6121 ttggagcgtg atgccgagaa tacgtggttg acactggaag agggcgaaga cgatacgctg
     6181 actcaattac atggtgcttc ggttacgtat cgcattgccg tcggccccca gcaagggcgc
     6241 aaagtcttca ccctgcaaac cttgccaggg cgtgaggata aagccgactc aagcagtcga
     6301 gtagccaacc atgctggttt ctcgctacac gccggtgtga tggccgaagc gcatcagcgg
     6361 gataagcttg agcgcttgtg tcgctacatt agtcggccag cggtttcaga aaaacgtctg
     6421 gcattaaccg ccaatgggca ggtgcgttac gagctcaaaa ctccgtaccg caatggcacc
     6481 acccatgtga tcttcgagcc gctggacttc atcgccaaac tcgctgcgtt ggtacctaag
     6541 ccgcgagtca acctcacacg cttccacggc gtctttgcac cgaacagcaa acaccgagtt
     6601 caagtaacac ccgccaagcg gggcaagaag cccgacaaat cggaaggtct cgatactaac
     6661 tggcgtgaca agagtcctgc agagcgccac cgcgccatga cctggatgca acgcctcaag
     6721 cgagtcttca atattgatat tgaagtctgc gaacactgcg gcggtcacgt caaagtgatt
     6781 gccagcatcg aagatccgaa ggtcattgag cagattctca agcatctgaa acagaaaaca
     6841 gccaaggcga atgccgccaa gcagcgtgag ctgccaccag aacgagcgcc gccactgact
     6901 cccagcctgt tcgatccatc acagagtcgt ctctttgact gacgacccca aatccaacac
     6961 tgctcaacac tgccaacttt taaacggggc ggtggggcag tttgtatctc tcgagctatc
     7021 aggctagaga ttttaccgcc aaatcgaacc ttattagagc ggtttaggct ggaccggcag
     7081 ttaaaattgg ggcttgagcg gtaaacgagt gagggaattt caggtaagat acttcggatg
     7141 aggagcaaaa aggtggttta tacttcctat acccaaaaag gtggtttata cttcctatac
     7201 cctaaaaagg cagctttggc tgccttttgt ataattcata tcgacttatc aaaaggacaa
     7261 tccgatgaat gtcattataa aagctgtagt tactgcctcg acgctactga tggtatcttt
     7321 tagttcattc gaaacctcag cgcaatcccc actgttaaaa gagcaaattg aatccatagt
     7381 cattggaaaa aaagccactg taggcgttgc agtgtggggg cctgacgatc tggaaccttt
     7441 actgattaat ccttttgaaa aattcccaat gcaaagtgta tttaaattgc atttagctat
     7501 gttggtactg catcaggttg atcagggaaa gttggattta aatcagaccg ttatcgtaaa
     7561 cagggctaag gttttacaga atacctgggc tccgataatg aaagcgtatc agggagacga
     7621 gtttagtgtt ccagtgcagc aactgctgca atactcggtc tcgcacagcg ataacgtggc
     7681 ctgtgatttg ttatttgaac tggttggtgg accagctgct ttgcatgact atatccagtc
     7741 tatgggtata aaggagaccg ctgtggtcgc aaatgaagcg cagatgcacg ccgatgatca
     7801 ggtgcagtat caaaactgga cctcgatgaa aggtgctgca gagatcctga aaaagtttga
     7861 gcaaaaaaca cagctgtctg aaacctcgca ggctttgtta tggaagtgga tggtcgaaac
     7921 caccacagga ccagagcggt taaaaggttt gttaccagct ggtactgtgg tcgcacataa
     7981 aactggtact tcgggtatca aagccggaaa aactgcggcc actaatgatt taggtatcat
     8041 tctgttgcct gatggacggc ccttgctggt tgctgttttt gtgaaagact cagccgagtc
     8101 aagccgaacc aatgaagcta tcattgcgca ggttgctcag actgcgtatc aatttgaatt
     8161 gaaaaagctt tctgccctaa gcccaaatta acagactatc agcactatct aagccgctga
     8221 ctctggttgt acactaaagc tatggtttga aattggagta ggttatgcag ttattaggtt
     8281 cagtggcttc cccttttgtt cgtcgtttac gtttagtact ggcagggcaa ccttatcagt
     8341 ttgtagcgct taatattttt gagtctgaag gccgttcagt gttggtacaa cataatccgg
     8401 cacgcaaagt gcctgtgtta gtggatggag agcaggttat ttttgattca ggcgtaattt
     8461 atcgttattt ggcttcgaaa ctgaaattca aaccattgag ctgggatcag gaaaacggtc
     8521 tgacgaccat caatgcctgc acagactcct tggttgaatt actgctttgt aagcgctcag
     8581 gttttgacgt gactgaagat aaattgtttt ttaatcttca gcatgagcga attcaggcga
     8641 cgctagaggc acttgagcaa caaatcaggg cagggcattt cggtgactgg gactatcctg
     8701 gtatcagtct gttcacttta atcgactgga tattgttccg cgatttagtc gacttaaaac
     8761 ccttccctgt attattacag ttcagaaatg cgcatttaaa ccggccgatg gtcgctgaaa
     8821 ccgacccgcg tttaagctaa aaacaacagg tcagagccaa gactctgacc tttattattt
     8881 attccacacc aataaagccg ccggtttgat gcgcccataa ttgagcgtaa ataccgcctt
     8941 tggcgatcag ttcctgatga ctgccttgtt ccacaatacg accttgatcc agaacgatca
     9001 gcctgtccat agctgctatg gtagataaac ggtgtgcaat ggctataacc gttttgccta
     9061 tcatcaactg agtcagacta tgctgaatag ctgattctac ttcggagtcc agcgctgagg
     9121 tggcttcatc caaaatcaga ataggggcgt ttttcagtaa aacccgagct attgcaatac
     9181 gttgacgttg accaccagaa agcttgactc cacgttcgcc cacctgagcg tcgtagccac
     9241 tgttgccttt aggatcggta agctcctgaa taaaagcatc ggcttcggct aaacgggctg
     9301 cttcaatcat ttccgcttcg gtagcgccag gacgaccata taaaatattt tcccttacag
     9361 tacggtgtag cagggaggta tcctgggtca ccatagctat atgagcgcga agactttcct
     9421 gtgatacagt tttgatgtcc tgaccgtcta tcaaaatctg gcctgaattg acatcataaa
     9481 agcgcagcag taagttcacc agcgtggatt taccagcacc agaacggccg actaaaccta
     9541 ctttttcacc gggtttaatc gacaagtcca gaccatcaat cacaggacca tgttcacctg
     9601 ctgctttgcc gtagttaaag ttcagttgct taaactcaat ctgaccttgt ggcacttcaa
     9661 gcggcttcgc atcagctttg tcgatgattt gctgaggctg agacaaagtc gcaataccat
     9721 ctgccacagt acctatgttt tcaaacagtg cactgacttc ccacatgatc cactgcgaca
     9781 taccatttaa acgcagcgcc agactgactg cgatggcaat agagcccaca gtgacggcat
     9841 tgacagacca aagccatatc gccagcgtgg caatagagaa caccaacaga tagttcagac
     9901 cctgcacaca aatattgagc caggtagcca gtcgcatttg tctgtacaca ggcaccagaa
     9961 acagctgcat gctatcgcgg gcgtattctg actcacgagc cgtgtgtgaa aacagtttaa
    10021 tagtgctgat attggtgtag ctgtcgacaa tacggcctgt catttctgaa cgggcatcgg
    10081 cttgttctgt tgcaacttgt ttcagtctcg gcacaaaata aaactgcaag ccggtgtata
    10141 tgactaacca ggcaatcatc ggcagtacca agcgccaatc tgcatcggcg atcagataca
    10201 acatagagcc aaaatacacc accacataca ccatcacatc cagcagcttg gtggccgttt
    10261 cgcgcactgc caaagccgtt tgcatcactt tagtggcgat gcgaccagcg aattcattct
    10321 ggtagaaacc caaactttgt ttcagtaaat aacgatgcgc cagccagcgg atcgacatag
    10381 ggtagttacc caatatagtt tggtggatca gggctgagtg aaaaaatacc aaaccaggca
    10441 acaccagcaa catcactatg gccatagtga tcaaggtatc ctgctcttcc tgccagaaag
    10501 tagccggatt cttggttacc aaccaatcga ctaattgccc catatagcca aataaggaca
    10561 cttctaatgc ggccagtaag gctgtcgcaa tagacatcag gatcagaggc aacaccatgc
    10621 cttgactgta atgcaaacaa aatgcaaaca ggccttgagg tggctgagtt ggctctttat
    10681 cagggaaagg gttggttaag cgttcaaaaa agcccagcat aaaattcctc tgcagcaacg
    10741 aaaaaggcag ttattgtacg tcaaagttta aaaaacagat gtaactattt gatgaaagcc
    10801 cggtttagaa cggccatctt attttataac cgatagcttg tgactcgctt ttttgctgaa
    10861 cagaacagca gattgttgaa aataaataca aaaaatctga aaaatccaaa agatgctata
    10921 tcacatgaac tcagcagctt agatttattg aaggaccgtt aataggtaat aactggaaaa
    10981 tctataactt agcttctgtt ttgtcagctt gcaaaggact ttttgctttc tacactgaaa
    11041 atacagagta cccgaatcag ggtgttgtct cgtcttacag gagtgttgaa atggatgcca
    11101 aagaattatt ggtctacatg aagcaggcag aactgcaagc tgagttagtc gttgaggctg
    11161 gtcagcaact ggacagagcc atcaaacaat tacatgactg gtctgaagac tgggattgtc
    11221 agcgccgggc ttgtgaagat atgtacgatg ccgtgcattt aattctgatc catagctatc
    11281 aattatcccg gattttctgg cccgccaagt gttgcggaga actggggctg gatttgtgtc
    11341 atagagccat ggcgctgcga agtgaaatca acctgcctga tctaaaccat ccactgcgga
    11401 atgaggcgtt atggcgtcat gcagatgagc tggatcattc agtgcgggat aatactgctc
    11461 tgcgtcgtta tcaagcgcat catttaaccg gttttaatca ggtgatcacc tggatggata
    11521 atgaagccat gttttgctgg cttgaacctg ccagtaaaac ctttgtgttt catgccgagg
    11581 agtttgcttt agcccaatta ctggatgctg tgcatcaggt tcagcaagat attcagaatt
    11641 ttcttaacaa gcagcatcag tcaaatctaa aggtggccaa agctggcgtg catcactatg
    11701 agaagcagtg gggggcattt aaaataagac aagacagatc ttgtccacag ctttgttgtt
    11761 atgactactt atctaattta ataaatccgt aggcagaaat ctcgctgagc ttatgttctc
    11821 tgatcttttt tgcgacttct gcttggtagt cttcaatgta caggtgcaaa aaatctttaa
    11881 actcttcatc caaagcacgt ctatccacag agttaccgaa ccgttgcgct tcgtatgcaa
    11941 gcaggcataa tctatgcgcc cggatctcca tatcagcggc cacctgcttt tcgataatgt
    12001 ttaatgcatc tgacattcca atcaacttct tgtagaaaaa cagacttaac agtaaaaagc
    12061 caaacgtaat tgcagacata acaaaagtca gcattcctga agttcctttc tgtgcttgac
    12121 ggctcctgat atggaccata aaaatatcgc taaaattgct gcgatattta tagctggtat
    12181 tccatatttg tatataccac ccaatagatc agtgccaaat atgactctgt ctgtgaatct
    12241 cgctacctgc agaacagagt gcatcaagat gcttaaacaa atgaattgag tgatttgttc
    12301 aaccgcccat acgaattttc tatgaataac ccagatgata ccaagcgtca atgtttgaca
    12361 tatcacccaa cttgggtacc aaagtaagcg agcgagctca ggagatgttt cagatatccc
    12421 catcagcaaa ggagcaatcc tgtcttgagc taatcccgat aaagtgagta tcaatatggt
    12481 tattcgtgca gatttactgg ccgaccagac taaggacagc agtagagaac ctatgcacaa
    12541 cacccataag tactcagcaa gagtcgtaat aaatgtcccc acaaactacc tcttattttg
    12601 gtggttcatt accccagcca ccacctaatg tagtcagtgt tggatttatt gctttagatt
    12661 cttgctgctc tgcagccagc ttcgcctttg cttcagcaga taaggtgaca gtgtctgcag
    12721 tagagggtgc tgttgtagtg gtggttttag cctgagcagc aggagcaaca gtttgtattg
    12781 ttgatgtagc cgctgtgccc ggagtattta agttgatttg cataactgtt ccttaccatt
    12841 gtcactaaat cagaaaacaa atgtaccgag ggctttacta agcttgcccc ttccgccgtt
    12901 gtcataatcg gttatggcat cgcattttat tttctttctc tggttctgaa atccatccct
    12961 gtcggtgttg cttatgcagt ctggtcggga ctcggcgtcg tcataattac agccattgcc
    13021 tggttgcttc atgggcaaaa gcttgatgcg tggggctttg taggtatggg gctcataatt
    13081 gctgcctttt tgctcgcccg atccccatcg tggaagtcgc tgcggaggcc gacgccatgg
    13141 tgacggtgtt cggcattctg aatctcaccg aggactcctt cttcgatgag agccggcggc
    13201 tagaccccgc cggcgctgtc accgcggcga tcgaaatgct gcgagtcgga tcagacgtcg
    13261 tggatgtcgg accggccgcc agccatccgg acgcgaggcc tgtatcgccg gccgatgaga
    13321 tcagacgtat tgcgccgctc ttagacgccc tgtccgatca gatgcaccgt gtttcaatcg
    13381 acagcttcca accggaaacc cagcgctatg cgctcaagcg cggcgtgggc tacctgaacg
    13441 atatccaagg atttcctgac cctgcgctct atcccgatat tgctgaggcg gactgcaggc
    13501 tggtggttat gcactcagcg cagcgggatg gcatcgccac ccgcaccggt caccttcgac
    13561 ccgaagacgc gctcgacgag attgtgcggt tcttcgaggc gcgggtttcc gccttgcgac
    13621 ggagcggggt cgctgccgac cggctcatcc tcgatccggg gatgggattt ttcttgagcc
    13681 ccgcaccgga aacatcgctg cacgtgctgt cgaaccttca aaagctgaag tcggcgttgg
    13741 ggcttccgct attggtctcg gtgtcgcgga aatccttctt gggcgccacc gttggccttc
    13801 ctgtaaagga tctgggtcca gcgagccttg cggcggaact tcacgcgatc ggcaatggcg
    13861 ctgactacgt ccgcacccac gcgcctggag atctgcgaag cgcaatcacc ttctcggaaa
    13921 ccctcgcgaa atttcgcagt cgcgacgcca gagaccgagg gttagatcat gcctagcatt
    13981 caccttccgg ccgcccgcta gcggaccctg gtcaggttcc gcgaaggtgg gcgcagacat
    14041 gctgggctcg tcaggatcaa actgcactat gaggcggcgg ttcataccgc gccaggggag
    14101 cgaatggaca gcgaggagcc tccgaacgtt cgggtcgcct gctcgggtga tatcgacgag
    14161 gttgtgcggc tgatgcacga cgctgcggcg tggatgtccg ccaagggaac gcccgcctgg
    14221 gacgtcgcgc ggatcgaccg gacattcgcg gagaccttcg tcctgagatc cgagctccta
    14281 gtcgcgagtt gcagcgacgg catcgtcggc tgttgcacct tgtcggccga ggatcccgag
    14341 ttctggcccg acgccctcaa gggggaggcc gcatatctgc acaagctcgc ggtgcgacgg
    14401 acacatgcgg gccggggtgt cagctccgcg ctgatcgagg cttgccgcca tgccgcgcga
    14461 acgcaggggt gcgccaagct gcggctcgac tgccacccga acctgcgtgg cctatacgag
    14521 cggctcggat tcacccacgt cgacactttc aatcccggct gggatccaac cttcatcgca
    14581 gaacgcctag aactcgaaat ctaacgtccg ttcgggcatc gaggtccatg tcggggtggg
    14641 acgggcccgt ggcttcaaga tcacttgcag tccgaccgcg atgtcttggt tgcgcgagag
    14701 gttgtcgata tcctccactt ccatcatcaa ccctggataa tgccgccgcc gtcatcgccg
    14761 ccgacgcccg tgccgggctt ttcgggcctg tcaggcttgc tcggccttca gcctgcctgg
    14821 gcgagatctc cggcggacgg attaacggcg gagcttcgcc gcctttcgtg cgtgtgaagg
    14881 ccgaagatag ttctctcaaa aacatccgtt tatgagagat accaaatgtc attttcagaa
    14941 gacgactgca ccagttgatt gggcgtaatg gctgttgtgc agccagctcc tgacagttca
    15001 atatcagaag tgatctgcac caatctcgac tatgctcaat actcgtgtgg gctctgttgc
    15061 aaaaatcgtg aagcttgagc atgcttggcg gagattggac ggacggaacg atgacggatt
    15121 tcaagtggcg ccatttccag ggtgatgtga tcctgtgggc ggtgcgctgg tattgtcgct
    15181 atccgatcag ctatcgcgac cttgaggaaa tgctggcgga acgcggcatt tcggtcgacc
    15241 atacgacgat ctatcgctgg gtccagtgct acgccccgga gatggagaag cggctgcgct
    15301 ggttctggcg gcgtggcttt gatccgagct ggcgcctgga tgaaacctac gtcaaggtgc
    15361 ggggcaagtg gacctacctg taccgggcag tcgacaagcg gggcgacacg atcgatttct
    15421 acctgtcgcc gacccgcagc gccaaggcag cgaagcggtt cctgggcaag gccctgcgag
    15481 gcctgaagca ctgggaaaag cctgccacgc tcaataccga caaagcgccg agctatggtg
    15541 cagcgatcac cgaattgaag cgcgaaggaa agctggaccg ggagacggcc caccggcagg
    15601 tgaagtatct caataacgtg atcgaggccg atcacggaaa gctcaagata ctgatcaagc
    15661 cggtgcgcgg tttcaaatcg atccccacgg cctatgccac gatcaaggga ttcgaagtca
    15721 tgcgagccct gcgcaaagga caggctcgcc cctggtgcct gcagcccggc atcaggggcg
    15781 aggtgcgcct tgtggagaga gcttttggca ttgggccctc ggcgctgacg gaggccatgg
    15841 gcatgctcaa ccaccatttc gcagcagccg cctgatcggc gcagagcgac agcctacctc
    15901 tgactgccgc caatctttgc aacagagcc
//
